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| 1 | Synergistic defensive mechanism of phytochelatins and antioxidative enzymes in Brassica chinensis L. against Cd stress显示文摘Brassica chinensis L. was chosen and exposed to different concentrations of Cd exposure to evaluate its Cd-accumulating capacity and its potential cellular defensive mechanisms. Cd accumulation in the shoots and roots of B. chinensis was up to 1348.3±461.8 and 3761.0±795.0 mg per killogram of dry weight, respectively, under 200 μmol/L of Cd exposure. Increasing Cd accumulation in the plant was accompanied by rapid accumulation of phytochelatins (PCs), and the sequestration of Cd by PCs provided a primary cellular mechanism for Cd detoxification and tolerance of B. chinensis. Furthermore, malondialdehyde formation, hydrogen peroxide content and antioxidative enzyme activities such as superoxide dismutase, catalase, guaiacol peroxidase and ascorbate peroxidase were observed in the shoots of Cd-stressed B. chinensis. Increasing enzyme activities in response to concentrations of 5 to 50 μmol/L Cd showed an efficient defense against oxidative stress, suggesting that the antioxidative system was a secondary defensive mechanism. These resulted in reduced free Cd damage and enhanced Cd accumulation and tolerance. Glutathione plays a pivotal role in these two detoxification pathways. In general, these results suggested that PCs and the antioxidative system are synergistic in combatting Cd-induced oxidative stress and that they play important roles in Cd detoxification of B. chinensis, and also give a deep understanding of the natural defensive mechanisms in plants under heavy metal stress. | CHEN LiQin GUO YiFei YANG LiMin WANG QiuQuan | 2008 | Chinese Science Bulletin2008，53，10： | 8 |
| 2 | Studies of biouptake and transformation of mercury by a typical unicellular diatom Phaeodactylum tricornutum显示文摘Mercury(Hg) is a toxic heavy metal with its biogeochemical cycling in the ocean depending on the type and behavior of the oceanic microalgae.The present work aimed to evaluate bioaccumulation and transformation of Hg by Phaeodactylum tricornutum,a typical unicellular diatom,when exposed to the extremely high level of Hg in order to understand the possible mechanisms of acute stress response.P.tricornutum can accumulate Hg(its bioaccumulation factor is at 104 level),and the 96 h EC 50 was estimated to be 145μg L-1.The amounts of surface-bound Hg being about 1.2 to 4.8 times higher than those of intracellular Hg under exposure to HgCl 2(from 20 to 120μg L-1 concentrations) suggested that the cell wall of P.tricornutum is an important 'fence' towards Hg.After entering the P.tricornutum cell,Hg underwent transformation in its chemical form via interactions with high molecular weight sulfur-containing proteins(accounting for 68% of the intracellular Hg),and glutathione as well as the induced phytochelatins(PCs)(24% Hg) which alleviated the toxicity of HgCl2.In addition,the existence of organic ligands greatly influenced the uptake and transformation behavior of P.tricornutum towards HgCl2,especially in the case of cysteine(Cys),which increased the uptake of Hg,but alleviated the toxicity of Hg towards P.tricornutum due to the fact that Cys is an important precursor for the synthesis of PCs inside the cell.The uptake process of Hg by P.tricornutum was in agreement with the Freundlich isotherm,suggesting a typical heterogeneous sorption process.More importantly,we observed the conversion of HgCl2 into methylmercury inside the P.tricornutum cells and its release into the culture solution using HPLC/CVG-AFS and GC-MS,although the mechanism needs to be further investigated. | DENG GuiFu ZHANG TianWen YANG LiMin WANG QiuQuan | 2013 | Chinese Science Bulletin2013，58，2： | 7 |
| 3 | Low-latency deep-reinforcement learning algorithm for ultrafast fiber lasers显示文摘The application of machine learning to the field of ultrafast photonics is becoming more and more extensive.In this paper,for the automatic mode-locked operation in a saturable absorber-based ultrafast fiber laser(UFL),a deep-reinforcement learning algorithm with low latency is proposed and implemented.The algorithm contains two actor neural networks providing strategies to modify the intracavity lasing polarization state and two critic neural networks evaluating the effect of the actor networks.With this algorithm,a stable fundamental modelocked(FML)state of the UFL is demonstrated.To guarantee its effectiveness and robustness,two experiments are put forward.As for effectiveness,one experiment verifies the performance of the trained network model by applying it to recover the mode-locked state with environmental vibrations,which mimics the condition that the UFL loses the mode-locked state quickly.As for robustness,the other experiment,at first,builds a database with UFL at different temperatures.It then trains the model and tests its performance.The results show that the average mode-locked recovery time of the trained network model is 1.948 s.As far as we know,it is 62.8%of the fastest average mode-locked recovery time in the existing work.At different temperatures,the trained network model can also recover the mode-locked state of the UFL in a short time.Remote algorithm training and automatic mode-locked control are proved in this work,laying the foundation for long-distance maintenance and centralized control of UFLs. | QIUQUAN YAN QINGHUI DENG JUN ZHANG YING ZHU KE YIN TENG LI DAN WU TIAN JIANG | 2021 | Photonics Research2021，9，8： | 7 |
| 4 | Distribution of lanthanum among the chloroplast subcomponents of spinach and its biological effects on photosynthesis:location of the lanthanum binding sites in photosystem II显示文摘The effects of lanthanum at different concen-trations on the related photosynthetic activities of Hill reac-tion, Mg2+-ATPase and Ca2+-ATPase in spinach chloroplast were studied. Experimental results showed that lanthanum can increase all the activities at suitable concentration (15-30 mg·L-1), however, it behaves toxically on them when over used (60 mg·L-1). To get an improved understanding of the mechanism of lanthanum effects on the photosynthesis of spinach, the different subcomponents in the chloroplast of the cultured spinach were isolated, and the content of lan-thanum in each subcomponent was determined by ICP-MS. The results obtained indicated that among these different subcomponents, about 90% out of the total chloroplast lan-thanum was located in photosystem II (PS II) while there was little lanthanum in photosystem I (PS I). Moreover, size ex-clusion high performance liquid chromatography (SE-HPLC) coupled with online UV and ICP-MS detections was novelly used for locating lanthanum binding sites in PS II proteins for the first time. It was found that lanthanum has two bind-ing sites in PS II: La associates with chlorophyll together with magnesium in PS II by partly replacing magnesium and also shares the common binding sites of PS II proteins to-gether with the inorganic cofactors of calcium and manga-nese, influencing the process of photosynthesis. | YAN Wenwu YANG Limin WANG Qiuquan | 2005 | Chinese Science Bulletin2005，50，16： | 5 |
| 5 | Bioaccumulation and transformation of cadmium by Phaeodactylum tricornutum显示文摘In this paper,we report the bioaccumulation and transformation of cadmium (Cd) by Phaeodactylum tricornutum in the presence of ethylenediamine tetra acetic acid (EDTA) and cysteine (Cys). Both EDTA and Cys can alleviate the toxicity of Cd to P. tricornutum. Short term intracellular uptake and extracellular adsorption experiments using ICP-MS indicated that the amounts of Cd accumulated on the cell surface of P. tricornutum and inside the cell decreased along with the increase of EDTA concentration,which conformed to the prediction of the Free Ion Activity Model (FIAM). However,extracellular adsorption of Cd increased at first and then decreased along with the increase in the concentration of Cys,while intracellular uptake increased under Cys concentrations from the blank value to 4.45 μmol/L,and then tended to remain at the same level when the Cys concentration was greater than 4.45 μmol/L,and this deviated remarkably from the FIAM. The interactions of Cd with _Si_OH,_C_OH and NH2(CO) _OH on the cell wall were confirmed using FT-IR and XPS studies. The results obtained using HPLC of the phytochelatins (PCs) produced by P. tricornutum under CdCl2,Cd_EDTA and Cd_Cys stress suggested that the main reason for the different effects of EDTA and Cys on the bioaccumulation and transformation of Cd by P. tricornutum was that Cys is not only a complexing ligand to Cd,as is EDTA,but also it is a precursor of the intracellular synthesizing PCs participating in the cellular defense mechanism against Cd. Furthermore,the discovery of in vivo PCs and oxidized_PCs as well as Cd-PC2 in P. tricornutum using ESI-IT-MS provided the evidence for deactivation of Cd by the PCs,reducing Cd-toxicity to P. tricornutum. | SI DongFang YANG LiMin YAN Hua WANG QiuQuan | 2009 | Science China Chemistry2009，52，12： | 3 |
| 6 | Integrated microdevice with a windmill-like hole array for the clog-free,efficient,and self-mixing enrichment of circulating tumor cells显示文摘Circulating tumor cells(CTCs)have tremendous potential to indicate disease progression and monitor therapeutic response using minimally invasive approaches.Considering the limitations of affinity strategies based on their cost,effectiveness,and simplicity,size-based enrichment methods that involve low-cost,label-free,and relatively simple protocols have been further promoted.Nevertheless,the key challenges of these methods are clogging issues and cell aggregation,which reduce the recovery rates and purity.Inspired by the natural phenomenon that the airflow around a windmill is disturbed,in this study,a windmill-like hole array on the SU-8 membrane was designed to perturb the fluid such that cells in a fluid would be able to self-mix and that the pressure acting on cells or the membrane would be dispersed to allow a greater velocity.In addition,based on the advantages of fluid coatings,a lipid coating was used to modify the membrane surface to prevent cell aggregation and clogging of the holes.Under the optimal conditions,recovery rates of 93%and 90%were found for A549 and HeLa cells in a clinical simulation test of our platform with a CTC concentration of 20-100 cells per milliliter of blood.The white blood cell(WBC)depletion rate was 98.7%(n=15),and the CTC detection limit was less than 10 cells per milliliter of blood(n=6).Moreover,compared with conventional membrane filtration,the advantages of the proposed device for the rapid(2 mL/min)and efficient enrichment of CTCs without clogging were shown both experimentally and theoretically.Due to its advantages in the efficient,rapid,uniform,and clog-free enrichment of CTCs,our platform offers great potential for metastatic detection and therapy analyses. | Hao Li Jinze Li Zhiqi Zhang Zhen Guo Changsong Zhang Zixu Wang Qiuquan Guo Chao Li Chuanyu Li Jia Yao Anran Zheng Jingyi Xu Qingxue Gao Wei Zhang Lianqun Zhou | 2022 | Microsystems & Nanoengineering2022，8，1： | 1 |
| 7 | Speciation of rare earth elements in soil by sequential extraction then HPLC coupled with visible and ICP–MS detection显示文摘 | Qiuquan Wang B. Huang Zhenwei Guan Limin Yang Bin Li | 2001 | Fresenius’ Journal of Analytical Chemistry2001，，8： | 1 |
| 8 | Modeling persis- tent organic pollutant partitioning between tree bark and air and its application to spatial monitoring of atmospheric POPs in mainland China显示文摘 | Zhao Yuli Yang Limin Wang Qiuquan | 2008 | Environ Sci Technol2008，42，： | 1 |
| 9 | Chromatographic behavior of cadmium in an ion-pair reversed-phase micro HPLC system and its application to the determination of bio-available cadmium in soil samples显示文摘 | Bin Li Qiuquan Wang Hua Yan Limin Yang Benli Huang | 2003 | Analytical and Bioanalytical Chemistry2003，，6： | 1 |
| 10 | Evaluation of the results from a quasi-tessier's sequential extraction procedure for heavy metal speciation in soils and sediment by ICP- MS显示文摘 | LI Bin WANG Qiuquan HUANG Benli | 2001 | Anal Sci2001，，： | 1 |
| 11 | Key progresses of MOE key laboratory of macromolecular synthesis and functionalization in 2021显示文摘In 2021,The MOE Key Laboratory of Macromolecular Synthesis and Functionalization in Zhejiang University had achieved several important results.First,a series of versatile organoboron catalysts were synthesized for ring-opening(co)polymerizations.Second,a catalyst-free polycondensation mechanism was proposed for the production of polyesters with high molecular weights.Third,a co-assembly method that can fabricate films and coatings with controllable structures and properties on various substrates was demonstrated,providing a platform for the construction of novel surface coatings.Forth,facile methods for producing high-productivity poly(propylene carbonate)and semicrystalline polyester have been discovered.And linear non-conjugated polyesters exhibiting yellow-green clusteroluminescence were developed for the first time.Fifth,a supramolecular prodrug nano-assembly strategy has been developed for reactive nitrogen species potentiated chemotherapy.Sixth,a series of tough and stiff supramolecular hydrogels with shape memory properties have been used for information encryption.Seventh,reversible fusion and fission of wet-spun graphene oxide fibers has been successfully achieved.Eighth,three non-conjugated polypeptides were synthesized and the mechanism of clusteroluminescence was studied.Ninth,a series of conducting covalent organic frameworks with high electrical conductivity and carrier mobility have been used as high-performance chemiresistor,electrocatalyst,and organic field-effect transistor.Tenth,the exploration of non-fused electron acceptors,and their photostable mechanism are exemplified for developing high-performance,low-cost and eco-friendly polymer solar cells.Finally,gel-grown long-range ordering bulk-heterojunctions has achieved improved X-ray detector performance. | Quan Wen Qiuquan Cai Ping Fu Dan Chang Xiaoyi Xu Tian-Jiao Wen Guang-Peng Wu Weipu Zhu Ling-Shu Wan Chengjian Zhang Xing-Hong Zhang Qiao Jin Zi-Liang Wu Chao Gao Haoke Zhang Ning Huang Chang-Zhi Li Hanying Li | 2023 | Chinese Chemical Letters2023，34，5： | 1 |
| 12 | Speciation of rare earth elements in soil by sequential extraction then HPLC coupled with visible and ICP-MS detection 显示文摘 | Wang Qiuquan Huang Benli Guan Zhenwei Yang Limin Li Bin | 2001 | Fresenius'' J Anal Chem2001，，370： | 1 |
| 13 | dentification of AtOPT4 as a Plant Glutathione ;ransporter显示文摘Dear Editor, Glutathione (GSH) is an essential tri-peptide important for several plant processes,including the control of cellular redox status,detoxification of xenobiotics,root development,heavy metal transport and resistance,and long-distance transport of organic sulfur.Cadmium and arsenic are detoxified by small GSH-derived peptides,called phytochelatins (PCs). | Zhongchun Zhang Qingqing Xie Timothy O. Jobe Andrew R. Kau Cun Wang Yunxia Li Baosheng Qiu Qiuquan Wang David G. Mendoza-Cdzatl Julian I. Schroeder | 2016 | Molecular Plant2016，9，3： | 0 |
| 14 | Tunable Fluid-Type Metasurface for Wide-Angle and Multifrequency Water-Air Acoustic Transmission显示文摘Efficient acoustic communication across the water-air interface remains a great challenge owing to the extreme acoustic impedance mismatch.Few present acoustic metamaterials can be constructed on the free air-water interface for enhancing the acoustic transmission because of the interface instability.Previous strategies overcoming this difficulty were limited in practical usage,as well as the wide-angle and multifrequency acoustic transmission.Here,we report a simple and practical way to obtain the wide-angle and multifrequency water-air acoustic transmission with a tunable fluid-type acoustic metasurface(FAM).The FAM has a transmission enhancement of acoustic energy over 200 times,with a thickness less than the wavelength in water by three orders of magnitude.The FAM can work at an almost arbitrary water-to-air incident angle,and the operating frequencies can be flexibly adjusted.Multifrequency transmissions can be obtained with multilayer FAMs.In experiments,the FAM is demonstrated to be stable enough for practical applications and has the transmission enhancement of over 20 dB for wide frequencies.The transmission enhancement of music signal across the water-air interface was performed to demonstrate the applications in acoustic communications.The FAM will benefit various applications in hydroacoustics and oceanography. | Zhandong Huang Shengdong Zhao Yiyuan Zhang Zheren Cai Zheng Li Junfeng Xiao Meng Su Qiuquan Guo Chuanzeng Zhang Yaozong Pan Xiaobing Cai Yanlin Song Jun Yang | 2022 | Research2022，，1： | 0 |
| 15 | Tunable Fluid-Type Metasurface for Wide-Angle and Multifrequency Water-Air Acoustic Transmission显示文摘Efficient acoustic communication across the water-air interface remains a great challenge owing to the extreme acoustic impedance mismatch.Few present acoustic metamaterials can be constructed on the free air-water interface for enhancing the acoustic transmission because of the interface instability.Previous strategies overcoming this difficulty were limited in practical usage,as well as the wide-angle and multifrequency acoustic transmission.Here,we report a simple and practical way to obtain the wide-angle and multifrequency water-air acoustic transmission with a tunable fluid-type acoustic metasurface(FAM).The FAM has a transmission enhancement of acoustic energy over 200 times,with a thickness less than the wavelength in water by three orders of magnitude.The FAM can work at an almost arbitrary water-to-air incident angle,and the operating frequencies can be flexibly adjusted.Multifrequency transmissions can be obtained with multilayer FAMs.In experiments,the FAM is demonstrated to be stable enough for practical applications and has the transmission enhancement of over 20 dB for wide frequencies.The transmission enhancement of music signal across the water-air interface was performed to demonstrate the applications in acoustic communications.The FAM will benefit various applications in hydroacoustics and oceanography. | Zhandong Huang Shengdong Zhao Yiyuan Zhang Zheren Cai Zheng Li Junfeng Xiao Meng Su Qiuquan Guo Chuanzeng Zhang Yaozong Pan Xiaobing Cai Yanlin Song Jun Yang | 2021 | Research2021，，1： | 0 |
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