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| 1 | Upregulation of Chemokine CXCL12 in the Dorsal Root Ganglia and Spinal Cord Contributes to the Development and Maintenance of Neuropathic Pain Following Spared Nerve Injury in Rats显示文摘Emerging evidence indicates that CXCL12/CXCR4 signaling is involved in chronic pain. However, few studies have systemically assessed its role in direct nerve injury-induced neuropathic pain and the underlying mechanism. Here, we determined that spared nerve injury(SNI)increased the expression of CXCL12 and its cognate receptor CXCR4 in lumbar 5 dorsal root ganglia(DRG)neurons and satellite glial cells. SNI also induced longlasting upregulation of CXCL12 and CXCR4 in the ipsilateral L4–5 spinal cord dorsal horn, characterized by CXCL12 expression in neurons and microglia, and CXCR4 expression in neurons and astrocytes. Moreover, SNIinduced a sustained increase in TNF-a expression in the DRG and spinal cord. Intraperitoneal injection(i.p.) of the TNF-a synthesis inhibitor thalidomide reduced the SNI-induced mechanical hypersensitivity and inhibited the expression of CXCL12 in the DRG and spinal cord.Intrathecal injection(i.t.) of the CXCR4 antagonist AMD3100, both 30 min before and 7 days after SNI,reduced the behavioral signs of allodynia. Rats given an i.t.or i.p. bolus of AMD3100 on day 8 of SNI exhibited attenuated abnormal pain behaviors. The neuropathic pain established following SNI was also impaired by i.t. administration of a CXCL12-neutralizing antibody. Moreover,repetitive i.t. AMD3100 administration prevented the activation of ERK in the spinal cord. The mechanical hypersensitivity induced in na?¨ve rats by i.t. CXCL12 was alleviated by pretreatment with the MEK inhibitor PD98059. Collectively, our results revealed that TNF-a might mediate the upregulation of CXCL12 in the DRG and spinal cord following SNI, and that CXCL12/CXCR4 signaling via ERK activation contributes to the development and maintenance of neuropathic pain. | Liying Bai Xinru Wang Zhisong Li Cunlong Kong Yonghui Zhao Jun-Liang Qian Quancheng Kan Wei Zhang Ji-Tian Xu | 2016 | Neuroscience Bulletin2016，32，1： | 20 |
| 2 | Circulating tumor DNA 5-hydroxymethylcytosine as a novel diagnostic biomarker for esophageal cancer显示文摘 | Xin Tian Baofa Sun Chuanyuan Chen Chunchun Gao Ji Zhang XingYu Lu Linchen Wang Xiangnan Li Yurong Xing Ruijuan Liu Xiao Han Zheng Qi Xiaojian Zhang Chuan He Dali Han Yun-Gui Yang Quancheng Kan | 2018 | Cell Research2018，28，5： | 12 |
| 3 | Intrinsic apoptotic pathway and G2/M cell cycle arrest involved in tubeimoside I-induced EC109 cell death显示文摘Objective: Squamous esophageal carcinoma is highly prevalent in developing countries, especially in China. Tu Bei Mu (TBM), a traditional folk medicine, has been used to treat esophageal squamous cell carcinoma (ESCC) for a long term. tubeimoside I (TBMS1) is the main component of TBM, exhibiting great anticancer potential. In this study, we investigated the mechanism of TBMS1 cytotoxic effect on EC109 cells. Methods: Comparative nuclear proteomic approach was applied in the current study and we identified several altered protein spots. Further biochemical studies were carried out to detect the mitochondrial membrane potential, cell cycle and corresponding proteins' expression and location. Results: Subcellular proteomic study in the nucleus from EC109 cells revealed that altered proteins were associated with mitochondrial function and cell proliferation. Further biochemical studies showed that TBMS1-induced molecular events were related to mitochondria-induced intrinsic apoptosis and P21-cyclin B1/cdc2 complex-related G2/M cell cycle arrest. Conclusions: Considering the conventional application of TBM in esophageal cancer, TBMS1 therefore may have a great potential as a chemotherapeutic drug candidate for ESCC. | Yang Xu Guanghui Wang Quancheng Chen Ting Lin Zhiping Zeng Qiang Luo Jie Liu Cuiling Sun | 2013 | Chinese Journal of Cancer Research2013，25，3： | 12 |
| 4 | The resurgent landscape of xenotransplantation of pig organs in nonhuman primates显示文摘Organ shortage is a major bottleneck in allotransplantation and causes many wait-listed patients to die or become too sick for transplantation.Genetically engineered pigs have been discussed as a potential alternative to allogeneic donor organs.Although xenotransplantation of pig-derived organs in nonhuman primates(NHPs)has shown sequential advances in recent years,there are still underlying problems that need to be completely addressed before clinical applications,including(i)acute humoral xenograft rejection;(ii)acute cellular rejection;(iii)dysregulation of coagulation and inflammation;(iv)physiological incompatibility;and(v)cross-species infection.Moreover,various genetic modifications to the pig donor need to be fully characterized,with the aim of identifying the ideal transgene combination for upcoming clinical trials.In addition,suitable pretransplant screening methods need to be confirmed for optimal donor-recipient matching,ensuring a good outcome from xenotransplantation.Herein,we summarize the understanding of organ xenotransplantation in pigs-to-NHPs and highlight the current status and recent progress in extending the survival time of pig xenografts and recipients.We also discuss practical strategies for overcoming the obstacles to xenotransplantation mentioned above to further advance transplantation of pig organs in the clinic. | Xuan Zhang Quancheng Wang Jingjun Zhao Xiao Li Wei Peng Zhaoxu Yang Zhibin Lin Long Yang Rui Ding Kaishan Tao Kefeng Dou | 2021 | Science China(Life Sciences)2021，64，5： | 10 |
| 5 | Optimization of microwave-assisted extraction of polysaccharides in the flower of Platycodon grandiflorum by response surface methodology显示文摘Platycodon grandiflorum polysaccharides(PGPs)are naturally occurring compounds derived from the flowers of platycodon grandiflorum.the microwave assisted extraction(MAE)of PGPs was studied.A three-level and three-factor Box-Behnken design was used to optimize MAE conditions,and the effects of operating conditions including microwave power(180-540 W),extraction time(20-60 s)and liquid to solid ratio(20-30 v/w)on the extraction yield of PGPs were studied through response surface methodology(RSM).The results showed that the highest yield of PGPs reached 8.35 g/(100 g)at 360 W microwave power,40 s extraction time and 25.2 v/w liquid to solid ratio.The extraction yield of PGPs was significantly affected by liquid to solid ratio(P<0.01),microwave power and extraction time(P<0.05).Compared with the conventional solvent extraction,MAE is more efficient and rapid due to the disruption of the flower tissue microstructure as a result of microwave acoustic cavitation as observed with the scanning electron microscopy(SEM).A macroporous resin,HPD400,was found to be effective for purifying the PGPs extract. | Li Lei Zhang Jinping Zhou Quancheng | 2009 | International Journal of Agricultural and Biological Engineering2009，2，2： | 5 |
| 6 | Involvement of long non-coding RNAs in the progression of esophageal cancer显示文摘Esophageal cancer(EC)is one of the most common malignant tumors of the digestive system with high incidence and mortality rate worldwide.Therefore,exploring the pathogenesis of ECand searching for new targeted therapies are the current research hotspot for EC treatment.Long non-coding RNAs(lncRNAs)are endogenous RNAs with more than 200 nucleotides,but without proteincoding function.In recent years,lncRNAs have gradually become the focuses in the field of non-coding RNA.Some lncRNAs have been proved to be closely related to the pathogenesis of EC.Many lncRNAs are abnormally expressed in EC and participate in many biological processes including cell proliferation,apoptosis,and metastasis by inhibiting or promoting target gene expression.LncRNAs can also regulate the progression of EC through epithelial-mesenchymal transformation(EMT),which is closely related to the occurrence,development,and prognosis of EC.In this article,we review and discuss the involvement of lncRNAs in the progression of EC. | Wenhua Xue Yuanyuan Zheng Zhibo Shen Lifeng Li Zhirui Fan Wenbin Wang Zijia Zhu Yunkai Zhai Jie Zhao Quancheng Kan | 2021 | Cancer Communications2021，41，5： | 4 |
| 7 | Effect of oxymatrine on interferon-gamma and tumor necrosis factor-alpha serum levels in an experimental rat model of autoimmune encephalomyelitis显示文摘BACKGROUND:Studies have demonstrated that experimental autoimmune encephalomyelitis(EAE) onset correlates with increased interferon-γ(IFN-γ) and tumor necrosis factor-α(TNF-α) expression.Oxymatrine(OM) has been shown to inhibit autoimmune responses,but there are no reports showing that it could prevent the development of EAE.OBJECTIVE:To observe the effect of OM on serum levels of IFN-γ and TNF-α in a rat model of EAE.DESIGN,TIME AND SETTING:A randomized,controlled,animal study was performed at the Experimental Animal Center of Henan Academy of Chinese Medicine and at the Key Disciplines Laboratory Clinical Medicine of Henan Province between July and December 2008.MATERIALS:OM was purchased from Chia-tai Tianqing Pharmaceutical,China;complete Freund's adjuvant was purchased from Sigma,USA.METHODS:Forty female Wistar rats were randomly assigned to four groups:EAE model(M),low-dose OM treatment(OM-L),high-dose OM treatment(OM-H),and normal control(N,no immunization),with 10 rats in each group.EAE was established in the M,OM-L,and OM-H groups following immunization with Guinea pig spinal cord homogenate and complete Freund's adjuvant.The M and N groups were intraperitoneally injected with normal saline(6.7 mL/kg per day),the OM-L group received an intraperitoneal injection of OM(100 mg/kg per day),and the OM-H group received OM(150 mg/kg per day).MAIN OUTCOME MEASURES:At 16 days after immunization,the degree of histopathological changes in the spinal cord was assessed by hematoxylin-eosin stanining.Enzyme-linked immunosorbent assay was used to detect serum levels of IFN-γ,and radioimmunoassay was utilized to determine serum TNF-α level.Neurological scores were measured on a daily basis according to a 0-5 scale.RESULTS:Daily injections of OM,both high and low doses,resulted in decreased neurological scores in EAE rats(P < 0.01),as well as reduced cellular infiltration in the spinal cord and decreased levels of serum IFN-γ and TNF-α(P < 0.01).CONCLUSION:OM reduced the onset and severity of EAE,which correlated with decreased IFN-γ and TNF-α expression. | Xiaobin Guo Quancheng Kan Yifan Song Lin Zhu Xiang Li Haiying Hua GuangxianZhang | 2010 | Neural Regeneration Research2010，5，10： | 2 |
| 8 | Analysis of bending on the front end of sheet during hot rolling显示文摘The analysis of bending in finishing rolling was performed. An asymmetrical rolling force model was established, and the upper and lower neutral points were determined. The bending which resulted from the asymmetrical rolling force at the roll gap was studied and related flexibility equations were proposed according to elastic mechanics. Moreover, material mechanics was used to analyze the effects of temperature difference and self-weight on the bending out of the roll gap, and the flexibility equations were constructed. The main factors on bending were summed up, and the bending rule in the rolling could be obtained. In addition practical calculation was made with the production data of ribbon steel from Laiwu Iron & Steel Group Co. Ltd. | Jiquan Sun Haibin Zhang Quancheng Yu | 2006 | Journal of University of Science and Technology Beijing2006，13，1： | 2 |
| 9 | Optimization of supercritical fluid extraction of daidzein from soybean after extrusion显示文摘A set of optimal conditions for supercritical fluid extraction(SFE)of daidzein in soybean previously subjected to extrusion at the rotate speed of screw 4.5×g and the temperature of 80℃was developed.The quadratic orthogonal rotation design with five factors(extracting temperature,extracting time,ethanlol content,cosolvent quantity and flow rate)was used and a mathematical model was developed based the experimental results.Optimal conditions were verified and compared with control experiments conducted in parallel.The results from SFE showed that all five experiment factors had significant effects on the yield of daidzein.The mathematical model agreed well with the experimental results.It was found that there were 27 optimal extract conditions under which daidzein yield was higher than 0.092%.The optimal extracting conditions were stable and reproducible.Results from the control experiments showed that extrusion could significantly enhance the extraction of daidzein.The extraction of daidzein from extruded soybean and its products with supercritical CO_(2) is a potential technique for practical applications in the future. | Zhou Quancheng Li Lei Shen Dechao | 2009 | International Journal of Agricultural and Biological Engineering2009，2，2： | 2 |
| 10 | Heparin inhibits burn-induced spleen cell apoptosis by suppressing interleukin-1 expression显示文摘 | Zhao Songfeng Zhang Xiao Zhang Xiaojian Shi Xiuqin Yu Zujiang Kan Quancheng | 2014 | Chinese Medical Journal2014，，13： | 1 |
| 11 | Concentration of tocopherols by supercritical carbon dioxide with cosolvents 显示文摘 | Zhou Quancheng Sheng Guihua Jiang Hong | 2004 | European Food Research and Technology2004，219，4： | 1 |
| 12 | Semi-con- tinuous anaerobic co-digestion of thickened waste activated sludge and fat, oil and grease显示文摘 | Wan Caixia Zhou Quancheng Fu Guiming | 2011 | Waste Management2011，31，： | 1 |
| 13 | Ammonia-induced energy disorders interfere with bilirubin metabolism in hepatocytes显示文摘 | Qiongye Wang Yanfang Wang Zujiang Yu Duolu Li Bin Jia Jiangjing Li Kelei Guan Yubing Zhou Yanling Chen Quancheng Kan | 2014 | Archives of Biochemistry and Biophysics2014，，： | 1 |
| 14 | NSUN2-mediated mRNA m^(5)C Modification Regulates the Progression of Hepatocellular Carcinoma显示文摘RNA modifications affect many biological processes and physiological diseases.The 5-methylcytosine(m^(5)C)modification regulates the progression of multiple tumors.However,its characteristics and functions in hepatocellular carcinoma(HCC)remain largely unknown.Here,we found that HCC tissues had a higher m^(5)C methylation level than the adjacent normal tissues.Transcriptome analysis revealed that the hypermethylated genes mainly participated in the phosphokinase signaling pathways,such as the Ras and PI3K-Akt pathways.The m^(5)C methyltransferase NSUN2 was highly expressed in HCC tissues.Interestingly,the expression of many genes was positively correlated with the expression of NSUN2,including GRB2,RNF115,AATF,ADAM15,RTN3,and HDGF.Real-time PCR assays further revealed that the expression of the mRNAs of GRB2,RNF115,and AATF decreased significantly with the down-regulation of NSUN2 expression in HCC cells.Furthermore,NSUN2 could regulate the cellular sensitivity of HCC cells to sorafenib via modulating the Ras signaling pathway.Moreover,knocking down NSUN2 caused cell cycle arrest.Taken together,our study demonstrates the vital role of NSUN2 in the progression of HCC. | Dan Song Ke An Wenlong Zhai Luyao Feng Yingjie Xu Ran Sun Yueqin Wang Yun-Gui Yang Quancheng Kan Xin Tian | 2023 | Genomics, Proteomics & Bioinformatics2023，21，4： | 1 |
| 15 | The icp etching technology of 3C-SiC films显示文摘 | Jin N Quancheng G Guosheng S | 2006 | J Phys Conference Series2006，34，1： | 1 |
| 16 | RNA Structural Dynamics Modulate EGFR-TKI Resistance Through Controlling YRDC Translation in NSCLC Cells显示文摘Epidermal growth factor receptor-tyrosine kinase inhibitors(EGFR-TKIs)positively affect the initial control of non-small cell lung cancer(NSCLC).Rapidly acquired resistance to EGFR-TKIs is a major hurdle in successful treatment.However,the mechanisms that control the resistance of EGFR-TKIs remain largely unknown.RNA structures have widespread and crucial functions in many biological regulations;however,the functions of RNA structures in regulating cancer drug resistance remain unclear.Here,the psoralen analysis of RNA interactions and structures(PARIS)method is used to establish the higher-order RNA structure maps of EGFRTKIs-resistant and-sensitive cells of NSCLC.Our results show that RNA structural regions are enriched in untranslated regions(UTRs)and correlate with translation efficiency(TE).Moreover,yrdC N6-threonylcarbamoyltransferase domain containing(YRDC)promotes resistance to EGFR-TKIs.RNA structure formation in YRDC 30 UTR suppresses embryonic lethal abnormal vision-like 1(ELAVL1)binding,leading to EGFR-TKI sensitivity by impairing YRDC translation.A potential therapeutic strategy for cancer treatment is provided using antisense oligonucleotide(ASO)to perturb the interaction between RNA and protein.Our study reveals an unprecedented mechanism through which the RNA structure switch modulates EGFR-TKI resistance by controlling YRDC mRNA translation in an ELAVL1-dependent manner. | Boyang Shi Ke An Yueqin Wang Yuhan Fei Caixia Guo Qiangfeng Cliff Zhang Yun-Gui Yang Xin Tian Quancheng Kan | 2023 | Genomics, Proteomics & Bioinformatics2023，21，4： | 1 |
| 17 | Semi-continuous anaerobic co-digestion of thickened waste activated sludge and fat, oil and grease显示文摘 | Caixia Wan Quancheng Zhou Guiming Fu Yebo Li | 2011 | Waste Management2011，，8： | 1 |
| 18 | CYP3A4 * 1G genetic polymorphism influences CYP3A activity and response to fentanyl in Chinese gynecologic patients显示文摘 | ZHANG Wei CHANG Yanzi KAN Quancheng | 2010 | Eur J Clin Pharmacol2010，66，1： | 1 |
| 19 | MiR-9 promotes the neural differentiation of mouse bone marrow mesenchymal stem cells via targeting zinc finger protein 521 显示文摘 | HAN Rui KAN Quancheng SUN Yingpu | 2012 | Neurosci Lett2012，515，2： | 1 |
| 20 | A Study on the Pyrolytic Characteristics of Xanthoceras Sorbifolia Husk and Its Crude Cellulose Extract显示文摘Huge amounts of Xanthoceras sorbifolia husks(XSH)are typically discarded after oil extraction.Since pyrolysis represents a promising solution to harness the bio-energy of XSH,in the present work the pyrolytic and kinetic characteristics of XSH and related crude cellulose extract(CCE)were studied considering different rates of heating(10,30 and 50℃ min^(-1)).The pyrolysis activation energy,pre-exponential factors and mechanism function were computed using different models namely Popescu,FWO(Flynn-Wall-Ozawa)and KAS(Kissinger-Akahira-Sunose).The pyrolysis process was articulated into three stages:dehydration(Stage I),primary devolatilization(Stage II),residual decomposition(Stage III).Marked variations in the average activation energy,thermal stability,final residuals and rate of reaction were noted.Stage II of XSH and CCE could be described by the Avramic-Erofeev equations.The average activation energies of XSH and CCE were found to be 269 and 296 kJ mol^(-1),respectively. | Hongmei Zhang Quancheng Zhou | 2021 | Fluid Dynamics & Materials Processing2021，17，6： | 0 |
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