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| 1 | Hypoxia-stressed cardiomyocytes promote early cardiac differentiation of cardiac stem cells through HIF-1α/Jagged1/Notch1 signaling显示文摘Hypoxia is beneficial for the differentiation of stem cells transplanted for myocardial injury,but mechanisms underlying this benefit remain unsolved. Here, we report the impact of hypoxia-induced Jagged1 expression in cardiomyocytes(CMs) for driving the differentiation of cardiac stem cells(CSCs).Forced hypoxia-inducible factor 1α(HIF-1α) expression and physical hypoxia(5% O_2) treatment could induce Jagged1 expression in neonatal rat CMs. Pharmacological inhibition of HIF-1α by YC-1 attenuated hypoxia-promoted Jagged1 expression in CMs. An ERK inhibitor(PD98059), but not inhibitors of JNK(SP600125), Notch(DAPT), NF-κB(PTDC), JAK(AG490), or STAT3(Stattic) suppressed hypoxiainduced Jagged1 protein expression in CMs. c-Kit^+ CSCs isolated from neonatal rat hearts using a magnetic-activated cell sorting method expressed GATA4, SM22α or vWF, but not Nkx2.5 and cTnI.Moreover, 87.3% of freshly isolated CSCs displayed Notch1 receptor expression. Direct co-culture of CMs with BrdU-labeled CSCs enhanced CSCs differentiation, as evidenced by an increased number of BrdU^+/Nkx2.5^+ cells, while intermittent hypoxia for 21 days promoted co-culture-triggered differentiation of CSCs into CM-like cells. Notably, YC-1 and DAPT attenuated hypoxia-induced differentiation.Our results suggest that hypoxia induces Jagged1 expression in CMs primarily through ERK signaling,and facilitates early cardiac lineage differentiation of CSCs in CM/CSC co-cultures via HIF-1α/Jagged1/Notch signaling. | Keke Wang Ranran Ding Yanping Ha Yanan Jia Xiaomin Liao Sisi Wang Rujia Li Zhihua Shen Hui Xiong Junli Guo Wei Jie | 2018 | Acta Pharmaceutica Sinica B2018，8，5： | 7 |
| 2 | Influence of order degree of coaly graphite on its structure change during preparation of graphene oxide显示文摘Coaly graphite is an important natural graphite resource that derived from coal under a natural process that associated with igneous intrusion.Flake graphite is usually used for the chemical synthesis of graphene oxide(GO),the main precursor for preparation of graphene,but few papers pay attention to preparing GO using coaly graphite.In this paper,four kinds of natural coaly graphite with different graphitization degrees were exposed to a modified Hummer’s oxidation method to prepare GO.The flake graphite sample was also used for comparison.The results showed that the structural change process from graphite to GO were significantly affected by the graphitization degree of the original coaly graphite.The relationship between yields of GO and graphitization degrees of the coaly graphite was explored.The mechanism of why the graphite with low graphitization degrees cannot be totally oxidized was proposed.Coaly graphite with a graphitization degree of higher than 80% was easier to be oxidized and yielded the same amount of GO as the flake graphite did,suggesting it is the potential substitute for the flake graphite to produce GO in bulk quantities. | Dandan Hou Kuo Li Rujia Ma Qinfu Liu | 2020 | Journal of Materiomics2020，6，3： | 2 |
| 3 | Super enhancers targeting ZBTB16 in osteogenesis protect against osteoporosis显示文摘As the major cell precursors in osteogenesis, mesenchymal stem cells(MSCs) are indispensable for bone homeostasis and development. However, the primary mechanisms regulating osteogenic differentiation are controversial. Composed of multiple constituent enhancers, super enhancers(SEs) are powerful cis-regulatory elements that identify genes that ensure sequential differentiation. The present study demonstrated that SEs were indispensable for MSC osteogenesis and involved in osteoporosis development. Through integrated analysis, we identified the most common SE-targeted and osteoporosis-related osteogenic gene,ZBTB16. ZBTB16, positively regulated by SEs, promoted MSC osteogenesis but was expressed at lower levels in osteoporosis.Mechanistically, SEs recruited bromodomain containing 4(BRD4) at the site of ZBTB16, which then bound to RNA polymerase IIassociated protein 2(RPAP2) that transported RNA polymerase Ⅱ(POL Ⅱ) into the nucleus. The subsequent synergistic regulation of POL Ⅱ carboxyterminal domain(CTD) phosphorylation by BRD4 and RPAP2 initiated ZBTB16 transcriptional elongation, which facilitated MSC osteogenesis via the key osteogenic transcription factor SP7. Bone-targeting ZBTB16 overexpression had a therapeutic effect on the decreased bone density and remodeling capacity of Brd4^(fl/fl)Prx1-cre mice and osteoporosis(OP) models.Therefore, our study shows that SEs orchestrate the osteogenesis of MSCs by targeting ZBTB16 expression, which provides an attractive focus and therapeutic target for osteoporosis. | Wenhui Yu Zhongyu Xie Jinteng Li Jiajie Lin Zepeng Su Yunshu Che Feng Ye Zhaoqiang Zhang Peitao Xu Yipeng Zeng Xiaojun Xu Zhikun Li Pei Feng Rujia Mi Yanfeng Wu Huiyong Shen | 2023 | Bone Research2023，11，3： | 1 |
| 4 | Zinc supplementation partially prevents renal pathological changes in diabetic rats显示文摘 | Yunan Tang Qin Yang Jiayin Lu Xiaolin Zhang Di Suen Yi Tan Litai Jin Jian Xiao Rujia Xie Madhavi Rane Xiaokun Li Lu Cai | 2010 | The Journal of Nutritional Biochemistry2010，，3： | 1 |
| 5 | Accelerated deterioration mechanism of 316L stainless steel in NaCl solution under the intermittent tribocorrosion process显示文摘In this research,the tribocorrosion behavior of 316L stainless steel in simulated seawater was investigated under continuous and intermittent sliding at open circuit potential.The tribocorrosion mechanism was discussed in terms of wear morphologies,mechanical property as well as chemical composition.Meanwhile,microstructure evolution inside the wear track and open circuit potential recorded after sliding were analyzed to quantify the repassivation kinetics and evaluate the impact of the regenerated passive film on wear.The results showed that the wear rate increased under intermittent sliding when the pause time is long enough to repassivate after sliding.Repeated sliding promoted the refinement of the grain inside the sliding area,which was beneficial to the generation of the thicker and more compact passive film inside the wear track.The ruptured passive film often acted as abrasives during subsequent sliding.Therefore,the accelerated material loss under intermittent sliding was attributed to the periodic mechanical removal of the thickened passive film and the enhanced abrasive wear inside the wear track. | Yingrui Liu Linlin Liu Shuyu Li Rujia Wang Peng Guo Aiying Wang Peiling Ke | 2022 | Journal of Materials Science & Technology2022，，26： | 1 |
| 6 | Effects of ambient humidity and thermal aging on properties of Nomex insulation in mining dry‐type transformer显示文摘Temperature and humidity are the main factors that cause the decomposition of Nomex paper widely used as turn‐to‐turn insulation material of mining dry‐type transformers. In this study, for understanding the effect of ambient humidity and thermal aging on the properties of Nomex paper, specimens with the initial moisture content of 7% were aged in the oven for 168, 504, 672 h at 180°C, respectively. After thermal aging, corona discharge experiments of unaged and aged specimens with different aging degrees were carried out at different relative humidity (RH) levels from 10 to 70%. The characteristic parameters of corona discharge, such as the average discharge amplitude and the number of discharges, were measured. Then, the surface conductivity and the surface potential decay rate were measured. The morphology and attenuated total internal reflectance Fourier transform infrared spectroscopy were also analysed for explaining the difference of properties be-tween unaged and aged specimens. The experiment results indicate that the average discharge amplitude decreases with the increase of RH or the deepening of the aging degree of specimens, but the number of discharges shows the reversal tendency. The changes in the physical structure and chemical composition, which is induced by the hydrolysis reaction of Nomex paper, accelerate the surface potential decay and increase the surface conductivity. | Lu Li Jiancheng Song Zhipeng Lei Ailiang Kang Zhengyu Wang Rujia Men Yun Ma | 2021 | High Voltage2021，6，1： | 0 |
| 7 | Mechanisms of glutamate metabolic function and dysfunction in vasculardementia显示文摘As the global population ages,research on the pathogenesis and treatment options for older patients with dementia has become increasingly important.Vascular dementia(VaD),the second most frequent type of dementia,is characterized by vascular impairment caused by inadequate blood supply to the brain.VaD is a complex neurological disorder involving multiple cells and signaling pathways,and its prevention and treatment pose clinical challenges with significant behavioral implications.Glutamate,the most abundant amino acid in the brain,plays a critical role as an excitatory neurotransmitter,impacting cognitive function,learning,and memory.Abnormal glutamate metabolism has been closely linked to dementia,and reduced blood flow to the brain can lead to excessive glutamate accumulation,resulting in neuronal death.This article highlights the connection between VaD and glutamate metabolism,aiming to identify better methods for preventing and treating VaD via regulating glutamate metabolism. | Jiawen Wang Yingmei Zhang Ning Tian Dongshan Ya Jiaxin Yang Yanlin Jiang Xiaoxia Li Xiaohui Lin Bin Yang Qinghua Li Rujia Liao | 2024 | Neuroprotection2024，2，1： | 0 |
| 8 | Effect of long-term fluorination on surface electrical performance of ethylene propylene rubber显示文摘To investigate the effect of fluorination on surface electrical performance of ethylene propylene rubber(EPR),four pieces of EPR specimens are prepared and fluorinated for different duration ranging from 120 min to 480 min.The surface morphology and element compositions of experimental specimens are tested.The surface potential decay and complex permittivity are measured.The tracking discharge property and the erosion properties after tracking test are investigated.The surface charge transport and electrical property tailoring mechanism are discussed.The results show that when the fluorination duration is from 120 min to 360 min,the surface morphology gradually becomes flat and compact with time,and the surface resistance to electrical tracking is improved.While an excessive fluorination with the duration of 480 min leads to a significant increase in surface conductivity and a weakening in the resistance to electrical tracking,which is mainly due to a change in surface morphology.The recommended fluorination duration for an optimised surface property is between 240 and 360 min for EPR specimen. | Rujia Men Zhipeng Lei Tao Han Davide Fabiani Chuanyang Li Simone Vincenzo Suraci Jian Wang | 2019 | High Voltage2019，4，4： | 0 |
| 9 | LncRNA NKILA inhibits HBV replication by repressing NF-κB signalling activation显示文摘Hepatitis B virus(HBV)infection results in liver cirrhosis and hepatocellular carcinoma(HCC).HBx/nuclear factor(NF)-κB pathway plays a role in HBV replication.However,whether NF-κB-interacting long noncoding RNA(NKILA),a suppressor of NF-κB activation,regulates HBV replication remains largely unknown.In this study,gain-and-loss experiments showed that NKILA inhibited HBV replication by inhibiting NF-κB activity.In turn,HBV infection down-regulated NKILA expression.In addition,expression levels of NKILA were lower in the peripheral blood-derived monocytes(PBMCs)of HBV-positive patients than in healthy individuals,which were correlated with HBV viral loads.And a negative correlation between NKILA expression level and HBV viral loads was observed in blood serum from HBV-positive patients.Lower levels of endogenous NKILA were also observed in HepG2 cells expressing a 1.3-fold HBV genome,HBV-infected HepG2-NTCP cells,stable HBV-producing HepG2.2.15 and HepAD38 cells,compared to those HBV-negative cells.Furthermore,HBx was required for NKILA-mediated inhibition on HBV replication.NKILA decreased HBx-induced NF-κB activation by interrupting the interaction between HBx and p65,whereas NKILA mutants lack of essential domains for NF-κB inhibition,lost the ability to inhibit HBV replication.Together,our data demonstrate that NKILA may serve as a suppressor of HBV replication via NF-κB signalling. | Xi Zhang Yuanyuan Li Chen Huan Yubao Hou Rujia Liu Hongyun Shi Peng Zhang Baisong Zheng Yingchao Wang Hong Wang Wenyan Zhang | 2024 | Virologica Sinica2024，39，1： | 0 |
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