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| 1 | CD147-spike protein is a novel route for SARS-CoV-2 infection to host cells显示文摘In face of the everlasting battle toward COVID-19 and the rapid evolution of SARS-CoV-2,no specific and effective drugs for treating this disease have been reported until today.Angiotensin-converting enzyme 2(ACE2),a receptor of SARS-CoV-2,mediates the virus infection by binding to spike protein.Although ACE2 is expressed in the lung,kidney,and intestine,its expressing levels are rather low,especially in the lung.Considering the great infectivity of COVID-19,we speculate that SARS-CoV-2 may depend on other routes to facilitate its infection.Here,we first discover an interaction between host cell receptor CD147 and SARS-CoV-2 spike protein.The loss of CD147 or blocking CD147 in Vero E6 and BEAS-2B cell lines by anti-CD147 antibody,Meplazumab,inhibits SARSCoV-2 amplification.Expression of human CD147 allows virus entry into non-susceptible BHK-21 cells,which can be neutralized by CD147 extracellular fragment.Viral loads are detectable in the lungs of human CD147(hCD147)mice infected with SARS-CoV-2,but not in those of virus-infected wild type mice.Interestingly,virions are observed in lymphocytes of lung tissue from a COVID-19 patient.Human T cells with a property of ACE2 natural deficiency can be infected with SARS-CoV-2 pseudovirus in a dosedependent manner,which is specifically inhibited by Meplazumab.Furthermore,CD147 mediates virus entering host cells by endocytosis.Together,our study reveals a novel virus entry route,CD147-spike protein,which provides an important target for developing specific and effective drug against COVID-19. | Ke Wang Wei Chen Zheng Zhang Yongqiang Deng Jian-Qi Lian Peng Du Ding Wei Yang Zhang Xiu-Xuan Sun Li Gong Xu Yang Lei He Lei Zhang Zhiwei Yang Jie-Jie Geng Ruo Chen Hai Zhang Bin Wang Yu-Meng Zhu Gang Nan Jian-Li Jiang Ling Li Jiao Wu Peng Lin Wan Huang Liangzhi Xie Zhao-Hui Zheng Kui Zhang Jin-Lin Miao Hong-Yong Cui Min Huang Jun Zhang Ling Fu Xiang-Min Yang Zhongpeng Zhao Shihui Sun Hongjing Gu Zhe Wang Chun-Fu Wang Yacheng Lu Ying-Ying Liu Qing-Yi Wang Huijie Bian Ping Zhu Zhi-Nan Chen | 2020 | Signal Transduction and Targeted Therapy2020，5，1： | 9 |
| 2 | Two new prenylflavonoids from Morus nigra L.显示文摘二新 prenylflavonoids, mornigrol E (1 ) 和 mornigrol F (2 ) 从桑属 nigra L 被孤立。他们的结构根据分光镜的方法被阐明,特别 2D-NMR 技术。 | Lei Wang Ting Gong Ruo Yun Chen | 2009 | Chinese Chemical Letters2009，20，12： | 8 |
| 3 | A novel impedance immunosensor based on O-phenylenediamine modified gold electrode to analyze abscisic acid显示文摘An impedance immunosensor based on O-phenylenediamine modified gold electrode for the determination of phytohormone abscisic acid(ABA) was proposed.The operating pH,absorption time,absorption temperature and concentration of anti-ABA antibody were investigated to optimize the analytical performance.The calibration curve for the determination of ABA was obtained from this impedance immunosensor under optimal conditions.The results showed that the detection limit at about 1 ng/mL in the range of 10-5000 ng... | Li, Qian Wang, Ruo Zhong Huang, Zhi Gang Li, He Song Xiao, Lang Tao | 2010 | Chinese Chemical Letters2010，21，4： | 7 |
| 4 | Visfatin Protects Rat Pancreatic β-cells against IFN-γ-Induced Apoptosis through AMPK and ERK1/2 Signaling Pathways显示文摘Objective Interferon-γ(IFN-γ) plays an important role in apoptosis and was shown to increase the risk of diabetes. Visfatin, an adipokine, has anti-diabetic, anti-tumor, and regulating inflammatory properties. In this study we investigated the effect of visfatin on IFN-γ-induced apoptosis in rat pancreatic β-cells. Methods The RINm5F(rat insulinoma cell line) cells exposed to IFN-γ were treated with or without visfatin. The viability and apoptosis of the cells were assessed by using MTT and flow cytometry. The expressions of m RNA and protein were detected by using real-time PCR and western blot analysis. Results The exposure of RINm5 F cells to IFN-γ for 48 h led to increased apoptosis percentage of the cells. Visfatin pretreatment significantly increased the cell viability and reduced the cell apoptosis induced by IFN-γ. IFN-γ-induced increase in expression of p53 m RNA and cytochrome c protein, decrease in m RNA and protein levels of anti-apoptotic protein Bcl-2 were attenuated by visfatin pretreatment. Visfatin also increased AMPK and ERK1/2 phosphorylation and the anti-apoptotic action of visfatin was attenuated by the AMPK and ERK1/2 inhibitor. Conclusion These results suggested that visfatin protected pancreatic islet cells against IFN-γ-induced apoptosis via mitochondria-dependent apoptotic pathway. The anti-apoptotic action of visfatin is mediated by activation of AMPK and ERK1/2 signaling molecules. | XIANG Ruo Lan MEI Mei SU Yun Chao LI Li WANG Jin Yu WU Li Ling | 2015 | Biomedical and Environmental Sciences2015，28，3： | 7 |
| 5 | CD147 antibody specifically and effectively inhibits infection and cytokine storm of SARS-CoV-2 and its variants delta,alpha,beta,and gamma显示文摘SARS-CoV-2 mutations contribute to increased viral transmissibility and immune escape,compromising the effectiveness of existing vaccines and neutralizing antibodies.An in-depth investigation on COVID-19 pathogenesis is urgently needed to develop a strategy against SARS-CoV-2 variants.Here,we identified CD147 as a universal receptor for SARS-CoV-2 and its variants.Meanwhile,Meplazeumab,a humanized anti-CD147 antibody,could block cellular entry of SARS-CoV-2 and its variants-alpha,beta,gamma,and delta,with inhibition rates of 68.7,75.7,52.1,52.1,and 62.3%at 60μg/ml,respectively.Furthermore,humanized CD147 transgenic mice were susceptible to SARS-CoV-2 and its two variants,alpha and beta.When infected,these mice developed exudative alveolar pneumonia,featured by immune responses involving alveoli-infiltrated macrophages,neutrophils,and lymphocytes and activation of IL-17 signaling pathway.Mechanistically,we proposed that severe COVID-19-related cytokine storm is induced by a'spike protein-CD147-CyPA signaling axis':Infection of SARS-CoV-2 through CD147 initiated the JAK-STAT pathway,which further induced expression of cyclophilin A(CyPA);CyPA reciprocally bound to CD147 and triggered MAPK pathway.Consequently,the MAPK pathway regulated the expression of cytokines and chemokines,which promoted the development of cytokine storm.Importantly,Meplazumab could effectively inhibit viral entry and inflammation caused by SARS-CoV-2 and its variants.Therefore,our findings provided a new perspective for severe COVID-19-related pathogenesis.Furthermore,the validated universal receptor for SARS-CoV-2 and its variants can be targeted for COVID-19 treatment. | Jiejie Geng Liang Chen Yufeng Yuan Ke Wang Youchun Wang Chuan Qin Guizhen Wu Ruo Chen Zheng Zhang Ding Wei Peng Du Jun Zhang Peng Lin Kui Zhang Yongqiang Deng Ke Xu Jiangning Liu Xiuxuan Sun Ting Guo Xu Yang Jiao Wu Jianli Jiang Ling Li Kun Zhang Zhe Wang Jing Zhang Qingguo Yan Hua Zhu Zhaohui Zheng Jinlin Miao Xianghui Fu Fengfan Yang Xiaochun Chen Hao Tang Yang Zhang Ying Shi Yumeng Zhu Zhuo Pei Fei Huo Xue Liang Yatao Wang Qingyi Wang Wen Xie Yirong Li Mingyan Shi Huijie Bian Ping Zhu Zhi-Nan Chen | 2021 | Signal Transduction and Targeted Therapy2021，6，10： | 6 |
| 6 | A New Isoprenylated flavonol from the Leaves of Broussonetia kazinoki显示文摘A new isoprenylated flavonol, named broussonol A (1) was isolated from the leaves of Broussonetia kazinoki Sieb. The structure of broussonol A was characterized by chemical and spectral methods. | Pei Cheng ZHANG, Si WANG, Ying Hong WANG, Ruo Yun CHEN, De Quan YU* Institute of Materia Medica, Chinese Academy of Medical Science and Peking Union Medical College, Beijing 100050 | 2001 | Chinese Chemical Letters2001，12，2： | 5 |
| 7 | 酶解乳蛋白对初生仔猪小肠组织学结构及隐窝细胞增殖的影响(英文)显示文摘INTRODUCTIONTheintestineasthemajorinterfacebetweenanorganismanditsnutritionalenvironmentplaysacriticalroleinpostnataldevelopm... | Tian Wang 1,Ruo Jun Xu 2, Yuna Qiang Zhang 3, Chong Tian Zheng 1 and Jian Cheng Zhang 4 1 Department of Animal Nutrition and Feed, Nanjing Agricultural University,210095 2 Department of Zoology, The University of Hong Kong 3 Department | 1999 | 中国组织化学与细胞化学杂志1999，8，2： | 5 |
| 8 | Amperometric Determination of lndole-3-acetic Acid Based on Platinum Nanowires and Carbon Nanotubes显示文摘Platinum nanowire (PtNW) can be grown by electrodeposition in polycarbonate membrane, with the average diameter of the nanowires about 250 nm. The PtNW and multiwalled carbon nanotubes (CNT) are then dispersed into chitosan (CHIT) solution. The resulting PtNW-CNT-CHIT material brings new capabilities for electrochemical devices by using the synergistic action of the electrocatalytic activity of PtNW and CNT. By dropping the PtNW-CNT-CHIT film onto the glassy carbon (GC) electrode surface, and after evaporation an amperometric sensor for the determination of indole-3-acetic acid (IAA) was developed. The oxidation current of IAA increased significantly at the PtNW-CNT-CHIT film coated GC electrode, in contrast to that at the CNT-CHIT modified GC. The linear response of the sensor is from 50 ng/ml to 50 μg/ml with a detection limit of 25 ng/mL. | Ruo Zhong WANG Lang Tao XIAO Ming Hui YANG Jun Hui DING Feng Li QU Guo Li-SHEN | 2006 | Chinese Chemical Letters2006，17，12： | 3 |
| 9 | Precise editing of methylated cytosine in Arabidopsis thaliana using a human APOBEC3Bctd-Cas9 fusion显示文摘Dear Editor,Precise editing of specific genomic sites holds great promise in basic research and modern crop breeding,and some cytosine base editors(CBEs)have been developed for this purpose(Komor et al.,2016;Zong et al.,2018). | Zhi-Hong Liu Shanjie Tang Wentao Hu Ruo Lv Hailiang Mei Rongxin Yang Xianwei Song Xiaofeng Cao Dong Wang | 2022 | Science China(Life Sciences)2022，65，1： | 3 |
| 10 | Safety and efficacy of meplazumab in healthy volunteers and COVID-19 patients: a randomized phase 1 and an exploratory phase 2 trial显示文摘Recent evidence suggests that CD147 serves as a novel receptor for severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)infection.Blocking CD147 via anti-CD147 antibody could suppress the in vitro SARS-CoV-2 replication.Meplazumab is a humanized anti-CD147 IgG_(2) monoclonal antibody,which may effectively prevent SARS-CoV-2 infection in coronavirus disease 2019(COVID-19)patients.Here,we conducted a randomized,double-blinded,placebo-controlled phase 1 trial to evaluate the safety,tolerability,and pharmacokinetics of meplazumab in healthy subjects,and an open-labeled,concurrent controlled add-on exploratory phase 2 study to determine the efficacy in COVID-19 patients.In phase 1 study,59 subjects were enrolled and assigned to eight cohorts,and no serious treatment-emergent adverse event(TEAE)or TEAE grade≥3 was observed.The serum and peripheral blood Cmax and area under the curve showed non-linear pharmacokinetic characteristics.No obvious relation between the incidence or titer of positive anti-drug antibody and dosage was observed in each cohort.The biodistribution study indicated that meplazumab reached lung tissue and maintained>14 days stable with the lung tissue/cardiac blood-pool ratio ranging from 0.41 to 0.32.In the exploratory phase 2 study,17 COVID-19 patients were enrolled,and 11 hospitalized patients were involved as concurrent control.The meplazumab treatment significantly improved the discharged(P=0.005)and case severity(P=0.021),and reduced the time to virus negative(P=0.045)in comparison to the control group.These results show a sound safety and tolerance of meplazumab in healthy volunteers and suggest that meplazumab could accelerate the recovery of patients from COVID-19 pneumonia with a favorable safety profile. | Huijie Bian Zhao-Hui Zheng Ding Wei Aidong Wen Zheng Zhang Jian-Qi Lian Wen-Zhen Kang Chun-Qiu Hao Jing Wang Rong-Hua Xie Ke Dong Jie-Lai Xia Jin-Lin Miao Wen Kang Guoquan Li Di Zhang Mingru Zhang Xiu-Xuan Sun Likun Ding Kui Zhang Junfeng Jia Jin Ding Zhiqin Li Yanyan Jia Lin-Na Liu Zhe Zhang Zhao-Wei Gao Hong Du Na Yao Qing Wang Ke Wang Jie-Jie Geng Bin Wang Ting Guo Ruo Chen Yu-Meng Zhu Li-Juan Wang Qian He Rui-Rui Yao Ying Shi Xiang-Min Yang Jian-Sheng Zhou Yi-Nan Ma Ya-Tao Wang Xue Liang Fei Huo Zhe Wang Yang Zhang Xu Yang Ye Zhang Lu-Hua Gao Ling Wang Xiao-Chun Chen Hao Tang Shuang-Shuang Liu Qing-Yi Wang Zhi-Nan Chen Ping Zhu | 2021 | Signal Transduction and Targeted Therapy2021，6，6： | 2 |
| 11 | Benzofuran-isatin-hydroxylimine/thiosemicarbazide hybrids:Design,synthesis and in vitro anti-mycobacterial activity evaluation显示文摘A series of novel benzofuran-isatin-hydroxylimine/thiosemicarbazide hybrids were designed, synthesized and evaluated for their in vitro anti-TB activities against drug-sensitive MTB H_(37)Rv and MDR-TB isolates as well as cytotoxicity. All benzofuran-isatin-hydroxylimine/thiosemicarbazide hybrids exhibited considerable in vitro anti-mycobacterial activities against the tested three MTB strains, and all of them also showed acceptable cytotoxicity. The most active hybrid 7f was >4.8 and >51 folds more potent than the first line anti-TB agents RIF and INH against both drug-sensitive MTB H_(37)Rv and MDR-TB isolates, respectively. The results demonstrated the potential utility of benzofuran-isatin-hydroxylimine/-thiosemicarbazide hybrids as anti-TB agents. | Yaohuan Zhang Ruo Wang Tesen Zhang Weitao Yan Yihong Chen Yanping Zhang Muyang Zhou | 2019 | Chinese Chemical Letters2019，30，3： | 2 |
| 12 | Alkaloids from the Roots of Saccopetalum prolificum显示文摘A new, alkaloid. named prolilfine (1), was isolated along with four known alkaloids. liriodenine (2). 6-hydroxyonychine (3). isooncodine (4) and discretamine (5) from the roots of Saccopetalum prolificum. The structure of I was elucidated on the basis of spectroscopic and chemical methods. | Ming Lei WANG Jiang DU Ruo Yun CHEN De Quan YU (Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050) | 2000 | Chinese Chemical Letters2000，11，2： | 2 |
| 13 | A Multigrid Block LU-SGS Algorithm for Euler Equations on Unstructured Grids显示文摘We propose an efficient and robust algorithm to solve the steady Euler equa- tions on unstructured grids.The new algorithm is a Newton-iteration method in which each iteration step is a linear multigrid method using block lower-upper symmetric Gauss-Seidel(LU-SGS)iteration as its smoother To regularize the Jacobian matrix of Newton-iteration,we adopted a local residual dependent regularization as the replace- ment of the standard time-stepping relaxation technique based on the local CFL number The proposed method can be extended to high order approximations and three spatial dimensions in a nature way.The solver was tested on a sequence of benchmark prob- lems on both quasi-uniform and local adaptive meshes.The numerical results illustrated the efficiency and robustness of our algorithm. | Ruo Li Xin Wang Weibo Zhao | 2008 | Numerical Mathematics(Theory,Methods and Applications)2008，1，1： | 2 |
| 14 | Electrocatalytic Oxidation of NADH Based on Self-assembled Colloidal Gold and Nation Matrixes and Co Complex Mediator显示文摘A novel approach based on self-assembled colloidal gold and Nafion matrixes and Co complex mediator to construct Co(bpy)33+/nano-Au/Co(bpy)33+/nafion/GC electrode, on which formed stable redox-active films. This electrode can decrease the overpotential about 330 mV for the oxidation of NADH with high stability, wide linear range and low detection limit. | Na WANG Ruo YUAN Ya Qin CHAI Dian Ping TANG Qiang ZHU Xue Lian LI | 2006 | Chinese Chemical Letters2006，17，5： | 2 |
| 15 | Characteristics of the Cochlear Symptoms and Functions in Meniere's Disease显示文摘 | Yi Zhang Bo Liu Rui Wang Ruo Jia Xin Gu | 2016 | Chinese Medical Journal2016，，20： | 2 |
| 16 | 显示文摘 | Wang Yan Yuan Ruo Chai Yaqin | 2011 | Biosens Bioelec- tron2011，30，1： | 1 |
| 17 | Identification and molecular characterization of a novel antigen of Eimer- ia acervulina显示文摘 | ZHU Hui - li YAN Ruo - feng WANG Song | 2012 | Molecular and Biochemical Parasitology2012，186，1： | 1 |
| 18 | Transvaginal surgical treatment of cesarean scar ectopic pregnancy显示文摘 | Aiwen Le Lili Shan Tianhui Xiao Ruo Zhuo Huijian Xiong Zhonghai Wang | 2013 | Archives of Gynecology and Obstetrics2013，，4： | 1 |
| 19 | Amperometric third-generation hydrogen peroxide biosensor based on the immobilization of hemoglobin on multiwall carbon nanotubes and gold colloidal nanoparticles显示文摘 | Shihong Chen Ruo Yuan Yaqin Chai Lingyan Zhang Na Wang Xuelian Li | 2006 | Biosensors and Bioelectronics2006，，7： | 1 |
| 20 | Highly selective thiocyanate electrode based on bis-bebzoin-semitriethylene tetraamine binuclear copper( Ⅱ ) complex as neutral carrier显示文摘 | YUAN Ruo WANG Xiu-ling CHAI Ya-qin | 2003 | Electrochem Commun2003，5，8： | 1 |
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