|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Cryo-copolymerization preparation of dextran-hyaluronate based supermacroporous cryogel scaffolds for tissue engineering applications显示文摘Dextran-hyaluronate (Dex -- 哈) 为软织物工程的基于的 supermacroporous cryogel 脚手架被在结冰条件下面在各种各样的 macromonomer 集中包含葡聚糖 methacrylate (Dex 麻省) 和 hyaluronate methacrylate (哈麻省) 的水的答案的免费激进的 cryo-copolymerization 准备。这被观察为 Dex 的准备的 macromonomers 的合适的全部的集中 -- 哈有满足的性质的 cryogel 支架是 5%(w/w ) 在 1% 的哈麻省集中(w/v ) ,它然后被用来生产测试支架。有 5% 的获得的 cryogel 支架(w/w ) macromonomer 答案有高水位线渗透(5.1 ? 桴 ? 潨灳瑩? | Dongjiao ZHOU Shaochuan SHEN Junxian YUN Kejian YAO Dong-Qiang LIN | 2012 | Frontiers of Chemical Science and Engineering2012，6，3： | 1 |
| 2 | The identification of Pi50(t), a new member of the rice blast resistance Pi2/Pi9 multigene family显示文摘 | Xiaoyuan Zhu Shen Chen Jianyuan Yang Shaochuan Zhou Liexian Zeng Jingluan Han Jing Su Ling Wang Qinghua Pan | 2012 | Theoretical and Applied Genetics2012，，7： | 1 |
| 3 | Coal Seam Methane Distribution and Its Significance in Pingdingshan Mining Area 显示文摘 | GUO Deyong ZHOU Xinquan SHEN Shaochuan | 2002 | Journal of Coal Science & Engineering2002，8，2： | 1 |
| 4 | The identification of Pi50(t), a new member of the rice blast resistance Pi2/Pi9 multigene family显示文摘 | Xiaoyuan Zhu Shen Chen Jianyuan Yang Shaochuan Zhou Liexian Zeng Jingluan Han Jing Su Ling Wang Qinghua Pan | 2012 | Theoretical and Applied Genetics2012，，7： | 1 |
| 5 | Coal forming environments and their relationship to tectonic activity in the Cevennes Stephanian coal basin显示文摘 | Guo Deyong Zhou Xinquan Shen Shaochuan | 2002 | Journal of coal Science & Engineering ( China )2002，8，： | 1 |
| 6 | Chromatographic Adsorption of Serum Albumin and Antibody Proteins in Cryogels with Benzyl-Quaternary Amine Ligands显示文摘 | Junxian Yun Xiuhong Cheng Jialei Ye Shaochuan Shen Gensheng Yang Kejian Yao Harald Kirsebom Dong-Qiang Lin Yi-Xin Guan Shan-Jing Yao | 2014 | Journal of Chromatography A2014，，： | 1 |
| 7 | Separation of Recombinant Geranylgeranyl Diphosphate Synthase of Deinococcus radiodurans from Expressed Strain Cell Homogenate by Immobilized Metal Affinity Chromatography on a Characterized Monolithic Cryogel Column显示文摘Geranylgeranyl diphosphate synthase (GGPPS) plays a key role in the biosynthesis of antioxidative ca-rotenoid from the extremely radioresistant bacterium Deinococcus radiodurans. In this work, the recombinant GGPPS expressed in Escherichia coli by cloning and transforming the gene dr1395 of D. radiodurans was isolated rapidly by an immobilized metal affinity supermacroporous cryogel, i.e., Cu2+-iminodiacetic acid (IDA)-cryogel. The properties of the Cu2+-IDA-cryogel were characterized using capillary-based mathematical model and experi-mental measurements. The obtained protein samples were analyzed by the sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The porosity of the present Cu2+-IDA-cryogel is 90.4% and the water permeabil-ity is 5.04×10-12 m2. From the capillary-based model, this cryogel presents a slightly wide normal pore (capillary) size distribution with the mean diameter of 55.2 μm, the standard deviation of 28.0 μm and the half of skeleton wall thickness of 2.8 μm. The pore size distribute from about 10 to 141 μm and the effective tortuosity of these capillary pores increases from 2.60 to 9.05. The isolation of the GGPPS from cell homogenate can be achieved at the flow velocity of 3.40×10-4 m·s-1 by the Cu2+-IDA-cryogel bed. High-purity GGPPS (about 91.4%) is obtained according to the SDS-PAGE analysis of the elution samples, indicating that the present method is a promising, simple and ef-fective approach to isolate GGPPS from cell homogenate of engineering strains. | SHEN Shaochuan WANG Liangyan SUN Zongtao LI Mingfeng LIU Chengzhi TIAN Bing YUN Junxian HUA Yuejin | 2013 | Chinese Journal of Chemical Engineering2013，21，6： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员