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| 1 | Structure-function relationship of the mammarenavirus envelope glycoprotein显示文摘Mammarenaviruses, including lethal pathogens such as Lassa virus and Junín virus, can cause severe hemorrhagic fever in humans. Entry is a key step for virus infection, which starts with binding of the envelope glycoprotein(GP) to receptors on target cells and subsequent fusion of the virus with target cell membranes. The GP precursor is synthesized as a polypeptide, and maturation occurs by two cleavage events, yielding a tripartite GP complex(GPC) formed by a stable signal peptide(SSP), GP1 and GP2. The unique retained SSP interacts with GP2 and plays essential roles in virion maturation and infectivity. GP1 is responsible for binding to the cell receptor, and GP2 is a class I fusion protein. The native structure of the tripartite GPC is unknown.GPC is critical for the receptor binding, membrane fusion and neutralization antibody recognition.Elucidating the molecular mechanisms underlining the structure–function relationship of the three subunits is the key for understanding their function and can facilitate novel avenues for combating virus infections. This review summarizes the basic aspects and recent research of the structure–function relationship of the three subunits. We discuss the structural basis of the receptor-binding domain in GP1, the interaction between SSP and GP2 and its role in virion maturation and membrane fusion, as well as the mechanism by which glycosylation stabilizes the GPC structure and facilitates immune evasion. Understanding the molecular mechanisms involved in these aspects will contribute to the development of novel vaccines and treatment strategies against mammarenaviruses infection. | Wei Wang Zheng Zhou Leike Zhang Shaobo Wang Gengfu Xiao | 2016 | Virologica Sinica2016，31，5： | 4 |
| 2 | Cellular membrane cholesterol is required for porcine reproductive and respiratory syndrome virus entry and release in MARC-145 cells显示文摘Cholesterol represents one of the key constituents of small,dynamic,sterol-and sphingolipid-enriched domains on the plasma membrane.It has been reported that many viruses depend on plasma membrane cholesterol for efficient infection.In this study,the role of the plasma membrane cholesterol in porcine reproductive and respiratory syndrome virus(PRRSV) infection of MARC-145 cells was investigated.Pretreatment of MARC-145 cells with methyl-β-cyclodextrin(MβCD),a drug used to deplete cholesterol from cellular membrane,significantly reduced PRRSV infection in a dose-dependent manner.This inhibition was partially reversed by supplementing exogenous cholesterol following MβCD treatment,suggesting that the inhibition of PRRSV infection was specifically mediated by removal of cellular cholesterol.Further detailed studies showed that depletion of cellular membrane cholesterol significantly inhibited virus entry,especially virus attachment and release.These results indicate that the presence of cholesterol in the cellular membrane is a key component of PRRSV infection. | SUN Ying XIAO ShaoBo WANG Dang LUO Rui LI Bin CHEN HuanChun FANG LiuRong | 2011 | Science China(Life Sciences)2011，54，11： | 3 |
| 3 | Porcine reproductive and respiratory syndrome virus (PRRSV) suppresses interferon-β production by interfering with the RIG-I signaling pathway显示文摘 | Rui Luo Shaobo Xiao Yunbo Jiang Hui Jin Dang Wang Manli Liu Huanchun Chen Liurong Fang | 2008 | Molecular Immunology2008，，10： | 2 |
| 4 | C3d enhanced DNA vaccination induced humoral immune response to glycoprotein C of pseudorabies virus显示文摘 | Tiezhu Tong Huiying Fan Yadi Tan Shaobo Xiao Jieyu Ling Huanchun Chen Aizhen Guo | 2006 | Biochemical and Biophysical Research Communications2006，，4： | 1 |
| 5 | Influence of Nano-Al_2O_3 on Properties of Magnesia Chrome Refractories显示文摘Magnesia chrome bricks were prepared with fused MgO-Cr2O3 synthesized material,fused magnesite and chrome ore as main starting materials. Nano-Al2O3 was added into refractories ( 2% ,4% and 6% in mass) substituting for Al2O3 micropowder. After mixing and shaping,the bricks were fired at 1 550 ℃ ,1 600 ℃ , 1 650 ℃ and 1 750 ℃ ,respectively. The microstruc-ture,sintering property,mechanical properties,thermal shock resistance and slag resistance of the specimens with the addition of nano-Al2O3 were investigated. The re-sults indicate that the performance of brick with 4 mass% of nano-Al2O3 is greatly improved after firing at 1 650 ℃ . | ZHANG Daoyun IIANG Mingxue XIAO Guoqing HUANG Shaobo | 2012 | China's Refractories2012，21，2： | 1 |
| 6 | Antiviral activity of type I and type III interferons against porcine reproductive and respiratory syndrome virus (PRRSV)显示文摘 | Rui Luo Liurong Fang Hui Jin Yunbo Jiang Dang Wang Huanchun Chen Shaobo Xiao | 2011 | Antiviral Research2011，，2： | 1 |
| 7 | Foot-and-mouth disease virus (FMDV) leader proteinase negatively regulates the porcine interferon-λ1 pathway显示文摘 | Dang Wang Liurong Fang Lizhi Liu Huijuan Zhong Quangang Chen Rui Luo Xiangtao Liu Zhongming Zhang Huanchun Chen Shaobo Xiao | 2011 | Molecular Immunology2011，，1： | 1 |
| 8 | Porcine Reproductive and Respiratory Syndrome Virus Induces IL-1 β Production Depending on TLR4/MyD88 Pathway and NLRP3 Inflammasome in Primary Porcine Alveolar Macrophages显示文摘 | Jing Bi Shuang Song Liurong Fang Dang Wang Huiyuan Jing Li Gao Yidong Cai Rui Luo Huanchun Chen Shaobo Xiao Vera L. Petricevich | 2014 | Mediators of Inflammation2014，，： | 1 |
| 9 | Molecular cloning, expression and antiviral activity of porcine interleukin-29 (poIL-29)显示文摘 | Dang Wang Liurong Fang Fuwei Zhao Rui Luo Huanchun Chen Shaobo Xiao | 2010 | Developmental and Comparative Immunology2010，，3： | 1 |
| 10 | Induction of autophagy enhances porcine reproductive and respiratory syndrome virus replication显示文摘 | Quangang Chen Liurong Fang Dang Wang Shaohua Wang Ping Li Mao Li Rui Luo Huanchun Chen Shaobo Xiao | 2011 | Virus Research2011，，2： | 1 |
| 11 | Immunogenicity and Protective Efficacy of Recombinant Pseudorabies Virus Expressing the Two Major Membrane - Associated Proteins of porcine Reproductive and Respiratory Syndrome Virus显示文摘 | Jiang Yunbo Fang Liurong Xiao Shaobo | 2007 | Vaccine2007，，25： | 1 |
| 12 | Porcine reproductive and respiratory syndrome virus infection activates IL-10 production through NF κB and p38 MAPK pathways in porcine alveolar macrophages显示文摘 | Shaobo Xiao Shuang Song Jing Bi | 2013 | Developmental and Comparative Immunology2013，39，： | 1 |
| 13 | Intervention Effects of Ganoderma Lueidum Spores on Epileptiform Discharge Hippocampal Neurons and Expression of Neurotrophin- 4 and N - Cadherln显示文摘 | Shu - Qiu Wang Xiao - Jie Li Shaobo Zhou | 2013 | Plos one2013，8，61： | 1 |
| 14 | Improvement of in situ LA-ICP-MS U-Pb dating method for carbonate minerals and its application in petroleum geology显示文摘In situ carbonate U-Pb dating is gaining popularity,and it has great potential for application in petroleum geology.However,the low U content(<10μg/g)and high common Pb content of carbonate minerals,along with the uneven distribution of U and Pb and the lack of matrix-matched reference material make carbonate U-Pb dating inaccurate and less successful,which limits the widespread application in geosciences.This study evaluated the limitations of in situ carbonate U-Pb dating and proposed a method to rationally determine the laser ablation parameters of samples by improving the experimental approach based on the laser ablation sector field inductively coupled plasma mass spectrometry(LA-SF-ICP-MS).By setting a different spot size and laser frequency for the reference material and unknown samples in the same session based on the U content of the sample,the ablation craters of the reference material and unknown samples were given the same depth/width ratio,avoiding systematic offset caused by differences in down-hole element fractionation and reducing the consume of reference material.Depending on the heterogeneous distribution of U and Pb contents in carbonate minerals,the method of grid screening and setting ablation spots during screening were used to quickly select domains with high U and low common Pb,which improves the efficiency of setting laser spots and the success rate of dating,as well as reduces the experimental time and economic cost.The accuracy and success rate of carbonate U-Pb dating were effectively improved by improving the experimental method,and the technique was applied to two carbonate samples with low U and high common Pb contents that were difficult to date by traditional methods.The two samples are the saddle dolomite in the central Sichuan Basin and the calcite cement in the sandstone reservoir of the Cretaceous Qingshuihe Formation in the South Junggar Basin.The robust ages have been obtained,which constrains the timing of the diagenetic and hydrocarbon accumulation process in the studied area. | Xuesong Lu Lili GUI Weiyan CHEN Shaobo LIU Shitou WU Junjia FAN Qiang LIU Jing SUN Liangliang ZHANG Yang XIAO Wenxia YANG Rizhou CAO | 2023 | Science China Earth Sciences2023，66，12： | 1 |
| 15 | Hypodermin A, a potential agent for prevention of allogeneic acute rejection显示文摘 | Quangang Chen Renjin Chen Jing Liu Honghua Yuan Peng Liu Ankang Hu Lianlian Wu Liurong Fang Shaobo Xiao Xiaorong Zhu | 2015 | Transplant Immunology2015，，3： | 1 |
| 16 | DNA vaccines co-expressing GP5 and M proteins of porcine reproductive and respiratory syndrome virus (PRRSV) display enhanced immunogenieity显示文摘 | Jiang Yunbo Xiao Shaobo Fang Liurong | 2006 | Vaccine2006，24，： | 1 |
| 17 | Porcine reproductive and respiratory syndrome virus (PRRSV) infection activates chemokine RANTES in MARC-145 cells显示文摘 | Yanwei Wang Rui Luo Liurong Fang Dang Wang Jing Bi Huanchun Chen Shaobo Xiao | 2010 | Molecular Immunology2010，，4： | 1 |
| 18 | Porcine reproductive and respiratory syndrome virus infection activates NOD2–RIP2 signal pathway in MARC-145 cells显示文摘 | Huiyuan Jing Liurong Fang Dang Wang Zhen Ding Rui Luo Huanchun Chen Shaobo Xiao | 2014 | Virology2014，，： | 1 |
| 19 | Herpes simplex virus VP22 enhances adenovirus-mediated microdystrophin gene transfer to skeletal muscles in dystrophin-deficient (mdx) mice 显示文摘 | Xiong Fu Xiao Shaobo Peng Funing | 2007 | Hum Gene Ther2007，18，6： | 1 |
| 20 | Construction and characterization of a live, attenuated apxIICA inactivation mutant of Actinobacillus pleuropneumoniae lacking a drug resistance marker显示文摘 | Weicheng Bei Qigai He Lin Yan Liurong Fang Yadi Tan Shaobo Xiao Rui Zhou Meilin Jin Aizhen Guo Jianqiang Lv Hongliang Huang Huanchun Chen | 2004 | FEMS Microbiology Letters2004，，1： | 1 |
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