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| 1 | Electrochemical single nucleotide polymorphisms genotyping on surface immobilized three-dimensional branched DNA nanostructure显示文摘An electrochemical assay for single nucleotide polymorphisms (SNPs) genotyping is reported. Although electrochemical method is sensitive for DNA detection on surfaces, the ability of surface assay to precisely recognize DNA hybridization event is sacrificed to some extent due to the crowded confined surfaces environments that disfavor DNA hybridization. In the present study, we employed branched tetrahedron structure probes (TSPs) to replace regular linear single stranded DNA capture probes that were immobilized on solid surfaces. This three-dimensional DNA nanostructure lowers the density of immobilized DNA probes on confined surfaces, providing a hybridization environment that is similar to homogenous solution. This TSP-based electrochemical assay reveals excellent performance for SNPs genotyping with concentration as low as 1 nM. | GE ZhiLei PEI Hao WANG LiHua SONG ShiPing FAN ChunHai | 2011 | Science China Chemistry2011，54，8： | 13 |
| 2 | Production of β-1,3-glucanase and chitinase of two biocon-trol agents and their possible modes of action显示文摘Pichia membranefaciens Hansen and Candida guilliermondii (Cast) Langeronet Guerra are two antagonists of R. stolonifer on harvested nectarine and peach fruits. In this study, β-1,3-glucanase and chitinase activities of the antagonists were induced in vitro and in vivo. The highest β-1, 3-glucanase activity was detected in Lilly-Barnett minimal salt medium supplemented with glucose in combination with CWP of R. stolonifer as a carbon source. The β-1,3-glucanase activity of P. membranefaciens reached the maximum level, being 114.0 SU (specific activity unit), and that of C. guilliermondii reached 103.2 SU. The lowest β-1,3-glucanase activity was observed in the medium containing glucose as sole car-bon source. P. membranefaciens was able to produce signifi-cantly higher levels of chitinase (exochitinase and endochiti-nase) in vitro than C. guilliermondii grown in Czapeck mini-mal medium. An increase in β-1,3-glucanase and chitinase activity was also triggered by wounding, adding of carbon sources | FAN Qing, TIAN Shiping, LIU Haibo & XU YongInstitute of Botany, Chinese Academy of Sciences, Beijing 100093, China | 2002 | Chinese Science Bulletin2002，47，4： | 11 |
| 3 | Design of a Carbon Nanotube/Magnetic Nanoparticle-Based Peroxidase-Like Nanocomplex and Its Application for Highly Efficient Catalytic Oxidation of Phenols显示文摘We report a novel nanotechnology-based approach for the highly efficient catalytic oxidation of phenols and their removal from wastewater.We use a nanocomplex made of multi-walled carbon nanotubes(MWNTs)and magnetic nanoparticles(MNPs).This nanocomplex retains the magnetic properties of individual MNPs and can be effectively separated under an external magnetic fi eld.More importantly,the formation of the nanocomplex enhances the intrinsic peroxidase-like activity of the MNPs that can catalyze the reduction of hydrogen peroxide(H2O2).Significantly,in the presence of H2O2,this nanocomplex catalyzes the oxidation of phenols with high effi ciency,generating insoluble polyaromatic products that can be readily separated from water. | Xiaolei Zuo Cheng Peng Qing Huang Shiping Song Lihua Wang Di Li Chunhai Fan | 2009 | Nano Research2009，2，8： | 8 |
| 4 | Bimetallic nano-mushrooms with DNA-mediated interior nanogaps for high-efficiency SERS signal amplification显示文摘有为散布的提高表面的拉曼(重量的单位) 分析的 ultrasensitive 的明确的 nanogaps 抓住伟人诺言的一致包含银的金属 nanostructures。不过,有强壮、稳定的重量的单位的如此的 nanostructures 的直接合成表明极其质问的遗体。这里,我们与内部 nanogaps 为金银的 nano 蘑菇的直接合成报导一条调停 DNA 的途径。这些 nano 蘑菇的重量的单位紧张极其依赖于在金头和银帽子之间的 nanogap 的区域。我们发现 nanogaps 的形成由控制在金 nanoparticles 上标记搁浅单人赛的 DNA (ssDNA ) 的 6-carboxy-X-rhodamine (ROX ) 的表面密度是细微地悦耳的。我们与 1.0 的一个高重量的单位信号改进因素在高产量获得了 nano 蘑菇我们作为一项 retrofit 措施在 CHS 在用阶段变化材料(脉冲编码调制) 的热存储的采纳以后。结果显示那与融化 23 的温度使用脉冲编码调制 ? 訄吗?? | Jianlei Shen Jing Su Juan Yan Bin Zhao Dongfang Wang Siyi Wang Kun Li Mengmeng Liu Yao He Sanjay Mathur Chunhai Fan Shiping Song | 2015 | Nano Research2015，8，3： | 4 |
| 5 | A nanoresonant gold-aptamer probe for rapid and sensitive detection of thrombin显示文摘Resonance light scattering (RLS) is a sensitive technique for monitoring scattered light induced by extended aggregates of chromophores. It has been widely used to study aggregations for its simple manipulation, high sensitivity and great versatility. Gold nanoparticles generate colorful light-scattering signals due to their unique surface plasmon resonances, hence extraordinary light scattering upon aggregation. In this paper we report a rapid and sensitive method based on gold nanoparticles and DNA aptamer to detect protein biomarkers by RLS. Thiol modified thrombin aptamer was covalently assembled to the surface of gold nanoparticles as nanobio probes. As thrombin has two specific binding sites for its aptamer, it can bridge the well dispersed nanoparticles and lead to a network of particle aggregations. The formation of aggregation ia measured by RLS, and the specific detection of thrombin at nM level is achieved. The method has good specificity. | ZHENG Xiaoxue WEN Yanqin ZHANG Juan WANG Lihua SONG Shiping ZHANG Hua FAN Chunhai | 2012 | Nuclear Science and Techniques2012，23，5： | 4 |
| 6 | A Nano-and Micro-Integrated Protein Chip Based on Quantum Dot Probes and a Microfluidic Network显示文摘A novel nano-and micro-integrated protein chip(NMIPC)that can detect proteins with ultrahigh sensitivity has been fabricated.A microfl uidic network(μFN)was used to construct the protein chips,which allowed facile patterning of proteins and subsequent biomolecular recognition.Aqueous phase-synthesized,water-soluble fl uorescent CdTe/CdS core-shell quantum dots(aqQDs),having high quantum yield and high photostability,were used as the signaling probe.Importantly,it was found that aqQDs were compatible with microfluidic format assays,which afforded highly sensitive protein chips for cancer biomarker assays. | Juan Yan Mei Hu Di Li Yao He Rui Zhao Xingyu Jiang Shiping Song Lianhui Wang Chunhai Fan | 2008 | Nano Research2008，1，6： | 3 |
| 7 | Characterization of a New Gene wx2 in Toxoplasma gondii显示文摘用杂交技术,我们准备了单音的同种细胞的抗体(Mab ) 对 Toxoplasma gondii 的 7C3-C3。保护测试证明蛋白质(Mab7C3-C3 ) 禁止了 T 的侵略和增长。在 HeLa 房间的 gondii RH 紧张。被动转移测试显示抗体显著地延长了质问老鼠的幸存时间。抗体能被用于 T.gondii 的传播抗原的察觉,这也被显示出。在屏蔽 T 的免疫以后。有 Mab7C3-C3 的 gondii tachyzoite cDNA 图书馆,新基因 wx2of T。gondii 被获得。Immunofluorescence 分析证明 WX2 蛋白质是寄生虫的定位的在膜。核苷酸顺序比较显示出 28% 身份到钙隧道 alpha-1E 单位并且与表面抗原分享了在一些保守残余的相关顺序。然而,没有火柴在蛋白质数据库被发现。因此,它是在在 T 的膜上编码功能的蛋白质的 T.gondii 的未知基因。gondii。因为对 T 有部分保护的效果被显示了。gondii 感染并且作为传播抗原被释放,它能为新药是为疫苗或一个新奇目标的一个候选人分子。 | Xiang WU Qiong ZHANG Kui TAN Ronghua XIE Jiubo FAN Hengping SHU Shiping WANG | 2007 | Acta Biochimica et Biophysica Sinica2007，39，7： | 2 |
| 8 | The enzyme-amplified amperometric DNA sensor using an electrodeposited polymer redox mediator显示文摘A highly sensitive method for the detection of a breast cancer-associated BRCA-1 gene is reported. The detection is based on a classical sandwich-type assay using horseradish peroxidase (HRP) as a catalytic label and electrodeposited Os2+/3+ conducting polymer (PAA-PVI-Os) as a redox mediator. Target DNA could be detected by the HRP-catalyzed reduction of H2O2, leading to a limit of detection as low as 10 fM. | ZHANG LanYong WAN Ying ZHANG Jiong LI Di WANG LiHua SONG ShiPing FAN ChunHai | 2009 | Science China Chemistry2009，52，6： | 2 |
| 9 | An Enhanced Monte Carlo Localization Algorithm for Mobile Node in Wireless Sensor Networks 显示文摘 | Shiping Fan Yongiang Wen Lin Zhou | 2013 | Applied Mechanics and Ma-terials2013，，： | 1 |
| 10 | Post-harvest biologicalcontrol of gray mould and blue mould on apple byCryptococcus albodus(Saito)Skinner显示文摘 | FAN QING TIAN SHIPING | | 0，，： | 1 |
| 11 | Matroidal structure of rough sets and its characterization to attribute reduction显示文摘 | Shiping Wang Qingxin Zhu William Zhu Fan Min | 2012 | Knowledge-Based Systems2012，，： | 1 |
| 12 | Preparation of nanocrystalline ceramic oxide powders in the presence of anionic starburst dendrimer显示文摘 | Zhang Fan Yang Shiping Wang Weiming | 2004 | Mater Lett2004，58，26： | 1 |
| 13 | An invasive zone in human liver cancer identified by Stereo-seq promotes hepatocyte–tumor cell crosstalk,local immunosuppression and tumor progression显示文摘Dissecting and understanding the cancer ecosystem,especially that around the tumor margins,which have strong implications for tumor cell infiltration and invasion,are essential for exploring the mechanisms of tumor metastasis and developing effective new treatments.Using a novel tumor border scanning and digitization model enabled by nanoscale resolution-SpaTial Enhanced REsolution Omics-sequencing(Stereo-seq),we identified a 500µm-wide zone centered around the tumor border in patients with liver cancer,referred to as“the invasive zone”.We detected strong immunosuppression,metabolic reprogramming,and severely damaged hepatocytes in this zone.We also identified a subpopulation of damaged hepatocytes with increased expression of serum amyloid A1 and A2(referred to collectively as SAAs)located close to the border on the paratumor side.Overexpression of CXCL6 in adjacent malignant cells could induce activation of the JAK-STAT3 pathway in nearby hepatocytes,which subsequently caused SAAs’overexpression in these hepatocytes.Furthermore,overexpression and secretion of SAAs by hepatocytes in the invasive zone could lead to the recruitment of macrophages and M2 polarization,further promoting local immunosuppression,potentially resulting in tumor progression.Clinical association analysis in additional five independent cohorts of patients with primary and secondary liver cancer(n=423)showed that patients with overexpression of SAAs in the invasive zone had a worse prognosis.Further in vivo experiments using mouse liver tumor models in situ confirmed that the knockdown of genes encoding SAAs in hepatocytes decreased macrophage accumulation around the tumor border and delayed tumor growth.The identification and characterization of a novel invasive zone in human cancer patients not only add an important layer of understanding regarding the mechanisms of tumor invasion and metastasis,but may also pave the way for developing novel therapeutic strategies for advanced liver cancer and other solid tumors. | Liang Wu Jiayan Yan Yinqi Bai Feiyu Chen Xuanxuan Zou Jiangshan Xu Ao Huang Liangzhen Hou Yu Zhong Zehua Jing Qichao Yu Xiaorui Zhou Zhifeng Jiang Chunqing Wang Mengnan Cheng Yuan Ji Yingyong Hou Rongkui Luo Qinqin Li Liang Wu Jianwen Cheng Pengxiang Wang Dezhen Guo Waidong Huang Junjie Lei Shang Liu Yizhen Yan Yiling Chen Sha Liao Yuxiang Li Haixiang Sun Na Yao Xiangyu Zhang Shiyu Zhang Xi Chen Yang Yu Yao Li Fengming Liu Zheng Wang Shaolai Zhou Huanming Yang Shuang Yang Xun Xu Longqi Liu Qiang Gao Zhaoyou Tang Xiangdong Wang Jian Wang Jia Fan Shiping Liu Xinrong Yang Ao Chen Jian Zhou | 2023 | Cell Research2023，33，8： | 1 |
| 14 | Prognosis of gestational choriocarcinoma diagnosed incidentally during laparoscopy for a presumed cornual pregnancy:a report of five cases显示文摘The clinical presentations of gestational choriocarcinoma vary markedly, and a misdiagnosis could be made in atypical patients if simply relying on clinical features. Laparoscopic resection of uterine mass lesion is rarely used in gestational choriocarcinoma diagnosis because of the fear of heavy blood loss and distant metastasis. Five patients who were preoperatively diagnosed as having cornual pregnancy underwent laparoscopic resection of mass lesion and then proved to have gestational choriocarcinoma based on pathological examinations. Chemotherapy was started within two days after surgery, and the rate of complete remission was 100%. The mean follow-up time was 29.8±19.1 months, and no patient showed signs of relapse. Laparoscopic resection of uterine mass followed by timely postoperative chemotherapy may be an effective and safe way to obtain pathologic results in patients with suspected gestational choriocarcinoma. | Shiping Liu Chenchen Fan Fengzhi Feng Yang Xiang Xirun Wan Tong Ren | 2017 | Cancer Biology & Medicine2017，14，2： | 1 |
| 15 | Target-responsive structural switching for nucleic acid-based sensors 显示文摘 | Li Di Song Shiping Fan Chunhai | 2010 | Accounts of Chemi-cal Research2010，43，5： | 1 |
| 16 | Aptamer-based biosensors显示文摘 | Shiping Song Lihua Wang Jiang Li Chunhai Fan Jianlong Zhao | 2007 | Trends in Analytical Chemistry2007，，2： | 1 |
| 17 | An On - Nanoparticle Roiling - Circle Amplification Platform for Uhrasensitive Protein Detection in:Bio- logical Fluids 显示文摘 | Juan Yan Shiping Song Bing Li Qingzhi Zhang Qing Huang Hua Zhang Chunhai Fan | 2010 | small2010，22，6： | 1 |
| 18 | Expression Profiles of 10,422 Genes at Early Stage of Low Nitrogen Stress in Rice Assayed using a cDNA Microarray显示文摘 | Xingming Lian Shiping Wang Jianwei Zhang Qi Feng Lida Zhang Danlin Fan Xianghua Li Dejun Yuan Bin Han Qifa Zhang | 2006 | Plant Molecular Biology2006，，5： | 1 |
| 19 | Evaluation of the use of high CO 2 concentrations and cold storage to control of Monilinia fructicola on sweet cherries显示文摘 | Shiping Tian Qing Fan Yong Xu Yi Wang Aili Jiang | 2001 | Postharvest Biology and Technology2001，，1： | 1 |
| 20 | Matroidal structure of rough sets and its characterization to attribute reduction显示文摘 | WANG Shiping ZHU Qingxin ZHU William MIN Fan | 2012 | KnowledgeBased Systems2012，36，： | 1 |
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