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| 1 | Clinical and biochemical indexes from 2019-nCoV infected patients linked to viral loads and lung injury显示文摘The outbreak of the 2019-nCoV infection began in December 2019 in Wuhan,Hubei province,and rapidly spread to many provinces in China as well as other countries.Here we report the epidemiological,clinical,laboratory,and radiological characteristics,as well as potential biomarkers for predicting disease severity in 2019-nCoV-infected patients in Shenzhen,China.All 12 cases of the 2019-nCoV-infected patients developed pneumonia and half of them developed acute respiratory distress syndrome (ARDS).The most common laboratory abnormalities were hypoalbuminemia,lymphopenia,decreased percentage of lymphocytes (LYM) and neutrophils (NEU),elevated C-reactive protein (CRP) and lactate dehydrogenase (LDH),and decreased CD8 count.The viral load of 2019-nCoV detected from patient respiratory tracts was positively linked to lung disease severity.ALB,LYM,LYM (%),LDH,NEU (%),and CRP were highly correlated to the acute lung injury.Age,viral load,lung injury score,and blood biochemistry indexes,albumin (ALB),CRP,LDH,LYM (%),LYM,and NEU (%),may be predictors of disease severity.Moreover,the Angiotensin Ⅱlevel in the plasma sample from 2019-nCoV infected patients was markedly elevated and linearly associated to viral load and lung injury.Our results suggest a number of potential diagnosis biomarkers and angiotensin receptor blocker (ARB) drugs for potential repurposing treatment of 2019-nCoV infection. | Yingxia Liu Yang Yang Cong Zhang Fengming Huang Fuxiang Wang Jing Yuan Zhaoqin Wang Jinxiu Li Jianming Li Cheng Feng Zheng Zhang Lifei Wang Ling Peng Li Chen Yuhao Qin Dandan Zhao Shuguang Tan Lu Yin Jun Xu Congzhao Zhou Chengyu Jiang Lei Liu | 2020 | Science China(Life Sciences)2020，63，3： | 157 |
| 2 | Elevated plasma levels of selective cytokines in COVID-19 patients reflect viral load and lung injury显示文摘A recent outbreak of pneumonia in Wuhan,China was found to be caused by a 2019 novel coronavirus(2019-nCoV or SARS-CoV-2 or HCoV-19).We previously reported the clinical features of 12 patients with2019-n Co V infections in Shenzhen,China.To further understand the pathogenesis of COVID-19 and find better ways to monitor and treat the disease caused by 2019-n Co V,we measured the levels of 48 cytokines in the blood plasma of those 12 COVID-19 patients.Thirty-eight out of the 48 measured cytokines in the plasma of 2019-n Co V-infected patients were significantly elevated compared to healthy individuals.Seventeen cytokines were linked to 2019-nCoV loads.Fifteen cytokines,namely M-CSF,IL-10,IFN-α2,IL-17,IL-4,IP-10,IL-7,IL-1 ra,G-CSF,IL-12,IFN-γ,IL-1α,IL-2,HGF and PDGF-BB,were strongly associated with the lung-injury Murray score and could be used to predict the disease severity of2019-n Co V infections by calculating the area under the curve of the receiver-operating characteristics.Our results suggest that 2019-nCoV infections trigger extensive changes in a wide array of cytokines,some of which could be potential biomarkers of disease severity of 2019-n Co V infections.These findings will likely improve our understanding of the immunopathologic mechanisms of this emerging disease.Our results also suggest that modulators of cytokine responses may play a therapeutic role in combating the disease once the functions of these elevated cytokines have been characterized. | Yingxia Liu Cong Zhang Fengming Huang Yang Yang Fuxiang Wang Jing Yuan Zheng Zhang Yuhao Qin Xiaoyun Li Dandan Zhao Shunwang Li Shuguang Tan Zhaoqin Wang Jinxiu Li Chenguang Shen Jianming Li Ling Peng Weibo Wu Mengli Cao Li Xing Zhixiang Xu Li Chen Congzhao Zhou William J Liu Lei Liu Chengyu Jiang | 2020 | National Science Review2020，7，6： | 6 |
| 3 | 亚毒性浓度顺铂联合紫花牡荆素体外诱导人卵巢癌细胞调亡(英文)显示文摘Objective: The aim of the study was to investigate the effect of Casticin (CAS) combination with Cisplatin (DDP) in sub-toxic concentration on apoptosis of human ovarian cancer HO-8910 cells in vitro and unravel the associated mechanisms. Methods: Human ovarian cancer HO-8910 cells were cultured in vitro. The inhibitory effect of CAS combination with DDP in sub-toxic concentration on viability of human ovarian cancer HO-8910 cells was evaluated by the MTT assay. Morphological changes of cell apoptosis were detected by Hoechst 33258 staining assay. Cell apoptosis rate was analyzed by flow cytometry. The protein expression level was analyzed by Western blot. Results: CAS in sub-toxic concentration and DDP in sub-toxic concentration could slightly inhibit Human ovarian cancer HO-8910 cells, but CAS combination with DDP in sub-toxic concentration significantly inhibited the growth of HO-8910 cells, and growth inhibition rate was increased drastically compared with the control group (P﹤0.01), and the inhibiting effect showed synergistic action. Human ovarian cancer HO-8910 cells showed the typical morphological changes of apoptosis and apoptosis rate markedly increased when they were exposed to CAS combination with DDP in sub-toxic concentration for 48 h. Western blot showed that the expression of bcl-2 protein was down-regulated and protein level of caspase-3 was activated by CAS combination with DDP in sub-toxic concentration. Conclusion: CAS combination with DDP in sub-toxic concentration could inhibit the cells growth and lead to cell apoptosis in human ovarian cancer HO-8910 cells. And the down-regulation of bcl-2 protein expression and activation of caspase-3 protein might contribute to CAS combination with DDP in sub-toxic concentration in human cancer HO-8910 cells. | Jun Bai Guihuang Tan Li Chen Yingxia Ning | 2013 | The Chinese-German Journal of Clinical Oncology2013，12，1： | 3 |
| 4 | Rudimentary study on humanization of porcine red blood cells: Enzymatic removal of galactose-α1,3- galactose antigen from porcine red blood cell显示文摘The serological and biochemical characteriza-tion of porcine red blood cells (pRBCs) are similar to human red blood cells. Porcine erythrocytes are considered as an alternative source for human blood transfusion. But there exist galactose-?,3-galactose antigens (Gal?,3Gal?, 4GalNAcR, abbreviated 酖al antigen) on pRBCs, which can induce anti-aGal antibodies in human serum. The aGal epitopes are the major antigen responsible for hyperacute rejection in xenotransfusion. In this study, recombined soy-bean -galactosidase (rS?GalE) was used to remove the aGal antigens from pPRCs for humanization. The results showed that aGal antigen was cleared by rS?GalE and the structure and function of rS?GalE treated pRBC were normal. | WANG Jiexi GAO Xin BAI Yan REN Huiming TAN Yingxia ZHANG Yangpei | 2003 | Chinese Science Bulletin2003，48，4： | 2 |
| 5 | Influence of PEG additive and precursor concentration on the preparation of LaCoO3 film with perovskite structure显示文摘 | Yingxia Zhang Yongfa Zhu Ruiqin Tan | 2001 | Thin Solid Films2001，388，： | 1 |
| 6 | Brief report: a new profile of terminal N-acetyllaetosamines glycans on pig red blood cells and different expression of ct-galactose on Sika deer red blood cells and nucleated cells显示文摘 | Tan Yingxia Gong Feng Li Subo | 2010 | Glycoconjugate J2010，27，4： | 1 |
| 7 | Application of cL-N-acetylgalactosaminidase and ct-galactosidase in AB toO red blood ceils conversion显示文摘 | Gao Hongwei Li Subo Tan Yingxia | 2013 | Artif Ceils Nanomed Biotech- nol2013，41，1： | 1 |
| 8 | Down-regulation of αGal epitopes by co-transfection of α1,3-galactosidase gene and α1,2-fucosyltransferase gene显示文摘The polycarbohydrate structure of Galα1- 3Galβ1-4GluNAc-R (known as αGal epitopes of xenoantigen), produced by α1-3-galactosyltransferase (α1,3-GT) in the course of animal development, is the major xenoantigen on the cell surface of porcine which causes hyperacute rejection in pig-to-human xenotransplantation. Alpha-1,3-galactosi- dase (AGL), a hydrolytic enzyme, can remove the terminal α-1,3-galactosyl from the Galα1-3Galβ1-4GluNAc-R struc-ture resulting in cleaning αGal epitopes from the porcine cells. Alpha-1,2-fucosyltransferase (HT) can modify the sur-face carbohydrate phenotype of porcine cells, bringing about reduction of αGal epitopes expression. In this study, human AGL and HT gene were co-transfected to porcine fetal fibro-blast (PFFb) in equimolar concentration to reduce the xeno-antigen. Gene and protein of hAGL and HT were both de-tected to express at high level by RT-PCR and Western blot, respectively. There was an 84% reduction in αGal xenoanti-gen and an 82% increase in H antigen as assayed by flow cytometry in the AGL and HT gene co-transfected PFFb. The number and morphology of transgenic PFFb chromosome were normal. Findings indicate that Galα1-3Gal epitopes of PFFb could be down regulated by AGL and HT co-transfec- tion without deleterious effects on the chromosomal profile of the transgenic cell. | GONG Feng ZHANG Yangpei JIA Yanjun WANG Yingli TAN Yingxia TIAN shuguang | 2005 | Chinese Science Bulletin2005，50，23： | 0 |
| 9 | Avian influenza viruses suppress innate immunity by inducingtrans-transcriptional readthrough via SSU72显示文摘Innate immunity plays critical antiviral roles. The highly virulent avian influenza viruses (AIVs) H5N1, H7N9, and H5N6 can betterescape host innate immune responses than the less virulent seasonal H1N1 virus. Here, we report a mechanism by whichtranscriptional readthrough (TRT)-mediated suppression of innate immunity occurs post AIV infection. By using cell lines, mouselungs, and patient PBMCs, we showed that genes on the complementary strand (“trans” genes) influenced by TRT were involved inthe disruption of host antiviral responses during AIV infection. The trans-TRT enhanced viral lethality, and TRT abolishmentincreased cell viability and STAT1/2 expression. The viral NS1 protein directly bound to SSU72, and degradation of SSU72 inducedTRT. SSU72 overexpression reduced TRT and alleviated mouse lung injury. Our results suggest that AIVs infection induce TRT byreducing SSU72 expression, thereby impairing host immune responses, a molecular mechanism acting through the NS1-SSU72-trans-TRT-STAT1/2 axis. Thus, restoration of SSU72 expression might be a potential strategy for preventing AIV pandemics. | Yan Zhao Fengming Huang Zhen Zou Yuhai Bi Yang Yang Cong Zhang Qiang Liu Daozhen Shang Yiwu Yan Xiangwu Ju Song Mei Peng Xie Xiao Li Mingyao Tian Shuguang Tan Huijun Lu Zongsheng Han Kangtai Liu Yuqing Zhang Junbo Liang Zhu Liang Qingchao Zhang Jiahui Chang William JLiu Cong Feng Tanshi Li Michael Q.Zhang Xiaoyue Wang George FGao Yingxia Liu Ningyi Jin Chengyu Jiang | 2022 | Cellular & Molecular Immunology2022，19，6： | 0 |
| 10 | Four temporin-derived peptides exhibit antimicrobial and antibiofilm activities against methicillin-resistant Staphylococcus aureus显示文摘Temporin-GHa(GHa)was cloned from Hylarana guentheri,showing a weak antimicrobial activity.In order to improve its bactericidal efficacy,GHaR6R,GHaR7R,GHaR8R and GHaR9W were designed and synthesized.Compared to the parent peptide,the GHa-derived peptides show potent antimicrobial activities against methicillin-resistant Staphylococcus aureus(MRSA),which is the main pathogen with high morbidity and mortality that causes various infections in humans.These peptides exert bactericidal actions on MRSA by permeabilizing the cytoplasmic membranes and damaging membrane integrity.All of the four peptides exhibit excellent stability under harsh conditions,including extreme temperature and salts.Furthermore,they inhibit the formation of biofilm and eradicate mature biofilm of MRSA.The GHa-derived peptides decrease bacterial surface hydrophobicity,autoaggregation and polysaccharide intercellular adhesion synthesis in concentration-dependent manner.Real-time quantitative reverse transcription PCR analysis revealed that the peptides downregulatethe expression of adhesion genes icaADBC involved in biofilm formation.Except for GHaR7R,the other three peptides have low hemolytic toxicity against human erythrocytes.In the presence of human erythrocytes,GHaR7R,GHaR8R and GHaR9W interact with MRSA preferentially.GHaR6R,GHaR8R and GHaR9W show less toxicity toward normal cells HL-7702 and hFOB1.19.These results suggest that the GHa-derived peptides may be promising antimicrobial candidates against MRSA infections. | Xiuchuan Tan Shenghong Xie Xiao Jin Ruiying Zhu Shuangshuang Wei Yanting Song Xi Xie Rong Wang Lushuang Li Manchuriga Wang Yingxia Zhang | 2022 | Acta Biochimica et Biophysica Sinica2022，54，3： | 0 |
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