|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Tribenuron-Methyl Induces Male Sterility through Anther-Specific Inhibition of Acetolactate Synthase Leading to Autophagic Cell Death显示文摘Tribenuron 甲基(TM ) 是禁止分支链的强大的 sulfonylurea 除草剂由指向 acetolactate synthase (ALS ) 的催化子单元(CSR1 ) 的氨基酸(BCAA ) 生合成。由在低剂量的 TM 的叶的喷洒的男绝育的选择正式就职广泛地在油菜籽(Brassica 马来麝) 为混合种子生产被使用了;然而,内在的机制仍然保持未知。这里,我们 reportgreater TM 累积和在花药的 BCAA 饥饿比在在 TM 申请以后的叶子和茎。csr1-1D (aCSR1 异种) 的组成或花药特定的表示消除了花药选择的 ALS 抑制并且在油菜籽和 Arabidopsis 颠倒了导致 TM 的男无菌的显型。TM 涂抹茎实验的结果,在叶肉或韧皮部由 TM 新陈代谢基因 Bel 的指向的表示在导致 TM 的男绝育的花药和回复与很少 TM 累积的观察结合了,建议叶水花的 TM 通过叶肉和韧皮部主要对花药极运输。显微镜学和 immunoblotting 揭示了那 autophagy,在房间死亡期间导致的一个体积降级过程,在导致 TM 的男无菌的花药并且由被提高花药特定 ALS 击倒。而且,导致 TM 的花粉流产被 autophagy 禁止者 3 麻省显著地禁止。这些数据最终建议 TM 对花药极运输,导致经由花药特定的 ALS 抑制的 BCAA 饥饿并且,,在花药的 autophagic 房间死亡。 | Lun Zhao Xue Jing Li Chen Yingjun Liu Yanan Su Tingting Liu Changbin Gao Bin Yi Jing Wen Chaozhi Ma Jinxing Tu Jitao Zou Tingdong Fu Jinxiong Shen | 2015 | Molecular Plant2015，8，12： | 7 |
| 2 | Schisandrol A protects AGEs-induced neuronal cells death by allosterically targeting ATP6V0d1 subunit of V-ATPase显示文摘Diabetes have been shown to cause progressive neuronal injury with pain and numbness via advanced glycation end-products(AGEs)-induced neuronal cell apoptosis;however, the valuable drug targets for diabetic neuropathy have been poorly reported so far. In this study, we discovered a natural small-molecule schisandrol A(SolA) with significant protective effect against AGEs-induced neuronal cell apoptosis. ATP6V0D1, a major subunit of vacuolar-type ATPase(V-ATPase) in lysosome was identified as a crucial cellular target of SolA. Moreover, SolA allosterically mediated ATP6V0D1 conformation via targeting a unique cysteine 335 residue to activate V-ATPase-dependent lysosomal acidification.Interestingly, SolA-induced lysosome pH downregulation resulted in a mitochondrial-lysosomal crosstalk by selectively promoting mitochondrial BH3-only protein BIM degradation, thereby preserving mitochondrial homeostasis and neuronal cells survival. Collectively, our findings reveal ATP6V0D1 is a valuable pharmacological target for diabetes-associated neuronal injury via controlling lysosomal acidification, and also provide the first small-molecule template allosterically activating V-ATPase for preventing diabetic neuropathy. | Xiaoqing Zhou Shaoyang Zhao Tingting Liu Lu Yao Meimei Zhao Xiaoming Ye Xiaowen Zhang Qiang Guo Pengfei Tu Kewu Zeng | 2022 | Acta Pharmaceutica Sinica B2022，12，10： | 3 |
| 3 | Polymerase mutations rtN238R,rtT240Y and rtN248H of hepatitis B virus decrease susceptibility to adefovir显示文摘Long term antiviral therapy with nucleos(t)ide analogs(NAs) may lead to the emergence of drug-resistance viral mutants in chronic hepatitis B virus(HBV) patient.The purpose of this study was to identify adefovir dipivoxil(ADV) resistance mutations of HBV polymerase and determine effective drugs to replace ADV.The reverse transcriptase(RT) coding region was PCR-amplified using HBV DNA extracted from patient blood samples and sequenced.Nineteen substitution mutations were detected.Among them,rtN238R,rtT240Y and rtN248H were often observed in patients receiving ADV administration.These three potential drug resistant sites were introduced into HBV replication-competent plasmids.The in vitro susceptibility of both wild-type(WT) and mutant-type(MT) HBV to NAs was analyzed by Southern blotting and quantitative real-time PCR.The rtN238R,rtT240Y and rtN248H substitutions had no obvious effect on HBV DNA replication or gene expression.The in vitro susceptibility analysis showed that rtN238R,rtT240Y and rtN248H substitutions were responsible for the reduced susceptibility to ADV,and demonstrated a 5.42-,2.89-and 5.72-fold increase in resistance towards ADV,respectively.However,HBV harbored these mutations retained normal susceptibility to LMV,LdT,ETV and TDF. | QIN Bo PEI RongJuan HE TingTing HUANG ZhaoHui PAN GuoShao TU ChunYu LU MengJi CHEN XinWen | 2013 | Chinese Science Bulletin2013，58，15： | 1 |
| 4 | Treadmill exercise promotes angiogenesis in the ischemic penumbra of rat brains through caveolin-1/VEGF signaling pathways显示文摘 | Yong Gao Yun Zhao Jingzi Pan Liu Yang Tingting Huang Xiaomin Feng Chao Li Shujing Liang Dingfu Zhou Chan Liu Fengxia Tu Cai Tao Xiang Chen | 2014 | Brain Research2014，，： | 1 |
| 5 | Bn.YCO affects chloroplast development in Brassica napus L.显示文摘Mature chloroplasts,as the main sites of photosynthesis,are essential for seedling growth in higher plants.Loss of function of genes involved in chloroplast development changes plant phenotype.We obtained a YELLOW COTYLEDON (YCO) mutant in rapeseed (Brassica napus L.) using CRISPR-Cas9.Bn.YCO,a gene of unknown function,has two homologous copies (Bna A01.YCO and Bna C01.YCO) in B.napus.Homozygous mutation of these two homologs resulted in yellow cotyledons and chlorotic true leaves.Transmission electron microscopy revealed that the formation of thylakoid membranes was inhibited in yellow cotyledons.Sequence similarity search revealed that YCO was conserved in different species,and a subcellular location assay verified that Bn.YCO was located in the chloroplast.Bn.YCO was expressed in multiple tissues,most highly in cotyledons.Knockout of Bn.YCO blocked the transcription of plastid genes,especially those of photosystem genes transcribed by plastid-encoded polymerase.Transcriptome sequencing showed that the majority of genes involved in ribosome assembly and photosynthesis were down-regulated in Bn.yco mutants.These results suggested that loss of function of Bn.YCO affected plastid gene transcription,which influenced chloroplast biogenesis in rapeseed seedlings. | Tingting Liu Baolong Tao Hanfei Wu Jing Wen Bin Yi Chaozhi Ma Jinxing Tu Tingdong Fu Lixia Zhu Jinxiong Shen | 2021 | The Crop Journal2021，9，5： | 1 |
| 6 | Rheological behavior of polyarylsulfone 1-butyl-3-methylimidazolim chloride solution 显示文摘 | Tu Xiaoping Zhang Yumei Zhao Tingting | 2006 | Journal of Macromolecular Science Part B-Physics2006，45，4： | 1 |
| 7 | Exploring metro vibrancy and its relationship with built environment:a cross-city comparison using multi-source urban data显示文摘Recent urban transformations have led to critical reflections on the blighted urban infrastruc-tures and called for re-stimulating vital urban places.Especially,the metro has been recognized as the backbone infrastructure for urban mobility and the associated economy agglomeration.To date,limited research has been devoted to investigating the relationship between metro vitality and built environment in mega-cities empirically.This paper presents a multisource urban data-driven approach to quantify the metro vibrancy and its association with the underlying built environment.Massive smart card data is processed to extract metro ridership,which denotes the vibrancy around the metro station in physical space.Social media check-ins are crawled to measure the vitality of metros in virtual spaces.Both physical and virtual vibrancy are integrated into a holistic metro vibrancy metric using an entropy-based weighting method.Certain built environment characteristics,including land use,transportation and buildings are modeled as independent variables.The significant influences of built environ-mental factors on the metro vibrancy are unraveled using the ordinary least square regression and the spatial lag model.With experiments conducted in Shenzhen,Singapore and London,this study comes up with a conclusion that spatial distributions of metro vibrancy metrics in three cities are spatially autocorrelated.The regression analysis suggests that in all the three cities,more affluent urban areas tend to have higher metro virbrancy,while the road density,land use and buildings tend to impact metro vibrancy in only one or two cities.These results demonstrate the relationship between the metro vibrancy and built environment is affected by complex urban contexts.These findings help us to understand metro vibrancy thus make proper policy to re-stimulate the important metro infrastructure in the future. | Wei Tu Tingting Zhu Chen Zhong Xiaohu Zhang Yang Xu Qingquan Li | 2022 | Geo-Spatial Information Science2022，25，2： | 0 |
| 8 | Genome-wide association study revealed genetic variations of ABA sensitivity controlled by multiple stress-related genes in rice显示文摘Abscisic acid(ABA)is a critical phytohormone that regulates multiple physiological processes including plant growth and stress tolerance.The core ABA signaling pathway has been well established,but genetic variations mediating ABA responses remain largely unknown.In this study,we performed genome-wide association study(GWAS)to identify loci and genes associated with ABA sensitivity(reflected by seed germination inhibition by ABA)in a panel of 425 rice accessions.The seed germination assay revealed that Aus and indica rice had stronger ABA sensitivity than japonica rice.A total of 48 non-redundant association loci were detected in the indica subpopulation and whole population,and 386 genes in these loci were responsive to ABA or abiotic stresses.Eight association loci were overlapped with previously reported loci for yield under drought stress or for droughtindicative image traits.Haplotype analyses of important candidate genes such as OsSAPK6,a key component in the ABA signaling core,were performed to identify key SNPs/InDels that may affect gene functions through promoter activity regulation,amino acid variation,or gene splicing.These results provide insights into the genetic basis of ABA sensitivity related to stress responses. | Lei Peng Tingting Xie Zilong Guo Xiaokai Li Yu Chang Haifu Tu Shengchang Wang Nai Wu Yilong Yao Lizhong Xiong | 2021 | Stress Biology2021，1，1： | 0 |
| 9 | A novel dense retrieval framework for long document retrieval显示文摘1 Introduction.Inspired by the impressive success of BERT[1]in various NLP applications,researchers have attempted to apply pretrained language models to information retrieval,and existing BERT-based retrieval models obtain improved performance on passage retrieval[2-4].Since BERT has the limitation that the maximum length of tokens is only 512,however,simply applying those models to the task of long document retrieval derives suboptimal results. | Jiajia WANG Weizhong ZHAO Xinhui TU Tingting HE | 2023 | Frontiers of Computer Science2023，17，4： | 0 |
| 10 | A polygalacturonase gene OsPG1 modulates water homeostasis in rice显示文摘A dynamic plant architecture is the basis of plant adaptation to changing environments.Although many genes regulating leaf rolling have been identified,genes directly associated with water homeostasis are largely unknown.Here,we isolated a rice mutant,dynamic leaf rolling 1(dlr1),characterized by‘leaf unfolding in the morning-leaf rolling at noon-leaf unfolding in the evening’during a sunny day.Water content was decreased in rolled leaves and water sprayed on leaves caused reopening,indicating that in vivo water deficiency induced the leaf rolling.Map-based cloning and expression tests demonstrated that an A1400G single base mutation in Oryza sativa Polygalacturonase 1(OsPG1)/PHOTO-SENSITIVE LEAF ROLLING 1(PSL1)was responsible for the dynamic leaf rolling phenotype in the dlr1 mutant.OsPG1 encodes a polygalacturonase,one of the main enzymes that degrade demethylesterified homogalacturonans in plant cell walls.OsPG1 was constitutively expressed in various tissues and was enriched in stomata.Mutants of the OsPG1 gene exhibited defects in stomatal closure and decreased stomatal density,leading to reduced transpiration and excessive water loss under specific conditions,but had normal root development.Further analysis revealed that mutation of OsPG1 led to reduced pectinase activity in the leaves and increased demethylesterified homogalacturonans in guard cells.Our findings reveal a mechanism by which OsPG1 modulates water homeostasis to control dynamic leaf rolling,providing insights for plants to adapt to environmental variation. | Qinwen Zou Ranran Tu Jiajun Wu Tingting Huang Zhihao Sun Zheyan Ruan Hongyu Cao Shihui Yang Xihong Shen Guanghua He Hong Wang | 2024 | The Crop Journal2024，12，1： | 0 |
| 11 | Low Concordance Between Blood Pressures Measured in Periodic Health Examinations and in a Workplace-Based Hypertension Management Program显示文摘Poor adherence to standard protocols of blood pressure(BP)measurement in routine clinical practice leads to higher readings than“research-quality”measurements.Whether this phenomenon exists in periodic health examinations was unknown.We aimed to explore the concordance between BP measurements in periodic health examinations and those measured following a standard measurement protocol.We used data from the Kailuan Study,an ongoing longitudinal cohort study in China,of which participants received biennial health examinations in health management centers.In addition,BPs were measured following standard protocols in a workplace-based hypertension management program nested in the Kailuan Study.We compared BP readings of the same person between the two settings using generalized linear mixed-effects models.A total of 3988 men(the mean age was 44.9 years)had at least two BP measurements both in health examinations and management program with a time interval between the two settings that less than 90 days.The mean systolic blood pressures(SBP)and diastolic blood pressures(DBP)in health examinations were 4.2(95%CI 3.9–4.5)mm Hg and 3.3(95%CI 3.1–3.5)mm Hg higher than those in the management program,respectively.Bland–Altman analyses showed the wide agreement inter-vals ranging from-27.7-to 36.5-mm Hg for SBP and-18.3-to 24.7-mm Hg for DBP.In conclusion,BP measurements in periodic health examinations were generally higher than BPs measured following a standard protocol.Our findings highlight the importance of standard BP measurement to avoid overestimation of hypertension prevalence and treatment initiation. | Jun-Xiang Chen Yan-Feng Zhou Tingting Geng Simiao Chen Shuohua Chen Guodong Wang Yan-Bo Zhang Yi Wang Zhou-Zheng Tu Gang Liu Shouling Wu An Pan | 2022 | Phenomics2022，2，6： | 0 |
| 12 | Tprn is essential for the integrity of stereociliary rootlet in cochlear hair cells in mice显示文摘Tprn encodes the taperin protein,which is concentrated in the tapered region of hair cell stereocilia in the inner ear.In humans,TPRN mutations cause autosomal recessive nonsyndromic deafness(DFNB79)by an unknown mechanism.To determine the role of Tprn in hearing,we generated Tprn-null mice by clustered regularly interspaced short palindromic repeat/Cas9 genome-editing technology from a CBA/CaJ background.We observed significant hearing loss and progressive degeneration of stereocilia in the outer hair cells of Tprn-null mice starting from postnatal day 30.Transmission electron microscopy images of stereociliary bundles in the mutant mice showed some stereociliary rootlets with curved shafts.The central cores of the stereociliary rootlets possessed hollow structures with surrounding loose peripheral dense rings.Radixin,a protein expressed at stereocilia tapering,was abnormally dispersed along the stereocilia shafts in Tprn-null mice.The expression levels of radixin andβ-actin significantly decreased.We propose that Tprn is critical to the retention of the integrity of the stereociliary rootlet.Loss of Tprn in Tprn-null mice caused the disruption of the stereociliary rootlet,which resulted in damage to stereociliary bundles and hearing impairments.The generated Tprn-null mice are ideal models of human hereditary deafness DFNB79. | Yuqin Men Xiujuan Li Hailong Tu Aizhen Zhang Xiaolong Fu Zhishuo Wang Yecheng Jin Congzhe Hou Tingting Zhang Sen Zhang Yichen Zhou Boqin Li Jianfeng Li Xiaoyang Sun Haibo Wang Jiangang Gao | 2019 | Frontiers of Medicine2019，13，6： | 0 |
| 13 | Biomimetic construction of oriented lamellar Col/nHAP composite scaffolds and mediation of macrophages to promote angiogenesis and bone regeneration显示文摘Pore characteristics have been identified as key design parameters for osteoimmunomodulation.The strategy reported here is to create an appropriate immune microenvironment by regulating pore characteristics of scaffolds,thereby promoting early angiogenesis and enhancing osteogenesis.A series of collagen/nanohydroxyapatite(Col/nHAP)composite scaffolds with ordered lamellar structures and different layer spacings were prepared by mimicking the ordered lamellar topology of the bone matrix.Our research indicated that the layer spacing and ordered topology of the scaffold exerted an important influence on phenotype transformation of macrophages and the secretion of angiogenic factors.The Col/nHAP-O(135)with large layer spacing not only supported cell attachment and diffusion in vitro,but also promoted early angiogenesis by timely switching from M1 to M2 macrophage phenotype.In vivo data showed that the layer spacing and the ordered structure of the scaffold synergistically regulated the inflammatory response and triggered macrophages to secrete more angiogenesis related cytokines.Col/nHAP-O(135)considerably promoted the neovascularization and new bone formation in the defect site,indicating that Col/nHAPO(135)could significantly enhance the osteogenic activity of stem cells with the involvement of macrophages. | Tingting Huang Tao Huang Pin Luo Di Xiao Yiping Huang Shenyu Yang Rong Zeng Mei Tu | 2023 | Frontiers of Materials Science2023，17，4： | 0 |
| 14 | Functional Xeno Nucleic Acids for Biomedical Application显示文摘Functional nucleic acids(FNAs)refer to a type of oligonucleotides with functions over the traditional genetic roles of nucleic acids,which have been widely applied in screening,sensing and imaging fields.However,the potential application of FNAs in biomedical field is still restricted by the unsatisfactory stability,biocompatibility,biodistribution and immunity of natural nucleic acids(DNA/RNA).Xeno nucleic acids(XNAs)are a kind of nucleic acid analogues with chemically modified sugar groups that possess improved biological properties,including improved biological stability,increased binding affinity,reduced immune responses,and enhanced cell penetration or tissue specificity.In the last two decades,scientists have made great progress in the research of functional xeno nucleic acids,which makes it an emerging attractive biomedical application material.In this review,we summarized the design of functional xeno nucleic acids and their applications in the biomedical field. | TU Tingting HUAN Shuangyan KE Guoliang ZHANG Xiaobing | 2022 | Chemical Research in Chinese Universities2022，38，4： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员