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| 1 | RNA Interference-Mediated Gene Silencing of Vascular Endothelial Growth Factor显示文摘OBJECTIVE To inhibit the expression of the vascular endothelial growth factor (VEGF) by RNA interference, and to observe the effect in different cells line. METHODS Using the services of E-RNAi, we designed and constructed two kinds of shRNAs expression vectors which were aimed at the VEGF gene. These vectors were then transfected into HEK293, colon cancer HT29, Hela and HepG2 cells by LipofectamineTM 2000. The level of VEGF mRNA was determined by RT-PCR and Northern blotting and the VEGF expression was examined by immunofluoresence staining. RESULTS The two kinds of VEGF specific shRNAs expression vectors were found to efficiently inhibit the expression of VEGF in HEK293 and HT29 cells by RT-PCR analysis, with inhibition rates of 72% and 42%, respectively; but the inhibition rates were reduced to 28% in Hela cells and 13% in HepG2 cells. Northern blotting showed that the inhibition rates of VEGF mRNA expression were 88% and 89% in HEK293 and HT29 cells, respectively. The inhibition rate of VEGF protein expression in HT29 cells was 73% based on immunofluoresence staining. CONCLUSION The expression of VEGF was inhibited by RNA interference, but differed with various cells lines, showing that RNA interference was cell-line dependent. | Tiejun Li Jianning Song Kai Kang Zanlan Hu Tongchuan He Bingqiang Zhang Caiquan Zhang | 2006 | Chinese Journal of Clinical Oncology2006，3，6： | 1 |
| 2 | Association of Interleukin 6 gene polymorphisms with genetic susceptibilities to spastic tetraplegia in males: A case-control study显示文摘 | Mingjie Chen Tongchuan Li Sheyu Lin Dan Bi Dengna Zhu Qing Shang Caiyun Ma Honglian Wang Lei Wang Yiting Zhang Lin He Changlian Zhu Qinghe Xing | 2013 | Cytokine2013，，： | 1 |
| 3 | Development of sensor system for real-time measurement of droplet deposition of agricultural sprayers显示文摘During the chemical application process,droplet deposition on a target is an important reference indicator for evaluating the spraying technique and its performance.In order to quickly obtain deposition results in the field,this study proposed a novel system based on surface humidity sensors.The basic principle is to convert the measured physical quantity change into a capacitance change,thereby realizing the physical quantity to electrical signal conversion.An Android application for mobile terminal and the corresponding coordinator were developed,which allowed operators to control multiple sensors simultaneously through the Bluetooth.The soluble tracer detected by spectrophotometer was used to calibrate the system.The obtained results indicated a good correlation between deposition volume and voltage increment output from the newly developed system(R2 of the six nozzles with Dv0.5 ranging from 107.28μm to 396.20μm were 0.8674-0.9729),and a power regression model based on the least squares technique(R2=0.8022)was developed.In the field test,the system exhibited an optimal performance in predicting the deposition volume.Compared with the conventional method of measuring tracer concentration,the deviation was less than 10%.In addition,the system exhibited good fitting curve of the deposition distribution with droplet number results measured by the water sensitive paper method. | Longlong Li Ruirui Zhang Liping Chen Tongchuan Yi Gang Xu Daxuan Xue Qing Tang Linhuan Zhang Andrew John Hewitt Yining An | 2021 | International Journal of Agricultural and Biological Engineering2021，14，5： | 1 |
| 4 | Method for UAV spraying pattern measurement with PLS model based spectrum analysis显示文摘Unmanned aerial vehicle(UAV)chemical application is widely used for crop protection,and spraying pattern is one of the most important factors that influence the chemical control efficacy.A method for UAV spraying pattern measurement with partial least squares(PLS)model based spectrum analysis was proposed in this study to measure the UAV spraying pattern more accurately.The method involved the steps of fluorescent tracer solution spray and its droplets collection,the spectrum on paper strip acquiring,spectrum processing and analysis,PLS modeling.In order to verify the applicability of the method and obtain the parameters of the PLS model,UAV spraying experiments were performed in the field.Then Fluorescent tracer solution was sprayed and its droplets are collected by paper strip,and the original spectrum on paper strip obtained by a spectrometer was processed by the Savitzky-Golay and standard normalized variable(SNV)method.The prediction model of coverage rate selected as the droplet deposition parameter to measure the UAV spraying pattern,was established by using PLS method.To verify the superiority of the PLS model,a traditional linear regression(LR)model of coverage rate was established simultaneously.The results demonstrate that the method with PLS model based spectrum analysis can measure the UAV spraying pattern effectively,and PLS model has a better performance of RV2=0.94 and RMSEP=0.9446 than that of the LR model. | Ruirui Zhang Yao Wen Longlong Li Liping Chen Gang Xu Yanbo Huang Qing Tang Tongchuan Yi | 2020 | International Journal of Agricultural and Biological Engineering2020，13，3： | 0 |
| 5 | Spray drift characteristics of pulse-width modulation sprays in wind tunnel显示文摘Pulse-width modulation(PWM)sprays can improve flow accuracy by adjusting duty cycle and frequency signal which accurately controls the relative proportion of opening time of solenoid valve.The objective of this research was to determine the impacts of PWM duty cycle and frequency on spray drift characteristic.Spray tests were conducted in a wind tunnel with a PWM variable-rate spraying system.The airborne drift and sediment drift were determined with tracer method,and the drift potential reduction(DPR)compared with reference condition of 100%duty cycle at vertical profile and horizontal planes were calculated,respectively.The results show that,at a given frequency,droplet size decreases with the increase of duty cycle,the main reason is that the liquid does not reach full pattern development at lower duty cycle.Duty cycle has a greater impact than the frequency on spray drift,the influence weights of duty cycle on airborne drift and sediment drift were 88.32%and 77.89%,respectively.At a lower PWM frequency,in addition to the droplet size,the spray drift may be affected by the pulsed spray pattern.From the perspective of reducing spray drift,it is recommended that the PWM duty cycle should be set in the range of 20%-70%to reduce the potential drift in PWM sprays.This research provides a pesticide drift reduction scheme for variable spraying technology,which can serve as a theoretical basis for PWM parameter selection. | Longlong Li Liping Chen Ruirui Zhang Qing Tang Tongchuan Yi Boqin Liu Wei Deng | 2022 | International Journal of Agricultural and Biological Engineering2022，15，4： | 0 |
| 6 | Factors controlling deep-profile soil organic carbon and water storage following Robinia pseudoacacia afforestation of the Loess Plateau in China显示文摘Background:Afforestation is a common and effective approach used for the restoration of degraded ecosystems worldwide.In China,Robinia pseudoacacia(RP)is among the main non-native tree species and has been widely planted in revegetation of the Loess Plateau.However,owing to uncertainties regarding soil water consumption and carbon sequestration,it is necessary to assess the suitability and sustainability of R.pseudoacacia in restoration.In this study,we aimed to analyse the dynamic effects of R.pseudoacacia forest on soil carbon storage(SCS)and soil water storage(SWS).Specifically,we investigated the association between soil water content(SWC)and soil organic carbon(SOC)and underlying factors in the 0-500-cm profile of a 10-to 50-year-old chronosequence.Results:The results obtained indicated that the dynamics of SWS and SCS on this time scale could be divided into an initial reduction phase(the initial 20 years after afforestation)and subsequent recovery(20-50 years after afforestation).Compared with in the abandoned land(AL),the net accumulation of SCS in R.pseudoacacia forest was 2.51 Mg·ha^(-1)at 50 years after afforestation,whereas there was a 398.76-mm deficit in SWS.Additionally,the natural succession of R.pseudoacacia forest has contributed to the continuous change in stand structure(e.g.vegetation cover(VC),understory vegetation coverage(UVC),and litter biomass(LB)).Conclusions:These findings indicate that vegetation restoration increases carbon sequestration while causing soil water deficit.Furthermore,stand density(SD)was established to make a predominant contribution to the dynamics of SWS and SCS via its effects in altering vegetation,soil,and litter characteristics.Therefore,high-density plantation forests in the Loess Plateau area should be appropriately thinned to reduce the density of forest stands on the basis of soil erosion control and wind and sand fixation,so as to increase carbon sink with lower water consumption,thus realizing the synergistic development of soil carbon sequestration and water connotation. | Xi Yang Tongchuan Li Ming'an Shao | 2022 | Forest Ecosystems2022，9，6： | 0 |
| 7 | A simplified noncryogenic strategy to transport mesenchymal stem cells: Potential applications in cell therapy and regenerative medicine显示文摘With the rapid advances in stem cell research and po-tential cell-based therapies,there is an urgent need to develop safe and reliable cell transport strategies.Except for autologous stem cell-based therapies,allogeneic stem cell therapies and ex vivo genetically engineered cell therapies would require safe,efficient,and reliable cell preservation and transport methods. | Xiangyu Dong Yannian Gou Meichun Guo Jiamin Zhong Aohua Li Ailing Hao Wei Zeng Rex C.Haydon Hue H.Luu Russell R.Reid Tongchuan He Yan Xu Jiaming Fan | 2024 | Genes & Diseases2024，11，3： | 0 |
| 8 | Zokor activity promotes soil water infiltration in the Mu Us sandy land of northern Shaanxi,China显示文摘Zokors are common subterranean rodents that inhabit agricultural fields, shrublands, and grasslands in the arid and semi-arid regions of China. Zokor burrowing activities can alter soil structure and affect soil hydrological processes;however, there are few studies regarding their effects on soil preferential flow in the Mu Us sandy land. An evaluation of the effects of zokor disturbance on their habitat and soil water is important for understanding the ecological role of zokors in the soil ecosystem of the Mu Us sandy land. A field dye-tracing experiment was conducted in the Gechougou watershed on the southeastern edge of the Mu Us sandy land to investigate the effect of zokor burrowing activity on soil preferential flow characteristics. Our results showed that the density of zokor tunnels was the highest(0.40–0.46 m m^(-2)) under 30%–50% vegetation coverage and that the tunnels were approximately 3 cm from the surface.Both stained area ratio and stained path number were higher at sites with zokors than without zokors. Stained path widths were 10–80 and > 80 mm at zokor-harboring sites exhibiting homogeneous flow and heterogeneous finger flow, respectively. In the absence of zokors, homogeneous flow and highly interacted macropore flow were predominant. Soil water content below the zokor tunnels was higher than that above the tunnels. Moderate disturbance of soil structure by zokor activity facilitated soil water infiltration. These results enabled a better understanding of the effect of soil fauna on soil structure and hydrological processes and provided recommendations for ecological construction and renovation in arid and semi-arid regions. | Miao GAN Xuchao ZHU Xueqin YANG Xiaorong WEI Qingyin ZHANG Tongchuan LI Ming'an SHAO Meixia MI Xi YANG Mingyu CHEN | 2024 | Pedosphere2024，34，1： | 0 |
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