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| 1 | Rice CYP703A3, a cytochrome P450 hydroxylase, is essential for development of anther cuticle and pollen exine显示文摘Anther cuticle and pollen exine act as protective envelopes for the male gametophyte or pollen grain, but the mechanism underlying the synthesis of these lipidic polymers remains unclear. Previously, a tapetum‐expressed CYP703A3, a putative cytochrome P450 fatty acid hydroxylase, was shown to be essential for male fertility in rice(Oryza sativa L.). However,the biochemical and biological roles of CYP703A3 has not been characterized. Here, we observed that cyp703a3‐2 caused by one base insertion in CYP703A3 displays defective pollen exine and anther epicuticular layer, which differs from Arabidopsis cyp703a2 in which only defective pollen exine occurs. Consistently, chemical composition assay showed that levels of cutin monomers and wax components were dramatically reduced in cyp703a3‐2 anthers. Unlike the wide range of substrates of Arabidopsis CYP703A2, CYP703A3 functions as an in‐chain hydroxylase only for a specific substrate, lauric acid, preferably generating 7‐hydroxylated lauric acid. Moreover, chromatin immunoprecipitation and expression analyses revealed that the expression of CYP703A3 is directly regulated by Tapetum Degeneration Retardation, a known regulator of tapetum PCD and pollen exine formation. Collectively, our results suggest that CYP703A3 represents a conserved and diversified biochemical pathway for in‐chain hydroxylation of lauric acid required for the development of male organ in higher plants. | Xijia Yang Di Wu Jianxin Shi Yi He Franck Pinot Bernard Grausem Changsong Yin Lu Zhu Mingjiao Chen Zhijing Luo Wanqi Liang Dabing Zhang | 2014 | Journal of Integrative Plant Biology2014，56，10： | 21 |
| 2 | Interactions of OsMADS1 with Floral Homeotic Genes in Rice Flower Development显示文摘在繁殖开发期间,米饭植物开发决定最后的谷物收益的唯一的花器官。OsMADS1,象 SEPALLATA 一样疯盒子的基因之一,被解开了玩关键角色 inrice 花的机关身份说明和花的分裂组织确定性。然而,在调整花开发与另外的花的 homeotic 基因位于 OsMADS1 的相互作用下面的分子的机制 remainslargely 逃犯。在这个工作,我们在他们的蛋白质之中与物理相互作用一起与 B- , C- ,和 D 班基因学习了 OsMADS1 的基因相互作用。我们证明在 OsMADS1 和 OsMADS3 之间的物理、基因的相互作用为花的分裂组织活动维护和机关身份说明是必要的;当 OsMADS1 身体上并且遗传上在调整花的分裂组织确定性和 suppressingspikelet 分裂组织回复与 OsMADS58 交往时。我们提供了重要基因证据在花开发期间支持二米饭 C 班基因(OsMADS3 和 OsMADS58 ) 的 neofunctionalization。基因表示介绍和量的 RT-PCR 分析进一步表明 OsMADS1 影响涉及发信号的花的身份和荷尔蒙的许多基因的表达式,并且染色质 immunoprecipitation (芯片)-PCR 试金进一步证明 OsMADS17 是 OsMADS1 的直接目标基因。一起拿,这些结果表明 OsMADS1 在指定米饭有多样化的规章的功能花的机关和分裂组织身份,可能通过它和不同花的 homeotic 管理者的基因、物理的相互作用。 | Yun Hu Wanqi Liang Changsong Yin Xuelian Yang Baozhe Ping Anxue Li Ru Jia Mingjiao Chen Zhijing Luo Qiang Cai Xiangxiang Zhao Dabing Zhang Zheng Yuan | 2015 | Molecular Plant2015，8，9： | 10 |
| 3 | Microstructure, Microsegregation, and Mechanical Properties of Directional Solidified Mg–3.0Nd–1.5Gd Alloy显示文摘The microstructure, microsegregation, and mechanical properties of directional solidified Mg–3.0Nd–1.5Gd ternary alloys were experimentally studied. Experimental results showed that the solidification microstructure was composed of dendrite primary a(Mg) phase and interdendritic a(Mg) ? Mg12(Nd, Gd) eutectic and Mg5 Gd phase. The primary dendrite arm spacing k1 and secondary dendrite arm spacing k2 were found to be depended on the cooling rate R in the form k1= 8.0415 9 10-6R-0.279 and k2= 6.8883 9 10-6R-0.205, respectively, under the constant temperature gradient of40 K/mm and in the region of cooling rates from 0.4 to 4 K/s. The concentration profiles of Nd and Gd elements calculated by Scheil model were found to be deviated from the ones measured by EPMA to varying degrees, due to ignorance of the back diffusion of the solutes Nd and Gd within a(Mg) matrix. And microsegregation of Gd depended more on the growth rate, compared with Nd microsegregation. The directionally solidified experimental alloy exhibited higher strength than the non-directionally solidified alloy, and the tensile strength of the directionally solidified experimental alloy was improved,while the corresponding elongation decreased with the increase of growth rate. | Shaojun Liu Guangyu Yang Wanqi Jie | 2014 | Acta Metallurgica Sinica(English Letters)2014，27，6： | 6 |
| 4 | Microstructure Evolution during Solidification of AZ91D Magnesium Alloy in Semisolid显示文摘熄灭方法的液体被采用在半固体学习团结形态学和 AZ91D Mg 合金的微观结构。结果显示那冷却的率在团结结构之上有重要效果。在熄灭的液体的冷却的率下面,主要 alpha 阶段由在原来的高山上属于增长第一哈谷物,或独立成核和生长。高冷却率让主要一阶段在“破布”或树突形状成长。最容易溶解的团结以分裂生长和共生生长被执行。分裂生长在谷物边界形成不平、大的贝它阶段,当共生生长形成薄片状的结构的 eutectic 时。小液体在内以象的一样的方法基本上分享原来的 a-phasesolidifies 内部小粒的液体,但是由于液相的更少的数量,最容易溶解的团结主要在分裂模式被执行。 | Wuxiao WANG Bailing JIANG Sen YUAN Wanqi JIE Guangyu YANG | 2006 | Journal of Materials Science & Technology2006，22，3： | 5 |
| 5 | Regulatory network and genetic interactions established by OsMADS34 in rice inflorescence and spikelet morphogenesis显示文摘Grasses display highly diversified inflorescence architectures that differ in the arrangement of spikelets and flowers and determine cereal yields. However, the molecular basis underlying grass inflorescence morphogenesis remains largely unknown. Here we investigate the role of a functionally diversified SEPALLATA MADS-box transcription factor, OsMADS34, in regulating rice(Oryza sativa L.)inflorescence and spikelet development. Microarray analysis showed that, at the very early stages of inflorescence formation, dysfunction of OsMADS34 caused altered expression of 379 genes that are associated with protein modification and degradation, transcriptional regulation,signaling and metabolism activity. Genetic analysis revealed that OsMADS34 controls different aspects of inflorescence structure, branching and meristem activity synergistically with LAX PANICLE1(LAX1) and FLORAL ORGAN NUMBER4(FON4), as evidenced by the enhanced phenotypes of osmads34 lax1 and osmads34 fon4 compared with the single mutants. Additionally, double mutant between osmads34 and the sterile lemma defective mutant elongated empty glume(ele) displayed an enhanced phenotype, that is,longer and wider sterile lemmas that were converted into lemma/palea-like organs, suggesting that ELE and OsMADS34 synergistically control the sterile lemma development. OsMADS34 may act together with OsMADS15 in controlling sterile lemma development. Collectively, these findings provide insights into the regulatory function of OsMADS34 in rice inflorescence and spikelet development. | Qingcai Meng Xiaofeng Li Wanwan Zhu Li Yang Wanqi Liang Ludovico Dreni Dabing Zhang | 2017 | Journal of Integrative Plant Biology2017，59，9： | 5 |
| 6 | An Improved Hybrid Collaborative Filtering Algorithm Based on Tags and Time Factor显示文摘The Collaborative Filtering(CF) recommendation algorithm, one of the most popular algorithms in Recommendation Systems(RS), mainly includes memory-based and model-based methods. When performing rating prediction using a memory-based method, the approach used to measure the similarity between users or items can significantly influence the recommendation performance. Traditional CFs suffer from data sparsity when making recommendations based on a rating matrix, and cannot effectively capture changes in user interest. In this paper, we propose an improved hybrid collaborative filtering algorithm based on tags and a time factor(TTHybridCF), which fully utilizes tag information that characterizes users and items. This algorithm utilizes both tag and rating information to calculate the similarity between users or items. In addition, we introduce a time weighting factor to measure user interest, which changes over time. Our experimental results show that our method alleviates the sparsity problem and demonstrates promising prediction accuracy. | Chunxia Zhang Ming Yang Jing Lv Wanqi Yang | 2018 | Big Data Mining and Analytics2018，1，2： | 4 |
| 7 | Solidification Structure Formation of Undercooled Single Phase Alloys显示文摘Solidification structure variation of single phase alloy with undercooling prior to nucleation has been widely studied.The progress, especially during the last decade, is reviewed so as to give a comprehensive knowledge for it, in which the emphases are laid on the structure evolution mechanism and the potential application. Lastly, the future interesting subjects are presented. | Jinfu LI Yaohe ZHOU Wanqi JIE Gencang YANG | 2002 | Journal of Materials Science & Technology2002，18，4： | 3 |
| 8 | Immunogenicity of recombinant F4 (K88) fimbrial adhesin FaeG expressed in tobacco chloroplast显示文摘到通常对腹泻在植物生产新奇疫苗的可能性在新生、最新断奶的小猪发现了的测试, faeG 基因,编码主要 F4ac fimbrial 子单元蛋白质,被介绍进烟草叶绿体染色体。在在 spectinomycin 下面的二轮选择以后,我们将近获得了转基因的植物 homoplasmic。RNA 胶化污点分析显示了那 faeG 和抗菌素选择基因 aminoglycoside 3 adenylyltransferase (aadA ) 高度作为 dicistron 被抄录,当在 transplastomic 烟草的 recombinant FaeG 的翻译水平是大约 0.15% 全部的可溶的蛋白质时。在烟草叶绿体生产的 recombinant FaeG 的 immunogenicity 被 FaeG 特定的抗体在与导出叶绿体的 recombinant FaeG 刺激的老鼠 sera 能抵销的转基因的植物,以及结果的全部的可溶的蛋白质使免疫的老鼠被得到的观察证实在 vivo 的 F4ac enterotoxigenic Escherichia coli (ETEC ) 。这研究为用叶绿体表达式系统生产 ETEC 疫苗提供一种新选择。 | Huifeng Shen Bingjun Qian Weiwei Chen Zhenhua Liu Litao Yang Dabing Zhang Wanqi Liang | 2010 | Acta Biochimica et Biophysica Sinica2010，42，8： | 3 |
| 9 | Microstructure evolution,mechanical properties and diffusion behaviour of Mg-6Zn-2Gd-0.5Zr alloy during homogenization显示文摘The microstructure evolution and mechanical properties of Mg-6 Zn-2 Gd-0.5 Zr alloy during homogenization treatment were investigated. The as-cast alloy was found to be composed of dendritic primaryα-Mg matrix, α-Mg +W(Mg_3Zn_3Gd_2) eutectic along grain boundaries, and icosahedral quasicrystalline I(Mg_3Zn_6Gd) phase within α-Mg matrix. During homogenization process, α-Mg +W(Mg_3Zn_3Gd_2) eutectic and I phase gradually dissolved into a-Mg matrix, while some rod-like rare earth hydrides(GdH2)formed within α-Mg matrix. Both the tensile yield strength and the elongation showed a similar tendency as a function of homogenization temperature and holding time. The optimized homogenization parameter was determined to be 505℃ for 16 h according to the microstructure evolution. Furthermore,the diffusion kinetics equation of the solute elements derived from the Gauss model was established to predict the segregation ratio of Gd element as a function of holding time, which was proved to be effective to evaluate the homogenization effect of the experimental alloy. | Lei Xiao Guangyu Yang Yang Liu Shifeng Luo Wanqi Jie | 2018 | Journal of Materials Science & Technology2018，34，12： | 3 |
| 10 | Cellulose membranes as moisture-driven actuators with predetermined deformations and high load uptake显示文摘We report the synthesis of cellulose membranes from balsa wood with an exceptionally high responsivity to humidity change by chemical processing and mechanical compression.By varying the ambient humidity,the produced cellulose membranes can provide a variety of predetermined deformations,such as curve,s-like deformation and curl.The high humidity responsivity is originated from a self-maintained moisture gradient induced by an asymmetrical design of membrane surfaces,aided by the hygroscopic swelling of the cellulose.The moisture-driven actuators are then demonstrated as a three-finger gripper that can grab,hold and release objects 40 times the weight of its own.The combination of natural wood and stimuli-responsive behavior open a way to designing smart structures,actuators and soft robots with environmentally friendly,recyclable and biocompatible materials. | Xiaofeng Jiang Bingkun Tian Xiaoyu Xuan Wanqi Zhou Jianxin Zhou Yaqing Chen Yang Lu Zhuhua Zhang | 2021 | International Journal of Smart and Nano Materials2021，12，2： | 1 |
| 11 | Effects of In doping on the properties of CdZnTe single crystals显示文摘 | Yang Ge Jie Wanqi Li Qiang | | 0，，3： | 1 |
| 12 | Transcriptome profiling reveals phase-specific gene expression in the developing barley inflorescence显示文摘The shape of an inflorescence varies among cereals,ranging from a highly branched panicle in rice to a much more compact spike in barley(Hordeum vulgare L.)and wheat(Triticum aestivum L.).However,little is known about the molecular basis of cereal inflorescence architecture.We profiled transcriptomes at three developmental stages of the barley main shoot apex—spikelet initiation,floral organ differentiation,and floral organ growth—and compared them with those from vegetative seedling tissue.Transcript analyses identified 3688 genes differentially transcribed between the three meristem stages,with a further 1394 genes preferentially expressed in reproductive compared with vegetative tissue.Coexpression assembly and Gene Ontology analysis classified these 4888 genes into 28 clusters,revealing distinct patterns for genes such as transcription factors,histone modification,and cell-cycle progression specific for each stage of inflorescence development.We also compared expression patterns of VRS(SIX-ROWED SPIKE)genes and auxin-,gibberellic acid-and cytokinin-associated genes between two-rowed and six-rowed barley to describe regulators of lateral spikelet fertility.Our findings reveal barley inflorescence phase-specific gene expression,identify new candidate genes that regulate barley meristem activities and flower development,and provide a new genetic resource for further dissection of the molecular mechanisms of spike development. | Huiran Liu Gang Li Xiujuan Yang Hendrik N.J.Kuijer Wanqi Liang Dabing Zhang | 2020 | The Crop Journal2020，8，1： | 1 |
| 13 | Oral immunization of mice with plant - derived fimbrial adhesin FaeG induces systemic and mucosal K88ad enterotoxigenic Eseherichia coli-specific immune responses显示文摘 | Wanqi Liang Yahong Huang Xinghong Yang | 2006 | Medical Microbiology2006，46，3： | 1 |
| 14 | Effect of the precipitation state on high temperature tensile and creep behaviors of Mg-15Gd alloy显示文摘Due to the effective precipitation strengthening effect of the β phase, Mg-Gd alloys exhibit excellent room temperature mechanical behaviors. However, when served at high temperatures, the metastable β phase will transform to other phases, resulting in severe performance degradation. In this study, we investigated the effect of precipitation state achieved by different heat treatments on high temperature tensile and creep behaviors of the Mg-15Gd alloy by comparing the properties of the as-cast, solid-solutioned(T4) and peak-aged(T6) alloys. The results showed that the tensile mechanical properties of the T6 alloy were always highest from room temperature to 300 ℃, in spite of an abnormal strength increase with temperature existed in the T4 alloy. For tensile creep properties, the T6 alloy exhibited the lowest steady creep rate below 235 ℃ while the T4 alloy possessed the best properties above 260 ℃. Microstructure characterization revealed that the transition was caused by the stress-promoted precipitation of β phase in the T4 alloy and rapid phase transformation in the T6 alloy at high temperatures. At 260 ℃, the calculated stress exponent n was 3.1 and 2.8 for the T4 and T6 alloys, respectively, suggesting the creep deformation mechanism was dislocation slip, which was further confirmed by the microstructure after creeping. Our findings can provide new insights into the heat treatment process of Mg-Gd alloys served at high temperatures. | Shuxia Ouyang Guangyu Yang He Qin Chunhui Wang Shifeng Luo Wanqi Jie | 2022 | Journal of Magnesium and Alloys2022，10，12： | 1 |
| 15 | Electromagnetic wave absorption performance of Ti_(2)O_(3)and vacancy enhancement effective bandwidth显示文摘The microwave absorption properties of Ti_(2)O_(3)were systematically investigated.Experimental results indicate that Ti_(2)O_(3)has microwave absorption performance with a minimum reflection loss value of-37.6 d B at 18.6 GHz and an effective absorption bandwidth(RL<-10 d B)of 2 GHz.Further,vacancy defects were introduced into Ti_(2)O_(3)by carbothermal reduction.Interestingly,the effective absorption bandwidth of Ti_(2)O_(3)with vacancy defects reach 3.2 GHz.First-principles calculations provide evidence that vacancy defects result in the changes of electric dipole state,leading to a wider effective absorption bandwidth.These results have significance in understanding the origin of electromagnetic phenomena and developing electromagnetic wave absorption materials. | Xiankai Fu Bo Yang Wanqi Chen Zongbin Li Haile Yan Xiang Zhao Liang Zuo | 2021 | Journal of Materials Science & Technology2021，，17： | 0 |
| 16 | 基于巯基反应性多重定量技术的亲电性天然产物靶点发现显示文摘文章简介天然产物是新药开发和化学生物学研究的重要来源。天然产物化学结构上常见一些亲电性基团如迈克尔反应受体、环氧。这类具有亲电性的天然产物能够通过共价修饰特定功能半胱氨酸残基上的亲核性巯基基团,从而影响其所在蛋白质的功能,并且最终发挥生物学效应。 | Caiping Tian Rui Sun Keke Liu Ling Fu Xiaoyu Liu Wanqi Zhou Yong Yang 杨靖 | 2018 | 科学新闻2018，0，4： | 0 |
| 17 | Simulation research on control algorithm of differential pressure casting process显示文摘To improve the precision of the filling pressure curve of differential pressure casting controlled with PID controller,the model of differential pressure casting process is established and two pressure-difference control systems using PID algorithm and Dahlin algorithm are separately designed in MATLAB. The scheduled pressure curves controlled with PID algorithm and Dahlin algorithm,respectively,are comparatively simulated in MATLAB. The simulated pressure curves obtained show that the control precision with Dahlin algorithm is higher than that with PID algorithm in the differential pressure casting process,and it was further verified by production practice. | Chai Yan Jie Wanqi Yang Bo | 2009 | China Foundry2009，6，3： | 0 |
| 18 | An efficient method to engage oxide ceramics in low-temperature interfacial reactions: Microstructure evolution and kinetics behaviors based on supercooling in a transient liquid phase bonding joint显示文摘Ductile transient liquid phase(TLP)bonding joints reinforced by multiple precipitates were produced using novel pre-sintered coatings and Au-Si fillers;therefore,the highest strength of NiTi/sapphire joints brazed at 460℃ for 30 min reached 72 MPa.The pre-sintering process improved the surface-active of sapphire by forming metastable Ti_(3)O and non-stoichiometric Al_(2)O_(3).The typical brazing seam consisted of O-rich compounds,TiSi_(2),and Ti-Ni-Si,wherein the O-rich phase featured different crystallinity depending on the oxygen content.The sapphire/seam interface was either a nanoscale diffusion region or a Si-rich amorphous layer.The breakdown of the Stokes-Einstein relation(SER)occurred,and the deviation from SER increased with a higher cooling rate.The influence of coating thickness was reflected in(i)the supercooling related to the viscosity and fractional exponent of liquids and(ii)the microstructural change of the joint related to the driving force for crystal growth.This work presented a new strategy for joining ceramics to metals at lower temperatures but using the joint at higher temperatures;furthermore,gave an insight into the microstructure evolution and kinetics behaviors based on supercooling in a transient liquid phase bonding joint. | Jia Yang Wanqi Zhao Panpan Lin Qiuguang Zhang Xinfei Zhang Tiesong Lin Peng He Yanli Zhuang | 2023 | Journal of Materials Science & Technology2023，，20： | 0 |
| 19 | Metal–organic framework wafer enabled fast response radiation detection with ultra-low dark current显示文摘Semiconductive metal–organic frameworks(MOFs)have attracted great interest for the electronic applications.However,dark currents of present hybrid organic–inorganic materials are 1000–10,000 times higher than those of commercial inorganic detectors,leading to poor charge transportation.Here,we demonstrate a ZIF-8(Zn(mim)_(2),mim=2-methylimidazolate)wafer with ultra-low dark current of 1.27 pA·mm^(-2) under high electric fields of 322 V·mm^(-1).The isostatic pressing preparation process provides ZIF-8 wafers with good transmittance.Besides,the presence of redox-active metals and small spatial separation between components promotes the charge hopping.The ZIF-8-based semiconductor detector shows promising X-ray detection sensitivity of 70.82μC·Gy^(-1)·cm^(-2) with low doses exposures,contributing to superior X-ray imaging capability with a relatively high spatial resolution of 1.2 lp·mm^(-1).Simultaneously,good peak discrimination with the energy resolution of~43.78%is disclosed when the detector is illuminated by uncollimated 241Am@5.48 MeVα-particles.These results provide a broad prospect of MOFs for future radiation detection applications. | Meng Xu Jianxi Liu Wei Wu Yang Chen Donghao Ma Sixin Chen Wanqi Jie Menghua Zhu Yadong Xu | 2024 | Nano Research2024，17，4： | 0 |
| 20 | Gene characterization and phylogenetic analysis of four mitochondrial genomes in Caenogastropoda显示文摘Caenogastropoda is a highly diverse group,containing~60%of all existing gastropods.Species in this subclass predominantly inhabit marine environments and have a high ecological and economic value.Owing to the increase in relevant phylogenetic studies,our understanding of between species relatedness in Caenogastropoda has improved.However,the biodiversity,taxonomic status,and phylogenetic relationships of this group remain unclear.In the present study,we performed next-generation sequencing of four complete mitochondrial genomes from three families(Buccinidae,Columbellidae,and Cypraeidae)and the four mitogenomes were classical circular structures,with a length of 16177 bp in Volutharpa ampullacea,16244 bp in Mitrella albuginosa,16926bp in Mauritia arabica asiatica and 15422 bp in Erronea errones.Base composition analysis indicated that whole sequences were biased toward A and T.Then compared them with 171 complete mitochondrial genomes of Caenogastropoda.The phylogenetic relationship of Caenogastropoda derived from Maximum Likelihood(ML)and Bayesian Inference(BI)trees constructed based on CDS sequences was consistent with the results of traditional morphological analysis,with all three families showing close relationships.This study supported Caenogastropoda at the molecular level as a separate clade of Mollusca.According to our divergence time estimations,Caenogastropoda was formed during the middle Triassic period(~247.2–237 Ma).Our novel mitochondrial genomes provide evidence for the speciation of Caenogastropoda in addition to elucidating the mitochondrial genomic evolution of this subclass. | Jiangyong Qu Wanqi Yang Xindong Teng Li Xu Dachuan Zhang Zhikai Xing Shuang Wang Xiumei Liu Lijun Wang Xumin Wang | 2024 | Acta Oceanologica Sinica2024，43，2： | 0 |
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