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| 1 | LAG-3 expression on tumor-infiltrating T cells in soft tissue sarcoma correlates with poor survival显示文摘Objective: To elucidate the role and prognostic significance of lymphocyte activation-gene-3(LAG-3) in soft tissue sarcoma(STS).Methods: The expression of LAG-3 in patient and matched normal blood samples was analyzed by flow cytometry. The localization and prognostic values of LAG-3^+ cells in 163 STS patients were analyzed by immunohistochemistry. In addition, the expression of tumor-infiltrating CD3^+ T, CD4^+ T, and CD8^+ T cells and their role in the prognosis of STS were evaluated by immunohistochemistry. The effect of LAG-3 blockade was evaluated in an immunocompetent MCA205 fibrosarcoma mouse model.Results: Peripheral CD8^+ and CD4^+ T cells from STS patients expressed higher levels of LAG-3 than those from healthy donors.LAG-3 expression in STS was significantly associated with a poor clinical outcome(P = 0.038) and was correlated with high pathological grade(P < 0.001), advanced tumor stage(P = 0.016). Additionally, LAG-3 expression was highly correlated with CD8^+ T-cell infiltration(r = 0.7034, P < 0.001). LAG-3 was expressed in murine tumor-infiltrating lymphocytes, and its blockade decreased tumor growth and enhanced secretion of interferon-gamma by CD8^+ and CD4^+ T cells.Conclusions: LAG-3 blockade may be a promising strategy to improve the effects of targeted therapy in STS. | Yi Que Zhixin Fang Yuanxiang Guan Wei Xiao Bushu Xu Jingjing Zhao Huoying Chen Xinke Zhang Musheng Zeng Yao Liang Xing Zhang | 2019 | Cancer Biology & Medicine2019，16，2： | 9 |
| 2 | The brain morphology of Homo Liujiang cranium fossil by three-dimensional computed tomography显示文摘The Liujiang cranium is the most complete and well-preserved late Pleistocene human fossils ever unearthed in south China. Because the endocranial cavity is filled with hard stone matrix, earlier stud- ies focused only on the exterior morphology of the specimen using the traditional methods. In order to derive more information for the phyletic evaluation of the Liujiang cranium, high-resolution industrial computed tomography (CT) was used to scan the fossil, and the three-dimensional (3D) brain image was reconstructed. Compared with the endocasts of the hominin fossils (Hexian, Zhoukoudian, KNM-WT 15000, Sm 3, Kabwe, Brunn 3, Predmost) and modern Chinese, most morphological features of the Liujiang brain are in common with modern humans, including a round brain shape, bulged and wide frontal lobes, an enlarged brain height, a full orbital margin and long parietal lobes. A few differ- ences exist between Liujiang and the modern Chinese in our sample, including a strong posterior pro- jection of the occipital lobes, and a reduced cerebellar lobe. The measurement of the virtual endocast shows that the endocranial capacity of Liujiang is 1567 cc, which is in the range of Late Homo sapiens and much beyond the mean of modern humans. The brain morphology of Liujiang is assigned to Late Homo sapiens. | WU XiuJie LIU Wu DONG Wei QUE JieMin WANG YanFang | 2008 | Chinese Science Bulletin2008，53，16： | 8 |
| 3 | Psychological impact of the COVID-19 pandemic on healthcare workers:a cross-sectional study in China显示文摘Background Healthcare workers fighting against the coronavirus disease 2019(COVID-19)pandemic are under tremendous pressure,which puts them at an increased risk of developing psychological problems.Aims This study aimed to investigate the prevalence of psychological problems in different healthcare workers(ie,physicians,medical residents,nurses,technicians and public health professionals)during the COVID-19 pandemic in China and explore factors that are associated with the onset of psychological problems in this population during this public health crisis.Methods A cross-sectional,web-based survey was conducted in February 2020 among healthcare workers during the COVID-19 pandemic.Psychological problems were assessed using the Generalized Anxiety Disorder Scale,Patient Health Questionnaire and Insomnia Severity Index.Logistic regression analyses were used to explore the factors that were associated with psychological problems.Results The prevalence of symptoms of anxiety,depression,insomnia and the overall psychological problems in healthcare workers during the COVID-19 pandemic in China was 46.04%,44.37%,28.75%and 56.59%,respectively.The prevalence of the overall psychological problems in physicians,medical residents,nurses,technicians and public health professionals was 60.35%,50.82%,62.02%,57.54%and 62.40%,respectively.Compared with healthcare workers who did not participate in front-line work,front-line healthcare workers had a higher risk of anxiety,insomnia and overall psychological problems.In addition,attention to negative or neutral information about the pandemic,receiving negative feedback from families and friends who joined front-line work,and unwillingness to join front-line work if given a free choice were three major factors for these psychological problems.Conclusions Psychological problems are pervasive among healthcare workers during the COVID-19 pandemic.Receiving negative information and participating in front-line work appear to be.important risk factors for psychological problems.The psychological health of different healthcare workers should be protected during the COVID-19 pandemic with timely interventions and proper information feedback. | Jianyu Que Le Shi Jiahui Deng Jiajia Liu Li Zhang Suying Wu Yimiao Gong Weizhen Huang Kai Yuan Wei Yan Yankun Sun Maosheng Ran Yanping Bao Lin Lu | 2020 | General Psychiatry2020，33，3： | 8 |
| 4 | Gangba sheep in the Tibetan plateau:Validating their unique meat quality and grazing factor analysis显示文摘Gangba sheep are known for having typical sensory characteristics attributed to free range conditions and grazing on wild plants.The genuine Gangba mutton was selected as the experimental group,and the commercial Tibetan mutton was selected as the control group,the nutritive composition of basic chemical components,amino acids and fatty acids in mutton were investigated to correlate its unique meat quality and eating satisfaction.The results showed that fatty acids were significantly higher(P<0.05)in Gangba mutton than in commercial mutton,and the higher content of flavoring amino acids(glutamic acid and aspartame)were primarily responsible for the taste attributes umami of meat juices.Moreover,the trace elements analysis in mutton and grazing factors(forage,water source and soil)were conducted,to explain the source of essential trace elements in mutton.The concentrations of essential trace elements show that the Gangba mutton was a valuable source for highly available Cu and Zn in human nutrition,and well managed with few detected of toxicity metal.The concentrations of essential trace elements in mutton are closely related to the trace elements in environmental grazing factors.In conclusion,the congenital grazing conditions(a highly mineralized water resource,natural forages and clean soils)were shown to contribute to the unique meat characteristics of Gangba sheep. | Qiangying Zhang Ming Que Wei Li Shuang Gao Xin Tan Duo Bu | 2021 | Journal of Environmental Sciences2021，33，3： | 8 |
| 5 | Delivery room resuscitation and short-term outcomes of extremely preterm and extremely low birth weight infants: a multicenter survey in North China显示文摘Background:Delivery room resuscitation assists preterm infants,especially extremely preterm infants(EPI)and extremely low birth weight infants(ELBWI),in breathing support,while it potentially exerts a negative impact on the lungs and outcomes of preterm infants.This study aimed to assess delivery room resuscitation and discharge outcomes of EPI and ELBWI in China.Methods:The clinical data of EPI(gestational age[GA]<28 weeks)and ELBWI(birth weight[BW]<1000 g),admitted within 72 h of birth in 33 neonatal intensive care units from five provinces and cities in North China between 2017 and 2018,were analyzed.The primary outcomes were delivery room resuscitation and risk factors for delivery room intubation(DRI).The secondary outcomes were survival rates,incidence of bronchopulmonary dysplasia(BPD),and risk factors for BPD.Results:A cohort of 952 preterm infants were enrolled.The incidence of DRI,chest compressions,and administration of epinephrine was 55.9%(532/952),12.5%(119/952),and 7.0%(67/952),respectively.Multivariate analysis revealed that the risk factors for DRI were GA<28 weeks(odds ratio[OR],3.147;95%confidence interval[CI],2.082–4.755),BW<1000 g(OR,2.240;95%CI,1.606–3.125),and antepartum infection(OR,1.429;95%CI,1.044–1.956).The survival rate was 65.9%(627/952)and was dependent on GA.The rate of BPD was 29.3%(181/627).Multivariate analysis showed that the risk factors for BPD were male(OR,1.603;95% CI,1.061–2.424),DRI(OR,2.094;95% CI,1.328–3.303),respiratory distress syndrome exposed to≥2 doses of pulmonary surfactants(PS;OR,2.700;95%CI,1.679–4.343),and mechanical ventilation≥7 days(OR,4.358;95% CI,2.777–6.837).However,a larger BW(OR,0.998;95% CI,0.996–0.999),antenatal steroid(OR,0.577;95%CI,0.379–0.880),and PS use in the delivery room(OR,0.273;95%CI,0.160–0.467)were preventive factors for BPD(all P<0.05).Conclusion:Improving delivery room resuscitation and management of respiratory complications are imperative during early management of the health of EPI and ELBWI. | Shuai-Jun Li Qi Feng Xiu-Ying Tian Ying Zhou Yong Ji Yue-Mei Li Shu-Fen Zhai Wei Guo Fang Zhang Rong-Xiu Zheng Hai-Ying He Xia Liu Jun-Yi Wang Hua Mei Hong-Yun Wang Hua Xie Chao-Mei Zeng Li Ma Ping-Ping Zhang Jin-Yu Li Xiao-Ying Wang Li-Hua Li Hong Cui Shu-Lan Yang Lu Chen Xiao-Hong Gu Yan-Ju Hu Sheng-Shun Que Li-Xia Sun Ming Yang Wen-Li Zhao Qiu-Yan Ma Hai-Juan Wang Jiu-Ye Guo | 2021 | Chinese Medical Journal2021，，13： | 7 |
| 6 | A simple and versatile protocol for isolation of RNA from plant, fungi and animal显示文摘 | QUE You-xiong LI Wei XU Jing-sheng XU Li-ping ZHANG Mu-qing | 2008 | Journal of Agricultural Science and Technology2008，2，1： | 5 |
| 7 | Microstructure, texture and tensile properties of Mg-10Sn alloys extruded in different conditions显示文摘Indirect extrusion of Mg-10%Sn (mass fraction) alloys was performed at three different working temperatures. The effect of working temperature on the microstructure, texture and tensile properties of the extruded alloys was investigated by optical microscope (OM), scanning electronic microscope (SEM), X-ray diffraction (XRD) and a standard universal testing machine. Grain size, area fraction of second phase particles and texture of the alloys are found to be significantly influenced by working temperature. The grain size refinement is greatly dependent on processing conditions with the low working temperature being the most effective. While the high working temperature results in a coarser grain size and a stronger fiber texture and the reason for this phenomenon was examined in terms of second phase particle, grain type and dynamic recrystallization mechanism. Tested in the different conditions, the tensile strengths of the Mg-10Sn alloys extruded at the high working temperature are remarkably better than those of the other studied alloys. This significant improvement in tensile properties is mainly due to the particle strengthening and texture strengthening resulted from the more and finer primary dispersed particles and stronger texture, respectively. | CHENG Wei-li LI Jia-wei QUE Zhong-ping ZHANG Jin-shan XU Chun-xiang LIANG Wei YOU Bong-Sun PARK Sung Soo | 2013 | Journal of Central South University2013，20，7： | 2 |
| 8 | Histones released by NETosis enhance the infectivity of SARS-CoV-2 by bridging the spike protein subunit 2 and sialic acid on host cells显示文摘Neutrophil extracellular traps(NETs)can capture and kill viruses,such as influenza viruses,human immunodeficiency virus(HIV),and respiratory syncytial virus(RSV),thus contributing to host defense.Contrary to our expectation,we show here that the histones released by NETosis enhance the infectivity of SARS-CoV-2,as found by using live SARS-CoV-2 and two pseudovirus systems as well as a mouse model.The histone H3 or H4 selectively binds to subunit 2 of the spike(S)protein,as shown by a biochemical binding assay,surface plasmon resonance and binding energy calculation as well as the construction of a mutant S protein by replacing four acidic amino acids.Sialic acid on the host cell surface is the key molecule to which histones bridge subunit 2 of the S protein.Moreover,histones enhance cell-cell fusion.Finally,treatment with an inhibitor of NETosis,histone H3 or H4,or sialic acid notably affected the levels of sgRNA copies and the number of apoptotic cells in a mouse model.These findings suggest that SARS-CoV-2 could hijack histones from neutrophil NETosis to promote its host cell attachment and entry process and may be important in exploring pathogenesis and possible strategies to develop new effective therapies for COVID-19. | Weiqi Hong Jingyun Yang Jun Zou Zhenfei Bi Cai He Hong Lei Xuemei He Xue Li Aqu Alu Wenyan Ren Zeng Wang Xiaohua Jiang Kunhong Zhong Guowen Jia Yun Yang Wenhai Yu Qing Huang Mengli Yang Yanan Zhou Yuan Zhao Dexuan Kuang Junbin Wang Haixuan Wang Siyuan Chen Min Luo Ziqi Zhang Tiangi Lu Li Chen Haiying Que Zhiyao He Qiu Sun Wei Wang Guobo Shen Guangwen Lu Zhiwei Zhao Li Yang Jinliang Yang Zhenling Wang Jiong Li Xiangrong Song Lunzhi Dai Chong Chen Jia Geng Maling Gou Lu Chen Haohao Dong Yong Peng Canhua Huang Zhiyong Qian Wei Cheng Changfa Fan Yuquan Wei Zhaoming Su Aiping Tong Shuaiyao Lu Xiaozhong Peng Xiawei Wei | 2022 | Cellular & Molecular Immunology2022，19，5： | 2 |
| 9 | Genome Size of Alexandrium catenella and Gracilariopsis lemaneiformis Estimated by Flow Cytometry显示文摘Flow cytometry(FCM) technique has been widely applied to estimating the genome size of various higher plants. However, there is few report about its application in algae. In this study, an optimized procedure of FCM was exploited to estimate the genome size of two eukaryotic algae. For analyzing Alexandrium catenella, an important red tide species, the whole cell instead of isolated nucleus was studied, and chicken erythrocytes were used as an internal reference. The genome size of A. catenella was estimated to be 56.48 ± 4.14 Gb(1C), approximately nineteen times larger than that of human genome. For analyzing Gracilariopsis lemaneiformis, an important economical red alga, the purified nucleus was employed, and Arabidopsis thaliana and Chondrus crispus were used as internal references, respectively. The genome size of Gp. lemaneiformis was 97.35 ± 2.58 Mb(1C) and 112.73 ± 14.00 Mb(1C), respectively, depending on the different internal references. The results of this research will promote the related studies on the genomics and evolution of these two species. | DU Qingwei SUI Zhenghong CHANG Lianpeng WEI Huihui LIU Yuan MI Ping SHANG Erlei Zeeshan Niaz QUE Zhou | 2016 | Journal of Ocean University of China2016，15，4： | 2 |
| 10 | Efficacy,safety and immunogenicity of hexavalent rotavirus vaccine in Chinese infants显示文摘A randomized,double-blind,placebo-controlled multicenter trial was conducted in healthy Chinese infants to assess the efficacy and safety of a hexavalent live human-bovine reassortant rotavirus vaccine(HRV)against rotavirus gastroenteritis(RVGE).A total of 6400 participants aged 6-12 weeks were enrolled and randomly assigned to either HRV(n?3200)or placebo(n?3200)group.All the subjects received three oral doses of vaccine four weeks apart.The vaccine efficacy(VE)against RVGE caused by rotavirus serotypes contained in HRV was evaluated from 14 days after three doses of administration up until the end of the second rotavirus season.VE against severe RVGE,VE against RVGE hospitalization caused by serotypes contained in HRV,and VE against RVGE,severe RVGE,and RVGE hospitalization caused by natural infection of any serotype of rotavirus were also investigated.All adverse events(AEs)were collected for 30 days after each dose.Serious AEs(SAEs)and intussusception cases were collected during the entire study.Our data showed that VE against RVGE caused by serotypes contained in HRV was 69.21%(95%CI:53.31-79.69).VE against severe RVGE and RVGE hospitalization caused by serotypes contained in HRV were 91.36%(95%CI:78.45-96.53)and 89.21%(95%CI:64.51-96.72)respectively.VE against RVGE,severe RVGE,and RVGE hospitalization caused by natural infection of any serotype of rotavirus were 62.88%(95%CI:49.11-72.92),85.51%(95%CI:72.74-92.30)and 83.68%(95%CI:61.34-93.11).Incidences of AEs from the first dose to one month post the third dose in HRV and placebo groups were comparable.There was no significant difference in incidences of SAEs in HRV and placebo groups.This study shows that this hexavalent reassortant rotavirus vaccine is an effective,well-tolerated,and safe vaccine for Chinese infants. | Zhiwei Wu Qingliang Li Yan Liu Huakun Lv Zhaojun Mo Fangjun Li Qingchuan Yu Fei Jin Wei Chen Yong Zhang Teng Huang Xiaosong Hu Wei Xia Jiamei Gao Haisong Zhou Xuan Bai Yueyue Liu Zhenzhen Liang Zhijun Jiang Yingping Chen Jiuwei Zhang Jialiang Du Biao Yang Bo Xing Yantao Xing Ben Dong Qinghai Yang Chen Shi Tingdong Yan Bo Ruan Haiyun Shi Xingliang Fan Dongyang Feng Weigang Lv Dong Zhang Xiangchu Kong Liuyifan Zhou Dinghong Que Hong Chen Zhongbing Chen Xiang Guo Weiwei Zhou Cong Wu Qingrong Zhou Yuqing Liu Jian Qiao Ying Wang Xinguo Li Kai Duan Yuliang Zhao Gelin Xu Xiaoming Yang | 2022 | Virologica Sinica2022，37，5： | 2 |
| 11 | Transcriptional regulation of oil biosynthesis inseed plants: Current understanding, applications,and perspectives显示文摘Plants produce and accumulate triacylglycerol(TAG)in their seeds as an energy reservoir to support the processes of seed germination and seedling development.Plant seed oils are vital not only for the human diet but also as renewable feedstocks for industrial use.TAG biosynthesis consists of two major steps:de novo fatty acid biosynthesis in the plastids and TAG assembly in the endoplasmic reticulum.The latest advances in unraveling transcriptional regulation have shed light on the molecular mechanisms of plant oil biosynthesis.We summarize recent progress in understanding the regulatory mechanisms of wellcharacterized and newly discovered transcription factors and other types of regulators that control plant fatty acid biosynthesis.The emerging picture shows that plant oil biosynthesis responds to developmental and environmental cues that stimulate a network of interacting transcriptional activators and repressors,which in turn fine-tune the spatiotemporal regulation of the pathway genes. | Yuzhou Yang Que Kong Audrey R.Q.Lim Shaoping Lu Hu Zhao Liang Guo Ling Yuan Wei Ma | 2022 | Plant Communications2022，3，5： | 2 |
| 12 | Varicella-zoster virus ORF7 interacts with ORF53 and plays a role in its trans-Golgi network localization显示文摘Varicella-zoster virus(VZV) is a neurotropic alphaherpesvirus that causes chickenpox and shingles. ORF7 is an important virulence determinant of VZV in both human skin and nerve tissues,however, its specific function and involved molecular mechanism in VZV pathogenesis remain largely elusive. Previous yeast two-hybrid studies on intraviral protein-protein interaction network in herpesviruses have revealed that VZV ORF7 may interact with ORF53, which is a virtually unstudied but essential viral protein. The aim of this study is to identify and characterize VZV ORF53, and to investigate its relationship with ORF7. For this purpose, we prepared monoclonal antibodies against ORF53 and, for the first time, characterized it as a ~40 k Da viral protein predominantly localizing to the trans-Golgi network of the infected host cell. Next, we further confirmed the interaction between ORF7 and ORF53 by co-immunoprecipitation and co-localization studies in both plasmid-transfected and VZV-infected cells. Moreover, interestingly, we found that ORF53 lost its trans-Golgi network localization and became dispersed in the cytoplasm of host cells infected with an ORF7-deleted recombinant VZV, and thus ORF7 seems to play a role in normal subcellular localization of ORF53. Collectively, these results suggested that ORF7 and ORF53 may function as a complex during infection, which may be implicated in VZV pathogenesis. | Wei Wang Wenkun Fu Dequan Pan Linli Cai Jianghui Ye Jian Liu Che Liu Yuqiong Que Ningshao Xia Hua Zhu Tong Cheng | 2017 | Virologica Sinica2017，32，5： | 1 |
| 13 | Enhanced bioavailability of silymarin by self-microemulsifying drug delivery system显示文摘 | Wei Wu Yang Wang Li Que | 2006 | Eur J Pharm Biopharm2006，63，3： | 1 |
| 14 | Evaluation of disintegrating time of rapidly disintegrating tablets by a paddle method 显示文摘 | Li Que Wei Wu Xiao Fengcheng | 2006 | Pharm Dev Technol2006，11，3： | 1 |
| 15 | Temperature compensa- tion for thermal anemometers using temperature sensors inde- pendent of flow sensors 显示文摘 | QUE R Y ZHU R WEI Q Z | 2011 | Measurement Science and Tech- nology2011，22，08： | 1 |
| 16 | Evaluation of Disintegration Time of Rapidly Disintegrating Tablets by a Paddle Method显示文摘 | Li Que Wei Wu Xiao Feng Cheng | 2006 | Pharmaceutical Development and Technology2006，11，： | 1 |
| 17 | Evaluation of disintegra ting time of rapidly disintegrating tablets by a paddle method 显示文摘 | Li Que Wei Wu Xiao Fengcheng | 2006 | Pharm Dcv Techrwl2006，11，3： | 1 |
| 18 | Central pancreatectomy for benign or low-grade malignant pancreatic tumors in the neck and body of the pancreas显示文摘BACKGROUND For tumors in the neck and body of the pancreas,distal pancreatectomy(DP)has been the standard surgical procedure for the last few decades and central pancreatectomy(CP)is an alternative surgical option.Whether CP better preserves remnant pancreatic endocrine and exocrine functions after surgery remains a subject of debate.AIM To evaluate the safety and efficacy of CP compared with DP for benign or lowgrade malignant pancreatic tumors in the neck and body of the pancreas.METHODS This retrospective study enrolled 296 patients who underwent CP or DP for benign and low-malignant neoplasms at the same hospital between January 2016 and March 2020.Perioperative outcomes and long-term morbidity of endocrine/exocrine function were prospectively evaluated.RESULTS No significant difference was observed in overall morbidity or clinically relevant postoperative pancreatic fistula between the two groups(P=0.055).Delayed gastric emptying occurred more frequently in the CP group than in the DP group(29.4%vs 15.3%;P<0.005).None of the patients in the CP group had new-onset or aggravated distal metastasis,whereas 40 patients in the DP group had endocrine function deficiency after surgery(P<0.05).There was no significant difference in the incidence of diarrhea immediately after surgery,but at postoperative 12 mo,a significantly higher number of patients had diarrhea in the DP group than in the CP group(0%vs 9.5%;P<0.05).CONCLUSION CP is a generally safe procedure and is better than DP in preserving long-term pancreatic endocrine and exocrine functions.Therefore,CP might be a better option for treating benign or low-grade malignant neoplasms in suitable patients. | Yi-Wen Chen Jian Xu Xiang Li Wei Chen Shun-Liang Gao Yan Shen Min Zhang Jian Wu Ri-Sheng Que Jun Yu Ting-Bo Liang Xue-Li Bai | 2022 | World Journal of Gastrointestinal Surgery2022，14，9： | 1 |
| 19 | SARS-CoV-2 variants,immune escape,COVID-19 vaccine,and therapeutic strategies显示文摘The global pandemic of coronavirus disease 2019(COVID-19) caused by severe acute respiratory syndrome coronavirus2(SARS-CoV-2) has brought considerable challenges to worldwide public health and the economy. Over 535 million confirmed cases and 6.3 million deaths of COVID-19 were reported as of June 17, 2022(http://gffzze7c39cdc9e894ca6spx6cqc5pxcwx6nkc.ffgz.tsg.suse.edu.cn/). | Haiying Que Li Chen Xiawei Wei | 2023 | Science China(Life Sciences)2023，66，2： | 1 |
| 20 | Sparse deconvolution method for improving the time-resolution of ultrasonic NDE signals显示文摘 | Wei Hang Huang Zuo-ying Que Pei-wenb | 2009 | NDT&E International2009，，42： | 1 |
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