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| 1 | The Genome of Artemisia annua Provides Insight into the Evolution of Asteraceae Family and Artemisinin Biosynthesis显示文摘Artemisia annua,通常已知的同样香甜的苦恼或 Qinghao,是到中国的一个灌木土著人并且长被用于药用的目的。A。annua 现在作为有势力抗疟药混合物的唯一的生来的来源全球性被栽培, artemisinin。这里,我们报导 A 的 1.74-gigabase 染色体的一个高质量的草稿集会。annua,高度异质接合,富于重复定序,并且包含 63 ? 226 编码蛋白质的基因,在定序的植物种类之中的最大的数字之一。我们发现了那,作为在 Asteraceae 的一些定序的染色体之一, A。annua 染色体包含对这大被子植物 clade 特定的很多基因。尤其是,扩大和编码涉及萜烯生合成的酶的基因的功能的多样化与 artemisinin biosynthetic 小径的进化一致。我们进一步由介绍那 A 的 transcriptome 揭示了。annua 发展了复杂 transcriptional 规章的网络位于 \O 下面 artemisinin 生合成。把转基因的 A 基于我们产生了的全面 genomic 和 transcriptomic 分析。artemisinin 高级的生产的 annua 线,它现在为大规模生产准备好了并且将从而帮助遇见增加 artemisinin 的全球需求的挑战。 | Qian Shen Lida Zhang Zhihua Liao Shengyue Wang Tingxiang Yan Pu Shi Meng Liu Xueqing Fu Qifang Pan Yuliang Wang Zongyou Lv Xu Lu Fangyuan Zhang Weimin Jiang Yanan Ma Minghui Chen Xiaolong Hao Ling Li Yueli Tang Gang Lv Yan Zhou Xiaofen Sun Peter E. Brodelius Jocelyn K.C. Rose Kexuan Tang | 2018 | Molecular Plant2018，11，6： | 47 |
| 2 | Changes of net primary productivity in China during recent 11 years detected using an ecological model driven by MODIS data显示文摘网主要生产率(NPP ) 是陆上的碳周期的一个重要部件。精确地在中国印射 NPP 的空间时间的变化为全球碳骑车是关键的学习。在这研究,基于过程的北方的生态系统生产率模拟器(BEPS ) 被采用从 2000 ~ 2010 为时期在中国的生态系统学习 NPP 的变化。BEPS 模型首先用在在不同区域测量的典型流动地点和森林 NPP 测量的粗野主要生产率(GPP ) 被验证。然后,它与叶区域索引( LAI )被驾驶从中等分辨率成像分光辐射函数( MODIS )反射和陆地盖子产品和气象学的数据的 inversed 在 753 个国家基本气象学的车站从观察插入内推从1月1日在每日的时间步和 500 m 的空间分辨率模仿 NPP , 2000 到2010年12月31日。确认证明 BEPS 能在中国的不同区域捕获基于塔的 GPP 的季节的变化和森林 NPP 的空间可变性。年度 NPP 的估计的国家总数从 2.63 变化了到 2.84Pg C 獥吗? | Yibo LIU Weimin JU Honglin HE Shaoqiang WANG Rui SUN Yuandong ZHANG | 2013 | Frontiers of Earth Science2013，7，1： | 30 |
| 3 | Altered trends in carbon uptake in China’s terrestrial ecosystems under the enhanced summer monsoon and warming hiatus显示文摘The carbon budgets in terrestrial ecosystems in China are strongly coupled with climate changes.Over the past decade,China has experienced dramatic climate changes characterized by enhanced summer monsoon and decelerated warming.However,the changes in the trends of terrestrial net ecosystem production(NEP)in China under climate changes are not well documented.Here,we used three ecosystem models to simulate the spatiotemporal variations in China's NEP during 1982–2010 and quantify the contribution of the strengthened summer monsoon and warming hiatus to the NEP variations in four distinct climatic regions of the country.Our results revealed a decadal-scale shift in NEP from a downtrend of–5.95 Tg C/yr^2(reduced sink)during 1982–2000 to an uptrend of 14.22 Tg C/yr^2(enhanced sink)during 2000–10.This shift was essentially induced by the strengthened summer monsoon,which stimulated carbon uptake,and the warming hiatus,which lessened the decrease in the NEP trend.Compared to the contribution of 56.3%by the climate effect,atmospheric CO2 concentration and nitrogen deposition had relatively small contributions(8.6 and 11.3%,respectively)to the shift.In conclusion,within the context of the global-warming hiatus,the strengthening of the summer monsoon is a critical climate factor that enhances carbon uptake in China due to the asymmetric response of photosynthesis and respiration.Our study not only revealed the shift in ecosystem carbon sequestration in China in recent decades,but also provides some insight for understanding ecosystem carbon dynamics in other monsoonal areas. | Honglin He Shaoqiang Wang Li Zhang Junbang Wang Xiaoli Ren Lei Zhou Shilong Piao Hao Yan Weimin Ju Fengxue Gu Shiyong Yu Yuanhe Yang Miaomiao Wang Zhongen Niu Rcmg Ge Huimin Yan Mei Huang Guoyi Zhou Yongfei Bai Zongqiang Xie Zhiyao Tang Bingfang Wu Leiming Zhang Nianpeng He Qiufeng Wang Guirui Yu | 2019 | National Science Review2019，6，3： | 24 |
| 4 | 5-Hydroxymethylcytosine signatures in cell-free DNA provide information about tumor types and stages显示文摘5-Hydroxymethylcytosine (5hmC ) 是被连接了到基因规定和癌症致病的重要哺乳动物的 DNA epigenetic 修正。这里,我们用定序途径的一枝敏感化学基于标签的低输入的猎枪在传播没有房间的 DNA (cfDNA ) 探索了 5hmC 的诊断潜力。我们从七种不同癌症类型的 49 个病人定序没有房间的 5hmC 并且发现了能被用来与高精确性预言癌症类型和阶段的不同特征。明确地,我们发现肺癌症在 cfDNA 导致 5hmC 的进步全球损失,而 hepatocellular 癌和胰腺的癌症在没有房间的 hydroxymethylome 导致疾病特定的变化。我们的 proof-of-principle 结果建议没有房间的 5hmC 签名可以潜在地被使用不仅识别癌症类型而且在一些癌症追踪肿瘤舞台。 | Chun-Xiao Song Senlin Yin Li Ma Amanda Wheeler Yu Chen Yan Zhang Bin Liu Junjie Xiong Weihan Zhang Jiankun Hu Zongguang Zhou Biao Dong Zhiqi Tian Stefanie S Jeffrey Mei-Sze Chua Samuel So Weimin Li Yuquan Wei Jiajie Diao Dan Xie Stephen R Quake | 2017 | Cell Research2017，27，10： | 17 |
| 5 | An operational two-layer remote sensing model to estimate surface flux in regional scale: Physical background显示文摘Based on the improved interaction mechanism of two-layer model, this paper proposed Pixel Component Arranging and Comparing Algorithm (PCACA) and theoretically positioning algorithm, estimated the true temperature of mixed pixel in four extreme points in combination with the measurements of dry and wet points in calibration fields and improved the reliability of positioning dry and wet line. A new two-layer energy-separation algorithm was proposed,which was simple and direct without resistance network parameters for each pixel. We also proposed a new thought about the effect of advection. The albedo of mixed pixel was also separated with PCACA. In combination with two-layer energy-separation algorithm, the net radiation of mixed pixel was separated to overcome the uncertainty of conventional energy-separation algorithm using Beer's Law. Through the validation of retrieval result, this method is proved to be feasible and operational. At the same time, the uncertainty of this algorithm was objectively analyzed. | ZHANG Renhua SUN Xiaomin WANG Weimin XU Jinping ZHU Zhilin TIAN Jing | 2005 | Science China Earth Sciences2005，48，z1： | 16 |
| 6 | PRMT5 regulates Golgi apparatus structure through methylation of the golgin GM130显示文摘Golgi 仪器(GA ) 的维护结构和功能取决于 Golgi 矩阵蛋白质。象 golgin 和掌握家庭的成员的 phosphorylation 那样的 Golgi 蛋白质的 posttranslational 修正为决定 Golgi 建筑学是重要的。包括 golgin-84 的一些 Golgi 蛋白质也被知道是 methylated,但是 golgin methylation 的功能仍然保持不清楚。这里,我们证明蛋白质精氨酸 methyltransferase (PRMT5 ) 5 本地化到 GA 和形式建筑群,几个部件在拴住的 GA 带子形成和泡包含了。PRMT5 在 Golgi 带子形成与 PRMT5 原因缺点的 golgin GM130,和弄空交往。而且,在 GM130 的 PRMT5 甲醇化物 N 终端精氨酸,和如此的精氨酸 methylation 为 GA 显得批评带子形成。我们的调查结果揭示 GM130 的 PRMT5 依赖的精氨酸 methylation 由控制 GA 建筑学的维护的分子的机制。 | Zhongwei Zhou Xiaotian Sun Zhenhua Zou Litao Sun Tao Zhang Shaoshi Guo Ya Wen Lin Liu Yi Wang Jun Qin Lei Li Weimin Gong Shilai Bao | 2010 | Cell Research2010，20，9： | 16 |
| 7 | Preparation and self-assembly of amphiphilic polymer with aggregation-induced emission characteristics显示文摘An amphiphilic polymer bearing tetraphenylethene (TPE) moiety was synthesized by convenient reactions. The polymer exhibits unique aggregation-induced emission (AIE) characteristics and can self-assemble to size-tunable particles in DMF/water mixtures. The polymer nanoparticles can be used for cell imaging, which provides a potential stable fluorescent tool to monitor the distribution of drugs and bioconjugates in living cells. | QIN AnJun ZHANG Ya HAN Ning MEI Ju SUN JingZhi FAN WeiMin TANG Ben Zhong | 2012 | Science China Chemistry2012，55，5： | 16 |
| 8 | Chronology,Hf isotopes,geochemistry,and petrogenesis of the magmatic rocks in the Shizishan ore field of Tongling,Anhui Province显示文摘LA-ICPMS zircon U-Pb dating of intrusive rocks from the Shizishan ore field,Tongling,Anhui Province shows that the crystallization ages of Baimangshan pyroxene diorite,Shizishan and Dongguashan quartz diorite are 139±1.9,138.0±1.7,and 138.4±1.7 Ma,respectively.These three intrusions are high-potassium,alkali-rich,meta-aluminous,and are characterized by enrichment of light rare earth elements and large-ion lithophile elements,depletion of heavy rare-earth elements(HREE) and high field strength elements,and slightly negative Eu anomalies.In-situ Hf analysis of zircon for these three intrusions shows large ranges of Hf isotope compositions:the ε Hf(t) values are ε Hf(t)= 3.3 to 14.4 for Baimangshan,14.1 to 23.9 Dongguashan,and 14.7 to 26.9 for Shizishan.The ε Hf(t) values of both the Dongguashan and Shizishan quartz diorites are significantly lower than those of Baimangshan pyroxene diorite.Elemental geochemistry and in-situ zircon Hf isotope analyses indicate that the three intrusions were formed through mixing of granitic magma formed by partial melting of the lower crust and water-rich basic magma derived from the enriched lithospheric mantle.The Dongguashan and Shizishan quartz diorites involved greater crustal components than Baimangshan pyroxene diorite.The Dongguashan and Shizishan quartz diorites have geochemical features similar to the adakite,such as high Sr,low Y,and high Sr/Y ratios.Such geochemical features can be attributed to some fractional crystallization of apatite and sphene,and insignificant plagioclase fractional crystallization after magma mixing.These three intrusions are the products of complex crust-mantle interaction during the regional tectonic regime transition from compression to extension. | GUO WeiMin LU JianJun JIANG ShaoYong ZHANG RongQing ZHAO ZhanJie | 2013 | Science China Earth Sciences2013，56，6： | 15 |
| 9 | Reversal of B-cell hyperactivation and functional impairment is associated with HBsAg seroconversion in chronic hepatitis B patients显示文摘B 房间对再感染在肝炎 B 病毒(HBV ) 和保护的清理起一个重要作用。然而,与长期的 HBV 感染的结果被联系的这些房间的功能的特征仍然保持未知。我们包括地在不同阶段从 CHB 病人调查了外部 B 房间子集的频率,显型,和功能:有免疫力的忍耐(信息技术) ,有免疫力的激活(IA ) ,有免疫力的清理(IC ) ,有 HBsAg seroconversion 的应答者(解决的病人, RP ) ,并且健康控制(HC ) 。IA 病人与另外的组相比显示了外部血记忆 B 房间的更低的百分比。B 房间的全面 polyclonal 激活,由激活标记和 IgG 和 IgM 的分泌物的高水平显示了,在 IA 病人被观察。这 B 房间 hyperactivation 能被增加的 IFN-α 导致;并且在 IA 病人的可溶的 CD40 ligands。尤其是,合作激发器分子 CD80 和浆液 HBsAb 的表示和 HBsAg 特定的 B 房间的频率显著地与 HC 题目相比在信息技术, IA,和 IC 病人被减少。更重要地, B 房间 hyperactivation, co-stimulatory 分子 downregulation 和 HBsAg 特定的 B 房间缺陷在 RP 病人被颠倒。B 房间 hyperactivation 和功能的缺陷的颠倒在长期的肝炎 B 病人与 HBsAg seroconversion 被联系。 | Xiangsheng Xu Qinghua Shang Xinyue Chen Weimin Nie Zhengsheng Zou Ang Huang Ming Meng Lei Jin Ruonan Xu Ji-Yuan Zhang Junliang Fu Lifeng Wang Zirong Tang Yunbo Xie Xiaoming Yang Zheng Zhang Fu-Sheng Wang | 2015 | Cellular & Molecular Immunology2015，12，3： | 15 |
| 10 | Effect of sediment resuspension on underwater light field in shallow lakes in the middle and lower reaches of the Yangtze River:A case study in Longgan Lake and Taihu Lake显示文摘Based on three continuous in situ underwater light field measurement under different wind waves conditions in Longgan Lake, Meiliang Bay of Taihu Lake in July 2003 and littoral zone near TLLER in July 2004, respectively, the effects of sediment resuspension caused by wind waves on PAR diffuse attenuation, absorption coefficients and euphotic depths are analyzed. In Longgan Lake, PAR diffuse attenuation coefficients during small, middle and large wind waves were 1.74, 2.02 and 2.45 m-1, respectively, and the corresponding PAR spectral diffuse attenuations ranged from 0.98 to 2.97, 1.34 to 3.95 and 1.80 to 5.40 m-1, respectively. In Meiliang Bay, PAR diffuse attenuation coefficients were 2.63, 3.72, 4.37 m-1 during small, middle and large wind waves. PAR diffuse attenuation coefficients increased by 41% and 66% from small to middle, large wind waves, respectively.Absorption coefficients integrated over the range of PAR of CDOM, phytoplankton were 0.26, 0.28 m-1;0.76, 0.49 m-1, respectively during middle and large wind waves. Absorption coefficients integrated over the range of PAR of non-algal particulate matter and total suspended particulate matter increased from 0.94 to 1.73 m-1, and from 1.70 to 2.22 m-1, respectively during middle and large wind waves. Relative contributions of absorption coefficients of non-algal particulate matter to total absorption coefficient integrated over the range of PAR were 44.14%, 65.05%, respectively, during middle and large wind waves. PAR euphotic depths decreased by 0.40, 0.19, 0.20 m from middle to large wind waves in Longganhu Lake, Meliang Bay and littoral zone near TLLER. Significant correlations were found between transparency, PAR diffuse attenuation coefficients, euphotic depths and total suspended particulate matter, wind velocity, wave height. Most significant correlations were found between transparency, PAR diffuse attenuation coefficients, euphotic depths and inorganic suspended particulate matter but low correlations for chlorophyll a, dissolved organic carbon. Increase of total suspended particulate matter, especially inorganic suspended particulate matter caused by wind waves was the dominant factor affecting underwater light field in shallow lakes in the middle and lower reaches of the Yangtze River based on observations at three stations. | ZHANG Yunlin, QIN Boqiang, ZHU Guangwei, GAO Guang, LUO Liancong & CHEN Weimin Nanjing Institute of Geography & Limnology, Chinese Academy of Sciences, Nanjing 210008, China Graduate School of Chinese Academy of Sciences, Beijing 100039, China | 2006 | Science China Earth Sciences2006，49，z1： | 14 |
| 11 | TRAIL receptor mediates inflammatory cytokine release in an NF-κB-dependent manner显示文摘在现在的文章,我们报导 DR4 或 DR5 overexpression 戏剧性地在 293T, MDA-MB-231 和 HCT-116 房间以一种 NF-魏 B 依赖者方式激活煽动性的 cytokines IL-8, TNF- 伪, CCL20, MIP-2 和 MIP-1 尾的版本。我们显示出调停受体的信号是的那死亡细胞外域无关,而 DR4 细胞内部的领域的 overexpression 的效果是少得多有势力。表明串联的 TRADD-TRAF2-NIK-IKK 伪 / 尾,在导致 TNF 的 NF-魏 B 激活起一个必要作用,被发现涉及肿瘤坏死因素相关导致 apoptosis ligand (小道) 调停受体的信号 transduction。表明小径的 FADD-caspase,被报导了主要与 apoptosis 有关,作为与表明串联的 TRADD-TRAF2-NIK-IKK 伪 / 尾为 DR4 或 DR5 调停 overexpression 的 NF-魏 B 激活和 cytokine 分泌物和串音是必要的被识别。而且, DR5 论战的抗体(AD5-10 ) 触发了煽动性的 cytokine 版本。这些数据和以前的报告,提供小道和小道受体在发炎起一个重要作用的充分证据。 | Wanhu Tang Weimin Wang Yaxi Zhang Shilian Liu Yanxin Liu Dexian Zheng | 2009 | Cell Research2009，19，6： | 14 |
| 12 | Parameter estimation method based on parameter function surface显示文摘By analyzing the structure of the objective function based on error sum of squares and the information provided by the objective function, the essential problems in the current parameter estimation methods are summarized: (1) the information extracted from the objective function based on error sum of squares is unreasonable or even wrong for parameter estimation; and (2) the surface of the objective function based on error sum of squares is more complex than that of the parameter function, which indicates that the optimal parameter values should be searched on the surface of the parameter function instead of the objective function. This paper proposes the concept of sample intersection and demonstrates the uniqueness theorem of intersection point (namely the uniqueness of optimal parameter values). According to the characteristics of parameter function surface and Taylor series expansion, a parameter estimation method based on the sample intersection information extracted from parameter function surface (PFS method) was constructed. The results of theoretical analysis and practical application show that the proposed PFS method can avoid the problems in the current automatic parameter calibration, and has fast convergence rate and good performance in parameter calibration. | BAO WeiMin ZHANG XiaoQin ZHAO LiPing | 2013 | Science China(Technological Sciences)2013，56，6： | 13 |
| 13 | Re-Os isotope dating of pyrite from the footwall mineralization zone of the Xinqiao deposit,Tongling,Anhui Province:Geochronological evidence for submarine exhalative sedimentation显示文摘The Xinqiao deposit is located in the Tongling area,Anhui Province.This deposit is dominantly composed of stratiform sulfide orebodies,which are restricted to the transitional zone from clastic rocks to carbonates at the base of the Late Carboniferous Huanglong Formation.There are discordant veinlets or networks of mineralization in the footwall rocks below the stratiform sulfide orebodies.The underlying discordant mineralization zones coexist with conformable stratiform sulfide orebodies,which are considered to be the most representative features of massive sulfide deposits formed by submarine exhalative sedimentation.This study reports a pyrite 187Re-187Os isotopic age of 319±13 Ma(MSWD=16) for the footwall mineralization zone.This isochron age is consistent with the interval of strata hosting the conformable stratiform sulfide orebody,and provides direct geochronological evidence for Carboniferous exhalative sedimentary mineralization. | GUO WeiMin LU JianJun JIANG ShaoYong ZHANG RongQing QI Liang | 2011 | Chinese Science Bulletin2011，56，35： | 11 |
| 14 | Structural insights into a novel histone demethylase PHF8显示文摘histone methylation/demethylation 的动态规定在开发期间起一个重要作用。在人的植物 homeodomain (哲学博士) 的变化和截断摸蛋白质 8 (PHF8 ) 与 X 连接智力迟钝和面部异例被联系,例如一张长脸,宽广鼻音尖端,劈开 lip/cleft 腭和大手,然而,它的分子的功能和结构的基础仍然保持不清楚。这里,我们在高分辨率报导 PHF8 的催化核心的水晶结构与或没有伪 - ketoglutarate (伪 - KG ) 。生物化学、结构的研究表明 PHF8 是为甲醇化物 di 、单音甲醇化物的 histone H3 离氨酸 9,然而并非为 H3K9me3 特定的新奇 histone demethylase (H3K9me2/1 ) 。我们的分析也揭示人的 PHF8 怎么在 methylation 状态之间区别并且为 methylated H3K9 完成顺序特性。在里面 vitro demethylation 试金也证明在临床的病人观察的 F279S 异种不拥有 demethylation 活动,建议酶的活动的那损失为 PHF8 病人的致病是关键的。一起拿,这些结果将使位于联系 PHF8 的发展、神经病学的疾病下面的分子的机制清楚些。 | Lin Yu Yang wang Shuo Huang Jianjun Wang Zengqin Deng Qi Zhang Wei Wu Xingliang Zhang Zhao Liu Weimin Gong Zhongzhou Chen | 2010 | Cell Research2010，20，2： | 11 |
| 15 | A new prognostic histopathologic classification of nasopharyngeal carcinoma显示文摘Background:The current World Health Organization(WHO) classification of nasopharyngeal carcinoma(NPC) con?veys little prognostic information.This study aimed to propose an NPC histopathologic classification that can poten?tially be used to predict prognosis and treatment response.Methods:We initially developed a histopathologic classification based on the morphologic traits and cell differentia?tion of tumors of 2716 NPC patients who were identified at Sun Yat?sen University Cancer Center(SYSUCC)(training cohort).Then,the proposed classification was applied to 1702 patients(retrospective validation cohort) from hospitals outside SYSUCC and 1613 patients(prospective validation cohort) from SYSUCC.The efficacy of radiochemotherapy and radiotherapy modalities was compared between the proposed subtypes.We used Cox proportional hazards models to estimate hazard ratios(HRs) with 95% confidence intervals(CI) for overall survival(OS).Results:The 5?year OS rates for all NPC patients who were diagnosed with epithelial carcinoma(EC;3708 patients),mixed sarcomatoid?epithelial carcinoma(MSEC;1247 patients),sarcomatoid carcinoma(SC;823 patients),and squamous cell carcinoma(SCC;253 patients) were 79.4%,70.5%,59.6%,and 42.6%,respectively(P < 0.001).In mul?tivariate models,patients with MSEC had a shorter OS than patients with EC(HR = 1.44,95% CI = 1.27–1.62),SC(HR = 2.00,95% CI = 1.76–2.28),or SCC(HR = 4.23,95% CI = 3.34–5.38).Radiochemotherapy significantly improved survival compared with radiotherapy alone for patients with EC(HR 49–0.75),and possibly for those with SCC(HR = 0.67,95% CI = 0.56–0.80),MSEC(HR = 0.58,95% CI = 0..74–1.28).= 0.63;95% CI = 0.40–0.98),but not for patients with SC(HR = 0.97,95% CI = 0Conclusions:The proposed classification offers more information for the prediction of NPC prognosis compared with the WHO classification and might be a valuable tool to guide treatment decisions for subtypes that are associ?ated with a poor prognosis. | Hai-Yun Wang Yih-Leong Chang Ka-Fai To Jacqueline S.G.Hwang Hai-Qiang Mai Yan-Fen Feng Ellen T.Chang Chen-Ping Wang Michael Koon Ming Kam Shie-Lee Cheah Ming Lee Li Gao Hui-Zhong Zhang Jie-Hua He Hao Jiang Pei-Qing Ma Xiao-Dong Zhu Liang Zeng Chun-Yan Chen Gang Chen Ma-Yan Huang Sha Fu Qiong Shao An-Jia Han Hai-Gang Li Chun-Kui Shao Pei-Yu Huang Chao-Nan Qian Tai-Xiang Lu Jin-Tian Li Weimin Ye Ingemar Ernberg Ho Keung Ng Joseph T.S.Wee Yi-Xin Zeng Hans-Olov Adami Anthony T.C.Chan Jian-Yong Shao | 2016 | Chinese Journal of Cancer2016，35，6： | 10 |
| 16 | Stem cell transplantation for treatment of cerebral ischemia in rats Effects of human umbilical cord blood stem cells and human neural stem cells显示文摘BACKGROUND: Exogenous neural stem cell transplantation promotes neural regeneration. However, various types of stem cells transplantation outcomes remain controversial. OBJECTIVE: To explore distribution, proliferation and differentiation of human neural stem cells (hNSCs) and human umbilical cord blood stem cells (hUCBSCs) following transplantation in ischemic brain tissue of rats, and to compare therapeutic outcomes between hNSCs and hUCBSCs. DESIGN, TIME AND SETTING: Randomized controlled animal studies were performed at the Experimental Animal Center of Nanjing Medical University and Central Laboratory of Second Affiliated Hospital of Nanjing Medical University of China from September 2008 to April 2009. MATERIALS: hNSCs were harvested from brain tissue of 10-13 week old fetuses following spontaneous abortion, and hUCBSCs were collected from umbilical cord blood of full-term newborns at the Second Affiliated Hospital of Nanjing Medical University of China. hNSCs and hUCBSCs were labeled by 5-bromodeoxyuridine (BrdU) prior to transplantation. METHODS: Rat models of cerebral ischemia were established by the suture method. A total of 60 healthy male Sprague Dawley rats aged 7-9 weeks were randomly assigned to hNSC transplantation, hUCBSC transplantation and control groups. The rat models in the hNSC transplantation, hUCBSC transplantation and control groups were infused with hNSC suspension, hUCBSC suspension and saline via the caudal vein, respectively. MAIN OUTCOME MEASURES: The distribution, proliferation and differentiation of hNSCs and hUCBSCs in ischemic brain tissue were observed using immunohistochemical methods. Neurological function in rats was assessed using the neurological severity score. RESULTS: The number of BrdU-positive cells was significantly greater in the hNSC transplantation group compared with hUCBSC transplantation group at 14 days following transplantation (P < 0.05). The number of BrdU-positive cells reached a peak at 28 days following transplantation. Nestin-positive, glial fibrillary acidic protein-positive, cyclic nucleotide 3' phosphohydrolase-positive and neuron specific enolase-positive cells were visible following transplantation. No significant difference was determined in the constituent ratio of various cells between hNSC and hUCBSC transplantation groups (P > 0.05). The neurological severity score was significantly decreased in rats at 21 days following transplantation (P < 0.05). No significant difference was detected in neurological severity score between hNSC and hUCBSC transplantation groups at various time points (P > 0.05). CONCLUSION: The transplanted hNSCs and hUCBSCs can migrate into ischemic brain tissue, proliferate and differentiate into neuron-like, astrocyte-like and oligodendrocyte-like cells, and improve neurological function in rats with cerebral ischemia. | Fenghua Pan Xinsheng Ding Haixia Ding Weimin Zhang Xiaoxuan Deng Juan Yao | 2010 | Neural Regeneration Research2010，5，7： | 10 |
| 17 | Genome-wide association study identifies favorable SNP alleles and candidate genes for waterlogging tolerance in chrysanthemums显示文摘Chrysanthemums are sensitive to waterlogging stress,and the development of screening methods for tolerant germplasms or genes and the breeding of tolerant new varieties are of great importance in chrysanthemum breeding.To understand the genetic basis of waterlogging tolerance(WT)in chrysanthemums,we performed a genome-wide association study(GWAS)using 92,811 single nucleotide polymorphisms(SNPs)in a panel of 88 chrysanthemum accessions,including 64 spray cut and 24 disbud chrysanthemums.The results showed that the average MFVW(membership function value of waterlogging)of the disbud type(0.65)was significantly higher than that of the spray type(0.55)at P<0.05,and the MFVW of the Asian accessions(0.65)was significantly higher than that of the European accessions(0.48)at P<0.01.The GWAS performed using the general linear model(GLM)and mixed linear model(MLM)identified 137 and 14 SNP loci related to WT,respectively,and 11 associations were commonly predicted.By calculating the phenotypic effect values for 11 common SNP loci,six highly favorable SNP alleles that explained 12.85—21.85%of the phenotypic variations were identified.Furthermore,the dosage-pyramiding effects of the favorable alleles and the significant linear correlations between the numbers of highly favorable alleles and phenotypic values were identified(r2=0.45;P<0.01).A major SNP locus(Marker6619-75)was converted into a derived cleaved amplified polymorphic sequence(dCAPS)marker that cosegregated with WT with an average efficiency of 78.9%.Finally,four putative candidate genes in the WT were identified via quantitative real-time PCR(qRT-PCR).The results presented in this study provide insights for further research on WT mechanisms and the application of molecular marker-assisted selection(MAS)in chrysanthemum WT breeding programs. | Jiangshuo Su Fei Zhang Xinran Chong Aiping Song Zhiyong Guan Weimin Fang Fadi Chen | 2019 | Horticulture Research2019，6，1： | 9 |
| 18 | Cytological and Molecular Characteristics of Pollen Abortion in Lily with Dysplastic Tapetum显示文摘Lily was grown worldwide as a fresh cutting flower because of its colorful petals, but its anther contained a large number of pollen grains that cause serious pollen contamination, however, pollen abortion can effectively reduce the level of pollen pollution. Our analysis aims to use cytological observation to detect the critical stage when pollen abortion occurs and to provide comprehensive gene expression information at the transcriptional level. The result showed that pollen abortion in ‘Little Kiss’ began at the mononuclear stage and the callose that covers the microspores failed to degenerate when young pollens were released from the tetrads. In addition, compared with the normally developed one,the tapetum of ‘Little Kiss’ degraded in advance while the degradation of callose was delayed. Furthermore, 103 differentially expressed genes(DEGs) related to the advance degeneration of tapetum cells and callose were found in the expression levels, including 22 transcription factors(TFs). In particular, two β-glucanase genes(endo-1,3(4)-β-glucanase, exo-β-glucanase) responsible for callose degeneration were significantly down-regulated. These results suggested that pollen abortion may occur at mononuclear stage and that early degeneration of tapetum cells resulted in a significant down-regulation of β-glucanase genes. As a result, the callose to cover microspores impedes the formation of pollen walls, which may possibly lead to pollen abortion. | Xueqian Wang Ze Wu Lanqing Wang Meijiao Wu Dehua Zhang Weimin Fang Fadi Chen Nianjun Teng | 2019 | Horticultural Plant Journal2019，5，6： | 9 |
| 19 | Relationship between the number of tapping-induced secondary laticifer lines and rubber yield among Hevea germplasm显示文摘The lack of suitable early selection parameters means that traditional rubber breeding for yield is time consuming and inefficient. Laticifer is a tissue specific fo natural rubber biosynthesis and storage in rubber tree. The number of the secondary laticifers in the trunk bark tissues is positively correlated with rubber yield in the matured rubber trees that are regularly tapped. In the present study the rubber yield from 280 of 4–5 year-old virgin trees from7 cross combinations was compared with the number o newly differentiated secondary laticifers caused by tapping. Results showed that the number of tapping induced lines of secondary laticifers varied in differen germplasm and was positively related to the rubber yield indicating this could be used as a suitable parameter fo early evaluation of yield potential of rubber trees. | Yueyi CHEN Xinsheng GAO Xiaofei ZHANG Weimin TIAN | 2016 | Frontiers of Agricultural Science and Engineering2016，3，4： | 9 |
| 20 | Efficacy of chalcopyrite bioleaching using a pure and a mixed bacterium显示文摘To determine the efficacy of chalcopyrite bioleaching using pure cultures of Thiobacillus ferrooxidans or Thiobacillus thiooxidans and a mixed culture composed of Thiobacillus ferrooxidans and Thiobacillus thiooxidans, experiments were carried out in shake flasks with [Fe^(2+)] 4 g.L^(-1) and S 1 g.L^(-1) at pH=1.80, 130 r/min and 30℃. The tests showed that the copper extraction in a mixed culture composed of Thiobacillus ferrooxidans and Thiobacillus thiooxidans is higher than that in a pure culture. On the other hand, an important potential of Thiobacillus thiooxidans to leaching chalcopyrite was indicated. Thiobacillus thiooxidans can prevent jarosites accumulating on the substrate and allow further copper to dissolute through the action of ferric ion. The selection of the suitable pH in a leaching solution would be significant. Thiobacillus thiooxidans and Thiobacillus ferrooxidans play an important role in the bioleaching process. Finally, the mechanism and the reason for iron precipitation were also discussed in detail. | Muqing Qiu Shuiying Xiong Weimin Zhang | 2006 | Journal of University of Science and Technology Beijing2006，13，1： | 8 |
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