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| 1 | Newborn screening with targeted sequencing:a multicenter investigation and a pilot clinical study in China显示文摘Different newborn screening(NBS) programs have been practiced in many countries since the 1960 s. It is of considerable interest whether next-generation sequencing is applicable in NBS. We have developed a panel of 465 causative genes for 596 early-onset, relatively high incidence, and potentially actionable severe inherited diseases in our Newborn Screening with Targeted Sequencing(NESTS) program to screen 11,484 babies in 8 Women and Children’s hospitals nationwide in China retrospectively. The positive rate from preliminary screening of NESTS was 7.85%(902/11,484). With 45.89%(414/902) follow-up of preliminary positive cases, the overall clinically confirmative diagnosis rate of monogenic disorders was 12.07%(50/414), estimating an average of 0.95%(7.85% × 12.07%) clinical diagnosis rate, suggesting that monogenic disorders account for a considerable proportion of birth defects. The disease/gene spectrum varied in different regions of China. NESTS was implemented in a hospital by screening 3923 newborns to evaluate its clinical application. The turn-around time of a primary report, including the sequencing period of < 7 days, was within 11 days by our automatic interpretation pipeline. Our results suggest that NESTS is feasible and cost-effective as a first-tier NBS program, which will change the status of current clinical practice of NBS in China. | Chanjuan Hao Ruolan Guo Xuyun Hu Zhan Qi Qi Guo Xuanshi Liu Yuanhu Liu Yanhua Sun Xiaofen Zhang Feng Jin Xiujie Wu Ren Cai Dingyuan Zeng Xijiang Hu Xiaohua Wang Xiaoping Ji Wenjie Li Quansheng Xing Lanfang Mu Xiulian Jiang Xue Yang Weimin Yang Yan Zhang Qianli Yin Xin Ni Wei Li | 2022 | Journal of Genetics and Genomics2022，49，1： | 7 |
| 2 | Characteristics of distribution of Mycobacterium tuberculosis lineages in China显示文摘The genotyping methods of Mycobacterium tuberculosis would dramatically improve our understanding of the molecular epidemiology of tuberculosis. 3,929 isolates, from a National Survey of Drug-Resistant Tuberculosis in 2007 in China, were successfully genotyped by large sequence polymorphisms and 15 loci variable number tandem repeats. We found that 2,905(2,905/3,929, 73.9%) cases belonged to Lineage 2, dominated in the east and central regions, 975 cases(975/3,929, 24.8%) were Lineage 4, highly prevailed in the west regions, and 36 and 13 cases were Lineage 3 and Lineage 1, respectively. We also explored the associations between lineages(Lineage 2 vs. Lineage 4) and clinical characteristics by logistic regression. For Lineage 2, the risk factors were Han-ethnicity population and fever. However, for Lineage 4, they were occupation(farmer), and degree of education(non-literate). Fully understanding of the distribution of Mycobacterium tuberculosis lineage and its risk factors would play a critical role in tuberculosis prevention, control, and treatment. | Haixia Chen Li He Chao Cai Jingyi Liu Junnan Jia Liang Ma Hairong Huang Lixia Wang Xumin Ni Jimin Gao Weimin Li | 2018 | Science China(Life Sciences)2018，61，6： | 7 |
| 3 | Proteomics provides individualized options of precision medicine for patients with gastric cancer显示文摘While precision medicine driven by genome sequencing has revolutionized cancer care,such as lung cancer,its impact on gastric cancer(GC)has been minimal.GC patients are routinely treated with chemotherapy,but only a fraction of them receive the clinical benefit.There is an urgent need to develop biomarkers or algorithms to select chemo-sensitive patients or apply targeted therapy.Here,we carried out retrospective analyses of 1,020 formalin-fixed,paraffin-embedded GC surgical resection samples from 5 hospitals and developed a mass spectrometry-based workflow for proteomic subtyping of GC.We identified two proteomic subtypes:the chemo-sensitive group(CSG)and the chemo-insensitive group(CIG)in the discovery set.The 5-year overall survival of CSG was significantly improved in patients who had received adjuvant chemotherapy after surgery compared with those who received surgery only(64.2%vs.49.6%;Cox P-value=0.002),whereas no such improvement was observed in CIG(50.0%vs.58.6%;Cox P-value=0.495).We validated these results in an independent validation set.Further,differential proteome analysis uncovered 9 FDA-approved drugs that may be applicable for targeted therapy of GC.A prospective study is warranted to test these findings for future GC patient care. | Wenwen Huang Dongdong Zhan Yazhuo Li Nairen Zheng Xin Wei Bin Bai Kecheng Zhang Mingwei Liu Xuefei Zhao Xiaotian Ni Xia Xia Jinwen Shi Cheng Zhang Zhihao Lu Jiafu Ji Juan Wang Shiqi Wang Gang Ji Jipeng Li Yongzhan Nie Wenquan Liang Xiaosong Wu Jianxin Cui Yongsheng Meng Feilin Cao Tieliu Shi Weimin Zhu Yi Wang Lin Chen Qingchuan Zhao Hongwei Wang Lin Shen Jun Qin | 2021 | Science China(Life Sciences)2021，64，8： | 3 |
| 4 | Integrated single-cell RNA sequencing analysis reveals distinct cellular and transcriptional modules associated with survival in lung cancer显示文摘Lung adenocarcinoma(LUAD)and squamous carcinoma(LUSC)are two major subtypes of non-small cell lung cancer with distinct pathologic features and treatment paradigms.The heterogeneity can be attributed to genetic,transcriptional,and epigenetic parameters.Here,we established a multi-omics atlas,integrating 52 single-cell RNA sequencing and 2342 public bulk RNA sequencing.We investigated their differences in genetic amplification,cellular compositions,and expression modules.We revealed that LUAD and LUSC contained amplifications occurring selectively in subclusters of AT2 and basal cells,and had distinct cellular composition modules associated with poor survival of lung cancer.Malignant and stage-specific gene analyses further uncovered critical transcription factors and genes in tumor progression.Moreover,we identified subclusters with proliferating and differentiating properties in AT2 and basal cells.Overexpression assays of ten genes,including sub-cluster markers AQP5 and KPNA2,further indicated their functional roles,providing potential targets for early diagnosis and treatment in lung cancer. | Li Zhang Yiming Zhang Chengdi Wang Ying Yang Yinyun Ni Zhoufeng Wang Tingting Song Menglin Yao Zhiqiang Liu Ningning Chao Yongfeng Yang Jun Shao Zhidan Li Ran Zhou Li Chen Dan Zhang Yuancun Zhao Wei Liu Yupeng Li Ping He Jing-wen Lin Yuan Wang Kang Zhang Lu Chen Weimin Li | 2022 | Signal Transduction and Targeted Therapy2022，7，2： | 2 |
| 5 | Optimization models and a GA-based algorithm for stochastic time-cost trade-off problem 显示文摘 | Ke Hua Ma Weimin Ni Yaodong | 2009 | Applied Mathematics and Computation2009，215，1： | 1 |
| 6 | Application of chitosan as flocculant for coprecipitation of Mn(Ⅱ) and suspended solids from dual-alkali FGD regenerating process显示文摘 | Wu Zhongbiao Ni Weimin Guan Baohong | 2008 | Journal of Hazardous Materials2008，152，2： | 1 |
| 7 | Removal of Mn(Ⅱ) and Zn(Ⅱ) ions from flue gas desulfurization wastewater with wa-ter-soluble chitosan显示文摘 | Guan Baohong Ni Weimin Wu Zhongbiao | 2009 | Separation and Purification Technology2009，65，3： | 1 |
| 8 | Visibility, air quality and daily mortality in Shanghai, China显示文摘 | Wei Huang Jianguo Tan Haidong Kan Ni Zhao Weimin Song Guixiang Song Guohai Chen Lili Jiang Cheng Jiang Renjie Chen Bingheng Chen | 2009 | Science of the Total Environment2009，，10： | 1 |
| 9 | SPAG9 is overexpressed in human astrocytoma and promotes cell proliferation and invasion显示文摘 | Fuxin Yi Weimin Ni Wenda Liu Xiaodong Pan Xiubin Han Lei Yang Xiangquan Kong Rui Ma Rui Chang | 2013 | Tumor Biology2013，，5： | 1 |
| 10 | Application of chitosan as flocculant for coprecipitation of Mn ( Ⅱ ) and suspended solids from dual-alkali FGD regenerating process 显示文摘 | WU Zhongbiao NI Weimin GUAN Baohong | 2008 | Journ of Hazardous Materials2008，152，2： | 1 |
| 11 | Application of chitosanas flocculant for coprecipitation of Mn ( II )and suspendedsolids from dual-alkali FGD regenerating process 显示文摘 | Wu Zhongbiao' Ni Weimin Guan Baohong | 2008 | Journal of Hazardous Materials2008，152，2： | 1 |
| 12 | Expression and biological role of CIP2A in human astrocytoma显示文摘 | Fuxin Yi Weimin Ni Weixian Liu Jinyang Bai Wenbin Li | 2013 | Molecular Medicine Reports2013，，5： | 1 |
| 13 | Performance analysis of MVDR Algorithm in the Presence of Amplitude and Phase Errors显示文摘 | SU Weimin GU Hong NI Jinlin LIU Guosui | 2001 | IEEE Trans on Antennas and Propagation2001，49，12： | 1 |
| 14 | The heterogeneous immune landscape between lung adenocarcinoma and squamous carcinoma revealed by single-cell RNA sequencing显示文摘A thorough interrogation of the immune landscape is crucial for immunotherapy strategy selection and prediction of clinical responses in non-small-cell lung cancer(NSCLC)patients.Single-cell RNA sequencing(scRNA-seq)techniques have prompted the opportunity to dissect the distinct immune signatures between lung adenocarcinoma(LUAD)and lung squamous cell carcinoma(LUSC),the two major subtypes of NSCLC.Here,we performed scRNA-seq on 72,475 immune cells from 40 samples of tumor and matched adjacent normal tissues spanning 19 NSCLC patients,and drew a systematic immune cell transcriptome atlas.Joint analyses of the distinct cellular compositions,differentilly expressed genes(DEGs),cell-cell interactions,pseudotime trajectory,transcriptomic factors and prognostic factors based on The Cancer Genome Atlas(TCGA),revealed the central roles of cytotoxic and effector T and NK cells and the distinct functional macrophages(Mo)subtypes in the immune microenvironment heterogeneity between LUAD and LUSC.The dominant subtype of Mo was FABP4-Mp in LUAD and SPP1-Mo in LUSC.Importantly,we identifieda novel lymphocyte-related Mo cluster,which we named SELENOP-Mo,and further established its antitumor role in both types,especially in LUAD.Our comprehensive depiction of the immune heterogeneity and definition of Mp clusters could help design personalized treatment for lung cancer patients in clinical practice. | Chengdi Wang Qiuxiao Yu Tingting Song Zhoufeng Wang Lujia Song Ying Yang Jun Shao Jingwei Li Yinyun Ni Ningning Chao Li Zhang Weimin Li | 2022 | Signal Transduction and Targeted Therapy2022，7，9： | 1 |
| 15 | Application of chitosan as flocculant for coprecipitation of Mn ( II ) and suspended solids from dual-alkali FGD regenerating process显示文摘 | Wu Zhongbiao Ni Weimin Guan Baohong | 2008 | Journal of Hazardous Materials2008，152，2： | 1 |
| 16 | E3泛素连接酶SCF^(EBF1/2)通过降解PIF3促进植物的光形态建成显示文摘文章简介光提供了植物生长所需要的能量,同时作为核心环境信号因子调控着植物各个阶段的生长发育。此前,通过筛选与光受体相互作用的因子,人们鉴定到光信号通路的核心转录因子PIF3。 | 董杰 Weimin Ni Renbo Yu 邓兴旺 陈浩东 魏宁 | 2018 | 科学新闻2018，0，4： | 0 |
| 17 | The top cited articles on glioma stem cells in Web of Science显示文摘BACKGROUND: Glioma is the most common intracranial tumor and has a poor patient prognosis. The presence of brain tumor stem cells was gradually being understood and recognized, which might be beneficial for the treatment of glioma. OBJECTIVE: To use bibliometric indexes to track study focuses on glioma stem cell, and to investigate the relationships among geographic origin, impact factors, and highly cited articles indexed in Web of Science. METHODS: A list of citation classics for glioma stem cells was generated by searching the database of Web of Science-Expanded using the terms 'glioma stem cell' or 'glioma, stem cell'' or 'brain tumor stem cell'. The top 63 cited research articles which were cited more than 100 times were retrieved by reading the abstract or full text if needed. Each eligible article was reviewed for basic information on subject categories, country of origin, journals, authors, and source of journals. Inclusive criteria: (1) articles in the field of glioma stem cells which was cited more than 100 times; (2) fundamental research on humans or animals, clinical trials and case reports; (3) research article; (4) year of publication: 1899-2012; and (5) citation database: Science Citation Index-Expanded. Exclusive criteria: (1) articles needing to be manually searched or accessed only by telephone; (2) unpublished articles; and (3) reviews, conference proceedings, as well as corrected papers. RESULTS: Of 2 040 articles published, the 63 top-cited articles were published between 1992 and 2010. The number of citations ranged from 100 to 1 754, with a mean of 280 citations per article. These citation classics came from nineteen countries, of which 46 articles came from the United States. Duke University and University of California, San Francisco led the list of classics with seven papers each. The 63 top-cited articles were published in 28 journals, predominantly Cancer Research and Cancer Cell, followed by Cell Stem Cell and Nature. CONCLUSION: Our bibliometric analysis provides a historical perspective on the progress of glioma stem cell research. Articles originating from outstanding institutions of the United States and published in high-impact journals are most likely to be cited. | Fuxin Yi Jun Ma Weimin Ni Rui Chang Wenda Liu Xiubin Han Dongxiao Pan Xingbo Liu Jianwu Qiu | 2013 | Neural Regeneration Research2013，8，15： | 0 |
| 18 | A strategy based on water soluble coal tar pitches to construct MnO_(2)@C composite materials with high electrochemical performance显示文摘The water soluble coal tar pitches(WS-CTPs)were successfully prepared and used to construct the MnO_(2)@C composite materials by a hydrothermal method.It is interestingly observed that the structures and morphologies of MnO_(2)@C materials can be controlled by controlling the dosages of WS-CTPs and KMnO4.Meanwhile,it is aware that MnO_(2)exists in the MnO_(2)@C materials in an amorphous state.Compared with MnO_(2),MnO_(2)@C materials output a remarkable improvement in electrochemical performance.For instance,MnO_(2)@C-0.3 shows the storage capacity at 965.7 mA h g^(−1)after 300 cycles at a current density of 0.1 A g^(−1).In addition,after 600 cycles at a current density of 1.0 A g^(−1),the storage capacity of MnO_(2)@C-0.3 still keeps 450.3 mA h g^(−1),indicating that MnO_(2)@C-0.3 owns tremendous cycle stability at a high current density.In view of the fact that the coal tar pitches possess great cost advantages,the strategy of using WS-CTPs as a carbon source to cover the metal oxides is a competitive way to expand the application of metal oxides in the fabrication of electrodes of LIBs. | LIU Bo WANG Kun GUO ZhaoQi HAN BeiBei XU GuiYing JU DongYing AN BaiGang LI YongCheng NI ZhenYong ZHOU WeiMin | 2024 | Science China(Technological Sciences)2024，67，1： | 0 |
| 19 | Proteomic identification of potential target proteins regulated by an ASK1-mediated proteolysis pathway显示文摘ASK1 (ARABIDOPSIS 象 SKP1 一样) 蛋白质是招募的 ubiquitin ligase 建筑群指向的 SCF (Skp1-Cullin-F 盒子蛋白质) 的一个批评部件为由 26S proteosome 的降级的蛋白质。为了调查蛋白质,那被调停 ASK1 的解朊作用小径在 Arabidopsis 花影响,我们用二维的电气泳动(2-DE ) 比较了 Arabidopsis 野类型和 ask1 异种花芽的 proteomes。有在与野类型花相比的 ask1 异种花的更高或更低的丰富的十个蛋白质点被切除并且使分析遭到了到进一步集体的 spectrometry (MS ) 。结果证明他们是涉及相片形态发生,生理节奏的摆动,翻译以后的过程,压力反应和房间扩大或延伸的蛋白质,建议那些过程在 ask1 异种被影响。基因也是的这些的抄本层次基于 Affymetrix 基因芯片比较了微数组数据。没有重要差别为大多数基因被观察,建议有在 ask1 异种的累积的提高的层次的蛋白质能是一条调停 ASK1 的解朊作用小径调整的候选人目标。阐明的这些结果帮助在 Arabidopsis 发展过程并且也的 ASK1 的多种的功能表明关于基因功能学习蛋白质层次的重要性和必要性。 | Xiaofeng Wang Weimin Ni Xiaochun Ge Jiejin Zhang Hong Ma Kaiming Cao | 2006 | Cell Research2006，16，5： | 0 |
| 20 | Efficacy of early prone or lateral positioning in patients with severe COVID-19:a single-center prospective cohort显示文摘Background:Position intervention has been shown to improve oxygenation,but its role in non-invasively ventilated patients with severe COVID-19 has not been assessed.The objective of this study was to investigate the efficacy of early position intervention on non-invasively ventilated patients with severe COVID-19.Methods:This was a single-center,prospective observational study in consecutive patients with severe COVID-19 managed in a provisional ICU at Renmin Hospital of Wuhan University from 31 January to 15 February 2020.Patients with chest CT showing exudation or consolidation in bilateral peripheral and posterior parts of the lungs were included.Early position intervention(prone or lateral)was commenced for>4 hours daily for 10 days in these patients,while others received standard care.Results:The baseline parameters were comparable between the position intervention group(n=17)and the standard care group(n=35).Position intervention was well-tolerated and increased cumulative adjusted mean difference of SpO_(2)/FiO_(2)(409,95%CI 86 to 733)and ROX index(26,95%CI 9 to 43)with decreased Borg scale(−9,95%CI−15 to−3)during the first 7 days.It also facilitated absorption of lung lesions and reduced the proportion of patients with high National Early Warning Score 2(≥7)on days 7 and 14,with a trend toward faster clinical improvement.Virus shedding and length of hospital stay were comparable between the two groups.Conclusions:This study provides the first evidence for improved oxygenation and lung lesion absorption using early position intervention in non-invasively ventilated patients with severe COVID-19,and warrants further randomized trials. | Zhong Ni Kaige Wang Ting Wang Yuenan Ni Wei Huang Ping Zhu Tao Fan Ye Wang Bo Wang Jun Deng Zhicheng Qian Jiasheng Liu Wenhao Cai Shanling Xu Yu Du Gang Wang Zongan Liang Weimin Li Jianfei Luo Fengming Luo Dan Liu | 2020 | Precision Clinical Medicine2020，3，4： | 0 |
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