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| 1 | Comparative expression analysis of three genes from the Arabidopsis vacuolar Na^+/H^+ antiporter (AtNHX) family in relation to abiotic stresses显示文摘Na^+/H^+ antiporters (NHX) are ubiquitous transmembrane proteins that play a key role in salt tolerance of plants. In this study, the sequence of 3 Arabidopsis NHX gene (AtNHX2―4) were compared with other AtNHX members. Putative cis-elements analysis identified elements that have been associated with stress responses. The activities of the promoters AtNHX2―4 were studied in transgenic plants carrying corresponding promoter-β-glucuronidase (GUS) fusions. The AtNHX2 promoter-GUS analysis indicated that AtNHX2 was expressed in constitutive pattern with high GUS activity in roots and leaves. AtNHX2 promoter activity was not up-regulated by NaCl or abscisic acid (ABA), in contrast to the AtNHX1 promoter which was previously studied. The AtNHX3 and AtNHX4 promoters showed tissue-specific activities. Strong GUS activity was detected in roots and vascular bundles of the stele in plants carry-ing an AtNHX4 promoter-GUS fusion, and GUS activity increased under salt stress suggesting a func-tion related to salt tolerance. Transgenic plants carrying the AtNHX3 promoter-GUS fusion showed strong GUS activity in petals, stamens and tops of siliques, suggesting a possible role of AtNHX3 in flower and seed development. Results of histochemical analysis suggested that AtNHX2―4 are involved in divergent functions and are differentially regulated under abiotic stress. The structure of AtNHX4 was predicted to include 12 transmembrane regions and a NHX domain. Overexpression of AtNHX4 in Arabidopsis transgenic lines confers greater salt tolerance than in wild type plants. These results suggest that AtNHX4 may encode a putative vacuolar NHX that plays an important role in salt tolerance. | WANG WeiQuan LI Yin ZHANG YiYue YANG CuiPing ZHENG NuoYan XIE Qi | 2007 | Chinese Science Bulletin2007，52，13： | 5 |
| 2 | Antagonism between coral pathogen Vibrio coralliilyticus and other bacteria in the gastric cavity of scleractinian coral Galaxea fascicularis显示文摘Scleractinian corals host numerous microbial symbionts with different types of interactions. The gastric cavity of scleractinian coral, as a semiclosed subenvironment with distinct chemical characteristics(e.g., dissolved O2, pH, alkalinity, and nutrients), harbors a distinct microbial community and a diverse array of bacteria that can be pathogenic or beneficial. Galaxea fascicularis is one of the dominant massive scleractinian coral species on inshore fringing reefs in the northern South China Sea.Although the abundance of coral-associated bacteria has been investigated in G. fascicularis, less is known about the microorganisms in the gastric cavity. In this study, we specially isolated cultivable bacterial strains from the gastric cavity of G.fascicularis collected from Hainan Island using a noninvasive sampling approach. Among the 101 representative bacterial strains, one Vibrio coralliilyticus strain, SCSIO 43001, was found to be a temperature-dependent opportunistic pathogen of G.fascicularis. The antagonistic activity between the 100 strains and V. coralliilyticus SCSIO 43001 was tested using a modified Burkholder diffusion assay. Our results showed that V. coralliilyticus SCSIO 43001 inhibits the growth of Erythrobacter flavus and Sphingomonas yabuuchiae. Additionally, we found that three Pseudoalteromonas strains showed moderate to high antibacterial activity against V. coralliilyticus SCSIO 43001 and several other coral-associated Gram-negative bacterial strains.These results suggest that competition between the coral pathogen and other bacteria also occurs in the gastric cavity of coral, and Pseudoalteromonas strains in the gastric cavity of G. fascicularis may provide a protective role in the defense against coinhabiting coral pathogens at elevated temperature. | Kaihao TANG Waner ZHAN Yiqing ZHOU Tao XU Xiaoqing CHEN Weiquan WANG Zhenshun ZENG Yan WANG Xiaoxue WANG | 2020 | Science China Earth Sciences2020，63，1： | 2 |
| 3 | Apelin inhibits motor neuron apoptosis in the anterior horn following acute spinal cord and sciatic nerve injuries显示文摘Rat models of acute spinal cord injury and sciatic nerve injury were established.Apelin expression in spinal cord tissue was determined.In normal rat spinal cords,apelin expression was visible;however,2 hours post spinal cord injury,apelin expression peaked.Apelin expression increased 1 day post ligation of the sciatic nerve compared with normal rat spinal cords,and peaked at 3 days.Apelin expression was greater in the posterior horn compared with the anterior horn at each time point when compared with the normal group.The onset of neuronal apoptosis was significantly delayed following injection of apelin protein at the stump of the sciatic nerve,and the number of apoptotic cells after injury was reduced when compared with normal spinal cords.Our results indicate that apelin is expressed in the normal spinal cord and central nervous system after peripheral nerve injury.Apelin protein can reduce motor neuron apoptosis in the spinal cord anterior horn and delay the onset of apoptosis. | Zhiyue Li Weiguo Wang Qun Zhao Weiquan Ning Bin Yang Suling Zhang Siyin Feng | 2011 | Neural Regeneration Research2011，6，20： | 2 |
| 4 | Flexible Humidity Sensitive Fiber with Swellable Metal-Organic Frameworks显示文摘Humidity sensors have become an essential need for improving daily life quality.Here,a novel humidity sensitive fiber was fabricated using the soft polydimethylsiloxane(PDMS)as the core layer and the non-swellable polyvinylidenedifluoride(PVDF)embedded with a swellable MIL-88A as the outer layer.Noticeably,the PDMS fiber was chosen as a carrier due to its smoothness,softness and good tensile properties.The isoreticular FeIII dicarboxylate MIL-88 family with swellable metal-organic frameworks(MOFs)can undergo reversible dynamic structural transformations with a response to external humidity change.The resulted fiber with swellable MOFs showed three kinds of deformation for a single solvent and also had good deformation performance for two-component miscible solution with different volume ratios.As a proof of concept,a shape-memory effect at relative humidity from 10 to 90%and the simulated salt solutions instead of relative humidity changes were used to evaluate humidity change.In addition,a deep insight into the self-shape-change mechanism among those phenomena was investigated,wherein expansion deformation of the PDMS fiber as well as the structural transforma-tion of the MIL-88A worked in different conditions. | Sijia Lv Luyizheng Shuai Wenfeng Ding Weiquan Ke Bing Wang Junmin Wan | 2021 | Advanced Fiber Materials2021，3，2： | 2 |
| 5 | A large insert Thellungiella halophila BIBAC library for genomics and identification of stress tolerance genes显示文摘 | Weiquan Wang Yaorong Wu Yin Li Jiaying Xie Zhonghui Zhang Zhiyong Deng Yiyue Zhang Cuiping Yang Jianbin Lai Huawei Zhang Hongyan Bao Sanyuan Tang Chengwei Yang Peng Gao Guixian Xia Huishan Guo Qi Xie | 2010 | Plant Molecular Biology (-)2010，，1： | 1 |
| 6 | Immunohistochemical and in situ hybridization studies of gonadotropin releasing hormone (GnRH) and its receptor in rat digestive tract显示文摘 | Huang WeiQuan Yao Bing Sun Lan Pu RuoLei Wang Lei Zhang RongQing | 2001 | Life Sciences2001，，15： | 1 |
| 7 | Recommendation agents for electronic commerce: Effects of explanation facilities on trusting beliefs显示文摘 | Wang Weiquan and Benbasat I | 2007 | Journal of Management Information Systems2007，23，4： | 1 |
| 8 | Multimedia-based interactive advising technology for online consumer decision support显示文摘 | Zhenhui Jiang Weiquan Wang Izak Benbasat | 2005 | Communications of the ACM2005，，9： | 1 |
| 9 | Cr (VI) Removal from Aqueous Solutions by Hydrothermal Synthetic Layered Double Hydroxides: Adsorption Performance, Coexisting Anions and Regeneration Studies显示文摘 | Wenwen Wang Jiabin Zhou Gopal Achari Jiaguo Yu Weiquan Cai | 2014 | Colloids and Surfaces A: Physicochemical and Engineering Aspects2014，，： | 1 |
| 10 | Laser Cooling and Deceleration of Neutral Atoms by Red-Shifted Diffuse Light显示文摘We report a cooling and deceleration experiment of a thermal beam by using a nearly resonant red-shifted diffuse light in an optical integral sphere cavity.By using of this red-shifted diffuse light,the velocity of a part of thermal sodium atoms is cooled to 380m/s and the velocity width of cooled atoms is about 18m/s.The mechanism of this kind of laser cooling and the experimental results are discussed. | CHEN Hongxin CAI Weiquan LIU Liang SHU Wei LI Fosheng WANG Yuzhu | 1994 | Chinese Physics Letters1994，11，9： | 1 |
| 11 | Reliability analysis for vertical integration of protection,measurement,merge unit,and intelligent terminal device显示文摘The reliability analysis of vertically integrated protection devices is crucial for designing International Electrotechnical Commission(IEC)61850-based substations.This paper presents the hardware architecture of a four-inone vertically integrated device and the information transmission path of each function based on the functional information transmission chain of protection devices,measurement and control devices,merging units,and intelligent terminals.Additionally,a reliability analysis model of the protection device and its protection system is constructed using the fault tree analysis method while considering the characteristics of each module of the vertically integrated device.The stability probability of the protection system in each state is analyzed by combining the state-transfer equations of line and busbar protection with a Markov chain.Finally,the failure rate and availability of the protection device and its protection system are calculated under different ambient temperatures using a 110 kV intelligent substation as an example.The sensitivity of each device module is analyzed. | Ying Li Weiquan Wang Liang Zhang Zhujian Liang Zhenli Xu Yuansheng Liang | 2023 | Global Energy Interconnection2023，6，6： | 1 |
| 12 | FOXP3 Is an X-Linked Breast Cancer Suppressor Gene and an Important Repressor of the HER-2/ErbB2 Oncogene显示文摘 | Tao Zuo Lizhong Wang Carl Morrison Xing Chang Huiming Zhang Weiquan Li Yan Liu Yin Wang Xingluo Liu Michael W.Y. Chan Jin-Qing Liu Richard Love Chang-gong Liu Virginia Godfrey Rulong Shen Tim H.-M. Huang Tianyu Yang Bae Keun Park Cun-Yu Wang Pan Zheng Yan | 2007 | Cell2007，，7： | 1 |
| 13 | Preparation and Regeneration Conditions of Ceratocystis paradoxa Protoplast显示文摘[Objective]The paper was to reveal the preparation and regeneration conditions of Ceratocystis caradoxa protoplast.[Method]Using multi-factors orthogonal test,the effects of hypha age,enzyme system,time of hydrolysis,hydrolysis temperature,osmotic pressure stabilizer and regeneration medium on C.paradoxa protoplast were studied.[Result]The optimum condition for preparing protoplast were conidia cultured in liguid SYM medium for 24 h,enzyme mixture of 1%driselase and 1%lytic enzyme used to digest hypha for 1.5 h,0.7 mol/L MgSO4·7H2O used as osmotic stabilizer,or individual enzyme 1%driselase used to digest hypha for 1.5 h,1.0 mol/L mannitol as osmotic stabilizer.The regeneration rate was over 40%under C.paradoxa protoplast regenerating on CM medium with mannitol and hydrolysis for 2 h.[Conclusion]Higher protoplast yield and higher regeneration rate could be obtained under above conditions,which is beneficial for transformation and further research. | Niu Xiaoqing Yu Fengyu Wang Yenan Tang Qinghua Zhu Hui Qin Weiquan | 2019 | Plant Diseases and Pests2019，10，5： | 1 |
| 14 | FOXP3 Is an X-Linked Breast Cancer Suppressor Gene and an Important Repressor of the HER-2/ErbB2 Oncogene显示文摘 | Tao Zuo Lizhong Wang Carl Morrison Xing Chang Huiming Zhang Weiquan Li Yan Liu Yin Wang Xingluo Liu Michael W.Y. Chan Jin-Qing Liu Richard Love Chang-gong Liu Virginia Godfrey Rulong Shen Tim H.-M. Huang Tianyu Yang Bae Keun Park Cun-Yu Wang Pan Zheng Yan | 2007 | Cell2007，，7： | 1 |
| 15 | Interactive decision aids for consumer decision making in e-commerce: The influence of perceived strategy restrictiveness 显示文摘 | Wang Weiquan Benbasat I | 2009 | MIS Quarterly2009，33，2： | 1 |
| 16 | Recommendation Agents for Electronic Commerce: Effects of Explanation Facilities on Trusting Beliefs显示文摘 | Weiquan Wang and Izak Benbasat | 2007 | Journal of Management Information Systems2007，，4： | 1 |
| 17 | Detection of CD2 expression in chicken hematogenic embryo yolk sac lymphoid cells prior to thymus genesis显示文摘从在舞台 16 点的小鸡胚胎的淋巴的单音的原子房间在胎儿的肝和胸腺开始以前被收集学习造血的蛋黄囊的区别和功能并且检测 CD2 是否发生在淋巴的单音的原子房间的表面上。显型和房间表面蛋白质 E 受体的功能的活动和胚胎的 E + 房间的超微结构与成熟 T 房间的那些相比。我们的结果显示在胚胎的淋巴细胞和成熟 T 房间的 E 受体之间的 99.36% 相同。另外的类似包括分子的分发,动机,形成一个红血球插座,在胚胎的淋巴细胞之间被检测并且成熟的能力,受体 ligand 建筑群的结构,和信号隧道的符合构造表示 CD2 T 房间。这些结果显示 CD2 已经在胎儿的肝和胸腺开始以前被表示并且它的表示不依赖于 thymic 微型环境。 | Dongyu Zhou Jigui Wang Weiquan Liu Rongxiu Liu Yuehu Pei | 2008 | Progress in Natural Science:Materials International2008，18，12： | 1 |
| 18 | Velocity Selection of Atomic Beam by Laser显示文摘It is possible to deflect a group of atoms with a certain velocity from an effusive atomic beam if we use a properly detuned laser beam with a small angle off orthogonal to the atomic beam.Theoretical and initial experimental results of this simple method for velocity selection are given. | CAI Weiquan CHEN Hongxin LI Fusheng SHU Wei SUN Shundi WANG Yuzhu | 1995 | Chinese Physics Letters1995，12，5： | 0 |
| 19 | Identification of bacteria-derived urease in the coral gastric cavity显示文摘Nitrogen cycle is critical to maintain a healthy coral reef ecosystem.Urea can provide a source of nitrogen for coral holobiont and is important for coral calcification through degradation by urease.Despite the involvement of coral-associated bacteria in nitrogen fixation,nitrification and denitrification are well recognized,to what extend urea degradation by bacteria contributing to the urea utilization by coral holobiont remains to be investigated.In this study,we demonstrate that the urea utilization is a common feature of Halomonas spp.that is a dominant genus in cultivable coral-associated bacteria.A urease operon was characterized by genome sequencing and gene knock-out technique in Halomonas meridiana SCSIO 43005,isolated from the gastric cavity of healthy scleractinian coral Galaxea fascicularis.H.meridiana showed high urease activity which was induced by urea and deletion of the urease operon reduced the capability to use urea as solo nitrogen source.Furthermore,approximately 1/3 coral-associated bacteria in the IMG/M database possess complete urease operons indicating the involvement of bacteria-derived ureases in coral holobiont.These results suggest that urease from coral-associated bacteria might be important player in the nitrogen cycling of coral reefs. | Yiqing ZHOU Kaihao TANG Pengxia WANG Weiquan WANG Yan WANG Xiaoxue WANG | 2020 | Science China Earth Sciences2020，63，10： | 0 |
| 20 | Transcritption regulation of soybean ribulose-1,5-bisphos-phate carboxylase small sub-unit gene by external factors显示文摘Ribulose-l,5-bisphosphate carboxylase small subunit gene (rbcS) is present with multi-gene family in plant genome. In Glycine max, the rbcS polypeptide (EC4.1.1.39) is encoded by a gene family containing 4-8 members. Three full-length rbcS cDNA clones were isolated and characterized from soybean seedlings, and both of their nucleotide and amino acid sequences showed high similarity. Differential accumulation of the rbcS mRNA was observed among roots, hypocotyls, cotyledons, epicotyls and leaves. The rbcS genes were up-regulated by various external factors such as salicylic acid (SA), salt stress and drought stress. The expression level of rbcS genes after being treated by 2.0 mmol/L SA and0.4% NaCl, respectively, is 2.5-3.0-fold as high as that of control sample. Moreover, soybean rbcS mRNA was accu-mulated with diurnal variation but easily influenced by light and low temperature. | HE Chaoying, WANG Weiquan,DONGFANG Yang, ZHANG Jinsong, GAI Junyi& CHEN ShouyiPlant Biotechnology Laboratory, Institute of Genetics, Chinese Academy of Sciences, Beijing 100101, China National Center for Soybean Improvement, Soybean Research Inst | 2002 | Chinese Science Bulletin2002，47，1： | 0 |
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