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| 1 | A Novel Coronavirus Genome Identified in a Cluster of Pneumonia Cases--Wuhan,China 2019−2020显示文摘Emerging and re-emerging pathogens are great challenges to the public health(1).A cluster of pneumonia cases with an unknown cause occurred in Wuhan starting on December 21,2019.As of January 20,2020,a total of 201 cases of pneumonia in China have been confirmed.A team of professionals from the National Health Commission and China CDC conducted epidemiological and etiological investigations.On January 3,2020,the first complete genome of the novelβgenus coronaviruses(2019-nCoVs)was identified in samples of bronchoalveolar lavage fluid(BALF)from a patient from Wuhan by scientists of the National Institute of Viral Disease Control and Prevention(IVDC)through a combination of Sanger sequencing,Illumina sequencing,and nanopore sequencing.Three distinct strains have been identified,the virus has been designated as 2019-nCoV,and the disease has been subsequently named novel coronavirus-infected pneumonia(NCIP). | Wenjie Tan Xiang Zhao Xuejun Ma Wenling Wang Peihua Niu Wenbo Xu George F.Gao Guizhen Wu | 2020 | China CDC weekly2020，2，4： | 95 |
| 2 | The First Imported Case of Monkeypox in the Mainland of China—Chongqing Municipality,China,September 16,2022显示文摘Monkeypox is a zoonotic viral disease caused by the monkeypox virus(MPXV),and historically,all outbreaks have been linked to Africa;however,monkeypox has been posing an alarming challenge to the world in 2022(1)as approximately 60,000 cases have been reported in more than 100 nations and regions worldwide(2).Currently,many cases of monkeypox were identified in many nonendemic countries outside of Central and West Africa,and human-to-human transmission has occurred frequently,especially among men who have sex with men(MSM)presenting new clinical symptoms similar to syphilis and other sexually transmitted infections(3). | Hua Zhao Wenling Wang Li Zhao Sheng Ye Jingdong Song Roujian Lu Hua Zong Changcheng Wu Wei Huang Baoying Huang Yao Deng Ruhan A Wujuan Xie Li Qi Wenbo Xu Hua Ling Wenjie Tan | 2022 | China CDC weekly2022，4，38： | 35 |
| 3 | Long non-coding RNAs in cancer显示文摘Long non-coding RNAs (lncRNAs) are a group of RNA transcripts that exceed 200 nt in length, yet lack significant open reading frames (ORFs) [1–4]. In contrast | GONG ZhaoJian ZHANG ShanShan ZHANG WenLing HUANG HongBin LI Qiao DENG Hao MA Jian ZHOU Ming XIANG Juan Juan WU MingHua LI XiaYu XIONG Wei LI XiaoLing LI Yong ZENG ZhaoYang LI GuiYuan | 2012 | Science China(Life Sciences)2012，55，12： | 22 |
| 4 | Age-related rhesus macaque models of COVID-19显示文摘Background:Since December 2019,an outbreak of the Corona Virus disease 2019(COVID-19)caused by severe acute respiratory syndrome coronavirus(SARS-CoV-2)in Wuhan,China,has become a public health emergency of international concern.The high fatality of aged cases caused by SARS-CoV-2 was a need to explore the possible age-related phenomena with non-human primate models.Methods:Three 3-5 years old and two 15 years old rhesus macaques were intratracheally infected with SARS-CoV-2,and then analyzed by clinical signs,viral replication,chest X-ray,histopathological changes and immune response.Results:Viral replication of nasopharyngeal swabs,anal swabs and lung in old monkeys was more active than that in young monkeys for 14 days after SARS-CoV-2 challenge.Monkeys developed typical interstitial pneumonia characterized by thickened alveolar septum accompanied with inflammation and edema,notably,old monkeys exhibited diffuse severe interstitial pneumonia.Viral antigens were detected mainly in alveolar epithelial cells and macrophages.Conclusion:SARS-CoV-2 caused more severe interstitial pneumonia in old monkeys than that in young monkeys.Rhesus macaque models infected with SARS-CoV-2 provided insight into the pathogenic mechanism and facilitated the development of vaccines and therapeutics against SARS-CoV-2 infection. | Pin Yu Feifei Qi Yanfeng Xu Fengdi Li Peipei Liu Jiayi Liu Linlin Bao Wei Deng Hong Gao Zhiguang Xiang Chong Xiao Qi Lv Shuran Gong Jiangning Liu Zhiqi Song Yajin Qu Jing Xue Qiang Wei Mingya Liu Guanpeng Wang Shunyi Wang Haisheng Yu Xing Liu Baoying Huang Wenling Wang Li Zhao Huijuan Wang Fei Ye Weimin Zhou Wei Zhen Jun Han Guizhen Wu Qi Jin Jianwei Wang Wenjie Tan Chuan Qin | 2020 | Animal Models and Experimental Medicine2020，3，1： | 22 |
| 5 | The mechanisms of sorafenib resistance in hepatocellular carcinoma:theoretical basis and therapeutic aspects显示文摘Sorafenib is a multikinase inhibitor capable of facilitating apoptosis,mitigating angiogenesis and suppressing tumor cell proliferation.In late-stage hepatocellular carcinoma(HCC),sorafenib is currently an effective first-line therapy.Unfortunately,the development of drug resistance to sorafenib is becoming increasingly common.This study aims to identify factors contributing to resistance and ways to mitigate resistance.Recent studies have shown that epigenetics,transport processes,regulated cell death,and the tumor microenvironment are involved in the development of sorafenib resistance in HCC and subsequent HCC progression.This study summarizes discoveries achieved recently in terms of the principles of sorafenib resistance and outlines approaches suitable for improving therapeutic outcomes for HCC patients. | Weiwei Tang Ziyi Chen Wenling Zhang Ye Cheng Betty Zhang Fan Wu Qian Wang Shouju Wang Dawei Rong F.P.Reiter E.N.De Toni Xuehao Wang | 2020 | Signal Transduction and Targeted Therapy2020，5，1： | 19 |
| 6 | Differential Fault Attack on Camellia显示文摘 | ZHOU Yongbin WU Wenling XU Nannan FENG Dengguo | 2009 | Chinese Journal of Electronics2009，18，1： | 15 |
| 7 | Relative humidity reconstruction for northwestern China's Altay Mountains using tree-ring δ^(18)O显示文摘Relative humidity is an important factor in water and water vapor feedback cycles.In this study,we established a 222-year annual tree-ring δ^(18)O chronology for Siberian larch(Larix sibirica Ldb.)from the Altay Mountains in northwestern China.Climate response analyses revealed that the relative humidity was the primary factor limiting tree-ring δ^(18)O fractionation.Based on our analysis,tree-ring δ^(18)O can be used to reconstruct the July–August relative humidity based on both a reasonable mechanism of tree-ring δ^(18)O fractionation and a statistically significant regression model.We used this model to reconstruct variations in the July–August relative humidity,and the model explained 47.4% of the total variation in the measured relative humidity data from 1961 to 2011.The relative humidity in the study area increased from 1900 to the 1990s and decreased thereafter.Two regime-shift dry periods were detected during the study period(one from 1817 to 1830 and the other from 2004 to 2011). | Guobao Xu Xiaohong Liu Dahe Qin Tuo Chen Wenzhi Wang Guoju Wu Weizhen Sun Wenling An Xiaomin Zeng | 2014 | Chinese Science Bulletin2014，59，2： | 14 |
| 8 | Identification of aberrant cell cycle regulation in Epstein-Barr virus-associated nasopharyngeal carcinoma by cDNA microarray and gene set enrichment analysis显示文摘 | Wenling Zhang Zhaoyang Zeng Yanhong Zhou Wei Xiong Songqing Fan Lan Xiao Donghai Huang Zheng Li Dan Li Minghua Wu Xiaoling Li Shourong Shen Rong Wang Li Cao Ke Tang Guiyuan Li | 2009 | Acta Biochimica et Biophysica Sinica2009，41，5： | 11 |
| 9 | Preparation of polyclonal antibody specific for NOR1 and detection of its expression pattern in human tissues and nasopharyngeal carcinoma显示文摘包含蛋白质 1 的 Oxidored-nitro 领域(NOR1 ) 基因是首先从鼻咽的癌(NPC ) 孤立的新奇 nitroreductase 基因。它为它的 nitrosation 功能在化学致癌物的形成和 NPC 的 carcinogenesis 起一个重要作用。在鼻咽的癌细胞的野类型的 NOR1 基因的 Overexpression 是有效的禁止细胞生长和增长。在这研究,第一次,我们产生了高度特定的 NOR1 抗体并且在人的纸巾和 NPC 活体检视分析了 NOR1 分发。结果证明那 NOR1 蛋白质主要在人的鼻咽和气管的纸巾被表示。人的心,肝,怒气,胃,冒号,肾,骨胳的肌肉,胸腺,和胰都 NOR1 蛋白质是缺乏的。更重要地,我们在 NPC 纸巾执行了 NOR1 蛋白质表示的 immunohistochemistry 试金,并且结果证明那 NOR1 蛋白质在 NPC 经常是下面表示的。这些数据使 NOR1 的潜在的生理的函数的选择清楚些并且提供不可缺少的引用给 NPC 的 carcinogenesis 进程并且到识别或验证织物特定的药目标。 | Bo Xiang Mei Yi Li Wang Wei Liu Wenling Zhang Jue Ouyang Ya Peng Wenjuan Li Ming Zhou Huaying Liu Minghua Wu Rong Wan Xiaoling Li Guiyuan Li | 2009 | Acta Biochimica et Biophysica Sinica2009，41，9： | 10 |
| 10 | Identification of HBsAg-specific antibodies from a mammalian cell displayed full-length human antibody library of healthy immunized donor显示文摘肝炎 B 免疫球蛋白(HBIG ) 在肝炎 B 的管理是重要的病毒(HBV ) 感染。试图作为 HBIG 的一种选择开发 recombinant monoclonal 抗体,我们报导 HBV 表面抗原(HBsAg ) 的成功的鉴定从在哺乳动物的房间上显示的一个全身的人的抗体图书馆的特定的抗体出现。把一个生来地使免疫的施主的外部血 mononuclear 房间的全部的 RNA 用作模板,抗体全部剧目被放大。联合四方法的结扎和 Flp 调停 recombinase 的集成(Flp 在里面) 系统,我们构造了每个房间在显示了一个种仅仅抗体分子的一个哺乳动物的基于房间的、充分人的、全身的抗体显示图书馆。由屏蔽使用激活荧光的房间排序的房间图书馆(FACS ) ,八个房间克隆显示那房间表面上的 HBsAg 特定的抗体被识别。抗体基因的 DNA 顺序分析揭示了三唯一的抗体。FACS 数据显示了那荧光灯表示(FSE ) 的力量,荧光灯绑定(FSB ) 的力量和相对有约束力的能力(RBA ) 都在他们之中是不同的。这些结果由使用我们的抗体表明了那哺乳动物的显示和屏蔽站台,我们能成功地从一个使免疫的全身的抗体图书馆识别抗原特定的抗体。因此,这个平台为治疗学的抗体的开发是很有用的。 | Chang-Zheng Li Zhong-Kun Liang Zhen-Rui Chen Hai-Bo Lou Ye Zhou Zhe-Huan Zhang Fei Yu Shuwen Liu Yuanping Zhou Shuguang Wu Wenling Zheng Wanlong Tan Shibo Jiang Chen Zhou | 2012 | Cellular & Molecular Immunology2012，9，2： | 7 |
| 11 | Tuning Atomically Dispersed Fe Sites in Metal–Organic Frameworks Boosts Peroxidase‑Like Activity for Sensitive Biosensing显示文摘Although nanozymes have been widely developed,accurate design of highly active sites at the atomic level to mimic the electronic and geometrical structure of enzymes and the exploration of underlying mechanisms still face significant challenges.Herein,two functional groups with opposite electron modulation abilities(nitro and amino)were introduced into the metal–organic frameworks(MIL-101(Fe))to tune the atomically dispersed metal sites and thus regulate the enzymelike activity.Notably,the functionalization of nitro can enhance the peroxidase(POD)-like activity of MIL-101(Fe),while the amino is poles apart.Theoretical calculations demonstrate that the introduction of nitro can not only regulate the geometry of adsorbed intermediates but also improve the electronic structure of metal active sites.Benefiting from both geometric and electronic effects,the nitro-functionalized MIL-101(Fe)with a low reaction energy barrier for the HO*formation exhibits a superior POD-like activity.As a concept of the application,a nitro-functionalized MIL-101(Fe)-based biosensor was elaborately applied for the sensitive detection of acetylcholinesterase activity in the range of 0.2–50 mU mL−1 with a limit of detection of 0.14 mU mL−1.Moreover,the detection of organophosphorus pesticides was also achieved.This work not only opens up new prospects for the rational design of highly active nanozymes at the atomic scale but also enhances the performance of nanozyme-based biosensors. | Weiqing Xu Yikun Kang Lei Jiao Yu Wu Hongye Yan Jinli Li Wenling Gu Weiyu Song Chengzhou Zhu | 2020 | Nano-Micro Letters2020，12，12： | 4 |
| 12 | Design and application of 60mer oligonucleotide microarray in SARS coronavirus detection显示文摘The 60mer oligonucleotide microarray was designed and applied to detecting of SARS (severe acute res-piratory syndrome) coronavirus. Thirty 60mer specific oligos were designed to cover the whole genome of the first submit-ted coronavirus strain, according to the sequence of TOR2 (GENEBANK Accession: AY274119). These primers were synthesized and printed into a microarray with 12×12 spots. RNAs were extracted from the throat swab and gargling fluid of SARS patients and reverse-transcripted into the double strand cDNAs. The cDNAs were prepared as re-stricted cDNA fragments by the restriction display (RD) technique and labeled by PCR with the Cy5-universal primer. The labeled samples were then applied to the oligo microar-ray for hybridization. The diagnostic capability of the mi-croarray was evaluated after the washing and scanning steps. The scanning result showed that samples of SARS patients were hybridized with multiple SARS probes on the microar-ray, and there is no signal on the negative and blank controls. These results indicate that the genome of SARS coronavirus can be detected in parallel by the 60mer oligonucleotide mi-croarray, which can improve the positive ratio of the diagno-sis. The oligo microarray can also be used for monitoring the behavior of the virus genes in different stages of the disease status. | SHI Rong MA Wenli WU Qinghua ZHANG Bao SONG Yanbin GUO Qiuye XIAO Weiwei WANG Yan ZHENG Wenling | 2003 | Chinese Science Bulletin2003，48，12： | 4 |
| 13 | Isolation and Characterization of Monkeypox Virus from the First Case of Monkeypox—Chongqing Municipality,China,2022显示文摘Introduction:The first imported case of monkeypox(MPX)from the mainland of China was reported in September 2022.Herein,the study reports the isolation and characterization of MPX virus(MPXV)in this case.Methods:Clinical specimens including skin blister fluid,oropharyngeal and nasopharyngeal swabs,and blood were collected and inoculated onto Vero cells.The isolated virus was identified as MPXV using quantitative polymerase chain reaction(qPCR),cytopathic effects(CPEs),immunofluorescence assay(IFA)and transmission electron microscopy(TEM).Plaque assays were employed to quantify infectious plaque-forming units(PFUs).The plaque reduction neutralization test(PRNT)was developed to determine the neutralizing antibody(nAb)against MPXV.Results:MPXV replication was confirmed with qPCR.Typical CPEs were observed 48 h postincubation.The isolated virus was named MPXV-B.1-China-C-Tan-CQ01.IFA showed that MPXV reacted with serum of MPX case.Orthopoxvirus morphology was observed using TEM.The virus titer increased to>106 PFU/mL after three passages.The serum PRNT 50%neutralization titer(NT50)was 35 for the MPX patient 6 days after symptom onset.Discussion:The study successfully isolated the first MPXV strain in the mainland of China,MPXV-B.1-China-C-Tan-CQ01.Infectious titration and PRNT methods have been developed.The study provides key resources and technical platforms for further research as well as anti-viral drug and vaccine development against MPX. | Baoying Huang Hua Zhao Jingdong Song Li Zhao Yao Deng Wen Wang Roujian Lu Wenling Wang Jiao Ren Fei Ye Houwen Tian Guizhen Wu Hua Ling Wenjie Tan | 2022 | China CDC weekly2022，4，46： | 3 |
| 14 | Intestinal dysbiosis exacerbates the pathogenesis of psoriasis-like phenotype through changes in fatty acid metabolism显示文摘The intestinal microbiota has been associated with host immunity as well as psoriasis;however,the mechanism of intestinal microbiota regulating psoriasis needs to be demonstrated systematically.Here,we sought to examine its role and mechanism of action in the pathogenesis of psoriasis.We found that the severity of psoriasis-like skin phenotype was accompanied by changes in the composition of the intestinal microbiota.We performed co-housing and fecal microbial transplantation(FMT)experiments using the K14-VEGF transgenic mouse model of psoriasis and demonstrated that the transfer of intestinal microbiota from mice with severe psoriasis-like skin phenotype exacerbated psoriasiform skin inflammation in mice with mild symptoms,including increasing the infiltration and differentiation of Th17,and increased the abundance of Prevotella,while decreasing that of Parabacteroides distasonis,in the colon.These alterations affected fatty acid metabolism,increasing the abundance of oleic and stearic acids.Meanwhile,gentamicin treatment significantly reduced the abundance of Prevotella and alleviated the psoriasis-like symptoms in both K14-VEGF mice and imiquimod(IMQ)-induced psoriasis-like mice.Indeed,administration of oleic and stearic acids exacerbated psoriasis-like symptoms and increased Th17 and monocyte-derived dendritic cell infiltration in the skin lesion areas in vivo,as well as increased the secretion of IL-23 by stimulating DCs in vitro.At last,we found that,treatment of PDE-4 inhibitor alleviated psoriasis-like phenotype of K14-VEGF mice accompanied by the recovery of intestinal microbiota,including the decrease of Prevotella and increase of Parabacteroides distasonis.Overall,our findings reveal that the intestinal microbiota modulates host metabolism and psoriasis-like skin inflammation in mice,suggesting a new target for the clinical diagnosis and treatment of psoriasis. | Qixiang Zhao Jiadong Yu Hong Zhou Xiaoyan Wang Chen Zhang Jing Hu Yawen Hu Huaping Zheng Fanlian Zeng Chengcheng Yue Linna Gu Zhen Wang Fulei Zhao Pei Zhou Haozhou Zhang Nongyu Huang Wenling Wu Yifan Zhou Jiong Li | 2023 | Signal Transduction and Targeted Therapy2023，8，2： | 2 |
| 15 | The DBlock family of block ciphers显示文摘In this paper,we propose a new family of block ciphers named DBlock.It consists of three variants which are specified as DBlock-128,DBlock-192,and DBlock-256,respectively.DBlock-n has the equal n-bit block length and key length.The structure of DBlock successfully combines the advantages of Feistel and Type-2generalized Feistel structures together.Also,its design of round function employs different linear transforms operating on various word-sizes,which efficiently improve the diffusion effect.For key schedule of DBlock,it basically employs the same module used in encryption,except the choice of different byte permutations,which can improve its suitability for various implementation environments and also enhance its security against many cryptanalytic techniques.Our preliminary evaluation shows that DBlock can achieve enough security margin against known attacks,and it can also obtain excellent performances on various software and hardware platforms. | WU WenLing ZHANG Lei YU XiaoLi | 2015 | Science China(Information Sciences)2015，58，3： | 2 |
| 16 | Performance analysis of peak cancellation in OFDM systems显示文摘Peak cancellation is a class of peak-to-average power ratio (PAPR) reduction techniques for orthogo-nal frequency division multiplexing (OFDM).It can control the PAPR and out-of-band radiation simultaneously at the cost of additional interference.In this paper,the interference of peak cancellation is modeled and an-alyzed so as to evaluate the degradation of system performance.Furthermore,through theoretical analysis,a closed form of the BER performance is derived for OFDM with popular modulation schemes such as QPSK and 16QAM.Finally,the analytical results are verified by simulations. | XIAO Yue BAI WenLing DAN LiLin WU Gang LI ShaoQian | 2012 | Science China(Information Sciences)2012，55，4： | 2 |
| 17 | Known-key distinguishers on type-1 Feistel scheme and near-collision attacks on its hashing modes显示文摘 | Le DONG Wenling WU Shuang WU Jian ZOU | 2014 | Frontiers of Computer Science2014，8，3： | 2 |
| 18 | Neuropeptide Y gene transfection inhibits post-epileptic hippocampal synaptic reconstruction显示文摘Exogenous neuropeptide Y has antiepileptic effects; however, the underlying mechanism and optimal administration method for neuropeptide Y are still unresolved. Previous studies have used intracerebroventricular injection of neuropeptide Y into animal models of epilepsy. In this study, a recombinant adeno-associated virus expression vector carrying the neuropeptide Y gene was injected into the lateral ventricle of rats, while the ipsilateral hippocampus was injected with kainic acid to establish the epileptic model. After transfection of neuropeptide Y gene, mossy fiber sprouting in the hippocampal CA3 region of epileptic rats was significantly suppressed, hippocampal synaptophysin (p38) mRNA and protein expression were inhibited, and epileptic seizures were reduced. These experimental findings indicate that a recombinant adeno-associated virus expression vector carrying the neuropeptide Y gene reduces mossy fiber sprouting and inhibits abnormal synaptophysin expression, thereby suppressing post-epileptic synaptic reconstruction. | Fan Zhang Wenqing Zhao Wenling Li Changzheng Dong Xinying Zhang Jiang Wu Na Li Chuandong Liang | 2013 | Neural Regeneration Research2013，8，17： | 2 |
| 19 | Neuropeptide Y protects cerebral cortical neurons by regulating microglial immune function显示文摘Neuropeptide Y has been shown to inhibit the immunological activity of reactive microglia in the rat cerebral cortex, to reduce N-methyl-D-aspartate current(INMDA) in cortical neurons, and protect neurons. In this study, after primary cultured microglia from the cerebral cortex of rats were treated with lipopolysaccharide, interleukin-1β and tumor necrosis factor-α levels in the cell culture medium increased, and mRNA expression of these cytokines also increased. After primary cultured cortical neurons were incubated with the lipopolysaccharide-treated microglial conditioned medium, peak INMDA in neurons increased. These effects of lipopolysaccharide were suppressed by neuropeptide Y. After addition of the neuropeptide Y Y1 receptor antagonist BIBP3226, the effects of neuropeptide Y completely disappeared. These results suggest that neuropeptide Y prevents excessive production of interleukin-1β and tumor necrosis factor-α by inhibiting microglial reactivity. This reduces INMDA in rat cortical neurons, preventing excitotoxicity, thereby protecting neurons. | Qijun Li Changzheng Dong Wenling Li Wei Bu Jiang Wu Wenqing Zhao | 2014 | Neural Regeneration Research2014，9，9： | 1 |
| 20 | Atomically dispersed N-coordinated Fe-Fe dual-sites with enhanced enzyme-like activities显示文摘Replacement of enzymes with nanomaterials such as atomically dispersed metal catalysts is one of the most crucial steps in addressing the challenges in biocataiysis.Despite the breakthroughs of single-atom catalysts in enzyme-mimicking,a fundamental investigation on the development of an instructional strategy is still required for mimicking biatomic/multiatomic active sites in natural enzymes and constructing synergistically enhanced metal atom active sites.Herein,Fe_(2)NC catalysts with atomically dispersed Fe-Fe dual-sites supported by the metal-organic frameworks-derived nitrogen-doped carbon are employed as biomimetic catalysts to perform proof-of-concept investigation.The effect of Fe atom number toward typical oxidase(cytochrome C oxidase,NADH oxidase,and ascorbic acid oxidase)and peroxidase(NADH peroxidase and ascorbic acid peroxidase)activities is systematically evaluated by experimental and theoretical investigations.A peroxo-like O_(2) adsorption in Fe_(2)NC nanozymes could accelerate the O-O activation and thus achieve the enhanced enzyme-like activities.This work achieves the vivid simulation of the enzyme active sites and provides the theoretical basis for the design of high-performance nanozymes.As a concept application,a colorimetric biosensor for the detection of S^(2-) in tap water is established based on the inhibition of enzyme-like activity of Fe_(2)NC nanozymes. | Lei Jiao Wei Ye Yikun Kang Yu Zhang Weiqing Xu Yu Wu Wenling Gu Weiyu Song Yujie Xiong Chengzhou Zhu | 2022 | Nano Research2022，15，2： | 1 |
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