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| 1 | Association of polymorphisms of Nramp1 gene with immune function and production performance of large white pig显示文摘The present research was designed to study the association of polymorphism of natural resistance-associated macrophage protein1 (Nramp1) with some immune function and the production performance in Large White pig. The PCR-RFLP technique was applied to analyze the correlation between the polymorphisms of Nramp1 gene and immune function [value of Polymorphonuclear Leukocytes (PMN) obtained by Nitroblue Tetrazolium (NBT) Reduction and effect of Cytotoxin in Monocyte] and production performance in 165 Large White pigs. The results showed that there was one Nde I restriction locus in Large White pig, and both values of PMN by NBT Reduction and effect of Cytotoxin in Monocyte in genotype BB were higher than those in genotype AB (P<0.05). Simultaneously, the weight of 180-day-old pigs with genotype BB was higher than that with genotype AB (P<0.05). The results indicated that there was a significant correlation between different genotypes of Nramp1 gene and Immune function and production performance, and it can be regarded as a candidate gene of disease resistance. All these results provide valuable reference to further studies of pig disease resistance. | Hongmei Wu Duxue Cheng Lixian Wang | 2008 | Journal of Genetics and Genomics2008，35，2： | 12 |
| 2 | NLRP3-dependent pyroptosis is required for HIV-1 gp120- induced neuropathology显示文摘The human immunodeficiency virus-1(HIV-1)envelope protein gp120 is the major contributor to the pathogenesis of HIVassociated neurocognitive disorder(HAND).Neuroinflammation plays a pivotal role in gp120-induced neuropathology,but how gp120 triggers neuroinflammatory processes and subsequent neuronal death remains unknown.Here,we provide evidence that NLRP3 is required for gp120-induced neuroinflammation and neuropathy.Our results showed that gp120-induced NLRP3-dependent pyroptosis and IL-1βproduction in microglia.Inhibition of microglial NLRP3 inflammasome activation alleviated gp120-mediated neuroinflammatory factor release and neuronal injury.Importantly,we showed that chronic administration of MCC950,a novel selective NLRP3 inhibitor,to gp120 transgenic mice not only attenuated neuroinflammation and neuronal death but also promoted neuronal regeneration and restored the impaired neurocognitive function.In conclusion,our data revealed that the NLRP3 inflammasome is important for gp120-induced neuroinflammation and neuropathology and suggest that NLRP3 is a potential novel target for the treatment of HAND. | Xiaolong He Weijun Yang Zhijie Zeng Yi Wei Jie Gao Bao Zhang Li Li Liqun Liu Yu Wan Qing Zeng Zelong Gong Liting Liu Hanyun Zhang Yubin Li Shaojie Yang Tongtong Hu Lixian Wu Eliezer Masliah Shenghe Huang Hong Cao | 2020 | Cellular & Molecular Immunology2020，17，3： | 11 |
| 3 | Downregulation of cyclooxygenase-1 is involved in gastric mucosal apoptosis via death signaling in portal hypertensive rats显示文摘门高血压(PHT ) gastropathy 是肝肝硬化的经常的复杂并发症,带之一从肝硬化死亡引起。Apoptosis 广泛地被认为从坏死的房间死亡是房间死亡和一个不同实体的一个活跃精力依赖者模式。胃的 mucosal apoptosis 是否涉及 PHT gastropathy,是不清楚的。通过 cyclooxygenase (艇长) 生产的前列腺素(PG ) 被认为从损害和 apoptosis 在胃肠的 mucosa 的保护起一个关键作用。然而,在 PHT gastropathy 的艇长的角色仍然不清楚地被理解。这研究的目的是调查(1 ) 胃的 mucosal apoptosis 是否涉及 PHT gastropathy,(2 ) 艇长的 downregulation 贡献这 apoptosis。在这研究,当 mucosal 增长在 PHT 老鼠被禁止时,我们证明胃的 mucosal apoptosis 显著地被增加。胃的 mucosal COX-1 显著地在 mRNA 和蛋白质层次被压制,并且 PGE2 在 PHT 老鼠被减少。进一步, PGE2 处理在 PHT 老鼠压制了胃的 mucosal apoptosis。然而,胃的 mucosal COX-2 层次没在假冒操作老鼠和 PHT 老鼠之间不同。肿瘤坏死因素的胃的 mucosal 层次 --(TNF-) 并且船边交货 ligand,然而并非 TNF 相关的导致 apoptosis ligand,被增加,并且激活的 caspase-8 和 caspase-3 层次是在 PHT 老鼠的 upregulated。到 cytosol 的从线粒体的细胞色素 c 的版本没在 PHT 老鼠被观察。我们的数据显示 COX-1 的 downregulation 经由死亡涉及胃的 mucosal apoptosis 调停信号的类型 -- 我在 PHT 老鼠的房间死亡。 | Bin Wu Lixian Zeng Ying Lin Zhuofu Wen Guihua Chen Ryuichi Iwakiri Kazuma Fujimoto | 2009 | Cell Research2009，19，11： | 6 |
| 4 | MATHEMATICAL MODEL OF OVERLAND FLOW AND MECHANISM OF SOIL CONSERVATION FOR FORESTED STEEP HILLSLOPE--(II)Mechanism of Soil Conservation Over Steep Hillslope显示文摘MATHEMATICALMODELOFOVERLANDFLOWANDMECHANISMOFSOILCONSERVATIONFORFORESTEDSTEEPHILLSLOPE--(II)MechanismofSoilConservationOverSt... | WU Changwen and WANG Lixian ( Science. Nanchang College of Water Resources and Hydro Power, 330029)( Dept of Soil and Water Conservation, Beijing Forestry University, 100083,PRC) | 1995 | International Journal of Sediment Research1995，10，2： | 4 |
| 5 | Sliding mode H_∞ control for a class of uncertain nonlinear state-delayed systems显示文摘A new proportional-integral (PI) sliding surface is designed for a class of uncertain nonlinear state-delayed systems. Based on this, an adaptive sliding mode controller (ASMC) is synthesized, which guarantees the occurrence of sliding mode even when the system is undergoing parameter uncertainties and external disturbance. The resulting sliding mode has the same order as the original system, so that it becomes easy to solve the H_∞ control problem by designing a memoryless H_∞ state feedback controller. A delay-dependent sufficient condition is proposed in terms of linear matrix inequalities (LMIs), which guarantees the sliding mode robust asymptotically stable and has a noise attenuation level γ in an H_∞ sense. The admissible state feedback controller can be found by solving a sequential minimization problem subject to LMI constraints by applying the cone complementary linearization method. This design scheme combines the strong robustness of the sliding mode control with the H_∞ norm performance. A numerical example is given to illustrate the effectiveness of the proposed scheme. | Wu Ligang Wang Changhong Gao Huijun Zhang Lixian | 2006 | Journal of Systems Engineering and Electronics2006，17，3： | 4 |
| 6 | LOCAL FLEXIBILITY BEHAVIOR OF TUBULAR JOINTS AND ITS EFFECT ON GLOBAL ANALYSIS OF TUBULAR STRUCTURES显示文摘In the tubular frames with simple joints, the joints may show considerable local flexibility, which causes excessive deformation and affects internal force distributions. It is useful and important to study turbular joint flexibility as well as the effect of joint local flexibility on the global behavior of tubular structure for the design and building of offshore platforms. A new method for computing the stiffness coefficients of simple tubular joints is developed in this paper based on two previous computing models. According to this method, the local flexibility of T, Y, TY tubular joints are computed. This method has merits of clear physical sense, simplicity of computations and less c. p. u. time. The effect of flexibility of tubular joints on the global behavior of framed tubular structures is investigated by comparing the computed results of tubular structures with flexible tubular joints with those with rigid connections. The comparison shows that the consideration of local flexibility of tubular joints and its effect on the stress analysis of offshore structures has its practical significance. | Yang, Lixian Chen, Tieyun Wu, Shuiyun | 1990 | China Ocean Engineering1990，5，4： | 3 |
| 7 | Identification and bioinformatics analysis of lactate dehydrogenase genes from Echinococcus granulosus显示文摘Objective:To identify full length cDNA sequence of lactate dehydrogenase(LDH) from adult Echinococcus granulosus(E.granulosus) and to predict the structure and function of its encoding protein using bioinformatics methods.Methods:With the help of NCBI,EMBI, Expasy and other online sites,the open reading frame(ORF),conserved domain,physical and chemical parameters,signal peptide,epitope,topological structures of the protein sequences were predicted and a homology tertiary structure model was created:Vector NT1 software was used for sequence alignment,phylogenetic tree construction and tertiary structure prediction. Results:The target sequence was 1 233 bp length with a 996 bp biggest ORF encoding 331 amino acids protein with typical L-LDH conserved domain.It was confirmed as full length cDNA of LDH from E.granulosus and named as EgLDH(GenBank accession number:HM748917).The predicted molecular weight and isoelectric point of the deduced protein were 3 5516.2Da and 6.32 respectively.Compared with LDHs from Taenia solium,Taenia saginata asiatica,Spirometra erinaceieuropaei.Schistosoma japonicum,Clonorchis sinensis and human,it showed similarity of 86% ,85% ,55% ,58% ,58% and 53% ,respectively.EgLDH contained 3 putative transmembrane regions and 4 major epitopes(54aa-59aa.81aa-87aa,97aa-102aa,307aa-313aa),the latter were significant different from the corresponding regions of human LDH.In addition,some NAD and substrate binding sites located on epitopes 54aa-59aa and 97aa-102aa,respectively.Tertiary structure prediction showed that 3 key catalytic residues 105R,165D and 192H forming a catalytic center near the epitope 97aa-102aa,most NAD and substrate binding sites located around the center.Conclusions:The full length cDNA sequences of EgLDH were identified.It encoded a putative transmembrane protein which might be an ideal target molecule for vaccine and drugs. | Gang Lu Yajun Lu Lihua Li Lixian Wu Zhigang Fan Dazhong Shi Hu Wang Xiumin Han | 2010 | Asian Pacific Journal of Tropical Medicine2010，3，10： | 2 |
| 8 | Dual Inhibition of Bcr-Abl and Hsp90by C086 Potently Inhibits the Proliferation of Imatinib-Resistant CML Cells显示文摘 | Lixian Wu Jing Yu Ruijia Chen | 2015 | Clinical Cancer Research2015，21，4： | 1 |
| 9 | Nitrogen-doped hierarchical porous carbon materials derived from diethylenetriaminepentaacetic acid (DTPA) for supercapacitors显示文摘Nitrogen-doped porous carbon materials(NPCs) have been successfully fabricated by a simple one-step pyrolysis of diethylenetriaminepentaacetic acid(DTPA) in the presence of KOH. The as-synthesized NPCs displayed a high specific surface area(3214 m^2 g^(-1)) and a well-defined porous structure when the annealing temperature reached 800 ℃, which showed superior electrochemical performance as supercapacitor electrode materials. Electrochemical tests showed that the NPCs achieved an impressive specific capacitance of 323 F g^(-1) at a current density of 0.5 A g^(-1) in 6 M KOH aqueous solution and an outstanding cycle stability, negligible specific capacitance decay after 5000 cycles at 10 A g^(-1). This strategy offered a new insight into the preparation of novel carbon materials for the advanced energy storage devices, such as supercapacitors, fuel cells and lithium ion batteries. | Tinghuan Wu Lixian Sun Fen Xu Dan Cai | 2018 | Journal of Materials Science & Technology2018，34，12： | 1 |
| 10 | The downregulation of ErbB3 binding protein 1 (EBP1) is associated with poor prognosis and enhanced cell proliferation in hepatocellular carcinoma显示文摘 | Baoying Hu Yicheng Xiong Runzhou Ni Lixian Wei Dawei Jiang Gang Wang Di Wu Tianxin Xu Fengbo Zhao Mingyan Zhu Chunhua Wan | 2014 | 2014 (1-2)2014，，1： | 1 |
| 11 | Effect of propofol on the levels of neurotransmitters in normal human brain: A magnetic resonance spectroscopy study显示文摘 | Hui Zhang Wei Wang Wei Gao Yali Ge Jinsong Zhang Shengxi Wu Lixian Xu | 2009 | Neuroscience Letters2009，，3： | 1 |
| 12 | Identification,structure and function of a novel tetraspaninhoniologue from Spirometra erinaceieuropaei显示文摘Objective:To identify a full length cDNA sequence of a novel tetraspanin(TSP) homologue from Spirometra erinaceieuropaei and to predict the structure and function of its encoding protein using bioinformatics methods.Methods:Using the NCBI,EMBI,Expasy and other online sites, the open reading frame(ORF),conserved domain,physical and chemical parameters,signal peptide,transmembrane domain,epitope,topological structures of the protein sequences were predicted.And Vector NTI software was used for multiple sequence alignment and phylogenetic tree construction.Results:’Hie target sequence was 1 132 hp length with a 681 hp biggest ORF encoding 226 amino acids protein with typical TSP conserved domain.It was confirmed as full length cDNA of TSP16 from Spirometra erinaceieuropaei and named as SeTSP16 (GenBank accession number:JF728872).The predicted molecular weight and isoelectric point of the deduced protein were 24 750.5 Da and 7.88 Da,respectively.Compared with TSP16s from Schistosoma japonicum and Schistosoma mansoni.it showed similarity of 59%and 59%, respectively.SeTSP16 contained four transmembrane domains(TM 1-4),intracellular N and C-termini,one short small extracellular loop and one large extracellular loop.Four major epitopes that were significant different from the corresponding epitope regions of TSP16 from Schistosoma mansoni and Schistosoma japonicum were predicted.Conclusions:The full length cDNA sequences of SeTSP16 arc identified.It encodes a transmembrane protein which might be an ideal diagnosis antigen and target molecule for antiparasitic drugs. | Fu Qiongyao Lu Yajun Rao Langyu Chen Jinglong Wu Qiang Cai Qunfang Li Lihua Wu Lixian Lu Gang | 2011 | Asian Pacific Journal of Tropical Medicine2011，4，9： | 1 |
| 13 | Syntheses of CuO nanostructures in ionic liquids显示文摘A simple and efficient approach is developed to fabricate single-crystalline CuO nanostructures through an ionic liquid assisted one-step low-temperature solid-state route.Both nanoparticles(5 nm in size)and nanorods(5-10 nm in diameter and 50-100 nm in length)of monoclinic CuO were obtained. These synthesized CuO nanostructures were characterized by X-ray diffraction(XRD),transmission electron microscopy(TEM),selected area electron diffraction(SAED),X-ray photoelectron spectros- copy(XPS),energy dispersive spectroscopy(EDS)and nitrogen adsorption analysis.The morpholo- gies of the nanostructures can be controlled by tuning the amount of NaOH and ionic liquids.The growth mechanism of CuO nanostructures is investigated. | WANG Li ZHAO Bin YUAN ZhongYong ZHANG XueJun Wu QingDuan CHANG LiXian ZHENG WenJun | 2007 | Science China Chemistry2007，50，1： | 1 |
| 14 | Effect of continuous hemofiltration on hemodynamics, lung inflammation and pulmonary edema in a canine model of acute lung injury显示文摘 | Xiao Su Chunxue Bai Qunying Hong Duming Zhu Lixian He Jianping Wu Feng Ding Xiaohui Fang Michael A. Matthay | 2003 | Intensive Care Medicine2003，，11： | 1 |
| 15 | Rambutan-like hierarchically porous carbon microsphere as electrodematerial for high-performance supercapacitors显示文摘Used as high-performance electrodes,both structural and compositional alterations of carbon materials play very important roles in energy conversion/storage devices.Especially in supercapacitors,hierarchical pores and heteroatom doping in carbon materials are indispensable.Here the rambutan-like hierarchically porous carbon microspheres(PCMs)have been constructed via a hydrothermal treatment,followed by carbonization/activation.The hierarchically porous microstructure is composed of three-dimensional porous carbon networks,which give rise to a large surface area.Moreover,N and O functional groups are introduced in the as-prepared samples,which could generate the extra pseudocapacitance.Benefitting from the interconnected hierarchical and open structure,PCM exhibits outstanding capacitive performance,for example,superior specific capacitance and rate capability(397 and 288 F g^(−1) at 0.5 and 20A g^(−1),respectively),as well as long cycling stability(about 95%capacitance retention after 10,000 cycles).These encouraging results may pave an efficient way to fabricate advanced supercapacitors in the future. | Chunfeng Shao Shujun Qiu Guiming Wu Boyang Cui Hailiang Chu Yongjin Zou Cuili Xiang Fen Xu Lixian Sun | 2021 | Carbon Energy2021，3，2： | 1 |
| 16 | Single-cell RNA Sequencing Reveals Thoracolumbar Vertebra Heterogeneity and Rib-genesis in Pigs显示文摘Development of thoracolumbar vertebra(TLV)and rib primordium(RP)is a common evolutionary feature across vertebrates,although whole-organism analysis of the expression dynamics of TLV-and RP-related genes has been lacking.Here,we investigated the single-cell transcriptome landscape of thoracic vertebra(TV),lumbar vertebra(LV),and RP cells from a pig embryo at 27 days post-fertilization(dpf)and identified six cell types with distinct gene expression signatures.In-depth dissection of the gene expression dynamics and RNA velocity revealed a coupled process of osteogenesis and angiogenesis during TLV and RP development.Further analysis of cell type-specific and strand-specific expression uncovered the extremely high level of HOXA103′-UTR sequence specific to osteoblasts of LV cells,which may function as anti-HOXA10-antisense by counteracting the HOXA10-antisense effect to determine TLV transition.Thus,this work provides a valuable resource for understanding embryonic osteogenesis and angiogenesis underlying vertebrate TLV and RP development at the cell type-specific resolution,which serves as a comprehensive view on the transcriptional profile of animal embryo development. | Jianbo Li Ligang Wang Dawei Yu Junfeng Hao Longchao Zhang Adeniyi C.Adeola Bingyu Mao Yun Gao Shifang Wu Chunling Zhu Yongqing Zhang Jilong Ren Changgai Mu David M.Irwin Lixian Wang Tang Hai Haibing Xie Yaping Zhang | 2021 | Genomics, Proteomics & Bioinformatics2021，19，3： | 1 |
| 17 | Effect of artmether,hemin and Fe^(3+) on recombinant lactate dehydrogenase from Schistosoma japonicum显示文摘Objective:To explore antischitosome effects of artemether,hemin and Fe on S/LDH.Methods: Enzyme activity of rS/LDH was assayed in the standard reaction system by adding different concentration of reagents(0.00-0.10 mM artemether,0.00-0.02 mM hemin,0.00-0.50 mM Fe3+). Same solvents of the each reagent were used as control.Results:There was no enzyme activity inhibition observed at 0.10 mM artemther:obivious inhibition for lactate oxidation reaction and pyruvate reduction reaction were detected at 0.002 mM and 0.004 mM of hemin,respectively: comparing with that of the control(P<0.05).The relative enzymatic activity inhibitions for pyruvate reduction reaction and lactate oxidation reaction at 0.02 mM hemin were 93.48%and 100.00%,respectively,comparing with that of the control(P<0.01):both pyruvate reduction and lactate oxidation reaction were inhibited completely at 0.50 mM Fe3+,comparing with that of the control(P<0.01).Conclusions:The results implied that SjLDH was not the direct molecular target of artemether.Hemin and Fe are inhibitors of SjLDH. | Gang Lu Xuchu Hu Can Huang Yajun Lu Lixian Wu Lihua Li Jing Xu Xinbing Yu | 2010 | Asian Pacific Journal of Tropical Medicine2010，3，12： | 1 |
| 18 | Effect of continuous hemofiltration on hemodynamics, lung inflammation and pulmonary edema in a canine model of acute lung injury显示文摘 | Xiao Su Chunxue Bai Qunying Hong Duming Zhu Lixian He Jianping Wu Feng Ding Xiaohui Fang Michael A. Matthay | 2003 | Intensive Care Medicine2003，，11： | 1 |
| 19 | DNAH2 facilitates the homologous recombination repair of Fanconi anemia pathway through modulating FANCD2 ubiquitination显示文摘Fanconi anemia(FA),an X-linked genetic or autosomal recessive disease,exhibits complicated pathogenesis.Previously,we detected the mutated Dynein Axonemal Heavy Chain 2(DNAH2)gene in 2 FA cases.Herein,we further investigated the potential association between DNAH2 and the homologous recombination repair pathway of FA.The assays of homologous recombination repair,mitomycin C(MMC)sensitivity,immunofluorescence,and ubiquitination modification were performed in U2OS and DR-U2OS cell lines.In MMC-treated U2OS cells,the downregulation of the DNAH2 gene increased the sensitivity of cells to DNA inter-strand crosslinks.We also observed the reduced enrichment of FANCD2 protein to DNA damage sites.Furthermore,the ubiquitination modification level of FANCD2 was influenced by the deficiency of DNAH2.Thus,our results suggest that DNAH2 may modulate the cell homologous recombination repair partially by increasing the ubiquitination and the enrichment to DNA damage sites of FANCD2.DNAH2 may act as a novel co-pathogenic gene of FA patients. | Lixian Chang Xingjie Gao Yuxia Wang Chunmin Huang Min Gao Xiaomin Wang Chao Liu Wenqi Wu Wenbin An Yang Wan Aoli Zhang Yingchi Zhang Weiping Yuan Xiaofan Zhu | 2021 | Blood Science2021，3，3： | 0 |
| 20 | Mblk-1 regulates sugar responsiveness in honey bee(Apis mellifera)foragers显示文摘Brain transcriptional regulatory network for behavior demonstrates that brain gene expression in the honey bee can be accurately predicted from the expression transcription factors(TFs),but roles for specific TFs are less understood.Mushroom bodies(MBs)are important for learning,memory and sensory integration in the honey bee brain.A TFs,Mblk-1,expressed preferentially in the large-type Kenyon cells of the honeybee MBs is predicted to be involved in brain function by regulating transcription of its target genes in honey bee.However,its function and the mechanism of regulation in behavior of honey bee is still obscure.Here we show that Mblk-1 had significantly higher expression in the brains of forager bees relative to nurse bees.Mblk-1 was significantly inhibited in bees fed small interfering RNA.In addition,inhibition of Mblk-1 decreased sucrose responsiveness in foragers.Finally,we determined that Mblk-1 regulated the messenger RNA of AmGRl.These findings suggest that Mblk-1 may target AmGRl to regulate the sucrose responsiveness of foragers. | Fang Liu Lixian Wu Yuan Zhang Qiang Li Liangbin Li Zachary Y.Huang Hongxia Zhao | 2022 | Insect Science2022，29，3： | 0 |
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