|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Monoamine alterations and rotational asymmetry in a rat model of Parkinson's disease following lateral ventricle transplantation of human amniotic epithelial cells显示文摘BACKGROUND: Human amniotic epithelial cells (HAECs) can differentiate into neurons, astrocytes,and oligodendrocytes. They biologically secrete many active neurotrophins and have the capacity tometabolize dopamine enzymes. These features underlie a theoretical basis for the treatment ofParkinson's disease (PD).OBJECTIVE: To investigate the survival and differentiation of transplanted HAECs in the lateralventricle of PD model rats, and to explore its effect on circling behavior, as well as levels ofdopamine (DA), the metabolite homovanillic acid, dihydroxyphenyl acetic acid,5-hydroxyindoleacetic acid, and 5-hydroxytryptamine in the striatum.DESIGN, TIME AND SETTING: A randomized, controlled, animal study was performed at theInstitute of Biochemistry and Cell Biology, Shanghai Institute for Biological Sciences, ChineseAcademy of Sciences, and Shanghai Celstar Institute of Biotechnology from May 2007 to December2008.MATERIALS: HAECs were derived from the placental chorion following caesarean delivery at theShanghai International Maternal and Child Health Hospital. 6-hydroxydopamine (6-OHDA), andmouse anti-human Vimentin monoclonal antibody were purchased from Sigma, USA; mouseanti-human nestin and tyrosine hydroxylase (TH) monoclonal antibodies were purchased fromChemicon, USA.METHODS: A total of 114 healthy, adult, Sprague Dawley rats were randomly assigned to twogroups: PD model [n = 90, stereotactic microinjection of 2 μL 6-OHDA (3.5 μg/μL) into the striatum]and control (n = 24, no treatment). The 51 successful PD model rats were randomly divided into 3subgroups (n = 17): HAEC, PBS, and model. The HAEC and PBS groups were respectively injectedwith 10 μL PBS solution containing 1 x 105/mL HAECs or 10 μL PBS into the lateral ventricle. Themodel group was not treated.MAIN OUTCOME MEASURES: TH protein expression in the striatum was evaluated byimmunohistochemistry 5 weeks after HAEC transplantation. At 10 weeks, HAEC survival in thelateral ventricle was investigated by immunofluorescent staining; differentiation of HAECs in thelateral and third ventricles was examined by TH immunohistochemistry; concentrations of DA,homovanillic acid, dihydroxyphenyl acetic acid, 5-hydroxyindoleacetic acid, and 5-hydroxytryptaminein the striatum, as well as DA concentration in the cerebrospinal fluid, were measured withhigh-performance liquid chromatography-electrochemical detection. Circling behavior of PD modelrats was consecutively observed for 10 weeks following intraperitoneal injection of amphetamine 1week after successful model establishment.RESULTS: In the HAEC group, the number of TH-positive cells significantly increased in thestriatum, and circling behavior significantly decreased, compared with the PBS and model groups(P < 0.01). In addition, monoamine concentrations in the striatum, as well as DA concentrations inthe cerebrospinal fluid, significantly increased, compared with the PBS group (P < 0.05-0.01 ).Moreover, a large number of nestin-, vimentin-, and TH-positive cells were observed in the lateraland third ventricles following HAEC injection. CONCLUSION: HAECs survived for 10 weeks with no overgrowth following transplantation into thelateral ventricle of PD model rats. Moreover, the cells differentiated into dopaminergic neurons,which increased DA secretion. HAEC transplantation improved cycling behavior in PD model rats. | Shouru Xue Xinxin Yang Wanli Dong Guozhen Hui Lihe GUO | 2009 | Neural Regeneration Research2009，4，12： | 6 |
| 2 | Therapeutic effects of human amniotic epithelial cell transplantation on double-transgenic mice co-expressing APPswe and PS1ΔE9-deleted genes显示文摘Human amniotic epithelial cells (HAECs), which exhibit characteristics of embryonic and pluripotent stem cells, could be utilized for cell therapy without legal or ethical problems. Double-transgenic (TG) mice (n=20) and wild-type (WT) mice (n=20) were randomly assigned to two groups, respectively. The transplantation group was treated with HAECs and the control group with PBS. A six-radial arm water maze was used to assess spatial memory. Immunofluorescence was utilized to track HAEC survival. Immunohistochemistry was used to determine octamer-binding protein 4 (oct-4) and nanog expression in the HAECs. High-performance liquid chromatography (HPLC) was used to measure acetylcholine levels in the hippocampus. The density of cholinergic neurons in the basal forebrain and nerve fibers in the hippocampus was measured following acetylcholinesterase staining. Results showed that transplanted HAECs survived for at least eight weeks and migrated to the third ventricle without immune rejection. Graft HAECs also expressed the specific stem cell markers oct-4 and nanog. Compared with the control group, HAEC transplantation significantly ameliorated spatial memory deficits in TG mice, as well as increased acetylcholine levels and the number of hippocampal cholinergic neurites. Intracerebroventricular HAEC transplantation improved spatial memory in double-TG mice, and results suggested that increased acetylcholine levels in the hippocampus, released by surviving cholinergic neurites, were responsible for this improvement. | XUE ShouRu CHEN ChongFang DONG WanLi HUI GuoZhen LIU TianJun GUO Lille | 2012 | Science China(Life Sciences)2012，55，2： | 3 |
| 3 | Unsupervised image-to-image translation by semantics consistency and self-attention显示文摘Unsupervised image-to-image translation is a challenging task for computer vision. The goal of image translation is to learn a mapping between two domains, without corresponding image pairs. Many previous works only focused on image-level translation but ignored image features processing, which led to a certain semantics loss, such as the changes of the background of the generated image, partial transformation, and so on. In this work, we propose a method of image-to-image translation based on generative adversarial nets(GANs). We use autoencoder structure to extract image features in the generator and add semantic consistency loss on extracted features to maintain the semantic consistency of the generated image. Self-attention mechanism at the end of generator is used to obtain long-distance dependency in image. At the same time, as expanding the convolution receptive field, the quality of the generated image is enhanced. Quantitative experiment shows that our method significantly outperforms previous works. Especially on images with obvious foreground, our model shows an impressive improvement. | ZHANG Zhibin XUE Wanli FU Guokai | 2022 | Optoelectronics Letters2022，18，3： | 2 |
| 4 | An Improved Item-based Collaborative Filtering Recommendation Algorithm显示文摘 | LI Xue ZUO Wanli HE Fengling | 2009 | Journal of Computer Research and De- velopment2009，46，： | 1 |
| 5 | PD-L1 is increased in the spinal cord and infiltrating lymphocytes in experimental allergic encephalomyelitis显示文摘Experimental allergic encephalomyelitis is a mouse model of human multiple sclerosis with similar pathology and pathogenesis.Th1 cells play an important role in the pathogenesis of experimental allergic encephalomyelitis.This study determined the potential effect of programmed cell death 1ligand 1 in the pathogenesis of experimental allergic encephalomyelitis induced by injecting myelin oligodendrocyte glycoprotein,complete Freund’s adjuvant and Bordetella pertussis toxin into C57BL/6J mice.Experimental allergic encephalomyelitis mice developed disease and showed inflammatory changes in the central nervous system by hematoxylin-eosin staining of spinal cord pathological sections,demyelination by Luxol fast-blue staining and clinical manifestations.The expression of programmed cell death 1 ligand 1 in mice was detected by immunohistochemistry,flow cytometry and western blot analysis.The expression of programmed cell death 1 ligand 1 in the spinal cord and splenocytes of mice was significantly increased compared with normal mice.Our findings suggest the involvement of programmed cell death 1 ligand 1 in the pathogenesis of experimental allergic encephalomyelitis and suggest this should be studied in multiple sclerosis. | Min Li Jiandong Jiang Bing Fu Jiechun Chen Qun Xue Wanli Dong Yanzheng Gu Lingtao Tang Limin Xue Qi Fang Mingyuan Wang Xueguang Zhang | 2013 | Neural Regeneration Research2013，8，35： | 1 |
| 6 | An improved item-based collaborative filtering recommendation algorithm显示文摘 | Li Xue Zuo Wanli He Fengling | 2009 | Journal of Computer Research and Development2009，，： | 1 |
| 7 | Engineering white blood cell membrane-camouflaged nanocarriers for inflammation-related therapeutics显示文摘White blood cells(WBCs)play essential roles against inflammatory disorders,bacterial infections,and cancers.Inspired by nature,WBC membrane-camouflaged nanocarriers(WBC-NCs)have been developed to mimic the“dynamic”functions of WBCs,such as transendothelial migration,adhesion to injured blood vessels,etc,which make them promising for diverse medical applications.WBC-NCs inherit the cell membrane antigens of WBCs,while still exhibiting the robust inflammation-related therapeutic potential of synthetic nanocarriers with excellent(bio)physicochemical performance.This review summarizes the proposed concept of cell membrane engineering,which utilizes physical engineering,chemical modification,and biological functionalization technologies to endow the natural cell membrane with abundant functionalities.In addition,it highlights the recent progress and applications of WBC-NCs for inflammation targeting,biological neutralization,and immune modulation.Finally,the challenges and opportunities in realizing the full potential of WBC-NCs for the manipulation of inflammation-related therapeutics are discussed. | Wanli Song Pengfei Jia Yaping Ren Junmiao Xue Bingqian Zhou Xinkai Xu Yansheng Shan Jing Deng Qihui Zhou | 2023 | Bioactive Materials2023，，5： | 1 |
| 8 | Effect of zinc, selenium and germanium on tile immunity of mice 显示文摘 | Xue Wanli He Lidan Li Xuanshe | 2000 | Xi'' an Yike Daxue Xuebao2000，21，1： | 1 |
| 9 | Linguistic Steganalysis via Fusing Multi-Granularity Attentional Text Features显示文摘Deep learning based language models have improved generation-based linguistic steganography,posing a huge challenge for linguistic steganalysis.The existing neural-network-based linguistic steganalysis methods are incompetent to deal with complicated text because they only extract single-granularity features such as global or local text features.To fuse multi-granularity text features,we present a novel linguistic steganalysis method based on attentional bidirectional long-shortterm-memory(BiLSTM) and short-cut dense convolutional neural network(CNN).The BiLSTM equipped with the scaled dot-product attention mechanism is used to capture the long dependency representations of the input sentence.The CNN with the short-cut and dense connection is exploited to extract sufficient local semantic features from the word embedding matrix.We connect two structures in parallel,concatenate the long dependency representations and the local semantic features,and classify the stego and cover texts.The results of comparative experiments demonstrate that the proposed method is superior to the state-of-the-art linguistic steganalysis. | WEN Juan DENG Yaqian PENG Wanli XUE Yiming | 2023 | Chinese Journal of Electronics2023，32，1： | 0 |
| 10 | EFFECT OF EPIMEDIUM FLAVONE TOTAL(EFT) ON THE IMMUNOFUNCTION OF EHRLICH ASCITES CANCER(EAC) BEARING MICE显示文摘Effect of Epimedium Flavone Total(EFT) on the immunofunction of mice which was burdened with Ehrlich Ascites Cancer (EAC) was studied. The results showed that EFT could increase phagocytosis activity of macrophagocyte (P<0 05), promote lymphocyte transformation rate (P<0 01), increased the sero antibody level meanly (P<0 05), low dosage group could increased Plaque Forming Cell (PFC) signficantly (P<0 05), raise the ratio of spleen to body (P<0 05) of EAC bearing mice. EFT may be enhance burdened mice’s immunofunction. Low dosage can improve sero antibody level. Effect on regulating cellular immunofunction are related to dosage. EFT may be inhibit the growth of EAC cells to some degree. | Hao Binghua,Tao Ming,He Liduan,Xue Wanli, Li Xuanshe,Zhang Jingtian Department of Microbiology and Immunology, College of Public Health, Xi′an Medical University, Xi′an 710061 | 1999 | Journal of Pharmaceutical Analysis1999，13，2： | 0 |
| 11 | Element migration and phase distribution characteristics during crystallization of rare earth phase in rare earth slag显示文摘The crystallization experiment of molten rare earth(RE)slag under different cooling rates was carried out.The characteristics of element migration and phase distribution during RE phase crystallization were studied by using different equipment.The experimental results show that there are two RE phases in the RE slag,namely(Ca,Ce,La)_(5)(SiO_(4))6F and(Ca,Ce,La,Mg)_(3)(Ti,Al,Nb)_(2)O_(7).During the cooling crystallization process of molten RE slag,Ca and P elements in the RE phase of(Ca,Ce,La)_(5)(SiO_(4))_(6)F migrate from inside to outside,and finally gather at the outer edge of the phase to form phase Ca_(3)(PO4)2.The RE phase(Ca,Ce,La)_(5)(SiO_(4))_(6)F is distributed inside the furnace-cooled slag,and the RE phase(Ca,Ce,-La,Mg)_(3)(Ti,AI,Nb)_(2)O_(7)is distributed in the surface layer of the furnace-cooled slag.And based on the phase distribution characteristics,the central hollowing method is proposed to realize the preliminary enrichment of valuable elements Ti,Nb and RE in RE slag. | Wanli Yi Xuefeng She Huai Zhang Zhenlong An Jingsong Wang Qingguo Xue | 2023 | Journal of Rare Earths2023，41，5： | 0 |
| 12 | The TFIID pivot of preinitiation complex显示文摘Eukaryotic transcription begins with the assembly of general transcription factors(GTFs)including TFIID,IIA,IIB,IIE,IIF,and IIH,which help Pol II to engage with promoters.The binding of TATA-box binding protein(TBP)to DNA,which causes the bending of TATA sequence,is the first step of the preinitiation complex(PIC)formation,and TBP is essential for almost all gene expression.TBP is an integral part of the TFIID complex which includes 13 other TBP-associated factors(TAFs)and can be separated into 3 submodules(TFIID-A,-B and-C)(Chen et al.,2021a). | Jingdong Xue Wanli Yang Mengyuan Peng Bing Li | 2021 | Science China(Life Sciences)2021，64，12： | 0 |
| 13 | Progressive transductive learning pattern classification via single sphere显示文摘In many machine learning problems,a large amount of data is available but only a few of them can be labeled easily.This provides a research branch to effectively combine unlabeled and labeled data to infer the labels of unlabeled ones,that is,to develop transductive learning.In this article,based on Pattern classification via single sphere(SSPC),which seeks a hypersphere to separate data with the maximum separation ratio,a progressive transductive pattern classification method via single sphere(PTSSPC)is proposed to construct the classifier using both the labeled and unlabeled data.PTSSPC utilize the additional information of the unlabeled samples and obtain better classification performance than SSPC when insufficient labeled data information is available.Experiment results show the algorithm can yields better performance. | Xue Zhenxia Liu Sanyang Liu Wanli | 2009 | Journal of Systems Engineering and Electronics2009，20，3： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员