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| 1 | Characterization of microRNAs in serum: a novel class of biomarkers for diagnosis of cancer and other diseases显示文摘在各种各样的纸巾的 microRNAs (miRNAs ) 的 Dysregulated 表示与许多疾病被联系了,包括癌症。这里,我们证明 miRNAs 在象老鼠,老鼠,牛的胎儿,小牛,和马那样的人和另外的动物的浆液和血浆是在场的。在浆液的 miRNAs 的层次在一样的种类的个人之中稳定、可再现、一致。采用 Solexa,我们定序健康中国题目的所有浆液 miRNAs 并且分别地在男、女的题目发现超过 100 和 91 浆液 miRNAs。我们也为肺癌症, colorectal 癌症,和糖尿病识别了浆液 miRNAs 的特定的表示模式,提供证据那浆液 miRNAs 为各种各样的疾病包含指纹。癌症特定的浆液 miRNAs 由 Solexa 获得了的二个非小的房间肺进一步在 75 个健康施主和 152 个癌症病人的独立审判被验证,用量的反向的抄写聚合酶链反应试金。通过这些分析,我们断定浆液 miRNAs 能为各种各样的癌症和另外的疾病的察觉用作潜在的 biomarkers。 | Xi Chen Yi Ba Lijia Ma Xing Cai Yuan Yin Kehui Wang Jig ang Guo Yujing Zhang Jiangning Chen Xing Guo Qibin Li Xiaoying Li Wenjing Wang Yan Zhang Jin Wang Xueyuan Jiang Yang Xiang Chen Xu Pingping Zheng Juanbin Zhang Ruiqiang Li Hongjie Zhang Xiaobin Shang Ting Gong Guang Ning Jun Wang Ke Zen Junfeng Zhang Chen-Yu Zhang | 2008 | Cell Research2008，18，10： | 975 |
| 2 | Stable classi?cation with limited sample: transferring a 30-m resolution sample set collected in 2015 to mapping 10-m resolution global land cover in 2017显示文摘As the world strives to reduce the impact of population growth, urbanization, agricultural expansion, and climate change on food security, energy and water shortage, resource over-exploration, biodiversity loss, environmental pollution, and ultimately human health, timely and higher resolution land cover information is urgently needed to achieve the sustainable development goals of the United Nations. | Peng Gong Han Liu Meinan Zhang Congcong Li Jie Wang Huabing Huang Nicholas Clinton Luyan Ji Wenyu Li Yuqi Bai Bin Chen Bing Xu Zhiliang Zhu Cui Yuan Hoi Ping Suen Jing Guo Nan Xu Weijia Li Yuanyuan Zhao Jun Yang Chaoqing Yu Xi Wang Haohuan Fu Le Yu Iryna Dronova Fengming Hui Xiao Cheng Xueli Shi Fengjin Xiao Qiufeng Liu Lianchun Song | 2019 | Science Bulletin2019，64，6： | 166 |
| 3 | Relationship between the expression of iNOS,VEGF,tumor angiogenesis and gastric cancer显示文摘AIM:To in vestigate the relationship between the expression of inducible nitric oxide synthase(iNOS),vascular endothelial growth factor(VEGF),the microvascular density(MVD)and the pathological features and clinical staging of gastric cancer.METHODS:Immunohistochemical staining was used for detecting the expression of iNOS and VEGFin46resected specimens of gastric carcinoma;the monoclonal antibody against CD34 was used for displaying vascular endothelial cells,and MVD was detected by counting of CD34-positive vascular endothelial cells.RESULTS:Of 46resected specimens of gastric carcinoma,the rates of expressions of iNOS and VEGF were 58.70%and76.09%,respectively,and MVDaveraged55.59±19.39,Judged by the standard TNM criteria,the rate of expression of iNOS in stageⅣ(84.46%)was higher than those in stageⅠ,Ⅱ,Ⅲ(Fish exact probabilities test,P=0.019,0.023and 0.033,respectively);the rates of expression of VEGFin stage Ⅲ,Ⅳ(76.0%,92.31%,respectively)were higher than those in stageⅠ,Ⅱ(Fis exact probabilities test,P=0.031,0.017,0.022and0.019).MVDs in stageⅢ,Ⅳ(64.72±14.96,67.09±18.29,respectively)were higher than those in stageⅠ,Ⅱ(t\2.378,4.015,2.503and2.450,P<0.05,P<0.001,P<0.001,P<0.05,respectively),In37gastric carcinoma specimens with lymph node metastasis,MVD(68.69±18.07)and the rates of expression of iNOS and VEGF(70.27%,83.78%,respectively)were higher than those in the specimens with absence of metastasis(t=2.205,X^2=6.3587,X^2=6.2584,P<0.01,P<0.05,P<0.05,respectively),MVD and the expressions of iNOS and EGF were not correlated to the location,size or grade of tumor,nor with the depth of invasion of tumor;MVDs in the positive iNOS and VEGF specimens(59.88±18.02,58.39±17.73,repectively)were higher than those in the negative iNOS and VEGF specimens(X^2=6.3587and 6.1574,P<0.05,P<0.05,respectively);thus the expressions of iNOS and VEGF was correlated to MVD,but the expression of iNOS was not correlated to that of VEGF,In addition.of the 46 surviving patients,the 5-year survival rate of patients with positive iNOS or VEGF tumors was significantly less than that of patients with negative iNOS-or VEGF tumors(X^2=4.3842and 5.4073,P<0.05,P<0.05.respectively).CONCLUSION:The expressions of iNOS and VEGF are colosely related to tumor angiogenesis,and are involved in the advancement and the lymph node metastasis;thusMVD and the expressions of iNOS and EGF may serve indexes for evaluating staging of gastric carcinoma and forecasting its risk of metastasis,which will help establish a comprehensive therapeutical measure of post-operative patients and provide a new approach to tumor therapy. | Zheng-Jun Song Ping Gong Yu-E Wu Department of Gastroenterology,First Affiliated Hospital of Xi’an Jiaotong University,Xi’an 710061,ShaanXi Province,China | 2002 | World Journal of Gastroenterology2002，8，4： | 87 |
| 4 | 2018 Chinese Pediatric Cardiology Society(CPCS) guideline for diagnosis and treatment of syncope in children and adolescents显示文摘Syncope belongs to the transient loss of consciousness(TLOC), characterized by a rapid onset, short duration, and spontaneous complete recovery. It is common in children and adolescents, accounting for 1% to 2% of emergency department visits.Recurrent syncope can seriously affect children's physical and mental health, learning ability and quality of life and sometimes cardiac syncope even poses a risk of sudden death. The present guideline for the diagnosis and treatment of syncope in children and adolescents was developed for guiding a better clinical management of pediatric syncope. Based on the globally recent development and the evidence-based data in China, 2018 Chinese Pediatric Cardiology Society(CPCS) guideline for diagnosis and treatment of syncope in children and adolescents was jointly prepared by the Pediatric Cardiology Society, Chinese Pediatric Society, Chinese Medical Association(CMA)/Committee on Pediatric Syncope, Pediatricians Branch, Chinese Medical Doctor Association(CMDA)/Committee on Pediatric Cardiology, Chinese College of Cardiovascular Physicians, Chinese Medical Doctor Association(CMDA)/Pediatric Cardiology Society, Beijing Pediatric Society, Beijing Medical Association(BMA). The present guideline includes the underlying diseases of syncope in children and adolescents, the diagnostic procedures, methodology and clinical significance of standing test and headup tilt test, the clinical diagnosis vasovagal syncope, postural orthostatic tachycardia syndrome, orthostatic hypotension and orthostatic hypertension, and the treatment of syncope as well as follow-up. | Cheng Wang Yaqi Li Ying Liao Hong Tian Min Huang Xiangyu Dong Lin Shi Jinghui Sun Hongfang Jin Junbao Du Jindou An Jie Chen Mingwu Chen Qi Chen Sun Chen Yonghong Chen Zhi Chen Adolphus Kai-tung Chau Junbao Du Zhongdong Du Junkai Duan Hongyu Duan Xiangyu Dong Lin Feng Lijun Fu Fangqi Gong Yonghao Gui Ling Han Zhenhui Han Bing He Zhixu He Xiufen Hu Yimin Hua Guoying Huang Min Huang Ping Huang Yujuan Huang Hongfang Jin Mei Jin Bo Li Fen Li Tao Li Xiaohui Li Xiaoyan Liu Yan Li Haitao Lv Tiewei Lv Zipu Li Luyi Ma Silin Pan Yusheng Pang Hua Peng Yuming Qin Jie Shen Lin Shi Kun Sun Jinghui Sun Hong Tian Jie Tian Cheng Wang Hong Wang Lei Wang Jinju Wang Wendi Wang Yuli Wang Rongzhou Wu Tianhe Xia Yanyan Xiao Chunhong Xie Yanlin Xing Zhenyu Xiong Baoyuan Xu Yi Xu Hui Yan Shiwei Yang Qijian Yi Xia Yu Xianyi Yu Yue Yuan Hongyan Zhang Huili Zhang Li Zhang Qingyou Zhang Xi Zhang Yanmin Zhang Zhiwei Zhang Cuifen Zhao Bin Zhou Hua Zhu | 2018 | Science Bulletin2018，63，23： | 54 |
| 5 | Assessment of duodenogastric reflux by combined continuous intragastric pH and bilirubin monitoring显示文摘AIM: To assess the diagnostic value of a combination of continuous intragastric pH and bilirubin monitoring in the detection of duodenogastric reflux (DGR), and the effects of diet on the bilirubin absorbance.METHODS: 30 healthy volunteers were divided into twogroups: standard diet group (Group 1) 18 cases, free diet group (Group 2) 12 cases. Each subjects were subjected to simultaneous 24 hour intragastric pH and spectrophotometric bilirubin concentration monitoring (Bilitec 2000).RESULTS: There was no difference of preprandial phasebilirubin absorbance between two groups. The absorbanceof postprandial phase was significantly increased in group 2than group 1. There was no difference between preprandialphase and postprandial phase absorbance in group 1.Postprandial phase absorbance was significantly higher ingroup 2. In a comparison of bile reflux with intragastric pHduring night time, there were 4 types of reflux:Simultaneous increase in absorbance and pH in only 19.6%, increase in bilirubin with unchanged pH 33. 3 %, pHincrease with unchanged absorbance 36. 3 %, and bothunchanged in 10. 8 %. Linear regression analysis showed nocorrelation between percertage total time of pH < 4 aridpercentage total time of absortance > 0. 14, r=0.068, P<0.05.CONCLUSION: Because of the dietary effect, highabsorbance fluids or foods should be avoided in detection.Intrsgastric pH and bilirubin monitoring separately predictthe presence of duodenal (and/or pancreatic) reflux and bilereflux. They can not substitute for each other. The detectionof DGR is improved if the two parameters are combinedsimultanoously. | Fei Dai Jun Gong Ru Zhang Jin-Yan Luo You-Ling Zhu Xue-Qin Wang,Department of Gastroenterology,Second Hospital of Xi’ an Jiaotong University,Xi’an 710004,Shaanxi Province,China | 2002 | World Journal of Gastroenterology2002，8，2： | 30 |
| 6 | Minimally invasive percutaneous compression plating versus dynamic hip screw for intertrochanteric fractures: a randomized control trial显示文摘ObjectiveIntertrochanteric 腿节骨折是在老病人的普通损害。动态新潮的螺丝钉(DHS ) 为固定用作标准选择;然而,它有几个缺点。经皮的压缩板(PCCP ) 的研究关于它的功效和安全仍然是不确定的。由比较二个方法,我们与 intertrochanteric 腿节破裂估计了他们 121 个老病人的临床的治疗学的 outcome.MethodsA 总数(类型 AO/OTA 31 .A1-A2 , Evans 类型 1 )随机被划分成经历一个最低限度地侵略的 PCCP 过程或操作持续时间是的一个常规 DHS fixation.ResultsThe 平均数的二个组在 PCCP 组显著地更短( 55.2 ; min 对 88.5 ; min , P ;< ; 0.01 )。血损失是 156.5 ml ± 18.3 在 PCCP 组和 513.2 的 ml; ml ± 66.2 在 DHS 组的 ml (P < 0.01 ) 。在与 PCCP 对待的病人之中, 3.1% 需要输血,与 44.6% 那些那有的 DHS 外科相比(P < 0.01 ) 。PCCP 组显示了不太手术后的复杂并发症(P < 0.05 ) 。在 PCCP 组的麻醉学者分数和哈里斯臀部分数的吝啬的美国社会比在 DHS 组的那些好。在到几个优点的吝啬的医院停留,死亡率,或骨折 healing.ConclusionDue 没有重要差别, PCCP 有潜力为对待 intertrochanteric 破裂成为理想的选择(类型 AO/OTA 31 .A1-A2, Evans 类型 1 ) ,在特别地老。 | Cheng Qiang Huang Wei Gong Xuan Wang Changdong Liang Xi Hu Ning | 2014 | Chinese Journal of Traumatology2014，17，5： | 20 |
| 7 | Alkaloid from Dicranostigma leptopodum(Maxim) Fedde显示文摘Fedde 有的 Dicranostigma leptopodum (格言) 的整个植物的 Phytochemical 调查导致五碱的隔离:dicranostigmine (1 ) , isocorydine (2 ) , corydine (3 ) , protopine (4 ) 和 sinoacutine (5 ) 。Oxomorphine 碱(5 ) 第一次从类 Dicranostigma 被孤立,新复合结构(1 ) 被各种各样的分光镜的方法包括 2D NMR 技术(gCOSY, HMQC, HMBC 和 NOESY ) 和 HRESIMS 阐明。 | Yan Dang Hong Fei Gong Jun Xi Liu Si Jiu Yu | 2009 | Chinese Chemical Letters2009，20，10： | 19 |
| 8 | Memetic computation based on regulation between neural and immune systems: the framework and a case study显示文摘Lamarckian learning has been introduced into evolutionary computation to enhance the ability of local search. The relevant research topic, memetic computation, has received significant amount of interest. In this study, a novel memetic computational framework is proposed by simulating the integrated regulation between neural and immune systems. The Lamarckian learning strategy of simulating the unidirectional regulation of neural system on immune system is designed. Consequently, an immune memetic algorithm based on the Lamarckian learning is proposed for numerical optimization. The proposed algorithm combines the advantages of immune algorithms and mathematical programming, and performs well in both global and local search. The simulation results based on ten low-dimensional and ten high-dimensional benchmark problems show that the immune memetic algorithm outperforms the basic genetic algorithm-based memetic algorithm in solving most of the test problems. | GONG MaoGuo , JIAO LiCheng, LIU Fang & YANG Jie Key Lab of Intelligent Perception and Image Understanding of Ministry of Education of China, Institute of Intelligent Information Processing, Xidian University, Xi’an 710071, China | 2010 | Science China(Information Sciences)2010，53，8： | 16 |
| 9 | Effect of severe acute pancreatitis on pharmacokinetics of Da-Cheng-Qi Decoction components显示文摘AIM:To investigate the effect of severe acute pan- creatitis(SAP)on pharmacokinetics of Da-Cheng-Qi Decoction(DCQD)components in rats. METHODS:Rats were divided into SAP group and sham-operation group as a control group(n=6). Rhein,chrysophanol,rheochrysidin,magnolol,hesperidin and naringin in DCQD were quantified in rat serum by high performance liquid chromatography tandem mass spectrometry for studying their pharmacokinetics. RESULTS:Early absorption of each DCQD component was tended to degrade in SAP group after treatment with DCQD by gavage.The Cmax(chrysophanol,P= 0.0059;rheochrysidin,P=0.0288;magnolol,P= 0.0487;hesperidin,P=0.0277;naringin,P=0.0023) and AUC(rhein,P=0.0186;chrysophanol,P=0.0013; magnolol,P=0.001;hesperidin,P=0.0081;naringin, P=0.0272)of DCQD component were obviously lower in SAP group than in control group.The T1/2α of chrysophanol and rheochrysidin(P=0.0467 and 0.0005,respectively)and Tmax of chrysophanol and rheochrysidin(P=0.0101 and 0.0037,respectively) lasted longer in SAP group than in control group. CONCLUSION:SAP can significantly impact the ab-sorption of DCQD components in rats and their phar-macokinetic parameters. | Han-Lin Gong Wen-Fu Tang Qin Yu Jin Xiang Qing xia Guang-Yuan Chen Xi Huang Mao-Zhi Liang | 2009 | World Journal of Gastroenterology2009，15，47： | 16 |
| 10 | Prevalence, awareness, treatment, and control of hypertension in the non-dialysis chronic kidney disease patients显示文摘 | ZHENG Ying CAI Guang-yan CHEN Xiang-mei FU Ping CHEN Jiang-hua DING Xiao-qiang YU Xue-qing LIN Hong-li LIU Jian XIE Ru-juan WANG Li-ning NI Zhao-hui LIU Fu-you YIN Ai-ping XING Chang-ying WANG Li SHI Wei LIU Jian-she HE Ya-ni DING Guo-hua LI Wen-ge WU Guang-li MIAO Li-ning CHEN Nan SU Zhen MEI Chang-lin ZHAO Jiu-yang GU Yong BAI Yun-kai LUO Hui-min LIN Shan CHEN Meng-hua GONG Li YANG Yi-bin YANG Xiao-ping LI Ying WAN Jian-xin WANG Nian-song LI Hai-ying XI Chun-sheng HAO Li XU Yan FANG Jing-ai LIU Bi-cheng LI Rong-shan WANG Rong ZHANG Jing-hong WANG Jian-qin LOU Tan-qi SHAO Feng-min MEI Feng LIU Zhi-hong YUAN Wei-jie SUN Shi-ren ZHANG Ling ZHOU Chun-hua CHEN Qin-kai JIA Shun-lian GONG Zhi-feng GUAN Guang-ju XIA Tian ZHONG Liang-bao | 2013 | Chinese Medical Journal2013，，12： | 16 |
| 11 | Clinical value of preoperative methylated septin 9 in Chinese colorectal cancer patients显示文摘BACKGROUND The methylated septin 9(mSEPT9) assay was the first blood-based test approved by the United States Food and Drug Administration as a colorectal screening test.However, the diagnostic and prognostic role of preoperative mSEPT9 for colorectal cancer(CRC) in Chinese patients is still unknown.AIM To improve the understanding of diagnostic and prognostic factors, serum m SEPT9 was detected in Chinese CRC patients.METHODS A retrospective analysis of 354 cases, of which 300 had CRC and 54 were normal,was performed in China. Patients' characteristics, treatments, and laboratory data, including age, the date of surgery, Union for International Cancer Control(UICC) stages, distant metastasis(M), and so on, were collected. Methylation levels of SEPT9 were quantified by quantitative, methylation-specific polymerase chain reaction before surgery. In addition, the effects of mSEPT9 on the occurrence and prognosis of 330 CRC cases from The Cancer Genome Atlas(TCGA) database were evaluated using bioinformatics analyses. Potential prognostic factors for overall survival(OS) and progression-free survival(PFS)were evaluated by Kaplan-Meier univariate analysis.RESULTSIn Chinese CRC patients, positive mSEPT9 was strongly associated with advanced UICC stages, deeper invasion by the primary tumor, and more distant metastasis. Methylation levels of SEPT9 were stage-dependent and showed a stepwise increase in UICC stages(I–IV), primary tumor categories(T1–T4),regional node categories(N0–N2), and distant metastasis categories(M0–M1).The patients with positive mSEPT9 showed a tendency toward lower PFS. After analyzing TCGA clinical data, the high mSEPT9 group was found to be obviously correlated only with more distant metastasis. The patients with high mSEPT9 levels showed a tendency toward lower OS. Besides, nine meaningful mSEPT9 sites were found to provide guidance for the follow-up studies.CONCLUSION MSEPT9 analysis may add valuable information to current tumor staging. Serum m SEPT9 in Chinese CRC patients appears to offer promising novel prognostic markers and might be considered for monitoring CRC recurrence. | Xue Yang Zhi-Jie Xu Xi Chen Shuang-Shuang Zeng Long Qian Jie Wei Mei Peng Xiang Wang Wan-Li Liu Hong-Ying Ma Zhi-Cheng Gong Yuan-Liang Yan | 2019 | World Journal of Gastroenterology2019，25，17： | 16 |
| 12 | Decision-Making in Driver-Automation Shared Control:A Review and Perspectives显示文摘Shared control schemes allow a human driver to work with an automated driving agent in driver-vehicle systems while retaining the driver’s abilities to control.The human driver,as an essential agent in the driver-vehicle shared control systems,should be precisely modeled regarding their cognitive processes,control strategies,and decision-making processes.The interactive strategy design between drivers and automated driving agents brings an excellent challenge for human-centric driver assistance systems due to the inherent characteristics of humans.Many open-ended questions arise,such as what proper role of human drivers should act in a shared control scheme?How to make an intelligent decision capable of balancing the benefits of agents in shared control systems?Due to the advent of these attentions and questions,it is desirable to present a survey on the decision making between human drivers and highly automated vehicles,to understand their architectures,human driver modeling,and interaction strategies under the driver-vehicle shared schemes.Finally,we give a further discussion on the key future challenges and opportunities.They are likely to shape new potential research directions. | Wenshuo Wang Xiaoxiang Na Dongpu Cao Jianwei Gong Junqiang Xi Yang Xing Fei-Yue Wang | 2020 | IEEE/CAA Journal of Automatica Sinica2020，7，5： | 14 |
| 13 | Evaluation on the accuracy of digital elevation models显示文摘There is a growing interest in investigating the accuracy of digital elevation model (DEM). However people usually have an unbalanced view on DEM errors. They emphasize DEM sampling errors, but ignore the impact of DEM resolution and terrain roughness on the accuracy of terrain representation. This research puts forward the concept of DEM terrain representation error (Et) and then investigates the generation, factors, measurement and simulation of DEM terrain representation errors. A multi-resolution and multi-relief comparative approach is used as the major methodology in this research. The experiment reveals a quantitative relationship between the error and the variation of resolution and terrain roughness at a global level. Root mean square error (RMS Et) is regressed against surface profile curvature (V) and DEM resolution (R) at 10 resolution levels. It is found that the RMS Et may be expressed as RMS Et = (0.0061 ( V+ 0.0052) ( R - 0.022 ( V + 0.2415. This result may be very useful in forecasting DEM accuracy, as well as in determining the DEM resolution related to the accuracy requirement of particular application. | TANG Guo-an~1, Josef Strobl~2, GONG Jian-ya~3, ZHAO Mu-dan~1, CHEN Zhen-jiang~1 (1. Department of Geography, Northwest University, Xi’an 710069, China 2. Department of Geography and Geoinformation, Salzburg University, Hellbrunnerstr. 34, A-5020 Salzb | 2001 | Journal of Geographical Sciences2001，11，2： | 13 |
| 14 | Spatial and temporal change patterns of net primary productivity and its response to climate change in the Qinghai–Tibet Plateau of China from 2000 to 2015显示文摘The vegetation ecosystem of the Qinghai–Tibet Plateau in China,considered to be the′′natural laboratory′′of climate change in the world,has undergone profound changes under the stress of global change.Herein,we analyzed and discussed the spatial-temporal change patterns and the driving mechanisms of net primary productivity(NPP)in the Qinghai–Tibet Plateau from 2000 to 2015 based on the gravity center and correlation coefficient models.Subsequently,we quantitatively distinguished the relative effects of climate change(such as precipitation,temperature and evapotranspiration)and human activities(such as grazing and ecological construction)on the NPP changes using scenario analysis and Miami model based on the MOD17A3 and meteorological data.The average annual NPP in the Qinghai–Tibet Plateau showed a decreasing trend from the southeast to the northwest during 2000–2015.With respect to the inter-annual changes,the average annual NPP exhibited a fluctuating upward trend from 2000 to 2015,with a steep increase observed in 2005 and a high fluctuation observed from 2005 to 2015.In the Qinghai–Tibet Plateau,the regions with the increase in NPP(change rate higher than 10%)were mainly concentrated in the Three-River Source Region,the northern Hengduan Mountains,the middle and lower reaches of the Yarlung Zangbo River,and the eastern parts of the North Tibet Plateau,whereas the regions with the decrease in NPP(change rate lower than–10%)were mainly concentrated in the upper reaches of the Yarlung Zangbo River and the Ali Plateau.The gravity center of NPP in the Qinghai–Tibet Plateau has moved southwestward during 2000–2015,indicating that the increment and growth rate of NPP in the southwestern part is greater than those of NPP in the northeastern part.Further,a significant correlation was observed between NPP and climate factors in the Qinghai–Tibet Plateau.The regions exhibiting a significant correlation between NPP and precipitation were mainly located in the central and eastern Qinghai–Tibet Plateau,and the regions exhibiting a significant correlation between NPP and temperature were mainly located in the southern and eastern Qinghai–Tibet Plateau.Furthermore,the relative effects of climate change and human activities on the NPP changes in the Qinghai–Tibet Plateau exhibited significant spatial differences in three types of zones,i.e.,the climate change-dominant zone,the human activity-dominant zone,and the climate change and human activity interaction zone.These research results can provide theoretical and methodological supports to reveal the driving mechanisms of the regional ecosystems to the global change in the Qinghai–Tibet Plateau. | GUO Bing ZANG Wenqian YANG Fei HAN Baomin CHEN Shuting LIU Yue YANG Xiao HE Tianli CHEN Xi LIU Chunting GONG Rui | 2020 | Journal of Arid Land2020，12，1： | 12 |
| 15 | Anti-HBV effect of TAT-HBV targeted ribonuclease显示文摘AIM: To prepare and purify TAT-HBV targeted ribonuclease fusion protein, evaluate its transduction activity and investigate its effect on HBV replication in 2.2.15 cells.METHODS: The prokaryotic expression vector pTAT containing TR gene was used in transforming E. coli BL21(DE3) LysS and TR was expressed with the induction of IPTG. The TAT-TR fusion protein was purified using Ni-NTA-agrose and PD-10 desalting columns, and analyzed by SDSPAGE. Transduction efficiency of TAT-TR was detected with immunofluorescence assay and the concentration of HBeAg in the supernatant of the 2.2.15 cells was determined via solid-phase radioimmunoassay (spRIA). MTT assay was used to detect the cytotoxicity of TAT-TR.RESULTS: The SDS-PAGE showed that the TAT-TR fusion protein was purified successfully, and the purity of TAT-TR was 90 %. The visualization of TAT-TR by immunofiuorescence assay indicated its high efficiency in transducing 2.2.15 cells.RIA result suggests that TAT-TR could inhibit the replication of HBV effectively, it didn′t affect cell growth and had no cytotoxicity.CONCLUSION: TAT-TR possesses a significant anti-HBV activity and the preparation of TAT-TR fusion protein has laid the foundation for the use of TR in the therapeutic trial of HBV infection. | Jin Ding Jun Liu Cai-Fang Xue Wei-Dong Gong Ying-Hui Li Ya Zhao Department of Etiology,Fourth Military Medical University,Xi’an 710032,Shaanxi Province,China | 2003 | World Journal of Gastroenterology2003，9，7： | 12 |
| 16 | Second-look arthroscopic evaluation of the articular cartilage after primary single-bundle and double-bundle anterior cruciate ligament reconstructions显示文摘在前面的十字形的系带( ACL ) reconstruction.No 报告关于比较变化在以后的软骨被出版了以后,背景几报告显示出关节的软骨退化的前进单个捆( SB )并且这研究的双捆( DB ) ACL reconstructions.The 目的是在 SB 和 DB ACL 以后评估关节的软骨变化由收到了 arthroscopic ACL 重建的 99 个病人回顾地是的秒看 arthroscopy.Methods | WANG Hai-jun AO Ying-fang CHEN Lian-xu GONG Xi WANG Yong-jian MA Yong LEUNG Kevin Kar Ming YU Jia-kuo | 2011 | Chinese Medical Journal2011，，21： | 12 |
| 17 | Dynamic resistance exercise increases skeletal muscle-derived FSTL1 inducing cardiac angiogenesis via DIP2A-Smad2/3 in rats following myocardial infarction显示文摘Purpose:The aim of this study was to investigate the potential of dynamic resistance exercise to generate skeletal muscle-derived follistatin like-1(FSTL1),which may induce cardioprotection in rats following myocardial infarction(MI)by inducing angiogenesis.Methods:Male,adult Sprague-Dawley rats were randomly divided into 5 groups(n=12 in each group):sham group(S),sedentary MI group(MI),MI+resistance exercise group(MR),MI+adeno-associated virus(AAV)-FSTL1 injection group(MA),and MI+AAV-FSTL1 injection+resistance exercise group(MAR).The AAV-FSTL1 vector was prepared by molecular biology methods and injected into the anterior tibialis muscle.The MI model was established by ligation of the left anterior descending coronary artery.Rats in the MR and MAR groups underwent 4 weeks of dynamic resistance exercise training using a weighted climbing-up ladder.Heart function was evaluated by hemodynamic measures.Collagen volume fraction of myocardium was observed and analyzed by Masson’s staining.Human umbilical vein vessel endothelial cells culture and recombinant human FSTL1 protein or transforming growth factor-b receptor 1(TGFbR1)inhibitor treatment were used to elucidate the molecular signaling mechanism of FSTL1.Angiogenesis,cell proliferation,and disco interacting protein 2 homolog A(DIP2A)location were observed by immunofluorescence staining.The expression of FSTL1,DIP2A,and the activation of signaling pathways were detected by Western blotting.Angiogenesis of endothelial cells was observed by tubule experiment.One-way analysis of variance and Student’s t test were used for statistical analysis.Results:Resistance exercise stimulated the secretion of skeletal muscle FSTL1,which promoted myocardial angiogenesis,inhibited pathological remodeling,and protected cardiac function in MI rats.Exercise facilitated skeletal muscle FSTL1 to play a role in protecting the heart.Exogenous FSTL1 promoted the human umbilical vein vessel endothelial cells proliferation and up-regulated the expression of DIP2A,while TGFbR1 inhibitor intervention down-regulated the phosphorylation level of Smad2/3 and the expression of vascular endothelial growth factor-A,which was not conducive to angiogenesis.FSTL1 bound to the receptor,DIP2A,to regulate angiogenesis mainly through the Smad2/3 signaling pathway.FSTL1-DIP2A directly activated Smad2/3 and was not affected by TGFbR1.Conclusion:Dynamic resistance exercise stimulates the expression of skeletal muscle-derived FSTL1,which could supplement the insufficiency of cardiac FSTL1 and promote cardiac rehabilitation through the DIP2A-Smad2/3 signaling pathway in MI rats. | Yue Xi Meili Hao Qiaoqin Liang Yongxia Li Da-Wei Gong Zhenjun Tian | 2021 | Journal of Sport and Health Science2021，10，5： | 11 |
| 18 | 军队战时饮用水卫生标准显示文摘军队战时饮用水卫生标准(GJB651-89)规定了军队战时饮用的水质标准,及对水源选择、卫生防护、水质检验的要求。适用于军队战时饮水期为7天(军用毒剂染毒时3天以内)或90天以内各种给水方式的饮用水水质要求;也适用于军队平时行军、野营及其它野外条件的饮用水的水质要求。水质标准对27项指标规定了限量值,是按60kg体重的健康成人,每人每天饮水量为5L,饮水期限为7天或9o天而制定的。 | Hou Yue Jiang Xingjing Zhuo Jianbo Shi Peicai Gong Shuming(Department of Military Hygiene,The Fourth Military Medical University,Xi’an) | 1994 | 解放军预防医学杂志1994，12，2： | 10 |
| 19 | Association between well-characterized lung cancer IncRNA polymorphisms and platinum-based chemotherapy toxicity in Chinese patients with lung cancer显示文摘基于铂的化疗是为大多数肺癌症病人的标准首要的治疗。然而,基于铂的化疗极大地导致的毒性阻碍它的临床的使用。以前的研究证明有在长度的超过 200 核苷酸的长非编码的 RNA (lncRNAs ) 影响药反应和毒性。在现在的学习,我们与肺癌症在中国病人与基于铂的化疗毒性调查了描绘得好的肺癌症 lncRNA 多型性的协会。467 个肺癌症病人的一个总数与基于铂的化疗对待因为至少二个周期被招募。我们首先集中了于胃肠并且 hematological 毒性。在 8 lncRNAs (HOTTIP, HOTAIT, H19, ANRIL, CCAT2, MALAT1, MEG3,和 POLR2E ) 以内的 14 潜在地功能的多型性的一个总数是 genotyped。无条件的后勤的回归分析被进行估计协会。基因基因和基因环境相互作用用软件被识别概括 multifactor 维数减小(GMDR ) 。ANRIL rs1333049 在一个添加剂模型与严重全面毒性被联系(调整 OR=0.723, 95% CI=0.541-0.965, P=0.028 ) 。ANRIL rs1333049 也在两个与严重胃肠的毒性被联系添加剂(调整 OR=0.690, 95% CI=0.489-0.974, P=0.035 ) 并且主导(调整 OR=0.558, 95% CI=0.335-0.931, P=0.025 ) 模型。MEG3 rs116907618 在一个添加剂模型与严重胃肠的毒性被联系(调整 OR=1.717, 95% CI=1.007-2.927, P=0.047 ) 。GMDR 为 hematological 毒性作为最好的预兆的模型识别了 POLR2E rs3787016-HOTTIP rs3807598 化疗政体的三因素的相互作用模型。在结论, ANRIL 和 MEG3,基因多型性与严重的铂毒性被联系并且能与肺癌症在中国病人为预告的处理评估被看作 biomarkers。 | Wei-jing GONG Jing-bo PENG Ji-ye YIN Xiang-ping LI Wei ZHENG Ling XIAO Li-ming TAN Di XIAO Yi-xin CHEN Xi LI Hong-hao ZHOU Zhao-qian LIU | 2017 | Acta Pharmacologica Sinica2017，38，4： | 10 |
| 20 | Strategies to improve aerobic granular sludge stability and nitrogen removal based on feeding mode and substrate显示文摘A systemic strategy was proposed to improve aerobic granular sludge(AGS)stability and nitrogen(N)removal efficiency by optimizing feeding mode and substrate aiming at complicated wastewater characteristics.Key functional groups at the genus level identified by high-throughput sequencing were evaluated as well.The results showed that anaerobic feeding mode and acetate promoted the compact AGS formation with excellent total nitrogen(TN)removal efficiency(averaging 91.7%±4.1%)at various dissolved oxygen conditions.While the aerobic feeding mode led to a loose AGS structure with a vulnerable anaerobic core and poor TN removal efficiency(averaging58.8%±7.4%).Simultaneous nitrification and denitrification process played the dominant role in N removal in compact AGS over the alternating nitrification and denitrification process.High-concentration glucose undermined feast-famine condition with filamentous bacteria growth out of granule and decreased TN removal efficiency to 67.3%±15.2%.Lower food to microorganism ratio may result in a lower N removal rate attributed to the sharply increased biomass concentration fed by glucose.Ammonia-oxidizing bacteria,nitrite-oxidizing bacteria,denitrifying bacteria,and denitrifying phosphorus accumulation organisms enriched during AGS granulation also contributed to the efficient N removal.The proposed strategy provided insights into the relationship between various factors and stable AGS formation,and systemic operation methods for various complicated wastewater treatment. | Quan Yuan Hui Gong Hao Xi Heng Xu Zhengyu Jin Nasir Ali Kaijun Wang | 2019 | Journal of Environmental Sciences2019，31，10： | 10 |
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