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| 1 | Identification of a novel coronavirus causing severe pneumonia in human:a descriptive study显示文摘Background:Human infections with zoonotic coronaviruses(CoVs),including severe acute respiratory syndrome(SARS)-CoV and Middle East respiratory syndrome(MERS)-CoV,have raised great public health concern globally.Here,we report a novel batorigin CoV causing severe and fatal pneumonia in humans.Methods:We collected clinical data and bronchoalveolar lavage(BAL)specimens from five patients with severe pneumonia from Wuhan Jinyintan Hospital,Hubei province,China.Nucleic acids of the BAL were extracted and subjected to next-generation sequencing.Virus isolation was carried out,and maximum-likelihood phylogenetic trees were constructed.Results:Five patients hospitalized from December 18 to December 29,2019 presented with fever,cough,and dyspnea accompanied by complications of acute respiratory distress syndrome.Chest radiography revealed diffuse opacities and consolidation.One of these patients died.Sequence results revealed the presence of a previously unknownβ-CoV strain in all five patients,with 99.8%to 99.9%nucleotide identities among the isolates.These isolates showed 79.0%nucleotide identity with the sequence of SARS-CoV(GenBank NC_004718)and 51.8%identity with the sequence of MERS-CoV(GenBank NC_019843).The virus is phylogenetically closest to a bat SARS-like CoV(SL-ZC45,GenBank MG772933)with 87.6%to 87.7%nucleotide identity,but is in a separate clade.Moreover,these viruses have a single intact open reading frame gene 8,as a further indicator of bat-origin CoVs.However,the amino acid sequence of the tentative receptor-binding domain resembles that of SARS-CoV,indicating that these viruses might use the same receptor.Conclusion:A novel bat-borne CoV was identified that is associated with severe and fatal respiratory disease in humans. | Li-Li Ren Ye-Ming Wang Zhi-Qiang Wu Zi-Chun Xiang Li Guo Teng Xu Yong-Zhong Jiang Yan Xiong Yong-Jun Li Xing-Wang Li Hui Li Guo-Hui Fan Xiao-Ying Gu Yan Xiao Hong Gao Jiu-Yang Xu Fan Yang Xin-Ming Wang Chao Wu Lan Chen Yi-Wei Liu Bo Liu Jian Yang Xiao-Rui Wang Jie Dong Li Li Chao-Lin Huang Jian-Ping Zhao Yi Hu Zhen-Shun Cheng Un-Lin Liu Zhao-Hui Qian Chuan Qin Qi Jin Bin Cao Jian-Wei Wang | 2020 | Chinese Medical Journal2020，，9： | 99 |
| 2 | Effects of different resuscitation fluid on severe acute pancreatitis显示文摘AIM: To compare effects of different resuscitation fluid on microcirculation, inflammation, intestinal barrier and clinical results in severe acute pancreatitis (SAP). METHODS: One hundred and twenty patients with SAP were enrolled at the Pancreatic Disease Institute between January 2007 and March 2010. The patients were randomly treated with normal saline (NS group), combination of normal saline and hydroxyethyl starch (HES) (SH group), combination of normal saline, hydroxyethyl starch and glutamine (SHG group) in resuscitation. The ratio of normal saline to HES in the SH and SHG groups was 3:1. The glutamine (20% glutamine dipeptide, 100 mL/d) was supplemented into the resuscitation liquid in the SHG group. Complications and outcomes including respiratory and abdominal infection, sepsis, abdominal hemorrhage, intra-abdominal hypertension, abdominal compartment syndrome (ACS), renal failure, acute respiratory distress syndrome (ARDS), multiple organ dysfunction syndrome (MODS), operation intervention, length of intensive care unit stay, length of hospital stay, and mortality at 60 d were compared. Moreover, blood oxygen saturation (SpO 2 ), gastric intramucosal pH value (pHi), intra-abdominal pressure (IAP), inflammation cytokines, urine lactulose/mannitol (L/M) ratio, and serum endotoxin were investigated to evaluate the inflammatory reaction and gut barrier. RESULTS: Compared to the NS group, patients in the SH and SHG groups accessed the endpoint more quickly (3.9 ± 0.23 d and 4.1 ± 0.21 d vs 5.8 ± 0.25 d, P < 0.05) with less fluid volume (67.26 ± 28.53 mL/kg/d, 61.79 ± 27.61 mL/kg per day vs 85.23 ± 21.27 mL/kg per day, P < 0.05). Compared to the NS group, incidence of renal dysfunction, ARDS, MODS and ACS in the SH and SHG groups was obviously lower. Furthermore, incidence of respiratory and abdominal infection was significantly decreased in the SH and SHG groups, while no significant difference in sepsis was seen. Moreover, less operation time was needed in the SH and SHG group than the NS group, but the difference was not significant. The mortality did not differ significantly among these groups. Blood SpO 2 and gastric mucosal pHi in the SH and SHG groups increased more quickly than in the NS group, while IAP was significantly decreased in the SH and SHG group. Moreover, the serum tumor necrosis factor-α, interleukin-8 and C-reactive protein levels in the SH and SHG groups were obviously lower than in the NS group at each time point. Furthermore, urine L/M ratio and serum endotoxin were significantly lower in the SH group and further decreased in the SHG group.CONCLUSION: Results indicated that combination of normal saline, HES and glutamine are more efficient in resuscitation of SAP by relieving inflammation and sustaining the intestinal barrier. | Gang Zhao Jun-Gang Zhang He-Shui Wu Jin Tao Qi Qin Shi-Chang Deng Yang Liu Lin Liu Bo Wang Kui Tian Xiang Li Shuai Zhu Chun-You Wang | 2013 | World Journal of Gastroenterology2013，19，13： | 56 |
| 3 | Erianin,a novel dibenzyl compound in Dendrobium extract,inhibits lung cancer cell growth and migration via calcium/calmodulin-dependent ferroptosis显示文摘Ferroptosis,a novel form of programmed cell death,is characterized by iron-dependent lipid peroxidation and has been shown to be involved in multiple diseases,including cancer.Stimulating ferroptosis in cancer cells may be a potential strategy for cancer therapy.Therefore,ferroptosis-inducing drugs are attracting more attention for cancer treatment.Here,we showed that erianin,a natural product isolated from Dendrobium chrysotoxum Lindl,exerted its anticancer activity by inducing cell death and inhibiting cell migration in lung cancer cells.Subsequently,we demonstrated for the first time that erianin induced ferroptotic cell death in lung cancer cells,which was accompanied by ROS accumulation,lipid peroxidation,and GSH depletion.The ferroptosis inhibitors Fer-1 and Lip-1 but not Z-VAD-FMK,CQ,or necrostatin-1 rescued erianin-induced cell death,indicating that ferroptosis contributed to erianin-induced cell death.Furthermore,we demonstrated that Ca^(2+)/CaM signaling was a critical mediator of erianin-induced ferroptosis and that blockade of this signaling significantly rescued cell death induced by erianin treatment by suppressing ferroptosis.Taken together,our data suggest that the natural product erianin exerts its anticancer effects by inducing Ca^(2+)/CaMdependent ferroptosis and inhibiting cell migration,and erianin will hopefully serve as a prospective compound for lung cancer treatment. | Peng Chen Qibiao Wu Jiao Feng Lili Yan Yitian Sun Shuiping Liu Yu Xiang Mingming Zhang Ting Pan Xiaying Chen Ting Duan Lijuan Zhai Bingtao Zhai Wengang Wang Ruonan Zhang Bi Chen Xuemeng Han Yicong Li Liuxi Chen Ying Liu Xingxing Huang Ting Jin Wenzheng Zhang Hong Luo Xiaohui Chen Yongqiang Li Qiujie Li Guohua Li Qin Zhang Lvjia Zhuo Zuyi Yang Huifen Tang Tian Xie Xiaoping Ouyang Xinbing Sui | 2020 | Signal Transduction and Targeted Therapy2020，5，1： | 42 |
| 4 | Effect of hepatocyte apoptosis induced by TNF-α on acute severe hepatitis in mouse models显示文摘AIM To study the effect of hepatocyteapoptosis and necrosis induced by TNF-α on thepathogenesis of acute severe hepatitis(ASH).METHODS The model of ASH was prepared inD-galactosamine(GAIN)sensitized BALB/c miceby injection of either endotoxin(ET)or tumornecrosis factor-α(TNF-α).Morphologicalchanges of apoptotic hepatocytes were studiedby both light and electron microscope and in siteend labeling method(ISEL).Molecular biologicalchanges of DNA ladder were observed byelectrophoresis of extract from liver tissues.Biochemical changes were measured by alanineaminotransferase(ALT),asparticaminotransferase(AST)and TNF-α.The relationbetween apoptosis and necrosis was evaluatedsimultaneously.RESULTS The sequence of hepatocyteapoptosis,necrosis,and final death from ASHwas observed both in GAIN/ET and GAIN/TNF-agroup.Apoptosis was prominent at 3.5 h and 5 hafter injection of inducer,while necrosis becamedominant at 9 h after challenge.The appearanceof apoptosis was earlier in GAIN/TNF-α groupthan that in GAIN/ ET group.Pretreatment ofmice with antiTNF IgG1 may completely preventthe liver injury induced by GalN/ET.CONCLUSION TNF-α can cause liver damageby inducing hepatic apoptosis and necrosis inmice with endotoxemia. | Guo Qing Zang Xia Qiu Zhou Hong Yu Qing Xie Guo Ming Zhao Bin Wang Qing Guo Yue Qin Xiang Dan Liao Department of Infectious Diseases,Rujin Hospital,Shanghai Second Medical University,Shanghai 200025,China | 2000 | World Journal of Gastroenterology2000，6，5： | 30 |
| 5 | Role of oxidative stress in the apoptosis of hepatocellular carcinoma induced by combination of arsenic trioxide and ascorbic acid显示文摘瞄准:现在的学习被设计决定位于三氧化二砷的联合使用导致的 apoptosis 的改进下面的可能的小径(作为(2 ) O (3 )) 并且抗坏血酸(AA ) 。方法:细胞内部的反应的氧种类(ROS ) 的水平与上载的一根氧化敏感的荧光灯的探针(6-carboxy-2',7' dichlorodihydrofluorescein 乙酰乙酸盐) 借助于流动血细胞计数分析被检测。谷胱甘肽(GSH ) 的活动,谷胱甘肽过氧化物酶(GPx ) ,和超级氧化物歧化酶(草皮) 被生物化学的方法检测。mitochondrial 膜电位被流动血细胞计数分析与玫瑰精 123 测量染色。caspase-3 的 Bcl-2, Bax,和 p17 子单元用西方的污点方法被分析。apoptosis 率被流动血细胞计数与 annexin-V/propidium 碘化物染色决定。结果:与相比当( 2 ) O ( 3 )( 2.0 micromol/L )独自对待,( 2 )在有 AA ( 100 micromol/L )的联合的 O ( 3 )( 2.0 micromol/L )减少了从 101.30+/-5.76 的细胞内部的 GSH 内容到 81.91+/-3.12 mg/g 蛋白质,并且增加了 ROS 从 127.61+/-5.12 铺平到 152.60+/-5.88 ,它被代表由 2 , 7-dichlorofluorescein 紧张。线粒体膜电位的损失从 1269.97+/-36.11 被增加到 1540.52+/-52.63,它被荧光紧张介绍。caspase-3 表示的 p17 子单元被增加近似 2 褶层。然而,草皮和 GPx 弄空和到 Bax 的 Bcl-2 的比率等于独自对待的 As2O3 (P>0.05 ) 的。什么时候 ROS 清道工人, N-acetyl-L-cysteine,被加到当(2 ) O (3 ) 和 AA 联合了处理组, apoptosis 率从 15.60 %+/-1.14% 减少了到 9.48%+/-0.67% ,和 ROS 水平从 152.60+/-5.88 减少了到 102.77+/-10.25。结论:当(2 ) O (3 ) 由增加 ROS 水平通过氧化小径导致了 apoptosis, AA 加强了。这可以是弄空的结果细胞内部的 GSH。它可以影响下游的串联追随者 ROS,包括线粒体去极和 caspase-3 激活。然而,草皮和 GPx 弄空和到 Bax 的 Bcl-2 的比率影响了由因为(2 ) O (3 ) 是,别发现被 AA 加强。 | Jing-jing LI Qiang TANG Yuan LI Ben-rong HU Zhang-yin MING Qin FU Jia-qing QIAN Ji-zhou XIANG | 2006 | Acta Pharmacologica Sinica2006，27，8： | 25 |
| 6 | Roles of nitric oxide in protective effect of berberine in ethanol-induced gastric ulcer mice显示文摘瞄准:在老鼠在导致乙醇的胃溃疡上调查小檗硷的保护的效果。方法:胃溃疡被乙醇的口头的摄取导致。氮的氧化物(没有) 内容被测量,并且内皮的氮的氧化物 synthase (eNOS ) 的 mRNA 表示和可诱导的氮的氧化物 synthase (i NOS ) 被反向的抄写聚合酶链反应(RT-PCR ) 分析。结果:在在乙醇的口服以后的 1 h, 2 h, 3 h 和 6 h 的溃疡索引(UI ) 分别地是 23.8+/-1.4, 23.3+/-2.2, 22.3+/-1.2 和 20.8+/-1.1。在对待 berberine 的组(5 mg/kg 和 50 mg/kg ) 的 UI 是不到控制组。内容不在控制组是在胃的果汁和 5.8+/-1.1 micromol/g 的 73.3+/-7.3 microL/L , 94.0+/-9.2 microL/L , 109.6+/-6.4 microL/L 和 138.2+/-10.2 microL/L 在在 1 h , 2 h , 3 h 和 6 h 的胃的织物的蛋白质, 8.3+/-1.1 micromol/g 蛋白质, 9.8+/-1.1 micromol/g 蛋白质和 11.9+/-1.2 micromol/g 蛋白质,分别地,在乙醇的口服以后。内容不在对待 berberine 的组(5 mg/kg 和 50 mg/kg ) 比在在乙醇的口服以后的 1 h | Long-rui PAN Qiang TANG Qin FU Ben-rong HU Ji-zhou XIANG Jia-qing QIAN | 2005 | Acta Pharmacologica Sinica2005，26，11： | 23 |
| 7 | GSDMB promotes non-canonical pyroptosis by enhancing caspase-4 activity显示文摘Gasdermin B (GSDMB) has been reported to be associated with immune diseases in humans, but the detailed molecular mechanisms remain unsolved. The N-terminus of GSDMB by itself, unlike other gasdermin family proteins, does not induce cell death. Here, we show that GSDMB is highly expressed in the leukocytes of septic shock patients, which is associated with increased release of the gasdermin D (GSDMD) N-terminus. GSDMB expression and the accumulation of the N-terminal fragment of GSDMD are induced by the activation of the non-canonical pyroptosis pathway in a human monocyte cell line. The downregulation of GSDMB alleviates the cleavage of GSDMD and cell death. Consistently, the overexpression of GSDMB promotes GSDMD cleavage, accompanied by increased LDH release. We further found that GSDMB promotes caspase-4 activity, which is required for the cleavage of GSDMD in non-canonical pyroptosis, by directly binding to the CARD domain of caspase-4. Our study reveals a GSDMB-mediated novel regulatory mechanism for non-canonical pyroptosis and suggests a potential new strategy for the treatment of inflammatory diseases. | Qin Chen Peiliang Shi Yufang Wang Dayuan Zou Xiuwen Wu Dingyu Wang Qiongyuan Hu Yujie Zou Zan Huang Jianan Ren Zhaoyu Lin Xiang Gao | 2019 | Journal of Molecular Cell Biology2019，11，6： | 22 |
| 8 | Mitochondrial ROS promote macrophage pyroptosis by inducing GSDMD oxidation显示文摘Disrupted mitochondrial membrane potential (MMP) and reactive oxygen species (ROS) generation are often associated with macrophage pyroptosis. It remains unclear how these forms of mitochondrial dysfunction relate to inflammasome activation and gasdermin-D (Gsdmd) cleavage, two central steps of the pyroptotic process. Here, we also found MMP collapse and ROS generation induced by Nlrp3 inflammasome activation as previous studies reported. The elimination of ROS alleviated the cleavage of Gsdmd, suggesting that Gsdmd cleavage occurs downstream of ROS release. Consistent with this result, hydrogen peroxide treatment augmented the cleavage of Gsdmd by caspase-1. Indeed, four amino acid residues of Gsdmd were oxidized under oxidative stress in macrophages. The efficiency of Gsdmd cleavage by inflammatory caspase-1 was dramatically reduced when oxidative modification was blocked by mutation of these amino acid residues. These results demonstrate that Gsdmd oxidation serves as a de novo mechanism by which mitochondrial ROS promote Nlrp3 inflammasome-dependent pyroptotic cell death. | Yufang Wang Peiliang Shi Qin Chen Zan Huang Dayuan Zou Jingzi Zhang Xiang Gao Zhaoyu Lin | 2019 | Journal of Molecular Cell Biology2019，11，12： | 23 |
| 9 | Age-related rhesus macaque models of COVID-19显示文摘Background:Since December 2019,an outbreak of the Corona Virus disease 2019(COVID-19)caused by severe acute respiratory syndrome coronavirus(SARS-CoV-2)in Wuhan,China,has become a public health emergency of international concern.The high fatality of aged cases caused by SARS-CoV-2 was a need to explore the possible age-related phenomena with non-human primate models.Methods:Three 3-5 years old and two 15 years old rhesus macaques were intratracheally infected with SARS-CoV-2,and then analyzed by clinical signs,viral replication,chest X-ray,histopathological changes and immune response.Results:Viral replication of nasopharyngeal swabs,anal swabs and lung in old monkeys was more active than that in young monkeys for 14 days after SARS-CoV-2 challenge.Monkeys developed typical interstitial pneumonia characterized by thickened alveolar septum accompanied with inflammation and edema,notably,old monkeys exhibited diffuse severe interstitial pneumonia.Viral antigens were detected mainly in alveolar epithelial cells and macrophages.Conclusion:SARS-CoV-2 caused more severe interstitial pneumonia in old monkeys than that in young monkeys.Rhesus macaque models infected with SARS-CoV-2 provided insight into the pathogenic mechanism and facilitated the development of vaccines and therapeutics against SARS-CoV-2 infection. | Pin Yu Feifei Qi Yanfeng Xu Fengdi Li Peipei Liu Jiayi Liu Linlin Bao Wei Deng Hong Gao Zhiguang Xiang Chong Xiao Qi Lv Shuran Gong Jiangning Liu Zhiqi Song Yajin Qu Jing Xue Qiang Wei Mingya Liu Guanpeng Wang Shunyi Wang Haisheng Yu Xing Liu Baoying Huang Wenling Wang Li Zhao Huijuan Wang Fei Ye Weimin Zhou Wei Zhen Jun Han Guizhen Wu Qi Jin Jianwei Wang Wenjie Tan Chuan Qin | 2020 | Animal Models and Experimental Medicine2020，3，1： | 22 |
| 10 | Relationship between tumor necrosis factor-α and liver fibrosis显示文摘RelationshipbetweentumornecrosisfactorαandliverfibrosisWANGXin,CHENYueXiang,XUCaiFu,ZHAOGuoNing,HUANGYuXinandWANGQinLiD... | WANG Xin, CHEN Yue Xiang, XU Cai Fu, ZHAO Guo Ning, HUANG Yu Xin and WANG Qin Li Department of Gastroenterology, Tangdu Hospital, The Fourth Military Medical University, Xi′an 710038, Shaanxi Province, China | 1998 | World Journal of Gastroenterology1998，4，1： | 21 |
| 11 | Human urokinase-type plasminogen activator gene-modifiedbone marrow-derived mesenchymal stem cells attenuateliver fibrosis in rats by down-regulating the Wnt signalingpathway显示文摘AIM: To evaluate the therapeutic effects of bone marrow-derived mesenchymal stem cells(BMSCs) with human urokinase-type plasminogen activator(u PA) on liver fibrosis, and to investigate the mechanism of gene therapy.METHODS: BMSCs transfected with adenovirusmediated human urokinase plasminogen activator(Adu PA) were transplanted into rats with CCl4-induced liver fibrosis. All rats were sacrificed after 8 wk, and their serum and liver tissue were collected for biochemical, histopathologic, and molecular analyzes. The degree of liver fibrosis was assessed by hematoxylin and eosin or Masson's staining. Western blot and quantitative reverse transcription-polymerase chain reaction were used to determine protein and m RNA expression levels.RESULTS: Serum levels of alanine aminotransferase, aminotransferase, total bilirubin, hyaluronic acid, laminin, and procollagen type Ⅲ were markedly decreased, whereas the levels of serum albumin were increased by u PA gene modified BMSCs treatment. Histopathology revealed that chronic CCl4-treatment resulted in significant fibrosis while u PA gene modified BMSCs treatment significantly reversed fibrosis. By quantitatively analysing the fibrosis area of liver tissue using Masson staining in different groups of animals, we found that model animals with CCl4-induced liver fibrosis had the largest fibrotic area(16.69% ± 1.30%), while fibrotic area was significantly decreased by BMSCs treatment(12.38% ± 2.27%) and was further reduced by u PA-BMSCs treatment(8.31% ± 1.21%). Both protein and m RNA expression of β-catenin, Wnt4 and Wnt5 a was down-regulated in liver tissues following u PA gene modified BMSCs treatment when compared with the model animals.CONCLUSION: Transplantation of u PA gene modified BMSCs suppressed liver fibrosis and ameliorated liver function and may be a new approach to treating liver fibrosis. Furthermore, treatment with u PA gene modified BMSCs also resulted in a decrease in expression of molecules of the Wnt signaling pathway. | Zhi-Gang Ma Xiao-Dan Lv Ling-Ling Zhan Lan Chen Qi-Yuan Zou Ji-Qiao Xiang Jiao-Li Qin Wei-Wei Zhang Zhao-Jing Zeng Hui Jin Hai-Xing Jiang Xiao-Ping Lv | 2016 | World Journal of Gastroenterology2016，22，6： | 21 |
| 12 | Regulatory Peptides Modulate Adhesion of Polymorphonuclear Leukocytes to Bronchial Epithelial Cells through Regulation of Interleukins, ICAM-1 and NF-κB/IκB显示文摘 | Jian-Song ZHANG Yu-Rong TAN Yang XIANG Zi-Qiang LUO Xiao-Qun QIN | 2006 | Acta Biochimica et Biophysica Sinica2006，38，2： | 18 |
| 13 | Clinical value of fecal calprotectin in determining disease activity of ulcerative colitis显示文摘AIM: To investigate possibility and clinical application of fecal calprotectin in determining disease activity of ulcerative colitis (UC).METHODS: The enzyme-linked immunosorbent assay (ELISA) was used to measure the concentrations of calprotectin in feces obtained from 66 patients with UC and 20 controls. C-reactive protein (CRP), erythrocyte sedimentation rate (ESR), acid glycoprotein (AGP) were also measured and were compared with calprotectin in determining disease activity of UC. The disease activity of UC was also determined by the Sutherland criteria.RESULTS: The fecal calprotectin concentration in the patients with active UC was significantly higher than that in the inactive UC and in the controls (402.16 ± 48.0 μg/g vs 35.93 ± 3.39 μg/g, 11.5 ± 3.42 μg/g, P < 0.01). The fecal calprotectin concentration in the inactive UC group was significantly higher than that in the control group (P < 0.05). A significant difference was also found in the patients with active UC of mild, moderate and severe degrees. The area under the curve of the receiver operating characteristics (AUCROC) was 0.975, 0.740, 0.692 and 0.737 for fecal calprotectin, CRP, ESR and AGP, respectively. There was a strong correlation between the fecal calprotectin concentration and the endoscopic gradings for UC (r = 0.866, P < 0.001).CONCLUSION: Calprotectin in the patient's feces can reflect the disease activity of UC and can be used as a rational fecal marker for intestinal inflammation in clinical practice. This kind of marker is relatively precise, simple and noninvasive when compared with other commonly-used markers such as CRP, ESR and AGP. | Jun-Ying Xiang Qin Ouyang Guo-Dong Li Nan-Ping Xiao | 2008 | World Journal of Gastroenterology2008，14，1： | 16 |
| 14 | COVID-19 Outbreak Caused by Contaminated Packaging of Imported Cold-Chain Products—Liaoning Province,China,July 2020显示文摘Summary What is known about this topic?Few major outbreaks of coronavirus disease 2019(COVID-19)have occurred in China after major nonpharmaceutical interventions and vaccines have been deployed and implemented.However,sporadic outbreaks that had high possibility to be linked to cold chain products were reported in several cities of China..What is added by this report?In July 2020,a COVID-19 outbreak occurred in Dalian,China.The investigations of this outbreak strongly suggested that the infection source was from COVID-19 virus-contaminated packaging of frozen seafood during inbound unloading personnel contact.What are the implications for public health practice?Virus contaminated paper surfaces could maintain infectivity for at least 17–24 days at-25℃.Exposure to COVID-19 virus-contaminated surfaces is a potential route for introducing the virus to a susceptible population.Countries with no domestic transmission of COVID-19 should consider introducing prevention strategies for both inbound travellers and imported goods.Several measures to prevent the introduction of the virus via cold-chain goods can be implemented. | Huilai Ma1 Jianqun Zhang Ji Wang Ying Qin Cao Chen Yang Song Liang Wang Jun Meng Lingling Mao Fengqin Li Ning Li Jian Cai Yong Zhang Dayan Wang Yunting Xia Hong Wang Shaofeng Jiang Xiang Zhao Peihua Niu Wenjie Tan Tao Ma Yecheng Yao Naiying Mao Zhen Zhu Tianjiao Ji Qian Yang Baoying Huang Li Zhao Jianxing Yu Li Bai Shuangli Zhu Dongyan Wang Yan Zhang Yingwei Sun Mingchun Luan Yanhai Wang Haibo Sun Shihong Yang Zhijian Bo Xiang Ren Zhongjie Li George Fu Gao Wei Yao Wenqing Yao Zijian Feng Wenbo Xu | 2021 | China CDC weekly2021，3，21： | 16 |
| 15 | Effect of severe acute pancreatitis on pharmacokinetics of Da-Cheng-Qi Decoction components显示文摘AIM:To investigate the effect of severe acute pan- creatitis(SAP)on pharmacokinetics of Da-Cheng-Qi Decoction(DCQD)components in rats. METHODS:Rats were divided into SAP group and sham-operation group as a control group(n=6). Rhein,chrysophanol,rheochrysidin,magnolol,hesperidin and naringin in DCQD were quantified in rat serum by high performance liquid chromatography tandem mass spectrometry for studying their pharmacokinetics. RESULTS:Early absorption of each DCQD component was tended to degrade in SAP group after treatment with DCQD by gavage.The Cmax(chrysophanol,P= 0.0059;rheochrysidin,P=0.0288;magnolol,P= 0.0487;hesperidin,P=0.0277;naringin,P=0.0023) and AUC(rhein,P=0.0186;chrysophanol,P=0.0013; magnolol,P=0.001;hesperidin,P=0.0081;naringin, P=0.0272)of DCQD component were obviously lower in SAP group than in control group.The T1/2α of chrysophanol and rheochrysidin(P=0.0467 and 0.0005,respectively)and Tmax of chrysophanol and rheochrysidin(P=0.0101 and 0.0037,respectively) lasted longer in SAP group than in control group. CONCLUSION:SAP can significantly impact the ab-sorption of DCQD components in rats and their phar-macokinetic parameters. | Han-Lin Gong Wen-Fu Tang Qin Yu Jin Xiang Qing xia Guang-Yuan Chen Xi Huang Mao-Zhi Liang | 2009 | World Journal of Gastroenterology2009，15，47： | 16 |
| 16 | Deletion of an Endoplasmic Reticulum Stress Response Element in a ZmPP2C-A Gene Facilitates Drought Tolerance of Maize Seedlings显示文摘 | Yanli Xiang Xiaopeng Sun Shan Gao Feng Qin Mingqiu Dai | 2017 | Molecular Plant2017，10，3： | 15 |
| 17 | Effect of β2-adrenergic agonist clenbuterol on ischemia/reperfusion injury in isolated rat hearts and cardiomyocyte apoptosis induced by hydrogen peroxide显示文摘目的: 在 ischemia/reperfusion (I/R ) 上观察贝它 2-adrenergic 收缩筋 cienbuterol 的效果在孤立的老鼠心和过氧化氢(H2O2 ) 的损害 -in-duced cardiomyocyte apoptosis.Methods : 孤立的老鼠心受到 30 min 一台 Langendorff 仪器上的全球局部缺血和 60 min 灌注。心脏的功能被心搏率,左室的结束心脏舒张的压力(LVEDP ) ,左室的收缩压,左心室压(+dp/dt_( 最大)) 的最大的上升率和冠的自河(CF ) 评估。在冠的自河, malondialdehyde (MDA ) ,超级氧化物歧化酶(草皮) ,和在心脏的织物的 Ca^(2+)-ATPase 活动的 Lactate 脱氢酶(LDH ) 用商业工具包被测量。 apoptotic cardiomyocyte 被终端 deoxynucleotidyl 检测标记的 调停transferase 的 digoxigenin-dUTP 刻痕结束( TUNEL ) assay.Bax/Bcl-2 mRNA 层次和 caspase-3 的表达式被 RT-PCR 并且免疫弄污检测, respectively.Cultured 新生儿老鼠 cardiomyocytes 与 cienbuterol 被预先孵化,并且氧化压力损害被 H2O2.Cell 生存能力导致, cardiomyocyte apoptosis 被流动血细胞计数( FCM )评估 .Results :在在 I/R 损害以后的孤立的老鼠心, cienbuterol 显著地改进了心脏舒张的功能( LVEDP 和 CF )和Ca^(2+) -ATPase 活动。有 cienbuterol 的处理增加了草皮活动并且减少 MDA 水平和 LDH 与 I/R 组(P<0.05 ) 相比释放。而且, cienbuterol 减少了 apoptosis ,它在TUNEL积极的房间, Bax/Bcl-2 mRNA ,和 caspase-3 expres-sion.In 与减小被联系 导致H2O2 的 cardiomyocyte 损害, cienbuterol 增加了房间生存能力并且与 ICI118551 (选择贝它 2-adrenergic 对手)稀释了 cardiomyocyte apoptosis.Pre处理减少这些与 对待clenbuterol 的组( P<0.05 )相比完成 .Conclusion : Cienbuterol 改善了室的心脏舒张的功能由在 haning Ca^(2+) -ATPase 活动和减少的氧化应力和心脏的肌细胞,在一只试验性的老鼠的 apoptosis 当模特儿心肌层 I/R.It , H2O2 在 vitro.It 导致的减少的 cardiomyocyte apoptosis 对心肌层 I/R 损害在心脏的保护起一个关键作用。 | Ping LIU Ji-zhou XIANG Lei ZHAO Lei YANG Ben-rong HU Qin FU | 2008 | Acta Pharmacologica Sinica2008，29，6： | 14 |
| 18 | Variations of Laohugou Glacier No.12 in the western Qilian Mountains, China, from 1957 to 2015显示文摘Glaciers were solid reservoirs and important water resources in western China,but they were retreating significantly in context of global warming.Laohugou Glacier No.12 was the largest valley glacier in Qilian Mountains.In this study,realtime kinematic(RTK)data,topographic map and World View-2 satellite imagery were used to measure changes in terminus,extent and volume of Laohugou Glacier No.12.Results showed that Laohugou Glacier No.12 was shrinking significantly since 1957.From1960 to 2015,the terminus reduction of Laohugou Glacier No.12 was 402.96 m(3.99%)in total,and glacier length decreased to 9.7 km from 10.1 km.Reduction of glacier area and volume were the most obvious.From 1957 to 2015,glacier area and volume decreased by 1.54 km^2(7.03%)and 0.1816 km^3,respectively.Reduction trend of terminus and area was slowing in 1950-1980s,even stable for a period in the mid-1980s,and then accelerated.Ice core analysis result and nearly meteorological station data shown an increasing trend of temperature in 1957-2015,it was a main reason of continuous retreating of Laohugou Glacier No.12. | LIU Yu-shuo QIN Xiang CHEN Ji-zu LI Zhen-lin WANG Jing DU Wen-tao GUO Wan-qin | 2018 | Journal of Mountain Science2018，15，1： | 14 |
| 19 | Comprehensive treatments for hepatocellular carcinoma with tumor thrombus in major portal vein显示文摘AIM: To evaluate the efficacy of transcatheter arterial chemoembolisation(TACE) compared with surgical intervention and sorafenib for treatment of hepatocellular carcinoma(HCC) in patients with tumor thrombus extending to the main portal vein.METHODS: From 2009 to 2013, a total of 418 HCC patients with tumor thrombus extending to the main portal vein were enrolled in this study and divided into four groups. These groups underwent different treatments as follows: TACE(n = 307), surgical intervention(n = 54), sorafenib(n = 15) and palliativetreatment(n = 42). Overall survival rates were determined by Kaplan-Meier method, and differences between the groups were identified through log-rank analysis. Cox's proportional hazard model was used to identify the risk factors for survival.RESULTS: The mean survival periods for patients in the TACE, surgical intervention, sorafenib and palliative treatment groups were 10.39, 4.13, 5.54 and 2.82 mo, respectively. For the TACE group, the 3-, 6-, 12-and 24-mo survival rates were 94.1%, 85.9%, 51.5% and 0.0%, respectively. The corresponding rates were 60.3%, 22.2%, 0.0% and 0.0% for the surgical intervention group and 50.9%, 29.5%, 0.0% and 0.0% for the sorafenib group. Evidently, the results in the TACE group were significantly higher than those in the other groups(P < 0.0001). Furthermore, no significant difference among survival rates was observed between TACE with/without sorafenib(10.22 mo vs 10.52 mo, P = 0.615). No significant difference in survival rates was also found among the surgical intervention, sorafenib and palliative treatment groups(P > 0.05). These values significantly increased after TACE with/without sorafenib compared with other treatments(P < 0.05).CONCLUSION: For HCC patients with tumor thrombus extending to the main portal vein, TACE can yield a higher survival rate than surgical intervention or sorafenib treatment. | Hai-Hong Ye Jia-Zhou Ye Zhi-Bo Xie Yu-Chong Peng Jie Chen Liang Ma Tao Bai Jun-Ze Chen Zhan Lu Hong-Gui Qin Bang-De Xiang Le-Qun Li | 2016 | World Journal of Gastroenterology2016，22，13： | 14 |
| 20 | The Intervention Effect of Rosiglitozone in Ovarian Fibrosis of PCOS Rats显示文摘Objective To explore the Intervention effect of Rosiglitozone in ovarian fibrosis of PCOS rats.Methods 60 female SD rats were randomly divided into 3 groups:control group,model group and treatment group.The model and treatment groups were established by subcutaneous injection of DHEA,while the treatment group was given RGZ.The serum hormone values,pathohistology of ovarian structure of rats,ovarian ultrastructure and the expressions of TGF-β 1 and CTGF were detected.Results The PCOS model was established successfully.The expression intensity of TGF-β 1 and CTGF in Oocytes of the PCOS groups was 9.545±2.954 and 9.665±2.400,respectively and was significantly higher than that of the control group 6.636±2.264 and 7.036±2.133;after treatment with rosiglitazone,the expression was significantly decreased 6.980±2.421 and 6.642±2.721 as compared with that of the model group (P<0.05,P<0.001).The values in serum of the PCOS groups were 3.749±2.054 and 0.265±0.129,and 1.914±1.801 and 0.096±0.088 in the control group which had statistically significant difference (P<0.05,P<0.001).After treatment with rosiglitazone,the values were 2.3100±1.825 and 0.112±0.187 and were significantly different with those of the model group (P<0.05,P<0.001).Conclusion TGF-β 1 and CTGF play an important role in the development of ovary fibrosis in PCOS.However,RGZ may postpone the development of fibrosis by decreasing the levels of TGF-β 1 and CTGF. | MIAO Zhu Lin GUO Liang WANG Yong Xia CUI Rong YANG Ning HUANG Mi Qiong QIN Wei Bing CHEN Jin LI Hong Mei WANG Zi Neng WEI Xiang Cai | 2012 | Biomedical and Environmental Sciences2012，25，1： | 14 |
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