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| 1 | Molecular mechanism of SCARB2-mediatec attachment and uncoating of EV71显示文摘 | Minghao Dang Xiangxi Wang Quan Wang Yaxin Wang Jianping Lin Yuna Sun Xuemei Li Liguo Zhang Zhiyong Lou Junzhi Wang Zihe Rao | 2014 | Protein & Cell2014，5，9： | 13 |
| 2 | The binding of a monoclonal antibody to the apical region of SCARB2 blocks EV71 infection显示文摘(EV71 ) Entero 病毒 71 引起手,脚,和嘴疾病(HFMD ) 并且偶尔导致严重神经病学的复杂并发症甚至死亡。(SCARB2 ) Scavenger 受体班 B 成员 2 是为 EV71 的功能的受体,调停病毒的附件,成为主观,和 uncoating。然而, SCARB2 上的 EV71 的准确有约束力的地点是未知的。在这研究,我们产生了绑在人然而并非老鼠 SCARB2 的 monoclonal 抗体(mAb ) 。它被称为 JL2,并且它能有效地禁止目标房间的 EV71 感染。用人和老鼠 SCARB2 的一套怪物,我们鉴别包含人的 SCARB2 的残余 77-113 的区域显著地作出贡献到 JL2 绑定。SCARB2-JL2 建筑群的结构表明 JL2 绑在包含 -helices 的 SCARB2 的顶端的区域 2, 5,和 14。我们的结果在 SCARB2 和 EV71 入口的分子的机制上为 EV71 提供新卓见进潜在的有约束力的地点。 | Xuyuan Zhang Pan Yang Nan Wang Jialong Zhang Jingyun Li Hao Guo Xiangyun Yin Zihe Rao Xiangxi wang Liguo Zhang | 2017 | Protein & Cell2017，8，8： | 10 |
| 3 | Neomycin biosynthesis is regulated positively by AfsA-g and NeoR in Streptomyces fradiae CGMCC 4.7387显示文摘Neomycins are a group of aminoglycoside antibiotics with both clinical and agricultural applications.To elucidate the regulatory mechanism of neomycin biosynthesis,we completed draft genome sequencing of a neomycin producer Streptomyces fradiae CGMCC 4.7387 from marine sediments,and the neomycin biosynthesis gene cluster was identified.Inactivation of the afsA-g gene encoding a γ-butyrolactone(GBL) synthase in S.fradiae CGMCC 4.7387 resulted in a significant decrease of neomycin production.Quantitative RT-PCR analysis revealed that the transcriptional level of neoR and the aphA-neoGH operon were reduced in the afsA-g::aac(3)Ⅳ mutant.Interestingly,a conserved binding site of AdpA,a key activator in the GBL regulatory cascade,was discovered upstream of neoR,a putative regulatory gene encoding a protein with an ATPase domain and a tetratricopeptide repeat domain.When neoR was inactivated,the neomycin production was reduced about 40%in comparison with the WT strain.Quantitative RT-PCR analysis revealed that the transcriptional levels of genes in the aphA-neoGH operon were reduced clearly in the neoR::aac(3)Ⅳ mutant.Finally,the titers of neomycin were improved considerably by overexpression of qfsA-gand neoR in S.fradiae CGMCC 4.7387. | Xiangxi Meng Wenzhao Wang Zhoujie Xie Pengwei Li Yue Li Zhengyan Guo Yingjian Lu Jie Yang Kaile Guan Zhaoxin Lu Huarong Tan Yihua Chen | 2017 | Science China(Life Sciences)2017，60，9： | 7 |
| 4 | TIM-1 acts a dual-attachment receptor for Ebolavirus by interacting directly with viral GP and the PS on the viral envelope显示文摘Ebolavirus 能与 50%90% 的死亡率在人引起出血性的发烧。当前,没有同意的疫苗和抗病毒的治疗是可得到的。人的 TIM1 为 EBOV 被看作一个附件因素,提高通过和位于病毒的信封的 PS 的相互作用的病毒的感染。然而,推理由 filovirus 位于 hTIM-1,然而并非另外的 PS 有约束力的受体的更好的用法下面,未知的遗体。我们第一在 vitro 表明了在 hTIM-1 和 EBOV GP 之间的一个直接相互作用并且决定了 hTIM-1 和 hTIM-4 的 Ig V 领域的水晶结构。在到 EBOV GP 的 hTIM-1 的有约束力的区域被怪物和变化试金印射,它基于结构的分析被设计。象点异种一样用 hTIM-1 和它的相当或相同的事物执行的 Pseudovirion 感染试金在 EBOV 细胞的入口验证了 GP 有约束力的地点和 EBOV GP-hTIM-1 相互作用的重要性的地点。 | Shuai Yuan Lei Cao Hui Ling Minghao Dang Yao Sun Xuyuan Zhang Yutao Chen Liguo Zhang Dan Su Xiangxi Wang Zihe Rao | 2015 | Protein & Cell2015，6，11： | 6 |
| 5 | Numerical simulation of the dimensional transformation of atomization in a supersonic aerodynamic atomization dust-removing nozzle based on transonic speed compressible flow显示文摘To simulate the transonic atomization jet process in Laval nozzles,to test the law of droplet atomization and distribution,to find a method of supersonic atomization for dust-removing nozzles,and to improve nozzle efficiency,the finite element method has been used in this study based on the COMSOL computational fluid dynamics module.The study results showed that the process cannot be realized alone under the two-dimensional axisymmetric,three-dimensional and three-dimensional symmetric models,but it can be calculated with the transformation dimension method,which uses the parameter equations generated from the two-dimensional axisymmetric flow field data of the three-dimensional model.The visualization of this complex process,which is difficult to measure and analyze experimentally,was realized in this study.The physical process,macro phenomena and particle distribution of supersonic atomization are analyzed in combination with this simulation.The rationality of the simulation was verified by experiments.A new method for the study of the atomization process and the exploration of its mechanism in a compressible transonic speed flow field based on the Laval nozzle has been provided,and a numerical platform for the study of supersonic atomization dust removal has been established. | Tian Zhang Deji Jing Shaocheng Ge Jiren Wang Xiangxi Meng ShuaiShuai Ren | 2020 | International Journal of Coal Science & Technology2020，7，3： | 6 |
| 6 | Architecture of the herpesvirus genomepackaging complex and implications for DNA translocation显示文摘Genome packaging is a fundamental process in a viral life cycle and a prime target of antiviral drugs.Herpesviruses use an ATP-driven packaging motor/terminase complex to translocate and cleave concatemeric dsDNA into procapsids but its molecular architecture and mechanism are unknown.We report atomic structures of a herpesvirus hexameric terminase complex in both the apo and ADP·BeF3-bound states.Each subunit of the hexameric ring comprises three components-the ATPase/terminase pUL15 and two regulator/fixer proteins,pUL28 and pUL33-unlike bacteriophage terminases.Distal to the nuclease domains,six ATPase domains form a central channel with conserved basicpatches conducive to DNA binding and trans-acting arginine fingers are essential to ATP hydrolysis and sequential DNA translocation.Rearrangement of the nuclease domains mediated by regulatory domains converts DNA translocation mode to cleavage mode.Our structures favor a sequential revolution model for DNA translocation and suggest mechanisms for concerted domain rearrangements leading to DNA cleavage. | Yunxiang Yang Pan Yang Nan Wang Zhonghao Chen Dan Su ZHong Zhou Zihe Rao Xiangxi Wang | 2020 | Protein & Cell2020，11，5： | 5 |
| 7 | Rational development of a human antibody cocktail that deploys multiple functions to confer Pan-SARS-CoVs protection显示文摘Structural principles underlying the composition and synergistic mechanisms of protective monoclonal antibody cocktails are poorly defined.Here,we exploited antibody cooperativity to develop a therapeutic antibody cocktail against SARS-CoV-2.On the basis of our previously identified humanized cross-neutralizing antibody H014,we systematically an a lyzed a fully human n aive antibody library and rationally identified a potent neutralizing antibody partner,P17,which confers effective protection in animal model.Cryo-EM studies dissected the nature of the P17 epitope,which is SARS-CoV-2 specific and distinctly different from that of HOI4.High-resolution structure of the SARS-CoV-2 spike in complex with HOM and P17,together with functional investigations revealed that in a two-antibody cocktail,synergistic neutralization was achieved by S1 shielding and conformational locking,thereby blocking receptor attachment and viral membrane fusion,conferring high potency as well as robustness against viral mutation escape.Furthermore,cluster analysis identified a hypothetical 3rd antibody partner for further reinforcing the cocktail as pan-SARS-CoVs therapeutics. | Hangping Yao Yao Sun Yong-Qiang Deng Nan Wang Yongcong Tan Na-Na Zhang Xiao-Feng Li Chao Kong Yan-Peng Xuc Qi Chen Tian-Shu Cao Hui Zhao Xintian Yan Lei Cao Zhe Lv Dandan Zhu Rui Feng Nanping Wu Wenhai Zhang Yuhao Hu Keda Chen Rong-Rong Zhang Qingyu Lv Shihui Sun Yunhua Zhou Run Yan Guan Yang Xinglu Chanjuan Liu Xiangyun Lu Linfang Cheng Hongying Qiu Xing-Yao Huang Tianhao Weng Danrong Shi Weidong Jiang Junbin Shao Lei Wang Jie Zhang Tao Jiang Guojun Lang Cheng-Feng Qinc Lanjuan Li Xiangxi Wang | 2021 | Cell Research2021，31，1： | 5 |
| 8 | Structure-based development of human antibody cocktails against SARS-CoV-2显示文摘Dear Editor,The on going COVID-19 pandemic caused by severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)has resulted in unprecedented public health and socioeconomic crises,requiring urgent developments of effective COVID-19 therapeutics and vaccines.Humoral immunity is essential for protection against coronavirus infections and passive immunization has been demonstrated to be effective in curing SARS-CoV-2-challenged non human primates.1,2 A deep understanding of the molecular basis of neutralizing antibody(NAb)responses to SARS-CoV-2 could facilitate vaccine design and drug discovery. | Nan Wang Yao Sun Rui Feng Yuxi Wang Yan Guo Li Zhang Yong-Qiang Deng Lei Wang Zhen Cui Lei Cao Yan-Jun Zhang Weimin Li Feng-Cai Zhu Cheng-Feng Qin Xiangxi Wang | 2021 | Cell Research2021，31，1： | 5 |
| 9 | Ultrafine Sn_(4)P_(3)nanocrystals from chloride reduction on mechanically activated Na surface for sodium/lithium ion batteries显示文摘Nanostructured metal phosphides are very attractive materials in energy storage and conversion,but their applications are severely limited by complicated preparation steps,harsh conditions and large excess of highly toxic phosphorus source.Here we develop a highly efficient one-step method to synthesize Sn_(4)P_(3)nanostructure based on simultaneous reduction of SnCl_(4)and PCl_(3)on mechanically activated Na surface and in situ phosphorization.The low-toxic PCl3 displays a very high phosphorizing efficiency(100%).Furthermore,this simple method is powerful to control phosphide size.Ultrafine Sn_(4)P_(3)nanocrystals(<5 nm)supported on carbon sheets(Sn_(4)P_(3)/C)are obtained,which is due to the unique bottom-up surface-limited reaction.As the anode material for sodium/lithium ion batteries(SIBs/LIBs),the Sn_(4)P_(3)/C shows profound sodiation/lithiation extents,good phase-conversion reversibility,excellent rate performance and long cycling stability,retaining high capacities of 420 mAh/g for SIBs and 760 mAh/g for LIBs even after 400 cycles at 1.0 A/g.Combining simple and efficient preparation,low-toxic and high-efficiency phosphorus source and good control of nanosize,this method is very promising for low-cost and scalable preparation of high-performance Sn_(4)P_(3)anode. | Zhiliang Liu Xiangxi Wang Zhuoyan Wu Sungjin Yang Shaolei Yang Shunpeng Chen Xinteng Wu Xinghua Chang Piaoping Yang Jie Zheng Xingguo Li | 2020 | Nano Research2020，13，11： | 4 |
| 10 | A proof of concept for neutralizing antibody-guided vaccine design against SARS-CoV-2显示文摘Mutations and transient conformational movements of the receptor binding domain(RBD)that make neutralizing epitopes momentarily unavailable present immune escape routes for severe acute respiratory syndrome coronavirus 2(SARS-CoV-2).To mitigate viral escape,we developed a cocktail of neutralizing antibodies(NAbs)targeting epitopes located on different domains of spike(S)protein.Screening of a library of monoclonal antibodies generated from peripheral blood mononuclear cells of COVID-19 convalescent patients yielded potent NAbs,targeting the N-terminal domain(NTD)and RBD domain of S,effective at n M concentrations.Remarkably,a combination of RBD-targeting NAbs and NTD-binding NAbs,FC05,enhanced the neutralization potency in cell-based assays and an animal model.Results of competitive surface plasmon resonance assays and cryo-electron microscopy(cryo-EM)structures of antigen-binding fragments bound to S unveil determinants of immunogenicity.Combinations of immunogens,identified in the NTD and RBD of S,when immunized in rabbits and macaques,elicited potent protective immune responses against SARS-CoV-2.More importantly,two immunizations of this combination of NTD and RBD immunogens provided complete protection in macaques against a SARS-CoV-2 challenge,without observable antibody-dependent enhancement of infection.These results provide a proof of concept for neutralization-based immunogen design targeting SARS-CoV-2 NTD and RBD. | Li Zhang Lei Cao Xing-Su Gao Bin-Yang Zheng Yong-Qiang Deng Jing-Xin Li Rui Feng Qian Bian Xi-Ling Guo Nan Wang Hong-Ying Qiu Lei Wang Zhen Cui Qing Ye Geng Chen Kui-Kui Lu Yin Chen Yu-Tao Chen Hong-Xing Pan Jiaping Yu Wenrong Yao Bao-Li Zhu Jianping Chen Yong Liu Cheng-Feng Qin Xiangxi Wang Feng-Cai Zhu | 2021 | National Science Review2021，8，8： | 4 |
| 11 | An open conformation determined by a structural switch for 2A protease from coxsackievirus A16显示文摘Coxsackievirus A16 belongs to the family Picornaviridae,and is a major agent of hand-foot-and-mouth disease that infects mostly children,and to date no vaccines or antivi-ral therapies are available.2A protease of enterovirus is a nonstructural protein and possesses both self-cleavage activity and the ability to cleave the eukaryotic translation initiation factor 4G.Here we present the crystal structure of coxsackievirus A162A protease,which interestingly forms hexamers in crystal as well as in solution.This structure shows an open conformation,with its active site accessible,ready for substrate binding and cleav-age activity.In conjunction with a previously reported“closed”state structure of human rhinovirus 2,we were able to develop a detailed hypothesis for the conforma-tional conversion triggered by two“switcher”residues Glu88 and Tyr89 located within the bll2-cII loop.Substrate recognition assays revealed that amino acid residues P1′,P2 and P4 are essential for substrate specificity,which was verifi ed by our substrate binding model.In addition,we compared the in vitro cleavage effi ciency of 2A pro-teases from coxsackievirus A16 and enterovirus 71 upon the same substrates by fl uorescence resonance energy transfer(FRET),and observed higher protease activity of enterovirus 71 compared to that of coxsackievirus A16.In conclusion,our study shows an open conformation of coxsackievirus A162A protease and the underlying mechanisms for conformational conversion and substrate specifi city.These new insights should facilitate the future rational design of effi cient 2A protease inhibitors. | Yao Sun Xiangxi Wang Shuai Yuan Minghao Dang Xuemei Li Xuejun CZhang Zihe Rao | 2013 | Protein & Cell2013，4，10： | 3 |
| 12 | Structural and molecular basis for foot-andmouth disease virus neutralization by two potent protective antibodies显示文摘Dear Editor,Foot-and-mouth disease(FMD)is an economically devastating and highly contagious viral disease of cloven-hoofed animals with a global distribution.The causative agent,FMD virus(FMDV)is a small non-enveloped RNA virus,belonging to the Aphthoviruses genus within Picornaviridae family(Tuthill et al.,2010).Control of FMD has been largely reliant on vaccinations with inactivated virus vaccines.However,significant antigenic diversity within FMDV serotypes and inability of the vaccines to induce immune protection for a long duration of time impinge on the efficacy of available vaccines.The roles of neutralizing antibodies(NAbs)as the principal protective components of the immune responses to FMDV vaccination or infection have been well established(Pay and Hingley,1987;Juleff et al.,2009).Passive immunization of NAbs has also been demonstrated to be effective in curing FMD and many viral diseases(Harmsen et al.,2007;Qiu et al.,2018).A deep understanding of the molecular basis for viral neutralization by antibodies and the identification of key viral epitopes would aid in the development of potent rationally designed broad-spectrum vaccine. | Hu Dong Pan Liu Manyuan Bai Kang Wang Rui Feng Dandan Zhu Yao Sun Suyu Mu Haozhou Li Michiel Harmsen Shiqi Sun Xiangxi Wang Huichen Guo | 2022 | Protein & Cell2022，13，6： | 3 |
| 13 | Double lock of a potent human therapeutic monoclonal antibody against SARS-CoV-2显示文摘Receptor recognition and subsequent membrane fusion are essential for the establishment of successful infection by SARS-CoV-2.Halting these steps can cure COVID-19.Here we have identified and characterized a potent human monoclonal antibody,HB27,that blocks SARS-CoV-2 attachment to its cellular receptor at sub-nM concentrations.Remarkably,HB27 can also prevent SARS-CoV-2 membrane fusion.Consequently,a single dose of HB27 conferred effective protection against SARS-CoV-2 in two established mouse models.Rhesus macaques showed no obvious adverse events when administrated with10 times the effective dose of HB27.Cryo-EM studies on complex of SARS-CoV-2 trimeric S with HB27 Fab reveal that three Fab fragments work synergistically to occlude SARS-CoV-2 from binding to the ACE2 receptor.Binding of the antibody also restrains any further conformational changes of the receptor binding domain,possibly interfering with progression from the prefusion to the postfusion stage.These results suggest that HB27 is a promising candidate for immuno-therapies against COVID-19. | Ling Zhu Yong-Qiang Deng Rong-Rong Zhang Zhen Cui Chun-Yun Sun Chang-Fa Fan Xiaorui Xing Weijin Huang Qi Chen Na-Na Zhang Qing Ye Tian-Shu Cao Nan Wang Lei Wang Lei Cao Huiyu Wang Desheng Kong Juan Ma Chunxia Luo Yanjing Zhang Jianhui Nie Yao Sun Zhe Lv Neil Shaw Qianqian Li Xiao-Feng Li Junjie Hu Liangzhi Xie Zihe Rao Youchun Wang Xiangxi Wang Cheng-Feng Qin | 2021 | National Science Review2021，8，3： | 3 |
| 14 | SARS-CoV-2 spike protein interacts with and activates TLR41显示文摘Dear Editor,Accumulating clinical data suggest the main causes of death by COVID-19 include respiratory failure and the onset of sepsis.1 Importantly,sepsis has been observed in nearly all deceased patients.2,3,4,5 It remains elusive how SARS-CoV-2 infection results in viral sepsis in humans.Toll-like receptor 4(TLR4)mediates anti-gram-negative bacterial immune responses by recognizing lipopolysaccharide(LPS)from bacteria.6 We recently found that SARS-CoV-2 infection provoked an anti-bacterial like response at the very early stage of infection via TLR4.However,the identity of the original trigger initiating these abnormal immune responses during SARS-CoV-2 infection is unknown. | Yingchi Zhao Ming Kuang Junhong Li Ling Zhu Zijing Jia Xuefei Guo Yaling Hu Jun Kong Hang Yin Xiangxi Wang Fuping You | 2021 | Cell Research2021，31，7： | 3 |
| 15 | Structures of the portal vertex reveal essential protein-protein interactions for Herpesvirus assembly and maturation显示文摘Dear Editor,Herpesviridae is a large family of double-stranded DNA(dsDNA)viruses that cause a variety of human diseases ranging from cold sores and chicken pox to congenital defects,blindness and cancer(Chayavichitsilp et al.,2009;Wang et al.,2018).In the past 70 years,substantial advances in our knowledge of the molecular biology of herpesviruses have led to insights into disease pathogenesis and management.However,the mechanism for capsid assembly that requires the ordered packing of about 4,000 protein subunits into the hexons,pentons and triplexes remains elusive.It is still a puzzle how initially identical subunits adopt both hexameric and pentameric conformations in the capsid and select the correct locations needed to form closed shells of the proper size.Biochemical and genetic studies have shown that the portal is involved in initiation of capsid assembly(Newcomb et al.,2005)and functions akin to a DNA-sensor coupling genome-packaging achieved by a genome-packaging machinery-“terminase complex”(Chen et al.,2020;Yunxiang Yang,2020)with icosahedral capsid maturation(Lokareddy et al.,2017).Structural investigations of the herpesvirus portal have proven challenging due to the small size of this dodecamer,which accounts for less than 1%of the total mass of the capsid protein layer and the technical difficulties involved in resolving non-icosahedral components of such large icosahedral viruses(diameter is∼1,250Å).Efforts of many investigators over two decades have made to reconstruct the cryo-electron microscopy(cryo-EM)structure of herpesvirus portal vertex and more recently near-atomic structures of two herpesvirus(herpes simplex virus type 1(HSV-1)and Kaposi’s sarcoma-associated herpesvirus(KSHV))portal vertices were reported(McElwee et al.,2018;Gong et al.,2019;Liu et al.,2019). | Nan Wang Wenyuan Chen Ling Zhu Dongjie Zhu Rui Feng Jialing Wang Bin Zhu Xinzheng Zhang Xiaoqing Chen Xianjie Liu Runbin Yan Dongyao Ni Grace Guoying Zhou Hongrong Liu Zihe Rao Xiangxi Wang | 2020 | Protein & Cell2020，11，5： | 2 |
| 16 | Sequential immunizations confer cross-protection against variants of SARS-CoV-2,including Omicron in Rhesus macaques显示文摘Variants of concern(VOCs)like Delta and Omicron,harbor a high number of mutations,which aid these viruses in escaping a majority of known SARS-CoV-2 neutralizing antibodies(NAbs).In this study,Rhesus macaques immunized with 2-dose inactivated vaccines(Coronavac)were boosted with an additional dose of homologous vaccine or an RBD-subunit vaccine,or a bivalent inactivated vaccine(Beta and Delta)to determine the effectiveness of sequential immunization.The booster vaccination significantly enhanced the duration and levels of neutralizing antibody titers against wild-type. | Wei Deng Qi Lv Fengdi Li Jiangning Liu Zhiqi Song Feifei Qi Qiang Wei Pin Yu Mingya Liu Shasha Zhou Yaqing Zhang Hong Gao Nan Wang Zijing Jia Kai Gao Jiayi Liu Chong Xiao Haiquan Shang Xiangxi Wang Linlin Bao Chuan Qin | 2022 | Signal Transduction and Targeted Therapy2022，7，5： | 2 |
| 17 | Rock borehole shear tests in dam foundation of Xiangjiaba hydropower station显示文摘Xiangjiaba hydropower station is one of the cascade power stations on the Jinsha River, China. Due to the complicated geological conditions of its dam foundation, evaluating the rock mass quality and determining the mechanical parameters of rock masses are very important issues. To address these issues, several groups of rock borehole shear tests (RBSTs) were conducted on the black mudstone in the dam foundation of Xiangjiaba hydropower station in the second construction phase. Forty three groups of shear strengths of black mudstone samples were obtained from RBSTs, and the shear strength parameters (c and f ) were calculated using the least squares method. In addition, the limitations and merits of RBST employed in the Xiangjiaba hydropower station were discussed. Test results indicate that the shear strength parameters obtained from RBST have a good correlation with the results from sound wave test in borehole. It is believed that RBST has a good adaptability and applicability in geotechnical engineering. | Yufei Zhao Xiaogang Wang Xiaohui Zhang Zhixin Jia Xiangxi Zeng Hongtao Zhang | 2012 | Journal of Rock Mechanics and Geotechnical Engineering2012，4，4： | 2 |
| 18 | Structure-based development of three- and four-antibody cocktails against SARS-CoV-2 via multiple mechanisms显示文摘The ongoing coronavirus disease 2019(COVID-19)pandemic caused by severe acute respiratory syndrome coronavirus-2(SARS-CoV-2)has resulted in an unprecedented public health crisis,galvanizing a global effort for rapidly developing new therapeutic strategies effective against COVID-19.Human monoclonal antibodies(mAbs)are promising therapeutic molecules that can be used for the prevention or treatment of viral infectious diseases,including COVID-19.For instance,ZMapp^(TM),a cocktail consisting of three different mAbs targeting the Ebola glycoprotein is one of the most successful antibody-based therapeutic for treating infections caused by Ebola virus.1 Notably,this cocktail combines the best-performing neutralizing antibodies(NAbs)screened and developed using two separate approaches,one from humanized antibodies of origin and the other from human survivors. | Yao Sun Lei Wang Rui Feng Nan Wang Yuxi Wang Dandan Zhu Xiaorui Xing Peng Yang Yanjun Zhang Weimin Li Xiangxi Wang | 2021 | Cell Research2021，31，5： | 2 |
| 19 | Blade Segment with a 3D Lattice of Diamond Grits Fabricated via an Additive Manufacturing Process显示文摘Diamond tools with orderly arrangements of diamond grits have drawn considerable attention in the machining field owing to their outstanding advantages of high sharpness and long service life.This diamond super tool,as well as the manufacturing equipment,has been unavailable to Chinese enterprises for a long time due to patents.In this paper,a diamond blade segment with a 3D lattice of diamond grits was additively manufactured using a new type of cold pressing equipment(AME100).The equipment,designed with a rotary working platform and 16 molding stations,can be used to additively manufacture segments with diamond grits arranged in an orderly fashion,layer by layer;under this additive manufacturing process,at least 216000 pcs of diamond green segments with five orderly arranged grit layers can be produced per month.The microstructure of the segment was observed via SEM and the diamond blade fabricated using these segments was compared to other commercial cutting tools.The experimental results showed that the 3D lattice of diamond grits was formed in the green segment.The filling rate of diamond grits in the lattice could be guaranteed to be above 95%;this is much higher than the 90%filling rate of the automatic array system(ARIX).When used to cut stone,the cutting amount of the blade with segments made by AME100 is two times that of ordinary tools,with the same diamond concentration.When used to dry cut reinforced concrete,its cutting speed is 10%faster than that of ARIX.Under wet cutting conditions,its service life is twice that of ARIX.By applying the machine vision online inspection system and a special needle jig with a negative pressure system,this study developed a piece of additive manufacturing equipment for efficiently fabricating blade segments with a 3D lattice of diamond grits. | Bin Chen Peng Chen Yongjun Huang Xiangxi Xu Yibo Liu Shuangxi Wang | 2020 | Chinese Journal of Mechanical Engineering2020，33，5： | 1 |
| 20 | Publisher Correction: SARS-CoV-2 spike protein interacts with and activates TLR4显示文摘We apologize for an error in this paper online published on Mar 19,2021.There is a mistake on the title where“TRL41”should be“TLR4”.This correction does not affect the description of the results or the conclusion of this work.We apologize for not detecting them before publication and any inconvenience caused.The correct title should be as follows. | Yingchi Zhao Ming Kuang Junhong Li Ling Zhu Zijing Jia Xuefei Guo Yaling Hu Jun Kong Hang Yin Xiangxi Wang Fuping You | 2021 | Cell Research2021，31，7： | 1 |
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