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| 1 | Embryonic endothelial evolution towards first hematopoietic stem cells revealed by single-cell transcriptomic and functional analyses显示文摘Hematopoietic stem cells(HSCs)in adults are believed to be born from hemogenic endothelial cells(HECs)in mid-gestational embryos.Due to the rare and transient nature,the HSC-competent HECs have never been stringently identified and accurately captured,let alone their genuine vascular precursors.Here,we first used high-precision single-cell transcriptomics to unbiasedly examine the relevant EC populations at continuous developmental stages with intervals of 0.5 days from embryonic day(E)9.5 to E11.0.As a consequence,we transcriptomically identified two molecularly different arterial EC populations and putative HSCprimed HECs,whose number peaked at El 0.0 and sharply decreased thereafter,in the dorsal aorta of the aorta-gonadmesonephros(AGM)region.Combining computational prediction and in vivo functional validation,we precisely captured HSCcompetent HECs by the newly constructed Neurl3-EGFP reporter mouse model,and realized the enrichment further by a combination of surface markers(Procr+Kit+CD44+,PK44).Surprisingly,the endothelial-hematopoietic dual potential was rarely but reliably witnessed in the cultures of single HECs.Noteworthy,primitive vascular ECs from E8.0 experienced two-step fate choices to become HSC-primed HECs,namely an Initial arterial fate choice followed by a hemogenic fate conversion.This finding resolves several previously observed contradictions.Taken together,comprehensive understanding of endothelial evolutions and molecular programs underlying HSC-primed HEC specification in vivo will facilitate future investigations directing HSC production in vitro. | Siyuan Hou Zongcheng Li Xiaona Zheng Yun Gao Ji Dong Yanli Ni Xiaobo Wang Yunqiao Li Xiaochen Ding Zhilin Chang Shuaili Li Yuqiong Hu Xiaoying Fan Yu Hou Lu Wen Bing Liu Fuchou Tang Yu Lan | 2020 | Cell Research2020，30，5： | 8 |
| 2 | A newly developed ocean significant wave height retrieval method from Envisat ASAR wave mode imagery显示文摘The main objective of this paper is to propose a newly developed ocean Significant Wave Height (SWH) retrieval method from Envisat Advanced Synthetic Aperture Radar (ASAR) imagery. A series of wave mode imagery from January, April and May of 2011 are collocated with ERA-Interim reanalysis SWH data. Based on the matched datasets, a simplified empirical relationship between 22 types of SAR imagery parameters and SWH products is developed with the Genetic Algorithms Partial Least-Squares (GA-PLS) model. Two major features of the backscattering coefficient σ0 and the frequency parameter S10 are chosen as the optimal training feature subset of SWH retrieval by using cross validation. In addition, we also present a comparison of the retrieval results of the simplified empirical relationship with the collocated ERA-Interim data. The results show that the assessment index of the correlation coefficient, the bias, the root-mean-square error of cross validation (RMSECV) and the scattering index (SI) are 0.78, 0.07 m, 0.76 m and 0.5, respectively. In addition, the comparison of the retrieved SWH data between our simplifying model and the Jason-2 radar altimeter data is proposed in our study. Moreover, we also make a comparison of the retrieval of SWH data between our developed model and the well- known CWAVE_ENV model. The results show that satisfying retrieval results are acquired in the low-moderate sea state, but major bias appears in the high sea state, especially for SWH>5 m. | Chenqing Fan Xiaochen Wang Xudong Zhang Dong Gao | 2019 | Acta Oceanologica Sinica2019，38，9： | 2 |
| 3 | Heterogeneity in endothelial cells and widespread venous arterialization during early vascular development in mammals显示文摘Arteriogenesis rather than unspecialized capillary expansion is critical for restoring effective circulation to compromised tissues in patients.Deciphering the origin and specification of arterial endothelial cells during embryonic development will shed light on the understanding of adult arteriogenesis.However,during early embryonic angiogenesis,the process of endothelial diversification and molecular events underlying arteriovenous fate settling remain largely unresolved in mammals.Here,we constructed the single-cell transcriptomic Iandscape of vascular endothelial cells(VECs)during the time window for the occurrenee of key vasculogenic and angiogenic events in both mouse and human embryos.We uncovered two distinct arterial VEC types,the major artery VECs and arterial plexus VECs,and un expectedly diverge nt arteriove nous characteristics among VECs that are located in morphologically undistinguishable vascular plexus intra-embryonically.Using computational prediction and further lineage tracing of venous-featured VECs with a newly developed Nr2f2^(CrexER) | Siyuan Hou Zongcheng Li Ji Dong Yun Gao Zhilin Chang Xiaochen Ding Shuaili Li Yunqiao Li Yang Zeng Qian Xin Baihan Wang Yanli Ni Xiaowei Ning Yuqiong Hu Xiaoying Fan Yu Hou Xianlong Li Lu Wen Bin Zhou Bing Liu Fuchou Tang Yu Lan | 2022 | Cell Research2022，32，4： | 2 |
| 4 | Hydrogenated vanadium oxides as an advanced anode material in lithium ion batteries显示文摘 | Yufei Zhang Huanwen Wang Jun Yang Haosen Fan Yu Zhang Zhengfei Dai Yun Zheng Wei Huang Xiaochen Dong Qingyu Yan | 2017 | Nano Research2017，10，12： | 2 |
| 5 | Indirect Measurement Methods forQuality and Process Control in Nanomanufacturing显示文摘Rapid advancement over the past decades in nanomanufacturing has led to the realization of a broad range of nanostructures such as nanoparticles,nanotubes,and nanowires.The unique mechanical,chemical,and electrical properties of these nanostructures have made them increasingly desired as key components in industrial and commercial applications.As the geometric dimension of nano-manufactured products is on the sub-micron to nanometer scale,different mechanisms and effects are involved in the nanomanufacturing process as compared to those for macro-scale manufacturing.Although direct measurement methods using atomic force microscopy and electron beam microscopy can determine the dimensions of the nano structure with high accuracy,these methods are not suited for online process control and quality assurance.In comparison,indirect measurement methods analyze in-process parameters as the basis for inferring the dimensional variations in the nano products,thereby enabling online feedback for process control and quality assurance.This paper provides a comprehensive review of relevant indirect measurement methods,starting with their respective working principles,and subsequently discussing their characteristics and applications in terms of two different approaches:data-based and physicsbased methods.Relevant mathematical and physics models for each of the methods are summarized,together with the associated effect of key process parameters on the quality of the final product.Based on the comprehensive literature conducted,it was found that:(1)indirect measurement,especially the data-based method,plays a critical role when it comes to online process control and quality assurance in nanomanufacturing,because of the short processing time compared to the direct method,and(2)physics-based method is providing a way to optimize the process set up for desired geometrical dimensions. | Zhaoyan Fan Xiaochen Hu Robert X.Gao | 2022 | Nanomanufacturing and Metrology2022，5，3： | 1 |
| 6 | Development of new endovascular stent-graft system for type B thoracic aortic dissection with finite element analysis and experimental verification显示文摘Endovascular repair of the thoracic aorta with self-expanding stent-grafts has been emerging as a less invasive alternative treatment compared with conventional open surgeries.Despite the promising efficacy and safety of endovascular stent grafting,the stent-graft failure remains a major concern in terms of stent migration,device fatigue,and the risk of endoleaks.Challenges associated with the stent-grafts involve optimized geometrical structure,lifetime fatigue resistance,and adequate radial support.In this work,a novel endovascular stent-graft system is developed specially for the treatment of Stanford type B thoracic aortic dissections(TAD).Numerical study with finite element analysis(FEA)was utilized to evaluate the mechanical behaviors of the individual stent component.Results of the simulation were validated by experimental tests.Based on the systematic analysis of the parametric variations,a final stent-graft system was developed by the selection and arrangement of the individual stent components,targeting an optimal performance for treatment of TAD.The optimized solution of the stent-graft system was tested in clinical trials,showing advantageous therapeutic efficacy. | Xiaochen Zhou Fan Yang Xiaoyan Gong Ming Zhao Yufeng Zheng Zhili Sun | 2019 | Journal of Materials Science & Technology2019，35，11： | 1 |
| 7 | A small molecular electron acceptor based on asymmetric hexacyclic core of thieno[1,2-b]indaceno[5,6-b']thienothiophene for efficient fullerene-free polymer solar cells显示文摘A novel A-D-A(acceptor-donor-acceptor)type non-fullerene small molecule,A201,consisting of an asymmetric thieno[1,2-b]indaceno[5,6-b^0]thienothiophene(TITT)unit as middle D part and 2-(3-oxo-2,3-dihydroinden-1-ylidene)malononitrile(IC)groups as end-capped A parts was designed and synthesized.The asymmetric TITT building block showed a higher dipole moment of 0.85 Debye(1 Debye=3.33564?10^(à30)cm)compared with the symmetric analogues of indacenodithiophene(IDT)and indacenodithieno[3,2-b]thiophene(IDTT)of 0.098 and 0.13 Debye,respectively.The solution-processed bulk heterojunction solar cells using a benzotriazole(BTA)-based polymer of J71 as donor and A201 as acceptor,showed a power conversion efficiency(PCE)of 9.36%with an open-circuit voltage(V_(oc))of0.88 V,a short-circuit current(J_(sc))of 13.15 m A cm^(à2),and a fill factor(FF)of 0.67,under the illumination of AM 1.5G at 100 m W cm^(à2).The high PCE of this material combination could be attributed to its broad absorption spectrum and the high hole mobility(l_h)and electron mobility(l_e)of 9.56?10^(à4)and 5.17?10^(à4)cm^2V^(à1)s^(à1),respectively.These results indicate that the asymmetric electron-donating segments are promising to construct A-D-A type small molecular acceptors,which could largely enhance the diversity of building blocks to design photovoltaic materials. | Wenchao Zhai Ailing Tang Bo Xiao Xiaochen Wang Fan Chen Erjun Zhou | 2018 | Science Bulletin2018，63，13： | 1 |
| 8 | Promoting near-infrared II fluorescence efficiency by blocking long-range energy migration显示文摘Generally,long wavelength absorbed near-infrared II(NIR-II)dyes have a low fluorescence efficiency in aggregate states for aggregate-caused quenching effect,simultaneously enhancing efficiency and extending absorption is a challenging issue for NIR-II dyes.Here,three benzo[1,2-c:4,5-c’]bis[1,2,5]thiadiazole(BBT)derivatives(TPA-BBT,FT-BBT,and BTBT-BBT)are used to clarify fluorescence quenching mechanisms.When the BBT derivatives are doped into a small molecule matrix,they show quite different fluorescence behaviors.Structuredistorted TPA-BBT displays fluorescence quenching originating from short-range exchange interaction,while FT-BBT and BTBT-BBT with a co-planar-conjugated backbone exhibit concentration-dependent quenching processes,namely changing from long-range dipole-dipole interaction to exchange interaction,which could be majorly ascribed to large spectral overlap between absorption and emission.By precisely tuning doping concentration,both FT-BBT and BTBT-BBT nanoparticles(NPs)present the optimal NIR-II fluorescence brightness at∼2.5 wt%doping concentration.The doped NPs have good biocompatibility and could be served as fluorescence contrast agents for vascular imaging with a high resolution under 980-nm laser excitation.Those paradigms evidence that molecular doping can promote fluorescence efficiency of long wavelength-absorbed NIR-II fluorophores via suppressing long-range energy migration. | Changjin Ou Lei An Ziqi Zhao Fan Gao Liangyu Zheng Chao Xu Kang Zhang Jinjun Shao Linghai Xie Xiaochen Dong | 2023 | Aggregate2023，4，3： | 0 |
| 9 | Abnormal Aggregation of Invasive Cancer Cells Induced by Collective Polarization and ECM-Mediated Mechanical Coupling in Coculture Systems显示文摘Studiesonpattern formation in coculturecell systemscan provide insights intomany physiological and pathologicalprocesses Here,we investigate how theextracellular matrix(ECM)may influence the patterning in coculture systems.Themodel coculture system we use is composed of highly motile invasivebreast cancer cells,initially mixed with inert nonmetastatic cells on a 2D substrate and covered with a Matrigel layer introduced to mimic ECM.We observe that the invasive cells exhibit persistent centripetal motion and yield abnormal aggregation,rather than random spreading,due to a“cllective pulling”effect resultingfrom ECM-mediated transmssion of active contractile forces generated bythe polarized migration of the invasive cells along the vertical direction.Themechanism we report may open a new window for the understanding of biological processes that involve multiple types of cells. | Xiaochen Wang Shaohua Chen Hanqing Nan Ruchuan Liu Yu Ding Kena Song Jianwei Shuai Qihui Fan Yu Zheng Fangu Ye Yang Jiao Liyu Liu | 2022 | Research2022，，2： | 0 |
| 10 | Abnormal Aggregation of Invasive Cancer Cells Induced by Collective Polarization and ECM-Mediated Mechanical Coupling in Coculture Systems显示文摘Studies on pattern formation in coculture cell systems can provide insights into many physiological and pathological processes.Here,we investigate how the extracellular matrix(ECM)may influence the patterning in coculture systems.The model coculture system we use is composed of highly motile invasive breast cancer cells,initially mixed with inert nonmetastatic cells on a 2D substrate and covered with a Matrigel layer introduced to mimic ECM.We observe that the invasive cells exhibit persistent centripetal motion and yield abnormal aggregation,rather than random spreading,due to a“collective pulling”effect resulting from ECM-mediated transmission of active contractile forces generated by the polarized migration of the invasive cells along the vertical direction.The mechanism we report may open a new window for the understanding of biological processes that involve multiple types of cells. | Xiaochen Wang Shaohua Chen Hanqing Nan Ruchuan Liu Yu Ding Kena Song Jianwei Shuai Qihui Fan Yu Zheng Fangfu Ye Yang Jiao Liyu Liu | 2021 | Research2021，，1： | 0 |
| 11 | Conserved gene arrangement in the mitochondrial genomes of barklouse families Stenopsocidae and Psocidae显示文摘Substantial variation in gene organization and arrangement has been reported for sequenced mitochondrial(mt) genomes from the suborders of the insect order Psocoptera. In this study we sequenced the complete mt genome of Stenopsocus immaculatus, the first representative of the family Stenopsocidae from the suborder Psocomorpha. Relative to the ancestral pattern, rearrangements of a protein-coding gene(nad3) and five t RNA genes(trnQ, trnC, trnN, trnS1, trnE) were found. This pattern was similar to that of two barklice from the family Psocidae, with the exception of the translocation of trnS1,trnE and trnI. Based on comparisons of pairwise breakpoint distances of gene rearrangements, gene number and chromosome number, it was concluded that mt genomes of Stenopsocidae and Psocidae share a relatively conserved pattern of gene rearrangements; mt genomes within the Psocomorpha have been generally stable over long evolutionary history; and mt gene rearrangement has been substantially faster in the booklice(suborder Troctomorpha) than in the barklice(suborders Trogiomorpha and Psocomorpha). It is speculated that the change of life history and persistence of unusual reproductive systems with maternal inheritance contributed to the contrasting rates in mt genome evolution between the barklice and booklice. | Xiaochen LIU Hu LI Yao CAI Fan SONG John-James WILSON Wanzhi CAI | 2017 | Frontiers of Agricultural Science and Engineering2017，4，3： | 0 |
| 12 | Analysis of Summertime Typical Pollution in Pearl River Delta Region——Numerical Simulation of Meteorological Field显示文摘Based on meteorological field and air quality observation data,air pollution process over Pearl River Delta( PRD) in August of 2012 was analyzed. There were five notable pollution processes in this month,in which four pollution processes were related to typhoon. By using meso-scale meteorological model WRF,meteorological characteristics of two pollution processes induced by tropical cyclone were contrasted and analyzed. Results showed that O3and PM10concentrations significantly increased in PRD region during Typhoon Haikui period( 7-8 August),and other pollutant concentrations were also high. However,O3and PM10concentrations did not significantly increase during Typhoon Kai-Tak period( 16-17 August). This kind of concentration difference between two processes was related to typhoon pathway,wind direction and velocity,atmospheric boundary layer height,vertical transport and horizontal divergence field. | Zhang Xiaochen Liu Yiming Deng Xuejiao Chen Pakwai Feng Yerong Fan Qi | 2014 | Meteorological and Environmental Research2014，5，4： | 0 |
| 13 | Collective cell behaviors manipulated by synthetic DNA nanostructures显示文摘Cellular collective motion in confluent epithelial monolayers is involved in many processes such as embryo development,carcinoma invasion,and wound healing.The development of new chemical strategies to achieve largescale control of cells’collective motion is essential for biomedical applications.Here a series of DNA nanostructures with different dimensions were synthesized and their influences on cells’collective migration and packing behaviors in epithelial monolayers were investigated.We found that the framed DNA nanoassemblies effectively reduced the cells’speed by increasing the rigidity of cells,while the lipid-DNA micelles had a more pronounced effect on cells’projection area and shape factor.These DNA nanostructures all significantly enhanced the dependence of cells’speed on their shape factor.Our results indicate that cells’mobility in monolayers can be manipulated by chemical intercellular interactions without any genetic intervention.This may provide a new chemical strategy for tissue engineering and tumor therapy. | Xiaochen Wang Xiwen Xing Shuang Lu Guangle Du Yi Zhang Yubin RenYulong Sun Jing Sun Qihui Fan Kai Liu Fan Wang Fangfu Ye | 2023 | Fundamental Research2023，3，5： | 0 |
| 14 | Mettl13 protects against cardiac contractile dysfunction by negatively regulating C-Cbl-mediated ubiquitination of SERCA2a in ischemic heart failure显示文摘Ischemic heart failure(HF)remains a leading cause of morbidity and mortality.Maintaining homeostasis of cardiac function and preventing cardiac remodeling deterioration are critical to halting HF progression.Methyltransferase-like protein 13(Mettl13)has been shown to regulate protein translation efficiency by acting as a protein lysine methyltransferase,but its role in cardiac pathology remains unexplored.This study aims to characterize the roles and mechanisms of Mettl13 in cardiac contractile function and HF.We found that Mettl13 was downregulated in the failing hearts of mice post-myocardial infarction(MI)and in a cellular model of oxidative stress.Cardiomyocyte-specific overexpression of Mettl13 mediated by AAV9-Mettl13 attenuated cardiac contractile dysfunction and fibrosis in response to MI,while silencing of Mettl13 impaired cardiac function in normal mice.Moreover,Mettl13 overexpression abrogated the reduction in cell shortening,Ca^(2+)transient amplitude and SERCA2a protein levels in the cardiomyocytes of adult mice with MI.Conversely,knockdown of Mettl13 impaired the contractility of cardiomyocytes,and decreased Ca^(2+)transient amplitude and SERCA2a protein expression in vivo and in vitro.Mechanistically,Mettl13 impaired the stability of c-Cbl by inducing lysine methylation of c-Cbl,which in turn inhibited ubiquitination-dependent degradation of SERCA2a.Furthermore,the inhibitory effects of knocking down Mettl13 on SERCA2a protein expression and Ca^(2+)transients were partially rescued by silencing c-Cbl in H_(2)O_(2)-treated cardiomyocytes.In conclusion,our study uncovers a novel mechanism that involves the Mettl13/c-Cbl/SERCA2a axis in regulating cardiac contractile function and remodeling,and identifies Mettl13 as a novel therapeutic target for ischemic HF. | Shuting Yu ZhiYong Sun Xiuzhu Wang Tiantian Ju Changhao Wang Yingqi Liu Zhezhe Qu KuiWu Liu Zhongting Mei Na Li Meixi Lu Fan Wu Min Huang Xiaochen Pang Yingqiong Jia Ying Li Yaozhi Zhang Shunkang Dou Jianhao Jiang Xin Li Baofeng Yang Weijie Du | 2023 | Science China(Life Sciences)2023，66，12： | 0 |
| 15 | A Stellar Ranging Scheme Based on the Second-order Correlation Measurement显示文摘Stellar ranging is the basis for stellar dynamics research and in-depth research on astrophysics. The parallax method is the most widely used and important basic method for stellar ranging. However, it needs to perform highprecision measurement of the parallax angle and the baseline length together. We aim to propose a new stellar ranging scheme based on second-order correlation that does not require a parallax angle measurement. We hope our solution can be as basic as the parallax method. We propose a new stellar ranging scheme by using the offset of second-order correlation curve signals. The optical path difference between the stars and different base stations is determined by the offset of the second-order correlation curve signals. Then the distance of the stars could be determined by the geometric relation. With the distance to stars out to 10 kpc away, our astrometric precision can be better compared to Gaia by simulation. We also design an experiment and successfully demonstrate the feasibility of this scheme. This stellar ranging scheme enables further and more accurate stellar ranging without requiring any prior information or angle measurement. | Jian Li Can Xu Yinshen Liu Yaqi Ma Xinyao Liu Xiaochen Ma Run Fan An-Ning Zhang | 2022 | Research in Astronomy and Astrophysics2022，22，1： | 0 |
| 16 | Users'experience matter:Delay sensitivity-aware computation offloading in mobile edge computing显示文摘As a promising computing paradigm,Mobile Edge Computing(MEC)provides communication and computing capability at the edge of the network to address the concerns of massive computation requirements,constrained battery capacity and limited bandwidth of the Internet of Things(IoT)systems.Most existing works on mobile edge task ignores the delay sensitivities,which may lead to the degraded utility of computation offloading and dissatisfied users.In this paper,we study the delay sensitivity-aware computation offloading by jointly considering both user's tolerance towards delay of task execution and the network status under computation and communication constraints.Specifically,we use a specific multi-user and multi-server MEC system to define the latency sensitivity of task offloading based on the analysis of delay distribution of task categories.Then,we propose a scoring mechanism to evaluate the sensitivity-dependent utility of task execution and devise a Centralized Iterative Redirection Offloading(CIRO)algorithm to collect all information in the MEC system.By starting with an initial offloading strategy,the CIRO algorithm enables IoT devices to cooperate and iteratively redirect task offloading decisions to optimize the offloading strategy until it converges.Extensive simulation results show that our method can significantly improve the utility of computation offloading in MEC systems and has lower time complexity than existing algorithms. | Mingzhi Wang Tao Wu Tao Ma Xiaochen Fan Mingxing Ke | 2022 | Digital Communications and Networks2022，8，6： | 0 |
| 17 | Small Molecular NIR-II Fluorophores for Cancer Phototheranostics显示文摘Phototheranostics integrates deep-tissue imaging with phototherapy(containing photothermal therapy and photodynamic therapy),holding great promise in early diagnosis and precision treatment of cancers.Recently,second near-infrared(NIR-II)fluorescence imaging exhibits the merits of high accuracy and specificity,as well as real-time detection.Among the NIR-II fluorophores,organic small molecular fluorophores have shown superior properties in the biocompatibility,variable structure,and tunable emission wavelength than the inorganic NIR-II materials.What’s more,some small molecular fluorophores also display excellent cytotoxicity when illuminated with the NIR laser.This review summarizes the progress of small molecular NIR-II fluorophores with different central cores for cancer phototheranostics in the past few years,focusing on the molecular structures and phototheranostic performances.Furthermore,challenges and prospects of future development toward clinical translation are discussed. | Hanming Dai Qing Shen Jinjun Shao Wenjun Wang Fan Gao Xiaochen Dong | 2021 | The Innovation2021，2，1： | 0 |
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