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| 1 | Immobilization of insulin-like growth factor-1 onto thermosensitive hydrogels to enhance cardiac progenitor cell survival and differentiation under ischemic conditions显示文摘Stem cell therapy is a promising approach to treat myocardial infarction. However, direct delivery of stem cells into hearts experiences poor cell engraftment and differentiation, due to ischemic conditions(low nutrient and oxygen) in the infarct hearts. Development of suitable cell carriers capable of supporting cell survival and differentiation under these harsh conditions is critical for improving the efficacy of current stem cell therapy. In this work, we created a family of novel cell carriers based on thermosensitive hydrogels and insulin-like growth factor 1(IGF-1), and investigated if these cell carriers can improve cell survival and differentiation under ischemic conditions. The thermosensitive hydrogels were synthesized from N-isopropylacryla- mide, acrylic acid, acrylic acid N-hydroxysuccinicimide ester, and 2-hydroxyethyl methacrylate-oligo(hydroxybutyrate). The hydrogel solutions can be readily injected through 26G needles, and can quickly solidify at 37 oC to form highly flexible hydrogels. IGF-1 was immobilized into the hydrogels in order to support long-term cell survival and differentiation. Different amount of IGF-1 was immobilized by using hydrogels with different content of N-hydroxysuccinicimide ester groups. Cardiosphere derived cells were encapsulated in the hydrogels and cultured under ischemic conditions. The results demonstrated that a significant improvement of cell survival and differentiation was achieved after IGF-1 immobilization. These IGF-1 immobilized hydrogels have the potential to improve cell survival and differentiation in infarct hearts. | LI ZhenQing XU YanYi LI HaiChang GUAN JianJun | 2014 | Science China Chemistry2014，57，4： | 3 |
| 2 | Host HDAC4 regulates the antiviral response by inhibiting the phosphorylation of IRF3显示文摘Class II HDACs, such as HDAC4, are critical regulators of the immune response in various immune cells;however, its role in innate immunity remains largely unknown.Here, we report that the overexpression of HDAC4 suppresses the production of type I interferons triggered by pattern-recognition receptors (PRRs). HDAC4 repressed the translocation of transcription factor IRF3 to the nucleus, thereby decreasing IRF3-mediated IFN-β expression. In particular, we also determined that HDAC4 can be phosphorylated and simultaneously block the phosphorylation of IRF3 at Ser386 and Ser396 by TBK1 and IKKε, respectively, by interacting with the kinase domain of TBK1 and IKKε. Furthermore, IFN-β may stimulate the expression of HDAC4. Our findings suggest that HDAC4 acts as a regulator of PRR signaling and is a novel mechanism of negative feedback regulation for preventing an overreactive innate immune response. | Qi Yang Jielin Tang Rongjuan Pei XiaoXiao Gao Jing Guo Chonghui Xu Yun Wang QianWang Chunchen Wu Yuan Zhou Xue Hu He Zhao Yanyi Wang Xinwen Chen Jizheng Chen | 2019 | Journal of Molecular Cell Biology2019，11，2： | 2 |
| 3 | Thermosensitive and Highly Flexible Hydrogels Capable of Stimulating Cardiac Differentiation of Cardiosphere-Derived Cells under Static and Dynamic Mechanical Training Conditions显示文摘 | Li Zhenqing Fan Zhaobo Xu Yanyi | 2016 | Acs Applied Materials&Interfaces2016，85，15： | 1 |
| 4 | Highly sensitive fiber Bragg grating refractive index sensors显示文摘 | LIANG Wei HUANG Yanyi XU Yong | 2005 | Applied Physics Letters2005，86，15： | 1 |
| 5 | Nucleic acids analysis显示文摘Nucleic acids are natural biopolymers of nucleotides that store, encode, transmit and express genetic information, which play central roles in diverse cellular events and diseases in living things. The analysis of nucleic acids and nucleic acids-based analysis have been widely applied in biological studies, clinical diagnosis, environmental analysis, food safety and forensic analysis.During the past decades, the field of nucleic acids analysis has been rapidly advancing with many technological breakthroughs.In this review, we focus on the methods developed for analyzing nucleic acids, nucleic acids-based analysis, device for nucleic acids analysis, and applications of nucleic acids analysis. The representative strategies for the development of new nucleic acids analysis in this field are summarized, and key advantages and possible limitations are discussed. Finally, a brief perspective on existing challenges and further research development is provided. | Yongxi Zhao Xiaolei Zuo Qian Li Feng Chen Yan-Ru Chen Jinqi Deng Da Han Changlong Hao Fujian Huang Yanyi Huang Guoliang Ke Hua Kuang Fan Li Jiang Li Min Li Na Li Zhenyu Lin Dingbin Liu Juewen Liu Libing Liu Xiaoguo Liu Chunhua Lu Fang Luo Xiuhai Mao Jiashu Sun Bo Tang Fei Wang Jianbin Wang Lihua Wang Shu Wang Lingling Wu Zai-Sheng Wu Fan Xia Chuanlai Xu Yang Yang Bi-Feng Yuan Quan Yuan Chao Zhang Zhi Zhu Chaoyong Yang Xiao-Bing Zhang Huanghao Yang Weihong Tan Chunhai Fan | 2021 | Science China Chemistry2021，64，2： | 1 |
| 6 | Biomaterial property-controlled stem cell fates for cardiac regeneration显示文摘Myocardial infarction(MI)affects more than 8 million people in the United States alone.Due to the insufficient regeneration capacity of the native myocardium,one widely studied approach is cardiac tissue engineering,in which cells are delivered with or without biomaterials and/or regulatory factors to fully regenerate the cardiac functions.Specifically,in vitro cardiac tissue engineering focuses on using biomaterials as a reservoir for cells to attach,as well as a carrier of various regulatory factors such as growth factors and peptides,providing high cell retention and a proper microenvironment for cells to migrate,grow and differentiate within the scaffolds before implantation.Many studies have shown that the full establishment of a functional cardiac tissue in vitro requires synergistic actions between the seeded cells,the tissue culture condition,and the biochemical and biophysical environment provided by the biomaterials-based scaffolds.Proper electrical stimulation and mechanical stretch during the in vitro culture can induce the ordered orientation and differentiation of the seeded cells.On the other hand,the various scaffolds biochemical and biophysical properties such as polymer composition,ligand concentration,biodegradability,scaffold topography and mechanical properties can also have a significant effect on the cellular processes. | Yanyi Xu Jianjun Guan | 2016 | Bioactive Materials2016，1，1： | 0 |
| 7 | Chemical approaches for mimicking logic functions within fluorescent MPT dyes显示文摘The progress of the design, synthesis, fluorescence properties and application of a new family of fluorescent molecular switches towards information processing at the molecular level was reviewed. On the basis of the high fluorescence quantum yields and surroundings-sensitive fluorescent properties of the 5-methoxy-2-(2-pyridyl)-thiazole (2-MPT, 1) and a series of its derivatives as prepared, multiple binary logic and arithmetic functionalities were realized through encoding the controllable fluorescence switching properties with binary digit. Combined with the microfluidic platform, the fabrication of the molecular logic devices was attempted. | XU ChunHu SUN Wei ZHANG Chao BAI YanChun FANG ChenJie LI WenTao HUANG YanYi YAN ChunHua | 2009 | Science China Chemistry2009，52，6： | 0 |
| 8 | Multi-omics for COVID-19:driving development of therapeutics and vaccines显示文摘The ongoing COVID-19 pandemic caused by SARS-CoV-2 has raised global concern for public health and economy.The development of therapeutics and vaccines to combat this virus is continuously progressing.Multi-omics approaches,including genomics,transcriptomics,proteomics,metabolomics,epigenomics and metallomics,have helped understand the structural and molecular features of the virus,thereby assisting in the design of potential therapeutics and accelerating vaccine development for COVID-19.Here,we provide an up-to-date overview of the latest applications of multi-omics technologies in strategies addressing COVID-19,in order to provide suggestions towards the development of highly effective knowledge-based therapeutics and vaccines. | Mengyu Guo Muya Xiong Jinying Peng Tong Guan Haixia Su Yanyi Huang Cai-Guang Yang Yang Li Diana Boraschi Thanigaimalai Pillaiyar Guanbo Wang Chengqi Yi Yechun Xu Chunying Chen | 2023 | National Science Review2023，10，9： | 0 |
| 9 | The bio-distribution,clearance pathways,and toxicity mechanisms of ambient ultrafine particles显示文摘Ambient particles severely threaten human health worldwide.Compared to larger particles,ultrafine particles(UFPs)are highly concentrated in ambient environments,have a larger specific surface area,and are retained for a longer time in the lung.Recent studies have found that they can be transported into various extra-pulmonary organs by crossing the air-blood barrier(ABB).Therefore,to understand the adverse effects of UFPs,it is crucial to thoroughly investigate their bio-distribution and clearance pathways in vivo after inhalation,as well as their toxicological mechanisms.This review highlights emerging evidence on the bio-distribution of UFPs in pulmonary and extra-pulmonary organs.It explores how UFPs penetrate the ABB,the blood-brain barrier(BBB),and the placental barrier(PB)and subsequently undergo clearance by the liver,kidney,or intestine.In addition,the potential underlying toxicological mechanisms of UFPs are summarized,providing fundamental insights into how UFPs induce adverse health effects. | Dongyang Han Renjie Chen Haidong Kan Yanyi Xu | 2023 | Eco-Environment & Health2023，2，3： | 0 |
| 10 | Indoor metabolites and chemicals outperform microbiome in classifying childhood asthma and allergic rhinitis显示文摘Indoor microorganisms impact asthma and allergic rhinitis(AR),but the associated microbial taxa often vary extensively due to climate and geographical variations.To provide more consistent environmental assessments,new perspectives on microbial exposure for asthma and AR are needed.Home dust from 97 cases(32 asthma alone,37 AR alone,28 comorbidity)and 52 age-and gender-matched controls in Shanghai,China,were analyzed using high-throughput shotgun metagenomic sequencing and liquid chromatography-mass spectrometry.Homes of healthy children were enriched with environmental microbes,including Paracoccus,Pseudomonas,and Psychrobacter,and metabolites like keto acids,indoles,pyridines,and flavonoids(astragalin,hesperidin)(False Discovery Rate<0.05).A neural network co-occurrence probability analysis revealed that environmental microorganisms were involved in producing these keto acids,indoles,and pyridines.Conversely,homes of diseased children were enriched with mycotoxins and synthetic chemicals,including herbicides,insecticides,and food/cosmetic additives.Using a random forest model,characteristic metabolites and microorganisms in Shanghai homes were used to classify high and low prevalence of asthma/AR in an independent dataset in Malaysian schools(N=1290).Indoor metabolites achieved an average accuracy of 74.9%and 77.1%in differentiating schools with high and low prevalence of asthma and AR,respectively,whereas indoor microorganisms only achieved 51.0%and 59.5%,respectively.These results suggest that indoor metabolites and chemicals rather than indoor microbiome are potentially superior environmental indicators for childhood asthma and AR.This study extends the traditional risk assessment focusing on allergens or air pollutants in childhood asthma and AR,thereby revealing potential novel intervention strategies for these diseases. | Yu Sun Hao Tang Shuang Du Yang Chen Zheyuan Ou Mei Zhang Zhuoru Chen Zhiwei Tang Dongjun Zhang Tianyi Chen Yanyi Xu Jiufeng Li Dan Norback Jamal Hisham Hashim Zailina Hashim Jie Shao Xi Fu Zhuohui Zhao | 2023 | Eco-Environment & Health2023，2，4： | 0 |
| 11 | Histone deacetylase 4 inhibits NF-κB activation by facilitating IκBα sumoylation显示文摘Protein modification by small ubiquitin-like modifier(SUMO)is an important regulatory mechanism for multiple cellular pro-cesses.Although the canonical pathway involving the ubiquitylation or phosphorylation of IκBα has been well characterized,little is known about the sumoylation of IkBa in the control of NF-κB activity.Here,we find that histone deacetylase 4(HDAC4)negatively regulates tumor necrosis factor-alpha-or lipopolysaccharide-triggered NF-κB activation.HDAC4 belongs to the SUMO E3 ligase family and can directly sumoylate IκBα.The cytoplasm location of HDAC4 is essential for IκBαsumoylation.The Cys292 of HDAC4 is a key site for its SUMO E3 ligase activity.The sumoylation of IkBc prevents its polyubiquitination and degradation be-cause these two modifications occur both at the Lys21.Our findings reveal a previously undiscovered role for HDAC4 in the inflammatory response as a SUMO E3 ligase for IκBαsumoylation.Our work provides insight into mechanisms ensuring optimal mediation of the NF-κB pathway. | Qi Yang Jielin Tang Chonghui Xu He Zhao Yuan Zhou Yanyi Wang Min Yang Xinwen Chen Jizheng Chen | 2020 | Journal of Molecular Cell Biology2020，12，12： | 0 |
| 12 | 人工智能白内障协同管理的通用平台显示文摘目的:建立和验证一个涉及多级临床场景的白内障协作通用的人工智能(artificial intelligence,AI)管理平台,探索基于AI的医疗转诊模式,以提高协作效率和资源覆盖率。方法:训练和验证的数据集来自中国AI医学联盟,涵盖多级医疗机构和采集模式。使用三步策略对数据集进行标记:1)识别采集模式;2)白内障诊断包括正常晶体眼、白内障眼或白内障术后眼;3)从病因和严重程度检测需转诊的白内障患者。此外,将白内障AI系统与真实世界中的居家自我监测、初级医疗保健机构和专科医院等多级转诊模式相结合。结果:通用AI平台和多级协作模式在三步任务中表现出可靠的诊断性能:1)识别采集模式的受试者操作特征(receiver operating characteristic curve,ROC)曲线下面积(area under the curve,AUC)为99.28%~99.71%);2)白内障诊断对正常晶体眼、白内障或术后眼,在散瞳-裂隙灯模式下的AUC分别为99.82%、99.96%和99.93%,其他采集模式的AUC均>99%;3)需转诊白内障的检测(在所有测试中AUC>91%)。在真实世界的三级转诊模式中,该系统建议30.3%的人转诊,与传统模式相比,眼科医生与人群服务比率大幅提高了10.2倍。结论:通用AI平台和多级协作模式显示了准确的白内障诊断性能和有效的白内障转诊服务。建议AI的医疗转诊模式扩展应用到其他常见疾病和资源密集型情景当中。 | WU Xiaohang HUANG Yelin LIU Zhenzhen LAI Weiyi LONG Erping ZHANG Kai JIANG Jiewei LIN Duoru CHEN Kexin YU Tongyong WU Dongxuan LI Cong CHEN Yanyi ZOU Minjie CHEN Chuan ZHU Yi GUO Chong ZHANG Xiayin WANG Ruixin YANG Yahan XIANG Yifan CHEN Lijian LIU Congxin XIONG Jianhao GE Zongyuan WANG Dingding XU Guihua DU Shaolin XIAO Chi WU Jianghao ZHU Ke NIE Danyao XU Fan LV Jian CHEN Weirong LIU Yizhi 林浩添 无 王厚硕(审校) 罗明杰(审校) | 2023 | 眼科学报2023，38，10： | 0 |
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