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| 1 | Cold-chain food contamination as the possible origin of COVID-19 resurgence in Beijing显示文摘COVID-19,caused by SARS-CoV-2[1,2],has been contained in China through stringent non-pharmaceutical interventions.Border control and quarantine have effectively prevented the virus from being spread by infected travellers,but the risk of resurgence caused by other routes of introduction and transmission remains unclear,and current strategies to prevent resurgence could be flawed.Since July,SARS-CoV-2 RNA contaminations in frozen food imported from countries with ongoing epidemics have been reported in nine provinces in China[3,4].However,there is no robust evidence of COVID-19 outbreaks initiated by environmentto-human transmission.Here we add to evidence of such transmission by investigating the recent COVID-19 resurgence in Beijing. | Xinghuo Pang Lili Ren Shuangsheng Wu Wentai Ma Jian Yang Lin Di Jie Li Yan Xiao Lu Kang Shichang Du Jing Du Jing Wang Gang Li Shuguang Zhai Lijuan Chen Wenxiong Zhou Shengjie Lai Lei Gao Yang Pan Quanyi Wang Mingkun Li Jianbin Wang Yanyi Huang Jianwei Wang COVID-19 Field Response Group COVID-19 Laboratory Testing Group | 2020 | National Science Review2020，7，12： | 51 |
| 2 | Single-cell triple omics sequencing reveals genetic, epigenetic, and transcriptomic heterogeneity in hepatocellular carcinomas显示文摘 | Yu Hou Huahu Guo then Cao Xianlong Li Boqiang Hu Ping Zhu Xinglong Wu Lu Wen FuchouTang Yanyi Huang Jirun Peng | 2016 | Cell Research2016，26，3： | 48 |
| 3 | Genome-scale detection of hypermethylated CpG islands in circulating cell-free DNA of hepatocellular carcinoma patients显示文摘 | Lu Wen Jingyi Li Huahu Guo Xiaomeng Liu Shengmin Zheng Dafang Zhang Weihua Zhu Jianhui Qu Limin Guo Dexiao Du Xiao Jin Yuhao Zhang Yun Gao Jie Shen Hao Ge Fuchou Tang Yanyi Huang Jirun Peng | 2015 | Cell Research2015，25，11： | 19 |
| 4 | Distribution of heavy metals in sediments of the Pearl River Estuary,Southern China:Implications for sources and historical changes显示文摘The distribution of heavy metals(Pb,Zn,Cd and As) in sediments of the Pearl River Estuary was investigated.The spatial distribution of heavy metals displayed a decreasing pattern from the turbidity maxima to both upstream and downstream of the estuary,which suggested that suspended sediments played an important role in the trace metal distribution in the Pearl River Estuary.In addition,metal concentrations were higher in the west part of the estuary which received most of the pollutants from the Pearl River.In the sediment cores,fluxes of heavy metals were consistent with a predominant anthropogenic input in the period 1970-1990.From the mid-1990s to the 2000s,there was a significant decline in heavy metal pollution.The observed decline has shown the result of pollution control in the Pearl River Delta.However,it is noteworthy that the metal concentrations in the most recent sediment still remained considerably high.Taken together,the enrichment of heavy metals in sediments was largely controlled by anthropogenic pollution. | Feng Ye Xiaoping Huang Dawen Zhang Lei Tian Yanyi Zeng | 2012 | Journal of Environmental Sciences2012，24，4： | 18 |
| 5 | H3K4me3 epigenomic landscape derived from ChIP-Seq of 1000 mouse early embryonic cells显示文摘 | Jie Shen Dongqing Jiang Yusi Fu Xinglong Wu Hongshan Guo Binxiao Feng Yuhong Pang Aaron M Streets Fuchou Tang Yanyi Huang | 2015 | Cell Research2015，25，1： | 4 |
| 6 | Copy number alteration profiling facilitates differential diagnosis between ossifying fibroma and fibrous dysplasia of the jaws显示文摘Ossifying fibroma(OF)and fibrous dysplasia(FD)are two fibro-osseous lesions with overlapping clinicopathological features,making diagnosis challenging.In this study,we applied a whole-genome shallow sequencing approach to facilitate differential diagnosis via precise profiling of copy number alterations(CNAs)using minute amounts of DNA extracted from morphologically correlated microdissected tissue samples.Freshly frozen tissue specimens from OF(n=29)and FD(n=28)patients were obtained for analysis.Lesion fibrous tissues and surrounding normal tissues were obtained by laser capture microdissection(LCM),with~30–50 cells(5000–10000µm2)per sample.We found that the rate of recurrent CNAs in OF cases was much higher(44.8%,13 of 29)than that in FD cases(3.6%,1 of 28).Sixty-nine percent(9 of 13)of the CNA-containing OF cases involved segmental amplifications and deletions on Chrs 7 and 12.We also identified eight CNA-associated genes(HILPDA,CALD1,C1GALT1,MICALL2,PHF14,AIMP2,MDM2,and CDK4)with amplified expression,which was consistent with the copy number changes.We further confirmed a jaw lesion with a previous uncertain diagnosis due to its ambiguous morphological features and the absence of GNAS mutation as OF based on the typical Chr 12 amplification pattern in its CNA profile.Moreover,analysis of a set of longitudinal samples collected from an individual with a cellular lesion in suspicion of OF at the first surgery,recurrence and the latest malignant transformation revealed identical CNA patterns at the three time points,suggesting that copy number profiling can be used as an important tool to identify borderline lesions or lesions with malignant potential.Overall,CNA profiling of fibro-osseous lesions can greatly improve differential diagnosis between OF and FD and help predict disease progression. | Ming Ma Lu Liu Ruirui Shi Jianyun Zhang Xiaotian Li Xuefen Li Jiaying Bai Jianbin Wang Yanyi Huang Tiejun Li | 2021 | International Journal of Oral Science2021，13，2： | 3 |
| 7 | Improvement in the risk assessment of oral leukoplakia through morphology-related copy number analysis显示文摘Oral leukoplakia is the most common type of oral potentially malignant disorders and considered a precursor lesion to oral squamous cell carcinoma.However,a predictor of oral leukoplakia prognosis has not yet been identified.We investigated whether copy number alteration patterns may effectively predict the prognostic outcomes of oral leukoplakia using routinely processed paraffin sections.Comparison of copy number alteration patterns between oral leukoplakia with hyperplasia(HOL,n=22)and dysplasia(DOL,n=21)showed that oral leukoplakia with dysplasia had a higher copy number alteration rate(86%)than oral leukoplakia with hyperplasia(46%).Oral leukoplakia with dysplasia exhibited a wider range of genomic variations across all chromosomes compared with oral leukoplakia with hyperplasia.We also examined a retrospective cohort of 477 patients with oral leukoplakia with hyperplasia with detailed follow-up information.The malignant transformation(MT,n=19)and leukoplakia recurrence(LR,n=253)groups had higher frequencies of aneuploidy events and copy number loss rate than the free of disease(FD,n=205)group.Together,our results revealed the association between the degree of copy number alterations and the histological grade of oral leukoplakia and demonstrated that copy number alteration may be effective for prognosis prediction in oral leukoplakia patients with hyperplasia. | Xiaotian Li Lu Liu Jianyun Zhang Ming Ma Lisha Sun Xuefen Li Heyu Zhang Jianbin Wang Yanyi Huang Tiejun Li | 2021 | Science China(Life Sciences)2021，64，9： | 2 |
| 8 | An integrated microfluidic device for long-term culture of isolated single mammalian cells显示文摘We developed an integrated microfluidic chip for long-term culture of isolated single cells. This polydimethylsiloxane (PDMS) based device could accurately seed each single cell into different culture chambers, and isolate one chamber from each other with monolithically integrated pneumatic valves. We optimized the culture conditions, including the frequency of medium replacement and the introduction of conditioned medium, to keep the single cells alive for 4 days. We cultured a few hundred cells in a separated chamber on the same chip to continuously supply the conditioned medium into the culture chambers for single cells. This approach greatly facilitated the growth of single cells, and created a suitable microenvironment for observing cells' autonomous process in situ without the interference of other adjacent cells. This single cell colony assay is expandable to higher throughput, fitting the needs in the studies of drug screening and stem cell differentiation. | ZHENG ChunHong CHEN Gui’E PANG YuHong HUANG YanYi | 2012 | Science China Chemistry2012，55，4： | 2 |
| 9 | Photoelectric behavior of nanocrystalline TiO2 electrode with a novel terpyridyl ruthenium complex显示文摘 | Wang Zhongsheng Huang Chunhui Huang Yanyi | 2002 | Solar Energy Materials & Solar Cells2002，71，2： | 1 |
| 10 | A highly efficient solar cell made from a dye-modified ZnO-covered TiO2 nanoporous electrode 显示文摘 | WANG Zhongsheng HUANG Chunhui HUANG Yanyi | 2001 | Chemistry of Materials2001，13，2： | 1 |
| 11 | A water-free metal organic deposition method for YBa2Cu3O7?δ thin film fabrication显示文摘 | Rongxia Huang Feng Feng Wei Wu Yunran Xue Yanyi Zhang Kai Shi Timing Qu Yongjie Zhao Xiaohao Wang Xiaowen Zhang Zhenghe Han | 2013 | Superconductor Science and Technology2013，，： | 1 |
| 12 | Highly sensitive fiber Bragg grating refractive index sensors显示文摘 | LIANG Wei HUANG Yanyi XU Yong | 2005 | Applied Physics Letters2005，86，15： | 1 |
| 13 | Statistical analysis of accumulation patterns of heavy metals in biota from the Pearl River Estuary, South China显示文摘 | ZENG Yanyi HUANG Xiaoping YE Feng | 2012 | Aquatic Ecosystem Health & Management2012，15，2： | 1 |
| 14 | 显示文摘 | Xin Hao Li Fuyou Huang Yanyi | 2002 | Journal of Rare Earths2002，20，5： | 1 |
| 15 | Nucleic acids analysis显示文摘Nucleic acids are natural biopolymers of nucleotides that store, encode, transmit and express genetic information, which play central roles in diverse cellular events and diseases in living things. The analysis of nucleic acids and nucleic acids-based analysis have been widely applied in biological studies, clinical diagnosis, environmental analysis, food safety and forensic analysis.During the past decades, the field of nucleic acids analysis has been rapidly advancing with many technological breakthroughs.In this review, we focus on the methods developed for analyzing nucleic acids, nucleic acids-based analysis, device for nucleic acids analysis, and applications of nucleic acids analysis. The representative strategies for the development of new nucleic acids analysis in this field are summarized, and key advantages and possible limitations are discussed. Finally, a brief perspective on existing challenges and further research development is provided. | Yongxi Zhao Xiaolei Zuo Qian Li Feng Chen Yan-Ru Chen Jinqi Deng Da Han Changlong Hao Fujian Huang Yanyi Huang Guoliang Ke Hua Kuang Fan Li Jiang Li Min Li Na Li Zhenyu Lin Dingbin Liu Juewen Liu Libing Liu Xiaoguo Liu Chunhua Lu Fang Luo Xiuhai Mao Jiashu Sun Bo Tang Fei Wang Jianbin Wang Lihua Wang Shu Wang Lingling Wu Zai-Sheng Wu Fan Xia Chuanlai Xu Yang Yang Bi-Feng Yuan Quan Yuan Chao Zhang Zhi Zhu Chaoyong Yang Xiao-Bing Zhang Huanghao Yang Weihong Tan Chunhai Fan | 2021 | Science China Chemistry2021，64，2： | 1 |
| 16 | Spiro-oxazine pho-tochromic fiber optical switch显示文摘 | HUANG Yanyi LIANG Wei POON J K S | 2006 | Applied Physics Letters2006，8，8： | 1 |
| 17 | Highly sensitive fiber Bragg grating refractive index sensors显示文摘 | Wet Liang Yanyi Huang Amnon Yariv | 2005 | Applied Physics Letters2005，86，5： | 1 |
| 18 | MR-seq:measuring a single cell's transcriptome repeatedly by RNA-seq显示文摘We described a novel single-cell RNA-seq technique called MR-seq(measure a single-cell transcriptome repeatedly),which permits statistically assessing the technical variation and identifying the differentially expressed genes between just two single cells by measuring each single cell twice. We demonstrated that MR-seq gave sensitivity and reproducibility similar to the standard single-cell RNA-seq and increased the positive predicate value. Application of MR-seq to early mouse embryos identified hundreds of candidate intra-embryonic heterogeneous genes among mouse 2-,4-and 8-cell stage embryos. MR-seq should be useful for detecting differentially expressed genes among a small number of cells. | Lu Yang Zhaochun Ma Chen Cao Yuhao Zhang Xinglong Wu Raymond Lee Boqiang Hu Lu Wen Hao Ge Yanyi Huang Kaiqin Lao Fuchou Tang | 2017 | Science Bulletin2017，62，6： | 1 |
| 19 | Chemical approaches for mimicking logic functions within fluorescent MPT dyes显示文摘The progress of the design, synthesis, fluorescence properties and application of a new family of fluorescent molecular switches towards information processing at the molecular level was reviewed. On the basis of the high fluorescence quantum yields and surroundings-sensitive fluorescent properties of the 5-methoxy-2-(2-pyridyl)-thiazole (2-MPT, 1) and a series of its derivatives as prepared, multiple binary logic and arithmetic functionalities were realized through encoding the controllable fluorescence switching properties with binary digit. Combined with the microfluidic platform, the fabrication of the molecular logic devices was attempted. | XU ChunHu SUN Wei ZHANG Chao BAI YanChun FANG ChenJie LI WenTao HUANG YanYi YAN ChunHua | 2009 | Science China Chemistry2009，52，6： | 0 |
| 20 | A virtual sequencer reveals the dephasing patterns in error-correction code DNA sequencing显示文摘An error-correction code(ECC)sequencing approach has recently been reported to effectively reduce sequencing errors by interrogating a DNA fragment with three orthogonal degenerate sequencing-by-synthesis(SBS)reactions.However,similar to other non-single-molecule SBS methods,the reaction will gradually lose its synchronization within a molecular colony in ECC sequencing.This phenomenon,called dephasing,causes sequencing error,and in ECC sequencing,induces distinctive dephasing patterns.To understand the characteristic dephasing patterns of the dual-base flowgram in ECC sequencing and to generate a correction algorithm,we built a virtual sequencer in silico.Starting from first principles and based on sequencing chemical reactions,we simulated ECC sequencing results,identified the key factors of dephasing in ECC sequencing chemistry and designed an effective dephasing algorithm.The results show that our dephasing algorithm is applicable to sequencing signals with at least 500 cycles,or1000-bp average read length,with acceptably low error rate for further parity checks and ECC deduction.Our virtual sequencer with our dephasing algorithm can further be extended to a dichromatic form of ECC sequencing,allowing for a potentially much more accurate sequencing approach. | Wenxiong Zhou Li Kang Haifeng Duan Shuo Qiao Louis Tao Zitian Chen Yanyi Huang | 2021 | National Science Review2021，8，5： | 0 |
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