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| 1 | Resequencing of 145 Landmark Cultivars Reveals Asymmetric Sub-genome Selection and Strong Founder Genotype Effects on Wheat Breeding in China显示文摘Controlled pedigrees and the multi-decade timescale of national crop plant breeding programs offer a unique experimental context for examining how selection affects plant genomes.More than 3000 wheat cultivars have been registered,released,and documented since 1949 in China.In this study,a set of 145 elite cultivars selected from historical points of wheat breeding in China were re-sequenced.A total of 43.75 Tb of sequence data were generated with an average read depth of 17.94x for each cultivar,and more than 60.92 million SNPs and 2.54 million InDels were captured,based on the Chinese Spring RefSeq genome v1.0.Seventy years of breeder-driven selection led to dramatic changes in grain yield and related phenotypes,with distinct genomic regions and phenotypes tar-geted by different breeders across the decades.There are very clear instances illustrating how introduced Italian and otherforeign germplasm was integrated into Chinese wheat programs and reshaped the genomic landscape of local modern cultivars.Importantly,the resequencing data also highlighted significant asymmetric breeding selec-tion among the three sub-genomes:this was evident in both the collinear blocks for homeologous chromosomes and among sets of three homeologous genes.Accumulation of more newly assembled genes in newer cultivars implied the potential value of these genes in breeding.Conserved and extended sharing of linkage disequilibrium(LD)blocks was highlighted among pedigree-related cultivars,in which fewer haplotype differences were detected.Fixation or replacement of haplotypes from founder genotypes after generations of breeding was related to their breeding value.Based on the haplotype frequency changes in LD blocks of pedigree-related cultivars,we propose a strategy for evaluating the breeding value of any given line on the basis of the accumulation(pyramiding)of bene-ficial haplotypes.Collectively,our study demonstrates the influence of 'founder genotypes' on the output of breeding efforts over many decades and also suggests that founder genotype perspectives are in fact more dy-namic when applied in the context of modern genomics-informed breeding. | Chenyang Hao Chengzhi Jiao Jian Hou Tian Li Hongxia Liu Yuquan Wang Jun Zheng Hong Liu Zhihong Bi Fengfeng Xu Jing Zhao Lin Ma Yamei Wang Uzma Majeed Xu Liu Rudi Appels Marco Maccaferri Roberto Tuberosa Hongfeng Lu Xueyong Zhang | 2020 | Molecular Plant2020，13，12： | 9 |
| 2 | MiR156 regulates anthocyanin biosynthesis through SPL targets and other microRNAs in poplar显示文摘Anthocyanins biosynthesized from the flavonoid pathway are types of pigments that are involved in the protection of poplar from biotic and abiotic stresses.Previous researchers studying anthocyanin-related transcription factors and structural genes in poplar have made significant discoveries.However,little is known about the regulatory role of microRNAs in anthocyanin biosynthesis in poplar.Here,we overexpressed miR156 in poplar to study the comprehensive effects of the miR156-SPL module on the biosynthesis of anthocyanins.Small RNA sequencing analysis revealed 228 microRNAs differentially expressed in transgenic poplar plants with dramatically increased miR156 levels.Furthermore,integrated microRNAomic and transcriptomic analysis suggested that two microRNAs,miR160h,and miR858,have the potential to affect anthocyanin accumulation in poplar by regulating auxin response factors and MYB transcription factors,respectively.Additionally,the accumulation of miR160h and miR858 displayed a positive correlation with miR156 levels,suggesting a possible interaction between the miR156-SPL module and these microRNAs in poplar.Last,metabolomics analysis revealed that the levels of anthocyanins,flavones,and flavonols were substantially elevated in transgenic poplar plants overexpressing miR156 compared with the wild type,whereas the total lignin content was reduced in the transgenic plants.Taken together,our results indicate that miR156 can fine tune the anthocyanin biosynthetic pathway via multiple factors,including microRNAs,transcription factors,and the levels of structural genes,in poplar.This provides additional clues for understanding the complex regulatory network of anthocyanin biosynthesis in woody plants. | Yamei Wang Wenwen Liu Xinwei Wang Ruijuan Yang Zhenying Wu HanWang LeiWang Zhubing Hu Siyi Guo Hailing Zhang Jinxing Lin Chunxiang Fu | 2020 | Horticulture Research2020，7，1： | 9 |
| 3 | Cranial irradiation impairs intrinsic excitability and synaptic plasticity of hippocampal CA1 pyramidal neurons with implications for cognitive function显示文摘Radiation therapy is a standard treatment for head and neck tumors.However,patients often exhibit cognitive impairments following radiation therapy.Previous studies have revealed that hippocampal dysfunction,specifically abnormal hippocampal neurogenesis or neuroinflammation,plays a key role in radiation-induced cognitive impairment.However,the long-term effects of radiation with respect to the electrophysiological adaptation of hippocampal neurons remain poorly characterized.We found that mice exhibited cognitive impairment 3 months after undergoing 10 minutes of cranial irradiation at a dose rate of 3 Gy/min.Furthermore,we observed a remarkable reduction in spike firing and excitatory synaptic input,as well as greatly enhanced inhibitory inputs,in hippocampal CA1 pyramidal neurons.Corresponding to the electrophysiological adaptation,we found reduced expression of synaptic plasticity marker VGLUT1 and increased expression of VGAT.Furthermore,in irradiated mice,long-term potentiation in the hippocampus was weakened and GluR1 expression was inhibited.These findings suggest that radiation can impair intrinsic excitability and synaptic plasticity in hippocampal CA1 pyramidal neurons. | Min-Yi Wu Wen-Jun Zou Pei Yu Yuhua Yang Shao-Jian Li Qiang Liu Jiatian Xie Si-Qi Chen Wei-Jye Lin Yamei Tang | 2022 | Neural Regeneration Research2022，17，10： | 5 |
| 4 | Verification of Mutagen Function of Zeocin in Nannochloropsis oceanica Through Transcriptome Analysis显示文摘Zeocin can cause double strand breaks of DNA and thus is frequently used as a selective antibiotic of eukaryotic Sh ble transformants. In non-transformation system, Zeocin may function as a mutagen if not totally lethal. To verify such function of Zeocin, we mutated Nannochloropsis oceanica by increasing the concentration of Zeocin in medium gradually, and isolated a N. oceanica strain(single cell culture) which survived Zeocin up to 10.0μg mL^(-1). The Zeocin-tolerant strain entered the exponential growth phase later and grew slower than the wild strain. Transcriptome profiling showed that the Zeocin-tolerant N. oceanica strain survived Zeocin mainly by adapting(heritable), rather than acclimating(plastic) to Zeocin. Hence mutating N. oceanica with Zeocin was approved effective. Meanwhile, the physiological characteristics of this Zeocin-tolerant strain were demonstrated. As we proposed, N. oceanica tolerated Zeocin by strengthening its protein degradation and antioxidation. The genes controlling cell division and cellular response to stimuli may also have played important roles in the reduction of growth and the tolerance to Zeocin. Our findings evidenced that Zeocin can serve as an appropriate mutagen of microalgae. Creating variations through mutation with Zeocin may help to study the genetic basis of the traits of this monoploidy and asexual microalga, as well as improve its production. | LIN Genmei WANG Yamei GUO Li DING Haiyan HU Yongmei LIANG Sijie ZHANG Zhongyi YANG Guanpin | 2017 | Journal of Ocean University of China2017，16，3： | 5 |
| 5 | The effects of different nitrogen sources on camptothecin content and related gene expression in Camptotheca acuminata seedlings显示文摘Camptotheac acuminata Decne is a unique tree species in China with an important secondary metabolite,camptothecin(CPT),used in the treatment of cancer.Nitrogen(N)is an important element that affects plant growth and the accumulation of CPT.Reports on the effect of N on CPT synthesis from a genetic perspective are scarce.To explore the effects of different N sources and levels on CPT synthesis in C.acuminata,two-year-old seedlings were fertilized with different concentrations of pure ammonium sulphate,source of ammonium N(NH4+-N),and potassium nitrate for nitrate N(NO3--N).Concentrations of 2.5,5,7.5,and10 g pot-1 NH4+-N and NO3--N were used.The results showed that 7.5 g NH4+-N and NO3--N treatments were best for growth and fresh weight of leaves.Compared with the other treatments,the CPT content,tryptophan synthase and tryptophan decarboxylase activities,and expression of the CaTSB and CaTDCl genes under the 2.5 g NH4+-N and NO3--N treatments peaked significantly at 30 days.However,the expression of CaTDC2 surpassed that of the other two genes at 60 days.Therefore,compared with NH4+-N source,the NO3--N source was more beneficial for growth,and NO3--N was better for CPT yield.Consequently,leaves of C.acuminata treated with 2.5 g NO3--N could be harvested after 30 days to obtain maximum CPT content.CaTDC1 is more closely linked to CPT synthesis.The results of this study improved the production of CPT in C.acuminata via fertilization. | Xiaode Wang Sainan Bian Pengjie Chang Ninghang Wang Lingjuan Xuan Mingru Zhang Bin Dong Chao Zhang Jiasheng Wu Yeqing Ying Xiazhen Lin Yamei Shen | 2020 | Journal of Forestry Research2020，31，4： | 3 |
| 6 | Multi-omic characterization of genome-wide abnormal DNA methylation reveals diagnostic and prognostic markers for esophageal squamous-cell carcinoma显示文摘This study investigates aberrant DNA methylations as potential diagnosis and prognosis markers for esophageal squamous-cell carcinoma(ESCC),which if diagnosed at advanced stages has<30%five-year survival rate.Comparing genome-wide methylation sites of 91 ESCC and matched adjacent normal tissues,we identified 35,577 differentially methylated CpG sites(DMCs)and characterized their distribution patterns. | Yiyi Xi Yuan Lin Wenjia Guo Xinyu Wang Hengqiang Zhao Chuanwang Miao Weiling Liu Yachen Liu Tianyuan Liu Yingying Luo Wenyi Fan Ai Lin Yamei Chen Yanxia Sun Yulin Ma Xiangjie Niu Ce Zhong Wen Tan Meng Zhou Jianzhong Su Chen Wu Dongxin Lin | 2022 | Signal Transduction and Targeted Therapy2022，7，3： | 2 |
| 7 | Carbohydrate-derived porous carbon materials:An ideal platform for green organic synthesis显示文摘Porous carbon materials have attracted much attention in the field of organic synthesis in recent years,due to their tunable properties,excellent catalytic activity and stability.Biomass-based carbohydrates emerge as an ideal precursor for the generation of these materials owing to their renewability,low cost,non-toxicity and high content of functional groups.Thus,carbon materials prepared from carbohydrates is of considerable importance for the sustainable development of organic chemistry.The present review not only summarizes recent examples of carbohydrate-derived porous carbon material-catalyzed organic reactions including the oxidation,hydrogenation,cross-coupling,esterification and condensation reactions,but also introduces the preparation and functionalization strategies of these materials.Furthermore,the challenges and opportunities of organic synthesis over these sustainable materials have also been addressed.This review will stimulate further research on exploring novel carbohydrate-derived porous carbon materials and new sustainable organic synthetic processes over these materials. | Yamei Lin Jie Yu Xing Zhang Jingkun Fang Guo-Ping Lu He Huang | 2022 | Chinese Chemical Letters2022，33，1： | 2 |
| 8 | Effects of sintering temperature on properties of porous mullite/corundum ceramic显示文摘 | Yang Fengkun Li Cuiwei Lin Yamei | | 0，，： | 1 |
| 9 | Single-atomic-site iron on N-doped carbon for chemoselective reduction of nitroarenes显示文摘A facile,gram-scale and sustainable approach has been established for the synthesis of single-atomic-site iron on N-doped carbon(Fe_(SA)@NC-20A)via the pyrolysis of aniline modified FeZn-ZIFs,in which the synthesis of zeolitic imidazolate frameworks(ZIFs)can be accomplished in water at room temperature,and no acid etching is required.The as-synthesized catalyst exhibits better performance on the chemoselective hydrogenation of nitroarenes with a broad substrate scope(turnover frequency(TOF)up to 1,727 h^(-1),23 examples)than most of previously reported works.Based on high-angle annular dark field scanning transmission microscopy(HAADF-STEM)images in combination with X-ray photoelectron spectroscopy(XPS),X-ray absorption spectroscopy(XAS),electron spin resonance(ESR),and Mossbauer spectroscopy,Fe is dispersed as single atoms via forming FeNx(x=4-6).This work not only determines the active sites of FesA@NC-20A for hydrogenation(FeN4),but also proposes tentative pathways for both N-H activation of hydrazine and the reduction of nitroarene on FeN4 site,both of which are the key steps for the hydrogenation of nitroarenes.In addition,this catalyst shows excellent stability,and no significant activity degradation is observed when recycling for 10 times or restoring in air for 2 months. | Guoping Lu Kangkang Sun Yamei Lin Qixuan Du Jiawei Zhang Kui Wang Pengcheng Wang | 2022 | Nano Research2022，15，1： | 1 |
| 10 | New Insights into the Dysfunctions of Pericytes and Neurovascular Units in Neurodegenerative Diseases显示文摘The neurovascular unit(NVU)is an essential anatomical and physiological entity for the maintenance of brain homeostasis and function;it is composed of endothelial cells,pericytes,astrocytes,neurons,vascular smooth muscle cells,microglia,and oligodendroglia[1].The NVU regulates important brain vessel properties such as the integrity of the blood-brain barrier(BBB)and cerebral blood flow(CBF).Considerable efforts have been made to investigate the cerebral microcirculatory dysfunction in ageing and Alzheimer’s disease(AD)[2]. | Qing Liu Wei-Jye Lin Yamei Tang | 2020 | Neuroscience Bulletin2020，36，12： | 1 |
| 11 | Lignin-derived Zn single atom/N-codoped porous carbon forα-alkylation of aromatic ketones with alcohols via borrowing hydrogen strategy显示文摘A green and scalable strategy has been developed for the synthesis of lignin-derived Zn single atom/N-codoped porous carbon(LCN@Zn-SAC)containing similar ZnNx sites with carbonic anhydrases.This catalyst exhibits superior activity on theα-alkylation of ketones with alcohols via borrowing hydrogen strategy(TON up to 15 h^(−1))than most of previously reported works.The dehydrogenation of benzyl alcohol is the rate-determining step based on kinetic experiment results.According to experimental and theoretical calculation results,Zn electron density is inversely proportional to reaction energy barriers,because Zn sites with less positive charge(ZnN_(4) and ZnN_(3)C)in Zn-SACs display better borrowing hydrogen ability than other Zn sites.Furthermore,this catalyst can be recycled by simple centrifugation,which can be reused at least 8 runs with no obvious lose in activity.To the best of our knowledge,this is the first example of non-noble metal-SAC-catalyzedα-alkylation via borrowing hydrogen strategy. | Xueping Zhang Guo-Ping Lu Kun Wang Yamei Lin Pengcheng Wang Wenbin Yi | 2022 | Nano Research2022，15，3： | 1 |
| 12 | CCGD-ESCC: A Comprehensive Database for Genetic Variants Associated with Esophageal Squamous Cell Carcinoma in Chinese Population显示文摘Esophageal squamous-cell carcinoma(ESCC) is one of the most lethal malignancies in the world and occurs at particularly higher frequency in China. While several genome-wide association studies(GWAS) of germline variants and whole-genome or whole-exome sequencing studies of somatic mutations in ESCC have been published, there is no comprehensive database publically available for this cancer. Here, we developed the Chinese Cancer Genomic Database-Esophageal Squamous Cell Carcinoma(CCGD-ESCC) database, which contains the associations of 69,593 single nucleotide polymorphisms(SNPs) with ESCC risk in 2022 cases and 2039 controls, survival time of 1006 ESCC patients(survival GWAS) and gene expression(expression quantitative trait loci,eQTL) in 94 ESCC patients. Moreover, this database also provides the associations between8833 somatic mutations and survival time in 675 ESCC patients. Our user-friendly database is a resource useful for biologists and oncologists not only in identifying the associations of genetic variants or somatic mutations with the development and progression of ESCC but also in studying the underlying mechanisms for tumorigenesis of the cancer. CCGD-ESCC is freely accessible at http://gffzzdf7d528e3a264cb9hok59k60fxxuw6von.ffgz.tsg.suse.edu.cn/ccgd/ESCCdb. | Linna Peng Sijin Cheng Yuan Lin Qionghua Cui Yingying Luo Jiahui Chu Mingming Shao Wenyi Fan Yamei Chen Ai Lin Yiyi Xi Yanxia Sun Lei Zhang Chao Zhang Wen Tan Ge Gao Chen Wu Dongxin Lin | 2018 | Genomics, Proteomics & Bioinformatics2018，16，4： | 1 |
| 13 | Laccase-functionalized magnetic framework composite enabled chlorophenols degradation,a potential remediation for fungicides residues in leather显示文摘Chlorophenols,used as the fungicides in leather,are strictly limited in leather products.In this work,a metal-organic framework material,zeolitic metal azolate framework-7(MAF-7),was first used to encapsulate laccase(Lac)to prepare MAF-7/Lac bio-composites with 98.5%immobilization yield.Afterward,Lac/MNP@MOM was formed by introducing the magnetic nanoparticles(MNPs)into the Lac@MOM.MAF-7 with better hydrophilicity and stronger pH buffering ability,exhibits good compatibility with laccase,which can reserve the activity of laccase after immobilization.Moreover,the porous structure of MAF-7 is favorable for the sufficient contact between laccase and substrates.Lac/MNP@MOM exhibited excellent activity when exposed to high temperature,extreme pH,and organic solvents,which also simplified complex recovery steps.Furthermore,the degradation rate of 2,4-dichlorophenol(2,4-DCP)could reach as high as 97%within 24 h by immobilized laccase,and after nine consecutive cycles of operation,enzyme activity could remain over 80%,which gives it the potential for practical applications. | Min Cao Jie Yu Xing Zhang Yamei Lin He Huang | 2022 | Journal of Leather Science and Engineering2022，4，1： | 0 |
| 14 | Highly active bifunctional catalyst: Constructing FeWO_(4)-WO_(3) heterostructure for water and hydrazine oxidation at large current density显示文摘Developing high performance anode catalysts for oxygen evolution reaction (OER) and hydrazine oxidation reaction (HzOR) at large current density is an efficient pathway to produce hydrogen. Herein, we synthesize a FeWO_(4)-WO_(3) heterostructure catalyst growing on nickel foam (FeWO_(4)-WO_(3)/NF) by a combination of hydrothermal and calcination method. It shows good catalytic activity with ultralow potentials for OER (ζ_(10) = 1.43 V, ζ_(1.000) = 1.56 V) and HzOR (ζ_(10) = −0.034 V, ζ_(1.000) = 0.164 V). Moreover, there is little performance degradation after being tested for _(10)0 h at 1,000 (OER) and _(10)0 (HzOR) mA·cm−2, indicating good stability. The superior performance could be attributed to the wolframite structure and heterostructure: The former provides a high electrical conductivity to ensure the electronic transfer capability, and the later induces interfacial electron redistribution to enhance the intrinsic activity and stability. The work offers a brand-new way to prepare good performance catalysts for OER and HzOR, especially at large current density. | Fang Shen Zhenglin Wang Yamei Wang Guangfu Qian Miaojing Pan Lin Luo Guoning Chen Hailang Wei Shibin Yin | 2021 | Nano Research2021，14，11： | 0 |
| 15 | Green Hydrothermal Synthesis and Applications of Sorbus pohuashanensis/Aronia melanocarpa Extracts Functionalized-Au/Ag/AuAg Nanoparticles显示文摘Nanoparticles(NPs)have already been widely used in catalysis,antibacterial and coating fields.Compared with the traditional toxic and harmful reducing reagents,green synthesis of NPs by using plant extracts is not only environmental-friendly and cost-effective but also conducive to the multi-level and efficient utilization of wild plant resources.In this study,the aqueous extracts from Sorbus pohuashanensis(SP)and Aronia melanocarpa(AM)fruits were used as the reducing and protective reagents for synthesizing Au/AgNPs,with the characteristics of originality operation and high repeatability.The SP/AM fruit extracts functionalized Au/AgNPs were characterized by UV-vis spectroscopy(UV-vis),transmission electron microscopy(TEM),energy dispersive spectroscopy(EDS),scanning electron microscope(SEM),X-ray diffraction(XRD)and Fourier transform infrared spectroscopy(FTIR).UV-vis spectrums showed the NPs peaks verified by the presence between 400–550 nm;TEM and SEM demonstrated NPs displayed approximately spherical structures;EDS confirmed the existence of Au/Ag elements;XRD measurements confirmed that the obtained NPs showed highly crystalline structures;FTIR demonstrated the fruits extracts were adsorbed on the surface of NPs.Primary experiments indicated that SP/AM fruit extracts functionalized-NPs could be used as the reagents for removing the organic dyes efficiently;Zone of inhibition tests(ZOI)explained that NPs have slow-release antibacterial activity. | Jin Huang Jixiang Sun Kai Shao Yamei Lin Zhiguo Liu Yujie Fu Liqiang Mu | 2023 | Journal of Renewable Materials2023，11，4： | 0 |
| 16 | Histone demethylase Kdm5c regulates osteogenesis and bone formation via PI3K/Akt/HIF1α and Wnt/β-catenin signaling pathways显示文摘Fractures have an extraordinarily negative impact on individuals'quality of life and functional status.Nonunion or disability of fracture is a major health issue with important clinical,social,and economic implications.1 Mesenchymal stem cells(MSCs)play an indispensable role in the initiation of the fracture repair process including the formation of a callus which is replaced by new bone.The use of MSCs in the treatment of fractures is very attractive as they can reduce the time of healing and occurrence of nonunion. | Ying Li Lingli Ding Yage Zhang Bingyu Zhou Gene Chi Wai Man Min Wang Jinglan Li Yamei Liu Weiping Lin Haibin Wang Sien Lin Liangliang Xu | 2024 | Genes & Diseases2024，11，1： | 0 |
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