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| 1 | ZmMs30 Encoding a Novel GDSL Lipase Is Essential for Male Fertility and Valuable for Hybrid Breeding in Maize显示文摘Genic male sterility (GMS) is very useful for hybrid vigor utilization and hybrid seed production. Although a large number of GMS genes have been identified in plants, little is known about the roles of GDSL lipase members in anther and pollen development. Here, we report a maize GMS gene, ZmMs30, which encodes a novel type of GDSL lipase with diverged catalytic residues. Enzyme kinetics and activity assays show that ZmMs30 has lipase activity and prefers to substrates with a short carbon chain. ZmMs30 is specifically expressed in maize anthers during stages 7-9. Loss of ZmMs30 function resulted in defective anther cuticle, irregular foot layer of pollen exine, and complete male sterility. Cytological and lipidomics analyses demonstrate that ZmMs30 is crucial for the aliphatic metabolic pathway required for pollen exine formation and anther cuticle development. Furthermore, we found that male sterility caused by loss of ZmMs30 function was stable in various inbred lines with different genetic background, and that it didn't show any negative effect on maize heterosis and production, suggesting that ZmMs30 is valuable for crossbreeding and hybrid seed production. We then developed a new multi-control sterility system using ZmMs30 and its mutant line, and demonstrated it is feasible for generating desirable GMS lines and valu. able for hybrid maize seed production. Taken together, our study sheds new light on the mechanisms of anther and pollen development, and provides a valuable male-sterility system for hybrid breeding maize. | Xueli An Zhenying Dong Youhui Tian Ke Xie Suowei Wu Taotao Zhu Danfeng Zhang Van Zhou Canfang Niu Biao Ma Quancan Hou Jianxi Bao Simiao Zhang Ziwen Li Yanbo Wang Tingwei Yan Xiaojing Sun Yuwen Zhang Jinping Li Xiangyuan Wan | 2019 | Molecular Plant2019，12，3： | 15 |
| 2 | Preparation,Characterization,and Catalytical Application of MgCoAl-Hydrotalcite-Like Compounds显示文摘MgCoAl-hydrotalcite-like 混合物(MgCoAl-HTLcs ) 用可变 pH 价值的 copre- cipitation 方法被综合。在 HTLcs 的准备的每个因素的效果系统地被讨论,它包括了热水的处理的 pH 值,到 Co2+ 的 Mg2+ 的鼹鼠比率,解决方案的集中,和温度和时间。而且, MgCoAl-HTLcs 的热 de- 作文被讨论。X 光检查衍射, thermogravimetric 分析,和英尺红外光谱学被执行描绘 MgCoAl-HTLcs 样品。结果看了那什么时候 pH = 7.6 8.5 或 5.5 6.2,原子比率(Mg2++Co2+) 是的热水的处理的从 1.00 ~ 2.00 的 /Al3+=2andMg2+/Co2+ ,温度和时间 110 ° C 和 6 h,有高 crystallinity 的唯一的 MgCoAl-HTLcs 能被获得。当锻烧温度直到 250 ° C 时, MgO 阶段被检测与 Al2O3 共存, MgAl2O4 高度在导出的混合氧化物上被散布。有甲醇和 benzaldehyde 的安息香甲基醚的合成反应被选择学习 MgCoAl-HTLcs 的催化活动。催化剂处于可行条件在安息香甲基醚的合成显示出高活动和高稳定性。 | Kai Yan Xianmei Xie Jinping Li Xiulan Wang Zhizhong Wang | 2007 | Journal of Natural Gas Chemistry2007，16，4： | 12 |
| 3 | Normal Structure and Function of Endothecium Chloroplasts Maintained by ZmMs33-Mediated Lipid Biosynthesis in Tapetal Cells Are Critical for Anther Development in Maize显示文摘Genic male sterility(GMS)is critical for heterosis utilization and hybrid seed production.Although GMS mutants and genes have been studied extensively in plants,it has remained unclear whether chloroplast-associated photosynthetic and metabolic activities are involved in the regulation of anther development.In this study,we characterized the function of ZmMs33/ZmGPAT6,which encodes a member of the glycerol-3-phosphate acyltransferase(GPAT)family that catalyzes the first step of the glycerolipid synthetic pathway.We found that normal structure and function of endothecium(En)chloroplasts maintained by ZmMs33-mediated lipid biosynthesis in tapetal cells are crucial for maize anther development.ZmMs33 is expressed mainly in the tapetum at early anther developmental stages and critical for cell proliferation and expansion at late stages.Chloroplasts in En cells of wild-type anthers function as starch storage sites before stage 10 but as photosynthetic factories since stage 10 to enable starch metabolism and carbohydrate supply.Loss of ZmMs33 function inhibits the biosynthesis of glycolipids and phospholipids,which are major components of En chloroplast membranes,and disrupts the development and function of En chloroplasts,resulting in the formation of abnormal En chloroplasts containing numerous starch granules.Further analyses reveal that starch synthesis during the day and starch degradation at night are greatly suppressed in the mutant anthers,leading to carbon starvation and low energy status,as evidenced by low trehalose-6-phosphate content and a reduced ATP/AMP ratio.The energy sensor and inducer of autophagy,SnRK1,was activated to induce early and excessive autophagy,premature PCD,and metabolic reprogramming in tapetal cells,finally arresting the elongation and development of mutant anthers.Taken together,our results not only show that ZmMs33 is required for normal structure and function of En chloroplasts but also reveal that starch metabolism and photosynthetic activities of En chloroplasts at different developmental stages are essential for normal anther development.These findings provide novel insights for understanding how lipid biosynthesis in the tapetum,the structure and function of En chloroplasts,and energy and substance metabolism are coordinated to maintain maize anther development. | Taotao Zhu Ziwen Li Xueli An Yan Long Xiaofeng Xue Ke Xie Biao Ma Danfeng Zhang Yijian Guan Canfang Niu Zhenying Dong Quancan Hou Lina Zhao Suowei Wu Jinping Li Weiwei Jin Xiangyuan Wan | 2020 | Molecular Plant2020，13，11： | 9 |
| 4 | Pharmacokinetics of H002, a novel S1PR_1 modulator, and its metabolites in rat blood using liquid chromatography–tandem mass spectrometry显示文摘A rapid and sensitive liquid chromatography–tandem mass spectrometry(LC–MS/MS) method was developed and validated for the simultaneous determination of H002 and its phosphorylated metabolite, H002-P and hydroxylated metabolite H002-M, in rat blood. H001, an analogue of H002, was used as the internal standard.Blood samples were prepared by simple protein precipitation. The analytes and internal standard were separated on a Zorbax SB-C18 column with a gradient mobile phase consisting of methanol and water containing 0.1% formic acid at a flow rate of 0.2 mL /min with an operating temperature of 20 1C. The detection was performed on a triple quadrupole tandem mass spectrometer with positive electrospray ionization in multiple-reaction monitoring mode.Linear detection responses were obtained from 0.2–100 ng/mL for H002 and H002-M, while 0.5–100 ng/mL for H002-P. The intra- and inter-day precision(RSD%) was within 11.76%, with the accuracy(RE%) ranging from –9.84% to 9.12%. The analytes were shown to be stable during sample storage, preparation and analytic procedures.The method was applied to determine the pharmacokinetics of H002 in rats, and a preliminary study showed that the pharmacokinetics of H002 correlated with its biological effect on peripheral blood lymphocytes. | Jiaqi Mi Manman Zhao Shu Yang Shuang Yang Jing Jin Xiaojian Wang Qiong Xiao Jinping Hu Yan Li | 2016 | Acta Pharmaceutica Sinica B2016，6，6： | 5 |
| 5 | Implantable nerve guidance conduits:Material combinations,multi-functional strategies and advanced engineering innovations显示文摘Nerve guidance conduits(NGCs)have attracted much attention due to their great necessity and applicability in clinical use for the peripheral nerve repair.Great efforts in recent years have been devoted to the development of high-performance NGCs using various materials and strategies.The present review provides a comprehensive overview of progress in the material innovation,structural design,advanced engineering technologies and multi functionalization of state-of-the-art nerve guidance conduits NGCs.Abundant advanced engineering technologies including extrusion-based system,laser-based system,and novel textile forming techniques in terms of weaving,knitting,braiding,and electrospinning techniques were also analyzed in detail.Findings arising from this review indicate that the structural mimetic NGCs combined with natural and synthetic materials using advanced manufacturing technologies can make full use of their complementary advantages,acquiring better biomechanical properties,chemical stability and biocompatibility.Finally,the existing challenges and future opportunities of NGCs were put forward aiming for further research and applications of NGCs. | Yixin Yan Ruotong Yao Jingyuan Zhao Kaili Chen Lirong Duan Tian Wang Shujun Zhang Jinping Guan Zhaozhu Zheng Xiaoqin Wang Zekun Liu Yi Li Gang Li | 2022 | Bioactive Materials2022，7，5： | 4 |
| 6 | Hydrodynamic Comparison of a Semi-submersible,TLP,and Spar:Numerical Study in the South China Sea Environment显示文摘The South China Sea contains tremendous oil and gas resources in deepwater areas. However, one of the keys for deepwater exploration, the investigation of deepwater floating platforms, is very inadequate. In this paper, the authors studied and compared the hydrodynamics and global motion behaviors of typical deepwater platforms in the South China Sea environment. The hydrodynamic models of three main types of floating platforms, e.g. the Semi-submersible, tension leg platform (TLP), and Truss Spar, which could potentially be utilized in the South China Sea, were established by using the 3-D potential theory. Additionally, some important considerations which significantly influence the hydrodynamics were given. The RAOs in frequency domains as well as global motions in time domains under time-varying wind, random waves, and current in 100-y, 10-y, and 1-y return period environment conditions were predicted, compared, and analyzed. The results indicate that the heave and especially the pitch motion of the TLP are favorable. The heave response of the Truss Spar is perfect and comparable with that of the TLP when the peak period of random waves is low. However, the pitch motion of Truss Spar is extraordinarily larger than that of Semi-submersible and TLP. | Binbin Li Kun Liu Gongwei Yan Jinping Ou | 2011 | Journal of Marine Science and Application2011，10，3： | 4 |
| 7 | Low sample volume origami-paper-based graphene-modified aptasensors for label-free electrochemical detection of cancer biomarker-EGFR显示文摘In this work,an electrochemical paper-based aptasensor was fabricated for label-free and ultrasensitive detection of epidermal growth factor receptor(EGFR)by employing anti-EGFR aptamers as the bio-recognition element.The device used the concept of paper-folding,or origami,to serve as a valve between sample introduction and detection,so reducing sampling volumes and improving operation convenience.Amino-functionalized graphene(NH 2-GO)/thionine(THI)/gold particle(AuNP)nanocomposites were used to modify the working electrode not only to generate the electrochemical signals,but also to provide an environment conducive to aptamer immobilization.Electrochemical characterization revealed that the formation of an insulating aptamer–antigen immunocomplex would hinder electron transfer from the sample medium to the working electrode,thus resulting in a lower signal.The experimental results showed that the proposed aptasensor exhibited a linear range from 0.05 to 200 ngmL^(−1)(R^(2)=0.989)and a detection limit of 5pgmL^(−1) for EGFR.The analytical reliability of the proposed paper-based aptasensor was further investigated by analyzing serum samples,showing good agreement with the gold-standard enzyme-linked immunosorbent assay. | Yang Wang Shuai Sun Jinping Luo Ying Xiong Tao Ming Juntao Liu Yuanyuan Ma Shi Yan Yue Yang Zhugen Yang Julien Reboud Huabing Yin Jonathan MCooper Xinxia Cai | 2020 | Microsystems & Nanoengineering2020，6，1： | 2 |
| 8 | Temporal and spatial changes of microbial community in an industrial effluent receiving area in Hangzhou Bay显示文摘Anthropogenic activities usually contaminate water environments, and have led to the eutrophication of many estuaries and shifts in microbial communities. In this study, the temporal and spatial changes of the microbial community in an industrial effluent receiving area in Hangzhou Bay were investigated by 454 pyrosequencing. The bacterial community showed higher richness and biodiversity than the archaeal community in all sediments. Proteobacteria dominated in the bacterial communities of all the samples; Marine_Group_Ⅰ and Methanomicrobia were the two dominant archaeal classes in the effluent receiving area. PCoA and AMOVA revealed strong seasonal but minor spatial changes in both bacterial and archaeal communities in the sediments. The seasonal changes of the bacterial community were less significant than those of the archaeal community, which mainly consisted of fluctuations in abundance of a large proportion of longstanding species rather than the appearance and disappearance of major archaeal species. Temperature was found to positively correlate with the dominant bacteria, Betaproteobacteria, and negatively correlate with the dominant archaea,Marine_Group_Ⅰ; and might be the primary driving force for the seasonal variation of the microbial community. | Yan Zhang Lujun Chen Renhua Sun Tianjiao Dai Jinping Tian Wei Zheng Donghui Wen | 2016 | Journal of Environmental Sciences2016，28，6： | 2 |
| 9 | Bayesian uncertainty analysis of SA turbulence model for supersonic jet interaction simulations显示文摘The Reynolds Averaged Navier-Stokes(RANS) models are still the workhorse in current engineering applications due to its high efficiency and robustness. However, the closure coefficients of RANS turbulence models are determined by model builders according to some simple fundamental flows, and the suggested values may not be applicable to complex flows, especially supersonic jet interaction flow. In this work, the Bayesian method is employed to recalibrate the closure coefficients of Spalart-Allmaras(SA) turbulence model to improve its performance in supersonic jet interaction problem and quantify the uncertainty of wall pressure and separation length. The embedded model error approach is applied to the Bayesian uncertainty analysis. Firstly, the total Sobol index is calculated by non-intrusive polynomial chaos method to represent the sensitivity of wall pressure and separation length to model parameters. Then, the pressure data and the separation length are respectively served as calibration data to get the posterior uncertainty of model parameters and Quantities of Interests(Qo Is). The results show that the relative error of the wall pressure predicted by the SA turbulence model can be reduced from 14.99% to 2.95% through effective Bayesian parameter estimation. Besides, the calibration effects of four likelihood functions are systematically evaluated. The posterior uncertainties of wall pressure and separation length estimated by different likelihood functions are significantly discrepant, and the Maximum a Posteriori(MAP) values of parameters inferred by all functions show better performance than the nominal values. Finally, the closure coefficients are also estimated at different jet total pressures. The similar posterior distributions of model parameters are obtained in different cases, and the MAP values of parameters calibrated in one case are also applicable to other cases. | Jinping LI Shusheng CHEN Fangjie CAI Sheng WANG Chao YAN | 2022 | Chinese Journal of Aeronautics2022，35，4： | 2 |
| 10 | The relationship of 3-PBA pyrethroids metabolite and male reproductive hormones among non-occupational exposure males显示文摘 | Yan Han Yankai Xia Jiayuan Han Jinping Zhou Shoulin Wang Pengfei Zhu Rencheng Zhao Nianzu Jin Ling Song Xinru Wang | 2008 | Chemosphere2008，，5： | 1 |
| 11 | Wireless sensing experiments for structural vibration monitoring of offshore platform显示文摘In order to validate the feasibility of applying wireless sensing technique to structural monitoring of offshore platform,the experiment of wireless sensor network on offshore platform is presented in this paper.First,wireless sensor network and its topology structure is put forward,and the design of sensor nodes,base station,communication protocol is discussed according to selfdeveloped wireless sensor network.Second,true offshore platform and its experimental model are introduced.Finally,wireless sensing experiment for offshore platform structure is completed and the analysis of the experimental result is given.The research shows that wireless sensor network applied to offshore platform can reflect the vibration of the structure;the sensor nodes are fixed and removed expediently,which saves the cost of signal line as well as installation time. | Yan YU Jinping OU | 2008 | Frontiers of Electrical and Electronic Engineering in China2008，3，3： | 1 |
| 12 | Ulinastatin as a neuroprotective and anti‐inflammatory agent in infant piglets model undergoing surgery on hypothermic low‐flow cardiopulmonary bypass显示文摘 | Xiaocou Wang Qinghua Xue Fuxia Yan Lihuan Li Jinping Liu Shoujun Li Shengshou Hu | 2013 | Paediatr Anaesth2013，，3： | 1 |
| 13 | wavelet transform 显示文摘 | Yan Guirong Duan Zhongdong Ou Jinping | 2010 | Mechanical Systems and Signal Processing2010，24，1： | 1 |
| 14 | Damage localization in ambient vibration by constructingproportional flexibility matrix 显示文摘 | Duan Zhongdong Yan Guirong Ou Jinping | 2005 | Journal of Sound andVibration2005，284，7： | 1 |
| 15 | A data loss recovery approach for wireless sensor networks using a compressive sampling technique显示文摘 | Bao Yuequan Li Hui Sun Xiaodan Yan Yu Ou Jinping | 2013 | Structural Health Monitoring-An International Journal2013，12，1： | 1 |
| 16 | Compressive sensingbased lost data recovery of fast-moving wireless sensing for structural health monitoring显示文摘 | Bao Yuequan Yan Yu Li Hui Mao Xingquan Jiao Wenfeng Zou Zilong Ou Jinping | 2014 | Structural Control and Health Monitoring2014，22，43： | 1 |
| 17 | Investigation of vortex-induced vibration of a suspension bridge with two separated steel box girders based on field measurements显示文摘 | Hui Li Shujin Laima Jinping Ou Xuefeng Zhao Wensong Zhou Yan Yu Na Li Zhiqiang Liu | 2011 | Engineering Structures2011，，6： | 1 |
| 18 | Surface roughness prediction of high speed milling based on back propagation ar- tificial neural net-work显示文摘 | HU Jinping LI Yan ZHANG Jingchong | 2010 | Advanced Materials Research2010，201，： | 1 |
| 19 | Molecular dynamics simulation of thermal conductivities of superlattice nanowires显示文摘 | Yang Juekuan Chen Yunfei Yan Jinping | 2003 | Science in China (Series E)2003，46，3： | 1 |
| 20 | Serum C-reactive protein and risk of lung cancer: a case–control study显示文摘 | Ming Xu Minglin Zhu Yinggan Du Bibo Yan Qian Wang Cong Wang Jinping Zhao | 2013 | Medical Oncology2013，，1： | 1 |
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