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| 1 | Pathological evidence for residual SARS-CoV-2 in pulmonary tissues of a ready-for-discharge patient显示文摘Dear Editor,SARS-CoV-2,a novel coronavirus and causing COVID-19,has given rise to a worldwide pandemic.1,2 So far,tens of thousands of COVID-19 patients have been clinically cured and discharged,but multiple COVID-19 cases showed SARS-CoV-2 positive again in discharged patients,3 which raises an attention for the discharged patients.Also,there is an urgent need to understand the pathogenesis of SARS-CoV-2 infection.Here,we conducted postmortem pathologic study in a ready-fordischarge COVID-19 patient who succumbed to sudden cardiovascular accident.Pathological examination revealed SARSCoV-2-viruses remaining in pneumocytes and virus-caused pathological changes in the lungs.Our study provided new insights into SARS-CoV-2 pathogenesis and might facilitate the improvement of clinical guideline for virus containment and disease management. | Xiao-Hong Yao Zhi-Cheng He Ting-Yuan Li Hua-Rong Zhang Yan Wang Huaming Mou Qiaonan Guo Shi-Cang Yu Yanqing Ding Xindong Liu Yi-Fang Ping Xiu-Wu Bian | 2020 | Cell Research2020，30，6： | 35 |
| 2 | The Genome Sequence Archive Family: Toward Explosive Data Growth and Diverse Data Types显示文摘The Genome Sequence Archive(GSA)is a data repository for archiving raw sequence data,which provides data storage and sharing services for worldwide scientific communities.Considering explosive data growth with diverse data types,here we present the GSA family by expanding into a set of resources for raw data archive with different purposes,namely,GSA(http://gffzz77e3413bc06540edsfnv5f9bq50nq6fn6.ffgz.tsg.suse.edu.cn/gsa/),GSA for Human(GSA-Human,http://gffzz77e3413bc06540edsfnv5f9bq50nq6fn6.ffgz.tsg.suse.edu.cn/gsa-human/),and Open Archive for Miscellaneous Data(OMIX,http://gffzz77e3413bc06540edsfnv5f9bq50nq6fn6.ffgz.tsg.suse.edu.cn/omix/).Compared with the 2017 version,GSA has been significantly updated in data model,online functionalities,and web interfaces.GSA-Human,as a new partner of GSA,is a data repository specialized in human genetics-related data with controlled access and security.OMIX,as a critical complement to the two resources mentioned above,is an open archive for miscellaneous data.Together,all these resources form a family of resources dedicated to archiving explosive data with diverse types,accepting data submissions from all over the world,and providing free open access to all publicly available data in support of worldwide research activities. | Tingting Chen Xu Chen Sisi Zhang Junwei Zhu Bixia Tang Anke Wang Lili Dong Zhewen Zhang Caixia Yu Yanling Sun Lianjiang Chi Huanxin Chen Shuang Zhai Yubin Sun Li Lan Xin Zhang Jingfa Xiao Yiming Bao Yanqing Wang Zhang Zhang Wenming Zhao | 2021 | Genomics, Proteomics & Bioinformatics2021，19，4： | 38 |
| 3 | 3RISPR-P 2.0: An Improved CRISPR-Cas9 Too For Genome Editing in Plants显示文摘 | Hao Liu Yuduan Ding Yanqing Zhou Wenqi Jin Kabin Xie Ling-Ling Chen | 2017 | Molecular Plant2017，10，3： | 32 |
| 4 | Hypoxia inducible factor(HIF) in the tumor microenvironment:friend or foe?显示文摘Hypoxia acts as an important regulator of physiological and pathological processes. Hypoxia inducible factors(HIFs) are the central players involved in the cellular adaptation to hypoxia and are regulated by oxygen sensing EGLN prolyl hydroxylases.Hypoxia affects many aspects of cellular growth through both redox effects and through the stabilization of HIFs. The HIF isoforms likely have differential effects on tumor growth via alteration of metabolism, growth, and self-renewal and are likely highly context-dependent. In some tumors such as renal cell carcinoma, the EGLN/HIF axis appears to drive tumorigenesis,while in many others HIF1 and HIF2 may actually have a tumor suppressive role. An emerging role of HIF biology is its effects on the tumor microenvironment. The EGLN/HIF axis plays a key role in regulating the function of the various components of the tumor microenvironment, which include cancer-associated fibroblasts, endothelial cells, immune cells, and the extracellular matrix(ECM). Here, we discuss hypoxia and the diverse roles of HIFs in the setting of tumorigenesis and the maintenance of the tumor microenvironment as well as possible future directions of the field. | Yanqing Huang Daniel Lin Cullen M.Taniguchi | 2017 | Science China(Life Sciences)2017，60，10： | 26 |
| 5 | p53 modifications:exquisite decorations ofthe powerful guardian显示文摘The last 40 years have witnessed how p53 rose from a viral binding protein to a central factor in both stress responses and tumor suppression.The exquisite regulation of p53 functions is of vital importance for cell fate decisions.Among the multiple layers of mechanisms controlling p53 function,posttranslational modifications (PTMs) represent an efficient and precise way.Major p53 PTMs include phosphorylation,ubiquitination,acetylation,and methylation.Meanwhile,other PTMs like sumoylation,neddylation,O-GlcNAcylation,adenosine diphosphate (ADP)-ribosylation,hydroxylation,and p-hydroxybutyrylation are also shown to play various roles in p53 regulation.By independent action or interaction,PTMs affect p53 stability,conformation,localization,and binding partners.Deregulation of the PTM-related pathway is among the major causes of p53-associated developmental disorders or diseases,especially in cancers.This review focuses on the roles of different p53 modification types and shows how these modifications are orchestrated to produce various outcomes by modulating p53 activities or targeted to treat different diseases caused by p53 dysregulation. | Yanqing Liu Omid Tavana Wei Gu | 2019 | Journal of Molecular Cell Biology2019，11，7： | 24 |
| 6 | Standardized training for resident doctors in China显示文摘Medical graduates are not fully trained physicians when they leave medical school.Currently,standardized training for resident doctors in most hospitals in China is carried out according to the Ministry of Health guidelines.However,these guidelines provide only outline guidance,and specific resident training is managed independently by and varies with individual institutions.Therefore,it is important to study training demand to learn about the content and emphasis of training. | Tonglin Sun Yanqing Wang | 2014 | The Journal of Biomedical Research2014，28，3： | 23 |
| 7 | Optimal time to use low molecular weight heparin on prethrombotic state of rat chronic obstructive pulmonary disease model显示文摘 | Wang Yanqing Sun Nina Cheng Zhaozhong Tong Li | 2014 | Chinese Medical Journal2014，，3： | 19 |
| 8 | Update in version 2021 of CSCO guidelines for colorectal cancer from version 2020显示文摘Colorectal cancer(CRC) is one of the major cancers threatening life and health of Chinese residents. According to the latest data released by the National Cancer Center in2015(1), there were 387,000 new cases and 187,100 deaths of CRC in China, accounting for 9.87% and 8.01% of all malignancies. The first version of the Chinese Society of Clinical Oncology(CSCO) guidelines was released in April2017, and has been updated annually based on the latest clinical research data and the availability of new drugs(2-4). Here, we present the main updates of the 2021 version compared to 2020 version. | Caixia Dong Yuwei Ding Shanshan Weng Guichao Li Yanqing Huang Hanguang Hu Zhen Zhang Suzhan Zhang Ying Yuan | 2021 | Chinese Journal of Cancer Research2021，33，3： | 19 |
| 9 | An overview of the use of plastic-film mulching in China to increase crop yield and water-use efficiency显示文摘Since 1990,crop yields have stagnated in many parts of the world,posing a significant challenge to global food security[1].As such;innovative solutions are needed to increase the resource-use efficiency of cropping systems to produce more grains per unit area. | Dongbao Sun Haigang Li Enli Wang Wenqing He Weiping Hao Changrong Yan Yuzhong Li Xurong Mei Yanqing Zhang Zhanxiang Sun Zhikuan Jia Huaiping Zhou Tinglu Fan Xucheng Zhang Qin Liu Fengju Wang Chaochun Zhang Jianbo Shen Qingsuo Wang Fusuo Zhang | 2020 | National Science Review2020，7，10： | 19 |
| 10 | Celastrus orbiculatus extract induces mitochondrial-mediated apoptosis in human hepatocellular carcinoma cells显示文摘OBJECTIVE:To investigate the apoptotic effects and underlying molecular mechanisms of Celastrus orbiculatus(C.orbiculatus) extract in human hepatocellular carcinoma cells.METHODS:Human hepatocellular carcinoma cells(HCCLM6) were treated with C.orbiculatus extract(COE) at different nontoxic concentrations(10,20,40,80,and 160 μg/mL).The effect of COE on HC-CLM6 viability was examined using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide(MTT) assays.Cellular apoptosis following COE treatment was assessed by flow cytometry and western blot analysis.RESULTS:COE significantly inhibited cell viability and induced apoptosis of HCCLM6 cells in a dose-dependent manner.Apoptosis was accompanied by increased Bax expression and decreased Bcl-2 expression.In addition,COE treatment led to the release of cytochrome c,activation of caspase-3,and cleavage of poly(ADP-ribose) polymerase(PARP).Furthermore,activation of extracellular signal-regulated kinase(ERK),p38 kinase,and c-Jun N-terminal kinase(JNK) phosphorylation,and down-regulation of Akt phosphorylation was observed.CONCLUSION:COE induces mitochondrial-mediated,caspase-dependent apoptosis in HCCLM6 cells,which might be attributed to the activation of mitogen-activated protein kinase(MAPK) and inhibition of Akt signaling pathways.These data suggest that COE may be a potential treatment for human hepatocellular carcinoma. | Hua Zhang Yayun Qian Yanqing Liu Guoqing Li Pingfang Cui Yaodong Zhu Hui Ma Xue Ji Shiyu Guo Hisamits Tadashi | 2012 | Journal of Traditional Chinese Medicine2012，32，4： | 18 |
| 11 | 秸秆处理技术的最新进展:挑战与解决方案显示文摘秸秆作为一种农业副产品已被认为是一种潜在生物质资源。然而,露天焚烧秸秆仍是世界许多地区的主要手段,这造成资源浪费和空气污染。近年来已经开发了许多用于从秸秆中回收能源和资源的技术,由于其经济可行性和生态友好性质,该生物学方法引起了越来越多的研究兴趣。然而,在生物加工过程之前对秸秆进行预处理是必不可少的,并且在很大程度上决定了过程的可行性、经济可行性和环境可持续性。因此,本综述试图对当前的秸秆预处理技术和管理实践进行重要而全面的分析。主要提出了结合微生物降解和酶促水解的综合生物过程,并阐述了其与未来大规模秸秆处理相关的潜在利益、局限性和挑战,并展望了前景和方向。 | Yingqun Ma Yanqing Shen Yu Liu | 2020 | 中国农机化学报2020，41，11： | 18 |
| 12 | NOD2 3020insC frameshift mutation is not associated with inflammatory bowel disease in Chinese patients of Hart nationality显示文摘AIM: An insertion mutation at nucleotide 3020 (3020insC) in the Caspase recruitment domain gene (CARD15), originally reported as NOD2, is strongly associated with Crohn's disease. The C-insertion mutation at nucleotide 3020 (3020inC) in the leucine-rich repeat (LRR) region results in a frameshift in the 10^th LRR followed by a premature stop codon. This truncation mutation is responsible for the inability to activate nuclear factor (NF)-κB in response to bacterial lipopolysaccharide (LPS). The present study aimed to genotype NOD2/CARD15 gene 3020insC frameshift mutation in Chinese patients with inflammatory bowel disease. METHODS: We genotyped an insertion polymorphism affecting the leucine-rich region of the protein product by the allele specific PCR in 74 unrelated patients with ulcerative colitis of Han nationality in Hubei Province of China, 15 patients with Crohn's disease and 172 healthy individuals. RESULTS: No significant differences were found in the genotype and allele frequendes of the C-insertion mutation of NOD2 gene among patients with Crohn's disease and ulcerative colitis and healthy controls. CONCLUSION: NOD2 gene 3020insC frameshift mutation is not a major contributor to the susceptibility to both Crohn's disease and ulcerative colitis in Chinese Han patients. | Qiu-ShaGuo BingXia YiJiang YanQü JingLi | 2004 | World Journal of Gastroenterology2004，10，7： | 16 |
| 13 | Effect and mechanism of Irbesartan on occurrence of ventricular arrhythmias in rats with myocardial ischemia through connexin43(cx43)显示文摘Objective: To explore the effect and mechanism of angiotensin Ⅱ receptor blockers-Irbesartan on occurrence of ventricular arrhythmias in rats with myocardial ischemia. Methods: Rats with embryonic cardiomyocytes-H9c2 were randomly divided into control group, ischemia group, Irbesartan group and Irbesartan+ischemia group. The cell viability of rats in each group was tested using MTT. Real-time PCR was employed to detect the expression of connexin43(Cx43) mR NA and western blot to detect the expression of Cx43 and phosphorylated Cx43. SD rats were randomly divided into the sham-operation group(SO), myocardial infarction group(MI), Irbesartan group and MI+ Irbesartan group, with 10 rats in each group. HE staining was employed to observe the change in the pathomorphology of left ventricular tissue and TUNEL method to analyze the cell apoptosis in the tissue. The immunofluorescence was adopted to observe the expression and distribution of Cx43 in the left ventricular myocardium and study the change in the expression of Cx43 in the cardiac muscular tissue at mR NA and protein level. Results: The intervention of Irbesartan in the condition of ischemia indicated the significant decrease in the number of necrotic cells. The expression of Cx43 was significantly decreased under the culture of ischemia(P<0.05), but in the presence of Irbesartan, the expression of Cx43 was increased compared with the ischemia group(P<0.01). The results of WB assay showed the similar trend of change at mRNA level. There was the significant difference in the score of ventricular arerythmia between MI group and SO group(P<0.01). The incidence of ventricular tachycardia or ventricular fibrillation was significantly increased compared with the one in SO group(P<0.05). There was the significant difference in the overall score between MI+Irbesartan group and MI group(P<0.05). The expression of Cx43 in the cardiac muscular tissue in MI group was significantly decreased(P<0.01 vs SO group). But the expression of Cx43 was increased after the treatment with Irbesartan. Conclusions: Irbesartan can inhibit the injury of H9c2 cardiomyocytes and the decreased expression of Cx43 that are induced by the ischemic myocardial infarction. Irbesartan can also improve the reconstruction of Cx43 in rats with ischemic myocardium to inhibit the myocardial infarction-induced arrhythmias. | Tao Wu Dan Wu Qinghua Wu Bing Zou Xiao Huang Xiaoshu Cheng Yanqing Wu Kui Hong Ping Li | 2016 | Asian Pacific Journal of Tropical Medicine2016，9，10： | 15 |
| 14 | Catalytic fast pyrolysis of Kraft lignin with HZSM-5 zeolite for producing aromatic hydrocarbons显示文摘 | Xiangyu LI Lu SU Yujue WANG Yanqing YU Chengwen WANG Xiaoliang LI Zhihua WANG | 2012 | Frontiers of Environmental Science & Engineering2012，6，3： | 14 |
| 15 | miR-181b functions as an oncomiR in colorectal cancer by targeting PDCDZ显示文摘 | Yanqing Liu Uzair-ur-Rehman Yu Guo Hongwei Liang Rongjie Cheng Fei Yang Yeting Hong Chihao Zhao Minghui Liu Mengchao Yu Xinyan Zhou Kai Yin Jiangning Chen Junfeng Zhang Chen-Yu Zhang Feng Zhi Xi Chen | 2016 | Protein & Cell2016，7，10： | 14 |
| 16 | PepperHub, an Informatics Hub for the Chili Pepper Research Community显示文摘 | Feng Liu Huiyang Vu Yingtian Deng Jingyuan Zheng Minglei Liu Lijun Ou Bozhi Yang Xiongze Dai Yanqing Ma Shengyu Feng Shuang He Xuefeng Li Zhuqing Zhang Wenchao Chen Shudong Zhou Rong Chen Minmin Liu Sha Yang Ruimin Wei Huadong Li Feng Li Bo Ouyang Xuexiao Zou | 2017 | Molecular Plant2017，10，8： | 13 |
| 17 | GSA:Genome Sequence Archive显示文摘With the rapid development of sequencing technologies towards higher throughput and lower cost, sequence data are generated at an unprecedentedly explosive rate. To provide an efficient and easy-to-use platform for managing huge sequence data, here we present Genome Sequence Archive(GSA; http://gffzzdbc7b6aaae734bddhfnv5f9bq50nq6fn6.ffgz.tsg.suse.edu.cn/gsa or http://gffzz4eaf941a184140e3hfnv5f9bq50nq6fn6.ffgz.tsg.suse.edu.cn), a data repository for archiving raw sequence data. In compliance with data standards and structures of the International Nucleotide Sequence Database Collaboration(INSDC), GSA adopts four data objects(Bio Project, Bio Sample,Experiment, and Run) for data organization, accepts raw sequence reads produced by a variety of sequencing platforms, stores both sequence reads and metadata submitted from all over the world,and makes all these data publicly available to worldwide scientific communities. In the era of big data, GSA is not only an important complement to existing INSDC members by alleviating the increasing burdens of handling sequence data deluge, but also takes the significant responsibility for global big data archive and provides free unrestricted access to all publicly available data in support of research activities throughout the world. | Yanqing Wang Fuhai Song Junwei Zhu Sisi Zhang Yadong Yang Tingting Chen Bixia Tang Lili Dong Nan Ding Qian Zhang Zhouxian Bai Xunong Dong Huanxin Chen Mingyuan Sun Shuang Zhai Yubin Sun Lei Yu Li Lan Jingfa Xiao Xiangdong Fang Hongxing Lei Zhang Zhang Wenming Zhao | 2017 | Genomics, Proteomics & Bioinformatics2017，15，1： | 13 |
| 18 | Gray bootstrap method for estimating frequency-varying random vibration signals with small samples显示文摘During environment testing, the estimation of random vibration signals(RVS) is an important technique for the airborne platform safety and reliability. However, the available methods including extreme value envelope method(EVEM), statistical tolerances method(STM) and improved statistical tolerance method(ISTM) require large samples and typical probability distribution. Moreover, the frequency-varying characteristic of RVS is usually not taken into account. Gray bootstrap method(GBM) is proposed to solve the problem of estimating frequency-varying RVS with small samples. Firstly, the estimated indexes are obtained including the estimated interval, the estimated uncertainty, the estimated value, the estimated error and estimated reliability. In addition, GBM is applied to estimating the single flight testing of certain aircraft. At last, in order to evaluate the estimated performance, GBM is compared with bootstrap method(BM) and gray method(GM) in testing analysis. The result shows that GBM has superiority for estimating dynamic signals with small samples and estimated reliability is proved to be 100% at the given confidence level. | Wang Yanqing Wang Zhongyu Sun Jianyong Zhang Jianjun Zissimos Mourelato | 2014 | Chinese Journal of Aeronautics2014，27，2： | 12 |
| 19 | Effect of allogeneic umbilical cord mesenchymal stem cell transplantation in a rat model of hepatic cirrhosis显示文摘OBJECTIVE: To determine the effects of human umbilical cord mesenchymal stem cell(UCMSC) transplantation, alone or in combination with tanshinone IIA(Tan IIA) on hepatic cirrhosis in rats.METHODS: A rat model of cirrhosis was established. Rats were divided into control, UCMSC, and UCSMC plus Tan IIA groups. Rats in the UCMSC group were injected via the tail vein with 0.2 m L Di I-labeled UCMSC suspension. Intraperitoneal Tan IIA injections(20 mg/kg) were started on the day ofUCMSC transplantation in the UCMSC plus Tan IIA group, and continued for 7 consecutive days thereafter. Rats were sacrificed 1 day, 3 days, 1 month,and 3 months after transplantation and the numbers of Di I-labeled UCMSCs colonizing the liver were determined. Albumin(ALB) and alanine aminotransferase(ALT) levels were measured in venous blood, and m RNA and protein expression levels of human ALB and cytokeratin(CK)-18 in liver tissues were determined by reverse transcription-polymerase chain reaction and western blotting, respectively.RESULTS: Serum ALT levels were significantly lower and serum ALB levels significantly higher in rats in the UCMSC group compared with the control group(P < 0.05). Hepatic CK-18 and ALB m RNA and protein expression levels increased after transplantation, and were significantly higher in the UCMSC plus Tan IIA group compared with the UCMSC group(P < 0.05).CONCLUSION: Human UCMSCs transplanted into rats with liver cirrhosis can grow and differentiate into hepatocyte-like cells resulting in improved liver function in vivo. Tan IIA further influenced transplantation outcomes. | Yang Lihua Wang Yun Wang Xiaohua Liu Yanqing | 2015 | Journal of Traditional Chinese Medicine2015，35，1： | 12 |
| 20 | Agricultural information dissemination using ICTs:A review and analysis of information dissemination models in China显示文摘Over the last three decades,China’s agriculture sector has been transformed from the traditional to modern practice through the effective deployment of Information and Communication Technologies(ICTs).Information processing and dissemination have played a critical role in this transformation process.Many studies in relation to agriculture information services have been conducted in China,but few of them have attempted to provide a comprehensive review and analysis of different information dissemination models and their applications.This paper aims to review and identify the ICT based information dissemination models in China and to share the knowledge and experience in applying emerging ICTs in disseminating agriculture information to farmers and farm communities to improve productivity and economic,social and environmental sustainability.The paper reviews and analyzes the development stages of China’s agricultural information dissemination systems and different mechanisms for agricultural information service development and operations.Seven ICT-based information dissemination models are identified and discussed.Success cases are presented.The findings provide a useful direction for researchers and practitioners in developing future ICT based information dissemination systems.It is hoped that this paper will also help other developing countries to learn from China’s experience and best practice in their endeavor of applying emerging ICTs in agriculture information dissemination and knowledge transfer. | Yun Zhang Lei Wang Yanqing Duan | 2016 | Information Processing in Agriculture2016，3，1： | 12 |
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