|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | CRISPR/Cas9-mediated gene editing in human tripronuclear zygotes显示文摘染色体编辑工具例如定期聚类短 palindromic 重复(CRISPR ) 联系了的 interspaced 系统(Cas ) 广泛地被用来包括动物接合子和人的房间在模型系统修改基因,并且为基本研究和临床的应用保持巨大的诺言。迄今为止,严肃的知识差距在人的早胚胎,并且在效率和在人的培植前胚胎使用象 CRISPR/Cas9 那样的技术的潜在的离开目标效果留在我们 DNA 修理机制的理解。在这份报告,我们使用了推进的接合子调查 CRISPR/Cas9-mediated 基因在人的房间编辑的 tripronuclear (3PN ) 。我们发现 CRISPR/Cas9 能有效地劈开内长的 -globin 基因(HBB ) 。然而,相应再结合的效率指导了 HBB 的修理(HDR ) 是低的,编辑胚胎是马赛克。离开目标劈开在由 T7E1 试金并且 whole-exome 定序揭示了的这些 3PN 接合子也是明显的。而且,内长的 delta-globin 基因(HBD ) 对 HBB 相应,与外长的施主 oligos 竞争了充当修理模板,导致倔强的变化。我们的数据也显示在这些胚胎的 HBB 地点的修理通过非转线路 HDR 小径优先地发生了。一起拿,我们的工作加亮紧迫的需要进一步改进 CRISPR/Cas9 平台的忠实和特性,为编辑的 CRSIPR/Cas9-mediated 的任何临床的应用程序的一个前提。 | Puping Liang Yanwen Xu Xiya Zhang Chenhui Ding Rui Huang Zhen Zhang Jie Lv Xiaowei Xie Yuxi Chen Yujing Li Ying Sun Yaofu Bai Zhou Songyang Wenbin Ma Canquan Zhou Junjiu Huang | 2015 | Protein & Cell2015，6，5： | 149 |
| 2 | Human mesenchymal stromal cells enhance the immunomodulatory function of CD8+CD28- regulatory T cells显示文摘 | Qiuli Liu Haiqing Zheng Xiaoyong Chen Yanwen Peng Weijun Huang Xiaobo Li Gang Li Wenjie Xia Qiquan Sun an Andy Peng Xiang | 2015 | Cellular & Molecular Immunology2015，12，6： | 12 |
| 3 | Genetic and biological characteristics of the globally circulating H5N8 avian influenza viruses and the protective efficacy offered by the poultry vaccine currently used in China显示文摘The H5N8 avian influenza viruses have been widely circulating in wild birds and are responsible for the loss of over 33 million domestic poultry in Europe, Russia, Middle East, and Asia since January 2020. To monitor the invasion and spread of the H5N8 virus in China, we performed active surveillance by analyzing 317 wild bird samples and swab samples collected from 41,172 poultry all over the country. We isolated 22 H5N8 viruses from wild birds and 14 H5N8 viruses from waterfowls. Genetic analysis indicated that the 36 viruses formed two different genotypes: one genotype viruses were widely detected from different wild birds and domestic waterfowls;the other genotype was isolated from a whopper swan. We further revealed the origin and spatiotemporal spread of these two distinct H5N8 virus genotypes in 2020 and 2021. Animal studies indicated that the H5N8 isolates are highly pathogenic to chickens, mildly pathogenic in ducks, but have distinct pathotypes in mice. Moreover, we found that vaccinated poultry in China could be completely protected against H5N8 virus challenge. Given that the H5N8 viruses are likely to continue to spread in wild birds, vaccination of poultry is highly recommended in high-risk countries to prevent H5N8 avian influenza. | Pengfei Cui Xianying Zeng Xuyong Li Yanbing Li Jianzhong Shi Conghui Zhao Zhiyuan Qu Yanwen Wang Jing Guo Wenli Gu Qi Ma Yuancheng Zhang Weipeng Lin Minghui Li Jingman Tian Dongxue Wang Xin Xing Yanjing Liu Shuxin Pan Yaping Zhang Hongmei Bao Liling Liu Guobin Tian Chengjun Li Guohua Deng Hualan Chen | 2022 | Science China(Life Sciences)2022，65，4： | 12 |
| 4 | Performance analysis of air-water dual source heat pump water heater with heat recovery显示文摘A new air-water dual source heat pump water heater with heat recovery is proposed.The heat pump system can heat water by using a single air source,a single water source,or air-water dual sources.The water is first pre-heated by waste hot water,then heated by the heat pump.Waste heat is recovered by first preheating the cold water and as water source of the heat pump.According to the correlated formulas of the coefficient of performance of air-source heat pump and water-source heat pump,and the gain coefficient of heat recovery-preheater,the formulas for the coefficient of performance of heat pump in six operating modes are obtained by using the dimensionless correspondence analysis method.The system characteristics of heat absorption and release associated with the heat recovery-preheater are analyzed at different working conditions.The developed approaches can provide reference for the optimization of the operating modes and parameters.The results of analysis and experiments show that the coefficient of performance of the device can reach 4-5.5 in winter,twice as much as air source heat pump water heater.The utilization of waste heat in the proposed system is higher than that in the system which only uses waste water to preheating or as heat source.Thus,the effect of energy saving of the new system is obvious.On the other hand,the dimensionless correspondence analysis method is introduced to performance analysis of the heat pump,which also has theoretical significance and practical value. | CHEN ZeShao TAO WenQuan ZHU YanWen HU Peng | 2012 | Science China(Technological Sciences)2012，55，8： | 9 |
| 5 | Effect of melt superheating treatment on the cast structure of K465 nickel-based superalloy显示文摘The cast structure of a K465 nickel-based superalloy with different temperatures of melt superheating treatment was studied. It is shown that melt superheating treatment plays a significant role in the grain size and carbide morphology. With increasing melt superheating temperature (below 2023 K),the grain size increases obviously and the carbide morphology is changed from a blocky to a script-like shape. However,when the melt superheating temperature is between 2023 K and 2123 K,the grain size decreases gradually,and the morphology of the MC carbide changes back to the blocky appearance. The content of W is also influenced by melt superheating treatment. | Pei, Zhongye Zhao, Minghan Tian, Yanwen Li, Juntao Chen, Xingfu Wu, Jiantao | 2009 | Rare Metals2009，28，2： | 5 |
| 6 | Wafer-scale arrayed p-n junctions based on few-layer epitaxial GaTe显示文摘二维(2D ) 材料吸引了实质的注意在电子并且有灵活、透明、高度悦耳的优异优点的 optoelectronic 应用。当时,无差距的 graphene 展出极端宽带、快的 photoresponse 半导体的瞬间 2 和门高展出的 2D 敏感和悦耳的 responsivity 到可见的光。然而,设备收益和重覆性打电话让进一步的改进完成大规模一致性。这里,我们与 28.4 厘米 2/(V0 的高洞活动性报导晶片规模门的 layer-by-layer | Xiang Yuan Lei Tang Peng Wang Zhigang Chen Yichao Zou Xiaofeng Su Cheng Zhang Yanwen Liu Weiyi Wang Cong Liu Fansheng Chen Jin Zou Peng Zhou Weida Hu Faxian Xiu | 2015 | Nano Research2015，8，10： | 4 |
| 7 | Nitrogen-doped carbon nanotube/polyaniline composite:Synthesis, characterization, and its application to the detection of dopamine显示文摘Nitrogen-doped carbon nanotubes (N-CNTs)/polyaniline (PANI) composites are developed as an electrode material for biosensors. The morphology, composition, and optical properties of the resulting products were characterized by transmission electron microscopy (TEM), thermogravimetric analysis (TGA), Fourier transform infrared spectroscopy (FT-IR), and ultraviolet-visible absorption spectra (UV-vis). Furthermore, N-CNTs/PANI composite was immobilized on the surface of a glassy carbon electrode (GCE) and applied to construct a sensor. The obtained N-CNTs/PANI-modified GCE showed one pair of redox peaks and high catalytic activity for the oxidation of dopamine (DA) in a neutral environment. Differential pulse voltam-mograms results illustrate that the fabricated DA biosensor has high anti-interference ability towards ascorbic acid (AA). In addition, the fabricated biosensor showed superior performances with two wide linear ranges from 1 to 80 μM and from 1.5 to 3.5 mM and a low detection limit of 0.01 μM. | FENG XiaoMiao LI RuiMei MA YanWen CHEN RunFeng MEI QunBo FAN QuLi HUANG Wei | 2011 | Science China Chemistry2011，54，10： | 3 |
| 8 | Optical superoscillatory waves without side lobes along a symmetric cut显示文摘Optical superoscillation refers to an intriguing phenomenon of a wave packet that can oscillate locally faster than its highest Fourier component,which potentially produces an extremely localized wave in the far field.It provides an alternative way to overcome the diffraction limit,hence improving the resolution of an optical microscopy system.However,the optical superoscillatory waves are inevitably accompanied by strong side lobes,which limits their fields of view and,hence,potential applications.Here,we report both experimentally and theoretically a new superoscillatory wave form,which not only produces significant feature size down to deep subwavelength,but also completely eliminates side lobes in a particular dimension.We demonstrate a new mechanism for achieving such a wave form based on a pair of moonlike sharp-edge apertures.The resultant superoscillatory wave exhibits Bessel-like forms,hence allowing long-distance propagation of subwavelength structures.The result facilitates the study of optical superoscillation and on a fundamental level eliminates the compromise between the superoscillatory feature size and the field of view. | Yanwen Hu Shiwang Wang Junhui Jia Shenhe Fu Hao Yin Zhen Li Zhenqiang Chen | 2021 | Advanced Photonics2021，3，4： | 3 |
| 9 | 原纤维蛋白-1 mRNA和蛋白在子宫肌瘤组织中的表达显示文摘目的:探讨原纤维蛋白-1(fibrillin-1,FBN-1)mRNA和蛋白在子宫肌瘤组织中的表达及其与月经周期的关系。方法:50份子宫肌瘤及同源正常子宫平滑肌组织标本在术中被采集。根据月经周期及子宫内膜组织病理学检查分为增生期、分泌期及萎缩期。采用实时荧光定量PCR、Western blot分析及免疫组织化学方法检测FBN-1 mRNA和蛋白的表达。结果:免疫组织化学染色显示FBN-1在子宫肌瘤及正常子宫平滑肌组织的细胞外基质中均有较丰富的表达。FBN-1 mRNA和蛋白在增生期和分泌期中的子宫肌瘤组织中的表达均明显高于同源正常子宫平滑肌组织(P<0.01,P<0.05;P<0.01,P<0.05),而在绝经期中的子宫平滑肌瘤及正常子宫平滑肌组织的表达无明显差异。结论:FBN-1可能与子宫肌瘤的发生机制有关,并可能受激素调节的影响。 | 赵扬玉 乔杰 Yanwen Bertha Chen | 2007 | 实用妇产科杂志2007，23，1： | 1 |
| 10 | The singularity principle and property of Stewart parallel manipulator显示文摘 | Huang Zhen Chen Longhui Li Yanwen | 2003 | Journal of Robotic Systems2003，20，4： | 1 |
| 11 | Object tracking using learned feature manifolds显示文摘 | GUO Yanwen CHEN Ye TANG Feng | 2014 | Computer Vision and Image Understanding2014，118，： | 1 |
| 12 | Fabrication and characteristics of nano LiFePO 4 /C composites with high capacity and high rate using nano Fe 2 O 3 as raw materials显示文摘 | BaoHua Rong YanWen Lu XueWen Liu QingLin Chen Kun Tang HuaZhen Yang XingYun Wu Fei Shen YanBin Chen YueFeng Tang YanFeng Chen | 2014 | Nano Energy2014，，： | 1 |
| 13 | Critical role of phosphoinositide 3-kinase cascade in adipogenesis of human mesenchymal stem cells显示文摘 | Weihua Yu Zhenguang Chen Jinli Zhang Lirong Zhang Hui Ke Lihua Huang Yanwen Peng Xiuming Zhang Shunong Li Bruce T. Lahn Andy Peng Xiang | 2008 | Molecular and Cellular Biochemistry (-)2008，，1： | 1 |
| 14 | The singularity principle and property of stewart parallel manipulator显示文摘 | HUANG Zhen CHEN Longhui LI Yanwen | 2003 | Journal of Robotic Systems2003，20，4： | 1 |
| 15 | Emission and Surface Characteristic of Ternary Alloy Ir/Re/W Coated Impregnated Tungsten Cathodes显示文摘 | Zhang Honglai Liu Yanwen Chen ming | 2005 | Applied Surface Science2005，251，： | 1 |
| 16 | The singularity principle and property of Stewart manipulator显示文摘 | HUANG Zhen CHEN Longhui LI Yanwen | 2003 | Journal of Robotic Systems2003，20，4： | 1 |
| 17 | Design of Ningdu Talent Apartment Design in Jiangxi显示文摘Taking the design of Ningdu Talent Apartment for example,active eco-design at the origin of design—design of building scheme was elaborated,including overall layout,planar design,eco-interface design,building environment and outer space design,sun-shading design.Implementation of ecological and energysaving concepts in building scheme was reviewed. | LENG Haoran CHEN Xiaogang YANG Yanwen | 2013 | Journal of Landscape Research2013，5，9： | 1 |
| 18 | Chronological Constraints on Late Paleozoic Collision in the Southwest Tianshan Orogenic Belt, China: Evidence from the Baleigong Granites显示文摘The Baleigong granites, located in the western part of the southwestern Tianshan Orogen(Kokshanyan region, China), records late Paleozoic magmatism during the late stages of convergence between the Tarim Block and the Central Tianshan Arc Terrane. We performed a detailed geochronological and geochemical study of the Baleigong granites to better constrain the nature of collisional processes in the Southwest Tianshan Orogen. The LA-ICP-MS U-Pb zircon isotopic analyses indicate that magmatism commenced in the early Permian(~282 Ma). The granite samples, which are characterized by high contents of SiO2(67.68-69.77 wt%) and Al2O3(13.93-14.76 wt%), are alkali-rich and Mg-poor, corresponding to the high-K calc-alkaline series. The aluminum saturation index(A/CNK) ranges from 0.93 to 1.02, indicating a metaluminous to slightly peraluminous composition. Trace element geochemistry shows depletions in Nb, Ta, and Ti, a moderately negative Eu anomaly(δEu=0.40-0.56), enrichment in LREE, and depletion in HREE((La/Yb)N=7.46-11.78). These geochemical signatures are characteristic of an I-type granite generated from partial melting of a magmatic arc. The I-type nature of the Baleigong granites is also supported by the main mafic minerals being Fe-rich calcic hornblende and biotite. We suggest that the high-K, calc-alkaline I-type granitic magmatism was generated by partial melting of the continental crust, possibly triggered by underplating by basaltic magma. These conditions were likely achieved in a collisional tectonic setting, thus supporting the suggestion that closure of the South Tianshan Ocean was completed prior to the Permian and was followed(in the late Paleozoic) by collision between the Tarim Block and the Central Tianshan Arc Terrane. | HUO Hailong CHEN Zhengle ZHANG Qing HAN Fengbin ZHANG Wengao SUN Yue YANG Bin TANG Yanwen | 2019 | Acta Geologica Sinica(English Edition)2019，93，5： | 1 |
| 19 | Timed-pNets: a communication behavioural semantic model for distributed systems显示文摘 | Yanwen CHEN Yixiang CHEN Eric MADELAINE | 2015 | Frontiers of Computer Science2015，9，1： | 1 |
| 20 | In vivo assessment of hepatotoxicity,nephrotoxicity and biodistribution using 3-aminopropyltriethoxysilane-coated magnetic nanoparticles(APTS-MNPs) in ICR mice显示文摘The biocompatibility and biodistribution of magnetic nanoparticles(MNPs)in vivo are essential to ensure their safely clinical application.We have studied these aspects with our 3-aminopropyltriethoxysilanecoated magnetic nanoparticles(APTS-MNPs)formulation,which can be used as magnetic induction hyperthermia media.Changes in tissue iron levels were analyzed after intraperitoneal injection of APTS-MNPs to ICR mice.Liver and kidney functions were tested.Heart,liver,spleen,lung,kidney,testis,and brain were sectioned for pathological analysis.Biodistribution of iron in various body tissues changed with time but greater fraction of the injected iron localized in the liver and spleen than in other tissues.Serum showed an increase in AST and LDH following APTS-MNPs injection.Histological analyses of selected tissues showed no obvious abnormal changes.In conclusion,APTS-MNPs did not cause continuing changes in the liver and kidney function and thus can be safely used for in vivo application. | Xiaowen Wang Jieying Zhang Xin Yang Zhenghai Tang Yanwen Hu Benke Chen Jintian Tang | 2014 | Chinese Science Bulletin2014，59，16： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员