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| 1 | Generation of B Cell-Deficient Pigs by Highly Efficient CRISPR/Cas9-Mediated Gene Targeting显示文摘Generating B cell-deficient mutant is the first step to produce human antibody repertoires in large animal models. In this study, we applied the clustered regularly interspaced short palindromic repeat(CRISPR)/CRISPR-associated(Cas) system to target the JHregion of the pig Ig M heavy chain gene which is crucial for B cell development and differentiation. Transfection of Ig M-targeting Cas9 plasmid in primary porcine fetal fibroblasts(PFFs) enabled inducing gene knock out(KO) in up to 53.3% of colonies analyzed, a quarter of which harbored biallelic modification, which was much higher than that of the traditional homologous recombination(HR). With the aid of somatic cell nuclear transfer(SCNT) technology, three piglets with the biallelic Ig M heavy chain gene mutation were produced. The piglets showed no antibody-producing B cells which indicated that the biallelic mutation of the Ig M heavy chain gene effectively knocked out the function of the Ig M and resulted in a B cell-deficient phenotype. Our study suggests that the CRISPR/Cas9 system combined with SCNT technology is an efficient genome-editing approach in pigs. | Fengjiao Chen Ying Wang Yilin Yuan Wei Zhang Zijian Ren Yong Jin Xiaorui Liu Qiang Xiong Qin Chen Manling Zhang Xiaokang Li Lihua Zhao Ze Li Zhaoqiang Wu Yanfei Zhang Feifei Hu Juan Huang Rongfeng Li Yifan Dai | 2015 | Journal of Genetics and Genomics2015，42，8： | 15 |
| 2 | “Exploring petroleum inside source kitchen”: Shale oil and gas in Sichuan Basin显示文摘The Sichuan Basin is rich in shale oil and gas resources,with favorable geological conditions that the other shale reservoirs in China cannot match.Thus,the basin is an ideal option for fully'exploring petroleum inside source kitchen'with respect to onshore shale oil and gas in China.This paper analyzes the characteristics of shale oil and gas resources in the United States and China,and points out that maturity plays an important role in controlling shale oil and gas composition.US shale oil and gas exhibit high proportions of light hydrocarbon and wet gas,whereas Chinese marine and transitional shale gas is mainly dry gas and continental shale oil is generally heavy.A comprehensive geological study of shale oil and gas in the Sichuan Basin reveals findings with respect to the following three aspects.First,there are multiple sets of organic-rich shale reservoirs of three types in the basin,such as the Cambrian Qiongzhusi Formation and Ordovician Wufeng Formation-Silurian Longmaxi Formation marine shale,Permian Longtan Formation transitional shale,Triassic Xujiahe Formation lake-swamp shale,and Jurassic lacustrine shale.Marine shale gas enrichment is mainly controlled by four elements:Deep-water shelf facies,moderate thermal evolution,calcium-rich and silicon-rich rock association,and closed roof/floor.Second,the'sweet section'is generally characterized by high total organic carbon,high gas content,large porosity,high brittle minerals content,high formation pressure,and the presence of lamellation/bedding and natural microfractures.Moreover,the'sweet area'is generally characterized by very thick organic-rich shale,moderate thermal evolution,good preservation conditions,and shallow burial depth,which are exemplified by the shale oil and gas in the Wufeng-Longmaxi Formation,Longtan Formation,and Daanzhai Member of the Ziliujing Formation.Third,the marine,transitional,and continental shale oil and gas resources in the Sichuan Basin account for 50%,25%,and 30%of the respective types of shale oil and gas geological resources in China,with great potential to become the cradle of the shale oil and gas industrial revolution in China.Following the'Conventional Daqing-Oil'(i.e.,the Daqing oilfield in the Songliao Basin)and the'Western Daqing-Oil&Gas'(i.e.,the Changqing oilfield in the Ordos Basin),the Southwest oil and gas field in the Sichuan Basin is expected to be built into a'Sichuan-Chongqing Daqing-Gas'in China. | Caineng ZOU Zhi YANG Shasha SUN Qun ZHAO Wenhua BAI Honglin LIU Songqi PAN Songtao WU Yilin YUAN | 2020 | Science China Earth Sciences2020，63，7： | 6 |
| 3 | Precise in vivo genome editing via single homology arm donor mediated intron-targeting gene integration for genetic disease correction显示文摘In vivo genome editing represents a powerful strategy for both understanding basic biology and treating inherited diseases.However,it remains a challenge to develop universal and efficient in vivo genome-editing tools for tissues that comprise diverse cell types in either a dividing or non-dividing state.Here,we describe a versatile in vivo gene knock-in methodology that enables the targeting of a broad range of mutations and cell types through the insertion of a minigene at an intron of the target gene locus using an intracellularly linearized single homology arm donor.As a proof-of-concept,we focused on a mouse model of prematureaging caused by a dominant point mutation,which is difficult to repair using existing in vivo genome-editing tools.Systemic treatment using our new method ameliorated aging-associated phenotypes and extended animal lifespan,thus highlighting the potential of this methodology for a broad range of in vivo genome-editing applications. | Keiichiro Suzuki Mako Yamamoto Reyna Hernandez-Benitez Zhe Li Christopher Wei Rupa Devi Soligalla Emi Aizawa Fumiyuki Hatanaka Masakazu Kurita Pradeep Reddy Alejandro Ocampo Tomoaki Hishida Masahiro Sakurai Amy NNemeth Estrella Nunez Delicado Josep MCampistol Pierre Magistretti Pedro Guillen Concepcion Rodriguez Esteban Jianhui Gong Yilin Yuan Ying Gu Guang-Hui Liu Carlos Lopez-Otin Jun Wu Kun Zhang Juan Carlos Izpisua Belmonte | 2019 | Cell Research2019，29，10： | 2 |
| 4 | Design of antibacterial peptide-like conjugated molecule with broad spectrum antimicrobial ability显示文摘Increasing multidrug-resistant(MDR) superbugs emerge worldwide causing a public health crisis. Consequently, it is urgent to find new antibiotics with efficient broad-spectrum antimicrobial activity. By virtue of versatility in molecular design, a new peptide-like cell membrane-broken molecule, oligo-(7,7′-bifluoren-benzo[c][1,2,5]thiadiazole)(OFBT) possessing a conjugated backbone and eight pendant guanidyl moieties was designed and synthesized. OFBT exhibits favorable broad-spectrum of antimicrobial activity to pathogens including Gram-negative and Gram-positive bacteria, and fungi with the minimum inhibitory concentration(MIC)below 3.0 μM. Moreover, OFBT exhibits high selectivity for pathogens over human cells to make it a promising broad spectrum antimicrobial agent. | Hui Chen Meng Li Zhang Liu Rong Hu Shengliang Li Yuan Guo Fengting Lv Libing Liu Yilin Wang Yuanping Yi Shu Wang | 2018 | Science China Chemistry2018，61，1： | 1 |
| 5 | Tumor microenvironment-responsive modular integrated nanocomposites for magnetically targeted and photothermal enhanced catalytic therapy显示文摘Achieving efficient integration of cancer diagnosis and therapy is of great significance to human health,but the construction of a multifunctional intelligent therapy system still faces great challenges.In this study,we report an integrated multifunctional nanocomposite constructed by a simple modular assembly technology.The nanocomposites are composed of three different nanomaterials:Fe_(3)O_(4),Au,and NaErF_(4):0.5%Tm@NaYF_(4)upconversion nanoparticles(UCNPs).In this design,Fe_(3)O_(4)nanoparticles have nanozyme effect of peroxidase-like activity,which can react with H_(2)O_(2)in the tumor microenvironment to generate hydroxyl radicals.Because of its magnetic properties,it can help the nanocomposites to aggregate under the induction of magnetic fields.Au nanoparticles exhibit nanozyme effect of glucose oxidase-like activity.It can catalyze the conversion of glucose to gluconic acid and H_(2)O_(2).Ingeniously,the generated H_(2)O_(2)provides a source of reactants for the reaction of the Fe_(3)O_(4)nanozyme.In addition,the photothermal effect of Au nanoparticles under 808 nm irradiation further enhanced the nanozyme activity of Fe_(3)O_(4)and Au nanoparticles.Besides,UCNPs can emit near-infrared(NIR)-II fluorescence under 808 nm irradiation,which can provide imaging-guided during cancer treatment.Then,the nanocomposites were further modified by poly(vinylpyrrolidone)(PVP)to obtain UCNPs/Au/Fe_(3)O_(4)-PVP with good biocompatibility and high-efficiency cancer treatment ability. | Yuan Liang Yilin Liu Pengpeng Lei Zhen Zhang Hongjie Zhang | 2023 | Nano Research2023，16，7： | 1 |
| 6 | Model-based joint bit allocation between texture videosand depth maps for 3-D video coding显示文摘 | YUAN Hui CHANG Yilin HUO Junyan | 2011 | IEEE Trans-actions on Circuits and Systems for Video Technology2011，21，4： | 1 |
| 7 | Melatonin modulates the effects of diethylstilbestrol (DES) on the anterior pituitary of the female Wistar rat显示文摘 | Zhao Weijiang Shi Zhongfang Yuan Fang Li Guilin Sun Yilin Zhang Yazhuo Wang Zhongcheng | 2010 | Folia Histochemica et Cytobiologica2010，，2： | 1 |
| 8 | Heterojunction interface editing in Co/NiCoP nanospheres by oxygen atoms decoration for synergistic accelerating hydrogen and oxygen evolution electrocatalysis显示文摘Controllable designing of well-defined heterojunction nanostructures provides an insightful strategy for accelerating the kinetics of the hydrogen and oxygen evolution reactions(HER/OER),but such task is still challenging.Herein,we proposed a protocol of heterojunction interface editing(HIE)strategy by oxygen atoms decoration for synergistic boosting electrocatalytic HER and OER performances.A novel Co/NiCoP nanospheres(NSs)heterojunction was synthesized by crystal seed template transformation method with Ni_(5)P_(4) microspheres as seeds.The effective oxygen atoms interface editing increased the oxidation state of Co atoms and prolonged the Co-P bond length of Co/NiCoP NSs heterojunction,thus the electron localization on P sites was enhanced,leading to the dramatically elevated HER and OER performances simultaneously.The as-constructed O-Co/NiCoP NSs show excellent electrocatalytic activity with 361 and 430 mV vs.reversible hydrogen electrode(RHE)to arrive high current density of 300 mA·cm^(-2)for HER and OER in 1 M KOH as well as good stability.The proposed HIE concept could provide a new perspective on the catalyst design for energy conversion systems. | Yan Lin Xiaojiao Cui Yilin Zhao Zhicheng Liu Guoxin Zhang Yuan Pan | 2023 | Nano Research2023，16，7： | 1 |
| 9 | Mesenchymal stromal cells attenuate post-stroke infection by preventing caspase-1-dependent splenic marginal zone B cell death显示文摘Dear Editor,Spontaneous infection is one of the most common complications of acute ischemic stroke(AIS)and leads to increased morbidity and mortality in stroke patients.However,current interventions fail to improve clinical outcomes in these patients.Since stroke-induced immunodeficiency is thought to contribute to the risk of infection,1 we hypothesized that mesenchymal stromal cell(MSC)therapy,which harnesses potent immunomodulatory capacities,could be a potential solution.Here,we show that intravenously administered MSCs preferentially migrate to the marginal zone(MZ)of the spleen,preserve the injured MZ B cells and ameliorate post-stroke infection and mortality in the mouse middle cerebral artery occlusion(MCAO)model of AIS. | Yinong Huang Jiancheng Wang Xiaofan Lai Yuan Qiu Jianye Cai Yuanchen Ma Yilin Liu Wei Qiu Zhengqi Lu Andy Peng Xiang | 2021 | Signal Transduction and Targeted Therapy2021，6，3： | 1 |
| 10 | Electrochemical migration failure of the copper trace on printed circuit board driven by immersion silver finish显示文摘 | Zhou Yilin Yang Pan Yuan Chengming | 2013 | Chemical Engineering Transaction2013，33，： | 1 |
| 11 | Status and trend of hydrogeological experiments concerning the side-wall flow effect in porous media显示文摘Hydrogeological experiments provide an important means to understand groundwater seepage and solute transport problems.Basic hydrogeological parameters can be obtained for use in assessing groundwater resources.In current porous-media research,studies of side-wall flow are in a preliminary and qualitative phase,and lack systematic and comprehensive understanding.Side-wall flow refers to the non-uniform infiltration and solute transport that occurs near the seepage device boundary.Flow dynamics depends on the fluid under the specific hydraulic conditions,physical properties and chemical composition,medium permeability,the roughness of the side wall,geometric features,and physical chemistry.Such phenomena not only occur in indoor water flows and solute transport,but also under natural conditions in the field.Side-wall flow has features both in common as well as distinct from preferential flows.In porous-media experiments,once side-wall flow commences,it affects the groundwater flow field and chemistry field,resulting in parameter values deviating from actual values.Based on a comprehensive analysis of the influence of side-wall flow,a definition of the side-wall flow effect in porous media is given.Three directions of research are identified concerning side-wall flow:the mechanism of the side-wall flows effect,the study of its quantitative impact on seepage flow and solute transport,and the methods and measures that need to be taken in hydrogeological experiments to reduce(or prevent)side-wall flow development. | Fugang Wang Zhenkai Gao Yilin Yuan Jing Jing Xinxin Geng Yuqing Cao | 2014 | Chinese Science Bulletin2014，59，8： | 1 |
| 12 | Security for cyber-physical systems: Secure control against known-plaintext attack显示文摘There has been a surge of interests in the security of cyber-physical systems(CPSs), yet it is commonly assumed that the adversary has a full knowledge of physical system models. This paper argues that such an unrealistic assumption can be relaxed: the adversary might still be able to identify the system model by passively observing the control input and sensory data. In such a setup, the attack with knowledge of input-output data can be categorized as a Known-Plaintext Attack. A necessary and sufficient condition has been provided, under which the adversary can uniquely obtain the knowledge of the underlying physical system.From the defender's perspective, a secure controller design—which exhibits a low rank structure—is proposed which renders the system unidentifiable to the adversary, while trading off the control system's performance. Finally, a numerical example has been provided to demonstrate the effectiveness of the proposed secure controller design. | YUAN Ye MO YiLin | 2020 | Science China(Technological Sciences)2020，63，9： | 0 |
| 13 | Highly Efficient Self-Trapped Bluish White-Light Emission from[Pb4Cl5]3+Nodes in a Moisture-Tolerant Metal–Organic Framework显示文摘Highly luminescent zero-dimensional(0D)metal halide clusters attract widespread attention owing to strong exciton confinement and populated self-trapped states but often exhibit narrow emission and are susceptible to hydrolysis.Herein,we demonstrate a moisture-resistant metal–organic framework(MOF)consisting of cationic 0D[Pb_(4)Cl_(5)]^(3+)nodes bridged by adamantanetetracarboxylate.Upon near-UV excitation,the material emits intrinsic broadband bluish white-light emission with high external quantum efficiency of 35%and a color rendering index of 76.Unlike organoammonium cations in lead perovskites,the Pb-carboxylate coordination affords the MOF to be chemically stable and photostable in high humidity.The photoemitter exhibits undiminished photoemissions under ambient conditions[∼60%relative humidity(RH)]upon continuous UV irradiation(143 mW/cm^(2),365 nm)for 7 days.The insertion of[Na_(4)Cl]^(3+)moieties will connect 0D units into two-dimensional(2D)metal halide layers to limit structural strain and decrease the quantum efficiency from 35%to 15%,confirming the key importance of 0D units for efficient emission. | Jinlin Yin Yuan Yu Xueling Song Yilin Jiang Honghan Fei | 2022 | CCS Chemistry2022，4，2： | 0 |
| 14 | Rosa chinensis as edible flowers:phytochemicals and biological effects显示文摘Rosa chinensis as edible flowers,is a kind of traditional Chinese medicine,which is rich in resources and is cultivated in most parts of China.Its phytochemicals mainly include flavonoids and their glycosides,phenolic acids,pentacyclic triterpenes and steroids.R.chinensis has many biological activities,including antioxidation,antibiosis,protection of pancreatic islet cells.The phytochemicals and biological activities of R.chinensis were summarized by databases such as Web of Science,SciFinder,Baidu Scholar,PubMed,ScienceDirect and SpringerLink. | Huihui Zhou Ming Liu Yilin Yuan Nashwa M.Shalapy Lili Cui | 2023 | Journal of Future Foods2023，3，4： | 0 |
| 15 | Single-atomic Mn sites coupled with Fe_(3)C nanoparticles encapsulated in carbon matrixes derived from bimetallic Mn/Fe polyphthalocyanine conjugated polymer networks for accelerating electrocatalytic oxygen reduction显示文摘The construction of robust coupling catalysts for accelerating electrocatalytic oxygen reduction reaction(ORR)through the modulation of the electronic structure and local atomic configuration is critical but remains challenging.Herein,we report a facile and effective isolation-polymerization-pyrolysis(IPP)strategy for high-precision synthesis of single-atomic Mn sites coupled with Fe_(3)C nanoparticles encapsulated in N-doped porous carbon matrixes(Mn SAs/Fe_(3)C NPs@NPC)catalyst derived from predesigned bimetallic Fe/Mn polyphthalocyanine(FeMn-BPPc)conjugated polymer networks by solid-phase reaction approach.Benefiting from the synergistic effects between the single-atomic Mn-N_(4)sites and Fe_(3)C NPs as well as the confinement effect of NPC,the Mn SAs/Fe_(3)C NPs@NPC catalyst exhibited excellent electrocatalytic activity and stability for ORR.The assembled Znair battery displayed larger power density of 186 mW·cm^(−2)than that of Pt/C+Ir/C-based battery.It also exhibits excellent stability without obvious voltage change after 106 cycles with 36 h.Combing in-situ Raman spectra with in-situ attenuated total reflectance surface-enhanced infrared absorption spectroscopy(ATR-SEIRAS)characterization results indicated that the Mn-N_(4)site as an active site for the O_(2)adsorption-activation process,which effectively facilitates the generation of key*OOH intermediates and*OH desorption to promote the multielectron reaction kinetics.Theoretical calculation reveals that the excellent electrocatalytic performance originates from the charge redistribution and the d orbital shift resulting from Mn-Fe bond,which buffers the activity of ORR through the electron reservoir capable of electron donation or releasing.This work paves a novel IPP strategy for constructing high-performance coupling electrocatalyst towards the ORR for energy conversion devices. | Yuan Pan Min Li Wanliang Mi Minmin Wang Junxi Li Yilin Zhao Xuelu Ma Bin Wang Wei Zhu Zhiming Cui Hailiang Yin Yunqi Liu | 2022 | Nano Research2022，15，9： | 0 |
| 16 | Powdery mildew disease resistance and markerassisted screening at the Pm60 locus in wild diploid wheat Triticum urartu显示文摘Triticum urartu,a diploid wild wheat and progenitor of the A genome of bread wheat,is an important resource for resistance to powdery mildew fungus caused by Blumeria graminis f.sp.tritici(Bgt).In the present study we systematically characterized the interaction between the Bgt fungus and T.urartu at the microscopic level.We also tested 227 T.urartu accessions for reaction to Bgt isolate E09 and discovered previously uncategorized powdery mildew resistance in this collection.Pm60 is a CC-NB-LRR type powdery mildew resistance gene that has at least three functional alleles,Pm60,Pm60a,and Pm60b.A marker-assisted screen targeting the Pm60 locus identified a non-functional allele of Pm60a,designated as Pm60a′.A sequence comparison of Pm60a′and Pm60a revealed that they differed by 58 SNPs and one 3-nucleotide deletion.Based on the sequence variations two molecular markers were developed to differentiate the functional Pm60a allele from the non-functional Pm60a′.Our screen revealed the presence of a previously uncharacterized powdery mildew resistance in T.urartu and provides new insights into the Pm60 locus.We believe that the two molecular markers developed here and new T.urartu resistant accessions will facilitate further identification of novel powdery mildew resistance genes and benefit breeding for powdery mildew resistance. | Fukai Zhao Yinghui Li Baoju Yang Hongbo Yuan Cong Jin Lixun Zhou Hongcui Pei Lifang Zhao Yiwen Li Yilin Zhou Jiankun Xie Qian-Hua Shen | 2020 | The Crop Journal2020，8，2： | 0 |
| 17 | Motion feature descriptor based moving objects segmentation显示文摘 | Yuan Hui Chang Yilin Ma Yanzhuo Bai Donglin Lu Zhaoyang | 2012 | High Technology Letters2012，18，1： | 0 |
| 18 | Temporal compressive super-resolution microscopy at frame rate of 1200 frames per second and spatial resolution of 100 nm显示文摘Various super-resolution microscopy techniques have been presented to explore fine structures of biological specimens.However,the super-resolution capability is often achieved at the expense of reducing imaging speed by either point scanning or multiframe computation.The contradiction between spatial resolution and imaging speed seriously hampers the observation of high-speed dynamics of fine structures.To overcome this contradiction,here we propose and demonstrate a temporal compressive super-resolution microscopy(TCSRM)technique.This technique is to merge an enhanced temporal compressive microscopy and a deep-learning-based super-resolution image reconstruction,where the enhanced temporal compressive microscopy is utilized to improve the imaging speed,and the deep-learning-based super-resolution image reconstruction is used to realize the resolution enhancement.The high-speed super-resolution imaging ability of TCSRM with a frame rate of 1200 frames per second(fps)and spatial resolution of 100 nm is experimentally demonstrated by capturing the flowing fluorescent beads in microfluidic chip.Given the outstanding imaging performance with high-speed super-resolution,TCSRM provides a desired tool for the studies of high-speed dynamical behaviors in fine structures,especially in the biomedical field. | Yilin He Yunhua Yao Dalong Qi Yu He Zhengqi Huang Pengpeng Ding Chengzhi Jin Chonglei Zhang Lianzhong Deng Kebin Shi Zhenrong Sun Xiaocong Yuan Shian Zhang | 2023 | Advanced Photonics2023，5，2： | 0 |
| 19 | Generation of monoclonal antibodies against n-3 fatty acid desaturase显示文摘Dear Editor:Omega-3 polyunsaturated fatty acids(n-3 PUFAs)are essential fatty acids for normal cellular functions and have been used for prevention and treatment of many diseases,including coronary heart disease,diabetes,and cancers[1-3].n-3 PUFAs and n-6 PUFAs have been shown to decrease and increase the severity of several human diseases,respectively[4].Unfortunately,mammals have no enzymes to synthesize n-6 and | Yanfei Zhang Yilin Yuan Meilei Sheng Wei Zhang Ying Wang Xiao Zhang Yifan Dai | 2015 | The Journal of Biomedical Research2015，29，6： | 0 |
| 20 | Construction of N-doped carbon frames anchored with Co single atoms and Co nanoparticles as robust electrocatalyst for hydrogen evolution in the entire pH range显示文摘The development of low-cost, efficient, and high atomic economy electrocatalysts for hydrogen evolution reaction(HER) in the entire p H range for sustainable hydrogen production is of great importance but still challenging. Herein, we synthesize a highly dispersed N-doped carbon frames(NCFs) anchored with Co single atoms(SAs) and Co nanoparticles(NPs) catalyst by a doping-adsorption-pyrolysis strategy for electrocatalytic hydrogen evolution. The Co SAs-Co NPs/NCFs catalyst exhibits an excellent HER activity with small overpotential, low Tafel slope, high turnover frequency as well as remarkable stability. It also exhibits a superior HER performance in the entire p H range. Combining with experimental and theoretical calculation, we find that Co SAs with Co-N_(3) coordination structure and Co NPs have a strong interaction for promoting synergistic HER electrocatalytic process. The H_(2)O molecule is easily activated and dissociated on Co NPs, while the generated H^(*) is easily adsorbed on Co SAs for HER, which makes the Co SAs-Co NPs/NCFs catalyst exhibit more suitable H adsorption strength and more conducive to the activation and dissociation of H_(2)O molecules. This work not only proposes a novel idea for constructing coupling catalyst with atomic-level precision, but also provides strong reference for the development of high-efficiency HER electrocatalysts for practical application. | Minmin Wang Min Li Yilin Zhao Naiyou Shi Hui Zhang Yuxue Zhao Yaru Zhang Haoran Zhang Wenhong Wang Kaian Sun Yuan Pan Shoujie Liu Houyu Zhu Wenyue Guo Yanpeng Li Yunqi Liu Chenguang Liu | 2022 | Journal of Energy Chemistry2022，31，4： | 0 |
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