|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Efficient and Specific Modifications of the Drosophila Genome by Means of an Easy TALEN Strategy显示文摘技术开发总是是在生物医学的研究驾驶突破的力量之一。自从托马斯·摩根的时间起,一步一步地, Drosophilists 为操作并且机能上地把开发了强大的基因工具为改进这些技术并且开发新技术的果蝇染色体,而是房间仍然大,今天特别作为生物学家开始系统地学习不同模型有机体的功能的 genomics ,包括人,以一种高产量的方式。这里,我们报导,第一次在果蝇,为借助于改进抄写在很高的效率修改果蝇染色体的一个快速、容易、高度特定的方法像使活跃之物的受动器核酸酶(TALEN ) 策略。我们利用了最近发达的单位集会策略装配设计修改黄基因的二特定的 TALEN (在性染色体上) 并且新奇正染色体的基因。TALEN 的 mRNAs 随后被注入果蝇胚胎。从 31.2% 注射 F0 肥沃的苍蝇,我们检测了包含黄基因的可继承的修正。从到可继承的修正的察觉的特定的 TALEN 的建设的全部过程能在一个月以内被完成。在果蝇的这个调停 TALEN 的染色体修正方法的潜在的应用程序被讨论。 | Jiyong Liu Changqing Li Zhongsheng Yu Peng Huang Honggang Wu Chuanxian Wei Nannan Zhu Yan Shen Yixu Chen Bo Zhang Wu-Min Deng Renjie Jiao | 2012 | Journal of Genetics and Genomics2012，39，5： | 43 |
| 2 | Highly efficient base editing in human tripronuclear zygotes显示文摘 | Changyang Zhou Meiling Zhang Yu Wei Yidi Sun Yun Sun Hong Pan Ning Yao Wanxia Zhong Yixue Li Weiping Li3 Hui Yang Zi-jiang Chen | 2017 | Protein & Cell2017，8，10： | 6 |
| 3 | Drosophila RecQ5 is required for efficient SSA repair and suppression of LOH in vivo显示文摘RecQ5 in mammalian cells has been suggested to suppress inappropriate homologous recombination.However,the specific pathway(s)in which it is involved and the underlining mechanism(s)remain poorly understood.We took advantage of genetic tools in Drosophila to investigate how Drosophila RecQ5(dRecQ5)functions in vivo in homologous recombination-mediated double strand break(DSB)repair.We generated null alleles of dRecQ5 using the targeted recombination technique.The mutant animals are homozygous viable,but with growth retardation during development.The mutants are sensitive to both exogenous DSB-inducing treatment,such as gamma-irradiation,and endogenously induced double strand breaks(DSBs)by I-Sce I endonuclease.In the absence of dRecQ5,single strand annealing(SSA)-mediated DSB repair is compromised with compensatory increases in either inter-homologous gene conversion,or non-homologous end joining(NHEJ)when inter-chromosomal homologous sequence is unavailable.Loss of function of dRecQ5 also leads to genome instability in loss of heterozygosity(LOH)assays.Together,our data demonstrate that dRecQ5 functions in SSA-mediated DSB repair to achieve its full efficiency and in suppression of LOH in Drosophila. | Yixu Chen Wen Dui Zhongsheng Yu Changqing Li Jun Ma Renjie Jiao | 2010 | Protein & Cell2010，1，5： | 5 |
| 4 | Big Biological Data:Challenges and Opportunities显示文摘In‘‘Omics’’era of the life sciences,data is presented in many forms,which represent the information at various levels of bio logical systems,including data about genome,transcriptome epigenome,proteome,metabolome,molecular imaging,molec ular pathways,different population of people and clinical/med ical records.The biological data is big,and its scale has already been well beyond petabyte(PB)even exabyte(EB). | Yixue Li Luonan Chen | 2014 | Genomics, Proteomics & Bioinformatics2014，12，5： | 3 |
| 5 | Overview of SMP-CAIL2020-Argmine:The Interactive Argument-Pair Extraction in Judgement Document Challenge显示文摘In this paper we present the results of the Interactive Argument-Pair Extraction in Judgement Document Challenge held by both the Chinese AI and Law Challenge(CAIL)and the Chinese National Social Media Processing Conference(SMP),and introduce the related data set-SMP-CAIL2020-Argmine.The task challenged participants to choose the correct argument among five candidates proposed by the defense to refute or acknowledge the given argument made by the plaintiff,providing the full context recorded in the judgement documents of both parties.We received entries from 63 competing teams,38 of which scored higher than the provided baseline model(BERT)in the first phase and entered the second phase.The best performing system in the two phases achieved accuracy of 0.856 and 0.905,respectively.In this paper,we will present the results of the competition and a summary of the systems,highlighting commonalities and innovations among participating systems.The SMP-CAIL2020-Argmine data set and baseline modelshave been already released. | Jian Yuan Zhongyu Wei Yixu Gao Wei Chen Yun Song Donghua Zhao Jinglei Ma Zhen Hu Shaokun Zou Donghai Li Xuanjing Huang | 2021 | Data Intelligence2021，3，2： | 3 |
| 6 | Analysis of the origin of peak aerosol optical depth in springtime over the Gulf of Tonkin显示文摘By aggregating MODIS(moderate-resolution imaging spectroradiometer) AOD(aerosol optical depth) and OMI(ozone monitoring instrument) UVAI(ultra violet aerosol index)datasets over 2010–2014, it was found that peak aerosol loading in seasonal variation occurred annually in spring over the Gulf of Tonkin(17–23°N, 105–110°E). The vertical structure of the aerosol extinction coefficient retrieved from the spaceborne lidar CALIOP(cloud-aerosol lidar with orthogonal polarization) showed that the springtime peak AOD could be attributed to an abrupt increase in aerosol loading between altitudes of 2 and 5 km.In contrast, aerosol loading in the low atmosphere(below 1 km) was only half of that in winter. Wind fields in the low and high atmosphere exhibited opposite transportation patterns in spring over the Gulf of Tonkin, implying different sources for each level. By comparing the emission inventory of anthropogenic sources with biomass burning, and analyzing the seasonal variation of the vertical structure of aerosols over the Northern Indo-China Peninsula(NIC), it was concluded that biomass burning emissions contributed to high aerosol loading in spring. The relatively high topography and the high surface temperature in spring made planetary boundary layer height greater than 3 km over NIC. In addition, small-scale cumulus convection frequently occurred, facilitating pollutant rising to over 3 km, which was a height favoring long-range transport. Thus, pollutants emitted from biomass burning over NIC in spring were raised to the high atmosphere, then experienced long-range transport, leading to the increase in aerosol loading at high altitudes over the Gulf of Tonkin during spring. | Xiaoli Shan Jun Xu Yixue Li Feng Han Xiaohui Du Jingying Mao Yunbo Chen Youjiang He Fan Meng Xuezhi Dai | 2016 | Journal of Environmental Sciences2016，28，2： | 2 |
| 7 | MKRN3 regulates the epigenetic switch of mammalian puberty via ubiquitination of MBD3显示文摘Central precocious puberty(CPP)refers to a human syndrome of early puberty initiation with characteristic increase in hypothalamic production and release of gonadotropin-releasing hormone(GnRH).Previously,loss-of-function mutations in human MKRN3,encoding a putative E3 ubiquitin ligase,were found to contribute to about 30%of cases of familial CPP.MKRN3 was thereby suggested to serve as a‘brake’of mammalian puberty onset,but the underlying mechanisms remain as yet unknown.Here,we report that genetic ablation of Mkrn3 did accelerate mouse puberty onset with increased production of hypothalamic GnRH1.MKRN3 interacts with and ubiquitinates MBD3,which epigenetically silences GNRH1 through disrupting the MBD3 binding to the GNRH1 promoter and recruitment of DNA demethylase TET2.Our findings have thus delineated a molecular mechanism through which the MKRN3-MBD3 axis controls the epigenetic switch in the onset of mammalian puberty. | Chuanyin Li Wenli Lu Liguang Yang Zhengwei Li Xiaoyi Zhou Rong Guo Junqi Wang Zhebao Wu Zhiya Dong Guang Ning Yujiang Shi Yinmin Gu Peng Chen Zijian Hao Tianting Han Meiqiang Yang Wei Wang Xuehui Huang Yixue Li Shan Gao Ronggui Hu | 2020 | National Science Review2020，7，3： | 2 |
| 8 | Study of irradiation effects in China low activation martensitic steel CLAM显示文摘 | Qunying Huang Jiangang Li Yixue Chen | 2004 | Journal of Nuclear Materials2004，，： | 1 |
| 9 | Smart ZnS@C filler for super-anticorrosive self-healing zinc-rich epoxy coating显示文摘The zinc-rich epoxy cathodic protection coating is the most widely used anticorrosion material for marine steel.However,traditional conductive fillers lack the intelligent self-healing effect,which limits the long-term anticorrosion performance.Herein,with uniform carbon-coated ZnS(ZnS@C)nanoballs as the smart active release filler,we propose an anticorrosive and self-healing zinc-rich maleic anhydride epoxy coating.Due to the high pore filling efficiency of the nanoballs,the water vapor transmission rate of the coating with an initial corrosion efficiency of 99.92%and a low-frequency impedance of|Z|f=10mHz=3.88×10^(10) Ω·cm^(2),was reduced by 52%.The carbon-shell of the nanoball increases electron transmission paths in the coating and improves conductivity by nearly two orders of magnitude,which effectively activates more Zn-sites and extends the cathodic protection time.Moreover,once the steel-substrate undergoes regional corrosion,the SO_(4)^(2-)hydrolyzes from the ZnS-core of the nanoball and reacts with iron ions on the corroded area accurately and intelligently to fill the gap and self-heals into a new dense barrier layer(Fe_(2)(SO_(4))_(3),etc.),which significantly improves the shielding protection ability during the long-term usage of the coating.The effective anticorrosion time of the proposed coating could be up to 3,400 h. | Kai Yang Yixue Duan Guicheng Liu Guoyan Ma Hao Fu Xuyong Chen Manxiang Wang Gangqiang Zhu Woochul Yang Yiding Shen | 2022 | Nano Research2022，15，5： | 1 |
| 10 | Predicting protein-protein interactions based only on sequences information显示文摘 | SHEN Juwen ZHANG Jian LuO Xiaomin ZHU Weiliang Yu Kunqian CHEN Kaixian LI Yixue JIANG Hualiang | 2007 | Proceedings of the National Academy of Sciences2007，104，11： | 1 |
| 11 | Adsorption of Sr (Ⅱ) and Eu (Ⅲ) on Na-rectorite: Ef- fect of pH, Ionic Strength, Concentration and Modelling显示文摘 | Hu Jun Chen CL Sheng GD Li Jiaxing Chen Yixue Wang Xiangke | 2010 | Radiochimica Acta2010，98，7： | 1 |
| 12 | Monte Carlo based operational and shutdown dose rate ealeulations of HT-7U tokamak 显示文摘 | Yixue Chen Yiean Wu Qunying Huang | 2004 | Fusion Engineering and Design2004，70，2004： | 1 |
| 13 | Diffusion of Eu(III) in compacted bentonite—effect of pH, solution concentration and humic acid显示文摘 | Xiangke Wang Yixue Chen Yican Wu | 2004 | Applied Radiation and Isotopes2004，，6： | 1 |
| 14 | Diffusion of Eu (Ⅲ) in Compacted Bentonite-Effect of pH, Solution Concentration and Humic Acid 显示文摘 | Wang Xiangke Chen Yixue Wu Yican | 2004 | Appl Radiat Isot2004，60，： | 1 |
| 15 | Using of chelating resin to study the kinetic desorption of Eu(III) from humic acid–Al 2 O 3 colloid surfaces显示文摘 | Xiangke Wang Xiang Zhou Jinzhou Du Wenping Hu Changlun Chen Yixue Chen | 2005 | Surface Science2005，，2： | 1 |
| 16 | Kinase-substrate Edge Biomarkers Provide a More Accurate Prognostic Prediction in ER-negative Breast Cancer显示文摘The estrogen receptor(ER)-negative breast cancer subtype is aggressive with few treatment options available.To identify specific prognostic factors for ER-negative breast cancer,this study included 705,729 and 1034 breast invasive cancer patients from the Surveillance,Epidemiology,and End Results(SEER)and The Cancer Genome Atlas(TCGA)databases,respectively.To identify key differential kinase-substrate node and edge biomarkers between ER-negative and ERpositive breast cancer patients,we adopted a network-based method using correlation coefficients between molecular pairs in the kinase regulatory network.Integrated analysis of the clinical and molecular data revealed the significant prognostic power of kinase-substrate node and edge features for both subtypes of breast cancer.Two promising kinase-substrate edge features,CSNK1A1-NFATC3 and SRC-OCLN,were identified for more accurate prognostic prediction in ERnegative breast cancer patients. | Yidi Sun Chen Li Shichao Pang Qianlan Yao Luonan Chen Yixue Li Rong Zeng | 2020 | Genomics, Proteomics & Bioinformatics2020，18，5： | 1 |
| 17 | Analysis on nuclear heating in the superconducting coils of HT-7U tokamak fusion device显示文摘 | Wu Yican Chen Yixue Huang Qunying | 2003 | Fusion Engineering and Design2003，6668，： | 1 |
| 18 | Monte Carlo based operational and shutdown dose rate calculations of HT-TU tokamak显示文摘 | Chen Yixue Wu Yican Huang Qunying | 2004 | Fusion Engineering and Design2004，70，： | 1 |
| 19 | AP1000 calculation using the reactor physics codes in COSINE project显示文摘 | WANG Changhui CHEN Yixue YU Hui | 2013 | Transactions of the American Nuclear Society2013，108，： | 1 |
| 20 | Adsorption of copper(II) on multiwalled carbon nanotubes in the absence and presence of humic or fulvic acids显示文摘 | Guodong Sheng Jiaxing Li Dadong Shao Jun Hu Changlun Chen Yixue Chen Xiangke Wang | 2010 | Journal of Hazardous Materials2010，，1： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员