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| 1 | TALEN or Cas9-Rapid,Efficient and Specific Choices for Genome Modifications显示文摘Precise modifications of complex genomes at the single nucleotide level have been one of the big goals for scientists working in basic and applied genetics,including biotechnology,drug development,gene therapy and synthetic biology.However,the relevant techniques for making these manipulations in model organisms and human cells have been lagging behind the rapid high throughput studies in the post-genomic era with a bottleneck of low efficiency,time consuming and laborious manipulation,and off-targeting problems.Recent discoveries of TALEs(transcription activator-like effectors) coding system and CRISPR(clusters of regularly interspaced short palindromic repeats) immune system in bacteria have enabled the development of customized TALENs(transcription activator-like effector nucleases) and CRISPR/Cas9 to rapidly edit genomic DNA in a variety of cell types,including human cells,and different model organisms at a very high efficiency and specificity.In this review,we first briefly summarize the development and applications of TALENs and CRISPR/Cas9-mediated genome editing technologies;compare the advantages and constraints of each method;particularly,discuss the expected applications of both techniques in the field of site-specific genome modification and stem cell based gene therapy;finally, propose the future directions and perspectives for readers to make the choices. | Chuanxian Wei Jiyong Liu Zhongsheng Yu Bo Zhang Guanjun Gao Renjie Jiao | 2013 | Journal of Genetics and Genomics2013，40，6： | 52 |
| 2 | Efficient and Specific Modifications of the Drosophila Genome by Means of an Easy TALEN Strategy显示文摘技术开发总是是在生物医学的研究驾驶突破的力量之一。自从托马斯·摩根的时间起,一步一步地, Drosophilists 为操作并且机能上地把开发了强大的基因工具为改进这些技术并且开发新技术的果蝇染色体,而是房间仍然大,今天特别作为生物学家开始系统地学习不同模型有机体的功能的 genomics ,包括人,以一种高产量的方式。这里,我们报导,第一次在果蝇,为借助于改进抄写在很高的效率修改果蝇染色体的一个快速、容易、高度特定的方法像使活跃之物的受动器核酸酶(TALEN ) 策略。我们利用了最近发达的单位集会策略装配设计修改黄基因的二特定的 TALEN (在性染色体上) 并且新奇正染色体的基因。TALEN 的 mRNAs 随后被注入果蝇胚胎。从 31.2% 注射 F0 肥沃的苍蝇,我们检测了包含黄基因的可继承的修正。从到可继承的修正的察觉的特定的 TALEN 的建设的全部过程能在一个月以内被完成。在果蝇的这个调停 TALEN 的染色体修正方法的潜在的应用程序被讨论。 | Jiyong Liu Changqing Li Zhongsheng Yu Peng Huang Honggang Wu Chuanxian Wei Nannan Zhu Yan Shen Yixu Chen Bo Zhang Wu-Min Deng Renjie Jiao | 2012 | Journal of Genetics and Genomics2012，39，5： | 43 |
| 3 | PGC-1α induces apoptosis in human epithelial ovarian cancer cells through a PPARy-dependent pathway显示文摘Peroxisome 激活 proliferator 的受体鲸鱼群妈(PPAR γ) coactivator-1 高山哈(PGC-1 α) coactivates 多重抄写因素并且调整几个代谢过程。Thecurrent 学习在人的上皮的卵巢的癌症房间在 apoptosis 的正式就职调查了 PGC-1 α的角色。在人的卵巢和人的卵巢的上皮的肿瘤之间的 PGC-1 α信使 rna 水平被量的 RT-PCR 检验。更少的 PGC-1 α表示与正常卵巢相比在恶意的肿瘤的表面上皮被发现。在人的上皮的卵巢的癌症房间线 Ho-8910 的 PGC-1 α的 Overexpression 通过 Bcl-2 和 Bax 表示的协调规定导致了房间 apoptosis。Microarray 分析证实 PGC-1 α戏剧性地在 Ho-8910 房间影响了 apoptosis 相关的基因。 Mitochondrial 功能的试金证明 apoptosis 的正式就职通过由细胞色素 c.Furthermore 的版本的终端阶段,导致的 apoptosis 部分是,然而并非完全,由 PPAR γa ntagonist ( GW9662 )堵住了的 PGC-1 α-,并且由 siRNA 的 PPAR γ 表示的抑制也在 Ho-8910 房间禁止了 PGC-1 α- inducedapoptosis 。这些数据建议 PGC-1 α通过 aPPAR γ - 依赖者小径施加了它的效果。我们的调查结果显示 PGC-1 α涉及 apoptotic signaltransduction 小径, PGC-1 α的 down 规定可以是在支持上皮的卵巢的癌症生长和前进的一个关键点。 | Yan Zhang Yi Ba Chang Liu Guoxun Sun Li Ding Songyuan Gao Jihui Hao Zhentao Yu Junfeng Zhang Ke Zen Zhongsheng Tong Yang Xiang Chen-Yu Zhang | 2007 | Cell Research2007，17，4： | 14 |
| 4 | Interpretation of breast cancer screening guideline for Chinese women显示文摘Breast cancer is the most common malignant tumor in Chinese women.Early screening is the best way to improve the rates of early diagnosis and survival of breast cancer patients.The peak onset age for breast cancer in Chinese women is considerably younger than those in European and American women.It is imperative to develop breast cancer screening guideline that is suitable for Chinese women.By summarizing the current evidence on breast cancer screening in Chinese women,and referring to the latest guidelines and consensus on breast cancer screening in Europe,the United States,and East Asia,the China Anti-Cancer Association and National Clinical Research Center for Cancer(Tianjin Medical University Cancer Institute and Hospital)have formulated population-based guideline for breast cancer screening in Chinese women.The guideline provides recommendations on breast cancer screening for Chinese women at average or high risk of breast cancer according to the following three aspects:age of screening,screening methods,and screening interval.This article provides more detailed information to support the recommendations in this guideline and to provide more direction for current breast cancer screening practices in China. | Yubei Huang Zhongsheng Tong Kexin Chen Ying Wang Peifang Liu Lin Gu Juntian Liu Jinpu Yu Fengju Song Wenhua Zhao Yehui Shi Hui Li Huaiyuan Xiao Xishan Hao | 2019 | Cancer Biology & Medicine2019，16，4： | 10 |
| 5 | Benefit of everolimus as a monotherapy for a refractory breast cancer patient bearing multiple genetic mutations in the PI3K/AKT/mTOR signaling pathway显示文摘A postmenopausal patient with a diagnosis of estrogen receptor(ER)(+), progesterone receptor(PR)(+), and human epidermal growth factor receptor-2(HER2)(-) breast cancer was reported. The patient refused surgery and was resistant to conventional chemotherapy regimens. Computed tomography and the circulating tumor cell test indicated that the patient's tumor burden increased rapidly even after several chemotherapy sessions. Multiple genetic aberrances in the phosphatidylinositol3-kinases(PI3 K) signaling pathway were detected via next-generation sequencing(NGS)-based liquid biopsy, including a p. G1007 R missense mutation in exon 21 of PIK3 CA(33.61%), a p.L70 fs frameshift mutation in exon 3 of phosphatase and tension homolog deleted on chromosome ten(PTEN)(49.14%), and a p. D1542 Y missense mutation in exon 32 of mammalian target of rapamycin(m TOR)(1.66%). Therefore, only the m TOR inhibitor everolimus was administered to the patient. Partial remission(PR) was observed after 2 months, and sustained stable disease(SD) was observed after a year and a half. Subsequent sequencing showed that the mutation ratio of PIK3 CA decreased to 4.17%, and that the PTEN and m TOR mutations disappeared, which revealed the significant curative effect of everolimus. We report the first case of successful monotherapy treatment using everolimus in a patient with advanced breast cancer bearing mutations in genes involved in the PI3 K/ARK/m TOR signaling pathway. The success of this case highlights the invaluable clinical contribution of NGS-based liquid biopsy, as it successfully provided an optimal therapeutic target for the patient with advanced breast cancer. | Yehui Shi Wenwen Zhang Yingnan Ye Yanan Cheng Lei Han Pengpeng Liu Weipeng Zhao Zhongsheng Tong Jinpu Yu | 2018 | Cancer Biology & Medicine2018，15，3： | 6 |
| 6 | Drosophila RecQ5 is required for efficient SSA repair and suppression of LOH in vivo显示文摘RecQ5 in mammalian cells has been suggested to suppress inappropriate homologous recombination.However,the specific pathway(s)in which it is involved and the underlining mechanism(s)remain poorly understood.We took advantage of genetic tools in Drosophila to investigate how Drosophila RecQ5(dRecQ5)functions in vivo in homologous recombination-mediated double strand break(DSB)repair.We generated null alleles of dRecQ5 using the targeted recombination technique.The mutant animals are homozygous viable,but with growth retardation during development.The mutants are sensitive to both exogenous DSB-inducing treatment,such as gamma-irradiation,and endogenously induced double strand breaks(DSBs)by I-Sce I endonuclease.In the absence of dRecQ5,single strand annealing(SSA)-mediated DSB repair is compromised with compensatory increases in either inter-homologous gene conversion,or non-homologous end joining(NHEJ)when inter-chromosomal homologous sequence is unavailable.Loss of function of dRecQ5 also leads to genome instability in loss of heterozygosity(LOH)assays.Together,our data demonstrate that dRecQ5 functions in SSA-mediated DSB repair to achieve its full efficiency and in suppression of LOH in Drosophila. | Yixu Chen Wen Dui Zhongsheng Yu Changqing Li Jun Ma Renjie Jiao | 2010 | Protein & Cell2010，1，5： | 5 |
| 7 | Effects of Tidal Channels and Roads on Landscape Dynamic Distribution in the Yellow River Delta, China显示文摘Landscape characters in estuarine regions generally controlled by tidal regimes and human activities like road construction.In this work,tidal channels and road construction in the Yellow River Delta(YRD)were extracted by visual interpretation methods so as to decipher impacts of tidal channel development and road construction on landscape patch change during 1989–2016.Spatial distribution history of three wetlands,which covered by Phragmites australis(freshwater marsh,FM),Suaeda salsa(salt marsh,SM),and mudflats(MD)were also established.Results indicated that tidal channel,number,frequency,and fractal dimension were all the maximum in 2003,and the minimum in 1998,respectively.Road length,number,and density showed increasing trend during 1989–2016.MD were the predominant landscape type,followed by FM and SM during 1989-2016.Principal component analysis implied two extracted factors,F1 and F2,which could represent 91.93% of the total variations.F1 mainly proxied tidal channel development,while F2 represented road construction.A multiple linear regression analysis showed positive effects of both F1 and F2 on FM patch numbers and negative impacts on SM patch areaes with R^2 values of 0.416 and 0.599,respectively.Tidal channels were negatively related to MD patch numbers,while roads were positively related to that.In any case,road construction showed larger impacts on landscape type shifting than that of tidal channel development in the YRD. | YU Xiaojuan ZHANG Zhongsheng XUE Zhenshan WU Haitao ZHANG Hongri | 2020 | Chinese Geographical Science2020，30，1： | 2 |
| 8 | Autofluorescence imaging endoscopy for predicting acid reflux in patients with gastroesophageal reflux disease显示文摘 | Weifeng Wang Noriya Uedo Yunsheng Yang Lihua Peng Diane Bai Zhongsheng Lu Kaichun Fan Juan Wang Xiaoxiao Wang Yunlong Zhao Zhiping Yu | 2014 | J Gastroenterol Hepatol2014，，7： | 1 |
| 9 | Predicting corporate financial distress based on integration of support vector machines and logistic regression显示文摘 | Hua Zhongsheng Wang Yu Xu Xiaoyan | 2007 | Expert Systems With Applications2007，33，2： | 1 |
| 10 | Analysis of mechanical behaviors of big pipe roof for shallow buried large-span tunnel显示文摘A series of researches on mechanical behaviors of big pipe roof for shallow large-span loess tunnel were carried out based on the Wenxiang tunnel in Zhengzhou—Xi'an Special Passenger Railway. The longitudinal deformations of the pipe roofs were monitored and the mechanical behaviors of the pipe roofs were analyzed at the test section. A new double-parameter elastic foundation beam model for pipe roof in shallow tunnels was put forward in Wenxiang tunnel. The measured values and the calculation results agreed well with each other,revealing the force-deformation law of big pipe roof in loess tunnel:At about 15 m in front of the excavating face,the pipe roof starts to bear the load;at about 15 m behind the excavating face,the force of the pipe roof tends to be stabilized;the longitudinal deformation of the whole pipe roofs is groove-shaped distribution,and the largest force of pipe roofs is at the excavating face. Simultaneously,the results also indicate that mechanical behaviors of pipe roof closely relate to the location of the excavation face,the footage of the tunnelling cycle and the mechanics parameters of pipe roof and rock. The conclusions can be reference for the design parameter optimization and the construction scheme selection of pipe roofs,and have been verified by the result of numerical analysis software FLAC3Dand deformation monitoring. | Li Jian Tan Zhongsheng Yu Yu Guo Xiaohong | 2013 | Engineering Sciences2013，11，6： | 0 |
| 11 | Development of urban underground space in coastal cities in China:A review显示文摘The development of urban underground space is a critical way to alleviate the lack of urban resources,and improve environmental conditions as well as residents'quality of life,which has important practical significance.As the most densely populated and economically developed areas,coastal cities face many challenges in underground space development owing to their unique environmental geological conditions.This study examined the current situation of underground space development,academic research,technology application,evaluation methods,planning,and legislation in coastal cities in China.On this basis,it analyzed the future development trends,and presented reasonable suggestions for the critical scientific and technical problems faced by the development of underground space in coastal cities.The key suggestions are as follows:it is necessary to define and clarify the critical geological issues,distributions,and hazards that affect underground space development and major engineering construction in coastal cities;the land-sea integrated detection technology for sea crossing bridges and submarine tunnels needs to be studied;in evaluating the suitability of underground space in coastal cities,the land and sea area evaluation factors should be integrated;and the evaluation system of landsea integration needs to be established. | Peng Yu Honghua Liu Zhongsheng Wang Jiani Fu Hui Zhang Jia Wang Qi Yang | 2023 | Deep Underground Science and Engineering2023，2，2： | 0 |
| 12 | High special surface area and“warm light”responsive ZnO:Synthesis mechanism,application and optimization显示文摘In this study,a new series of zinc oxide(ZnO)with high specific surface area and narrow energy band gap are prepared using a facile microwave-induced method.The corresponding formation mechanism is also discussed for the first time.Due to the introduction of C,these ZnO can be excited by long wave temperature light without harmful short wave radiation,and play an efficient photocatalytic activity.This valuable property fundamentally improves the biological safety of its photocatalytic application.Herein,taking teeth whitening as an example,the photocatalytic performance of ZnO is evaluated.The“pure”yellow light-emitting diode(PYLED)with high biological safety is used as the excitation source.It is found that this method could effectively remove pigment on the tooth surface through physical adsorption.In addition,these ZnO could generate active oxygen to degrade the pigment on the tooth surface under the irradiation of yellow light.Some further optimization of these“warm light”responsive ZnO is also discussed in this systematical study,which could open up new opportunities in biomedical field. | Xinxin Miao Fen Yu Kuan Liu Zhongsheng Lv Jianjian Deng Tianlong Wu Xinyan Cheng Wei Zhang Xigao Cheng Xiaolei Wang | 2022 | Bioactive Materials2022，7，1： | 0 |
| 13 | Machine learning modeling identifies hypertrophic cardiomyopathy subtypes with genetic signature显示文摘Previous studies have revealed that patients with hypertrophic cardiomyopathy(HCM)exhibit differences in symptom severity and prognosis,indicating potential HCM subtypes among these patients.Here,793 patients with HCM were recruited at an average follow-up of 32.78±27.58 months to identify potential HCM subtypes by performing consensus clustering on the basis of their echocardiography features.Furthermore,we proposed a systematic method for illustrating the relationship between the phenotype and genotype of each HCM subtype by using machine learning modeling and interactome network detection techniques based on whole-exome sequencing data.Another independent cohort that consisted of 414 patients with HCM was recruited to replicate the findings.Consequently,two subtypes characterized by different clinical outcomes were identified in HCM.Patients with subtype 2 presented asymmetric septal hypertrophy associated with a stable course,while those with subtype 1 displayed left ventricular systolic dysfunction and aggressive progression.Machine learning modeling based on personal whole-exome data identified 46 genes with mutation burden that could accurately predict subtype propensities.Furthermore,the patients in another cohort predicted as subtype 1 by the 46-gene model presented increased left ventricular end-diastolic diameter and reduced left ventricular ejection fraction.By employing echocardiography and genetic screening for the 46 genes,HCM can be classified into two subtypes with distinct clinical outcomes. | Jiaqi Dai Tao Wang Ke Xu Yang Sun Zongzhe Li Peng Chen Hong Wang Dongyang Wu Yanghui Chen Lei Xiao Hao Liu Haoran Wei Rui Li Liyuan Peng Ting Yu Yan Wang Zhongsheng Sun Dao Wen Wang | 2023 | Frontiers of Medicine2023，17，4： | 0 |
| 14 | Genome-wide DNA methylation landscape of four Chinese populations and epigenetic variation linked to Tibetan high-altitude adaptation显示文摘DNA methylation(DNAm)is one of the major epigenetic mechanisms in humans and is important in diverse cellular processes.The variation of DNAm in the human population is related to both genetic and environmental factors.However,the DNAm profiles have not been investigated in the Chinese population of diverse ethnicities.Here,we performed double-strand bisulfite sequencing(DSBS)for 32 Chinese individuals representing four major ethnic groups including Han Chinese,Tibetan,Zhuang,and Mongolian.We identified a total of 604,649 SNPs and quantified DNAm at more than 14 million Cp Gs in the population.We found global DNAm-based epigenetic structure is different from the genetic structure of the population,and ethnic difference only partially explains the variation of DNAm.Surprisingly,non-ethnic-specific DNAm variations showed stronger correlation with the global genetic divergence than these ethnic-specific DNAm.Differentially methylated regions(DMRs)among these ethnic groups were found around genes in diverse biological processes.Especially,these DMR-genes between Tibetan and nonTibetans were enriched around high-altitude genes including EPAS1 and EGLN1,suggesting DNAm alteration plays an important role in high-altitude adaptation.Our results provide the first batch of epigenetic maps for Chinese populations and the first evidence of the association of epigenetic changes with Tibetans'high-altitude adaptation. | Zeshan Lin Yan Lu Guoliang Yu Huajing Teng Bao Wang Yajun Yang Qinglan Li Zhongsheng Sun Shuhua Xu Wen Wang Peng Tian | 2023 | Science China(Life Sciences)2023，66，10： | 0 |
| 15 | CACA Guidelines for Holistic Integrative Management of Breast Cancer显示文摘Purpose:Breast cancer is now the most common malignant tumor worldwide.About one-fourth of female cancer patients all over the world sufer from breast cancer.And about one in six female cancer deaths worldwide is caused by breast cancer.In terms of absolute numbers of cases and deaths,China ranks frst in the world.The CACA Guidelines for Holistic Integrative Management of Breast Cancer were edited to help improve the diagnosis and comprehensive treatment in China.Methods:The Grading of Recommendations Assessment,Development and Evaluation(GRADE)was used to classify evidence and consensus.Results:The CACA Guidelines for Holistic Integrative Management of Breast Cancer include the epidemiology of breast cancer,breast cancer screening,breast cancer diagnosis,early breast cancer treatment,advanced breast cancer treatment,follow-up,rehabilitation,and traditional Chinese medicine treatment of breast cancer patients.Conclusion:We to standardize the diagnosis and treatment of breast cancer in China through the formulation of the CACA Guidelines. | Jiong Wu Daiming Fan Zhimin Shao Binghe Xu Guosheng Ren Zefei Jiang Yongsheng Wang Feng Jin Jin Zhang Qingyuan Zhang Fei Ma Jinli Ma Zhonghua Wang Shusen Wang Xiaojia Wang Shu Wang Haibo Wang Tao Wang Xiang Wang Jing Wang Jia Wang Biyun Wang Li Fu Hongyuan Li Yehui Shi Lu Gan Yunjiang Liu Jian Liu Zhenzhen Liu Qiang Liu Qiang Sun Wenwu Cheng Keda Yu Zhongsheng Tong Xinhong Wu Chuangui Song Jianguo Zhang Jian Zhang Junjie Li Bin Li Man Li Huiping Li Wentao Yang Hongjian Yang Benlong Yang Hong Bu Juping Shen Zhenzhou Shen Yiding Chen Ceshi Chen Da Pang Zhimin Fan Ying Zheng Xiaoli Yu Guangyu Liu Xichun Hu Yiqun Ling Jinhai Tang Yongmei Yin Cuizhi Geng Peng Yuan Yajia Gu Cai Chang Xuchen Cao Yuan Sheng Yuanxi Huang Jian Huang Weijun Peng Xiaohua Zeng Yuntao Xie Ning Liao Committee of Breast Cancer Society,Chinese Anti-Cancer Association | 2022 | Holistic Integrative Oncology2022，1，1： | 0 |
| 16 | Acupoint Catgut Embedding attenuates immune-inflammatory response in hippocampus of vascular dementia rats by inhibiting TLR4/My D88 signaling pathway显示文摘Acupoint Catgut Embedding(ACE)has been proposed as a beneficial treatment for vascular dementia(VaD),but the underlying mechanisms are still unclear.The present study investigated whether ACE alleviates cognitive impairment by suppressing TLR4/MyD88 signaling pathway,which triggers immune-inflammatory responses in the hippocampus.V aD was established by 4-VO in male SD rats.Eight days after the operation,rats were treated with ACE once a week for 3 consecutive weeks.'T ransmission electron microscopy was used to observe the ultrastructure of nerve cells in the hippocampal area before and after ACE treatment. | Bu Yu Li Wenshuang Lin Ji Wei Yuwvei Sun Qiuying Zhu Shijie Tang Zhongsheng | 2021 | 解剖学杂志2021，44，S01： | 0 |
| 17 | Morphology modulation of artificial muscles by thermodynamic-twist coupling显示文摘Human muscles can grow and change their length with body development;therefore,artificial muscles that modulate their morphology according to changing needs are needed.In this paper,we report a strategy to transform an artificial muscle into a new muscle with a different morphology by thermodynamic-twist coupling,and illustrate its structural evolution during actuation.The muscle length can be continuously modulated over a large temperature range,and actuation occurs by continuously changing the temperature.This strategy is applicable to different actuation modes,including tensile elongation,tensile contraction and torsional rotation.This is realized by twist insertion into a fibre to produce torsional stress.Fibre anne aling causes partial thermodynamic relaxation of the spiral molecular chains,which serves as internal tethering and inhibits fibre twist release,thus producing a self-supporting artificial muscle that actuates under heating.At a sufficiently high temperature,further relaxation of the spiral molecular chains occurs,resulting in a new mus cle with a different length.A structural study provides an understanding of the thermo dynamic-twist coupling.This work provides a new design strategy for intelligent materials. | Xiaoyu Hu Jiatian Li Sitong Li Guanghao Zhang Run Wang Zhongsheng Liu Mengmeng Chen Wenqian He Kaiqing Yu Wenzhong Zhai Weiqiang Zhao Abdul Qadeer Khan Shaoli Fang Ray HBaughman Xiang Zhou Zunfeng Liu | 2023 | National Science Review2023，10，1： | 0 |
| 18 | Differential regulation of JAK1 expression by ETS1 associated with predisposition to primary biliary cholangitis显示文摘Primary biliary cholangitis(PBC)is an autoimmune liver disease characterized by the destruction of intrahepatic small bile ducts and progressive cholestasis,eventually leading to liver cirrhosis and hepatic failure without appropriate treatment(Terziroli Beretta-Piccoli et al.,2019). | Peng Jiang Chan Wang Mingming Zhang Ye Tian Weifeng Zhao Junyi Xin Yexi Huang Zhibin Zhao Wenjuan Sun Jie Long Ruqi Tang Fang Qiu Xingjuan Shi Yi Zhao Li Zhu Na Dai Lei Liu Xudong Wu Jinshan Nie Bo Jiang Youlin Shao Yueqiu Gao Jianjiang Yu Zhigang Hu Zhidong Zang Yuhua Gong Yaping Dai Lan Wang Ningling Ding Ping Xu Sufang Chen Lu Wang Jing Xu Luyao Zhang Junyan Hong Ruonan Qian Hu Li Xuan Jiang Congwei Chen Wenyan Tian Jian Wu Yuzhang Jiang Chongxu Han Kui Zhang Hong Qiu Li Li Hong Fan Liming Chen Jianqiong Zhang Zhongsheng Sun Xiao Han Zhenhua Dai Erguang Li M.Eric Gershwin Zhexiong Lian Xiong Ma Michael F.Seldin Weichang Chen Meilin Wang Xiangdong Liu | 2023 | Journal of Genetics and Genomics2023，50，10： | 0 |
| 19 | Advances in Drosophila gene targeting and related techniques显示文摘Functional biological research has benefited tremendously by analyses of the phenotypes of mutant organisms which can be generated through targeted mutation of genes.In Drosophila,compared with random mutagenesis methods gene targeting has gained its popularity because it can introduce any desired mutation into a gene of interest.However,applications of gene targeting have been limited because the targeting efficiency varies with different genes,and the time and labor of targeting procedure are intensive.Nevertheless,improvement of gene targeting and development of its variant technologies have received much attention of scientists.Here we review recent progress that has been made in expanding the applications of gene targeting,which include the ϕC31 integration system and zinc-finger nucleases induced gene targeting,and new strategies that generate more efficient and reliable gene targeting. | Zhongsheng YU Renjie JIAO | 2010 | Frontiers in Biology2010，5，3： | 0 |
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