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| 1 | Molecular distribution and seasonal variation of hydrocarbons in PM_(2.5) from Beijing during 2006显示文摘正常(n) 链烷和多不的芳香的烃(哼) 在 2006 从北京在 PM2.5 被收集并且分析了使用一种热 desorption-GC/MS 技术。n 链烷的年度平均集中并且哼 282 是 ????????????? ? ㈠ ?? 朠洯″ ???? ?? | Yushan Li Junji Cao Jianjun Li Jiamao Zhou Hongmei Xu Renjian Zhang Zhiyun Ouyang | 2013 | Particuology2013，11，1： | 5 |
| 2 | Interval mapping for red/green skin color in Asian pears using a modified QTL-seq method显示文摘Pears with red skin are attractive to consumers and provide additional health benefits.Identification of the gene(s)responsible for skin coloration can benefit cultivar selection and breeding.The use of QTL-seq,a bulked segregant analysis method,can be problematic when heterozygous parents are involved.The present study modified the QTL-seq method by introducing a|Δ(SNP-index)|parameter to improve the accuracy of mapping the red skin trait in a group of highly heterozygous Asian pears.The analyses were based on mixed DNA pools composed of 28 red-skinned and 27 green-skinned pear lines derived from a cross between the‘Mantianhong’and‘Hongxiangsu’red-skinned cultivars.The‘Dangshansuli’cultivar genome was used as reference for sequence alignment.An average single-nucleotide polymorphism(SNP)index was calculated using a sliding window approach(200-kb windows,20-kb increments).Nine scaffolds within the candidate QTL interval were in the fifth linkage group from 111.9 to 177.1 cM.There was a significant linkage between the insertions/deletions and simple sequence repeat markers designed from the candidate intervals and the red/green skin(R/G)locus,which was in a 582.5-kb candidate interval that contained 81 predicted protein-coding gene models and was composed of two subintervals at the bottom of the fifth chromosome.The ZFRI 130-16,In2130-12 and In2130-16 markers located near the R/G locus could potentially be used to identify the red skin trait in Asian pear populations.This study provides new insights into the genetics controlling the red skin phenotype in this fruit. | Huabai Xue Ting Shi Fangfang Wang Huangkai Zhou Jian Yang Long Wang Suke Wang Yanli Su Zhen Zhang Yushan Qiao Xiugen Li | 2017 | Horticulture Research2017，4，1： | 5 |
| 3 | Extraction and back-extraction behaviors of 14 rare earth elements from sulfuric acid medium by TODGA显示文摘The unique physical and chemical properties of rare earth elements lay the foundation for their extensive application. N,N,N',N' Tetra-octyl-3-oxopentanediamide(TODGA) is excellent in its ability of extracting rare earth elements and it is favored for green initiative. In this paper, the extraction and back-extraction of14 rare earth elements by TODGA were studied. Experiments show that in conditions of 6 mol/L sulfuric acid, the extraction temperature of 25 ℃,the phase ratio of 1:1 and 0.04 mol/LTODGA(aviation kerosene as the diluent), the extraction rates of 14 rare earth elements including lanthanum, cerium, praseodymium,neodymium, europium, gadolinium, terbium, dysprosium, holmium, erbium, thulium, ytterbium, lutetium, and yttrium were 99.00%-99.73%. Mixed with hydrochloric acid and nitric acid(HCl 3.5 mol/L, HNO_30.5 mol/L), the recoveries of the 14 rare earth elements are 91.52%-99.91% when the extraction temperature is 25 ℃ and the ratio is 1:1. The following application is based on the optimum conditions above with practical samples(from the roasting production line of China North Rare Earth High-tech Company Limited) for extraction and back-extraction experiments. Experiments show that TODGA has excellent enrichment effect on 14 rare earth elements, the extraction rates are 91.36%-99.80%, the back-extraction rates are 87.29%-99.64% and the total recoveries are 81.19%-99.44%. | Huiting Yuan Weixiang Hong Yushan Zhou Baisong Pu Aijun Gong Tao Xu Qishan Yang Fukai Li Lina Qiu Weiwei Zhang Yuning Liu | 2018 | Journal of Rare Earths2018，36，6： | 4 |
| 4 | Regulation of mATG9 trafficking by Src- and ULK1mediated phosphorylation in basal and starvation-induced autophagy显示文摘Autophagy 要求多样的膜来源并且包含 mATG9 的膜 trafficking,在 ATG 家庭的唯一的膜蛋白质。然而,为 autophagy 开始的 mATG9 trafficking 的分子的规定仍然保持不清楚。这里,我们识别了在 mATG9 的 cytosolic N 终点以内排序信号的二保存经典适配器蛋白质,它调停从经由和 AP1/2 建筑群的相互作用的血浆膜和 trans-Golgi 网络(TGN ) 的 mATG9 的 trafficking。在 Tyr8 到的 Src phosphorylates mATG9 处于不着重的条件维持它的 endocytic 和组成的 trafficking。响应饥饿,在由 Src 的 Tyr8 并且在由 ULK1 的 Ser14 的 mATG9 的 phosphorylation 机能上地合作支持在 mATG9 和 AP1/2 建筑群之间的相互作用,为 autophagy 从血浆膜和 juxta 原子的区域导致 mATG9 的再分配到外部水池开始。我们的调查结果揭开 mATG9 trafficking 的新奇机制并且建议基础、导致压力的 autophagy 的协作。 | Changqian Zhou Kaili Ma Ruize Gao Chenglong Mu Linbo Chen Qiangqiang Liu Qian Luo Du Feng Yushan Zhu Quan Chen | 2017 | Cell Research2017，27，2： | 2 |
| 5 | Fabrication of Sulfated Silk Fibroin‑Based Blend Nanofbrous Membranes for Lysozyme Adsorption显示文摘In this work,the silk fbroin/cellulose blend nanofbrous membranes modifed with sodium-3-sulfobenzoate(S-SCBNM)were fabricated by electrospinning technique for lysozyme adsorption.The morphology and structure of membranes was observed.The efect of sulfate contents,pH values and concentration of lysozyme on adsorption performance were studied,respectively.The reuse capacity was evaluated.The results showed that the resultant S-SCBNM exhibited integrated properties of ultrathin fber diameter(148 nm),fast adsorption equilibrium,excellent adsorption performance(636 mg g−1)and good reversibility.We envision that this new class of green and natural blend membranes is particularly promising for the development of proteins separation. | Ying Zhou Jiaxue Wu Yanyi Li Wei Zhang Yushan Zou Lian Duan Xiao Yang Bo Xiao Shixiong Yi | 2022 | Advanced Fiber Materials2022，4，1： | 1 |
| 6 | Haemophilus parasuis infection activates the NF-κB pathway in PK-15 cells through IκB degradation显示文摘 | Yushan Chen Hui Jin Ping Chen Zili Li Xianrong Meng Mei Liu Shaowen Li Deshi Shi Yuncai Xiao Xiliang Wang Zutao Zhou Dingren Bi Rui Zhou | 2012 | Veterinary Microbiology (-)2012，，1： | 1 |
| 7 | Preclinical characterization and comparison between CD3/CD19 bispecific and novel CD3/CD19/CD20 trispecific antibodies against B-cell acute lymphoblastic leukemia:targeted immunotherapy for acute lymphoblastic leukemia显示文摘The CD19-targeting bispecific T-cell engager blinatumomab has shown remarkable efficacy in patients with relapsed/refractory B-cell precursor acute lymphoblastic leukemia.However,several studies showed that blinatumomab has a short plasma half-life due to its low molecular weight,and thus its clinical use is limited.Furthermore,multiple trials have shown that approximately 30%of blinatumomab-relapsed cases are characterized by CD19 negative leukemic cells.Here,we design and characterize two novel antibodies,A-319 and A-2019.Blinatumomab and A-319 are CD3/CD19 bispecific antibodies with different molecular sizes and structures,and A-2019 is a novel CD3/CD19/CD20 trispecific antibody with an additional anti-CD20 function.Our in vitro,ex vivo,and in vivo experiments demonstrated that A-319 and A-2019 are potent antitumor agents and capable of recruiting CD3 positive T cells,enhancing T-cell function,mediating B-cell depletion,and eventually inhibiting tumor growth in Raji xenograft models.The two molecules are complementary in terms of efficacy and specificity profile.The activity of A-319 demonstrated superior to that of A-2019,whereas A-2019 has an additional capability to target CD20 in cells missing CD19,suggesting its potential function against CD19 weak or negative CD20 positive leukemic cells. | Sisi Wang Lijun Peng Wenqian Xu Yuebo Zhou Ziyan Zhu Yushan Kong Stewart Leung Jin Wang Xiaoqiang Yan Jian-Qing Mi | 2022 | Frontiers of Medicine2022，16，1： | 1 |
| 8 | Dual-responsive nanoparticles with transformable shape and reversible charge for amplified chemo-photodynamic therapy of breast cancer显示文摘Herein, we designed a dual-response shape transformation and charge reversal strategy with chemo-photodynamic therapy to improve the blood circulation time, tumor penetration and retention,which finally enhanced the anti-tumor effect. In the system, hydrophobic photosensitizer chlorin e6(Ce6), hydrophilic chemotherapeutic drug berberrubine(BBR) and matrix metalloproteinase-2(MMP-2) response peptide(PLGVRKLVFF) were coupled by linkers to form a linear triblock molecule BBR-PLGVRKLVFF-Ce6(BPC), which can self-assemble into nanoparticles. Then, positively charged BPC and polyethylene glycol-histidine(PEG-His) were mixed to form PEG-His@BPC with negative surface charge and long blood circulation time. Due to the acidic tumor microenvironment, the PEG shell was detached from PEG-His@BPC attributing to protonation of the histidine, which achieved charge reversal, size reduction and enhanced tumor penetration. At the same time, enzyme cutting site was exposed, and the spherical nanoparticles could transform into nanofibers following the enzymolysis by MMP-2, while BBR was released to kill tumors by inducing apoptosis. Compared with original nanoparticles, the nanofibers with photosensitizer Ce6 retained within tumor site for a longer time. Collectively,we provided a good example to fully use the intrinsic properties of different drugs and linkers to construct tumor microenvironment-responsive charge reversal and shape transformable nanoparticles with synergistic antitumor effect. | Wenfeng Jia Rui Liu Yushan Wang Chuan Hu Wenqi Yu Yang Zhou Ling Wang Mengjiao Zhang Huile Gao Xiang Gao | 2022 | Acta Pharmaceutica Sinica B2022，12，8： | 1 |
| 9 | Pepper mild mottle virus coat protein interacts with pepper chloroplast outer envelope membrane protein OMP24 to inhibit antiviral immunity in plants显示文摘Pepper mild mottle virus(PMMoV)is a devastating viral pathogen of pepper(Capsicum annuum)but it is unclear whether and how peppers protect against PMMoV infection.The expression of the chloroplast outer membrane protein 24(OMP24)of C.annuum was upregulated under PMMoV infection and it interacted with PMMoV coat protein(CP).Silencing of OMP24 in either C.annuum or Nicotiana benthamiana facilitated PMMoV infection,whereas overexpression of N.benthamiana OMP24 in transgenic plants inhibited PMMoV infection.Both C.annuum OMP24(CaOMP24)and N.benthamiana OMP24(NbOMP24)localized to the chloroplast and have a moderately hydrophobic transmembrane domain that is necessary for their localization.Overexpression of CaOMP24 induced stromules,perinuclear chloroplast clustering,and accumulation of reactive oxygen species(ROS),the typical defense responses of chloroplasts transferring the retrograde signaling to the nucleus to regulate resistance genes.The expression of PR1 and PR2 was also upregulated significantly in plants overexpressing OMP24.Self-interaction of OMP24 was demonstrated and was required for OMP24-mediated plant defense.Interaction with PMMoV CP interfered with the self-interaction of OMP24 and impaired OMP24-induced stromules,perinuclear chloroplast clustering and ROS accumulation.The results demonstrate the defense function of OMP24 in pepper during viral infection and suggest a possible mechanism by which PMMoV CP modulates the plant defense to facilitate viral infection. | Kelei Han Hongying Zheng Dankan Yan Huijie Zhou Zhaoxing Jia Yushan Zhai Jian Wu Yuwen Lu Guanwei Wu Shaofei Rao Jianping Chen Jiejun Peng Rende Qi Fei Yan | 2023 | Horticulture Research2023，10，5： | 1 |
| 10 | Stress corrosion cracking of EV31A in 0.1 M Na_(2)SO_(4) saturated with Mg(OH)_(2)显示文摘This paper studied the stress corrosion cracking(SCC)of EV31A in 0.1 M Na 2 SO 4 saturated with Mg(OH)2 using linearly increasing stress tests,compared with pure Mg and WE43B.All three materials were susceptible to SCC.SCC susceptibility increased with decreasing applied stress rate.The threshold stress was 0.3×(yield stress)for pure Mg,0.6×(yield stress)for EV31A,and 0.8×(yield stress)for WE43B.The SCC velocities at an applied stress rate of 7.3×10^(-4)MPa s^(-1)were 7.2×10^(-8)m s^(−1)for pure Mg,5.6×10^(-9)m s^(-1)for WE43B,and 1.5×10^(-9)m s^(-1)for EV31A. | Yifeng Li Yushan Zhou Zhiming Shi Jeffrey Venezuela Akif Soltan Andrej Atrens | 2018 | Journal of Magnesium and Alloys2018，6，4： | 0 |
| 11 | Fast,simple,efficient Agrobacterium rhizogenes-mediated transformation system to non-heading Chinese cabbage with transgenic roots显示文摘Non-heading Chinese cabbage, a variety of Brassica campestris, is an important vegetable crop in the Yangtze River Basin of China. However,the immaturity of its stable transformation system and its low transformation efficiency limit gene function research on non-heading Chinese cabbage. Agrobacterium rhizogenes-mediated(ARM) transgenic technology is a rapid and effective transformation method that has not yet been established for non-heading Chinese cabbage plants. Here, we optimized conventional ARM approaches(one-step and two-step transformation methods) suitable for living non-heading Chinese cabbage plants in nonsterile environments. Transgenic roots in composite non-heading Chinese cabbage plants were identified using phenotypic detection, fluorescence observation, and PCR analysis. The transformation efficiency of a two-step method on four five-day-old non-heading Chinese cabbage seedlings(Suzhouqing, Huangmeigui, Wuyueman, and Sijiu Caixin) was 43.33%-51.09%, whereas using the stout hypocotyl resulted in a transformation efficiency of 54.88% for the 30-day-old Sijiu Caixin.The one-step method outperformed the two-step method;the transformation efficiency of different varieties was above 60%, and both methods can be used to obtain transgenic roots for functional studies within one month. Finally, optimized ARM transformation methods can easily,quickly, and effectively produce composite non-heading Chinese cabbage plants with transgenic roots, providing a reliable foundation for gene function research and non-heading Chinese cabbage genetic improvement breeding. | Huiyu Wang Yushan Zheng Qian Zhou Ying Li Tongkun Liu Xilin Hou | 2024 | Horticultural Plant Journal2024，10，2： | 0 |
| 12 | Microbial Limit Test of Zhideke Granules显示文摘[Objectives]To test the microbial limit of Zhideke granules.[Methods]According to the relevant regulations on the testing of granules in Chinese Pharmacopoeia in 2015,the applicability of the methods for counting the total number of aerobic bacteria,molds and yeasts and objectionable organisms in Zhideke granules was studied.[Results]The total number of aerobic bacteria in Zhideke granules was determined by dilution plate method(1∶20,1 mL per dish),the total number of molds and yeasts was determined by conventional plate method(1∶10,1 mL per dish),and E.coli was detected by conventional method of TTB(1∶10 test solution,10-100 mL TSB).[Conclusions]The method was suitable for the microbial limit test of Zhideke granules. | Yunjuan PANG Yushan ZHOU Jie LIANG Hui XU Jinyu WEI Dongfang HUANG Zhengyi SUN | 2021 | Medicinal Plant2021，12，3： | 0 |
| 13 | Parallel Transitional Rules of Items in Parsing显示文摘 | Yushan Sun Lei Zhou Yuqiang Sun Zhenghua Ma | 2005 | 通讯和计算机(中英文版)2005，2，9： | 0 |
| 14 | Double Beams to Measure Three-dimensional Rigid Body Displacements | ZHOU Jian,CHEN Wenyi,ZHAO Hong TIAN Feng,TAN Yushan (Research Institute for Laser and Infrared Applications,Xi’an Jiaotong University,Xi’an 710049,CHN) | 1997 | Semiconductor Photonics and Technology1997，3，2： | 0 |
| 15 | Sedimentary filling characteristics and controlling factors of lacustrine microbial carbonates sequence in the Santos Basin,Brazil显示文摘Based on comprehensive analysis of seismic,logging,core,thin section data,and stable isotopic compositions of carbon and oxygen,the sedimentary filling characteristics of the Lower Cretaceous Barra Velha Formation sequence in H oil field,Santos Basin,are studied,and the high-frequency sequence stratigraphic framework is established,and the spatial distribution of reef-shoal bodies are predicted and the controlling factors are discussed.During the depositional period of the Barra Velha Formation,the study area is a slope-isolated platform-slope sedimentary pattern from southwest to northeast and the change of climate background from rift to depression periods has resulted in the variation of sedimentary characteristics from the lower third-order sequence SQ1(BVE 300 Member)of low-energy deep water to the upper third-order sequence SQ2(BVE 200 and 100 members)of high-energy shallow water in the Barra Velha Formation.The activities of extensional faults and strike-slip faults in rift period and the sedimentary differentiation from platform margin to intra-platform in depression period made the sedimentary paleogeomorphology in these two periods show features of“three ridges and two depressions”.The reef-shoal bodies mainly developed in the SQ2-LHST period,with vertical development positions restricted by the periodic oscillation of the lake level,and developed on the top of each high-frequency sequence stratigraphic unit in SQ2-LHST in the platform.The strike-slip fault activity controlled the distribution of the reef-shoal bodies on the plane by changing the sedimentary paleogeomorphology.The positive flower-shaped strike-slip faults made the formation of local highlands at the margins of and inside the shallow water platforms and which became high-energy sedimentary zones,creating conditions for the development of reef-shoal bodies. | HE Sai LI Guorong WU Changrong LIU Shugen ZHANG Zhongmin SU Yushan ZHU Yixuan HE Zhao REN Jie WANG Yuchen ZHOU Wei WANG Yajie | 2022 | Petroleum Exploration and Development2022，49，4： | 0 |
| 16 | Sperm cryopreservation in the human sperm bank:11-year results of the Chinese mainland sperm banking network显示文摘To the Editor:Montegazza in 1866 was the first to suggest banks for frozen human semen.[1]In 1981,China's first human sperm bank was successfully established at the CITIC Xiangya Hospital.During the previous four decades,from 1981 to 2020,there were 27 human sperm banks in Chinese mainland.Human sperm banks provide cryopreservation of spermatozoa services for males needing to preserve fertility for an extended period in the Chinese mainland. | Xiao Liu Qiling Wang Wenbing Zhu Zhou Zhang Wenhao Tang Huiqiang Sheng Jigao Yang Yushan Li Xiaowei Liang Tianqing Meng Zhiqiang Wang Faxi Lin Hao Dong Xiaojin He Xianglong Jiang Shanjun Dai Aiping Zhang Chunying Song Zuowen Liang Feng Zhang Xiaojun Wang Peiyu Liang Guihua Gong Xiaohong Huai Fuping Xinzong Zhang | 2022 | Chinese Medical Journal2022，135，22： | 0 |
| 17 | Multiomics analysis reveals metabolic subtypes and identifies diacylglycerol kinase α (DGKA) as a potential therapeutic target for intrahepatic cholangiocarcinoma显示文摘Background:Intrahepatic cholangiocarcinoma(iCCA)is a highly heteroge-neous and lethal hepatobiliary tumor with few therapeutic strategies.The metabolic reprogramming of tumor cells plays an essential role in the develop-ment of tumors,while the metabolic molecular classification of iCCA is largely unknown.Here,we performed an integrated multiomics analysis and metabolic classification to depict differences in metabolic characteristics of iCCA patients,hoping to provide a novel perspective to understand and treat iCCA.Methods:We performed integrated multiomics analysis in 116 iCCA samples,including whole-exome sequencing,bulk RNA-sequencing and proteome anal-ysis.Based on the non-negative matrix factorization method and the protein abundance of metabolic genes in human genome-scale metabolic models,the metabolic subtype of iCCA was determined.Survival and prognostic gene analy-ses were used to compare overall survival(OS)differences between metabolic subtypes.Cell proliferation analysis,5-ethynyl-2’-deoxyuridine(EdU)assay,colony formation assay,RNA-sequencing and Western blotting were performed to investigate the molecular mechanisms of diacylglycerol kinaseα(DGKA)in iCCA cells.Results:Three metabolic subtypes(S1-S3)with subtype-specific biomarkers of iCCA were identified.These metabolic subtypes presented with distinct prog-noses,metabolic features,immune microenvironments,and genetic alterations.The S2 subtype with the worst survival showed the activation of some special metabolic processes,immune-suppressed microenvironment and Kirsten ratsar-coma viral oncogene homolog(KRAS)/AT-rich interactive domain 1A(ARID1A)mutations.Among the S2 subtype-specific upregulated proteins,DGKA was further identified as a potential drug target for iCCA,which promoted cell proliferation by enhancing phosphatidic acid(PA)metabolism and activating mitogen-activated protein kinase(MAPK)signaling.Conclusion:Viamultiomics analyses,we identified three metabolic subtypes of iCCA,revealing that the S2 subtype exhibited the poorest survival outcomes.We further identified DGKA as a potential target for the S2 subtype. | Weiren Liu Huqiang Wang Qianfu Zhao Chenyang Tao Weifeng Qu Yushan Hou Run Huang Zimei Sun Guiqi Zhu Xifei Jiang Yuan Fang Jun Gao Xiaoling Wu Zhixiang Yang Rongyu Ping Jiafeng Chen Rui Yang Tianhao Chu Jian Zhou Jia Fan Zheng Tang Dong Yang Yinghong Shi | 2024 | Cancer Communications2024，44，2： | 0 |
| 18 | Effect modifications of BMI transition and trajectory in the associations of adverse childhood experiences with new-onset dementia and its subtypes in older US adults显示文摘Background Adverse childhood experiences(ACEs)and dementia are associated and comorbid with obesity.However,according to emerging research,the role of obesity in the association between ACEs and dementia seems controversial.Aims This analysis aimed to explore the associations between ACEs and different dementia subtypes and the effect modification of long-term body mass index(BMI).Methods Data were obtained from the US Health and Retirement Study.Six ACEs were categorised as 0,1 and 2 or more.All-cause dementia,Alzheimer’s disease(AD)and other dementias were defined by self-reported or proxy-reported physician diagnosis.Cox proportional hazards regression was used to explore the associations of ACEs with new-onset all-cause dementia,AD and other dementias from 2010 to 2020.Effect modification of BMI in 2010 and BMI transition and trajectory(fitted by group-based trajectory modelling)from 2004 to 2010 were assessed.Results 15282 participants with a mean age of 67.0 years(58.0–75.0)were included in the 2010 data analysis.Significant interactions of ACEs with baseline BMI,BMI transition and BMI trajectory in their associations with new-onset all-cause dementia and AD were observed(all p<0.05).For instance,positive associations of two or more ACEs(vs none)with all-cause dementia and AD were found in those with a BMI trajectory of maintaining≥30 kg/m2(maintain obesity)rather than a decline to or maintaining<25 kg/m2(decline to or maintain normal weight),with hazard ratios(HRs)of 1.87(95%confidence interval(CI):1.45 to 2.42)and 1.85(95%CI:1.22 to 2.80),respectively.Conclusions ACEs were associated with dementia and AD in US adults with long-term abnormally elevated BMI but not with long-term normal or decreasing BMI.Integrated weight management throughout life could prevent dementia among those with childhood adversity. | Ziyang Ren Binbin Su Yushan Du Tianjing Zhou Xiaoying Zheng Jufen Liu | 2023 | General Psychiatry2023，36，4： | 0 |
| 19 | Mechanism of Buyin Qianzheng Fang on preventing dopamine neuron from apoptosis by regulating intracellular Ca^(2+) in PD Mice显示文摘The purpose of this study is to investigate the possible protective mechanism of Buyin Qianzheng Fang(BYQZF)on endoplasmic reticular and mitochondrial function through intracellular Ca^(2+) to protect dopamine neurons in Parkinson's disease mice model.C57BL/6 mice were randomly divided into normal group,model group,BYQZF group.The behavior of mice was observed by climbing and beam hang tests. | Zhao Haihong Feng Wandi Ma Haojie Cheng Cuicui Zhou Mengqi Gao Yushan Gai Cong Sun Hongsun | 2021 | 解剖学杂志2021，44，S01： | 0 |
| 20 | Effect of ephedrine hydrochloride on regulation of body fluid metabolism and AQP1 and AQP2 in a rabbit model of mechanical ventilation显示文摘Objective:Ephedrine hydrochloride(EH)is a major component from Ephedra sinica STAPF,which is used as a traditional Chinese herbal medicine.This study was designed to investigate the effect of EH on water metabolism and further explore the relevant signaling pathway of body fluid regulation in“lung governing regulation of water passage”using a rabbit model of mechanical ventilation.The molecular mechanism of the EH effect in the kidney was also investigated.Methods:Rabbits were randomly divided into a control group,model group,EH group,and dexmedetomidine hydrochloride(DH)group.Urine volume was measured by the intubation method and pathologic changes in lung and renal tissue were measured by hematoxylin and eosin staining.Nitric oxide(NO)production in lung,serum,and kidney were analyzed using chemical methods.An ELISA was used to analyze angiotensin II(Ang II),antidiuretic hormone(ADH),prostaglandin E2(PGE2),atrial natriuretic peptide(ANP),and endothelin-1(ET-1)levels in the lung,serum,and kidney.Aquaporin-1(AQP1)and aquaporin-2(AQP2)mRNA and protein expression in the kidney was determined using qRT-PCR and immunohistochemistry.Results:EH significantly inhibited the decrease in the total urine volume in the third and fourth stages,and displayed significant regulatory effects on NO,Ang II,ADH,PGE2,ANP,and ET-1 in serum,lung,and renal tissues compared with the model group.In the kidney,AQP1 and AQP2 mRNA and protein expression in the EH group were remarkably downregulated compared with the model group.Conclusion:EH exerted a regulatory effect on water metabolism by diffusing the lung and increased urine volume in the rabbit model,which was consistent with the decrease in kidney AQP1 and AQP2 expression levels that led to an increase in urine volume.EH could assist with DH to exert a protective effect on the clinical application of mechanical ventilation. | Shujing Zhang Yan Sun Yushan Gao Lu Zhou Zhenyi Wang Xiaolei Liu Pengfei Kang Fengjie Zheng Lei Ni Yuhang Li | 2019 | Journal of Traditional Chinese Medical Sciences2019，6，4： | 0 |
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