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| 1 | Novel Magnesium Alloys Developed for Biomedical Application:A Review显示文摘There is an increasing interest in the development of magnesium alloys both for industrial and biomedical applications.Industrial interest in magnesium alloys is based on strong demand of weight reduction of transportation vehicles for better fuel efficiency,so higher strength,and better ductility and corrosion resistance are required.Nevertheless,biomedical magnesium alloys require appropriate mechanical properties, suitable degradation rate in physiological environment,and what is most important,biosafety to human body. Rather than simply apply commercial magnesium alloys to biomedical field,new alloys should be designed from the point of view of nutriology and toxicology.This article provides a review of state-of-the-art of magnesium alloy implants and devices for orthopedic,cardiovascular and tissue engineering applications. Advances in new alloy design,novel structure design and surface modification are overviewed.The factors that influence the corrosion behavior of magnesium alloys are discussed and the strategy in the future development of biomedical magnesium alloys is proposed. | Nan Li Yufeng Zheng | 2013 | Journal of Materials Science & Technology2013，29，6： | 58 |
| 2 | Biofunctionalization of metallic implants by calcium phosphate coatings显示文摘Metallic materials have been extensively applied in clinical practice due to their unique mechanical properties and durability.Recent years have witnessed broad interests and advances on surface functionalization of metallic implants for high-performance biofunctions.Calcium phosphates(CaPs)are the major inorganic component of bone tissues,and thus owning inherent biocompatibility and osseointegration properties.As such,they have been widely used in clinical orthopedics and dentistry.The new emergence of surface functionalization on metallic implants with CaP coatings shows promise for a combination of mechanical properties from metals and various biofunctions from CaPs.This review provides a brief summary of state-of-art of surface biofunctionalization on implantable metals by CaP coatings.We first glance over different types of CaPs with their coating methods and in vitro and in vivo performances,and then give insight into the representative biofunctions,i.e.osteointegration,corrosion resistance and biodegradation control,and antibacterial property,provided by CaP coatings for metallic implant materials. | Yingchao Su Irsalan Cockerill Yufeng Zheng Liping Tang Yi-Xian Qin Donghui Zhu | 2019 | Bioactive Materials2019，4，1： | 11 |
| 3 | Microstructure, Mechanical Properties, Corrosion Behavior and Biocompatibility of As-Extruded Biodegradable Mg–3Sn–1Zn–0.5Mn Alloy显示文摘The microstructure evolution and mechanical properties of biodegradable Mg–3Sn–1Zn–0.5Mn alloys were investigated by the optical microscopy, X-ray diffractometer and a universal material testing machine.The corrosion and degradation behaviors were studied by potentiodynamic polarization method and immersion test in a simulated body fluid(SBF). It was found that the as-extruded Mg–3Sn–1Zn–0.5Mn alloy has the fine equiaxed grains which underwent complete dynamic recrystallization during the hot extrusion process, with the second phase particles of Mg_2Sn precipitated on the grain boundaries and inside the grains. The tensile strength and elongation of as-extruded Mg–3Sn–1Zn–0.5Mn alloys were 244 ± 3.7 MPa and 19.3% ± 1.7%, respectively. The potentiodynamic polarization curves in SBF solution indicated the better corrosion resistance of the as-extruded Mg–3Sn–1Zn–0.5Mn alloy in the SBF solution. Immersion test in the SBF solution for 720 h revealed that the corrosion rate of as-extruded Mg–3Sn–1Zn–0.5Mn alloy was nearly 4 ± 0.33 mm/year. The hemolysis rate of as-extruded Mg–3Sn–1Zn–0.5Mn alloy was lower than the safe value of 5% according to ISO 10993-4. As-extruded Mg–3Sn–1Zn–0.5Mn alloy showed good biocompatibility after being implanted into the dorsal muscle and the femoral shaft of the rabbit, and no abnormalities were found after short-term implantation. It was revealed that the as-extruded Mg–3Sn–1Zn–0.5Mn alloy is a promising material for biodegradable implants,which possesses an interesting combination of preferred mechanical properties, better corrosion resistance and biocompatibility. | Lida Hou Zhen Li Hong Zhao Yu Pan Sergey Pavlinich Xiwei Liu Xinlin Li Yufeng Zheng Li Li | 2016 | Journal of Materials Science & Technology2016，32，9： | 10 |
| 4 | Regulatory effects of antitumor agent matrine on FOXO and PI3K-AKT pathway in castration-resistant prostate cancer cells显示文摘We previously demonstrated that matrine could inhibit the proliferating, migrating, as well as invading processes of both PC-3 and DU145 cells. However, the underlying molecular mechanisms have not yet been clearly defined. In this study, using various techniques such as high throughput sequencing technology, bioinformatics, quantitative real-time PCR, and immunoblot analysis,we aimed to understand whether matrine serves as a novel regulator of FOXO and PI3K-AKT signaling pathway. DU145 and PC-3 cell lines were cultured for 24 h in vitro. Cells were treated with either matrine or control serum for 48 h, followed by extraction of total RNA. The RNA was sequenced using HiSeq 2500 high-throughput sequencing platform (Illumina). A gene library was established and quality analysis of read data carried out. Integrated database from the website DAVID was used to analyze Gene Ontology (GO), and Kyoto encyclopedia of genes and genomes (KEGG) pathway of differential genes was used for pathway analysis, screening for fold differences of more than two times. The FOXO and PI3K-AKT signaling pathways were screened, and expression levels of mRNA and core protein detected by real-time PCR and immunoblotting, respectively. High throughput sequencing and GO analysis revealed that differentially expressed genes before and after treatment played an important role in cell metabolic process, growth process, anatomical structure formation, cellular component organization, and biological regulation. KEGG signal pathway analysis revealed that FOXO and PI3K-AKT signal pathways had a significant difference between before and after matrine-treated androgen-independent prostate cancer cells PC-3 and DU145. Real-time PCR showed that matrine treatment led to a significant increase in the expression levels of FOXO1A, FOXO3A, FOXO4, and FOXO6 in DU145 and PC-3 cells (P<0.01 or P<0.05), whereas the PI3K expression levels decreased (P<0.01). Similarly, immunoblotting revealed a significant increase (P<0.05) in the expression levels of FOXO1A FOXO3A, FOXO4, and FOXO6 in both PC-3 and DU145 cells, whereas PI3K expression levels decreased (P<0.05). Matrine had a broad regulating effect on the mRNA expression profiles of both PC-3 and DU145 cells. Matrine may inhibit cell proliferation, migration, as well as invasion, and induce apoptosis in both PC-3 and DU145 cells through FOXO and PI3K-AKT signaling pathways. Matrine could therefore be used as a complementary drug to present chemotherapeutic agents, for treating androgen-independent prostate cancer. | Qi Li HaiH uang Zheng He Yi Sun Yufeng Tang Xiaohong Shang Chengbin Wang | 2018 | Science China(Life Sciences)2018，61，5： | 9 |
| 5 | Progress of biodegradable metals显示文摘Biodegradable metals(BMs) are metals and alloys expected to corrode gradually in vivo, with an appropriate host response elicited by released corrosion products, then dissolve completely upon fulfilling the mission to assist with tissue healing with no implant residues. In the present review article, three classes of BMs have been systematically reviewed, including Mg-based, Fe-based and Zn-based BMs.Among the three BM systems, Mg-based BMs, which now have several systems reported the successful of clinical trial results, are considered the vanguards and main force. Fe-based BMs, with pure iron and Fe–Mn based alloys as the most promising, are still on the animal test stage. Zn-based BMs, supposed to have the degradation rate between the fast Mg-based BMs and the slow Fe-based BMs, are a rising star with only several reports and need much further research. The future research and development direction for the BMs are proposed, based on the clinical requirements on controllable degradation rate, prolonged mechanical stability and excellent biocompatibility, by optimization of alloy composition design, regulation on microstructure and mechanical properties, and following surface modification. | Huafang Li Yufeng Zheng Ling Qin | 2014 | Progress in Natural Science:Materials International2014，24，5： | 9 |
| 6 | Research status of biodegradable metals designed for oral and maxillofacial applications:A review显示文摘The oral and maxillofacial regions have complex anatomical structures and different tissue types,which have vital health and aesthetic functions.Biodegradable metals(BMs)is a promising bioactive materials to treat oral and maxillofacial diseases.This review summarizes the research status and future research directions of BMs for oral and maxillofacial applications.Mg-based BMs and Zn-based BMs for bone fracture fixation systems,and guided bone regeneration(GBR)membranes,are discussed in detail.Zn-based BMs with a moderate degradation rate and superior mechanical properties for GBR membranes show great potential for clinical translation.Fe-based BMs have a relatively low degradation rate and insoluble degradation products,which greatly limit their application and clinical translation.Furthermore,we proposed potential future research directions for BMs in the oral and maxillofacial regions,including 3D printed BM bone scaffolds,surface modification for BMs GBR membranes,and BMs containing hydrogels for cartilage regeneration,soft tissue regeneration,and nerve regeneration.Taken together,the progress made in the development of BMs in oral and maxillofacial regions has laid a foundation for further clinical translation. | Dandan Xia Fan Yang Yufeng Zheng Yunsong Liu Yongsheng Zhou | 2021 | Bioactive Materials2021，6，11： | 9 |
| 7 | An overview of graphene-based hydroxyapatite composites for orthopedic applications显示文摘Hydroxyapatite(HA)is an attractive bioceramic for hard tissue repair and regeneration due to its physicochemical similarities to natural apatite.However,its low fracture toughness,poor tensile strength and weak wear resistance become major obstacles for potential clinical applications.One promising method to tackle with these problems is exploiting graphene and its derivatives(graphene oxide and reduced graphene oxide)as nanoscale reinforcement fillers to fabricate graphene-based hydroxyapatite composites in the form of powders,coatings and scaffolds.The last few years witnessed increasing numbers of studies on the preparation,mechanical and biological evaluations of these novel materials.Herein,various preparation techniques,mechanical behaviors and toughen mechanism,the in vitro/in vivo biocompatible analysis,antibacterial properties of the graphene-based HA composites are presented in this review. | Ming Li Pan Xiong Feng Yan Sijie Li Changhong Ren Zhichen Yin Ang Li Huafang Li Xunming Ji Yufeng Zheng Yan Cheng | 2018 | Bioactive Materials2018，3，1： | 9 |
| 8 | Three-dimensional-printed individualized porous implants:A new“implant-bone”interface fusion concept for large bone defect treatment显示文摘Bone defect repairs are based on bone graft fusion or replacement.Current large bone defect treatments are inadequate and lack of reliable technology.Therefore,we aimed to investigate a simple technique using three-dimensional(3D)-printed individualized porous implants without any bone grafts,osteoinductive agents,or surface biofunctionalization to treat large bone defects,and systematically study its long-term therapeutic effects and osseointegration characteristics.Twenty-six patients with large bone defects caused by tumor,infection,or trauma received treatment with individualized porous implants;among them,three typical cases underwent a detailed study.Additionally,a large segmental femur defect sheep model was used to study the osseointegration characteristics.Immediate and long-term biomechanical stability was achieved,and the animal study revealed that the bone grew into the pores with gradual remodeling,resulting in a long-term mechanically stable implant-bone complex.Advantages of 3D-printed microporous implants for the repair of bone defects included 1)that the stabilization devices were immediately designed and constructed to achieve early postoperative mobility,and 2)that osseointegration between the host bone and implants was achieved without bone grafting.Our osseointegration method,in which the“implant-bone”interface fusion concept was used instead of“bone-bone”fusion,subverts the traditional idea of osseointegration. | Teng Zhang Qingguang Wei Hua Zhou Zehao Jing Xiaoguang Liu Yufeng Zheng Hong Cai Feng Wei Liang Jiang Miao Yu Yan Cheng Daoyang Fan Wenhao Zhou Xinhong Lin Huijie Leng Jian Li Xinyu Li Caimei Wang Yun Tian Zhongjun Liu | 2021 | Bioactive Materials2021，6，11： | 9 |
| 9 | Biodegradable Zn-Sr alloy for bone regeneration in rat femoral condyle defect model: In vitro and in vivo studies显示文摘Bone defects are commonly caused by severe trauma,malignant tumors,or congenital diseases and remain among the toughest clinical problems faced by orthopedic surgeons,especially when of critical size.Biodegradable zinc-based metals have recently gained popularity for their desirable biocompatibility,suitable degradation rate,and favorable osteogenesis-promoting properties.The biphasic activity of Sr promotes osteogenesis and inhibits osteoclastogenesis,which imparts Zn-Sr alloys with the ideal theoretical osteogenic properties.Herein,a biodegradable Zn-Sr binary alloy system was fabricated.The cytocompatibility and osteogenesis of the Zn-Sr alloys were significantly better than those of pure Zn in MC3T3-E1 cells.RNA-sequencing illustrated that the Zn-0.8Sr alloy promoted osteogenesis by activating the wnt/β-catenin,PI3K/Akt,and MAPK/Erk signaling pathways.Furthermore,rat femoral condyle defects were repaired using Zn-0.8Sr alloy scaffolds,with pure Ti as a control.The scaffold-bone integration and bone ingrowth confirmed the favorable in vivo repair properties of the Zn-Sr alloy,which was verified to offer satisfactory biosafety based on the hematoxylin-eosin(H&E)staining and ion concentration testing of important organs.The Zn-0.8Sr alloy was identified as an ideal bone repair material candidate,especially for application in critical-sized defects on load-bearing sites due to its favorable biocompatibility and osteogenic properties in vitro and in vivo. | Bo Jia Hongtao Yang Zechuan Zhang Xinhua Qu Xiufeng Jia Qiang Wu Yu Han Yufeng Zheng Kerong Dai | 2021 | Bioactive Materials2021，6，6： | 9 |
| 10 | Optimizing mechanical property and cytocompatibility of the biodegradable Mg-Zn-Y-Nd alloy by hot extrusion and heat treatment显示文摘The Mg-Zn-Y-Nd alloy is a new type of degradable material for biomedical application. In the present study, Mg-6Zn-1.2Y-0.8Nd alloy was fabricated, and then extrusion and heat treatment were conducted to optimize its mechanical properties and cytocompatibility. The microstructure observation, mechanical property, degradation behavior and cytocompatibility tests were conducted on the Mg-Zn-Y-Nd alloy with three different states: as-cast(alloy C), as-extruded(alloy E) and extruded + heat treated(alloy EH).The results show that alloy C consists of coarse grains and continuous secondary phases. The extrusion process has caused incomplete recrystallization, and results in a mixed grain structure of elongated grains and small equiaxed grains(alloy E). The heat treatment process has promoted the recrystallization and homogenized the grain structure(alloy EH). Both the strength and ductility of the alloy has been improved by extrusion, but the following heat treatment has decreased the strength and increased the ductility.The degradation behavior of the alloy C and E alloys does not show much difference, but improves slightly in alloy EH, because the heat treatment has homogenized the microstructure and released the residual stress in the alloy. The directly and indirectly cell viability tests indicate that alloy EH exhibits the best cytocompatibility, which should be ascribed to its relative uniform degradation and low ion releasing rate. In summary, the combination of hot extrusion and heat treatment could optimize the mechanical property and cytocompatibility of the Mg-Zn-Y-Nd alloy together, which is beneficial for the future application of the alloy. | Yiyuan Kang Beining Du Yueming Li Baojie Wang Liyuan Sheng Longquan Shao Yufeng Zheng Tingfei Xi | 2019 | Journal of Materials Science & Technology2019，35，1： | 8 |
| 11 | Multifunctional MgF_2/Polydopamine Coating on Mg Alloy for Vascular Stent Application显示文摘Mg alloy is of great potential in the application of vascular stent due to its degradation in physical environment and proper mechanical property.However its mechanical integrity does not meet the clinical requirement due to relatively fast degradation.Besides,in order to accelerate the reendothelialization of Mg-based stents,it needs surface modification to improve the attachment,growth and adhesion of endothelial cells(ECs).To solve the main obstacles,an anti-corrosion and quick endothelialization coating was prepared on novel Mg-Zn-Y-Nd alloy via a simple two-step immersion method in the present study,first in hydrofluoric acid(HF) then in dopamine tris-Hydrochloric acid(tris-HC1) solution.The coating was uniform and thin,which consisted of two layers—the upper was polydopamine(PDA) layer and the lower was MgF_2 layer.The alloy with the coating demonstrated dramatic corrosion resistance enhancement in vitro by immersion test and electrochemical test.Moreover the HFPDA-treated Mg alloy exhibited great performance of cell adhesion and proliferation.The coating created a favorable environment for ECs to have a competitive advantage over vascular smooth muscle cells(VSMCs),which was preferable for re-endothelialization.The results suggest that HF-PDA-treated Mg-Zn-Y-Nd alloy has great potential in the application of vascular stent and the surface coating method is of great application value in biodegradable Mg alloy stent due to its simplicity and effectiveness. | Xiaoli Liu Zhen Zhen Jing Liu Tingfei Xi Yudong Zheng Shaokang Guan Yufeng Zheng Yan Cheng | 2015 | Journal of Materials Science & Technology2015，31，7： | 8 |
| 12 | Corrosion of magnesium and magnesium–calcium alloy in biologically-simulated environment显示文摘A study of biocompatibility and corrosion of both metallic magnesium(Mg) and a magnesium alloy containing 1% calcium(Mg–Ca) were investigated in in vitro culture conditions with and without the presence of bone marrow derived human mesenchymal stem cells(h MSCs).Chemical analysis of the degraded samples was performed using XRD and FEGSEM. The results from the XRD analysis strongly suggested that crystalline phase of magnesium carbonate was present on the surface of both the Mg and Mg–Ca samples. Flame absorption spectrometry was used to analyse the release of magnesium and calcium ions into the cell culture medium. Magnesium concentration was kept consistently at a level ranging from 40 to 80 m M for both Mg and Mg–Ca samples. No cell growth was observed when in direct contact with the metals apart from a few cells observed at the bottom of culture plate containing Mg–Ca alloy. In general, in vitro study of corrosion of Mg–Ca in a biologicallysimulated environment using cell culture medium with the presence of h MSCs demonstrated close resemblances to in vivo corrosion. Although in vitro corrosion of Mg–Ca revealed slow corrosion rate and no immediate cytotoxicity effects to h MSCs, its corrosion rate was still too high to achieve normal stem cell growth when cells and alloys were cultured in vitro in direct contact. | Richard Harrison Diana Maradze Simon Lyons Yufeng Zheng Yang Liu | 2014 | Progress in Natural Science:Materials International2014，24，5： | 8 |
| 13 | In vitro degradation and biocompatibility evaluation of typical biodegradable metals (Mg/Zn/Fe) for the application of tracheobronchial stenosis显示文摘Tracheobronchial obstruction in children due to benign stenosis or tracheobronchomalacia still remains a challenging matter of concern.Currently,there is 10%–20%complication rate in clinical treatment.The nonbiodegradable property of silicone stents and nickel-titanium memory alloy stents take the primary responsibility for drawbacks including stimulating local granulation tissue proliferation,displacement,and stent-related infections.Permanent tracheobronchial stent will be a persistent foreign object for a long time,causing excessive secretion of tracheal mucosa,ulceration and even perforation,which is particularly unsuitable for young children with persistent tracheal growth.In this study,the degradation and biocompatibility performance of three typical biodegradable metals were investigated as potential tracheobronchial stent materials.The results exhibited that these materials showed different degradation behaviors in the simulating respiratory fluid environment compared with SBF.Except for pure iron group,high purity magnesium and zinc showed favorable cell adhesion and proliferation in three culture methodologies(direct culture,indirect culture and extraction culture).The proper corrosion rate and good biocompatibility indicated that high purity magnesium and zinc may be good candidates as tracheobronchial stent materials. | Yangyang Li Jianglong Yan Wenhao Zhou Pan Xiong Pei Wang Wei Yuan Yufeng Zheng Yan Cheng | 2019 | Bioactive Materials2019，4，1： | 8 |
| 14 | In vitro and in vivo biodegradation and biocompatibility of an MMT/BSA composite coating upon magnesium alloy AZ31显示文摘The performance of biodegradable magnesium alloy requires special attention to rapid degradation and poor biocompatibility, which can cause the implant to fail. Here, a sodium montmorillonite(MMT)/bovine serum albumin(BSA) composite coating was prepared upon magnesium alloy AZ31 via hydrothermal synthesis, followed by dip coating. We evaluated the surface characterization and corrosion behavior in vitro, and the biocompatibility in vitro and in vivo. Biodegradation progress of the MMT-BSA coated Mg pieces was examined through hydrogen evolution, immersion tests, and electrochemical measurements in Hank’s solution. In vitro biocompatibility studies were evaluated via hemolysis tests, dynamic cruor time tests, platelet adhesion, MTT testing and live-dead stain of osteoblast cells(MC3 T3-E1). It was found that the MMT-BSA coating had good corrosion resistance and a marked improvement in biocompatibility in comparison to bare Mg alloy AZ31. in vivo studies were carried out in rat model and the degradation was characterized by computed tomography scans. Results revealed that the MMT-BSA coated Mg alloy AZ31 implants maintained their structural integrity and slight degradation after 120 d of post-implantation. A100% survival rate for the rats was observed with no obvious toxic damages on the organs and tissues.Additionally, we proposed a sound coating formation mechanism. Considering the good corrosion protection and biocompatibility, the MMT-BSA coated Mg alloy AZ31 is a promising candidate material for biomedical implants. | Jian Wang Lanyue Cui Yande Ren Yuhong Zou Jinlong Ma Chengjian Wang Zhongyin Zheng Xiaobo Chen Rongchang Zeng Yufeng Zheng | 2020 | Journal of Materials Science & Technology2020，47，12： | 7 |
| 15 | Niobium pentoxide:a promising surface-enhanced Raman scattering active semiconductor substrate显示文摘Surface-enhanced Raman scattering technique,as a powerful tool to identify the molecular species,has been severely restricted to the noble metals.The surface-enhanced Raman scattering substrates based on semiconductors would overcome the shortcomings of metal substrates and promote development of surface-enhanced Raman scattering technique in surface science,spectroscopy,and biomedicine studies.However,the detection sensitivity and enhancement effects of semiconductor substrates are suffering from their weak activities.In this work,a semiconductor based on Nb_(2)O_(5) is reported as a new candidate for highly sensitive surfaceenhanced Raman scattering detection of dye molecules.The largest enhancement factor value greater than 107 was observed with the laser excitation at 633 and 780 nm for methylene blue detection.As far as literature review shows,this is in the rank of the highest sensitivity among semiconductor materials;even comparable to the metal nanostructure substrates with“hot spots”.The impressive surface-enhanced Raman scattering activities can be attributed to the chemical enhancement dominated by the photoinduced charge transfer,as well as the electromagnetic enhancement,which have been supported by the density-functional-theory and finite element method calculation results.The chemisorption of dye on Nb_(2)O_(5) creates a new highest occupied molecular orbital and lowest unoccupied molecular orbital contributed by both fragments in the molecule-Nb_(2)O_(5) system,which makes the charge transfer more feasible with longer excitation wavelength.In addition,the electromagnetic enhancement mechanism also accounts for two orders of magnitude enhancement in the overall enhancement factor value.This work has revealed Nb_(2)O_(5) nanoparticles as a new semiconductor surface-enhanced Raman scattering substrate that is able to replace noble metals and shows great potentials applied in the fields of biology related. | Yufeng Shan Zhihui Zheng Jianjun Liu Yong Yang Zhiyuan Li Zhengren Huang Dongliang Jiang | 2017 | npj Computational Materials2017，，1： | 7 |
| 16 | Serum zinc levels and multiple health outcomes: Implications for zinc-based biomaterials显示文摘Background:Zinc-based biomaterials,including biodegradable metal,nanoparticles,and coatings used in medical implants release zinc ions that may increase the whole-body and serum zinc concentrations.The impact of serum zinc concentrations on major health outcomes can provide insights for device design and clinical transformation of zinc-based biomaterials.Methods:This nationally representative cross-sectional study enrolled participants from the National Health and Nutrition Examination Survey(NHANES,2011-2014)including 3607 participants.Using unadjusted and multivariate-adjusted logistic regression analyses,two-piecewise linear regression model with a smoothing function and threshold level analysis,we evaluated the associations between elevated serum zinc levels and major health outcomes.Results:Elevated serum zinc levels were significantly associated with an increase in total spine and total femur bone mineral density(BMD).Every 10μg/dL increase was associated with a 1.12-fold increase in diabetes mellitus(DM)and 1.23-fold and 1.29-fold increase in cardiovascular diseases(CVD)and coronary heart disease(CHD),in participants with serum zinc levels≥100μg/dL.It had no significant linear or nonlinear associations with risk of fractures,congestive heart failure,heart attack,thyroid disease,arthritis,osteoarthritis,rheumatoid arthritis,dyslipidemia and cancer.Conclusion:Serum zinc levels are significantly associated with increased BMD in the total spine and total femur,and risk of DM,and CVD/CHD among participants with serum zinc levels≥100μg/dL. | Xinhua Qu Hongtao Yang Zhifeng Yu Bo Jia Han Qiao Yufeng Zheng Kerong Dai | 2020 | Bioactive Materials2020，5，2： | 7 |
| 17 | In Vitro Study on Mg-Sn-Mn Alloy as Biodegradable Metals显示文摘The mechanical properties,chemical properties and biocompatibility of Mg-3Sn—0.5Mn alloy were tested.A series of in vitro evaluations such as tensile test,static and dynamic immersion test,hemocompatibility test as well as cytotoxicity test were presented,with commercial magnesium alloy WE43 as the control.Mg-3Sn-0.5Mn alloy possesses suitable strength and superior ductility compared with WE43 and AZ31.Static immersion and dynamic degradation tests showed more uniform degradation with a more moderate rate for Mg—3Sn—0.5Mn alloy(0.34 mm/y in static condition and 0.25 mm/y in dynamic condition)compared with WE43 alloy(0.42 mm/y in static condition and 0.33 mm/y in dynamic condition) in Hank's solution.Blood compatibility evaluation suggested that Mg—3Sn—0.5Mn alloy had no destructive effect on erythrocyte and showed excellent anti-thrombogenicity to blood system.Besides,Mg—3Sn—0.5Mn alloy showed no inhibition effect to L929 metabolic activity and mild toxicity to vascular smooth muscle cell(VSMC) in preliminary cell viability assessment.By considering its excellent mechanical strength,corrosion resistance,low ion release rate and good biocompatibility,Mg—3Sn—0.5Mn alloy may be a promising economical candidate as biomedical implant material for load-bearing clinical applications in the future. | Zhen Zhen Tingfei Xi Yufeng Zheng Li Li Lugee Li | 2014 | Journal of Materials Science & Technology2014，30，7： | 6 |
| 18 | Melatonin enhances radiofrequency-induced NK antitumor immunity,causing cancer metabolism reprogramming and inhibition of multiple pulmonary tumor development显示文摘Surgery is the comm on treatme nt for early lung cancer with multiple pulm onary no dules,but it is often accompanied by the problem of significant malignancy of other nodules in non-therapeutic areas.In this study,we found that a combined treatment of local rad iofreq ue ncy ablati on(RFA)and melatonin(MLT)greatly improved clinical outcomes for early lung cancer patie nts with multiple pulmonary nodules by minimizing lung function injury and reducing the probability of malignant transformation or enlargement of nodules in non-ablated areas.Mechanically,as demonstrated in an associated mouse lung tumor model,RFA not only effectively remove treated tumors but also stimulate antitumor immunity,which could inhibit tumor growth in non-ablated areas.MLT enhanced RFA-stimulated NK activity and exerted synergistic antitumor effects with RFA.Transcriptomics and proteomics analyses of residual tumor tissues revealed enhanced oxidative phosphorylation and reduced acidification as well as hypoxia in the tumor microenvironment,which suggests reprogrammed tumor metabolism after combined treatment with RFA and MLT.Analysis of residual tumor further revealed the depressed activity of MAPK,NF-kappa B,Wnt,and Hedgehog pathways and upregulated P53 pathway in tumors,which was in line with the inhibited tumor growth.Combined RFA and MLT treatment also reversed the Warburg effect and decreased tumor malignancy.These findings thus demonstrated that combined treatment of RFA and MLT effectively inhibited the malignancy of non-ablated nodules and provided an innovative non-invasive strategy for treating early lung tumors with multiple pulmonary nodules.Trial registration:www.chictr.org.cn,identifier ChiCTR2100042695,http://gffzz62a03ee25e6f4a77hwcuxpv569o9w6665.ffgz.tsg.suse.edu.cn/showproj.aspx?proj=120931. | Ming Li Bingjie Hao Menghuan Zhang Russel J.Reiter Shumeng Lin Tiansheng Zheng Xiangyun Chen Yanbei Ren Liduo Yue Baigenzhin Abay Guojie Chen Xiao Xu Yufeng Shi Lihong Fan | 2021 | Signal Transduction and Targeted Therapy2021，6，10： | 6 |
| 19 | CD147 antibody specifically and effectively inhibits infection and cytokine storm of SARS-CoV-2 and its variants delta,alpha,beta,and gamma显示文摘SARS-CoV-2 mutations contribute to increased viral transmissibility and immune escape,compromising the effectiveness of existing vaccines and neutralizing antibodies.An in-depth investigation on COVID-19 pathogenesis is urgently needed to develop a strategy against SARS-CoV-2 variants.Here,we identified CD147 as a universal receptor for SARS-CoV-2 and its variants.Meanwhile,Meplazeumab,a humanized anti-CD147 antibody,could block cellular entry of SARS-CoV-2 and its variants-alpha,beta,gamma,and delta,with inhibition rates of 68.7,75.7,52.1,52.1,and 62.3%at 60μg/ml,respectively.Furthermore,humanized CD147 transgenic mice were susceptible to SARS-CoV-2 and its two variants,alpha and beta.When infected,these mice developed exudative alveolar pneumonia,featured by immune responses involving alveoli-infiltrated macrophages,neutrophils,and lymphocytes and activation of IL-17 signaling pathway.Mechanistically,we proposed that severe COVID-19-related cytokine storm is induced by a'spike protein-CD147-CyPA signaling axis':Infection of SARS-CoV-2 through CD147 initiated the JAK-STAT pathway,which further induced expression of cyclophilin A(CyPA);CyPA reciprocally bound to CD147 and triggered MAPK pathway.Consequently,the MAPK pathway regulated the expression of cytokines and chemokines,which promoted the development of cytokine storm.Importantly,Meplazumab could effectively inhibit viral entry and inflammation caused by SARS-CoV-2 and its variants.Therefore,our findings provided a new perspective for severe COVID-19-related pathogenesis.Furthermore,the validated universal receptor for SARS-CoV-2 and its variants can be targeted for COVID-19 treatment. | Jiejie Geng Liang Chen Yufeng Yuan Ke Wang Youchun Wang Chuan Qin Guizhen Wu Ruo Chen Zheng Zhang Ding Wei Peng Du Jun Zhang Peng Lin Kui Zhang Yongqiang Deng Ke Xu Jiangning Liu Xiuxuan Sun Ting Guo Xu Yang Jiao Wu Jianli Jiang Ling Li Kun Zhang Zhe Wang Jing Zhang Qingguo Yan Hua Zhu Zhaohui Zheng Jinlin Miao Xianghui Fu Fengfan Yang Xiaochun Chen Hao Tang Yang Zhang Ying Shi Yumeng Zhu Zhuo Pei Fei Huo Xue Liang Yatao Wang Qingyi Wang Wen Xie Yirong Li Mingyan Shi Huijie Bian Ping Zhu Zhi-Nan Chen | 2021 | Signal Transduction and Targeted Therapy2021，6，10： | 6 |
| 20 | Electrophoretic-deposited novel ternary silk fibroin/graphene oxide/hydroxyapatite nanocomposite coatings on titanium substrate for orthopedic applications显示文摘 | Ming LI Pan XIONG Maosong MO Yan CHENG Yufeng ZHENG | 2016 | Frontiers of Materials Science2016，10，3： | 6 |
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