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| 1 | Induction of Th1-Type Immune Response by Chitosan Nanoparticles Containing Plasmid DNA Encoding House Dust Mite Allergen Der p2 for Oral Vaccination in Mice显示文摘This study was to prepare the chitosan-pDer p 2 nanoparticles and to investigate the effect of chitosan-DNA nanoparticles on immune response in mice by oral delivery of chitosan-DNA nanoparticles. The nanoparticles were synthesized by complexing chitosan with plasmid DNA. The DNA was fully complexed into chitosan-DNA nanoparticles,suggesting a 100% encapsulation efficiency. Chitosan-DNA complex renders a significant protection of the plasmid. No effect on cell viability was observed in both cell types and average cell viability over 100% was obtained. Oral gene delivery with chitosan-DNA nanoparticles can generate a higher level expression of gene in vivo. Oral chitosan-pDer p 2 nanoparticles in BALB/c mice can induce IFN-γ in serum and prevent subsequent sensitization of Th2 cell-regulated specific IgE responses. The data indicate that the oral administration of chitosan-pDer p 2 nanoparticles results in the expression of Der p 2 in the epithelial cells of both stomach and small intestine and the induction of Th1-type immune response. | Guoping Li Zhigang Liu Bin Liao Nanshan Zhong | 2009 | Cellular & Molecular Immunology2009，6，1： | 10 |
| 2 | Therapeutic Effects of DNA Vaccine on Allergen-Induced Allergic Airway Inflammation in Mouse Model显示文摘Vaccination with DNA encoding Dermatophagoides pteronyssinus group 2 (Der p 2) allergen previously showed its effects of immunologic protection on Der p 2 allergen-induced allergic airway inflammation in mice. In present study, we investigated whether DNA vaccine encoding Der p 2 could exert therapeutic role on allergen-induced allergic airway inflammation in mouse model and explored the mechanism of DNA vaccination in asthma specific-allergen immunotherapy. After sensitized and challenged by Der p 2, the BALB/c mice were immunized with DNA vaccine. The degrees of cellular infiltration were scored. IgE levels in serum and IL-4/IL-13 levels in BALF were determined by ELISA. The lung tissues were assessed by histological examinations. Expressions of STAT6 and NF-κB in lung were determined by immunohistochemistry staining. Vaccination of mice with DNA vaccine inhibited the development of airway inflammation and the production of mucin induced by allergen, and reduced the level of Der p 2-specific IgE level. Significant reductions of eosinophil infiltration and levels of IL-4 and IL-13 in BALF were observed after vaccination. Further more, DNA vaccination inhibited STAT6 and NF-κB expression in lung tissue in Der p 2-immunized mice. These results indicated that DNA vaccine encoding Der p 2 allergen could be used for therapy of allergen-induced allergic airway inflammation in our mouse model. | Guoping Li Zhigang Liu Nanshan Zhong Bin Liao Ying Xiong | 2006 | Cellular & Molecular Immunology2006，3，5： | 9 |
| 3 | Nucleation field,reversal mechanism and coercivity paradox in two-phased magnetic nanosystem显示文摘The magnetic reversal mechanism has been determined within a micromagnetic model reliably for a two-phased magnetic nanosystem,with the formulae for nucleation fields derived analytically.It is found that the nucleation field HN decreases uniformly as the size of the soft phase Ls increases whereas it increases with the size of the hard phase Lh.The analysis shows that whilst the effect of Lh could be ignored in most cases,where the nucleation field is dominated by the Ls and the calculation could be significantly simplified,the overly simple inverse square law between HN and the soft layer thickness Ls adopted by the previous researchers is not a good approximation.While nucleation is the beginning of the magnetic reversal,pinning is the dominant coercivity mechanism in both two-phased and single-phased magnetic materials,where the crystalline defects exist.Comparison with the experimental data confirms this conclusion,indicating that Brown's paradox results from the much lower effective anisotropy in both single-phased and composite materials,as speculated in the literature. | ZHAO GuoPing ZHANG HuaiWu ZHONG ZhiYong CHEN Lang | 2011 | Science China(Physics,Mechanics & Astronomy)2011，54，7： | 3 |
| 4 | Subtyping of type A influenza by sequencing the variable regions of HA gene specifically amplified with RT-PCR显示文摘The outbreak of a novel influenza A(H1N1) virus across the globe poses a threat to human health.It is of paramount importance to develop a rapid,reliable and inexpensive diagnostic procedure.Based on the bioinformatic information from public database,primers specific for influenza A virus surface protein haemagglutinin(HA) of several subtypes(including H1,H2,H3,H5,H7 and H9) were designed.Primer-specific PCR products were subjected to sequencing for accurately distinguishing H1 and H3 subtypes from others.This sequencing-based detection method will not only be applied to rapid detection and simultaneous subtype identification of new influenza A virus H1N1,but also provide the strategies to monitor other new types ofinfluenza virus with explosive potential. | YAN An DING GuoHui ZHOU ZhenFeng DONG Hui ZHANG YaKun ZHU Lei HE YunGang ZHANG GuoQing LI YiXue SUN Bing HUANG Zhong LAN Ke JIN Li WANG HongYan WANG XiaoNing YANG Zhong ZHONG Yang DAI JianXin GUO YaJun WANG Hao CHE XiaoYan WU Fan YUAN ZhenGan ZHANG Xi CAO ZhiWei ZHOU XiaoNong ZHOU JiaHai MA ZhiYong TONG GuangZhi ZHAO GuoPing JIN WeiRong XIONG Hui | 2009 | Chinese Science Bulletin2009，54，13： | 3 |
| 5 | The mutation network for the hemagglutinin gene from the novel influenza A (H1N1) virus显示文摘A mutation network for the hemagglutinin gene(HA) of the novel type A(H1N1) influenza virus was constructed.Sequence homology analysis indicated that one HA sequence type from the viruses mainly isolated from Mexico was likely the original type in this epidemic.Based on the 658A and 1408T mutations in HA,the viruses evolving into this epidemic were divided into three categories,the Mexico,the transitional and the New York type.The three groups of viruses presented distinctive clustering features in their geographic distributions. | HE YunGang DING GuoHui BIAN Chao HUANG Zhong LAN Ke SUN Bing WANG XueCai LI YiXue WANG HongYan WANG XiaoNing YANG Zhong ZHONG Yang JIN WeiRong XIONG Hui DAI JianXin GUO YaJun WANG Hao CHE XiaoYan WU Fan YUAN ZhenAn ZHANG Xi CAO ZhiWei ZHOU XiaoNong ZHOU JiaHai MA ZhiYong TONG GuangZhi ZHAO GuoPing JIN Li | 2009 | Chinese Science Bulletin2009，54，13： | 3 |
| 6 | An analyzing and experimental method based on the resultant motion signals for SCARA manipulator joints显示文摘Acceleration reflects vibration of a robot,and the vibration signal can reflect the operation state of the robot. Generally,detection of robot mechanical arm failure requires installing sensors on each joint. This study proposes a method to diagnose the fault by single acceleration sensor only,which is installed at the end of the robot. The operation state of the robot is evaluated by analyzing vibration characteristics of its acceleration. First,a data acquisition function of a programmable multi-axis controller is applied to extract practical motion signals of the robot joints during operation,and practical motion signals are analyzed. Second,synthetic methods to determine acceleration of the end joints of SCARA robots in a Cartesian space is used based on the theory of the Jacobian matrix and the frequency domain of final acceleration is investigated. The relationship between end-and joint-vibration frequencies under given speeds is determined. Then,the method is verified by comparing characteristic frequencies of joint acceleration and synthetic acceleration in Cartesian coordinate system at different speeds. Finally,some faults can be diagnosed by comparing the acceleration vibration frequency extracted by a single acceleration sensor installed at the end of robot with the normal running state. Thus,this method can be used to monitor the signal variation of each joint without installing sensors on each robot joint. | 徐丰羽 Yang Zhong Jiang Guoping | 2017 | High Technology Letters2017，23，3： | 3 |
| 7 | Learn to fight from the war显示文摘In March 2009,a novel type A (H1N1) influenza broke out in North America and rapidly spread all over the world. | ZHAO GuoPing Shanghai-MOST Key Laboratory for Disease and Health Genomics,Chinese National Human Genome Center at Shanghai,Shanghai 201203,China Lab of Synthetic Biology,Shanghai Institutes of Biological Sciences,Chinese Academy of Sciences,Shanghai 200031,China National Engineering Research Center for Biochip Technology,Shanghai Biochip Co.Ltd.,Shanghai 201203,China School of Life Sciences,Fudan University,Shanghai 200433,China ZHONG Yang School of Life Sciences,Fudan University,Shanghai 200433,China Shanghai Center for Bioinformation Technology,Shanghai 200235,China | 2009 | Chinese Science Bulletin2009，54，13： | 2 |
| 8 | Structure modeling and spatial epitope analysis for HA protein of the novel H1N1 influenza virus显示文摘In recent months,a novel influenza virus H1N1 broke out around the world.With bioinformatics technology,the 3D structure of HA protein was obtained,and the epitope residues were predicted with the method developed in our group for this novel flu virus.58 amino acids were identified as potential epitope residues,the majority of which clustered at the surface of the globular head of HA protein.Although it is located at the similar position,the epitope of HA protein for the novel H1N1 flu virus has obvious differences in the electrostatic potential compared to that of HA proteins from previous flu viruses. | WU Di XU TianLei SUN Jing DAI JianXin DING GuoHui HE YunGang ZHOU ZhengFeng XIONG Hui DONG Hui JIN WeiRong BIAN Chao JIN Li WANG HongYan WANG XiaoNing YANG Zhong ZHONG Yang WANG Hao CHE XiaoYan HUANG Zhong LAN Ke SUN Bing WU Fan YUAN ZhenAn ZHANG Xi ZHOU XiaoNong ZHOU JiaHai MA ZhiYong TONG GuangZhi GUO YaJun ZHAO GuoPing LI YiXue CAO ZhiWei | 2009 | Chinese Science Bulletin2009，54，13： | 2 |
| 9 | Symptom clustering in chronic gastritis based on spectral clustering显示文摘OBJECTIVE: Apply spectral clustering to analyze the patterns of symptoms in patients with chronic gastritis(CG).METHODS: Based on 919 CG subjects, we applied mutual information feature selection to choose the positively correlated symptoms with each pattern.Then, we used the Shi and Malik spectral clustering algorithm to select the top 20 correlated symptoms.RESULTS: We ascertained the results of six patterns.There were three categories for the pattern of accumulation of damp heat in the spleen-stomach(0.00332). There were six categories for the pattern of dampness obstructing the spleen-stomach(0.02466). There were two categories for the pattern of spleen-stomach Qi deficiency(0.013 89).There were three categories for the pattern of spleen-stomach deficiency cold(0.009 15). There were five categories for the pattern of liver-Qistagnation(0.01910).There were four categories for the pattern of stagnant heat in the liver-stomach(0.00585).CONCLUSION: Most of the spectral clustering results of the symptoms of CG patterns were in accordance with clinical experience and Traditional Chinese Medicine theory. Most categories suggested the nature and/or location of the disease. | Wenhua Zhu Zhaoxiang Fan Guoping Liu Jianjun Yan Tao Zhong Wu Zheng Ruiqing Wang Chunying Wang | 2014 | Journal of Traditional Chinese Medicine2014，34，4： | 2 |
| 10 | Leptospira interrogans encodes an ROK family glucokinase involved in a cryptic glucose utilization pathway显示文摘尽管螺旋体 interrogans 是不能的作为它的碳 / 精力利用葡萄糖,采购原料, L 的 LA_1437 基因。interrogans serovar Lai 潜在地编码组 III glucokinase (GLK ) 。L。interrogans GLK (LiGLK ) 不同类地在 Escherichia 表示了 coli 被净化并且证明了是有它 12.3 的特定的活动的 homodimeric 酶 ? 桴 ? 慰瑲慩?? 潤慭湩愠摮渠牯慭?? 整浲湩獵? 獩猠潨瑲牥琠慨 ? 偁 ? 湡 ??? 戠 ? 桴 ? 敲獡湯漠 ? 敧敮映慲敭桳晩 ? 畭慴楴湯搠牵湩? | Qing Zhang Yunyi Zhang Yi Zhong Jun Ma Nanqiu Peng Xingjun Cao Chen Yang RongZeng Xiaokui Guo Guoping Zhao | 2011 | Acta Biochimica et Biophysica Sinica2011，43，8： | 1 |
| 11 | Longitudinal virological changes and underlying pathogenesis in hospitalized COVID-19 patients in Guangzhou,China显示文摘Prolonged viral RNA shedding and recurrence of severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)in coronavirus disease 2019(COVID-19)patients have been reported.However,the clinical outcome and pathogenesis remain unclear.In this study,we recruited 43 laboratory-confirmed COVID-19 patients.We found that prolonged viral RNA shedding or recurrence mainly occurred in severe/critical patients(P<0.05).The average viral shedding time in severe/critical patients was more than 50 days,and up to 100 days in some patients,after symptom onset.However,chest computed tomography gradually improved and complete absorption occurred when SARS-CoV-2 RT-PCR was still positive,but specific antibodies appeared.Furthermore,the viral shedding time significantly decreased when the A1,430G or C12,473T mutation occurred(P<0.01 and FDR<0.01)and increased when G227A occurred(P<0.05 and FDR<0.05).High IL1R1,IL1R2,and TNFRSF21 expression in the host positively correlated with viral shedding time(P<0.05 and false discovery rate<0.05).Prolonged viral RNA shedding often occurs but may not increase disease damage.Prolonged viral RNA shedding is associated with viral mutations and host factors. | Zhengtu Li Yinhu Li Ruilin Sun Shaoqiang Li Lingdan Chen Yangqing Zhan Mingzhou Xie Jiasheng Yang Yanqun Wang Airu Zhu Guoping Gu Le Yu Shuaicheng Li Tingting Liu Zhaoming Chen Wenhua Jian Qian Jiang Xiaofen Su Weili Gu Liyan Chen Jing Cheng Jincun Zhao Wenju Lu Jinping Zheng Shiyue Li Nanshan Zhong Feng Ye | 2021 | Science China(Life Sciences)2021，64，12： | 1 |
| 12 | Expression of Both Matrix Metalloproteinase-2 and Its Tissue Inhibitor-2 in Tunica Media of Radial Artery in Uremic Patients显示文摘 | Aiqin Shan Canxin Zhou Yongping He Weisheng Feng Gun Chen Guoping Zhong | 2013 | Renal Failure2013，，1： | 1 |
| 13 | Linkage analysis suggests a locus of ichthyosis vulgaris on 1q22显示文摘 | Wei Zhong Bin Cui Yizhi Zhang Haisong Jiang Shengcai Wei Lei Bu Guoping Zhao Landian Hu Xiangyin Kong | 2003 | Journal of Human Genetics2003，，7： | 1 |
| 14 | Interleukin-6 expression was regulated by epigenetic mechanisms in response to influenza virus infection or dsRNA treatment显示文摘 | Bikui Tang Ruihua Zhao Yong Sun Ying Zhu Jiang Zhong Guoping Zhao Naishuo Zhu | 2011 | Molecular Immunology2011，，8： | 1 |
| 15 | Decolorization of anthraquinone dye by Shewanella decolorationis S12显示文摘 | Meiying Xu Jun Guo Guoqu Zeng Xiaoyan Zhong Guoping Sun | 2006 | Applied Microbiology and Biotechnology2006，，2： | 1 |
| 16 | TRANSPARENT TESTA 2 regulates embryonic fatty acid biosynthesis by targeting FUSCA 3 during the early developmental stage of A rabidopsis seeds显示文摘 | Zhong Wang Mingxun Chen Tianlong Chen Lijie Xuan Zhilan Li Xue Du Longhua Zhou Guoping Zhang Lixi Jiang | 2014 | Plant J2014，，5： | 1 |
| 17 | Simultaneous determination of metformin and gliclazide in human plasma by liquid chromatography - tandem mass spectrometry : application to a bioequivalence study of two formulations in healthy volunteers显示文摘 | ZHONG Guoping BI Huichang ZHOU Shufeng | 2005 | Journal of Mass Spectrometer2005，40，11： | 1 |
| 18 | Three new labdane-type diterpene glycosides from fruits of Rubus chingii and their cytotoxic activities againstfive humor cell lines显示文摘 | Ruijian Zhong Qing Guo Guoping Zhou | 2015 | Fitoterapia2015，，102： | 1 |
| 19 | Vitamin C Mitigates Oxidative Stress and Tumor Necrosis Factor-Alpha in Severe Community-Acquired Pneumonia and LPS-Induced Macrophages显示文摘 | Yuanyuan Chen Guangyan Luo Jiao Yuan Yuanyuan Wang Xiaoqiong Yang Xiaoyun Wang Guoping Li Zhiguang Liu Nanshan Zhong Fábio Santos de Lira | 2014 | Mediators of Inflammation2014，，： | 1 |
| 20 | A stability locomotion-control strategy for quadruped robots with center-of-mass dynamic planning显示文摘Locomotion stability is essential for controlling quadruped robots and adapting them to unstructured terrain.We propose a control strategy with center-of-mass(CoM)dynamic planning for the stable locomotion of these robots.The motion trajectories of the swing legs are synchronized with the CoM of the robot.To implement the synchronous control scheme,we adjusted the swing legs to form a support triangle.The strategy is applicable to both static walk gait and dynamic trot gait.In the motion control processes of the robot legs,the distribution of the ground reaction forces is optimized to minimize joint torque and locomotion energy consumption.We also used an improved joint-torque controller with varied controller coefficients in the stance and swing phases.The simulation and experimental results demonstrate that the robot can complete omnidirectional locomotion in both walk and trot gaits.At a given locomotion speed,the stability margins for the robot during walking and trotting were 27.25%and 37.25%higher,respectively,than in the scheme without CoM planning.The control strategy with energy consumption optimization(ECO)reduced the energy consumption of the robot in walk and trot gaits by 11.25%and 13.83%,respectively,from those of the control scheme without ECO. | Yangyang HAN Guoping LIU Zhenyu LU Huaizhi ZONG Junhui ZHANG Feifei ZHONG Liyu GAO | 2023 | Journal of Zhejiang University-Science A(Applied Physics & Engineering)2023，24，6： | 0 |
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