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| 1 | Dysfunction of peripheral blood dendritic cells from patients with chronic hepatitis B virus infection显示文摘AIM To identify the property of dendritic cella (DCs) of peripheral blood monocytes (PBMC) in patlents with chronic HBV infection.METHODS Twenty patients with persistent HBV infectlon were included in this study, 10 healthy subjects being used as a control group. The peripheral blood mononuclear cells (PBMC) of T cell-depleted populations were incubated and induced into mature dendritic cells in the RPMI-1640 medium in the presence of cytokines GMCSF, IL-4, FLt-3, TNF-α and 100 mL@ L-1 of fetal calf serum for a total of 10 - 12 days. The expressions of surface markers on DCs were evaluated using flow cytometric analysis. ELISA method was used to determine the cytokine levels of interleukin-12 (IL-12) and IL-10 in the supernatant produced by DCs. For detection of the stimulatory capacity of DCs to T cell proliferation,mytomycin C-treated DC were incubated with allogenic T cells.RESULTS A typical morphology of mature DCs from healthy subjects and HBV-infected patients was induced in in vitro incubation, but the proliferation ability and cellular number of DCs from HBV-infected patients significantly decreased compared with healthy individuals. In particular, the expression levels of HLADR, CD80 (B7-1) and CD86 (B7-2) on DC surface from patients were also lower than that from healthy individuals (0.46 vs 0.92 for HLA-DR, 0.44 vs 0.88 for CD80 and 0.44 vs 0. 84 for CD86, P< 0.05). The stimulatory capacity and production of IL-12 of DCs from patients in allogenic mixed lymphocyte reaction (AMLR) significantly decreased, but the production level of nitric oxide (NO) by DCa simultaneously increased compared with healthy subjects (86± 15 vs 170±22 μmoI@L 1, P<0.05).CONCLUSION The patients with chronic HBV infection have the defective function and immature phenotype of dendritic cells, which may be associated with the inability of efficient presentation of HBV antigens to host immune system for the clearance of HBV. | Fu-Sheng Wang Li-He Xing Ming-Xu Liu Chuan-Lin Zhu Hui-Gang Liu Hui-Fen Wang Zhou-Yun Lei Division of Biological Engineering,~2 Fourth Department of Liver Diseases,Beijing Institute of Infectious Diseases,Beijing Hospital of Infectious Diseases,Beijing 100039,China | 2001 | World Journal of Gastroenterology2001，7，4： | 131 |
| 2 | A complete sequence and comparative analysis of a SARS-associated virus(Isolate BJ01)显示文摘The genome sequence of the Severe Acute Respiratory Syndrome (SARS)-associated virus provides essential information for the identification of pathogen(s), exploration of etiology and evolution, interpretation of transmission and pathogenesis, development of diagnostics, prevention by future vaccination, and treatment by developing new drugs. We report the complete genome sequence and comparative analysis of an isolate (BJ01) of the coronavirus that has been recognized as a pathogen for SARS. The genome is 29725 nt in size and has 11 ORFs (Open Reading Frames). It is composed of a stable region encoding an RNA-dependent RNA polymerase (composed of 2 ORFs) and a variable region representing 4 CDSs (coding sequences) for viral structural genes (the S, E, M, N proteins) and 5 PUPs (putative uncharacterized proteins). Its gene order is identical to that of other known coronaviruses. The sequence alignment with all known RNA viruses places this virus as a member in the family of Coronaviridae. Thirty putative substitutions have been identified by comparative analysis of the 5 SARS- associated virus genome sequences in GenBank. Fifteen of them lead to possible amino acid changes (non-synonymous mutations) in the proteins. Three amino acid changes, with predicted alteration of physical and chemical features, have been detected in the S protein that is postulated to beinvolved in the immunoreactions between the virus and its host. Two amino acid changes have been detected in the Mprotein, which could be related to viral envelope formation. Phylogenetic analysis suggests the possibility of non-human origin of the SARS-associated viruses but provides noevidence that they are man-made. Further efforts should focus on identifying the etiology of the SARS-associated virus and ruling out conclusively the existence of otherpossible SARS-related pathogen(s). | QIN E'de ZHU Qingyu YU Man FAN Baochang CHANG Guohui SI Bingyin YANG Bao PENG Wenming JIANG Tao LIU Bohua DENG Yongqiang LIU Hong ZHANG Yu WANG Cui LI Yuquan GAN Yonghua LI Xiaoyu L Fushuang TAN Gang CAO Wuchun, YANG Ruifu Institute of Microbiology and Epidemiology, Chinese Academy of Military Medical Sciences, Beijing 100071, China WANG Jian, LI Wei, XU Zuyuan, LI Yan, WU Qingfa, LIN Wei, CHEN Weijun, TANG Lin, DENG Yajun, HAN Yujun, LI Changfeng, LEI Meng, LI Guoqing, LI Wenjie, L Hong, SHI Jianping, TONG Zongzhong, ZHANG Feng, LI Songgang, LIU Bin, LIU Siqi, DONG Wei, WANG Jun, Gane K-S Wong, YU Jun & YANG Huanming* Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300 National Center for Genome Information, Beijing 101300, China | 2003 | Chinese Science Bulletin2003，48，10： | 121 |
| 3 | New knowledge of the mechanisms of sorafenib resistance in liver cancer显示文摘Sorafenib 是压制肿瘤房间增长和 angiogenesis 并且支持肿瘤房间 apoptosis 的一个口头的 multikinase 禁止者。它被 FDA 在 2006 为先进肾的房间癌的治疗同意,并且作为为在 2007 的先进 hepatocellular 癌(HCC ) 的唯一的目标药。Sorafenib 能显著地扩大病人,但是仅仅由 3-5 月的中部的幸存时间。而且,它与抵抗经常开发的严肃的不利副作用,和药被联系。因此,探索位于 sorafenib 抵抗下面的机制并且为应付这些问题开发个性化的治疗学的策略是很重要的。最近的研究表明了除了主要抵抗,几机制正在位于获得的抵抗下面到 sorafenib,例如包含 PI3K/Akt 和 JAK-STAT 小径,组织缺氧可诱导的小径的激活,和上皮间充质的转变的串音。这里,我们简短为药抵抗描述 sorafenib,它的临床的应用程序,和分子的机制的函数,特别为 HCC 病人。 | Yan-jing ZHU Bo ZHENG Hong-yang WANG Lei CHEN | 2017 | Acta Pharmacologica Sinica2017，38，5： | 65 |
| 4 | 2018 Chinese Pediatric Cardiology Society(CPCS) guideline for diagnosis and treatment of syncope in children and adolescents显示文摘Syncope belongs to the transient loss of consciousness(TLOC), characterized by a rapid onset, short duration, and spontaneous complete recovery. It is common in children and adolescents, accounting for 1% to 2% of emergency department visits.Recurrent syncope can seriously affect children's physical and mental health, learning ability and quality of life and sometimes cardiac syncope even poses a risk of sudden death. The present guideline for the diagnosis and treatment of syncope in children and adolescents was developed for guiding a better clinical management of pediatric syncope. Based on the globally recent development and the evidence-based data in China, 2018 Chinese Pediatric Cardiology Society(CPCS) guideline for diagnosis and treatment of syncope in children and adolescents was jointly prepared by the Pediatric Cardiology Society, Chinese Pediatric Society, Chinese Medical Association(CMA)/Committee on Pediatric Syncope, Pediatricians Branch, Chinese Medical Doctor Association(CMDA)/Committee on Pediatric Cardiology, Chinese College of Cardiovascular Physicians, Chinese Medical Doctor Association(CMDA)/Pediatric Cardiology Society, Beijing Pediatric Society, Beijing Medical Association(BMA). The present guideline includes the underlying diseases of syncope in children and adolescents, the diagnostic procedures, methodology and clinical significance of standing test and headup tilt test, the clinical diagnosis vasovagal syncope, postural orthostatic tachycardia syndrome, orthostatic hypotension and orthostatic hypertension, and the treatment of syncope as well as follow-up. | Cheng Wang Yaqi Li Ying Liao Hong Tian Min Huang Xiangyu Dong Lin Shi Jinghui Sun Hongfang Jin Junbao Du Jindou An Jie Chen Mingwu Chen Qi Chen Sun Chen Yonghong Chen Zhi Chen Adolphus Kai-tung Chau Junbao Du Zhongdong Du Junkai Duan Hongyu Duan Xiangyu Dong Lin Feng Lijun Fu Fangqi Gong Yonghao Gui Ling Han Zhenhui Han Bing He Zhixu He Xiufen Hu Yimin Hua Guoying Huang Min Huang Ping Huang Yujuan Huang Hongfang Jin Mei Jin Bo Li Fen Li Tao Li Xiaohui Li Xiaoyan Liu Yan Li Haitao Lv Tiewei Lv Zipu Li Luyi Ma Silin Pan Yusheng Pang Hua Peng Yuming Qin Jie Shen Lin Shi Kun Sun Jinghui Sun Hong Tian Jie Tian Cheng Wang Hong Wang Lei Wang Jinju Wang Wendi Wang Yuli Wang Rongzhou Wu Tianhe Xia Yanyan Xiao Chunhong Xie Yanlin Xing Zhenyu Xiong Baoyuan Xu Yi Xu Hui Yan Shiwei Yang Qijian Yi Xia Yu Xianyi Yu Yue Yuan Hongyan Zhang Huili Zhang Li Zhang Qingyou Zhang Xi Zhang Yanmin Zhang Zhiwei Zhang Cuifen Zhao Bin Zhou Hua Zhu | 2018 | Science Bulletin2018，63，23： | 54 |
| 5 | Persistence and clearance of viral RNA in 2019 novel coronavirus disease rehabilitation patients显示文摘Background:A patient’s infectivity is determined by the presence of the virus in different body fluids,secretions,and excreta.The persistence and clearance of viral RNA from different specimens of patients with 2019 novel coronavirus disease(COVID-19)remain unclear.This study analyzed the clearance time and factors influencing 2019 novel coronavirus(2019-nCoV)RNA in different samples from patients with COVID-19,providing further evidence to improve the management of patients during convalescence.Methods:The clinical data and laboratory test results of convalescent patients with COVID-19 who were admitted to from January 20,2020 to February 10,2020 were collected retrospectively.The reverse transcription polymerase chain reaction(RT-PCR)results for patients’oropharyngeal swab,stool,urine,and serum samples were collected and analyzed.Convalescent patients refer to recovered non-febrile patients without respiratory symptoms who had two successive(minimum 24 h sampling interval)negative RT-PCR results for viral RNA from oropharyngeal swabs.The effects of cluster of differentiation 4(CD4)+T lymphocytes,inflammatory indicators,and glucocorticoid treatment on viral nucleic acid clearance were analyzed.Results:In the 292 confirmed cases,66 patients recovered after treatment and were included in our study.In total,28(42.4%)women and 38 men(57.6%)with a median age of 44.0(34.0-62.0)years were analyzed.After in-hospital treatment,patients’inflammatory indicators decreased with improved clinical condition.The median time from the onset of symptoms to first negative RT-PCR results for oropharyngeal swabs in convalescent patients was 9.5(6.0-11.0)days.By February 10,2020,11 convalescent patients(16.7%)still tested positive for viral RNA from stool specimens and the other 55 patients’stool specimens were negative for 2019-nCoV following a median duration of 11.0(9.0-16.0)days after symptom onset.Among these 55 patients,43 had a longer duration until stool specimens were negative for viral RNA than for throat swabs,with a median delay of 2.0(1.0-4.0)days.Results for only four(6.9%)urine samples were positive for viral nucleic acid out of 58 cases;viral RNA was still present in three patients’urine specimens after throat swabs were negative.Using a multiple linear regression model(F=2.669,P=0.044,and adjusted R2=0.122),the analysis showed that the CD4+T lymphocyte count may help predict the duration of viral RNA detection in patients’stools(t=-2.699,P=0.010).The duration of viral RNA detection from oropharyngeal swabs and fecal samples in the glucocorticoid treatment group was longer than that in the non-glucocorticoid treatment group(15 days vs.8.0 days,respectively;t=2.550,P=0.013)and the duration of viral RNA detection in fecal samples in the glucocorticoid treatment group was longer than that in the non-glucocorticoid treatment group(20 days vs.11 days,respectively;t=4.631,P<0.001).There was no statistically significant difference in inflammatory indicators between patients with positive fecal viral RNA test results and those with negative results(P>0.05).Conclusions:In brief,as the clearance of viral RNA in patients’stools was delayed compared to that in oropharyngeal swabs,it is important to identify viral RNA in feces during convalescence.Because of the delayed clearance of viral RNA in the glucocorticoid treatment group,glucocorticoids are not recommended in the treatment of COVID-19,especially for mild disease.The duration of RNA detection may relate to host cell immunity. | Yun Ling Shui-Bao Xu Yi-Xiao Lin Di Tian Zhao-Qin Zhu Fa-Hui Dai Fan Wu Zhi-Gang Song Wei Huang Jun Chen Bi-Jie Hu Sheng Wang En-Qiang Mao Lei Zhu Wen-Hong Zhang Hong-Zhou Lu | 2020 | Chinese Medical Journal2020，，9： | 58 |
| 6 | Long-term efficacy of perioperative chemoradiotherapy on esophageal squamous cell carcinoma显示文摘AIM: To investigate the role of perioperative chemoradiotherapy (CRT) in the treatment of locally advanced thoracic esophageal squamous cell carcinoma (ESCC). METHODS: Using preoperative computed tomography (CT)-based staging criteria, 238 patients with ESCC (stage ⅡⅢ ) were enrolled in this prospective study between January 1997 and June 2004. With informed consent, patients were randomized into 3 groups: preoperative CRT (80 cases), postoperative CRT (78 cases) and surgery alone (S) (80 cases). The 1-, 3-, 5and 10-year survival were followed up. Progressionfree survival (PFS) was chosen as the primary endpoint by treatment arm measured from study entry until documented progression of disease or death from any cause. The secondary endpoint was overall survival (OS) determined as the time (in months) between the date of therapy and the date of death. Other objectives were surgical and adjuvant therapy complications.RESULTS: With median follow-up of 45 mo for all the enrolled patients, significant differences in the 1-, 3-, 5-, 10-year OS (91.3%, 63.5%, 43.5%, 24.5% vs 91%, 62.8%, 42.3%, 24.4% vs 87.5%, 51.3%, 33.8%, 12.5%, P = 0.0176) and PFS (89.3%, 61.3%, 37.5%, 18.1% vs 89.1%, 61.1%, 37.2%, 17.8% vs 84.5%, 49.3%, 25.9%, 6.2%, P = 0.0151) were detected among the 3 arms. There were no significant differences in OS and PFS between the preoperative CRT and postoperative CRT arm (P > 0.05). For the patients who had radical resection, significant differences in median PFS (48 mo vs 61 mo vs 39.5 mo, P = 0.0331) and median OS (56.5 mo vs 72 mo vs 41.5 mo, P = 0.0153) were detected among the 3 arms, but there were no significant differences in OS and PFS between the preoperative CRT and postoperative CRT arm (P > 0.05). The local recurrence rates in the preoperative CRT, postoperative CRT group and S group were 11.3%, 14.1% and 35%, respectively (P < 0.05). No significant differences were detected among the 3 groups when comparing complications but tended to be in favor of the postoperative CRT and S groups (P > 0.05). Toxicities of CRT in the preoperative or postoperative CRT arms were mostly moderate, and could be quickly alleviated by adequate therapy. CONCLUSION: Rational application of preoperative or postoperative CRT can provide a benefit in PFS and OS in patients with locally advanced ESCC. | Lv, Jin Cao, Xiu-Feng Zhu, Bin Ji, Lv Tao, Lei Wang, Dong-Dong | 2010 | World Journal of Gastroenterology2010，16，13： | 49 |
| 7 | Comparative Study between Robotic Total Thyroidectomy with Central Lymph Node Dissection via Bilateral Axillo-breast Approach and Conventional Open Procedure for Papillary Thyroid Microcarcinoma显示文摘 | Qing-Qing He Jian Zhu Da-Yong Zhuang Zi-Yi Fan Lu-Ming Zheng Peng Zhou Lei Hou Fang Yu Yan-Ning Li Lei Xiao Xue-Feng Dong Gao-Feng Ni | 2016 | Chinese Medical Journal2016，，18： | 42 |
| 8 | Preventive effect of ulinastatin and gabexate mesylate on post-endoscopic retrograde cholangiopancreatography pancreatitis显示文摘背景 endoscopic 以后后退 cholangiopancreatography 胰腺炎(锐气) 被认为是之一使人烦恼的复杂并发症内视镜后退 cholangiopancreatography (ERCP ) 。在锐气上评估 ulinastatin 和 gabexate mesylate (美国通用汽车公司) 的预防效果的使随机化的控制试用的结果是矛盾的。现在的学习被设计与使随机化的控制试用(RCT ) 的元分析在锐气上评估 ulinastatin 和美国通用汽车公司的预防效果 .Methods 五个电子数据库被在找在锐气上评估 ulinastatin 和美国通用汽车公司的预防效果的 RCT。概括效果被估计,方法由 Cochrane Collaboration.Results 推荐了包含 5105 个参加者的 12 研究在我们的元分析被包括。ulinastatin 的管理减少了仅仅在足够的剂量的锐气的发生(或, 0.39;95% CI, 0.19 ~ 0.81;P=0.01 ) 。需要到对待(NNT ) 的数字是 6。并且 ulinastatin 的管理也减少了 post-ERCP hyperamylasemia (PEHA ) 的发生(或, 0.40;95% CI, 0.28 ~ 0.58;P < 0.000 01 ) 。高剂量的美国通用汽车公司的慢注入为锐气预防是有效的(或, 0.44; 95% CI, 0.25 ~ 0.79;P=0.006 ) ,并且低剂量的美国通用汽车公司的快速的注入也为锐气预防显示出功效(或, 0.37; 95% CI, 0.20 ~ 0.69;P=0.002 ) 。NNT 分别地是 7 和 6。然而,在低剂量并且由慢注入的美国通用汽车公司的管理是无效的(或, 0.99;95% CI, 0.64 ~ 1.55;P=0.98 ) 。美国通用汽车公司的管理有趋势减少 PEHA 率,然而并非到统计意义(或, 0.86;95% CI, 0.73 ~ 1.01;P=0.06 ) 。当低质量的研究被排除时,有二高质量的研究的元分析显示 ulinastatin 没减少锐气的率(或, 0.63;95% CI, 0.32 ~ 1.26;P=0.19 ) 并且 PEHA 发生(或, 0.80;95% CI, 0.31 ~ 2.07;P=0.64 ) 。有六高质量的研究的元分析证明美国通用汽车公司管理减少了锐气发生(或, 0.52;95% CI, 0.29 ~ 0.91; P=0.02 ) 不为 PEHA 预防是有效的(或, 0.88;95% CI, 0.74 ~ 1.04;P=0.12 ).Conclusions Ulinastatin 和美国通用汽车公司可能具有为锐气的预防的价值。美国通用汽车公司应该在高剂量并且由快速的注入被管理。并且 ulinastatin 的剂量应该是足够的。然而,结论不是 overwhelming.More 大样品的尺寸和高质量的 RCT 仍然被需要阐明二药的政府是否确实穿上预防效果锐气。 | ZHANG Zhi-feng YANG Ning ZHAO Gang ZHU Lei ZHU Ying WANG Li-xia | 2010 | Chinese Medical Journal2010，，18： | 38 |
| 9 | Transplantation of collagen scaffold with autologous bone marrow mononuclear cells promotes functional endometrium reconstruction via downregulating ΔNp63 expression in Asherman's syndrome显示文摘Asherman's syndrome(AS) is a common disease that presents endometrial regeneration disorder. However, little is known about its molecular features of this aregenerative endometrium in AS and how to reconstruct the functioning endometrium for the patients with AS. Here, we report that ΔNp63 is significantly upregulated in residual epithelial cells of the impaired endometrium in AS; the upregulated-ΔNp63 induces endometrial quiescence and alteration of stemness. Importantly, we demonstrate that engrafting high density of autologous bone marrow mononuclear cells(BMNCs) loaded in collagen scaffold onto the uterine lining of patients with AS downregulates ΔNp63 expression, reverses ΔNp63-induced pathological changes, normalizes the stemness alterations and restores endometrial regeneration. Finally, five patients achieved successful pregnancies and live births. Therefore, we conclude that ΔNp63 is a crucial therapeutic target for AS. This novel treatment significantly improves the outcome for the patients with severe AS. | Guangfeng Zhao Yun Cao Xianghong Zhu Xiaoqiu Tang Lijun Ding Haixiang Sun Juan Li Xinan Li Chenyan Dai Tong Ru Hui Zhu Jingjie Lu Caimei Lin Jingmei Wang Guijun Yan Huiyan Wang Lei Wang Yimin Dai Bin Wang Ruotian Li Jianwu Dai Yan Zhou Yali Hu | 2017 | Science China(Life Sciences)2017，60，4： | 39 |
| 10 | Reprogramming of ovine adult fibroblasts to pluripotency via drug-inducible expression of defined factors显示文摘在 enucleated 鸡蛋的体的房间的 Reprogramming 做了多丽,绵羊,在 1996 的第一个成功地克隆的哺乳动物。然而,绵羊的机制体的房间 reprogramming 还没被探讨了。而且,绵羊胚胎的茎(ES ) 细胞仍然不是可得到的,它限制精确修改基因的绵羊的产生。在这研究,我们报导体的房间能是的那只绵羊直接 reprogrammed 到导致的 pluripotent 用定义因素(Oct4, Sox2, c-Myc, Klf4, Nanog, Lin28, SV40 大 T 和 hTERT ) 的茎(iPS ) 房间。我们的观察显示从绵羊的体的细胞比从另外的种,在 iPS,细胞被报导了的体的细胞对改编更困难。我们证明绵羊 iPS 房间表示 ES 房间标记,包括碱的磷酸酶, Oct4, Nanog, Sox2, Rex1,阶段特定的胚胎的 antigen-1, TRA-1-60, TRA-1-81 和 E-cadherin。绵羊 iPS 房间展出了正常 karyotypes 并且能在 vitro 并且在 teratomas 区分进所有三细菌层。我们的学习可以帮助在绵羊揭示体的房间 reprogramming 的机制并且提供一个平台为绵羊 ES 房间探索文化条件。而且,绵羊 iPS 房间可以直接被用来产生精确修改基因的绵羊。 | Lei Bao Lixiazi He Jijun Chen Zhao Wu Jing Liao Lmgdun Rao Jiangtao Ren Hui Li Hui Zhu Lei Qian Yijun Gu Huimin Dai Xun Xu Jinqiu Zhou Wen Wang Chun Cui Lei Xiao | 2011 | Cell Research2011，21，4： | 32 |
| 11 | Study of time-domain digital pulse shaping algorithms for nuclear signals显示文摘With the development on high-speed integrated circuit,fast high resolution sampling ADC and digital signal processors are replacing analog shaping amplifier circuit.This paper firstly presents the numerical analysis and simulation on R-C shaping circuit model and C-R shaping circuit model.Mathematic models are established based on 1st order digital differential method and Kirchhoff Current Law in time domain,and a simulation and error evaluation experiment on an ideal digital signal are carried out with Excel VBA.A digital shaping test for a semiconductor X-ray detector in real time is also presented.Then a numerical analysis for Sallen-Key(S-K) low-pass filter circuit model is implemented based on the analysis of digital R-C and digital C-R shaping methods.By applying the 2nd order non-homogeneous differential equation,the authors implement a digital Gaussian filter model for a standard exponential-decaying signal and a nuclear pulse signal.Finally,computer simulations and experimental tests are carried out and the results show the possibility of the digital pulse processing algorithms. | ZHOU Jianbin ZHOU Wei LEI Jiarong TUO Xianguo ZHU Xing LIU Yi | 2012 | Nuclear Science and Techniques2012，23，3： | 34 |
| 12 | Experimental Study of the Pressure Fluctuations in a Pump Turbine at Large Partial Flow Conditions显示文摘Frequent shifts of output and operating mode require a pump turbine with excellent stability. Current researches show that large partial flow conditions in pump mode experience positive-slope phenomena with a large head drop. The pressure fluctuation at the positive slope is crucial to the pump turbine unit safety. The operating instabilities at large partial flow conditions for a pump turbine are analyzed. The hydraulic performance of a model pump turbine is tested with the pressure fluctuations measured at unstable operating points near a positive slope in the performance curve. The hydraulic performance tests show that there are two separated positive-slope regions for the pump turbine, with the flow discharge for the first positive slope from 0.85 to 0.91 times that at the maximum efficiency point. The amplitudes of the pressure fluctuations at these unstable large partial flow conditions near the first positive slope are much larger than those at stable operating condtions. A dominant frequency is measured at 0.2 times the impeller rotational frequency in the flow passage near the impeller exit, which is believed to be induced by the rotating stall in the flow passage of the wicket gates. The test results also show hysteresis with pressure fluctuations when the pump turbine is operated near the first positive slope. The hysteresis creates different pressure fluctuations for those operation points even though their flow rates and heads are similar respectively. The pressure fluctuation characteristics at large partial flow conditions obtained by the present study will be helpful for the safe operation of pumped storage units. | RAN Hongjuan LUO Xianwu ZHU Lei ZHANG Yao WANG Xin XU Hongyuan | 2012 | Chinese Journal of Mechanical Engineering2012，25，6： | 33 |
| 13 | Smartphone Application WeChat for Clinical Follow-up of Discharged Patients with Head and Neck Tumors: A Randomized Controlled Trial显示文摘 | Ke-Xing Lyu Jing Zhao Bin Wang Guan-Xia Xiong Wei-Qiang Yang Qi-Hong Liu Xiao-Lin Zhu Wei Sun Ai-Yun Jiang Wei-Ping Wen Wen-Bin Lei | 2016 | Chinese Medical Journal2016，，23： | 31 |
| 14 | Development of a Haploid-Inducer Mediated Genome Editing System for Accelerating Maize Breeding显示文摘Crop breeding aims to generate pure in bred lines with multiple desired traits. Doubled haploid (DH) and genome editing using CRISPR/Cas9 are two powerful game-changing technologies in crop breeding. However, both of them still fall short for rapid generation of pure elite lines with integrated favorable traits. Here, we report the development of a Haploid-Inducer Mediated Genome Editing (IMGE) approach, which utilizes a maize haploid inducer line carrying a CRISPR/Cas9 cassette targeting for a desired agronomic trait to pollinate an elite maize in bred line and to generate genome-edited haploids in the elite maize background. Homozygous pure DH lines with the desired trait improvement could be generated within two generations, thus bypassing the lengthy procedure of repeated crossing and backcrossing used in conventional breeding for integrating a desirable trait into elite commercial backgrounds. | Baobao Wang Lei Zhu Binbin Zhao Yongping Zhao Yurong Xie Zhigang Zheng Yaoyao Li Juan Sun Haiyang Wang | 2019 | Molecular Plant2019，12，4： | 32 |
| 15 | Identification and characterization of microRNAs in raw milk during different periods of lactation, commercial fluid, and powdered milk products显示文摘最近的婴儿的公式奶粉污染事件证明了在牛奶质量控制的经典标记或标准在识别鈥渕a nipulated 鈥 ? 是不够的差质量的牛奶。在现在的学习,我们第一次证明奶牛牛奶包含大量 microRNAs ( miRNAs )并且牛奶特定的 miRNAs 的唯一的表示侧面能为未加工的牛奶和牛奶相关的商业产品的质量控制用作新奇指示物和可能的新标准,例如液体牛奶和粉状的公式牛奶。用定序的 Solexa,首先,我们系统地在未加工的牛奶屏蔽了 miRNA 表示并且在未加工的牛奶识别了 245 miRNAs 的一个总数。不同于层次在哺乳的不同时期是其表达式将近相同的另外的经典 biomarkers,单个 miRNAs 能显著地在哺乳过程期间被改变,含有那 miRNAs 可以是更精确的指示物反映牛奶的优秀改变。第二,使用 TaqMan 基于探查的 miRNA 量的 RT-PCR ,我们进一步识别了七 miRNAs 重要地在整个哺乳过程,和更多有相对一致的表情,这七牛奶特定的 miRNAs 的表示侧面能为区别用作理想的 biomarker 差质量或从纯未加工的牛奶的 鈥渕a nipulated 鈥?牛奶,象为商业乳制品的质量控制一样,例如液体,牛奶和粉状的公式挤。一起,我们的调查结果为为决定未加工的牛奶或牛奶相关的商业产品的质量理解牛奶 miRNAs 和一个新潜在的标准的生理的角色提供一个基础。 | Xi Chen Chao Gao Haijin Li Lei Huang Qi Sun Yanye Dong Chunliang Tian Shengpu Gao Hailin Dong Danping Guan Xiaoyun Hu Shujian Zhao Liang Li Lin Zhu Qiao Yan Junfeng Zhang Ke Zen Chen-Yu Zhang | 2010 | Cell Research2010，20，10： | 29 |
| 16 | m^6A mRNA methylation sustains Treg suppressive functions显示文摘 | Jiyu Tong Guangchao Cao Ting Zhang Esen Sefik Maria Carolina Amezcua Vesely James P Broughton Shu Zhu Huabin Li Bin Li Lei Chen Howard Y Chang Bing Su Richard A Flavell Hua-Bing Li | 2018 | Cell Research2018，28，2： | 30 |
| 17 | Stenting for symptomatic intracranial arterial stenosis in China:1-year outcome of a multicentre registry study显示文摘background and purpose A multicentre prospective registry study of individually tailored stenting for a patient with symptomatic intracranial atherosclerotic stenosis(ICAS)combined with poor collaterals in China showed that the short-term safety and efficacy of stenting was acceptable.However,it remained uncertain whether the low event rate could be of a long term.We reported the 1-year outcome of this registry study to evaluate the long-term efficacy of individually tailored stenting for patients with severe symptomatic ICAS combined with poor collaterals.Methods Patients with symptomatic ICAS caused by 70%-99% stenosis located at the intracranial internal carotid,middle cerebral,intracranial vertebral or basilar arteries combined with poor collaterals were enrolled.Balloon-mounted stent or balloon plus self-expanding stent were selected based on the ease of vascular access and lesion morphology determined by the operators.The primary outcome was the rate of 30-day stroke,transient ischaemic attack and death,and 12-month ischaemic stroke within the same vascular territory,haemorrhagic stroke and vascular death after stenting.results From September 2013 to January 2015,300 patients(ages 58.3±9.78 years)were recruited.Among them,159 patients were treated with balloon-mounted stent and 141 with balloon plus self-expanding stent.During the 1-year follow-up,25 patients had a primary end point event.The probability of primary outcome at 1 year was 8.1%(95% CI 5.3% to 11.7%).In 76 patients with digital subtraction angiography follow-up,27.6%(21/76)had re-stenosis≥50% and 18.4%(14/76)had re-stenosis≥70%.No baseline characteristic was associated with the primary outcome.Conclusion The event rate remains low over 1 year of individually tailored stenting for patients with severe symptomatic ICAS combined with poor collaterals.Further randomised trial of comparing individually tailored stenting with best medical therapy is needed. | Ning Ma Yong Zhang Jie Shuai Changchun Jiang Qiyi Zhu Kangning Chen Li Liu Baomin Li Xiangqun Shi Lianbo Gao Yajie Liu Feng Wang Yongli Li Tieyan Liu Hongbo Zheng Dapeng Mo Feng Gao Yilong Wang Yongjun Wang Lei Feng Zhongrong Miao | 2018 | Stroke & Vascular Neurology2018，3，3： | 29 |
| 18 | Zircon U-Pb geochronological framework of Qitianling granite batholith, middle part of Nanling Range, South China显示文摘The Qitianling granite batholith (QGB) is located in the southern Hunan Province, middle part of the Nanling Range, South China. Its total exposure area is about 520 km2. Based on our 25 single grain zircon U-Pb age data and 7 published data as well as the geological, petrological, and space distribution characteristics, we conclude that QGB is an Early Yanshanian (Jurassic) multi-staged composite pluton. Its formation process can be subdivided into three major stages. The first stage, emplaced at 163―160 Ma with a peak at about 161 Ma, is mainly composed of hornblende-biotite monzonitic granites and locally biotite granites, and distributed in the eastern, northern, and western peripheral parts of the pluton. The second stage, emplaced at 157―153 Ma with a peak at 157―156 Ma, is mainly composed of biotite granites and locally containing hornblende, and distributed in the middle and southeastern parts of the pluton. The third stage, emplaced at 150―146 Ma with a peak at about 149 Ma, is mainly composed of fine-grained (locally porphyritic) biotite granites, and distributed in the middle-southern part of the pluton. Each stage can be further disintegrated into several granite bodies. The first two intrusive stages comprise the major phase of QGB, and the third intrusive stage comprises the additional phase. Many second stage fine-grained granite bosses and dykes intruded into the first stage host granites with clear chilling margin-baking phenomena at their intrusive contacts. They were emplaced in the open fracture space of the earlier stage consolidated rocks. Their isotopic ages are mostly 2―6 Ma younger than their hosts. Conceivably, the time interval from magma emplacement, through cooling, crystallization, solidification, up to fracturing of the earlier stage granites cannot exceed 2―6 Ma. During the Middle-Late Jurassic in the Qitianling area and neighboring Nanling Range, the coeval granitic and basic-intermediate magmatic activities were widely developed. It indicates that the Early Yanshanian period was the culmination time of magmatic activities in this region. The Nanling Range was under a post-orogenic, intracontinental geotectonic environment with an obvious lithospheric extension and thinning. The crust-mantle interaction played an important role in formation of granitic rocks in this region. | ZHU JinChu WANG RuCheng ZHANG PeiHua XIE CaiFu ZHANG WenLan ZHAO KuiDong XIE Lei YANG Ce CHE XuDong YU APeng WANG LuBin | 2009 | Science China Earth Sciences2009，52，9： | 27 |
| 19 | Effect of neoadjuvant chemoradiotherapy on prognosis and surgery for esophageal carcinoma显示文摘AIM:To investigate the role of neoadjuvant chemoradiotherapy in prognosis and surgery for esophageal carcinoma by a meta-analysis.METHODS:PubMed and manual searches were done to identify all published randomized controlled trials(RCTs) that compared neoadjuvant chemoradiotherapy plus surgery(CRTS) with surgery alone(S) for esophageal cancer.According to the test of heterogeneity,a fi xed-effect model or a random effect model was used and the odds ratio(OR) was the principal measure of effects.RESULTS:Fourteen RCTs that included 1737 patients were selected with quality assessment ranging from A to C(Cochrane Reviewers' Handbook 4.2.2).OR(95% CI,P value),expressed as CRTS vs S(values>1 favor CRTS arm),was 1.19(0.94-1.48,P=0.28) for 1-year survival,1.33(1.07-1.65,P=0.69) for 2-year survival,1.76(1.42-2.19,P=0.11) for 3-year survival,1.41(1.06-1.87,P=0.11) for 4-year survival,1.64(1.28-2.12,P=0.40) for 5-year survival,0.82(0.39-1.73,P<0.0001) for rate of resection,1.53(1.33-2.84,P=0.007) for rate of complete resection,1.78(1.14-2.78,P=0.79) for operative mortality,1.12(0.89-2.48,P=0.503) for all treatment mortality,1.33(0.94-1.88,P=0.04) for the rate of adverse treatment,1.38(1.23-1.63,P=0.0002) for local-regional cancer recurrence,1.28(0.85-1.58,P=0.60) for distant cancer recurrence,and 1.27(0.86-1.65,P=0.19) for all cancer recurrence.A complete pathological response to chemoradiotherapy occurred in 10%-45.5% of patients.The 5-year survival benefi t was most pronounced when chemotherapy and radiotherapy were given concurrently(OR:1.45,95% CI:1.26-1.79,P=0.015) instead of sequentially(OR:0.85,95% CI:0.64-1.35,P=0.26).CONCLUSION:Compared with surgery alone,neoadjuvant chemoradiotherapy can improve the long-term survival and reduce local-regional cancer recurrence.Concurrent administration of neoadjuvant chemoradiotherapy was superior to sequential chemoradiotherapy. | Jin Lv Xiu-Feng Cao Bin Zhu Lv Ji Lei Tao Dong-Dong Wang | 2009 | World Journal of Gastroenterology2009，15，39： | 27 |
| 20 | Effect of nutrient level on phytoplankton community structure in different water bodies显示文摘Increasing levels of pollution within water bodies can cause eutrophication and an associated rapid growth in and reproduction of phytoplankton. Although most frequently occurring in bodies of water such as lakes and dams, in recent years an increasing number of river systems in China have suffered serious algal blooms. The community structure of phytoplankton may differ, however, dependent on the hydrodynamic conditions and nutrient levels within the water body. The field investigation results obtained from a stagnant river in Suzhou City and Taihu Lake, China, showed that in water with higher concentrations of nitrogen and phosphorus, Chlorophyta became the predominant species and in water with lower concentrations of nitrogen and phosphorus, Cyanobacteria became the predominant species. Growth experiments with competitive species, Microcystis aeruginosa Kutz and Scenedesmus quadricauda (Turp.), were conducted at three different nutrient levels. The biomass of algae in pure and mixed cultures was measured under conditions of different N/P ratios at oligotrophic, eutrophic and hypertrophic nutrient levels. The results indicated that the most suitable state for the growth and reproduction of M. aeruginosa and S. quadricauda were eutrophic conditions in both pure and mixed cultures. Under competition, however, the lower medium nutrient levels favoured M. aeruginosa, while the higher medium nutrient levels better suited S. quadricauda. Under similar hydrodynamic conditions, the community structure of phytoplankton in the water body was determined by the dominant species in competition for nutrients. | Wei Zhu Lei Wan Lianfang Zhao | 2010 | Journal of Environmental Sciences2010，22，1： | 25 |
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