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| 1 | CRISPR/Cas9-mediated gene editing in human tripronuclear zygotes显示文摘染色体编辑工具例如定期聚类短 palindromic 重复(CRISPR ) 联系了的 interspaced 系统(Cas ) 广泛地被用来包括动物接合子和人的房间在模型系统修改基因,并且为基本研究和临床的应用保持巨大的诺言。迄今为止,严肃的知识差距在人的早胚胎,并且在效率和在人的培植前胚胎使用象 CRISPR/Cas9 那样的技术的潜在的离开目标效果留在我们 DNA 修理机制的理解。在这份报告,我们使用了推进的接合子调查 CRISPR/Cas9-mediated 基因在人的房间编辑的 tripronuclear (3PN ) 。我们发现 CRISPR/Cas9 能有效地劈开内长的 -globin 基因(HBB ) 。然而,相应再结合的效率指导了 HBB 的修理(HDR ) 是低的,编辑胚胎是马赛克。离开目标劈开在由 T7E1 试金并且 whole-exome 定序揭示了的这些 3PN 接合子也是明显的。而且,内长的 delta-globin 基因(HBD ) 对 HBB 相应,与外长的施主 oligos 竞争了充当修理模板,导致倔强的变化。我们的数据也显示在这些胚胎的 HBB 地点的修理通过非转线路 HDR 小径优先地发生了。一起拿,我们的工作加亮紧迫的需要进一步改进 CRISPR/Cas9 平台的忠实和特性,为编辑的 CRSIPR/Cas9-mediated 的任何临床的应用程序的一个前提。 | Puping Liang Yanwen Xu Xiya Zhang Chenhui Ding Rui Huang Zhen Zhang Jie Lv Xiaowei Xie Yuxi Chen Yujing Li Ying Sun Yaofu Bai Zhou Songyang Wenbin Ma Canquan Zhou Junjiu Huang | 2015 | Protein & Cell2015，6，5： | 149 |
| 2 | Correction of β-thalassemia mutant by base editor in human embryos显示文摘 | Puping Liang Chenhui Ding Hongwei Sun Xiaowei Xie Yanwen Xu Xiya Zhang Ying Sun Yuanyan Xiong Wenbin Ma Yongxiang Liu Yali Wang Jianpei Fang Dan Liu Zhou Songyang Canquan Zhou Junjiu Huang | 2017 | Protein & Cell2017，8，11： | 34 |
| 3 | Effective gene editing by high-fidelity base editor 2 in mouse zygotes显示文摘通过相应再结合的指向的点 mutagenesis 广泛地在基因研究被使用了并且为在病人修理引起疾病的变化保持可观的诺言。然而,象 mosaicism 那样的问题和低 mutagenesis 效率继续提出挑战到如此的途径的临床的申请。最近,一个基础编辑器() 在 cytidine (C) 脱氨基酶上造的系统和 CRISPR/Cas9 技术在植物,酵母,和人的房间为指向的点 mutagenesis 作为一个其他的方法被开发。然而,基础编辑器高效地在 deamination 窗口中把 C 变换成 thymidine (T) 是否指向了在鼠标胚胎的基础编辑,仍然保持不清楚是可行的。在这份报告,我们产生了基础编辑的一个修改高保真版本 2 (HF2-BE2 ) ,并且调查了它在老鼠胚胎编辑功效的底。我们发现 HF2-BE2 能高效地把 C 变换成 T,与直到在老鼠胚胎的 100% biallelic 变化效率。不同于 BE3, HF2-BE2 能在目标和非目标海滨上把 C 变换成 T,扩展基础编辑器的编辑范围。令人惊讶地,我们发现 HF2-BE2 能也使脱去氨基对 gRNA 有约束力的区域近似的 C。一起拿,我们的工作表明由基础编辑,和下划线在鼠标产生点变化的可行性小心地优化编辑系统以便消除近似地点的 deamination 的底的需要。 | Puping Liang Hongwei Sun Ying Sun Xiya Zhang Xiaowei Xie Jinran Zhang zhen Zhang Yuxi Chen Chenhui Ding Yuanyan Xiong Wenbin Ma Dan Liu Junjiu Huang Zhou Songyang | 2017 | Protein & Cell2017，8，8： | 17 |
| 4 | New Technique of Casting-rolling Strips for Semi-solid Magnesium Alloys显示文摘The conjugation of semi-solid process technique and casting-rolling technique applied to produce the magnesium stripswas studied. The semi-solid slurry has been prepared continuously by the mechanical method and its temperaturewas controlled strictly at the same time. AZ91D and AZ31 casting magnesium alloys were applied to the experiment.The casting-rolling strips with non-dendritic structure were obtained and its main mechanical property is better. Theprocess ability of the casting-rolling strips was studied. It is significative to link the semi-solid process techniques andcasting-rolling techniques, through which we can get high quality magnesium alloy strips with non-dendritic structureand improve the overall properties of the products. | Shuisheng XIE Maopeng GENG Xinmin ZHOU Ying ZHANG Songyang ZHANG Yanchun WANG Guojie HUANG | 2005 | Journal of Materials Science & Technology2005，21，6： | 9 |
| 5 | Phosphorylation of PLIN3 by AMPK promotes dispersion of lipid droplets during starvation显示文摘Dear Editor,Lipid droplets(LDs)are dynamic lipid-storage organelles of storage depots and sources of essential substrates for myriad cellular processes and protect cells from lipotoxicity(Ohsaki et al.,2006).Disrupted LD and fat storage homeostasis has been linked to metabolic diseases such as atherosclerosis,obesity,and type II diabetes(Levin et al.,2001).Structurally,the core of neutral lipids in LDs is surroun ded by a phospholipid mono layer and coated with specific proteins(Storey et al.,2011).Perilipin family of proteins are the predominant LD-associated proteins. | Jianxi Zhu Mingyang Xu Yi Liu Lisha Zhuang Kejun Ying Feng Liu Dan Liu Wenbin Ma Zhou Songyang | 2019 | Protein & Cell2019，10，5： | 7 |
| 6 | Human telomeric proteins occupy selective interstitial sites显示文摘人的 telomeres 被在六核心 telomeric 蛋白质 RAP1, TRF1, TRF2, TIN2, TPP1,和 POT1 附近装配的蛋白质建筑群绑并且保护。telomeres 上的这些蛋白质的功能广泛地被学习了。最近,增加证据在 telomeres 外面为这些蛋白质建议了可能的角色。然而,不在经典中(extra-telomeric ) 人的 telomeric 蛋白质遗体的功能糟糕理解。到这个目的,我们系统地沿着人的染色体调查了 telomeric 蛋白质的有约束力的地点,由执行为 RAP1 和 TRF2 的整个染色体的染色质 immunoprecipitation (薄片) 。(ChIP-seq ) 薄片定序表明 RAP1 和 TRF2 能在空隙的地点的一个小数字上被发现,包括对基因近似的区域。一些这些有约束力的地点包含短 telomere 重复,建议蛋白质能直接绑在空隙的地点的那 telomeric。有趣地,仅仅可得到的空隙的 telomere 的小部分包含重复的区域被 RAP1 和 TRF2 占据。宫外地表示的 TRF2 能占据另外的空隙的 telomere 重复地点,建议蛋白质集中可以支配到空隙的地点的 telomeric 蛋白质的选择指向。由 RNA 干扰减少 RAP1 和 TRF2 表示导致了指向 RAP1 、指向 TRF2 的基因的改变的抄写。我们的结果显示人的 telomeric 蛋白质能占据一小部分空隙的地点并且调整基因抄写。 | Dong Yang Yuanyan Xiong Hyeung Kim Quanyuan He Yumei Li Rui Chen Zhou Songyang | 2011 | Cell Research2011，21，7： | 7 |
| 7 | Questions about NgAgo显示文摘 | Shawn Burgess Linzhao Cheng Feng Gu Zhiwei Huang Shuo Lin Jinsong Li Wei Li Wei Qin Yujie Sun Zhou Songyang Wensheng Wei Qiang Wu Haoyi Wang Xiaoqun Wang Jing-Wei Xiong Jianzhong Xi Hui Yang Bin Zhou Bo Zhang Junjiu Huang | 2016 | Protein & Cell2016，7，12： | 5 |
| 8 | Effective and precise adenine base editing n mouse zygotes显示文摘 | Puping Liang Hongwei Sun Xiya Zhang Xiaowei Xie Jinran Zhang Yaofu Bai Xueling Ouyang Shengyao Zhi Yuanyan Xiong Wenbin Ma Dan Liu Junjiu Huang Zhou Songyang | 2018 | Protein & Cell2018，9，9： | 4 |
| 9 | Live cell imaging and proteomic profiling of endogenous NEAT1 lncRNA by CRISPR/ Cas9-mediated knock-in显示文摘In mammalian cells,long noncoding RNAs(lncRNAs)form complexes with proteins to execute various biological functions such as gene transcription,RNA processing and other signaling activities.However,methods to track endogenous lncRNA dynamics in live cells and screen for lncRNA interacting proteins are limited.Here,we report the development of CERTIS(CRISPR-mediated Endogenous lncRNA Tracking and Immunoprecipitation System)to visualize and isolate endogenous lncRNA,by precisely inserting a 24-repeat MS2 tag into the distal end of lncRNA locus through the CRISPR7Cas9 technology.In this study,we show that CERTIS effectively labeled the paraspeckle lncRNA NEAT1 without disturbing its physiological properties and could monitor the endogenous expression variation of NEAT1.In addition,CERTIS displayed superior performance on both short-and long-term tracking of NEAT1 dynamics in live cells.We found that NEAT1 and paraspeckles were sensitive to topoisomerase I specific inhibitors.Moreover,RNA Immunoprecipitation(RIP)of the MS2-tagged NEAT1 lncRNA successfully revealed several new protein components of paraspeckle.Our results support CERTIS as a tool suitable to track both spatial and temporal lncRNA regulation in live cells as well as study the lncRNA-protein interactomes. | Bohong Chen Shengcheng Deng Tianyu Ge Miaoman Ye Jianping Yu Song Lin Wenbin Ma Zhou Songyang | 2020 | Protein & Cell2020，11，9： | 4 |
| 10 | Telomere regulation in pluripotent stem cells显示文摘Pluripotent 干细胞(PSC ) 有潜力从所有三基本细菌层和能力生产房间的任何类型到自我更新并且在 vitro 无止境地增殖。PSC,胚胎的干细胞(转换字符) 和导致的 pluripotent 干细胞(iPSCs ) 的二种主要类型,分享象殖民地形态学, Oct4 和 Nanog 的高表示,和强壮的碱的磷酸酶活动那样的普通特征。在最近的年里,增加证据建议那 telomere 长度在维持干细胞 pluripotency 代表另一个重要内部因素。Telomere 长度动态平衡和它的结构的正直帮助保护染色体结束免受再结合,结束熔化,和 DNA 的伤害损坏回答,保证哺乳动物的房间的师的能力。PSC 通常展出高 telomerase 活动坚持说他们的极其长、稳定的 telomeres,和新兴的数据显示小径可以玩的 telomeres (中高音) 的其他的变长在 telomere 的一个重要角色也工作。如此的特征对他们在 vivo 区分进多样的房间类型的能力多半关键。在这评论,我们将在转换字符和 iPSCs 集中于 telomeres 的功能和规定,从而使 telomere 长度的重要性清楚些到在 PSC 调整 telomeres 的 pluripotency 和机制。 | Yan Huang Puping Liang Dan Liu Junjiu Huang Zhou Songyang | 2014 | Protein & Cell2014，5，3： | 3 |
| 11 | Assessing the impact of urbanization on net primary productivity using multi-scale remote sensing data: a case study of Xuzhou, China显示文摘一条改进 Carnegie Ames 斯坦福途径(房屋)基于二种遥感( RS )为数据建模, Landsat 提高了题目的 Mapper 加(ETM +)并且中等分辨率成像分光辐射函数( MODIS ),并且气候变量被使用从 2001~2010 在每年的6月估计 Xuzhou 的网络主要生产率( NPP ).当空间规模在显示出的 Xuzhou 增加了陆上的植被的平均 NPP ,学习区域的 NPP 减少了最近的年里的一个减少的趋势,多半,由于在气候和环境的变化,学习区域被划分成四个分区,是指明了在在 NPP 指定为最低分区的区域与膨胀增加了的 NPP 最高、中等高、中等低、最低的规模,显示 NPP 分发与不同植被的 NPP 打的不同空间规模变化了被规模显著地也特别地影响,因为混合象素,生产的城市的树林的 NPP 降低估计在 NPP 的类似的趋势另外与不同 RS 数据被观察,生长区域的居住区域和减小的扩大是主要原因因为在城市的区域的 NPP 变化陆地盖子变化减少了 NPP ,它能主要被归因于导致人的骚乱。 | Kun TAN Songyang ZHOU Erzhu LI Peijun DU | 2015 | Frontiers of Earth Science2015，9，2： | 2 |
| 12 | Biomarkers of aging显示文摘Aging biomarkers are a combination of biological parameters to(i)assess age-related changes,(ii)track the physiological aging process,and(iii)predict the transition into a pathological status.Although a broad spectrum of aging biomarkers has been developed,their potential uses and limitations remain poorly characterized.An immediate goal of biomarkers is to help us answer the following three fundamental questions in aging research:How old are we?Why do we get old?And how can we age slower?This review aims to address this need.Here,we summarize our current knowledge of biomarkers developed for cellular,organ,and organismal levels of aging,comprising six pillars:physiological characteristics,medical imaging,histological features,cellular alterations,molecular changes,and secretory factors.To fulfill all these requisites,we propose that aging biomarkers should qualify for being specific,systemic,and clinically relevant. | Aging Biomarker Consortium Hainan Bao Jiani Cao Mengting Chen Min Chen Wei Chen Xiao Chen Yanhao Chen Yu Chen Yutian Chen Zhiyang Chen Jagadish K Chhetri Yingjie Ding Junlin Feng Jun Guo Mengmeng Guo Chuting He Yujuan Jia Haiping Jiang Ying Jing Dingfeng Li Jiaming Li Jingyi Li Qinhao Liang Rui Liang Feng Liu Xiaoqian Liu Zuojun Liu Oscar Junhong Luo Jianwei Lv Jingyi Ma Kehang Mao Jiawei Nie Xinhua Qiao Xinpei Sun Xiaoqiang Tang Jianfang Wang Qiaoran Wang Siyuan Wang Xuan Wang Yaning Wang Yuhan Wang Rimo Wu Kai Xia Fu-Hui Xiao Lingyan Xu Yingying Xu Haoteng Yan Liang Yang Ruici Yang Yuanxin Yang Yilin Ying Le Zhang Weiwei Zhang Wenwan Zhang Xing Zhang Zhuo Zhang Min Zhou Rui Zhou Qingchen Zhu Zhengmao Zhu Feng Cao Zhongwei Cao Piu Chan Chang Chen Guobing Chen Hou-Zao Chen Jun Chen Weimin Ci Bi-Sen Ding Qiurong Ding Feng Gao Jing-Dong JHan Kai Huang Zhenyu Ju Qing-Peng Kong Ji Li Jian Li Xin Li Baohua Liu Feng Liu Lin Liu Qiang Liu Qiang Liu Xingguo Liu Yong Liu Xianghang Luo Shuai Ma Xinran Ma Zhiyong Mao Jing Nie Yaojin Peng Jing Qu Jie Ren Ruibao Ren Moshi Song Zhou Songyang Yi Eve Sun Yu Sun Mei Tian Shusen Wang Si Wang Xia Wang Xiaoning Wang Yan-Jiang Wang Yunfang Wang Catherine CL Wong Andy Peng Xiang Yichuan Xiao Zhengwei Xie Daichao Xu Jing Ye Rui Yue Cuntai Zhang Hongbo Zhang Liang Zhang Weiqi Zhang Yong Zhang Yun-Wu Zhang Zhuohua Zhang Tongbiao Zhao Yuzheng Zhao Dahai Zhu Weiguo Zou Gang Pei Guang-Hui Liu | 2023 | Science China(Life Sciences)2023，66，5： | 2 |
| 13 | TPP1 as a versatile player at the ends of chromosomes显示文摘Telomeres,线性真核细胞的染色体的结束,是双人脚踏车 DNA 重复并且由各种各样的 telomeric 蛋白质盖住。这些核蛋白建筑群保护 telomeres 免受 DNA 的伤害损坏反应(DDR ) ,再结合,和端对端的熔化,保证染色体稳定性。人的 telosome/shelterin 建筑群是学习最好的联系 telomere 的蛋白质建筑群之一,由六核心 telomeric 蛋白质 TRF1, TRF2, TIN2, RAP1, POT1,和 TPP1 组成。TPP1,也已知的同样肾上腺皮质的发育异常蛋白质相当或相同的事物(ACD ) ,是 TEBP- 的一个通常认为的哺乳动物的相当或相同的事物并且属于 oligonucleotide 绑定(OB )-fold-containing 蛋白质家庭。三功能的域在 TPP1, N 终端 OB 褶层, POT1 有约束力的招募域(RD ) ,和 carboxyl 终端交往 TIN2 域(TID ) 以内被识别了。TPP1 能与 POT1 和 TIN2 交往维持 telomere 结构,并且调停为 telomere 延伸的 telomerase 招募。这些特征显示了 TPP1 玩在 telomere 的一个必要角色维护。这里,我们将考察重要调查结果加亮 TPP1 的功能的意义,与它和另外的 telosome 部件和 telomerase 的相互作用的一个焦点。我们将也在疾病治疗讨论潜在的含意。 | Sijie ZHANG | 2014 | Frontiers in Biology2014，9，3： | 1 |
| 14 | NLRX1 Negatively Regulates TLR-Induced NF-κB Signaling by Targeting TRAF6 and IKK显示文摘 | Xiaojun Xia Jun Cui Helen Y. Wang Liang Zhu Satoko Matsueda Qinfu Wang Xiaoang Yang Jun Hong Zhou Songyang Zhijian J. Chen Rong-Fu Wang | 2011 | Immunity2011，，6： | 1 |
| 15 | Structure of the SOCS4-ElonginB/C Complex Reveals a Distinct SOCS Box Interface and the Molecular Basis for SOCS-Dependent EGFR Degradation显示文摘 | Alex N. Bullock Maria C. Rodriguez Judit é. Debreczeni Zhou Songyang Stefan Knapp | 2007 | Structure2007，，11： | 1 |
| 16 | A Wnt- and beta-catenin-dependent pathway for mammalian cardiac myogenesis 显示文摘 | Nakamura T Sano M Zhou Songyang | 2003 | Proc Natl Aead Sei USA2003，100，10： | 1 |
| 17 | Whole-genome screening identifies proteins localized to distinct nuclear bodies显示文摘 | Ka-wing Fong Yujing Li Wenqi Wang Wenbin Ma Kunpeng Li Robert Z. Qi Dan Liu Zhou Songyang Junjie Chen | 2013 | The Journal of Cell Biology2013，，1： | 1 |
| 18 | A Wnt-and -catenin-dependent pathway for mammalian cardiac myogenesis显示文摘 | Teruya Nakamura Motoaki Sano Zhou Songyang | 2003 | PNAS2003，100，10： | 1 |
| 19 | Bend family proteins mark chromatin boundaries and synergistically promote early germ cell differentiation显示文摘Understanding the regulatory networks for germ cell fate specification is necessary to developing strategies for improving the efficiency of germ cell production in vitro.In this study,we developed a coupled screening strategy that took advantage of an arrayed bi-molecular fluorescence complementation(BiFC)platform for protein-protein interaction screens and epiblast-like cell(EpiLC)-induction assays using reporter mouse embryonic stem cells(mESCs).Investigation of candidate interaction partners of core human pluripotent factors OCT4,NANOG,KLF4 and SOX2 in EpiLC differentiation assays identified novel primordial germ cell(PGC)-inducing factors including BEN-domain(BEND/Bend)family members.Through RNA-seq,ChIP-seq,and ATAC-seq analyses,we showed that Bend5 worked together with Bend4 and helped mark chromatin boundaries to promote EpiLC induction in vitro.Our findings suggest that BEND/Bend proteins represent a new family of transcriptional modulators and chromatin boundary factors that participate in gene expression regulation during early germline development. | Guang Shi Yaofu Bai Xiya Zhang Junfeng Su Junjie Pang Quanyuan He Pengguihang Zeng Junjun Ding Yuanyan Xiong Jingran Zhang Jingwen Wang Dan Liu Wenbin Ma Junjiu Huang Zhou Songyang | 2022 | Protein & Cell2022，13，10： | 1 |
| 20 | SH2 domains recognize specific phosphopeptide sequences显示文摘 | ZHOU SONGYANG STEVEN E SHOELSON MANAS CHAUDHURI | 1993 | Cell1993，72，： | 1 |
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