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| 1 | Global Dynamic Molecular Profiling of Stomatal Lineage Cell Development by Single-Cell RNA Sequencing显示文摘The regulation of stomatal lineage cell development has been extensively investigated.However,a comprehensive characterization of this biological process based on single-cell transcriptome analysis has not yet been reported.In this study,we performed RNA sequencing on 12844 individual cells from the cotyledons of 5-day-old Arabidopsis seedlings.We identified 11 cell clusters corresponding mostly to cells at specific stomatal developmental stages using a series of marker genes.Comparative analysis of genes with the highest variable expression among these cell clusters revealed transcriptional networks that regulate development from meristemoid mother cells to guard mother cells.Examination of the developmental dynamics of marker genes via pseudo-time analysis revealed potential interactions between these genes.Collectively,our study opens the door for understanding how the identified novel marker genes participate in the regulation of stomatal lineage cell development. | Zhixin Liu Yaping Zhou Jinggong Guo Jiaoai Li Zixia Tian Zhinan Zhu Jiajing Wang Rui Wu Bo Zhang Yongjian Hu Yijing Sun Yan Shangguan Weiqiang Li Tao Li Yunhe Hu Chenxi Guo Jean-David Rochaix Yuchen Miao Xuwu Sun | 2020 | Molecular Plant2020，13，8： | 11 |
| 2 | A critical epitope in CD147 facilitates memory CD4^(+) T-cell hyper-activation in rheumatoid arthritis显示文摘The abnormal activation of CD4^(+)CD45RO+memory T(Tm)cells plays an important role in the pathogenesis of rheumatoid arthritis(RA).Previous studies have shown that CD147 participates in T-cell activation.However,it remains unclear whether CD147 is involved in abnormal Tm-cell activation in RA patients.In this study,we demonstrated that CD147 was predominantly upregulated in Tm cells derived from RA patients.The anti-CD147 mAb 5A12 specifically inhibited Tm-cell activation and proliferation and further restrained osteoclastogenesis.Using a structural–functional approach,we depicted the interface between 5A12 and CD147.This allowed us to identify two critical residues,Lys63 and Asp65,as potential targets for RA treatment,as the double mutation K63A/D65A inhibited Tm-cell activation,mimicking the neutralization by 5A12.This study provides not only a theoretical basis for a“CD147-Tm/Osteoclast-RA chain”for the potential prevention and treatment of RA or other T-cell-mediated autoimmune diseases but also a new target for related drug design and development. | Na Guo Sheng Ye Kui Zhang Xiaoling Yu Hongyong Cui Xiangmin Yang Peng lin Minghua Lv Jinlin Miao Yang Zhang Qing Han Rongguang Zhang Zhinan Chen Ping Zhu | 2019 | Cellular & Molecular Immunology2019，16，6： | 6 |
| 3 | Biodetoxifi cation of toxins generated from lignocellulose pretrcatment u sing a newly isolated fungus, Amorphotheca resinae ZN1, and the consequent ethanol fermentation 显示文摘 | Zhang lian Zhu Zhinan Wang Xiaofeng etal | 2010 | Biotechnology for Biofuels2010，3，： | 1 |
| 4 | Biodetoxification of toxins generated from lignocellulose pretreatment using a newly isolated fungus, Amorphotheca resinae ZNI, and the consequent ethanol fermentation显示文摘 | Jian Zhang Zhinan Zhu Xiaofeng Wang | 2010 | Biotechnology for Biofuels2010，3，： | 1 |
| 5 | Biodetoxification of toxins generated from lignoeellulose pretreatment using a newly isolated fungus,Amorptmtheca resinae ZN1,and the consequent ethanol fermentation 显示文摘 | ZHANG Jian ZHU Zhinan WANG Xiaofeng | 2010 | Biotechnology for Biofuels2010，26，3： | 1 |
| 6 | Biodetoxification of toxins generated from lignoceltulose pretreatment using a newly isolated fungus Amorphotheca resinae ZN1 and the consequent ethanol fermentation显示文摘 | Zhang Jian Zhu Zhinan Wang Xiaofeng | 2010 | Biotechnology for Biofuels2010，3，： | 1 |
| 7 | Biodetoxification of toxins generated from lignocellulose pretreatment using a newly isolated fungus, amorphotheca resinae ZN1, and the consequent ethanol fermentation显示文摘 | Zhang J ian Zhu Zhinan Wang Xiaofeng | 2010 | Biotechnology for Biofu- els2010，26，3： | 1 |
| 8 | Generation of high-yield rapamycin-producing strains through protoplasts-related techniques显示文摘 | Xiyang Chen Peilian Wei Limei Fan Dong Yang Xiangchen Zhu Wenhe Shen Zhinan Xu Peilin Cen | 2009 | Applied Microbiology and Biotechnology2009，，3： | 1 |
| 9 | Biodetoxifi- cation of toxins generated from lignocellulose pretreatment using a newly isolated fungus, Amorphotheca resinae ZN1, and the consequent ethanol fermentation显示文摘 | Zhang Jian Zhu Zhinan Wang Xiaofeng | 2010 | Biotechnology for Biofuels2010，3，： | 1 |
| 10 | Efficient production of l -lactic acid from hydrolysate of Jerusalem artichoke with immobilized cells of Lactococcus lactis in fibrous bed bioreactors显示文摘 | Zhouming Shi Peilian Wei Xiangcheng Zhu Jin Cai Lei Huang Zhinan Xu | 2012 | Enzyme and Microbial Technology2012，，5： | 1 |
| 11 | Inhibition of MALT1 paracaspase activity improves lesion recovery following spinal cord injury显示文摘Spinal cord injury(SCI) is a devastating traumatic injury that causes persistent, severe motor and sensory dysfunction. Immune responses are involved in functional recovery after SCI. Mucosa-associated lymphoid tissue lymphoma translocation 1(MALT1) has been shown to regulate the survival and differentiation of immune cells and to play a critical role in many diseases, but its function in lesion recovery after SCI remains unclear. In this paper, we generated KI(knock in) mice with a point mutation(C472 G) in the active center of MALT1 and found that the KI mice exhibited improved functional recovery after SCI.Fewer macrophages were recruited to the injury site in KI mice and these macrophages differentiated into anti-inflammatory macrophages. Moreover, macrophages from KI mice exhibited reduced phosphorylation of p65, which in turn resulted in decreased SOCS3 expression and increased pSTAT6 levels.Similar results were obtained upon inhibition of MALT1 paracaspase with the small molecule inhibitor‘‘MI-2' or the more specific inhibitor ‘‘MLT-827'. In patients with SCI, peripheral blood mononuclear cells(PBMC) displayed increased MALT1 paracaspase. Human macrophages showed reduced proinflammatory and increased anti-inflammatory characteristics following the inhibition of MALT1 paracaspase. These findings suggest that inhibition of MALT1 paracaspase activity in the clinic may improve lesion recovery in subjects with SCI. | Hua Zhang Guodong Sun Xiaowei Li Zhen Fu Chengbin Guo Guangchao Cao Baocheng Wang Qian Wang Shuxian Yang Dehai Li Xichun Xia Peng Li Jing Zhu Wei Zhou Liangyan Zheng Jingxia Li Lei Zhang Jianlei Hao Libing Zhou Frederic Bornancin Zhizhong Li Zhinan Yin Yunfei Gao | 2019 | Science Bulletin2019，64，16： | 0 |
| 12 | Bioinspired Rotation Microneedles for Accurate Transdermal Positioning and Ultraminimal-Invasive Biomarker Detection with Mechanical Robustness显示文摘Microneedle permits transdermal biosensing and drug delivery with minor pain.However,accurate microneedle transdermal positioning with minimal skin deformation remains a significant technical challenge due to inhomogeneous skin topology and discontinuous force applied to the microneedle.Here,we introduce bioinspired rotation microneedles for in vivo accurate microneedle positioning as inspired by honeybees’stingers.We demonstrate the benefits of rotation microneedles in alleviating skin resistance through finite element analysis,full-thickness porcine validations,and mathematical derivations of microneedleskin interaction stress fields.The max penetration force was mitigated by up to 45.7%and the force attenuation rate increased to 2.73 times in the holding stage after penetration.A decrease in max skin deflection and a faster deformation recovery introduced by rotation microneedles implied a more precise penetration depth.Furthermore,we applied the rotation microneedles in psoriasis mice,a monogenic disorder animal model,for minimally invasive biological sample extraction and proinflammatory cytokine monitoring.An ultrasensitive detection method is realized by using only one microneedle to achieve cytokine mRNA level determination compared to commonly required biopsies or blood collection.Thus,rotation microneedles permit a simple,rapid,and ultraminimal-invasive method for subcutaneous trace biological sample acquisition and subsequent point-of-care diagnostics with minimal damage to both microneedles and skins. | Yilin Cai Shiyi Huang Zhinan Zhang Jiazheng Zhang Xingyue Zhu Xiaoxiang Chen Xianting Ding | 2022 | Research2022，，3： | 0 |
| 13 | Cathepsin D knockdown regulates biological behaviors of granulosa cells and affects litter size traits in goats显示文摘Cathepsin D(CTSD),the major lysosomal aspartic protease that is widely expressed in different tissues,potentially regulates the biological behaviors of various cells.Follicular granulosa cells are responsive to the increase of ovulation number,hence indirectly influencing litter size.However,the mechanism underlying the effect of CTSD on the behaviors of goat granulosa cells has not been fully elucidated.This study used immunohistochemistry to analyze CTSD localization in goat ovarian tissues.Moreover,western blotting was applied to examine the differential expression of CTSD in the ovarian tissues of monotocous and polytocous goats.Subsequently,the effects of CTSD knockdown on cell proliferation,apoptosis,cell cycle,and the expression of candidate genes of the prolific traits,including bone morphogenetic protein receptor IB(BMPR-IB),follicle-stimulating hormone(FSHR),and inhibinα(INHA),were determined in granulosa cells.Results showed that CTSD was expressed in corpus luteum,follicle,and granulosa cells.Notably,CTSD expression in the monotocous group was significantly higher than that in the polytocous group.In addition,CTSD knockdown could improve granulosa cell proliferation,inhibit cell apoptosis,and significantly elevate the expression of proliferating cell nuclear antigen(PCNA)and B cell lymphoma 2(Bcl-2),but it lowered the expression of Bcl-2-associated X(Bax)and caspase-3.Furthermore,CTSD knockdown significantly reduced the ratios of cells in the G0/G1 and G2/M phases but substantially increased the ratio of cells in the S phase.The expression levels of cyclin D2 and cyclin E were elevated followed by the obvious decline of cyclin A1 expression.However,the expression levels of BMPR-IB,FSHR,and INHA clearly increased as a result of CTSD knockdown.Hence,our findings demonstrate that CTSD is an important factor affecting the litter size trait in goats by regulating the granulosa cell proliferation,apoptosis,cell cycle,and the expression of candidate genes of the prolific trait. | Zhinan ZHOU Xiang CHEN Min ZHU Weiwei WANG Zheng AO Jiafu ZHAO Wen TANG Lei HONG | 2021 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2021，22，11： | 0 |
| 14 | The role of AFAP1-AS1 in mitotic catastrophe and metastasis of triple-negative breast cancer cells by activating the PLK1 signaling pathway显示文摘Triple-negative breast cancer(TNBC)is characterized by fast growth,high metastasis,high invasion,and a lack of therapeutic targets.Mitosis and metastasis of TNBC cells are two important biological behaviors in TNBC malignant progression.It is well known that the long noncoding RNA AFAP1-AS1 plays a crucial role in various tumors,but whether AFAP1-AS1 is involved in the mitosis of TNBC cells remains unknown.In this study,we investigated the functional mechanism of AFAP1-AS1 in targeting Polo-like Kinase 1(PLK1)activation and participating in mitosis of TNBC cells.We detected the expression of AFAP1-AS1 in the TNBC patient cohort and primary cells by in situ hybridization(ISH),northern blot,fluorescent in situ hybridization(FISH)and cell nucleus/cytoplasm RNA fraction isolation.High AFAP1-AS1 expression was negatively correlated with overall survival(OS),disease-free survival(DFS),metastasis-free survival(MFS)and recurrence-free survival(RFS)in TNBC patients.We explored the function of AFAP1-AS1 by transwell,apoptosis,immunofluorescence(IF)and patient-derived xenograft(PDX)models in vitro and in vivo.We found that AFAP1-AS1 promoted TNBC primary cell survival by inhibiting mitotic catastrophe and increased TNBC primary cell growth,migration and invasion.Mechanistically,AFAP1-AS1 activated phosphorylation of the mitosis-associated kinase PLK1 protein.Elevated levels of AFAP1-AS1 in TNBC primary cells increased PLK1 pathway downstream gene expression,such as CDC25C,CDK1,BUB1 and TTK.More importantly,AFAP1-AS1 increased lung metastases in a mouse metastasis model.Taken together,AFAP1-AS1 functions as an oncogene that activates the PLK1 signaling pathway.AFAP1-AS1 could be used as a potential prognostic marker and therapeutic target for TNBC. | SHUIZHONG CEN XIAOJIE PENG JIANWEN DENG HAIYUN JIN ZHINAN DENG XIAOHUA LIN DI ZHU MING JIN YANWEN ZHU PUSHENG ZHANG YUNFENG LUO HONGYAN HUANG | 2023 | Oncology Research2023，31，3： | 0 |
| 15 | A Serological Survey of Contagious Caprine Pleuropneumonia in Taizhou City,Jiangsu Province显示文摘[Objective] This study aimed to investigate the prevalence of contagious caprine pleuropneumonia in Taizhou City,Jiangsu Province. [Method] By using indirect hemagglutination assay kits for detection of antibodies against Mycoplasma capricolum subsp. capripneumoniae( Mccp),Mycoplasma mycoides subsp.capriclonum( Mmc) and Mycopalsam ovipneumonia( Movi),1 157 goat sera from Taizhou City of Jiangsu Province were detected. [Result] The average positive detection rate of Mccp and Mmc in infected goats was 51. 85% and 39. 81%,respectively; the average positive detection rate of Mccp and Mmc in non-infected goats was 10. 52% and 5. 84%,respectively,and the total positive detection rate of Mycoplasma infections was 16. 37%. [Conclusion]Mycoplasma infections occur commonly in Taizhou City. The prevention and control of Mycoplasma infections should be strengthened. | Xingang HU Yinyun HUANG Tingting XU Zhinan ZHU Yajun TIAN Yuliang XU | 2015 | Agricultural Biotechnology2015，4，6： | 0 |
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