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| 1 | CRISPR/Cas9-mediated gene editing in human tripronuclear zygotes显示文摘染色体编辑工具例如定期聚类短 palindromic 重复(CRISPR ) 联系了的 interspaced 系统(Cas ) 广泛地被用来包括动物接合子和人的房间在模型系统修改基因,并且为基本研究和临床的应用保持巨大的诺言。迄今为止,严肃的知识差距在人的早胚胎,并且在效率和在人的培植前胚胎使用象 CRISPR/Cas9 那样的技术的潜在的离开目标效果留在我们 DNA 修理机制的理解。在这份报告,我们使用了推进的接合子调查 CRISPR/Cas9-mediated 基因在人的房间编辑的 tripronuclear (3PN ) 。我们发现 CRISPR/Cas9 能有效地劈开内长的 -globin 基因(HBB ) 。然而,相应再结合的效率指导了 HBB 的修理(HDR ) 是低的,编辑胚胎是马赛克。离开目标劈开在由 T7E1 试金并且 whole-exome 定序揭示了的这些 3PN 接合子也是明显的。而且,内长的 delta-globin 基因(HBD ) 对 HBB 相应,与外长的施主 oligos 竞争了充当修理模板,导致倔强的变化。我们的数据也显示在这些胚胎的 HBB 地点的修理通过非转线路 HDR 小径优先地发生了。一起拿,我们的工作加亮紧迫的需要进一步改进 CRISPR/Cas9 平台的忠实和特性,为编辑的 CRSIPR/Cas9-mediated 的任何临床的应用程序的一个前提。 | Puping Liang Yanwen Xu Xiya Zhang Chenhui Ding Rui Huang Zhen Zhang Jie Lv Xiaowei Xie Yuxi Chen Yujing Li Ying Sun Yaofu Bai Zhou Songyang Wenbin Ma Canquan Zhou Junjiu Huang | 2015 | Protein & Cell2015，6，5： | 149 |
| 2 | Correction of β-thalassemia mutant by base editor in human embryos显示文摘 | Puping Liang Chenhui Ding Hongwei Sun Xiaowei Xie Yanwen Xu Xiya Zhang Ying Sun Yuanyan Xiong Wenbin Ma Yongxiang Liu Yali Wang Jianpei Fang Dan Liu Zhou Songyang Canquan Zhou Junjiu Huang | 2017 | Protein & Cell2017，8，11： | 34 |
| 3 | Effective gene editing by high-fidelity base editor 2 in mouse zygotes显示文摘通过相应再结合的指向的点 mutagenesis 广泛地在基因研究被使用了并且为在病人修理引起疾病的变化保持可观的诺言。然而,象 mosaicism 那样的问题和低 mutagenesis 效率继续提出挑战到如此的途径的临床的申请。最近,一个基础编辑器() 在 cytidine (C) 脱氨基酶上造的系统和 CRISPR/Cas9 技术在植物,酵母,和人的房间为指向的点 mutagenesis 作为一个其他的方法被开发。然而,基础编辑器高效地在 deamination 窗口中把 C 变换成 thymidine (T) 是否指向了在鼠标胚胎的基础编辑,仍然保持不清楚是可行的。在这份报告,我们产生了基础编辑的一个修改高保真版本 2 (HF2-BE2 ) ,并且调查了它在老鼠胚胎编辑功效的底。我们发现 HF2-BE2 能高效地把 C 变换成 T,与直到在老鼠胚胎的 100% biallelic 变化效率。不同于 BE3, HF2-BE2 能在目标和非目标海滨上把 C 变换成 T,扩展基础编辑器的编辑范围。令人惊讶地,我们发现 HF2-BE2 能也使脱去氨基对 gRNA 有约束力的区域近似的 C。一起拿,我们的工作表明由基础编辑,和下划线在鼠标产生点变化的可行性小心地优化编辑系统以便消除近似地点的 deamination 的底的需要。 | Puping Liang Hongwei Sun Ying Sun Xiya Zhang Xiaowei Xie Jinran Zhang zhen Zhang Yuxi Chen Chenhui Ding Yuanyan Xiong Wenbin Ma Dan Liu Junjiu Huang Zhou Songyang | 2017 | Protein & Cell2017，8，8： | 17 |
| 4 | A universal coherent source for quantum key distribution显示文摘We propose a concept of universal coherent source for quantum key distribution. The weak coherent pulse (WCP) and heralded single photon source (HSPS) are the most common photon sources for state-of-art quantum key distribution (QKD). However, there exists a prominent crossover between the maximal secure distance and the secure key generating rate in short and middle distance if one applies these two sources in a practical decoy state quantum key distribution. It is shown that by combining the heralded pair coherent state (HPCS) photon source and the practical decoy state method together, one can not only strengthen the maximal secure transmission distance, but also improve key generat-ing rate at short and medium distance. Moreover, the advancement in key generating is not confined in the particular protocol utilized and can be easily checked for both BB84 and SARG protocol. Finally, we clearly demonstrate how the HPCS-based decoy method works effectively and feasibly by proposing an efficient HPCS-based '1 signal+2 decoy' state method. | ZHANG ShengLi ZOU XuBo LI ChuanFeng JIN ChenHui GUO GuangCan | 2009 | Chinese Science Bulletin2009，54，11： | 15 |
| 5 | High-precision timing of the Quaternary standard samples with thermal ionization mass spectrometry (TIMS) U-series method显示文摘Two national stalagmite standard (GBW04412, GBW04413) and one international coral standard (RKM4) have been determined by using thermal ionization mass spectrometry (TIMS) method. The values of ( 234 U/ 238 U) act , ( 230 Th/ 234 U) act and age are all consistent in error range with the standard values obtained by using α spectrometry. | Peng Zicheng Wang Zhaorong Sun Weidong Ma Zhibang Xia Ming Zhang Chenhui Chen Wenji Zhang Zhonglu An Zhisheng | 1998 | Chinese Science Bulletin1998，43，4： | 14 |
| 6 | Progress in experimental study of aqueous lubrication显示文摘Aqueous lubrication is commonly regarded as friendly to the environment.We review current knowledge of the behaviors and mechanisms of aqueous lubrication.Major methods for investigating aqueous lubrication are addressed.We describe studies on both friction and film formation performance of various water-based lubricants,Additionally,we discuss possible underlying mechanisms of aqueous lubrication.Attention is also drawn to continuing topics of investigation and some unsolved problems. | MA LiRan ZHANG ChenHui LIU ShuHai | 2012 | Chinese Science Bulletin2012，57，17： | 14 |
| 7 | Investigations of the superlubricity of sapphire against ruby under phosphoric acid lubrication显示文摘In this study,we address the superlubricity behavior of sapphire against ruby(or sapphire against itself)under phosphoric acid solution lubrication.An ultra-low friction coefficient of 0.004 was obtained under a very high contact pressure,with a virgin contact pressure up to 2.57 GPa.Related experiments have indicated that the load,sliding speed,and humidity of the test environment can affect superlubricity to some degree,so we tested variations in these conditions.When superlubricity appears in this study a thin film is present,consisting of a hydrogen bond network of phosphoric acid and water molecules adsorbed on the two friction surfaces,which accounts for the ultra-low friction.Most significantly,the wear rate of the sapphire and ruby in the friction process is very slow and the superlubricity state is very stable,providing favorable conditions for future technological applications. | Jinjin LI Chenhui ZHANG Mingming DENG Jianbin LUO | 2014 | Friction2014，2，2： | 13 |
| 8 | Expression of Rab25 correlates with the invasion and metastasis of gastric cancer显示文摘The objective of this study was to determine the expression of the important vesicle trafficking- regulating factor Rab25 in human gastric cancer tissues, to analyze the correlation between Rab25 protein expression with gastric cancer occurrence and development, and to discuss the correlation of Rab25 protein expression with gastric cancer cell metastasis. The overall aim was to provide experimental evidence that can be used to design future biological treatments of human gastric cancer. Human gastric cancer tissue and the adjacent normal gastric tissue were surgically removed, and immunohistochemistry and Western blotting were used to detect Rab25 protein expression. The correlation between Rab25 protein expression with the development and pathological characteristics of gastric cancer was analyzed. Using RNAi, Rab25 expression was reduced in the gastric cancer cell line MGC80-3, and the changes in MGC80-3 cell invasiveness were then monitored. Immunohistochemistry showed that the Rab25 protein expression rates were 78.21% and 23.08% in gastric carcinoma and the adjacent normal gastric tissue, respectively. Immunohistochemistry and Western blot results showed that Rab25 protein expression in gastric cancer was significantly higher than in adjacent normal gastric tissues (P<0.01). Less differentiated gastric cancer cells had higher expression of Rab25 protein (P<0.01). Gastric carcinomas from patients with a late pathological stage (III-IV) had significantly higher Rab25 protein expression than early stage (I-II) patients (P<0.01). Gastric carcinomas from patients with lymph node metastasis had significantly higher Rab25 protein expression than lymph node metastasis- free patients (P<0.01). Gastric carcinomas from patients with distant metastases had significantly higher Rab25 protein expression than the distant metastasis-negative patients (P<0.01). Rab25 protein expression in gastric cancer was not affected by the patients , sex, age, or tumor size (P>0.05). MGC80-3 cells transfected with Rab25 siRNA had significantly lower Rab25 protein expression (P<0.01) and a significantly lower number of cells that passed through a Transwell chamber compared with non-transfected controls and the transfected control group (P<0.01). Rab25 protein expression is associated with the development of gastric cancer. siRNA knockdown of Rab25 protein expression in MGC80-3 gastric cancer cells reduced MGC80-3 cell invasiveness and provided experimental evidence for potential future biological treatment strategies of human gastric cancer. | Chuanwu Cao Chenhui Lu Jichong Xu Jiaxing Zhang Jun Zhang Maoquan Li | 2013 | Chinese Journal of Cancer Research2013，25，2： | 12 |
| 9 | Hydrodynamic effect on the superlubricity of phosphoric acid between ceramic and sapphire显示文摘In this work,a super-low friction coefficient of 0.003 was found between a silicon nitride ball and a sapphire plate lubricated by phosphoric acid solution.The wear mainly occurred in the running-in period and disappeared after superlubricity was achieved.The friction coefficient was effectively reduced from 0.3 to 0.003 at a constant speed of 0.076 m/s,accompanied by a 12-nm-thickness film.The lubrication regime was indicated to change from boundary lubrication in the running-in period to elastohydrodynamic lubrication in the superlubricity period,which is also supported by the results of the friction coefficient versus sliding speed.In addition,the experimental results showed good agreement with theoretical calculations based on the elastohydrodynamic lubrication theory,suggesting a significant hydrodynamic effect of phosphoric acid on superlubricity. | Mingming DENG Chenhui ZHANG Jinjin LI Liran MA Jianbin LUO | 2014 | Friction2014，2，2： | 11 |
| 10 | Modified graphene as novel lubricating additive with high dispersion stability in oil显示文摘Graphene is a promising material as a lubricant additive for reducing friction and wear.Here,a dispersing method which combines chemical modification of graphene by octadecylamine and dicyclohexylcarbodiimide with a kind of effective dispersant has been successfully developed to achieve the remarkable dispersion stability of graphene in base oil.The stable dispersion time of modified graphene(0.5 wt%)with dispersant(1 wt%)in PAO-6 could be up to about 120 days,which was the longest time reported so far.At the same time,the lubricant exhibits a significant improvement of tribological performance for a steel ball to plate tribo-system with a normal load of 2 N.The coefficient of friction between sliding surfaces was~0.10 and the depth of wear track on plate was~21 nm,which decreased by about 44%and 90%when compared to pure PAO-6,respectively.Furthermore,the analysis of the lubricating mechanisms in regard to the sliding-induced formation of nanostructured tribo-film has been contacted by using Raman spectra and TEM. | Pu WU Xinchun CHEN Chenhui ZHANG Jiping ZHANG Jianbin LUO Jiyang ZHANG | 2021 | Friction2021，9，1： | 9 |
| 11 | Practical security against linear cryptanalysis for SMS4-like ciphers with SP round function显示文摘SMS4,a block cipher whose global structure adopts a special unbalanced Feistel scheme with SP round function,is accepted as the Chinese National Standard for securing Wireless LANs.In this paper,in order to evaluate the security against linear cryptanalysis,we examine the upper bound of the maximum linear characteristic probability of SMS4-like ciphers with SP round function.In the same way as for SPN ciphers,it is sufficient to consider the lower bound of the number of linear active s-boxes.We propose a formula to compute the lower bound of the number of linear active s-boxes with regard to the number of rounds.The security threshold of SMS4-like ciphers can be estimated easily with our result.Furthermore,if the number of input words in each round of SMS4-like cipher is m,we find that it is unnecessary for designers to make the linear branch number of P greater than 2 m with respect to linear cryptanalysis. | ZHANG Bin JIN ChenHui | 2012 | Science China(Information Sciences)2012，55，9： | 6 |
| 12 | Flower-like NiCo_(2)S_(4)nanosheets with high electrochemical performance for sodium-ion batteries显示文摘A three-dimensional flower-like NiCo_(2)S_(4) formed by two-dimensional nanosheets is synthesized by a facile hydrothermal method and utilized as the anode for sodium-ion batteries.Studies have shown that materials can achieve the best performance under the ether-based electrolyte system with voltage ranging from 0.3 to 3 V,which could effectively avoid the dissolution of polysulfides and over-discharge of the material.Here,sodium storage mechanism and charge compensation behaviors of this ternary metal sulfide are comprehensively investigated by ex situ X-ray diffraction.Moreover,ex situ Raman spectra,ex situ X-ray photoelectron spectroscopy and transmission electron microscopy measurements are used to related tests for the first time.Additionally,quantitative kinetic analysis unravels that sodium storage partially depends on the pseudocapacitance mechanism,resulting in good specific capacity and excellent rate performance.The initial discharge capacity is as high as 748 mAh·g^(−1)at a current density of 0.1 A·g^(−1)with the initial coulomb efficiency of 94%,and the capacity can still maintain at 580 mAh·g^(−1)with the Coulomb efficiency close to 100%after following 50 cycles.Moreover,by the long cycle test at a high current density of 2 A·g^(−1),the capacity can still reach at 376 mAh·g^(−1)after over 500 cycles. | Yongqiang Miao Xiaosen Zhao Xin Wang Chenhui Ma Lu Cheng Gang Chen Huijuan Yue Lei Wang Dong Zhang | 2020 | Nano Research2020，13，11： | 5 |
| 13 | A β-glucosidase-producing M-2 strain: Isolation from cow dung and fermentation parameter optimization for flaxseed cake显示文摘Flaxseed cake contains cyanogenic glucosides, which can be metabolized into hydrocyanic acid in an animal's body, leading to asphyxia poisoning in cells. Beta-glucosidase is highly efficient in degrading cyanogenic glucosides. The Cattle may have b-glucosidase-producing strains in the intestinal tract after eating small amounts of flaxseed cake for a long time. This study aimed to isolate of a strain from cow dung that produces b-glucosidase with high activity and can significantly reduce the amount of cyanogenic glucosides. We used cow dung as the microflora source and an esculin agar as the selective medium. After screening with 0.05% esculin and 0.01% ferric citrate, we isolated 5 strains producing high amounts of b-glucosidase. In vitro flaxseed cake fermentation was fermented by these 5 strains, in which the strain M-2 exerted the best effect(P < 0.05). The strain M-2 was identified as Lichtheimia ramosa and used as the fermentation strain to optimize the fermentation parameters by a single factor analysis and orthogonal experimental design. The optimum condition was as follows: inoculum size3%, water content 60%, time 144 h, and temperature 32℃. Under this condition, the removal rate of cyanogenic glucosides reached 89%, and crude protein increment reached 44%. These results provided a theoretical basis for the removal of cyanogenic glucosides in flaxseed and the comprehensive utilization of flaxseed cake. | Chenhui Li Junshu Wei Yaping Jing Baoxia Teng Pingrong Yang Xinjun Chen Haiying Huang Tang Zhao Tuanjie Che Chunjiang Zhang | 2019 | Animal Nutrition2019，，1： | 5 |
| 14 | IL-17-triggered downregulation of miR-497 results in high HIF-1α expression and consequent IL-1β and IL-6 production by astrocytes in EAE mice显示文摘Interleukin 17(IL-17)is increasingly recognized as a key factor that contributes to the pathogenesis of multiple sclerosis(MS)and its experimental mouse autoimmune encephalomyelitis(EAE)model.However,the roles and regulatory mechanisms of IL-17-induced pro-inflammatory cytokine production in EAE mice remain largely unclear.In this study,the expression of IL-17,hypoxia inducible factor-1α(HIF-1α),IL-1β,IL-6 and microRNA-497(miR-497),as well as their intrinsic associations,was investigated using EAE model mice and cultured astrocytes exposed to IL-17 in vitro.We observed markedly increased production of IL-17,HIF-1α,IL-1βand IL-6 in the brain tissues of EAE mice,while the expression and secretion of HIF-1α,IL-1βand IL-6 were also significantly increased when cultured primary astrocytes from mice were stimulated with IL-17.Meanwhile,the expression of miR-497 was downregulated both in vivo and in vitro.Subsequent in vitro experiments revealed that IL-17 induced the production of IL-1βand IL-6 in astrocytes through the upregulation of HIF-1αas a transcriptional factor,indicating that IL-17-mediated downregulation of miR-497 enhanced HIF-1αexpression.Furthermore,astrocytespecific knockdown of IL-17RA and HIF-1αor astrocyte-specific overexpression of miR-497 by infection with different lentiviral vectors containing an astrocyte-specific promotor markedly decreased IL-1βand IL-6 production in brain tissues and alleviated the pathological changes and score of EAE mice.Collectively,these findings indicate that decreased miR-497 expression is responsible for IL-17-triggered high HIF-1αexpression and consequent IL-1βand IL-6 production by astrocytes in EAE mice. | Kai Shan Rongrong Pang Chenhui Zhao Xiaomei Liu Wenxing Gao Jing Zhang Dan Zhao Yingwei Wang Wen Qiu | 2017 | Cellular & Molecular Immunology2017，14，11： | 5 |
| 15 | Study of lubrication behavior of pure water for hydrophobic friction pair显示文摘The perfluorooctyltrichlorosilane molecular layer was self-assembled on glass plate. The tribological properties of the molecular layer in water were studied with the method of ball on disk. An interesting phenomenon was found that low friction coefficients of 0.02―0.08 were obtained when the friction pair was lubricated with only a water droplet. Whereas, when the friction pair was encircled with large amount of water or fully immersed in water, the friction coefficient was higher than that under a droplet lubrication. A mechanism of water droplet lubrication was proposed that the surface tension caused by the solid-liquid-air three-phase interface makes water molecules enter into the contact zone, which separates the two friction surfaces and provides a low friction coefficient. However, water film can hardly form when more water encircles the friction pair, due to the attraction between water molecules. | MA ZhiZuo ZHANG ChenHui LIU ShuHai LUO JianBin LU XinChun WEN ShiZhu | 2009 | Science China(Technological Sciences)2009，52，11： | 4 |
| 16 | Antrodia Cinnamomea ameliorates neointimal formation by inhibiting infl ammatory cell infi ltration through downregulation of adhesion molecule expression in vitro and in vivo显示文摘The increased vascular infl ammation is a key event in the development of atherosclerotic lesions.Antrodia cinnamomea has been shown to promote anticancerogenic activity through decreasing infl ammation.However,the potential role of A.cinnamomea in cardiovascular diseases remains unexplored.Herein,using carotid arterial ligation models,we found that ethanol extract from A.cinnamomea(EEAC)signifi cantly inhibited neointimal hyperplasia in a dose-dependent manner,accompanied with the reduced expression of activated p65 and infl ammatory cytokines.We also show that EEAC ameliorated TNF-α-induced phosphorylation of p65 and pro-infl ammatory cytokine expression in both vascular smooth muscle cells(VSMCs)and macrophages in vitro.Mechanistically,EEAC suppressed expression levels of intercellular adhesion molecule-1(ICAM-1)and vascular cell adhesion molecule(VCAM-1)in VSMCs,which attenuates the ability of monocytes/macrophages adhesion to VSMCs.Furthermore,the expression level of these adhesion molecules and infi ltration of monocytes/macrophages were also decreased in neointimal VSMCs of arteries pretreated with EEAC.Altogether,our results reveal a novel function of A.cinnamomea in suppressing vascular infl ammation upon ligation injury during neointimal formation,likely through inhibition of infl ammatory cell infi ltration via downregulating the adhesion molecules in VSMCs.Thus,A.cinnamomea may offer a pharmacological therapy to slow down disease progression in patients with vascular injury. | Yan Zhang Aijin Ma Hao Xi Ning Chen Rong Wang Chenhui Yang Jinbang Chen Pin Lü Fuping Zheng Wenyi Kang | 2021 | Food Science and Human Wellness2021，10，4： | 4 |
| 17 | Sn02 nanoparticles anchored on graphene oxide as advanced anode materials for high-performance lithium-ion batteries显示文摘Lithium-ion batteries (LIBs) with high energy density have attracted great attention for their wide applications in electric vehicles, and the exploration of the nextgeneration anode materials with high theoretical capacity is highly desired. In this work, Sn02 nanoparticles with the particle size of 200 nm uniformly anchored on the surface of graphene oxide (GO) was prepared by combination of the ultrasonic method and the following calcination process. The Sn02/GO composite with the weight ratio of Sn02 to GO at 4:1 exhibits excellent electrochemical performance, which originates from the synergistic effects between GO and Sn02 nanoparticles. A high discharge capacity of 492 mA·h·g^-1 can be obtained after 100 cycles at 0.2C, and after cycling at higher current densities of 1C and 2C, a discharge capacity of 641 mA·h·g^-1 can be restored when the current density goes back to 0.1C. The superior electrochemical performance and simple synthesis process make it a very promising candidate as anode materials for LIBs. | Ruiping LIU Ning ZHANG Xinyu WANG Chenhui YANG Hui CHENG Hanqing ZHAO | 2019 | Frontiers of Materials Science2019，13，2： | 4 |
| 18 | Superlubricity of Si_3N_4 sliding against SiO_2 under linear contact conditions in phosphoric acid solutions显示文摘Superlubricity phenomena, which were previously achieved merely under point contact conditions, have been observed under the linear contact condition between the silicon nitride (Si3N4) cylinder and the silica (SiO2) ring in phosphoric acid solutions.After a total 800 s running-in period and low friction stage, the friction coefficient finally declined to about 0.003. The ultralow friction coefficient could last for more than 5 h. In these experiments, a torque sensor of high accuracy was used to make sure that the measuring result was credible. The differences between superlubricity experiments in our group and others were dis-cussed. Unlike the situations in other superlubricity experiments about silicon nitride, where the specimens were immersed in water totally, the phosphoric acid solution with volume of only 150 μL was used in these tests. It was proved that free water in the solution had a great relationship to the necessary time of acquiring superlubricity and phosphoric acid played a unique role in the superlubricity, which could not be explained properly by the hydrodynamic lubrication model proposed by others. Fi-nally, based on the results of the friction tests and the analysis with scanning electron microscope (SEM), a modified hydro-gen-bonded network and hydrated shearing layer model was proposed to explain the mechanism of superlubricity. | SUN Liang ZHANG ChenHui LI JinJin LIU YuHong LUO JianBin | 2013 | Science China(Technological Sciences)2013，56，7： | 4 |
| 19 | Correlation between the mechanical properties and the<110>texture in a hot-rolled nearβtitanium alloy显示文摘1.Introduction Nearβtitanium alloys have been considered as a potential material to fabricate high-strength fasteners in aerospace field owing to their high specific strength,good crack and fatigue resistance[1,2].It is well known that the processing technology determines the microstructure,and the microstructure eventually correlates with the performance of alloys. | Ruifeng Dong Xiaoyang Zhang Chenhui Li Yuhong Zhao Jinzhong Tian Li Wu Hua Hou | 2022 | Journal of Materials Science & Technology2022，，2： | 4 |
| 20 | A novel rat model of cardiopulmonary bypass for deep hypothermic circulatory arrest without blood priming显示文摘 | Zhang Weihua Zhang Yanbo Liu Donghai Zhu Yaobin Qiao Chenhui Wang Jiaxiang Xu Yulin Liu Yang Li Bin Yang Yao | 2014 | Chinese Medical Journal2014，，7： | 3 |
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