|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Clinical Features of Adult/Adolescent Atopic Dermatitis and Chinese Criteria for Atopic Dermatitis显示文摘 | Ping Liu Yan Zhao Zhang-Lei Mu Qian-Jin Lu Qian-Jin L U Li Zhang Xu Yao Min Zheng Yi-Wen Tang Xin-Xiano Lu Xiu-Juan xia You-Kun Lin Yu-Zhen Li Cai-Xia Tu Zhi-Rong Yao Jin-Hua Xu Wei Li Wei Lai Hui-Min Yang Hong-Fu Xie Xiu-Ping Han Zhi-Qiang Xie Xiang Nong Zai-Pei Guo Dan-Qi Deng Tong-Xin Shi Jian-Zhong Zhang | 2016 | Chinese Medical Journal2016，，7： | 64 |
| 2 | Effect of Chaiqinchengqi decoction on sarco/endoplasmic reticulum Ca^(2+)-ATPase mRNA expression of pancreatic tissues in acute pancreatitis rats显示文摘AIM: To investigate the effect of Chaiqinchengqi decoction (CQCQD) on sarco/endoplasmic reticulum Ca2+-ATPase (SERCA) mRNA expression of pancreatic tissues in acute pancreatitis (AP) rats. METHODS: Thirty Sprague-Dawley (SD) rats were randomized into control group, AP group and CQCQD group (n = 3 × 10). The rats in the CQCQD group were intragastrically administered with CQCQD (10 mL/kg every 2 h) after induction of AP by intraperitoneal injection of caerulein (50 μg/kg.h × 5) within 4 h. At 6 h after the induction of AP model, pancreatic tissues were collected for the pathological observation, mRNA extraction for determination of SERCA1 and SERCA2 mRNA expression or pancreatic acinar cell isolation for measurement of fluorescence intensity (FI) of intracellular calcium ion concentration [Ca2+]i. RESULTS: There was no expression of pancreatic SERCA1 mRNA in the control group and the AP group. The expression of pancreatic SERCA2 mRNA in the AP group was down-regulated (expression ratio = 0.536; P = 0.001) compared with the control group, while that in the CQCQD group was up-regulated (expression ratio= 2.00; P = 0.012) compared with AP group. The FI of intracellular [Ca2+] of pancreatic acinar cells in the AP group (138.2 ± 23.1) was higher than the C group (111.0 ± 18.4) and the CQCQD group (118.7 ± 15.2 ) (P < 0.05) and the pancreatic pathological score in the CQCQD group was lower than that in the AP group (5.7 ± 1.9 vs 9.2 ± 2.7, P < 0.05).CONCLUSION: CQCQD can up-regulate the expression of SERCA2 mRNA of pancreatic tissues, reduce intracellular calcium overload and relieve pancreatic tissue lesions. | Ping Xue Li-Hui Deng Zhao-Da Zhang Xiao-Nan Yang Qing Xia Da-Kai Xiang Lei Huang Mei-Hua Wan | 2008 | World Journal of Gastroenterology2008，14，15： | 20 |
| 3 | Effect of endostar combined with cisplatin on expression of VEGF and Sema3A of Lewis lung cancer rats显示文摘Objective:To evaluate the therapeutic effect of endostar(ED) combined with cisplatin(DDP) on model of C57BL/6 rats,and to further investigate the inhibiting mechanism of endostar from tumor angiogenesis.Methods:Lewis lung cancer cells were inoculated in C57BL/6 mouse,then the mouse were randomized into control group(group A),ED(group B),DDP(group C) and ED/DDP (group D).They were treated according to the plan.And the expressions of VEGF and Sema3A were evaluated by immunhistochemisty.Results:The weight of tumor increased in group A and B.It was decreased in group C and D.The tumor volume was increased in all the 4 groups. The VEGF expression of group D was obviously lower than the other group 3,but the Sema3A expressed of group D was significantly strengthener than the other group 3.The VEGF expression of group B and group D were obviously low especially in the 4th-8th days.Pearson correlated analysis showed that the expression VEGF and Sema3A were negatively correlated(r=-0.72, P<0.05).Conclusions:ED combined with DDP could control the tumor growth effectively,and avoid weight loss.ED could reduce VEGF expression,and enhance Sema3A expression.Tumor vessel presents transient normalization.It is easy for DDP perfusion,and to kill tumor cells. | Ping Feng Zhi-Lin Zhang Zhi-Hua Zhang Xiu-Long Zhang Feng Xiang Jian-Hua Tang Bao-Li Xiang | 2013 | Asian Pacific Journal of Tropical Medicine2013，6，1： | 14 |
| 4 | Role of Fas/FasL Pathway-mediated Alveolar Macrophages Releasing Inflammatory Cytokines in Human Silicosis显示文摘The role of Fas/Fas ligand (FasL) in mediating alveolar macrophage (AM)-derived inflammatory cytokines in 29 human lung silicosis patients and 6 controls was studied.It showed that the serum levels of IL-1β,TNF-α,IL-8,MIP-1α,MCP-1,and TGF-β1were higher in silicosis patients than in controls,and increased with the progression of silicosis except TGF-β1 and IL-8.TGF-β1,IL-8,MCP-1,MIP-1αwere efficiently down-regulated by anti-FasL antibody or caspase-8 inhibitor,indicating that Fas/FasL signaling promotes the | YAO San Qiao HE Qin Cheng YUAN Ju Xiang CHEN Jie CHEN Gang LU Yao BAI Yu Ping ZHANG Chun Min YUAN Yang XU Ying Jun | 2013 | Biomedical and Environmental Sciences2013，26，11： | 13 |
| 5 | Solution structure of the RNA recognition domain of METTL3-METTL14 N^6-methyladenosine methyltransferase显示文摘N^6-methyladenosine(m6A),a ubiquitous RNA modification,is installed by METTL3-METTL14 complex.The structure of the heterodimeric complex between the methyltransferase domains(MTDs)of METTL3 and METTL14 has been previously determined.However,the MTDs alone possess no enzymatic activity.Here we present the solution structure for the zinc finger domain(ZFD)of METTL3,the inclusion of which fulfills the methyltransferase activity of METTL3-METTL14.We show that the ZFD specifically binds to an RNA containing 5'-GGACU-3'consensus sequence,but does not to one without.The ZFD thus serves as the target recognition domain,a structural feature previously shown for DNA methyltransferases,and cooperates with the MTDs of METTL3-METTL14 for catalysis.However,the interaction between the ZFD and the specific RNA is extremely weak,with the binding affinity at several hundred micromolar under physiological conditions.The ZFD contains two CCCH-type zinc fingers connected by an anti-parallel P-sheet.Mutational analysis and NMR titrations have mapped the functional interface to a contiguous surface.As a division of labor,the RNA-binding interface comprises basic residues from zinc finger 1 and hydrophobic residues fromβ-sheet and zinc finger 2.Further we show that the linker between the ZFD and MTD of METTL3 is flexible but partially folded,which may permit the cooperation between the two domains during catalysis.Together,the structural characterization of METTL3 ZFD paves the way to elucidate the atomic details of the entire process of RNA m6A modification. | Jinbo Huang Xu Dong Zhou Gong Ling-Yun Qin Shuai Yang Yue-Ling Zhu Xiang Wang Delin Zhang Tingting Zou Ping Yin Chun Tang | 2019 | Protein & Cell2019，10，4： | 11 |
| 6 | Effects of Chai-Qin-Cheng-Qi Decoction on cefotaxime in rats with acute necrotizing pancreatitis显示文摘AIM:To investigate the effect of Chai-Qin-Cheng-Qi Decoction(CQCQD)on cefotaxime(CTX)concentration in pancreas of rats with acute necrotizing pancreatitis (ANP). METHODS:Sixty healthy male Sprague-Dawley rats were divided randomly into an ANP group(ANP model +CTX,n=20),treatment group(ANP model+CTX +CQCQD,n=20)and control group(normal rats+ CTX,n=20).ANP models were induced by retrograde intraductal injection of 3.5%sodium taurocholate (1 mL/kg),and the control group was injected intraductally with normal saline.All rats were injected introperitoneally with 0.42 g/kg CTX(at 12-h intervals for a continuous 72 h)at 6 h after intraductal injection. Meanwhile,the treatment group received CQCQD (20 mL/kg)intragastrically at 8-h intervals,and the ANP and control group were treated intragastrically with normal saline.At 15 min after the last CTX injection,blood and pancreas samples were collected for the determination of CTX concentration using validated high-performance liquid chromatography. Pathological changes and wet-to-dry-weight(W/D) ratio of pancreatic tissue were examined. RESULTS:Serum CTX concentrations in three groups were not significantly different.Pancreatic CTXconcentration and penetration ratio were lower in ANP group vs control group(4.4±0.6μg/mL vs 18.6± 1.7μg/mL,P=0.000;5%vs 19%,P=0.000),but significantly higher in treatment group vs ANP group (6.4±1.7μg/mL vs 4.4±0.6μg/mL,P=0.020;7% vs 5%,P=0.048).The histological scores and W/D ratio were significantly decreased in treatment group vs ANP and control group. CONCLUSION:CQCQD might have a promotive effect on CTX concentration in pancreatic tissues of rats with ANP. | Li-Hui Deng Da-Kai Xiang Ping Xue Hai-Yan Zhang Lei Huang Qing Xia | 2009 | World Journal of Gastroenterology2009，15，35： | 9 |
| 7 | Association of a SLC30A8 Genetic Variant with Monotherapy of Repaglinide and Rosiglitazone Effect in Newly Diagnosed Type 2 Diabetes Patients in China显示文摘Objective To investigate a potential relationship between Solute carrier family 30 (zinc transporter) member 8 (SLC30A8) rs13266634 variant and efficacy of rosiglitazone or repaglinide in treating newly diagnosed Chinese type 2 diabetes patients.Methods A total of 209 diabetic patients without any antihyperglycemic history were recruited and treated with repaglinide or rosiglitazone randomly for 48 weeks (104 and 105 patients,respectively).Anthropometric measurements and clinical laboratory tests were carried out before and after the treatment.An non-synonymous variant rs13266634 was genotyped by matrix-assisted laser desorption ionization-time of flight mass spectroscopy.Results Ninety-one patients in repaglinide group and ninety-three patients in rosiglitazone group completed the study.Δ value of homeostasis model assessment of beta cell function (HOMA-B)and Δ value of fasting proinsulin levels were statistically significant between three genotype groups (P=0.0149 and 0.0246,respectively) after rosiglitazone treatment.However,no genotype association was observed in the repaglinide or rosiglitazone group with other parameters.Conclusion The SLC30A8 variant was associated with the efficacy of insulin sensitizer monotherapy on insulin secretion in patients with newly diagnosed type 2 diabetes mellitus in Shanghai,China. | JIANG Feng LI Qing HU Cheng ZHANG Rong WANG Cong Rong YU Wei Hui LU Jing Yi TANG Shan Shan BAO Yu Qian XIANG Kun San JIA Wei Ping | 2012 | Biomedical and Environmental Sciences2012，25，1： | 8 |
| 8 | Status and development of high-power laser facilities at the NLHPLP显示文摘In this paper, we review the status of the multifunctional experimental platform at the National Laboratory of High Power Laser and Physics(NLHPLP). The platform, including the SG-II laser facility, SG-II 9th beam, SG-II upgrade(SG-II UP) facility, and SG-II 5 PW facility, is operational and available for interested scientists studying inertial confinement fusion(ICF) and a broad range of high-energy-density physics. These facilities can provide important experimental capabilities by combining different pulse widths of nanosecond, picosecond, and femtosecond scales. In addition, the SG-II UP facility, consisting of a single petawatt system and an eight-beam nanosecond system, is introduced including several laser technologies that have been developed to ensure the performance of the facility. Recent developments of the SG-II 5 PW facility are also presented. | Jianqiang Zhu Jian Zhu Xuechun Li Baoqiang Zhu Weixin Ma Xingqiang Lu Wei Fan Zhigang Liu Shenlei Zhou Guang Xu Guowen Zhang Xinglong Xie Lin Yang Jiangfeng Wang Xiaoping Ouyang Li Wang Dawei Li Pengqian Yang Quantang Fan Mingying Sun Chong Liu Dean Liu Yanli Zhang Hua Tao Meizhi Sun Ping Zhu Bingyan Wang Zhaoyang Jiao Lei Ren Daizhong Liu Xiang Jiao Hongbiao Huang Zunqi Lin | 2018 | High Power Laser Science and Engineering2018，6，4： | 8 |
| 9 | Random dynamic analysis of vertical train–bridge systems under small probability by surrogate model and subset simulation with splitting显示文摘The response of the train–bridge system has an obvious random behavior.A high traffic density and a long maintenance period of a track will result in a substantial increase in the number of trains running on a bridge,and there is small likelihood that the maximum responses of the train and bridge happen in the total maintenance period of the track.Firstly,the coupling model of train–bridge systems is reviewed.Then,an ensemble method is presented,which can estimate the small probabilities of a dynamic system with stochastic excitations.The main idea of the ensemble method is to use the NARX(nonlinear autoregressive with exogenous input)model to replace the physical model and apply subset simulation with splitting to obtain the extreme distribution.Finally,the efficiency of the suggested method is compared with the direct Monte Carlo simulation method,and the probability exceedance of train responses under the vertical track irregularity is discussed.The results show that when the small probability of train responses under vertical track irregularity is estimated,the ensemble method can reduce both the calculation time of a single sample and the required number of samples. | Huoyue Xiang Ping Tang Yuan Zhang Yongle Li | 2020 | Railway Engineering Science2020，28，3： | 8 |
| 10 | High-throughput Binary Vectors for Plant Gene Function Analysis显示文摘一系列高产量的二进制克隆向量被构造在高等植物便于基因函数分析。这个向量系列为蛋白质本地化为工厂表示,倡导者分析,基因 silencing 和绿荧光灯的蛋白质熔化由 plasmidsdesigned 组成了。这些向量为 lambda 噬菌体 integrase/excisionase 提供了高产量、有效的克隆的利用地点。另外,我们在多重克隆再并入的唯一的限制地点地点和启用的倡导者代替。全部向量系列与完全的顺序信息是可得到的并且详细说明注解并且自由地为非商业性的使用被散布到科学界…… | Zhi-Yong Lei Ping Zhao Min-Jie Cao Rong Cui Xi Chen Li-Zhong Xiong Qi-Fa Zhang David J. Oliver Cheng-Bin Xiang | 2007 | Journal of Integrative Plant Biology2007，49，4： | 8 |
| 11 | Simultaneous desulfurization and denitrogen of liquid fuels using two functionalized group ionic liquids显示文摘Deep desulfurization of liquid fuels is an important and challenging issue in worldwide petroleum refining industry.Extraction and catalytic oxidative desulfurization(ECODS)of liquid fuels using a series of ionic liquids(ILs)with two functionalized groups,such as[(CH2)2COOHmim]Cl/n Fe Cl3,[(CH2)2COOHmim]Cl/n Zn Cl2,and[Amim]Cl/n Fe Cl3,was studied.In the ECODS,the ILs were used as both extractant and catalyst and 30 wt%hydrogen peroxide(H2O2)solution as oxidant.The effects of molar ratios of[(CH2)2COOHmim]Cl(or[Amim]Cl)to Fe Cl3(or Zn Cl2)in ILs,H2O2/sulfur(O/S)molar ratio,reaction temperature,and the nature of sulfur compounds on sulfur removal were investigated.The natures of the functional groups(–COOH,–CH2–CH=CH2)in cations and the acid strength of anions play important roles in the ECODS and affect the reaction time,temperature,and desulfurization efficiency of different substrates.Also,nitrogen-containing compounds(pyridine,pyrrole,and quinoline)could be removed simultaneously in the ECODS and had different effects on dibenzothiophene removal. | NIE Yi GONG Xue GAO Hong Shuai ZHANG Xiang Ping ZHANG Suo Jiang | 2014 | Science China Chemistry2014，57，12： | 8 |
| 12 | Quantum-inspired evolutionary tuning of SVM parameters显示文摘The most commonly used parameters selection method for support vector machines (SVM) is cross-validation, which needs a long- time complicated calculation. In this paper, a novel regularization parameter and a kernel parameter tuning approach of SVM are pre- sented based on quantum-inspired evolutionary algorithm (QEA). QEA with quantum chromosome and quantum mutation has better global search capacity. The parameters of least squares support vector machines (LS-SVM) can be adjusted using quantum-inspired evo- lutionary optimization. Classification and function estimation are studied using LS-SVM with wavelet kernel and Gaussian kernel. The simulation results show that the proposed approach can effectively tune the parameters of LS-SVM, and the improved LS-SVM with wavelet kernel can provide better precision. | Zhiyong Luo Ping Wang Yinguo Li Wenfeng Zhang Wei Tang Min Xiang | 2008 | Progress in Natural Science:Materials International2008，18，4： | 7 |
| 13 | An NT-3-releasing bioscaffold supports the formation of TrkC-modified neural stem cell-derived neural network tissue with efficacy in repairing spinal cord injury显示文摘The mechanism underlying neurogenesis during embryonic spinal cord development involves a specific ligand/receptor interaction,which may be help guide neuroengineering to boost stem cell-based neural regeneration for the structural and functional repair of spinal cord injury.Herein,we hypothesized that supplying spinal cord defects with an exogenous neural network in the NT-3/fibroin-coated gelatin sponge(NF-GS)scaffold might improve tissue repair efficacy.To test this,we engineered tropomyosin receptor kinase C(TrkC)-modified neural stem cell(NSC)-derived neural network tissue with robust viability within an NF-GS scaffold.When NSCs were genetically modified to overexpress TrkC,the NT-3 receptor,a functional neuronal population dominated the neural network tissue.The pro-regenerative niche allowed the long-term survival and phenotypic maintenance of the donor neural network tissue for up to 8 weeks in the injured spinal cord.Additionally,host nerve fibers regenerated into the graft,making synaptic connections with the donor neurons.Accordingly,motor function recovery was significantly improved in rats with spinal cord injury(SCI)that received TrkC-modified NSC-derived neural network tissue transplantation.Together,the results suggested that transplantation of the neural network tissue formed in the 3D bioactive scaffold may represent a valuable approach to study and develop therapies for SCI. | Ge Li Bao Zhang Jia-hui Sun Li-yang Shi Meng-yao Huang Li-jun Huang Zi-jing Lin Qiong-yu Lin Bi-qin Lai Yuan-huan Ma Bin Jiang Ying Ding Hong-bo Zhang Miao-xin Li Ping Zhu Ya-qiong Wang Xiang Zeng Yuan-shan Zeng | 2021 | Bioactive Materials2021，6，11： | 6 |
| 14 | Pathological changes in the lungs and lymphatic organs of 12 COVID-19 autopsy cases显示文摘Systematic autopsy and comprehensive pathological analyses of COVID-19 decedents should provide insights into the disease characteristics and facilitate the development of novel therapeutics.In this study,we report the autopsy findings from the lungs and lymphatic organs of 12 COVID-19 decedents—findings that evaluated histopathological changes;immune cell signature and inflammatory factor expression in the lungs,spleen and lymph nodes.Here we show that the major pulmonary alterations included diffuse alveolar damage,interstitial fibrosis and exudative inflammation featured with extensive serous and fibrin exudates,macrophage infiltration and abundant production of inflammatory factors(IL-6,IP-10,TNFo?and IL-I f).The spleen and hilar lymph nodes contained lesions with tissue structure disruption and immune cell dysregulation,including lymphopenia and macrophage accumulation.These findings provide pathological evidence that links injuries of the lungs and lymphatic organs with the fatal systematic respiratory and immune malfunction in critically ill COVID-19 patients. | Qian Liu Yu Shi Jun Cai Yaqi Duan Rongshuai Wang Hongyan Zhang Qiurong Ruan Jiansha Li Lei Zhao Yifang Ping Rong Chen Liang Ren Xiaochun Fei Heng Zhang Rui Tang Xi Wang Tao Luo Xindong Liu Xuequan Huang Zhenhua Liu Qilin Ao Yong Ren Jing Xiong Zhicheng He Haibo Wu Wenjuan Fu Pengnan Zhao Xinwei Chen Guoqiang Qu Yunyun Wang Xi Wang Jia Liu Dongfang Xiang Sanpeng Xu Xiaowei Zhou Qingrui Li Jinghong Ma Heng Li Jie Zhang Sizhe Huang Xiaohong Yao Yiwu Zhou Chaofu Wang Dingyu Zhang Guoping Wang Liang Liu Xiu-Wu Bian | 2020 | National Science Review2020，7，12： | 6 |
| 15 | Interactions and Biological Activity of Rare Earth Perchlorate Complexes with Alanine and Imidazole显示文摘The interactions of nine rare earth perchlorate complexes with alanine and imidazole were studied in methanol. The general formulae of these compounds are RE(Ala) 2Im(ClO 4) 3· n H 2O(RE=Ce, Pr, Nd, Sm, Eu, Gd, Tb, Yb) and La(Ala) 3Im(ClO 4) 3·H 2O, respectively. The composition and structure of the prepared compounds were determined by the chemical analysis, elemental analysis, IR spectroscopy, UV, FS spectroscopy, X ray powder diffraction, 1H NMR and solubility. The thermal decomposition processes of these complexes were studied on the basis of TG DTG. The preliminary bioassay shows that the compounds have a remarkable bacteriostasis effect on nine varieties of microbe. | HE Shui yang, CHEN Jun li, ZHANG Wei ping, LIU Xiang rong, SONG Di sheng ( Chemistry Department, North west University, Xi′an 710069, China) | 2001 | Journal of Rare Earths2001，19，1： | 5 |
| 16 | A Text Zero-Watermarking Algorithm Based on Chinese Phonetic Alphabets显示文摘The text watermarking is a feasible method to protect the copyright from being copied and tampered. In this paper, a text zero-watermarking algorithm is proposed based on the connection between the Chinese characters and the Chinese phonetic alphabets. According to the predefined interval threshold, the proposed algorithm extracts the characteristics of the text content by valuing on the basis of the custom of Chinese phonetic alphabets. After being chaotic transformed, the algorithm combines the text characteristics with the embedded watermarking information in the Chinese text. The experimental results show that the watermarking's capability of preventing tampering is up to 0.1%, which demonstrates the strong robustness and resistance to aggressive behavior of the algorithm. | ZHU Ping XIANG Guangli SONG Wenna LI Ankang ZHANG Yuexin TAO Ran | 2016 | Wuhan University Journal of Natural Sciences2016，21，4： | 5 |
| 17 | Long-term outcomes after radical gastrectomy in gastric cancer patients with overt bleeding显示文摘AIM: To investigate the difference in long-term outcomes between gastric cancer patients with and without a primary symptom of overt bleeding(OB).METHODS: Consecutive patients between January 1, 2007 and March 1, 2012 were identified retrospectively by reviewing a gastric cancer database at Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine. A follow-up examination was performed on patients who underwent a radical gastrectomy. OB due to gastric cancer included hematemesis, melena or hematochezia, and gastric cancer was confirmed as the source of bleeding by endoscopy. Patients without OB were defined as cases with occult bleeding and those with other initial presentations, including epigastric pain, weakness, weight loss and obstruction. The 3-year overall survival(OS) rate, age, gender, AJCC T stage, AJCC N stage, overall AJCC stage, tumor size, histological type, macroscopic(Borrmann) type, lymphovascular invasion and R status were compared between patients with and without OB. Moreover, we carried out a subgroup analysis based on tumor location(upper, middle and lower).RESULTS: We identified 939 patients. Of these, 695(74.0%) were hospitalized for potential radical gastrectomy and another 244 received palliative resection, rerouting of the gastrointestinal tract, chemotherapy, radiotherapy or no treatment due to the presence of unresectable tumors. Notably, there was no significant difference in the percentage of OB patients between resectable cases and unresectable cases(20.3 % vs 22.1 %, P = 0.541). Fo l l o w- u p examination was performed on 653 patients(94%) who underwent radical gastrectomy. We found no significant difference in 3-year OS rate(68.2% vs 61.2%, P = 0.143) or clinicopathological characteristics(P > 0.05) between these patients with and without OB. Subgroup analysis based on tumor location showed that the 3-year OS rate of upper gastric cancer was significantly higher in patients with OB(84.6%) than in those without OB(48.1%, P < 0.01) and that AJCC stagesⅠ-Ⅱ(56.4% vs 35.1%, P = 0.017) and T1-T2 category tumors(30.8% vs 13%, P = 0.010) were more frequent in patients with OB than in those without OB. There was no significant difference in 3-year OS rate or clinicopathological characteristics between patients with and without OB(P > 0.05) for middle or lower gastric cancer.CONCLUSION: Upper gastric cancer patients with OB exhibited tumors at less advanced pathological stages and had a better prognosis than upper gastric cancer patients without OB. | Lei Wang Xu-An Wang Jia-Qi Hao Li-Na Zhang Mao-Lan Li Xiang-Song Wu Hao Weng Wen-Jie Lv Wen-Jie Zhang Lei Chen Hong-Gang Xiang Jian-Hua Lu Ying-Bin Liu Ping Dong | 2015 | World Journal of Gastroenterology2015，21，47： | 5 |
| 18 | Genetic and Antibiotic Resistance Characteristics ofCampylobacterjejuni Isolated from Diarrheal Patients,Poultry and Cattle in Shenzhen显示文摘Objective To investigate genetic and antibiotic resistance characteristics of Campylobacter jejuni(C. jejuni) isolated from Shenzhen. Methods Multilocs sequence typing and agar dilution methods were used to define the genotype and antibiotic resistance of C. jejuni, respectively. Results In total, 126 C. jejuni strains were isolated. The prevalence of C. jejuni was 5.3% in diarrheal patients. The prevalence in poultry meat(36.5%) was higher than that in cattle meat(1.1%). However, the prevalence in poultry cloacal swabs(27.0%) was lower than that in cattle stool(57.3%). Sixty-two sequence types were obtained, among which 27 of the STs and 10 alleles were previously unreported. The most frequently observed clonal complexes were ST-21(11.9%), ST-22(10.3%), and ST-403(7.1%). ST-21, ST-45, ST-354, ST-403, and ST-443 complexes overlapped between isolates from patients and cattle, whereas ST-45 and ST-574 complexes overlapped between isolates from patients and poultry. All C. jejuni were resistant to at least one antibiotic. The highest resistance rate was toward ciprofloxacin(89.7%), followed by tetracycline(74.6%), and nalidixic acid(69.0%). Conclusion This is the first report of the genotypes and antibiotic resistance of C. jejuni in Shenzhen. Overlapping clonal complexes were found between isolates from patients and cattle, and between patients and poultry. | JU Chang Yan ZHANG Mao Jun MA Yan Ping LU Jing Rang YU Mu Hua CHEN Hui LIU Chu Yun GU Yi Xin FU Yan Yan DUAN Yong Xiang | 2018 | Biomedical and Environmental Sciences2018，31，8： | 5 |
| 19 | Application of UPLC-MS/MS Method for Analyzing B-vitamins in Human Milk显示文摘Objective To determine ten B-vitamins in human milk by ultra-high performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS). Methods The pretreated human milk samples were adequately separated and quantified within 11 min by UPLC-MS/MS with an Acquity UPLC HSS T3 column(2.1×100 mm, 1.8 μm). The mobile phase was a gradient of 2.5 mmol/L ammonium formate aqueous solution and acetonitrile at a flow rate of 0.35 m L/min. Stable isotope internal standards were used in the analysis, to correct for the method variability, including matrix and ionization effects. The homogenized human milk samples were deproteinzed using methanol, unknown contaminants were extracted with diethyl ether and hydrophobic phase was discarded. The analytes were monitored via ESI+ionization and detected in multiple reaction monitoring(MRM) with three acquisition functions. Results Calibration curves ranged from 0.5-160 ng/m L(thiamin, riboflavin, biotin, nicotinic acid, pyridoxine, pyridoxamine, pyridoxal), and 2.5-800 ng/m L(pantothenic acid, FAD and nicotinamide)(R2=0.990-0.999). The relative recovery ranged from 80.1% to 120.2%; accuracy was determined to be 98.3% to 108.0%. Intra-day and inter-day variation were 3.4%-19.9% and 5.9%-18.1%, respectively. The limit of quantification(LOQ) for all vitamins was between 0.25 and 3 μg/L. Conclusion This method was successfully applied for simultaneous analysis of ten B-vitamins in human milk. | REN Xiang Nan YIN Shi An YANG Zhen Yu YANG Xiao Guang SHAO Bing REN Yi Ping ZHANG Jing | 2015 | Biomedical and Environmental Sciences2015，28，10： | 4 |
| 20 | Ciprofloxacin blocked enterohepatic circulation of diclofenac and alleviated NSAID-induced enteropathy in rats partly by inhibiting intestinal 13-glucuronidase activity显示文摘瞄准:Diclofenac 是 non-steroidal 反煽动性的药(NSAID ) ,它可以引起严肃的肠的不利反应(enteropathy ) 。在这研究,我们调查了 ciprofloxacin 的合作管理是否在 rats.Methods 影响了 diclofenac 和导致 diclofenac 的 enteropathy 的 pharmacokinetics:diclofenac 的 pharmacokinetics 在收到 diclofenac 以后在老鼠被估计(10 mg/kg, ig,或 5 mg/kg, iv ) ,与或没有 ciprofloxacin (20 mg/kg, ig ) 共同管理。在收到 6 口头的剂量或 diclofenac 的 15 静脉内的剂量以后,老鼠被牺牲,并且小肠被移开检验导致 diclofenac 的 enteropathy。β在肠的满意的、牛的肝和 E coli 的 -Glucuronidase 活动是 evaluated.Results:后面的口头或静脉内的管理, diclofenac 的 pharmacokinetic 侧面显示了废除的 ciprofloxacin 的典型 enterohepatic 循环,和合作管理 enterohepatic 发行量,在 diclofenac 的血浆内容导致了重要减小。在控制老鼠, β在小肠的内容的 -glucuronidase 活动是区域依赖者:近似 intestine<远侧的 intestine< ileal 阀门。ciprofloxac 的管理引起了 β 的重要减小;在远侧的小肠,并且特别地在 ileal 的 -glucuronidase 活动阀门。而且, ciprofloxacin (10-2000 μ mol/L ) dose-dependently 禁止的 β在远侧的小肠内容的 -glucuronidase 活动或 E coli 在 vitro 孵化了,但是没在在 vitro 孵化的近似的小肠内容或牛的肝影响那。在收到 6 口头的剂量或 diclofenac 的 15 静脉内的剂量以后,典型 enteropathy 与严重 enteropathy 被开发发生在远侧的小肠。ciprofloxacin 的合作管理显著地减轻了导致 diclofenac 的 enteropathy.Conclusion:ciprofloxacin 的合作管理稀释了 diclofenac 的 enterohepatic 循环并且在老鼠减轻了导致 diclofenac 的 enteropathy,部分经由肠的 β 的抑制; -glucuronidase 活动。 | Ze-yu ZHONG Bin-bin SUN Nan SHU Qiu-shi XIE Xiange TANG Zhao-li LING Fan WANG Kai-jing ZHAO Ping XU Mian ZHANG Ying LI Yang CHEN Li LIU Lun-zhu XIA Xiao-dong LIU | 2016 | Acta Pharmacologica Sinica2016，37，7： | 4 |
您所在的机构暂未开通此部分的功能，请联系管理员