|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Pantograph and catenary system with double pantographs for high-speed trains at 350 km/h or higher显示文摘The paper is aimed at developing an optimized design of the pantograph and catenary system with double pantographs at a speed of 350 km/h for the Wuhan-Guangzhou high-speed railway.First,the pantograph and catenary system for the Beijing-Tianjin high-speed railway was analyzed to verify whether its design objective could be fulfilled.It shows that the system is not able to satisfy the requirement of a sustainable running speed of 350 km/h.Then a new scheme for the pantograph and catenary system is proposed through optimization and renovation of the structure and parameters of the pantograph and catenary system,including the suspension type of the catenary,tension of the contact wire,and space between two pantographs.Finally,the dynamic performance of the new system was verified by simulation and line testing.The results show that the new scheme of the pantograph and catenary system for the WuhanGuangzhou high-speed railway is acceptable,in which the steady contact between the rear pantograph and the catenary at the space of 200 m can be maintained to ensure the current-collection quality.A current collection with double pantographs at a speed of 350 km/h or higher can be achieved. | Weihua ZHANG Ning ZHOU Ruiping LI Guiming MEI Dongli SONG | 2011 | Journal of Modern Transportation2011，19，1： | 8 |
| 2 | New discoveries on the effects of desertification on the ground temperature of permafrost and its significance to the Qinghai-Tibet Plateau显示文摘The desert and permafrost conditions of the Qinghai-Tibet Plateau are unique.However,the effects of desertification on the ground temperature of permafrost are currently unclear.Recently,understanding this problem has become more urgent because of increasing desertification on the plateau.For this reason,an observational field experiment was undertaken by the authors at Honglianghe on the Qinghai-Tibet Plateau.Thermistor ground temperature probes were used,and synchronized contrasting observations were made in an open area.Observations of the ground temperature of permafrost below sand layers with a range of thicknesses were made from May 2010 to April 2011.The sand layers were found to play a key role in the protection of the underlying permafrost.The ground temperature below a permafrost table overlain by a thick sand layer was lower than that of the average annual temperature for the natural ground surface,and the temperature drop was roughly constant at 0.2°C.During the warmer part of the year (May to September),the maximum temperature drops over the five months were 3.40,3.72,4.85,3.16,and 1.88°C,respectively.The ground temperature near a permafrost table overlain by a thin sand layer was also lower than that of the average annual temperature for the natural ground surface.However,in this case the average of the annual maximum temperature drop was significantly less,0.71°C.The scientific significance of our preliminary conclusions is not only to present an exploration of the interaction between desertification and permafrost,but also to provide new engineering ideas for protecting the permafrost in regions where construction is required on the Qinghai-Tibet Plateau. | XIE ShengBo QU JianJun ZU RuiPing ZHANG KeCun HAN QingJie | 2012 | Chinese Science Bulletin2012，57，8： | 8 |
| 3 | Review of pantograph and catenary interaction显示文摘 | Weihua ZHANG Dong ZOU Mengying TAN Ning ZHOU Ruiping LI Guiming MEI | 2018 | Frontiers of Mechanical Engineering2018，13，2： | 6 |
| 4 | Effect of microstructure on the mechanical and corrosion behaviors of a hot-extruded nickel aluminum bronze显示文摘In this paper,the influence of microstructure on the corrosion behavior of a hotextruded nickel aluminum bronze was studied.Three kinds of samples subjected to the hot-extrusion,annealing and quenching conditions were prepared and immersion tests in 3.5% NaCl solution were carried out.Microstructures and corrosion surface morphologies of the samples were observed by SEM.It was found that the retained β martensite and(α+κⅢ) lamella eutectoid in the as hot-extruded material were eliminated after annealing,and corrosion resistance of the alloy was improved.As to the as-quenched material,its corrosion rate was higher than that of the as hotextruded material since the volume fraction of β phase which was anodic to α phase increased after quenching.As regards the mechanical properties,the as-quenched sample possesses the highest hardness and tensile strength among the three kinds of samples,while the as-annealed sample possesses the highest elongation.That is to say,heat treatment plays different roles in the mechanical properties and corrosion resistance of the experimental alloy. | Datong ZHANG Ruiping CHEN Weiwen ZHANG Zongqiang LUO Yuanyuan LI | 2010 | Acta Metallurgica Sinica(English Letters)2010，23，2： | 5 |
| 5 | Rapid and sensitive liquid chromatography–tandem mass spectrometric method for the quantitative determination of potentially harmful substance 5,5′-oxydimethylenebis (2-furfural) in traditional Chinese medicine injections显示文摘With the rapid development and wide application of traditional Chinese medicine injection(TCMI), a number of adverse events of some TCMIs have incessantly been reported and have drawn broad attention in recent years. Establishing effective and practical analytical methods for safety evaluation and quality control of TCMI can help to improve the safety of TCMIs in clinical applications. In this study, a sensitive and rapid high-performance liquid chromatography–tandem mass spectrometry(HPLC–MS/MS)method has been developed and validated for the quantitative determination of potentially harmful substance5,5′-oxydimethylenebis(2-furfural, OMBF) in TCMI samples. Chromatographic separation was performed on a C18 reversed-phase column(150 mm × 2.1 mm, 5 μm) by gradient elution, using methanol–water containing 0.1% formic acid as mobile phase at the flow rate of 0.3 m L/min. MS/MS detection was performed on a triple quadrupole mass spectrometer with positive electrospray ionization in the multiple reaction-monitoring mode. The method was sensitive with a limit of quantification of 0.3 ng/m L and linear over the range of 0.3–30 ng/m L(r = 0.9998). Intra-and inter-day precision for analyte was o9.52% RSD withrecoveries in the range 88.0–109.67% at three concentration levels. The validated method was successfully applied to quantitatively determine the compound OMBF in TCMIs and glucose injections. Our study indicates that this method is simple, sensitive, practicable and reliable, and could be applied for safety evaluation and quality control of TCMIs and glucose injections. | Qingce Zang Yang Gao Luojiao Huang Jiuming He Sheng Lin Hongtao Jin Ruiping Zhang Zeper Abliz | 2018 | Acta Pharmaceutica Sinica B2018，8，2： | 4 |
| 6 | 3D-Printed Superhydrophobic and Magnetic Device That Can Self-Powered Sense A Tiny Droplet Impact显示文摘Three-dimensional(3D)-printed magnetic soft architectures have attracted extensive attention and research from the engineering and material fields.The force-driven shape deformation of such architectures causes a change in the magnetic field distribution,indicating the capability to convert mechanical energy to electricity.Herein,we fabricate a flexible superhydrophobic and magnetic device by integrating two kinds of 3D printing approaches.The 3D-printed magnetic device(3DMD)exhibits a long-term stable mechanoelectrical conversion capacity under consecutive water droplet dripping.The output current of the 3DMD is higher than that of records in the existing literature.Combined with Maxwell numerical simulation,the mechanoelectrical conversion mechanism of the 3DMD is investigated,further guiding regulation of the diverse parameters.Moreover,three 3DMDs are integrated to light up a commercial light-emitting diode(LED)by a stream of collected rainwater.Such a combined design incorporating energy conversion is believed to promisingly motive advances in the 3D printing field. | Xuan Zhang Qi Wang Ruiping Zou Bo Song Chunze Yan Yusheng Shi Bin Su | 2022 | Engineering2022，8，8： | 4 |
| 7 | Sn02 nanoparticles anchored on graphene oxide as advanced anode materials for high-performance lithium-ion batteries显示文摘Lithium-ion batteries (LIBs) with high energy density have attracted great attention for their wide applications in electric vehicles, and the exploration of the nextgeneration anode materials with high theoretical capacity is highly desired. In this work, Sn02 nanoparticles with the particle size of 200 nm uniformly anchored on the surface of graphene oxide (GO) was prepared by combination of the ultrasonic method and the following calcination process. The Sn02/GO composite with the weight ratio of Sn02 to GO at 4:1 exhibits excellent electrochemical performance, which originates from the synergistic effects between GO and Sn02 nanoparticles. A high discharge capacity of 492 mA·h·g^-1 can be obtained after 100 cycles at 0.2C, and after cycling at higher current densities of 1C and 2C, a discharge capacity of 641 mA·h·g^-1 can be restored when the current density goes back to 0.1C. The superior electrochemical performance and simple synthesis process make it a very promising candidate as anode materials for LIBs. | Ruiping LIU Ning ZHANG Xinyu WANG Chenhui YANG Hui CHENG Hanqing ZHAO | 2019 | Frontiers of Materials Science2019，13，2： | 4 |
| 8 | In vivo tracking of neuronal-like cells by magnetic resonance in rabbit models of spinal cord injury显示文摘In vitro experiments have demonstrated that neuronal-like cells derived from bone marrow mesenchymal stem cells can survive,migrate,integrate and help to restore the function and behaviors of spinal cord injury models,and that they may serve as a suitable approach to treating spinal cord injury.However,it is very difficult to track transplanted cells in vivo.In this study,we injected superparamagnetic iron oxide-labeled neuronal-like cells into the subarachnoid space in a rabbit model of spinal cord injury.At 7 days after cell transplantation,a small number of dot-shaped low signal intensity shadows were observed in the spinal cord injury region,and at 14 days,the number of these shadows increased on T2-weighted imaging.Perl’s Prussian blue staining detected dot-shaped low signal intensity shadows in the spinal cord injury region,indicative of superparamagnetic iron oxide nanoparticle-labeled cells.These findings suggest that transplanted neuronal-like cells derived from bone marrow mesenchymal stem cells can migrate to the spinal cord injury region and can be tracked by magnetic resonance in vivo.Magnetic resonance imaging represents an efficient noninvasive technique for visually tracking transplanted cells in vivo. | Ruiping Zhang Kun Zhang Jianding Li Qiang Liu Jun Xie | 2013 | Neural Regeneration Research2013，8，36： | 4 |
| 9 | Elastic Scattering of 7Be on Pb Target显示文摘 | Yang Yanyun Wang Jiansong Wang Qi Huang Meirong Ma Junbing Ma Peng Cao Xiguang Han Jianlong Bai Zhen Jin Shilong Hu Qiang Jin Lei Chen Jiangbo Zhang Xueying Chen Ruofu Hu Zhengguo Xu Shiwei Zhen Chuan Xu Zhiguo Xu Hushan Duan Liming Sun Zhiyu Chen Zhiqiang He Jianjun Li Songlin Lei Xiangguo Fu Fen Zhang Xueheng Yuan Xiaohua Du Chengming Yan Xinshuang Hu Jun Tang Shuwen Ye Ruiping Yang Kun Shi Fudong Zhang Wei Chen Ze Li Long Xu Xing Liu Longxiang Zhang Liyong Lin Qingyun Gou Boxing Xu Ge Zhou Xionghong Zhang Yuhu Xiao Guoqing | 2011 | IMP & HIRFL Annual Report2011，，1： | 4 |
| 10 | Microstructural evolution and defect formation in a powder metallurgy nickel-based superalloy processed by selective laser melting显示文摘In this study,the selective laser melting(SLM)technology has been employed to manufacture a nickelbased superalloy which was conventionally prepared through powder metallurgy(PM)route.The microstructural features and defects were systematically investigated both prior to and after heat treatment and compared with the PM counterpart.Both solidification cracking and liquation cracking were observed in the SLM specimen in which the grain misorientation and low melting point(γ+γ')eutectic played a vital role in their formation mechanism.Columnar grains oriented along building direction were ubiquitous,corresponding to strong<001>fiber texture.Solidification cell structures and melt pools are pervasive and noγ'precipitates were detected at about 10 nm scale before heat treatment.After supersolvus solution and two-step aging treatments,high volume fractionγ'precipitates emerged and their sizes and morphologies were comparable to those in PM alloy.<001>texture is relieved and columnar grains tend to become more equiaxed due to static recrystallization process and grain boundary migration events.Significant annealing twins formed in SLM alloy and are clarified as a consequence of recrystallization.Our results provide fundamental understandings for the SLM PM nickel-based superalloy both before and after heat treatment and demonstrate the potential to fabricate this group of alloys using SLM technology. | Hongyu Wu Dong Zhang Biaobiao Yang Chao Chen Yunping Li Kechao Zhou Liang Jiang Ruiping Liu | 2020 | Journal of Materials Science & Technology2020，36，1： | 4 |
| 11 | Oral microbiome and risk of malignant esophageal lesions in a high-risk area of China:A nested case-control study显示文摘Objective:We aimed to prospectively evaluate the association of oral microbiome with malignant esophageal lesions and its predictive potential as a biomarker of risk.Methods:We conducted a case-control study nested within a population-based cohort with up to 8 visits of oral swab collection for each subject over an 11-year period in a high-risk area for esophageal cancer in China.The oral microbiome was evaluated with 16 S ribosomal RNA(rRNA)gene sequencing in 428 pre-diagnostic oral specimens from 84 cases with esophageal lesions of severe squamous dysplasia and above(SDA)and 168 matched healthy controls.DESeq analysis was performed to identify taxa of differential abundance.Differential oral species together with subject characteristics were evaluated for their potential in predicting SDA risk by constructing conditional logistic regression models.Results:A total of 125 taxa including 37 named species showed significantly different abundance between SDA cases and controls(all P<0.05&false discovery rate-adjusted Q<0.10).A multivariate logistic model including 11 SDA lesion-related species and family history of esophageal cancer provided an area under the receiver operating characteristic curve(AUC)of 0.89(95%CI,0.84-0.93).Cross-validation and sensitivity analysis,excluding cases diagnosed within 1 year of collection of the baseline specimen and their matched controls,or restriction to screenendoscopic-detected or clinically diagnosed case-control triads,or using only bacterial data measured at the baseline,yielded AUCs>0.84.Conclusions:The oral microbiome may play an etiological and predictive role in esophageal cancer,and it holds promise as a non-invasive early warning biomarker for risk stratification for esophageal cancer screening programs. | Fangfang Liu Mengfei Liu Ying Liu Chuanhai Guo Yunlai Zhou Fenglei Li Ruiping Xu Zhen Liu Qiuju Deng Xiang Li Chaoting Zhang Yaqi Pan Tao Ning Xiao Dong Zhe Hu Huanyu Bao Hong Cai Isabel Dos Santos Silva Zhonghu He Yang Ke | 2020 | Chinese Journal of Cancer Research2020，32，6： | 3 |
| 12 | Carbon-based flexible self-supporting cathode for lithium-sulfur batteries:Progress and perspective显示文摘The flexible self-supporting electrode can maintain good mechanical and electrical properties while retaining high specific capacity,which meets the requirements of flexible batteries.Lithium-sulfur batteries(LSBs),as a new generation of energy storage system,hold much higher theoretical energy density than traditional batteries,and they have attracted extensive attention from both the academic and industrial communities.Selection of a proper substrate material is important for the flexible self-supporting electrode.Carbon materials,with the advantages of light weight,high conductivity,strong structural plasticity,and low cost,provide the electrode with a large loading space for the active material and a conductive network.This makes the carbon materials meet the mechanical and electrochemical requirements of flexible electrodes.In this paper,the commonly used fabrication methods and recent research progresses of the flexible self-supporting cathode with a carbon material as the substrate are introduced.Various sulfur loading methods are summarized,which provides useful information for the structural design of the cathode.As the first review article of the carbon-based flexible self-supporting LSB cathodes,it provides valuable guidance for the researchers working in the field of LSB. | Qinghuiqiang Xiao Jinlin Yang Xiaodong Wang Yirui Deng Peng Han Ning Yuan Lei Zhang Ming Feng Chang‐an Wang Ruiping Liu | 2021 | Carbon Energy2021，3，2： | 3 |
| 13 | Hollow nanoparticles synthesized via Ostwald ripening and their upconversion luminescence-mediated Boltzmann thermometry over a wide temperature range显示文摘Upconversion nanoparticles(UCNPs)with hollow structures exhibit many fascinating optical properties due to their special morphology.However,there are few reports on the exploration of hollow UCNPs and their optical applications,mainly because of the difficulty in constructing hollow structures by conventional methods.Here,we report a one-step template-free method to synthesize NaBiF4:Yb,Er(NBFYE)hollow UCNPs via Ostwald ripening under solvothermal conditions.Moreover,we also elucidate the possible formation mechanism of hollow nanoparticles(HNPs)by studying the growth process of nanoparticles in detail.By changing the contents of polyacrylic acid and H2O in the reaction system,the central cavity size of NBFYE nanoparticles can be adjusted.Benefiting from the structural characteristics of large internal surface area and high surface permeability,NBFYE HNPs exhibit excellent luminescence properties under 980 nm near-infrared irradiation.Importantly,NBFYE hollow UCNPs can act as self-referenced ratiometric luminescent thermometers under 980 nm laser irradiation,which are effective over a wide temperature range from 223 K to 548 K and have a maximum sensitivity value of 0.0065 K^(−1)at 514 K.Our work clearly demonstrates a novel method for synthesizing HNPs and develops their applications,which provides a new idea for constructing hollow structure UCNPs and will also encourage researchers to further explore the optical applications of hollow UCNPs. | Ran An Yuan Liang Ruiping Deng Pengpeng Lei Hongjie Zhang | 2022 | Light(Science & Applications)2022，11，8： | 3 |
| 14 | Identification of cancer patients using claims data from health insurance systems: A real-world comparative study显示文摘Objective: To evaluate the accuracy of identifying cancer patients by use of medical claims data in a health insurance system in China, and provide the basis for establishing the claims-based cancer surveillance system in China.Methods: We chose Hua County, Henan Province as the study site, and randomly selected 300 and 1,200 qualified inpatient electronic medical records(EMRs) as well as the New Rural Cooperative Medical Scheme(NCMS) claims records for cancer patients in Hua County People’s Hospital(HCPH) and Anyang Cancer Hospital(ACH) in 2017. Diagnostic information for NCMS claims was evaluated on an individual level, and sensitivity and positive predictive value(PPV) were calculated taking the EMRs as the gold standard.Results: The sensitivity of NCMS was 95.2%(93.8%-96.3%) and 92.0%(88.3%-94.8%) in ACH and HCPH,respectively. The PPV of the NCMS was 97.8%(96.7%-98.5%) in ACH and 89.0%(84.9%-92.3%) in HCPH.Overall, the weighted and combined sensitivity and PPV of NCMS in Hua County was 93.1% and 92.1%,respectively. Significantly higher sensitivity and PPV in identifying patients with common cancers than noncommon cancers were detected in HCPH and ACH separately(P<0.01).Conclusions: Identification of cancer patients by use of the NCMS is accurate on individual level, and it is therefore feasible to conduct claims-based cancer surveillance in areas not covered by cancer registries in China. | Hongrui Tian Ruiping Xu Fenglei Li Chuanhai Guo Lixin Zhang Zhen Liu Mengfei Liu Yaqi Pan Zhonghu He Yang Ke | 2019 | Chinese Journal of Cancer Research2019，31，4： | 3 |
| 15 | Association among lifestyle, clinical examination, polymorphisms in CDH1 gene and Traditional Chinese Medicine syndrome differentiation of gastric cancer显示文摘OBJECTIVE:To explore the association among lifestyle,clinical examination,polymorphisms in CDH1 gene and Traditional Chinese Medicine(TCM)syndrome differentiation of gastric cancer(GC). METHODS:A hospital-based population of 387 GC patients was investigated in Jiangsu province.Relevant information regarding lifestyle and clinical examination were collected by a standard questionnaire.Four known single nucleotide polymorphisms(SNPs)in CDH1 were investigated by polymerase chain reaction-ligation detection reaction methods.Statistical analysis was conducted by SPSS 16.0 software.RESULTS:The results showed that meal duration and the status of glutamic pyruvic transaminase were significantly associated with TCM syndrome differentiation of GC(both P<0.05).None of the four SNPs in the E-cadherin(CDH1)gene achieved significant differences in their distributions among the nine syndrome types of GC(both P>0.05).However,significant differences were observed in rs13689 genotype distributions between several pairs of syndrome types of GC,suggesting that rs13689 is correlated with the syndrome differentiation of GC.CONCLUSION:Integrated analysis of lifestyle,clinical examination and CDH1 gene polymorphisms can contribute to a better understanding of the GC syndrome types and may improve the efficacy of interventions by stratifying disease according to TCM criteria. | Junfeng Zhang Zhen Zhan Juan Wu Chunbing Zhang Yaping Yang Shujuan Tong Ruiping Wang Xuewen Yang Wei Dong | 2013 | Journal of Traditional Chinese Medicine2013，33，5： | 3 |
| 16 | Wind tunnel simulation experiment and investigation on the electrification of sandstorms显示文摘Electric discharge phenomenon is often observed during the passage of sandstorms over desert regions and sometimes does great damage to human beings. Based on previous researches on the electrification of sandstorms by the scholars both at home and aboard,the authors conducted the simulation experiments on the electric fields of sandstorm-triggered creeping-saltating sand, suspending sand and watered suspending sand in the large-scale wind tunnel. According to the experimental results the electric field structure and its formation mechanism were analysed and discussed, the quantity of electric charges and electric field strengthwere measured and calculated, and finally several conclusions were drawn. | QU Jianjun YAN Muhong DONG Guangrong ZHANG Hongfa ZU Ruiping TUO Wanquan ZHAO Aiguo XIAO Zhenghua LI Fang YANG Bao | 2004 | Science China Earth Sciences2004，47，6： | 2 |
| 17 | A high-performance bio-tissue imaging method using air flow-assisted desorption electrospray ionization coupled with a high-resolution mass spectrometer显示文摘Mass spectrometry imaging (MSI) technology can simultaneously obtain the spatial distribution of thousands of chemical compounds and has unique advantages compared to other techniques that allow mapping the surface of bio-tissue. Here, we combined an air flow-assisted desorption electrospray ionization (AFADESI) MSI device with a high-resolution mass spectrometer to optimize the system parameters and achieve more accurate spatial distribution characteristics for compounds of interest while investigating bio-tissue sections. The platform set-up, required instrumentation, sample pretreatment, parameter optimization and bio-tissue characterization are described and discussed.Finally, the parameter conditions that can provide optimal ionic intensity and enhanced resolution were confirmed. The reasonable resolution and sensitivity improvements of AFADESI-MSI have been achieved through tandem a high-resolution mass spectrometer system, therefore, it would be a promising technique for the bio-tissue imaging analysis. | Yiwei Lv Tiegang Li Chengan Guo Chenglong Sun Fei Tang Luojiao Huang Zhigang Luo Xin Li Ruiping Zhang Qingce Zang Jiuming He Zeper AbliZ | 2019 | Chinese Chemical Letters2019，30，2： | 2 |
| 18 | Utilization of aluminum hydroxide waste generated in fluoride adsorption and coagulation processes for adsorptive removal of cadmium ion显示文摘尽管基于 Al 的凝结和吸附过程为氟化物(F) 移动被证明高度有效,二个过程两个都产生大量艾尔(哦)3 固体废物包含 F (艾尔(哦)3-F) 。这研究试图调查利用艾尔的可行性(哦) 在艾尔产生的 3-F (哦)3 吸附(艾尔(哦)3-F 广告 ) 和凝结(艾尔(哦)3-F 为镉离子(Cd (II )) 的吸附的 coag ) 。艾尔的吸附能力(哦)3-F 广告 和艾尔(哦)3-F 为 Cd (II ) 的 coag 作为太古的铝氢氧化物的类似(艾尔(哦) 具有 24.39 和 19.90 mg 映慬Р的 3), | Jiawei JU Ruiping LIU Zan HE Huijuan LIU Xiwang ZHANG Jiuhui QU | 2016 | Frontiers of Environmental Science & Engineering2016，10，3： | 2 |
| 19 | Advanced noble-metal-free bifunctional electrocatalysts for metal-air batteries显示文摘The sluggish reaction kinetics at the oxygen cathode is one of the important issues hindering the commercialization of the metal-air batteries.Although the noble metal can be used as the high-efficiency electrocatalyst to solve the problems to some extent,the high cost and scarcity of these noble-metal catalysts have limited their application in electrocatalysis.In this review,we discussed the mecha-nisms of the ORR and OER,and proposed the principles for the bifunctional electrocatalysts firstly,and then the state-of-the-art bifunctional catalysts,including carbon-based materials and transition-metal-based materials.On the basis of that,the self-supporting 3D noble-metal-free bifunctional ORR/OER catalysts were also discussed.Finally,the perspectives for the bifunctional electrocatalysts were discussed. | Wenhao Tang Boya Li Kewei Teng Xiaodong Wang Ruiping Liu Mengwei Wu Lei Zhang Pengfei Ren Junqing Zhang Ming Feng | 2022 | Journal of Materiomics2022，8，2： | 2 |
| 20 | Spatiotemporal pharmacometabolomics based on ambient mass spectrometry imaging to evaluate the metabolism and hepatotoxicity of amiodarone in HepG2 spheroids显示文摘Three-dimensional(3D)cell spheroid models combined with mass spectrometry imaging(MSI)enables innovative investigation of in vivo-like biological processes under different physiological and pathological conditions.Herein,airflow-assisted desorption electrospray ionization-MSI(AFADESI-MSI)was coupled with 3D HepG2 spheroids to assess the metabolism and hepatotoxicity of amiodarone(AMI).High-coverage imaging of>1100 endogenous metabolites in hepatocyte spheroids was achieved using AFADESI-MSI.Following AMI treatment at different times,15 metabolites of AMI involved in Ndesethylation,hydroxylation,deiodination,and desaturation metabolic reactions were identified,and according to their spatiotemporal dynamics features,the metabolic pathways of AMI were proposed.Subsequently,the temporal and spatial changes in metabolic disturbance within spheroids caused by drug exposure were obtained via metabolomic analysis.The main dysregulated metabolic pathways included arachidonic acid and glycerophospholipid metabolism,providing considerable evidence for the mechanism of AMI hepatotoxicity.In addition,a biomarker group of eight fatty acids was selected that provided improved indication of cell viability and could characterize the hepatotoxicity of AMI.The combination of AFADESI-MSI and HepG2 spheroids can simultaneously obtain spatiotemporal information for drugs,drug metabolites,and endogenous metabolites after AMI treatment,providing an effective tool for in vitro drug hepatotoxicity evaluation. | Limei Li Qingce Zang Xinzhu Li Ying Zhu Shanjing Wen Jiuming He Ruiping Zhang Zeper Abliz | 2023 | Journal of Pharmaceutical Analysis2023，13，5： | 2 |
您所在的机构暂未开通此部分的功能，请联系管理员