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| 1 | Genome-wide Targeted Mutagenesis in Rice Using the CRISPR/Cas9 System显示文摘 | Lu, Yuming Ye, Xiao Guo, Renming Huang, Jing Wang, Wei Tang, Jiuyou Tan, Longtao Zhu, Jian-kang Chu, Chengcai Qian, Yangwen | 2017 | Molecular Plant2017，10，9： | 28 |
| 2 | The Interactions among DWARF10,Auxin and Cytokinin Underlie Lateral Bud Outgrowth in Rice显示文摘Previous studies have shown that DWARF10(D10) is a rice ortholog of MAX4/RMS1/DAD1,encoding a carotenoid cleavage dioxygenase and functioning in strigolactones/strigolactone-derivatives(SL) biosynthesis.Here we use D10-RNA interference(RNAi) transgenic plants similar to d10 mutant in phenotypes to investigate the interactions among D10,auxin and cytokinin in regulating rice shoot branching.Auxin levels in node 1 of both decapitated D10-RNAi and wild type plants decreased significantly,showing that decapitation does reduce endogenous auxin concentration,but decapitation has no clear effects on auxin levels in node 2 of the same plants.This implies that node 1 may be the location where a possible interaction between auxin and D10 gene would be detected.D10 expression in node 1 is inhibited by decapitation,and this inhibition can be restored by exogenous auxin application,indicating that D10 may play an important role in auxin regulation of SL.The decreased expression of most OsPINs in shoot nodes of D10-RNAi plants may cause a reduced auxin transport capacity.Furthermore,effects of auxin treatment of decapitated plants on the expression of cytokinin biosynthetic genes suggest that D10 promotes cytokinin biosynthesis by reducing auxin levels.Besides,in D10-RNAi plants,decreased storage cytokinin levels in the shoot node may partly account for the increased active cytokinin contents,resulting in more tillering phenotypes. | Shuying Zhang Gang Li Jun Fang Weiqi Chen Haipai Jiang Junhuang Zou Xue Liu Xianfeng Zhao Xiaobing Li Chengcai Chu Qi Xie Xiangning Jiang Lihuang Zhu | 2010 | Journal of Integrative Plant Biology2010，52，7： | 14 |
| 3 | Analysis of genetic architecture and favorable allele usage of agronomic traits in a large collection of Chinese rice accessions显示文摘Genotyping and phenotyping large natural populations provide opportunities for population genomic analysis and genome-wide association studies(GWAS). Several rice populations have been re-sequenced in the past decade;however, many major Chinese rice cultivars were not included in these studies. Here, we report large-scale genomic and phenotypic datasets for a collection mainly comprised of 1,275 rice accessions of widely planted cultivars and parental hybrid rice lines from China. The population was divided into three indica/Xian and three japonica/Geng phylogenetic subgroups that correlate strongly with their geographic or breeding origins. We acquired a total of 146 phenotypic datasets for 29 agronomic traits under multi-environments for different subpopulations. With GWAS, we identified a total of 143 significant association loci, including three newly identified candidate genes or alleles that control heading date or amylose content. Our genotypic analysis of agronomically important genes in the population revealed that many favorable alleles are underused in elite accessions, suggesting they may be used to provide improvements in future breeding efforts. Our study provides useful resources for rice genetics research and breeding. | Xiuxiu Li Zhuo Chen Guomin Zhang Hongwei Lu Peng Qin Ming Qi Ying Yu Bingke Jiao Xianfeng Zhao Qiang Gao Hao Wang Yunyu Wu Juntao Ma Liyan Zhang Yongli Wang Lingwei Deng Shanguo Yao Zhukuang Cheng Diqiu Yu Lihuang Zhu Yongbiao Xue Chengcai Chu Aihong Li Shigui Li Chengzhi Liang | 2020 | Science China(Life Sciences)2020，63，11： | 8 |
| 4 | From Green Super Rice to green agriculture:Reaping the promise of functional genomics research显示文摘Producing sufficient food with finite resources to feed the growing global population while having a smaller impact on the environment has always been a great challenge.Here,we review the concept and practices of Green Super Rice(GSR)that have led to a paradigm shift in goals for crop genetic improvement and models of food production for promoting sustainable agriculture.The momentous achievements and global deliveries of GSR have been fueled by the integration of abundant genetic resources,functional gene discoveries,and innovative breeding techniques with precise gene and whole-genome selection and efficient agronomic management to promote resource-saving,environmentally friendly crop production systems.We also provide perspectives on new horizons in genomic breeding technologies geared toward delivering green and nutritious crop varieties to further enhance the development of green agricul-ture and better nourish the world population. | Sibin Yu Jauhar Ali Shaochuan Zhou Guangjun Ren Huaan Xie Jianlong Xu Xinqiao Yu Fasong Zhou Shaobing Peng Liangyong Ma Dingyang Yuan Zefu Li Dazhou Chen Ruifeng Zheng Zhigang Zhao Chengcai Chu Aiqing You Yu Wei Susong Zhu Qiongyao Gu Guangcun He Shigui Li Guifu Liu Changhua Liu Chaopu Zhang Jinghua Xiao Lijun Luo Zhikang Li Qifa Zhang | 2022 | Molecular Plant2022，15，1： | 6 |
| 5 | Molecular analysis of rice plants harboring a multi-functional T-DNA tagging system显示文摘About 25,000 rice T-DNA insertional mutant lines were generated using the vector pCAS04 which has both promoter-trapping and activation-tagging function. Southern blot analysis revealed that about 40% of these mutants were single copy integration and the average T-DNA insertion number was 2.28. By extensive phenotyping in the field, quite a number of agronomically important mutants were obtained. Histochemical GUS assay with 4,310 primary mutants revealed that the GUS-staining frequency was higher than that of the previous reports in various tissues and especially high in flowers. The T-DNA flanking sequences of some mutants were isolated and the T-DNA insertion sites were mapped to the rice genome. The flanking sequence analysis demonstrated the different integration pattern of the right border and left border into rice genome. Compared with Arabidopsis and poplar, it is much varied in the T-DNA border junctions in rice. | Yimian Ma Luo Liu Chengguang Zhu Changhui Sun Bo Xu Jun Fang Jiuyou Tang Anding Luo Shouyun Cao Gupo Li Qian Qian Yongbiao Xue Chengcai Chu | 2009 | Journal of Genetics and Genomics2009，36，5： | 3 |
| 6 | Synthesis of poly (1-oxo-2-phenyltrimethylene)catalyzed by palladium- rare earth catalyst 显示文摘 | Tian Jing Guo Jintang Zhu Chengcai | 2007 | Fibers and Polymers2007，8，6： | 1 |
| 7 | Comparing the Efficacy of a Multimodular Flexible Ureteroscope With Its Conventional Counterpart in the Management of Renal Stones显示文摘 | Jie Ding Ding Xu Qifeng Cao Tao Huang Yunkai Zhu Kai Huang Yifan Chen Chengcai Liang Jun Qi Yunteng Huang | 2015 | Urology2015，，： | 1 |
| 8 | OsCPL3 is involved in brassinosteroid signaling by regulating OsGSK2 stability显示文摘Glycogen synthase kinase 3(GSK3)proteins play key roles in brassinosteroid(BR)signaling during plant growth and development by phosphorylating various substrates.However,how GSK3 protein stability and activity are themselves modulated is not well understood.Here,we demonstrate in vitro and in vivo that C-TERMINAL DOMAIN PHOSPHATASELIKE 3(Os CPL3),a member of the RNA Pol II CTD phosphatase-like family,physically interacts with Os GSK2 in rice(Oryza sativa).Os CPL3 expression was widely detected in various tissues and organs including roots,leaves and lamina joints,and was induced by exogenous BR treatment.Os CPL3 localized to the nucleus,where it dephosphorylated Os GSK2 at the Ser-222 and Thr-284 residues to modulate its protein turnover and kinase activity,in turn affecting the degradation of BRASSINAZOLERESISTANT 1(BZR1)and BR signaling.Loss of Os CPL3 function resulted in higher Os GSK2 abundance and lower Os BZR1 levels,leading to decreased BR responsiveness and alterations in plant morphology including semi-dwarfism,leaf erectness and grain size,which are of fundamental importance to crop productivity.These results reveal a previously unrecognized role for Os CPL3 and add another layer of complexity to the tightly controlled BR signaling pathway in plants. | Luping Gong Shenghao Liao Wen Duan Yongqiang Liu Dongmei Zhu Xiaosheng Zhou Baoping Xue Chengcai Chu Yun-Kuan Liang | 2022 | Journal of Integrative Plant Biology2022，64，8： | 1 |
| 9 | Study of Lithium Exploitation from Tibetan Kyetsé Tsakha and Lungmu Tso Salines by Freezing and Mixing显示文摘1 Introduction Lithium was used widely in batteries,lubricants,aluminium smelting,ceramics,glass,polymers and other areas due to its unique electrochemical reactivity as well as other properties(Hykawy,2010).Production of lithium comes from both brine and mineral sources.The total resources of lithium estimated by the USGS in 2014 are | ZHU Chengcai DONG Meng ZENG Yun ZHU Xianling LI Wu DONG Yaping | 2014 | Acta Geologica Sinica(English Edition)2014，88，S1： | 0 |
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