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| 1 | Erianin,a novel dibenzyl compound in Dendrobium extract,inhibits lung cancer cell growth and migration via calcium/calmodulin-dependent ferroptosis显示文摘Ferroptosis,a novel form of programmed cell death,is characterized by iron-dependent lipid peroxidation and has been shown to be involved in multiple diseases,including cancer.Stimulating ferroptosis in cancer cells may be a potential strategy for cancer therapy.Therefore,ferroptosis-inducing drugs are attracting more attention for cancer treatment.Here,we showed that erianin,a natural product isolated from Dendrobium chrysotoxum Lindl,exerted its anticancer activity by inducing cell death and inhibiting cell migration in lung cancer cells.Subsequently,we demonstrated for the first time that erianin induced ferroptotic cell death in lung cancer cells,which was accompanied by ROS accumulation,lipid peroxidation,and GSH depletion.The ferroptosis inhibitors Fer-1 and Lip-1 but not Z-VAD-FMK,CQ,or necrostatin-1 rescued erianin-induced cell death,indicating that ferroptosis contributed to erianin-induced cell death.Furthermore,we demonstrated that Ca^(2+)/CaM signaling was a critical mediator of erianin-induced ferroptosis and that blockade of this signaling significantly rescued cell death induced by erianin treatment by suppressing ferroptosis.Taken together,our data suggest that the natural product erianin exerts its anticancer effects by inducing Ca^(2+)/CaMdependent ferroptosis and inhibiting cell migration,and erianin will hopefully serve as a prospective compound for lung cancer treatment. | Peng Chen Qibiao Wu Jiao Feng Lili Yan Yitian Sun Shuiping Liu Yu Xiang Mingming Zhang Ting Pan Xiaying Chen Ting Duan Lijuan Zhai Bingtao Zhai Wengang Wang Ruonan Zhang Bi Chen Xuemeng Han Yicong Li Liuxi Chen Ying Liu Xingxing Huang Ting Jin Wenzheng Zhang Hong Luo Xiaohui Chen Yongqiang Li Qiujie Li Guohua Li Qin Zhang Lvjia Zhuo Zuyi Yang Huifen Tang Tian Xie Xiaoping Ouyang Xinbing Sui | 2020 | Signal Transduction and Targeted Therapy2020，5，1： | 42 |
| 2 | Chinese deserts and sand fields in Last Glacial Maximum and Holocene Optimum显示文摘The Last Glacial Maximum (LGM, c. 26-16 ka) and the Holocene Optimum (HO, c. 9-5 ka) were characterized by cold-dry and warm-wet climates respectively in the recently geological Earth. How Chinese deserts and sand fields responded to these distinctive climatic changes is still not clear, however. To reconstruct environments of the deserts and sand fields during the LGM and HO is helpful to understand the forcing mechanisms of environment change in this arid region, and to test paleoclimatic modeling results. Through our long-term field and laboratory investigations, 400 optically stimulated luminescence (OSL) ages and more than 100 depositional records in the Chinese deserts and sand fields were obtained; on the basis of these data, we reconstruct spatial distributions of the deserts and sand fields during the LGM and HO. Our results show that the sand fields of Mu Us, Hunshandake, Horqin and Hulun Buir in northern and northeastern China had expanded 25%, 37%, 38% and 270%, respectively, during the LGM; the sand fields of Gonghe in the northeastern Qinghai-Tibetan Plateau had expanded 20%, and the deserts of Badain Jaran, Tengger in central northern China had expanded 39% and 29% separately during the LGM; the deserts of Taklimakan, Gurbantünggüt and Kumtag in northwestern China had expanded 10%-20% respectively, compared to their modern areas. On the other hand, all of the sand fields were nearly completely covered by vegetation during the HO; the deserts in northwestern and central northern China were reduced by around 5%-20% in area during this time. Lakes in this arid region were probably expanded during the HO but this conclusion needs more investigation. Compared with the geological distributions of deserts and sand fields, human activity has clearly changed (expanded) the area of active sand dunes at the present time. Our observations show that environmental conditions of Chinese deserts and sand fields are controlled by regional climate together with human activity. | LU HuaYu YI ShuangWen XU ZhiWei ZHOU YaLi ZENG Lin ZHU FangYing FENG Han DONG LiNa ZHUO HaiXin YU KaiFeng MASON Joseph WANG XiaoYong CHEN YingYong LU Qi WU Bo DONG ZhiBao QU JianJun WANG XunMing GUO ZhengTang | 2013 | Chinese Science Bulletin2013，58，23： | 40 |
| 3 | Strategies of minimally invasive treatment for intrahepatic and extrahepatic bile duct stones显示文摘 | Zongming Zhang Zhuo Liu Limin Liu Mengmeng Song Chong Zhang Hongwei Yu Baijiang Wan Mingwen Zhu Zixu Liu Hai Deng Haiming Yuan Haiyan Yang Wenping Wei Yue Zhao | 2017 | Frontiers of Medicine2017，11，4： | 37 |
| 4 | The Genome of Medicinal Plant Macleaya cordata Provides New Insights into Benzylisoquinoline Alkaloids Metabolism显示文摘在动物农业和药的抗菌素的 overuse 引起了一系列潜在的威胁到公共健康。Macleaya cordata 是从 Papaveraceae 家庭的药用的植物种,提供为抗菌剂的制造的一个安全资源为家畜喂添加剂。从 M. 的活跃成分 ? cordata 被知道包括 benzylisoquinoline 碱(偏爱) 象 sanguinarine (圣) 和 chelerythrine (CHE ) 那样的 ?,而是他们的新陈代谢的小径还得在这非模型植物被学习。在 M. 的圣和 CHE 的活跃生合成 ? cordata 被喂 13标记 C 的酷氨酸。为了增加,推进卓见,我们 de novo 定序 M 的整个染色体。cordata,第一从 Papaveraceae 家庭被定序。M.? 盖住 378 Mb 的 cordata 染色体与 43.5%being 编码 22,328 预言的编码蛋白质的基因 transposable 元素。作为基础 eudicot 的一个成员, M.? cordata 染色体缺乏 ? 发生在几乎所有 eudicots 的 paleohexaploidy 事件。从 genomics 数据, 16 的一个完全的集合 ? 新陈代谢 ? 为圣和 CHE 生合成的基因被检索,并且 14 项他们的生物化学的活动被验证。这些 genomics 和新陈代谢的数据在 M. 显示出保存 BIA 新陈代谢的小径 ? cordata 并且由庄稼改进或微生物引起的小径重建为圣和 CHE 的未来生产提供知识基础。 | Xiubin Liu Yisong Liu Peng Huang Yongshuo Ma Zhixing Qing Qi Tang Huifen Cao Pi Cheng Yajie Zheng Zejun Yuan Yuan Zhou Jinfeng Liu Zhaoshan Tang Yixiu Zhuo Yancong Zhang Linlan Yu Jialu Huang Peng Yang Qiong Peng dinbo Zhang Wenkai Jiang Zhonghua Zhang Kui Lin Dae-Kyun Ro Xiaoya Chen Xingyao Xiong Yi Shang Sanwen Huang Jianguo Zeng | 2017 | Molecular Plant2017，10，7： | 19 |
| 5 | MicroRNA-15a/b are up-regulated in response to myocardial ischemia/reperfusion injury显示文摘ObjectiveSeveral 研究显示了 miR-15a, miR-15b 和 miR-16 可以是 apoptosis 的重要管理者。后来稀释 apoptosis 能保护心肌层和还原剂梗塞尺寸,现在的学习被瞄准发现是否这些 miRNAs 参予调整心肌的局部缺血灌注( I/R )在受到 I/R 的老鼠心的 injury.MethodsApoptosis 被 TUNEL 试金在 vivo 检测,当流动 cytometry 分析由 Annexin V/PI 列在后面时,在 vitro 的两倍污点被用来在受到 hypoxia/reoxygenation ( H/R )的有教养的 cardiomyocytes 检测 apoptosis 。Taqman 即时量的 PCR 被用来证实 miR-15a/15b/16 是否涉及心脏的 I/R 的规定,控制, I/R 或 H/R 的到那些的 H/R.ResultsCompared 导致了 cardiomyocytes 的 apoptosis 显著地在 vivo 两个都被增加(24.4%±9.4% 对 2.2%±1.9% , P <0.01, n = 5 ) 并且在 vitro (14.12%±0.92% 对 2.22%±0.08%) 。 miR-15a 和 miR-15b 的表达式,然而并非 miR-16 ,在鼠标 I/R 模型,和结果被增加在 H/R model.ConclusionsOur 数据是一致的显示因此, miR-15 和 miR-15b 响应心脏的 I/R 损害是起来调整的 miR-15a/b 的下面规定可以是有希望的策略减少心脏的 I/R 损害导致的心肌的 apoptosis 。 | Li-Feng Liu Zhuo Liang Zhen-Rong Lv Xiu-Hua Liu Jing Bai Jie Chen Chen Chen Yu Wang | 2012 | Journal of Geriatric Cardiology2012，9，1： | 15 |
| 6 | Preparation and anisotropic properties of textured structural ceramics: A review显示文摘Ceramics are usually composed of randomly oriented grains and intergranular phases, so their properties are the statistical average along each direction and show isotropy corresponding to the uniform microstructures. Some methods have been developed to achieve directional grain arrangement and preferred orientation growth during ceramic preparation, and then textured ceramics with anisotropic properties are obtained. Texture microstructures give particular properties to ceramics along specific directions, which can effectively expand their application fields. In this review, typical texturing techniques suitable for ceramic materials, such as hot working, magnetic alignment, and templated grain growth(TGG), are discussed. Several typical textured structural ceramics including α-Al2O3 and related nacre bioinspired ceramics, Si3N4 and SiAlON, h-BN, MB2 matrix ultra-high temperature ceramics, MAX phases and their anisotropic properties are presented. | Zhuo ZHANG Xiaoming DUAN Baofu QIU Zhihua YANG Delong CAI Peigang HE Dechang JIA Yu ZHOU | 2019 | Journal of Advanced Ceramics2019，8，3： | 14 |
| 7 | Cardioprotective effects of Guanxinshutong (GXST) against myocardial ischemia/ reperfusion injury in rats显示文摘对 cardioprotective 药的灌注损害的 BackgroundThe 保护的效果最近被评估了并且发现了不适当。Guanxinshutong (GXST ) ,传统的植物的联合和蒙古的药,在在临床的试用对待心绞痛有效、安全。我们在老鼠对心肌的局部缺血和灌注(MI/R ) 损害估计 GXST 的 cardioprotective 效果并且探索它的可能的 mechanism.MethodsForty -- 五只男 Sprague Dawley 老鼠被使随机化进三个组:non-MI/R 组(假冒, n = 15 ) ,与车辆对待的 MI/R 组(控制, n = 15 ) 并且与 GXST 对待的 MI/R 组(药, n = 15 ) 。MI/R 被左前面的下降冠的动脉(男孩) 的结扎导致 30 分钟,在控制和药组由 2/24 小时灌注列在后面。在假冒的组,没有吸藏, LAD 被暴露。GXST 粉末(在药组) 或盐(在控制和假冒的组) 在外科以前从 7 白天经由直接胃的 gavage 被管理。血样品从颈动脉动脉被收集(10 只老鼠各组织) 在 2 小时灌注以后,决定肿瘤坏死 factor-α 的层次;(TNF-α) , interleukin-1β(IL-1β) , interleukin-6 (IL-6 ) 和用连接酶的 immunosorbent 试金的细胞间的粘附 molecule-1 (ICAM-1 ) 。动物然后被牺牲,心为组织病理学说和西方的污点分析被收获。梗塞尺寸在留下被测量在在 24 小时 reperfusion.ResultsGXST 以后的每个组的五只老鼠显著地减少了 TNF-α 的层次;, IL-1β, IL-6, ICAM-1, apoptosis 索引(AI ) 和梗塞缩放。GXST 显然也禁止了原子因素 kappa B (NF-κ B ) 活动什么时候与控制组相比(所有 P <0.05 ).ConclusionsGXST 在在老鼠保护心肌层免于 MI/R 损害是有效的。它的可能的 cardioprotective 机制包含煽动性的反应和 apoptosis 追随者 MI/R 损害的抑制。 | Zhuo Llang Li-Feng Llu Tian-Ming Yao Yu Huo Ya-Ling Han | 2012 | Journal of Geriatric Cardiology2012，9，2： | 13 |
| 8 | Dual-targeting and microenvironment-responsive micelles as a gene delivery system to improve the sensitivity of glioma to radiotherapy显示文摘Dbait is a small double-stranded DNA molecule that has been utilized as a radiosensitizer to enhance the sensitivity of glioma to radiotherapy(RT). However, there is no effective drug delivery system to effectively overcome the blood–brain barrier(BBB). The aim of this study was to develop a gene delivery system by using the BBB and glioma dual-targeting and microenvironment-responsive micelles(ch-Kn(s-s)R8-An) to deliver Dbait into glioma for RT. Angiopep-2 can target the low-density lipoprotein receptor-related protein-1(LRP1) that is overexpressed on brain capillary endothelial cells(BCECs) and glioma cells. In particular, due to upregulated matrix metalloproteinase 2(MMP-2) in the tumor microenvironment, we utilized MMP-2-responsive peptides as the enzymatically degradable linkers to conjugate angiopep-2. The results showed that ch-Kn(s-s)R8-An micelles maintained a reasonable size(80–160 nm) with a moderate distribution and a decreased mean diameter from the cross-linking as well as exhibited low critical micelle concentration(CMC) with positive surface charge, ranging from 15 to40 mV. The ch-K5(s-s)R8-An/pEGFP showed high gene transfection efficiency in vitro, improved uptake in glioma cells and good biocompatibility in vitro and in vivo. In addition, the combination of ch-K5(s-s)R8-An/Dbait with RT significantly inhibited the growth of U251 cells in vitro. Thus, ch-K5(s-s)R8-An/Dbait may prove to be a promising gene delivery system to target glioma and enhance the efficacy of RT on U251 cells. | Xiuxiu Jiao Yuan Yu Jianxia Meng Mei He Charles Jian Zhang Wenqian Geng Baoyue Ding Zhuo Wang Xueying Ding | 2019 | Acta Pharmaceutica Sinica B2019，9，2： | 13 |
| 9 | Structural insights into the TRIM family of ubiquitin E3 ligases显示文摘 | Yang Li Han Wu Wei Wu Wei Zhuo Weixiao Liu Yixiao Zhang Minzhang Cheng Ye-Guang Chen Ning Gao Hongtao Yu Linfang Wang Wei Li Maojun Yang | 2014 | Cell Research2014，24，6： | 12 |
| 10 | A^200 ka pollen record from Okinawa Trough:Paleoenvironment reconstruction of glacial-interglacial cycles显示文摘Pollen analysis was carried out on the Core MD982194 of 29.78 m retrieved from the Middle Okinawa Trough which was dated as old as^200 ka BP.The results revealed that pollen assemblages mainly presented an alternation of coniferous and herb pollen.The coniferous saccate pollen,principally Pinus and Tsuga,predominated in most parts of the core,especially highlighted in the interstadial stages including MIS 1,3,5 and MIS7,whereas the herb pollen significantly increased in the glacial periods.Thus the pollen flora and their percentages showed the sensitive changes under the influence of ice volume during the glacial and interglacial periods.Our record from this core has first documented that the percentage of Cyperaceae was extremely high in the glacial stages with a notable increase in Artemisia,Gramineae,Asteraceae,Chenopodiaceae,and freshwater algae,which can be used as a proxy for sea-level change at the study site because of their close negative correlation of the orbital-scale changes in sea level.The distance between the continental coastline and the Okinawa Trough has deeply shortened due to the sea-level drop in the LGM.As a result,the sediment materials from Yangtze River were extensively deposited on the flat,exposed continental shelf owing to the rapid decline of river flow speed,leading to that pollen grains from Okinawa Trough are derived mainly from the flat coastal vegetation of exposed continental shelf at glacial stages.Changes of pollen assemblage were consistent with the variation of temperature and humidity,which showed that the percentage of arboreal pollen was highly augmented at MIS 7,5 and MIS 1,corresponding to the strengthening of the East Asian summer monsoon and increasing of rainfall.Moreover,the peak of Pinus percentage in MIS 5.3,5.1 and MIS 3.3 may be closely linked by orbital and sub-orbital cycles of solar radiation and monsoonal variability.The present study of core MD981294 implied lower temperature and precipitation during the lowest sea-level stage(LGM),and more visibly testified that the vegetation of the flat plain on the exposed continental shelf was dominated by intrazonal communities such as halophyte grasslands and freshwater wetlands instead of zonal steppe or semi-arid desert.All above evidence demonstrated that the fundamental changes of pollen assemblage and their origins in Okinawa Trough since^200 ka BP were affected by combine factors including the coastline position and climate fluctuation.Moreover,the substantial shortening of distance between shoreline and the Okinawa Trough driven by orbital insolation cycles was clearly indicated by the pollen spectra,whereas the source-area climate signal of the pollen record was largely weakened. | ZHENG Zhuo HUANG KangYou DENG Yun CAO LingLong YU ShaoHua Jean-Pierre SUC Serge BERNE Franois GUICHARD | 2013 | Science China Earth Sciences2013，56，10： | 11 |
| 11 | Current Status of Surgical Treatment of Biliary Diseases in Elderly Patients in China显示文摘 | Zong-Ming Zhang Jia-Hong Dong Fang-Cai Lin Qiu-Sheng Wang Zhi Xu Xiao-Dong He Chong Zhang Zhuo Liu Li-Min Liu Hai Deng Hong-Wei Yu Bai-Jiang Wan Ming-Wen-Zhu Hai-Yan Yang Meng-Meng Song Yue Zhao | 2018 | Chinese Medical Journal2018，，15： | 11 |
| 12 | Bedaquiline-containing regimens in patients with pulmonary multidrug-resistant tuberculosis in China:focus on the safety显示文摘Background:World Health Organization recommends countries introducing new drug and short treatment regimen for drug resistant tuberculosis(DR-TB)should develop and implement a system for active pharmacovigilance that allows for detection,reporting and management of adverse events.The aim of the study is to evaluate the frequency and severity of adverse events(AEs)of bedaquiline-containing regimen in a cohort of Chinese patients with multidrug-resistant(MDR)/extensively drug-resistant(XDR)-TB based on active drug safety monitoring(aDSM)system of New Drug Introduction and Protection Program(NDIP). | Jing-Tao Gao Juan Du Gui-Hui Wu Yi Pei Meng-Qiu Gao Leonardo Martinez Lin Fan Wei Chen Li Xie Yu Chen Hua Wang Long Jin Guo-Bao Li Pei-Lan Zong Yu Xiong Qian-Hong Wu Ming-Wu Li Xiao-Feng Yan Yan-Fang Miao Qing-Shan Cai Xin-Jie Li Da-Peng Bai Shu-Jun Geng Guo-Li Yang Pei-JunTang Yi Zeng Xiao-Hong Chen Tong-Xia Li Cui Cai Yun Zhou Ma Zhuo Jian-Yun Wang Wen-Long Guan Lin Xu Ji-Chan Shi Wei Shu Li-Li Cheng FeiTeng Yu-Jia Ning Shi-Heng Xie Yu-Xian Sun Li-Jie Zhang Yu-Hong Liu | 2021 | Infectious Diseases of Poverty2021，10，2： | 11 |
| 13 | Asymmetric Dimethylarginine Predicts One-year Recurrent Cardiovascular Events: Potential Biomarker of 'Toxin Syndrome' in Coronary Heart Disease显示文摘Objective: To examine the prognostic value of serum levels of asymmetric dimethylarginine(ADMA)in patients with stable coronary heart disease(CHD) thus explore a potential biomarker of 'toxin syndrome' in CHD.Methods: In this prospective nested case-control study, 36 of 1,503 Chinese patients with stable CHD experienced at least 1 recurrent cardiovascular event(RCE) during 1-year fol ow-up. Serum levels of ADMA at the start of fol ow-up were compared between these 36 cases and 36 controls which matched to cases in terms of gender, age, history of hypertension, and myocardial infarction. Results: Based on the crude model, subjects in the 2 highest ADMA quartiles showed signi?cantly higher risk of developing RCE than those in the lowest ADMA quartile [odds ratio(OR) 4.09, 95%confidence interval(CI) 1.01 to 16.58; OR 6.76, 95% CI 1.57 to 29.07]. This association was also observed in the case-mix model(OR 5.51, 95% CI 1.23 to 24.61; OR 7.83, 95% CI 1.68 to 36.41) and multivariable model(OR 6.64,95% CI 1.40 to 31.49; OR 13.14, 95% CI 2.28 to 75.71) after adjusting for confounders. The multivariable model which combined ADMA and high-sensitivity C-reactive protein(hs CRP) showed better predictive power with areas under the receiver operator characteristic curves(0.779) than the model of either ADMA(0.694) or hs CRP(0.636). Conclusion:Serum ADMA level may be a potential biomarker of 'toxin syndrome' in CHD which shows favorable prognostic value in predicting 1-year RCE in patients with stable CHD. [The registration number is Chi CTR-PRNRC-07000012] | XU Hao CHEN Zhuo SHANG Qing-hua GAO Zhu-ye YU Chang-an SHI Da-zhuo CHEN Ke-ji | 2019 | Chinese Journal of Integrative Medicine2019，25，5： | 10 |
| 14 | 晚期非小细胞肺癌免疫检查点抑制剂新型生物标志物:血液PD-L1动态检测显示文摘背景免疫检查点抑制剂(immune checkpoint inhibitors,ICIs)具有良好疗效已成为恶性肿瘤治疗领域备受瞩目的翘楚。然而,仅有小部分患者获得长期临床获益。如何选择生物标志物来筛查ICIs治疗优势人群仍是一大热点。方法收集51例晚期恶性肿瘤患者的肿瘤组织和配对血样本,进行PD-L1表达的相关性分析。40例晚期非小细胞肺癌(non-small cell lung cancer,NSCLC)患者接受ICIs治疗,2个月时检测血液PD-L1(bloodPD-L1,bPD-L1)表达的动态变化,包括PD-L1 mRNA、外泌体PD-L1(exosomalPD-L1,exoPD-L1)蛋白和可溶性PD-L1(solublePD-L1,sPD-L1)。应用R软件计算最佳截断值,用于预测最佳客观缓解(best objective response,b OR)、无进展生存期(progression-free survival,PFS)和总生存期(overall survival,OS)。结果51例不同恶性肿瘤患者中,组织PD-L1(tissuePD-L1,tPD-L1)表达阳性患者的PD-L1mRNA表达水平显著升高。40例接受ICIs治疗的晚期NSCLC患者中,bPD-L1 mRNA上调≥2.04的患者b OR、PFS和OS更佳;21例接受ICIs治疗的晚期NSCLC患者中,exoPD-L1上调≥1.86的患者临床获益更大。sPD-L1动态变化与ICIs疗效无显著相关性。PD-L1 mRNA和exoPD-L1联合检测更有利于预测ICIs治疗疗效。结论bPD-L1表达与tPD-L1表达存在正相关;晚期NSCLC患者ICIs治疗初期PD-L1 mRNA和(或)exoPD-L1表达升高,可作为评估临床获益的新型生物标志物。 | Qiao YANG Mingjing CHEN Jiaoyang GU Kai NIU Xianlan ZHAO Linpeng ZHENG Zihan XU Yongxin YU Feng LI Lingxin MENG Zhengtang CHEN Wenlei ZHUO Luping ZHANG Jianguo SUN 杨峤(译) 孙建国(译) | 2021 | 中国肺癌杂志2021，24，12： | 10 |
| 15 | Dendritic cell-based vaccination with lentiviral vectors encoding ubiquitinated hepatitis B core antigen enhances hepatitis B virus-specific immune responses in vivo显示文摘肝炎 B 病毒(HBV ) 的活动特定的细胞毒素的 T 淋巴细胞(CTL ) 戏在 HBV 的清理的一个占优势的角色。树枝状的房间(DC ) 是关键介绍抗原的房间并且在有免疫力的回答的开始起一个重要作用。我们以前验证了有效地编码 ubiquitinated 肝炎 B 核心抗原(LV-Ub-HBcAg ) 的那 lentiviral 向量导致成熟的 transduced DC,和成熟 DC 高效地导致了 T 房间极化到 Th1 并且产生了 HBcAg 特定的 CTL 前 vivo。在这研究,在 BALB/c 老鼠的 LV-Ub-HBcAg 的 HBV 特定的有免疫力的回答(H-2K d ) 被评估。LV-Ub-HBcAg 的直接注射增加了 cytokines IL-2 和 IFN- 的生产,这被显示出,得到了强壮的抗体回答,并且显著地产生了 IFN-+ 有在 BALB/c 老鼠的 HBV 特定的 CTL 回答的 CD8 + T 房间。另外, LV-Ub-HBcAg 的直接注射导致了有势力 anti-HBV 免疫者回答,类似于得到由的那些在 vitro-transduced DC。在结论,基于 DC 的治疗学的疫苗的 LV-Ub-HBcAg 得到了特定的抗体免疫者回答并且在 vivo 导致了柔韧的特定的 CTL 活动。 | Shenglan Dai Meng Zhuo Linlin Song Xiaohua Chen Yongsheng Yu Zhenghao Tang Guoqing Zang | 2015 | Acta Biochimica et Biophysica Sinica2015，47，11： | 9 |
| 16 | Attenuated Listeria monocytogenes as a cancer vaccine vector for the delivery of CD24, a biomarker for hepatic cancer stem cells显示文摘稀释 Listeria monocytogenes (LM ) 是为癌症疫苗的交货的有希望的候选人向量。在由介绍抗原的房间的吞噬作用以后,这个细菌刺激主要 histocompatibility 建筑群(MHC ) 我和 MHC-II 小径并且导致抗原特定的 T 淋巴细胞的增长。包含复制缺乏的 LM 种类 Δ 的基因修正的新策略; dalΔ dat (Lmdd ) 被开发了表示并且藏匿人的 CD24 蛋白质。CD24 是仔细与 hepatocellular 癌(HCC ) 的 apoptosis,转移和复发被联系的肝的癌症干细胞 biomarker。在在老鼠的静脉内的管理以后, Lmdd-CD24 首先在怒气和肝被散布并且没引起严重机关损害。Lmdd-CD24 有效地增加了干扰素(IFN ) 的数字 -γ-producing CD8 + T 房间和 IFN-γ分泌物。Lmdd-CD24 也提高了 IL-4- 和 IL-10-producing T 助手 2 房间的数字。Lmdd-CD24 疫苗的功效进一步对 Hepa1-6-CD24 肿瘤被调查,它腹股沟地被接种进老鼠。Lmdd-CD24 显著地在老鼠减少了肿瘤尺寸并且增加了他们的幸存。尤其是, T 规章的房间(Treg ) 的减小数并且特定的 CD8 +T 房间活动的改进在渗入肿瘤的淋巴细胞(TIL ) 被观察。这些结果对 HCC 建议 Lmdd-CD24 疫苗的一个潜在的应用程序。 | Yu Yang Jiajie Hou Zhe Lin Han Zhuo DianyuChen Xudong Zhang Yun Chen Beicheng Sun | 2014 | Cellular & Molecular Immunology2014，11，2： | 9 |
| 17 | Analysis of genetic architecture and favorable allele usage of agronomic traits in a large collection of Chinese rice accessions显示文摘Genotyping and phenotyping large natural populations provide opportunities for population genomic analysis and genome-wide association studies(GWAS). Several rice populations have been re-sequenced in the past decade;however, many major Chinese rice cultivars were not included in these studies. Here, we report large-scale genomic and phenotypic datasets for a collection mainly comprised of 1,275 rice accessions of widely planted cultivars and parental hybrid rice lines from China. The population was divided into three indica/Xian and three japonica/Geng phylogenetic subgroups that correlate strongly with their geographic or breeding origins. We acquired a total of 146 phenotypic datasets for 29 agronomic traits under multi-environments for different subpopulations. With GWAS, we identified a total of 143 significant association loci, including three newly identified candidate genes or alleles that control heading date or amylose content. Our genotypic analysis of agronomically important genes in the population revealed that many favorable alleles are underused in elite accessions, suggesting they may be used to provide improvements in future breeding efforts. Our study provides useful resources for rice genetics research and breeding. | Xiuxiu Li Zhuo Chen Guomin Zhang Hongwei Lu Peng Qin Ming Qi Ying Yu Bingke Jiao Xianfeng Zhao Qiang Gao Hao Wang Yunyu Wu Juntao Ma Liyan Zhang Yongli Wang Lingwei Deng Shanguo Yao Zhukuang Cheng Diqiu Yu Lihuang Zhu Yongbiao Xue Chengcai Chu Aihong Li Shigui Li Chengzhi Liang | 2020 | Science China(Life Sciences)2020，63，11： | 8 |
| 18 | Electroacupuncture pretreatment exhibits antidepressive effects by regulating hippocampal proteomics in rats with chronic restraint stress显示文摘The clinical effect of electroacupuncture on depression is widely recognized. However, the signal transduction pathways and target proteins involved remain unclear. In the present study, rat models of chronic restraint stress were used to explore the mechanism by which electroacupuncture alleviates depression. Rats were randomly divided into control, model, and electroacupuncture groups. Chronic restraint stress was induced in the model and electroacupuncture groups by restraining rats for 28 days. In the electroacupuncture group, electroacupuncture pretreatment at Baihui(GV20) and Yintang(GV29) acupoints was performed daily(1 m A, 2 Hz, discontinuous wave, 20 minutes) prior to restraint for 28 days. Open field tests and body weight measurements were carried out to evaluate the depressive symptoms at specific time points. On day 28, the crossing number, rearing number, and body weights of the model group were significantly lower than those in the control group. Behavior test results indicated that rat models of depressive-like symptoms were successfully established by chronic restraint stress combined with solitary raising. On day 28, an isobaric tag for a relative and absolute quantitation-based quantitative proteomic approach was performed to identify differentially expressed proteins in hippocampal samples obtained from the model and electroacupuncture groups. The potential function of these differential proteins was predicted through the use of the Cluster of Orthologous Groups of proteins(COG) database. Twenty-seven differential proteins(uncharacteristic proteins expected) were selected from the model and electroacupuncture groups. In addition to unknown protein functions, COG are mainly concentrated in general prediction function, mechanism of signal transduction, amino acid transport and metabolism groups. This suggests that electroacupuncture improved depressive-like symptoms by regulating differential proteins, and most of these related proteins exist in nerve cells. | Zhuo Guo Ya Tu Tian-wei Guo Yun-chu Wu Xue-qin Yang Lan Sun Xin-jing Yang Wen-yue Zhang Yu Wang Xu-hui Zhang | 2015 | Neural Regeneration Research2015，10，8： | 8 |
| 19 | A cyclodextrin-based nanoformulation achieves co-delivery of ginsenoside Rg3 and quercetin for chemo-immunotherapy in colorectal cancer显示文摘The immune checkpoint blockade therapy has profoundly revolutionized the field of cancer immunotherapy. However, despite great promise for a variety of cancers, the efficacy of immune checkpoint inhibitors is still low in colorectal cancer(CRC). This is mainly due to the immunosuppressive feature of the tumor microenvironment(TME). Emerging evidence reveals that certain chemotherapeutic drugs induce immunogenic cell death(ICD), demonstrating great potential for remodeling the immunosuppressive TME. In this study, the potential of ginsenoside Rg3(Rg3) as an ICD inducer against CRC cells was confirmed using in vitro and in vivo experimental approaches. The ICD efficacy of Rg3 could be significantly enhanced by quercetin(QTN) that elicited reactive oxygen species(ROS). To amelioratein vivo delivery barriers associated with chemotherapeutic drugs, a folate(FA)-targeted polyethylene glycol(PEG)-modified amphiphilic cyclodextrin nanoparticle(NP) was developed for co-encapsulation of Rg3 and QTN. The resultant nanoformulation(CD-PEG-FA.Rg3.QTN) significantly prolonged blood circulation and enhanced tumor targeting in an orthotopic CRC mouse model, resulting in the conversion of immunosuppressive TME. Furthermore, the CD-PEG-FA.Rg3.QTN achieved significantly longer survival of animals in combination with Anti-PD-L1. The study provides a promising strategy for the treatment of CRC. | Dandan Sun Yifang Zou Liu Song Shulan Han Hao Yang Di Chu Yun Dai Jie Ma Caitriona M.O’Driscoll Zhuo Yu Jianfeng Guo | 2022 | Acta Pharmaceutica Sinica B2022，12，1： | 8 |
| 20 | A Quantitative Evaluation of Shale Gas Content in Different Occurrence States of the Longmaxi Formation: A New Insight from Well JY-A in the Fuling Shale Gas Field,Sichuan Basin显示文摘Comprehensive quantitative evaluation of shale gas content and the controlling factors in different occurrence states is of great significance for accurately assessing gas-bearing capacity and providing effective well-production strategies. A total of 122 core samples from well JY-A in the Fuling shale gas field were studied to reveal the characteristics of S_1 l shale,15 of which were selected to further predict the shale gas content in different occurrence states, which are dependent on geological factors in the thermal evolution process. Geological parameters were researched by a number of laboratory programs, and the factors influential in controlling shale gas content were extracted by both PCA and GRA methods and prediction models were confirmed by the BE method using SPSS software. Results reveal that the adsorbed gas content is mainly controlled by TOC, Ro, SSA, PD and pyrite content, and the free gas content is mainly controlled by S_2, quartz content, gas saturation and formation pressure for S_1 l in well JY-A. Three methods, including the on-site gas desorption method, the empirical formula method, and the multiple regression analysis method were used in combination to evaluate the shale gas capacity of well JY-A, all of which show that the overall shale gas content of well JY-A is in the range of 2.0–5.0 m^3/t and that the free gas ratio is about 50%, lower than that of well JY-1. Cause analysis further confirms the tectonics and preservation conditions of S_1 l in the geological processes, especially the influence of eastern boundary faults on well JY-A, as the fundamental reasons for the differences in shale gas enrichment in the Jiaoshiba area. | TANG Ling SONG Yan LI Qianwen PANG Xiongqi JIANG Zhenxue LI Zhuo TANG Xianglu YU Hailong SUN Yue FAN Shichao ZHU Lin | 2019 | Acta Geologica Sinica(English Edition)2019，93，2： | 7 |
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