|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Chinese Stroke Association guidelines for clinical management of cerebrovascular disorders:executive summary and 2019 update of clinical management of stroke rehabilitation显示文摘Stroke is the third leading cause of long-term disability.Effective stroke rehabilitation should be provided to patients with a moderate or severe stroke so as to achieve functional recovery.Also,stroke rehabilitation is moving towards more intense therapy models that incorporate technologies such as robotics and computer games.Evidence-based guidelines are useful for promoting the best possible clinical practices.This guideline summarises the modes of stroke rehabilitation in China-three-stage rehabilitation network,rehabilitation of stroke dysfunction,training for activities of daily living,stroke complications and rehabilitation care.The aim of this guideline was to provide a synopsis of best clinical practices in the stroke rehabilitation of adult patients in China. | Tong Zhang Jun Zhao Xueping Li Yulong Bai Baojun Wang Yun Qu Bingjie Li Shengjie Zhao Chinese Stroke Association Stroke Council Guideline Writing Committee | 2020 | Stroke & Vascular Neurology2020，5，3： | 38 |
| 2 | A practical guide to amplicon and metagenomic analysis of microbiome data显示文摘Advances in high-throughput sequencing(HTS)have fostered rapid developments in the field of microbiome research,and massive microbiome datasets are now being generated.However,the diversity of software tools and the complexity of analysis pipelines make it difficult to access this field.Here,we systematically summarize the advantages and limitations of microbiome methods.Then,we recommend specific pipelines for amplicon and metagenomic analyses,and describe commonly-used software and databases,to help researchers select the appropriate tools.Furthermore,we introduce statistical and visualization methods suitable for microbiome analysis,including alpha-and betadiversity,taxonomic composition,difference comparisons,correlation,networks,machine learning,evolution,source tracing,and common visualization styles to help researchers make informed choices.Finally,a stepby-step reproducible analysis guide is introduced.We hope this review will allow researchers to carry out data analysis more effectively and to quickly select the appropriate tools in order to efficiently mine the biological significance behind the data. | Yong-Xin Liu Yuan Qin Tong Chen Meiping Lu Xubo Qian Xiaoxuan Guo Yang Bai | 2021 | Protein & Cell2021，12，5： | 20 |
| 3 | Nickel-exposed Workers in China: A Cohort Study显示文摘There are more than 50 000 workers in Jinchuan Group Co,Ltd(JNMC).Since all staff in JNMC are eligible for a medical examination every two years,only 23 484 nickel-exposed subjects who participated in medical examination were included in this study.Their data,collected from June 22,2011 to September 28,2012,in a | BAI Ya Na YAGN Ai Min PU Hong Quan HE Jie CHENG Ning ZHENG Tong Zhang DAI Min ZHANG Ya Wei BASSING Bryan WANG Qiu Ying | 2014 | Biomedical and Environmental Sciences2014，27，3： | 20 |
| 4 | Methylation of Dickkopf-3 as a prognostic factor in cirrhosis-related hepatocellular carcinoma显示文摘AIM:To investigate the prevalence and time of Dickkopf(DKK) family methylation and its clinical significance in hepatocarcinogenesis. METHODS:Methylation of DKK family genes was quantitatively analyzed in 115 liver tissue samples,including 50 pairs of primary hepatocellular carcinoma(HCC) and matched noncancerous cirrhotic tissue samples,as well as 15 liver cirrhosis biopsy samples. RESULTS:The methylation level of DKK3 was significantly higher in HCC tissue samples than in matched noncancerous cirrhotic tissue samples(P<0.0001) orin liver cirrhosis biopsy samples(P=0.0139) .Receiver operator characteristic curve analysis confirmed that the percent of methylated reference(PMR) values of DKK3 could effectively discriminate HCC tissue samples from noncancerous tissue samples(AUC=0.8146) or liver cirrhosis biopsy samples(AUC=0.7093) .Kaplan-Meier survival curves revealed that the progression-free survival time of patients with a higher DKK3 methylation level(PMR >1%) was significantly shorter than that of those with a lower DKK3 methylation level(PMR≤1%)(P=0.0255) . Multivariate Cox analysis indicated that methylated DKK3 was significantly and independently related with a shorter survival time(relative risk=2.527,95%CI:1.063-6.008,P=0.036) of HCC patients. CONCLUSION:Methylation of DKK3 is an important event in early malignant transformation and HCC progression,and therefore might be a prognostic indicator for risk assessment of HCC. | Yang, Bin Du, Zhi Gao, Ying-Tang Lou, Cheng Zhang, Shi-Guang Bai, Tong Wang, Yi-Jun Song, Wen-Qin | 2010 | World Journal of Gastroenterology2010，16，6： | 16 |
| 5 | Prognosis-related classifi cation and dynamic monitoring of immune status in patients with sepsis:A prospective observational study显示文摘BACKGROUND:The dynamic monitoring of immune status is crucial to the precise and individualized treatment of sepsis.In this study,we aim to introduce a model to describe and monitor the immune status of sepsis and to explore its prognostic value.METHODS:A prospective observational study was carried out in Zhongshan Hospital,Fudan University,enrolling septic patients admitted between July 2016 and December 2018.Blood samples were collected at days 1 and 3.Serum cytokine levels(e.g.,tumor necrosis factor-α[TNF-α],interleukin-10[IL-10])and CD14+monocyte human leukocyte antigen-D-related(HLA-DR)expression were measured to serve as immune markers.Classifi cation of each immune status,namely systemic inflammatory response syndrome(SIRS),compensatory anti-inflammatory response syndrome(CARS),and mixed antagonistic response syndrome(MARS),was defined based on levels of immune markers.Changes of immune status were classifi ed into four groups which were stabilization(SB),deterioration(DT),remission(RM),and non-remission(NR).RESULTS:A total of 174 septic patients were enrolled including 50 non-survivors.Multivariate analysis discovered that IL-10 and HLA-DR expression levels at day 3 were independent prognostic factors.Patients with MARS had the highest mortality rate.Immune status of 46.1%patients changed from day 1 to day 3.Among four groups of immune status changes,DT had the highest mortality rate,followed by NR,RM,and SB with mortality rates of 64.7%,42.9%,and 11.2%,respectively.CONCLUSIONS:Severe immune disorder defi ned as MARS or deterioration of immune status defi ned as DT lead to the worst outcomes.The preliminary model of the classifi cation and dynamic monitoring of immune status based on immune markers has prognostic values and is worthy of further investigation. | Jun Yin Yao Chen Jun-ling Huang Lei Yan Zhong-shu Kuang Ming-ming Xue Si Sun Hao Xiang Yan-yan Hu Zhi-min Dong Chao-yang Tong Chun-xue Bai Zhen-ju Song | 2021 | World Journal of Emergency Medicine2021，12，3： | 16 |
| 6 | A mussel-inspired supramolecular hydrogel with robust tissue anchor for rapid hemostasis of arterial and visceral bleedings显示文摘In recent years,the developed hemostatic technologies are still difficult to be applied to the hemostasis of massive arterial and visceral hemorrhage,owing to their weak hemostatic function,inferior wet tissue adhesion,and low mechanical properties.Herein,a mussel-inspired supramolecular interaction-cross-linked hydrogel with robust mechanical property(308.47±29.20 kPa)and excellent hemostatic efficiency(96.5%±2.1%)was constructed as a hemostatic sealant.Typically,we combined chitosan(CS)with silk fibroin(SF)by cross-linking them through tannic acid(TA)to maintain the structural stability of the hydrogel,especially for wet tissue adhesion ability(shear adhesive strength=29.66±0.36 kPa).Compared with other materials reported previously,the obtained CS/TA/SF hydrogel yielded a lower amount of blood loss and shorter time to hemostasis in various arterial and visceral bleeding models,which could be ascribed to the synergistic effect of wound closure under wet state as well as intrinsic hemostatic activity of CS.As a superior hemostatic sealant,the unique hydrogel proposed in this work can be exploited to offer significant advantages in the acute wound and massive hemorrhage with the restrictive access of therapeutic moieties. | Ziwen Qiao Xueli Lv Shaohua He Shumeng Bai Xiaochen Liu Linxi Hou Jingjing He Dongmei Tong Renjie Ruan Jin Zhang Jianxun Ding Huanghao Yang | 2021 | Bioactive Materials2021，6，9： | 14 |
| 7 | Hepatitis C virus in human B lymphocytes transformed by Epstein-Barr virus in vitro by in situ reverse transcriptase-polymerase chain reaction显示文摘AIM To study persistence and replication ofheltitis C virus (HCV) in patients' peripheralblood mononuclear cells (PBMC) cultured invitro.METHODS Epstein-Barr virus (EBV) was usedto transform the hepatitis C virus from a HCVpositive patient to permanent lymphoblastoidcell lines (LCL). Positive and negative HCV RNAstrands of the cultured cells and growth mediawere detected by reverse transcriptase-polymerase chain reaction ( RT-PCR ) eachmonth. Core and NS5 proteins of HCV werefurther tested using immunohistochemical SPmethod and in situ RT-PCR.RESULTS HCV RNA positive strands wereconsistently detected the cultured cells for oneyear. The negative-strand RNA in LCL cells andthe positive-strand RNA in supernatants wereobserved intermittently. Immunohistochemicalresults medicated expression of HCV NS3 and Cproteins in LCL cytoplasm mostly. The positivesignal of PCR product was dark blue and mainlylocalized to the LCL cytoplasm. The RT-PCRsignal was eliminated by overnight RNasedigestion but not DNase digestion.CONCLUSION HCV may exist and remainfunctional in a cultured cell line for a longperiod. | Ji Lin Cheng Bao Ling Liu Yi Zhang Wen Bin Tong Zheng Yan Bai Fang Feng Institute of Hepatology,Peoples Hospital,Medical Center of Beijing University,Beijing 10(X)44,China | 2001 | World Journal of Gastroenterology2001，7，3： | 11 |
| 8 | Cyclostratigraphy of the Induan (Early Triassic) in West Pingdingshan Section,Chaohu,Anhui Province显示文摘The Induan sequence in the West Pingdingshan Section, Chaohu, Anhui Province, displays a series of superimposed mudstone/limestone cycles. The lithological character of the cycles, combined with power spectral and wavelet analysis of magnetic susceptibility readings, reveals 12 short eccentricity and 56 precession Milankovitch cycles - obliquity cycles are not apparent. The uniformity of cycle thicknesses indicates a stable depositional setting making this section ideal to perform various geo-logical studies. Accordingly, the Induan Stage is estimated to have lasted 1.1 Ma, and the depositional rate for this part of the section is about 3.7 cm/ka. This places the Induan-Olenekian boundary in the West Pingdingshan Section at about 251.5 Ma based on an age of 252.6 Ma for the Permian-Triassic boundary. | GUO Gang TONG JinNan ZHANG ShiHong ZHANG Jie BAI LingYan | 2008 | Science China Earth Sciences2008，51，1： | 10 |
| 9 | Surface modification of small intestine submucosa in tissue engineering显示文摘With the development of tissue engineering,the required biomaterials need to have the ability to promote cell adhesion and proliferation in vitro and in vivo.Especially,surface modification of the scaffold material has a great influence on biocompatibility and functionality of materials.The small intestine submucosa(SIS)is an extracellular matrix isolated from the submucosal layer of porcine jejunum,which has good tissue mechanical properties and regenerative activity,and is suitable for cell adhesion,proliferation and differentiation.In recent years,SIS is widely used in different areas of tissue reconstruction,such as blood vessels,bone,cartilage,bladder and ureter,etc.This paper discusses the main methods for surface modification of SIS to improve and optimize the performance of SIS bioscaffolds,including functional group bonding,protein adsorption,mineral coating,topography and formatting modification and drug combination.In addition,the reasonable combination of these methods also offers great improvement on SIS surface modification.This article makes a shallow review of the surface modification of SIS and its application in tissue engineering. | Pan Zhao Xiang Li Qin Fang Fanglin Wang Qiang Ao Xiaohong Wang Xiaohong Tian Hao Tong Shuling Bai Jun Fan | 2020 | Regenerative Biomaterials2020，7，4： | 9 |
| 10 | Protection of lung function by introducing single photon emission computed tomography lung perfusion image into radiotherapy plan of lung cancer显示文摘背景肺功能的地位能在肺灌注图象上被显示。与图象,肺癌症的放射疗法计划能被指导到优化的更多。这研究试图估计份量上把功能的肺成像合并到 3-dimensional 的影响保角的放射疗法(3DCRT ) 和为非小的房间肺癌症(NSCLC ) 计划 .Methods 的调制紧张的放射治疗(IMRT ) 经历了放射疗法的有 NSCLC 的十个病人在这研究被包括。在放射疗法前,每个病人与单个光子排放经历了 CT 模拟和肺灌注成像计算断层摄影术(SPECT ) 。SPECT 图象与计划 CT 的模拟被登记并且使用了功能的肺(lung-F ) 和非功能的肺(lung-NF ) 到轮廓。二个 3DCRT 计划和二个 IMRT 计划在每 patient:two 被设计并且比较独自使用模拟 CT 的解剖计划和用除了计划的四种类型的模拟 CT.Dosimetric 参数的 SPECT-CT 的二个功能的计划以肿瘤范围和正常纸巾的回避被比较。全部的放射剂量被放在 66 Gy (2 Gy ? 椠 ???? 散汬氠湩獥愠摮㈠祝栠浵湡朠獡牴捩琠獩?獥 ???? 猠数楣敭獮愠摮琠敨爠獥数瑣癩? 摡慪散瑮渠牯慭? 楴獳敵 ? 愠摮 ?? | YIN Yong CHEN Jin-hu LI Bao-sheng LIU Tong-hai LU jie BAI Tong DONG Xiao-ling YU Jin-ming | 2009 | Chinese Medical Journal2009，，5： | 8 |
| 11 | The Huashan risk calculators performed better in prediction of prostate cancer in Chinese population: a training study followed by a validation study显示文摘前列腺癌症预防试用(PCPT ) 的表演冒险计算器和其它风险计算器因为在中国人口的前列腺癌症(PCa ) 预言糟糕被理解。我们执行了这研究基于中国人口造风险计算器(Huashan 风险计算器) 并且验证了前列腺特定的抗原(PSA ) 的性能, PCPT 风险计算器,和在一个确认队的 Huashan 风险计算器。我们从在一个训练的队从 2006 年 1 月经历了起始的前列腺活体检视到 2010 年 12 月的 1059 个人基于数据造了 Huashan 风险计算器。然后,我们从 2011 年 1 月在观察确认研究验证了 PSA, PCPT 风险计算器,和 Huashan 风险计算器的性能到 2014 年 12 月。所有必要临床的信息在活体检视前被收集。结果证明 Huashan 冒险 1 和 2 超过了的计算器为在全部训练的队和成层的人口预言 PCa 的 PCPT 风险计算器(与到 20.0 ng m 的从 2.0 ng ml −1 的 PSA ) 。在确认学习, Huashan 风险计算器 1 仍然在全部确认队超过了 PCPT 风险计算器(0.849 对 0.779 在在操作特征的接收装置下面的区域弯[AUC ] 并且成层的人口。当 Huashan 风险计算器被使用时,不必要的活体检视(约 30%) 的可观的减小也被观察。因此,我们相信 Huashan 风险计算器(特别 Huashan 风险计算器 1 ) 可能为在中国人口预言 PCa 增加了价值。然而,这些结果仍然在更大的人口需要进一步的评估。 | Yi-Shuo Wu Ning Zhang Sheng-Hua Liu Jian-Feng Xu Shi-Jun Tong Ye-Hua Cai Li-Min Zhang Pei-De Bai Meng-Bo HU Hao-Wen Jiang Rong Na Qiang Ding Ying-Hao Sun | 2016 | Asian Journal of Andrology2016，18，6： | 7 |
| 12 | Effect of Dandelion Extracts on the Proliferation of Ovarian Granulosa Cells and Expression of Hormone Receptors显示文摘 | Tong Wang Bing Xue Hui Shao Shu-Yu Wang Li Bai Cheng-Hong Yin Huan-Ying Zhao Yong-Chao Qi Le-Le Cui Xin He Yan-Min Ma | 2018 | Chinese Medical Journal2018，，14： | 6 |
| 13 | A new method to evaluate the dose-effect relationship of a TCM formula Gegen Qinlian Decoction: "Focus" mode of integrated biomarkers显示文摘精确地评估繁体中文药(TCM ) 的功效是困难的,它导致剂量效果分析的无常和复杂性。在这研究,我们建立了 biomarkers 的焦点模式描绘 Gegen Qinlian 煎(GQD ) 的剂量效果关系,为对待类型 2 糖尿病 mellitus (2-DM ) 的一个 TCM 公式。2-DM 的一个老鼠模型通过喂的饮食与低剂量的 STZ 注射相结合的高脂肪被建立。有 2-DM 的老鼠是管理高度,中间或低剂量(6.785, 4.071, 1.357 mgl/L ) 在 vitro 引起了 glomerular podocyte apoptosis,它被无毒的 chrysin (1-20 mol/L ) dose-dependently 通过 DNA 的减小稀释破碎。Chrysin 处理 dose-dependently 恢复了增加的 Bax/Bcl-2 比率,并且在高暴露葡萄糖的肾的 podocytes 压制了 Apaf-1 正式就职和提高的细胞色素 c 版本。在糖尿病的 db/db 老鼠, chrysin 的口头的管理(10 mgf 1.84 ??? ?? | Zhi-qiang HUANG Xue-mei FAN Yi-ming WANG Qiong-lin LIANG Xiao-lin TONG Yu BAI Ying-meng LI Guo-an LUO Chao CHEN | 2017 | Acta Pharmacologica Sinica2017，38，8： | 6 |
| 14 | An antiretroviral regimen containing 6 months of stavudine followed by long-term zidovudine for first-fine HIV therapy is optimal in resource-limited settings: a prospective, multicenter study in China显示文摘 | Li Taisheng Guo Fuping Li Yijia Zhang Chengda Han Yang Lye Wei He Yun Lu Hongzhou Xie Jing Huang Aiqiong Li Yanling Tang Xiaoping Wang Hui Zhang Tong Gao Guiju Lei Junkang Zhang Xiaoying Wu Xinhua Sun Yongtao Bai Jinsong Luo Ling Wang Huanlmg | 2014 | Chinese Medical Journal2014，，1： | 6 |
| 15 | Enhanced Reconfigurable Intelligent Surface Assisted mmWave Communication: A Federated Learning显示文摘Reconfigurable intelligent surface(RIS)has been proposed as a potential solution to improve the coverage and spectrum efficiency for future wireless communication.However,the privacy of users’data is often ignored in previous works,such as the user’s location information during channel estimation.In this paper,we propose a privacy-preserving design paradigm combining federated learning(FL)with RIS in the mmWave communication system.Based on FL,the local models are trained and encrypted using the private data managed on each local device.Following this,a global model is generated by aggregating them at the central server.The optimal model is trained for establishing the mapping function between channel state information(CSI)and RIS’configuration matrix in order to maximize the achievable rate of the received signal.Simulation results demonstrate that the proposed scheme can effectively approach to the theoretical value generated by centralized machine learning(ML),while protecting user’privacy. | Lixin Li Donghui Ma Huan Ren Dawei Wang Xiao Tang Wei Liang Tong Bai | 2020 | China Communications2020，17，10： | 6 |
| 16 | [^18F]2-fluoro-2-deoxyglucose positron emission tomography/ computed tomography in predicting radiation pneumonitis显示文摘背景预防是一会儿唯一的可得到的方法限制导致放射的肺病态。一个好预言者是预防的关键点。如果,这研究试图调查[18F ] 在在放射疗法以后的肺的 2-fluoro-2-deoxyglucose (FDG ) 举起变化能为尖锐放射肺炎(RP ) 被用作一个新预言者。有肺癌症的 41 个病人经历了 FDG 正电子排放 tomography/computed 断层摄影术(FDG-PET/CT ) 的方法在放射疗法前后的成像。吝啬的标准化举起价值(SUV ) 为 0-9 Gy 的 isodose 区域被测量, 10-19 Gy, 20-29 Gy, 30-39 Gy, 40-49 Gy。在放射疗法以后的这些区域的吝啬的 SUV 与基线相比。在开发了 RP 的病人的吝啬的 SUV 在谁的那些也与那相比。统计差别被匹配的对 t 测试决定。放射治疗肿瘤学组( RTOG )标准被用于诊断并且与 12 个月的中部的后续 RP.Results 分级,(26.8%) 11 开发的 41 个病人分级 2 并且在尖锐 RP.The 上面意味着区域的 SUV ( 10-19 Gy , 20-29 Gy , 30-39 Gy , 40-49 Gy )在放射治疗分别地为 0-9 Gy , 10-19 Gy , 20-29 Gy , 30-39 Gy 和 40-49 Gy 是 0.54 , 0.68 , 1.31 , 1.74 和 2.27 以后,在所有 41 patients.The 平均数 SUV 在放射治疗以后增加了。在放射治疗前,在每个区域的吝啬的 SUV 分别地是 0.53, 0.52, 0.52, 0.53 和 0.54。这些病人在收到 10 Gy 或更多的区域举办了显著地更高的 FDG 活动( P < 0.001 )有他们的对应物的 .Compared , SUV 的举起在开发了肺织物的吝啬的 SUV 可以是的尖锐 RP subsequently.Conclusion 的那些病人是显著地更大的为尖锐 RR FDG-PET/CT 的一个有用预言者可以在肺的放射损坏的学习起一个新作用。 | SONG Hao YU Jin-ming Feng-ming Kong LU Jie BAI Tong MA Li FU Zheng | 2009 | Chinese Medical Journal2009，，11： | 5 |
| 17 | Phytohormone dynamics in developing endosperm influence rice grain shape and quality显示文摘Hormones are important signaling molecules regulating developmental processes and responses to environmental stimuli in higher plants.Rice endosperm,the portion of the seed surrounding the embryo,is the main determinant of rice grain shape and yield;how-ever,the dynamics and exact functions of phyto-hormones in developing endosperm remain elusive.Through a systemic study including transcriptome analysis,hormone measurement,and transgene-based endosperm-specific expression of phytohormone bio-synthetic enzymes,we demonstrated that dynamic phytohormone levels play crucial roles in the developing rice endosperm,particularly in regard to grain shape and quality.We detected diverse,differential,and dra-matically changing expression patterns of genes related to hormone biosynthesis and signaling during endosperm development,especially at early devel-opmental stages.Liquid chromatography measure-ments confirmed the dynamic accumulation of hor-mones in developing endosperm.Further transgenic analysis performed on plants expressing hormone bio-synthesis genes driven by an endosperm-specific pro-moter revealed differential effects of the hormones,especially auxin and brassinosteroids,in regulating grain shape and quality.Our studies help elucidate the dis-tinct roles of hormones in developing endosperm and provide novel and useful tools for influencing crop seed shape and yield. | Xiao-Fan Zhang Jian-Hua Tong Ai-Ning Bai Chun-Ming Liu Lang-Tao Xiao Hong-Wei Xue | 2020 | Journal of Integrative Plant Biology2020，62，10： | 5 |
| 18 | Prevalence of Metabolic Syndrome in Chinese Nickel-exposed Workers显示文摘The prevalence of metabolic syndrome(MS was investigated in Chinese nickel-exposed workers A total of 35 104 subjects were enrolled in this study.The age-adjusted prevalence of MS,BMI≥25diabetes,hypertension,and abnormal lipid was13.9%,29.5%,12.8%,27.5%,and 47.1%respectively.The prevalence of BMI≥25hyperglycemia,and hypertension increased | YANG Ai Min BAI Ya Na PU Hong Quan ZHENG Tong Zhang CHENG Ning LI Juan Sheng LI Hai Yan ZHANG Ya Wei DING Jiao SU Hui REN Xiao Wei HU Xiao Bin | 2014 | Biomedical and Environmental Sciences2014，27，6： | 5 |
| 19 | Polymorphisms of dihydropyrimidine dehydrogenase gene and clinical outcomes of gastric cancer patients treated with fluorouracil-based adjuvant chemotherapy in Chinese population显示文摘背景 Dihydropyrimidine 脱氢酶( DPD ),涉及5氟尿嘧啶( 5-FU )的分解代谢的关键酶,是关于这研究的 .The 目的是探索在 dihydropyrimidine 脱氢酶基因( DPYD )和在中国 population.Methods 与基于氟尿嘧啶的辅助化疗对待的胃的癌症病人的临床的结果的多型性之间的协会的 5-FU 的功效和毒性的 pharmacogenetic 研究的吸引人的候选人有气体的 362 | ZHANG Xiao-ping BAI Zhi-bin CHEN Bao-an FENG Ji-feng YAN Feng JIANG Zhi ZHONG Yue-jiao WU Jian-zhong CHEN Lu LU Zu-hong TONG Na ZHANG Zheng-dong XU-Pei-pei PENG Miao-xin ZHANG Wen-jing WANG Shuai | 2012 | Chinese Medical Journal2012，，5： | 5 |
| 20 | Aberrant methylation of SPARC in human hepatocellular carcinoma and its clinical implication显示文摘AIM:To investigate the methylation status of secreted protein acidic and rich in cysteine(SPARC) in human hepatocellular carcinoma(HCC) and evaluate its clinical implication.METHODS:The methylation status of SPARC was analyzed in one HCC cell line(SMMC-7721) and 60 pairs of HCC and corresponding nontumorous tissues by methylation-specific polymerase chain reaction and bisulfite sequencing.The expression of SPARC mRNA and protein were examined by reverse transcription polymerase chain reaction and immunohistochemistry,respectively.The correlations between the methylation status and the gene expression,the clinicopathological parameters,as well as the prognosis after surgery were analyzed.RESULTS:In the SMMC-7721 cell line,the loss of SPARC expression was correlated with the aberrant methylation and could be reactivated by the demethylating agent 5-aza-2'-deoxycytidine.Methylation frequency of SPARC in HCC was significantly higher than that in the corresponding nontumorous tissues(45/60 vs 7/60,P < 0.001),and it was correlated with the pathological classification(P = 0.019).The downregulation of the SPARC mRNA expression in HCC was correlated with the SPARC methylation(P = 0.040).The patients with methylated SPARC had a poorer overall survival than those without methylated SPARC(28.0 mo vs 41.0 mo,P = 0.043).CONCLUSION:Aberrant methylation is an important mechanism for SPARC inactivation in HCC and SPARC methylation may be a promising biomarker for the diagnosis and prognosis of HCC. | Ye Zhang Zhi Du Tong Bai Ying-Tang Gao Yi-Jun Wang Cheng Lou Feng-Mei Wang Yu Bai Bin Yang | 2012 | World Journal of Gastroenterology2012，18，17： | 4 |
您所在的机构暂未开通此部分的功能，请联系管理员