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| 1 | Correction of a genetic disease by CRISPR-Cas9-mediated gene editing in mouse spermatogonial stem cells显示文摘Spermatogonial 干细胞(SSC ) 能在移植以后生产众多的男配偶子进接受者睾丸,介绍为基因治疗和修改基因的动物的连续生产的一条珍贵途径。然而, SSC 的成功的基因操作被限制了,部分由于复杂性和当前可得到的基因编辑技术的低效率。这里,我们证明有效基因修正能用 CRISPR-Cas9 系统被介绍进 SSC。我们使用了 CRISPR-Cas9 系统变异在 SCC 的 EGFP transgene 或内长的 Crygc 基因。变异的 SSC 在移植以后经历了精子发生进不肥沃的老鼠睾丸的生精的小管。圆 spermatids 被产生并且在进成熟卵母细胞的注射以后,支持了显示相应变异的显型的异质接合的后代的生产。而且,在 Crygc 的一个引起疾病的变化(在 SSC 先存在的 Crygc −/−) 能乐意地到加入的 CRISPR-Cas9-induced nonhomologous 结束(NHEJ ) 或指导相同的修理(HDR ) 被修理,导致没有是的离开目标修正的证据带改正的基因的 SSC 线由整个染色体的定序出现。用从这些线产生的圆 spermatids 的授精在 100% 的效率与改正的显型产生了后代。我们的结果表明有效基因由 CRISPR-Cas9 系统在老鼠 SSC 编辑,并且提供在 SSC 经由基因修正治好基因疾病的原则的证明。 | Yuxuan Wu Hai Zhou Xiaoying Fan Ying Zhang Man Zhang Yinghua Wang Zhenfei Xie Meizhu Bai Qi Yin Dan Liang Wei Tang Jiaoyang Liao Chikai Zhou Wujuan Liu Ping Zhu Hongshan Guo Hong Pan Chunlian Wu Huijuan Shi Ligang Wu Fuchou Tang Jinsong Li | 2015 | Cell Research2015，25，1： | 37 |
| 2 | Influence of catgut implantation at acupoints on leptin and insulin resistance in simple obesity rats显示文摘OBJECTIVES:To investigate the influence of catgut implantation at acupoints on leptin resistance(LR) and insulin resistance(IR) in the simple obesity rat.METHODS:Rats were made obese with high-fat diets,after which surgical catgut was implanted at Zusanli(ST 36) and Neiting(ST 44) acupoints once a week for 4 weeks(implantation group).Rats from the implantation group were compared with normal rats and unoperated obese rats(control group).Gene expression of the leptin receptor(OB-R) was evaluated using RT-PCR and northern blot.Serum and hypothalamus leptin and insulin(INS) levels were determined by radioimmunoassay.Body weight,Lee's index,body fat,serum and hypothalamus leptin and INS levels,and hypothalamic OB-R gene expression were determined before and after treatment.RESULTS:Body weights,Lee's index,body fat,and serum leptin and INS levels were significantly higher in obese than in normal rats.Hypothalamic leptin and INS levels and OB-R gene expression were significantly lower in obese rats.Catgut implantation at acupoint promoted weight loss and decreased serum leptin and INS levels.Hypothalamic leptin and INS levels and OB-R gene expression increased significantly.CONCLUSIONS:Catgut implantation at acupoint adjusts central and peripheral leptin and promotes hypothalamic OB-R gene expression.This may be an important method for regulation of LR,IR and abnormal endocrinology and metabolism. | Runhu Yan Xiaoming Liu Jie Bai Jiang Yu Jinsong Gu | 2012 | Journal of Traditional Chinese Medicine2012，32，3： | 12 |
| 3 | Antimony oxidation and adsorption by in-situ formed biogenic Mn oxide and Fe–Mn oxides显示文摘Antimony(Sb), which can be toxic at relatively low concentrations, may co-exist with Mn(Ⅱ)and/or Fe(Ⅱ) in some groundwater and surface water bodies. Here we investigated the potential oxidation and adsorption pathways of Sb(Ⅲ and V) species in the presence of Mn(Ⅱ) and Mn-oxidizing bacteria, with or without Fe(Ⅱ). Batch experiments were conducted to determine the oxidation and adsorption characteristics of Sb species in the presence of biogenic Mn oxides(BMOs), which were formed in-situ via the oxidation of Mn(Ⅱ) by a Mn-oxidizing bacterium(Pseudomonas sp. QJX-1). Results indicated that Sb(Ⅲ) ions could be oxidized to Sb(V) ions by BMO, but only Sb(V) originating from Sb(Ⅲ) oxidation was adsorbed effectively by BMO. Introduced Fe(Ⅱ) was chemically oxidized to Fe OOH, the precipitates of which mixed with BMO to form a new compound, biogenic Fe–Mn oxides(BFMO). The BMO part of the BFMO mainly oxidized and the Fe OOH of the BFMO mainly adsorbed the Sb species. In aquatic solutions containing both As(Ⅲ) and Sb(Ⅲ), the BFMO that formed in-situ preferentially oxidized Sb over As but adsorbed As more efficiently. Chemical analysis and reverse transcription real-time polymerase chain reaction revealed that the presence of Fe(Ⅱ), As(Ⅲ) and Sb(Ⅲ) accelerated the oxidation of Mn(Ⅱ) but inhibited the activity of Mn-oxidizing bacteria. These results provide significant insights into the biogeochemical pathways of Sb, Mn(Ⅱ) in aquatic ecosystems, with or without Fe(Ⅱ). | Yaohui Bai William A.Jefferson Jinsong Liang Tingting Yang Jiuhui Qu | 2017 | Journal of Environmental Sciences2017，29，4： | 12 |
| 4 | An antiretroviral regimen containing 6 months of stavudine followed by long-term zidovudine for first-fine HIV therapy is optimal in resource-limited settings: a prospective, multicenter study in China显示文摘 | Li Taisheng Guo Fuping Li Yijia Zhang Chengda Han Yang Lye Wei He Yun Lu Hongzhou Xie Jing Huang Aiqiong Li Yanling Tang Xiaoping Wang Hui Zhang Tong Gao Guiju Lei Junkang Zhang Xiaoying Wu Xinhua Sun Yongtao Bai Jinsong Luo Ling Wang Huanlmg | 2014 | Chinese Medical Journal2014，，1： | 6 |
| 5 | The RNA-binding protein ROD1/PTBP3 cotranscriptionally defines AID-loading sites to mediate antibody class switch in mammalian genomes显示文摘 | Juan Chen Zhaokui Cai Meizhu Bai Xiaohua Yu Chao Zhang Changchang Cao Xihao Hu Lei Wang Ruibao Su Di Wang Yingpeng Yao Rong Ye Baidong Hou Yang Yu Shuyang Yu Jinsong Li Yuanchao xue | 2018 | Cell Research2018，28，10： | 4 |
| 6 | Design of 4D printed shape-changing tracheal stent and remote controlling actuation显示文摘has a good application prospect.The biodegradable stent can effectively reduce the damage to patients and improve the therapeutic performance of stents.In this work,a series of shape memory polylactic acid(Fe_(3)O_(4))composite tracheal stents were manufactured by 4D printing.The composite tracheal stents with different structures were designed.Moreover,with the addition of magnetic particles Fe3 O4,the shape memory PLA/Fe_(3)O_(4)composite tracheal stent has a magnetic driving effect.Under the magnetic field,the shape recovery process is completed within 40 s,and the shape recovery rate is more than 99%.Moreover,the 4D printed tracheal stent was also triggered by the irradiation of infrared lamp to realize the remote controlling recovery.The research on the structure design and driving method of 4D printing tracheal stent expands the application scope of shape memory polymer composites in biomedical field,provides a new way for personalized implantable medical devices and minimally invasive surgery.It is of great significance for better precision medical treatment. | Fenghua Zhang Nan Wen Linlin Wang Yunqi Bai Jinsong Leng | 2021 | International Journal of Smart and Nano Materials2021，12，4： | 3 |
| 7 | Relationships between interspecific differences in the mass of internal organs,biochemical markers of metabolic activity,and the thermogenic properties of three smallpasserines显示文摘Background:The capacity for thermogenesis is considered part of an animal's adaptive strategy for survival,and basal metabolic rate(BMR) is one of the fundamental physiological standards for assessing the energy cost of thermoregulation in endotherms.BMR has been shown to be a highly flexible phenotypic trait both between,and within,species,but the metabolic mechanisms involved in the regulation of BMR,which range from variation in organ mass to biochemical adjustments,remain unclear.In this study,we investigated the relationship between organ mass,biochemical markers of metabolic tissue activity,and thermogenesis,in three species of small passerines:wild Bramblings(Fringilla montifringilla),Little Buntings(Emberiza pusilla) and Eurasian Tree Sparrows(Passer montanus),caught in Wenzhou,southeastern China.Methods:Oxygen consumption was measured using an open-circuit respirometry system.Mitochondrial state-4 respiration and cytochrome c oxidase(COX) activity in liver and pectoral muscle were measured with a Clark electrode.Results:Our results show that Eurasian Tree Sparrows had significantly higher BMR,digestive organ mass,mitochondrial state-4 respiration capacity and COX activity in liver and muscle,than Bramblings and Little Buntings.Furthermore,interspecific differences in BMR were strongly correlated with those indigestive tract mass,state-4 respiration and COX activity.Conclusions:Our findings suggest that the digestive organ mass,state-4 respiration and COX activity play an important role in determining interspecific differences in BMR. | Minlan Bai Xujian Wu Kejing Cai Weihong Zheng Jinsong Liu | 2016 | Chinese Birds2016，，2： | 2 |
| 8 | Correction of a Genetic Disease in Mouse via Use of CRISPR-Cas9显示文摘 | Yuxuan Wu Dan Liang Yinghua Wang Meizhu Bai Wei Tang Shiming Bao Zhiqiang Yan Dangsheng Li Jinsong Li | 2013 | Cell Stem Cell2013，，6： | 2 |
| 9 | Characterizations of Mg-W System Graded-Density Impactors for Complex Loading Experiments显示文摘 | Guoqiang Luo Jinsong Bai Hua Tan Qiang Shen Jianbo Hu Qingsong Wang Chengda Dai Qiang Wu Lianmeng Zhang | 2010 | Metallurgical and Materials Transactions A2010，，9： | 1 |
| 10 | The Solar Upper Transition Region Imager(SUTRI)Onboard the SATech-01 Satellite显示文摘The Solar Upper Transition Region Imager(SUTRI)onboard the Space Advanced Technology demonstration satellite(SATech-01),which was launched to a Sun-synchronous orbit at a height of~500 km in 2022 July,aims to test the on-orbit performance of our newly developed Sc/Si multi-layer reflecting mirror and the 2k×2k EUV CMOS imaging camera and to take full-disk solar images at the Ne VII 46.5 nm spectral line with a filter width of~3 nm.SUTRI employs a Ritchey-Chrétien optical system with an aperture of 18 cm.The on-orbit observations show that SUTRI images have a field of view of~416×416 and a moderate spatial resolution of~8″without an image stabilization system.The normal cadence of SUTRI images is 30 s and the solar observation time is about16 hr each day because the earth eclipse time accounts for about 1/3 of SATech-01's orbit period.Approximately15 GB data is acquired each day and made available online after processing.SUTRI images are valuable as the Ne VII 46.5 nm line is formed at a temperature regime of~0.5 MK in the solar atmosphere,which has rarely been sampled by existing solar imagers.SUTRI observations will establish connections between structures in the lower solar atmosphere and corona,and advance our understanding of various types of solar activity such as flares,filament eruptions,coronal jets and coronal mass ejections. | Xianyong Bai Hui Tian Yuanyong Deng Zhanshan Wang Jianfeng Yang Xiaofeng Zhang Yonghe Zhang Runze Qi Nange Wang Yang Gao Jun Yu Chunling He Zhengxiang Shen Lun Shen Song Guo Zhenyong Hou Kaifan Ji Xingzi Bi Wei Duan Xiao Yang Jiaben Lin Ziyao Hu Qian Song Zihao Yang Yajie Chen Weidong Qiao Wei Ge Fu Li Lei Jin Jiawei He Xiaobo Chen Xiaocheng Zhu Junwang He Qi Shi Liu Liu Jinsong Li Dongxiao Xu Rui Liu Taijie Li Zhenggong Feng Yamin Wang Chengcheng Fan Shuo Liu Sifan Guo Zheng Sun Yuchuan Wu Haiyu Li Qi Yang Yuyang Ye Weichen Gu Jiali Wu Zhe Zhang Yue Yu Zeyi Ye Pengfeng Sheng Yifan Wang Wenbin Li Qiushi Huang Zhong Zhang | 2023 | Research in Astronomy and Astrophysics2023，23，6： | 1 |
| 11 | Spermatogenic Cell-Specific Gene Mutation in Mice via CRISPR-Cas9显示文摘Tissue-specific knockout technology enables the analysis of the gene function in specific tissues in adult mammals.However,conventional strategy for producing tissue-specific knockout mice is a time- and labor-consuming process,restricting rapid study of the gene function in vivo.CRISPR-Cas9 system from bacteria is a simple and efficient gene-editing technique,which has enabled rapid generation of gene knockout lines in mouse by direct injection of CRISPR-Cas9 into zygotes.Here,we demonstrate CRISPR-Cas9-mediated spermatogenic cell-specific disruption of Scp3 gene in testes in one step.We first generated transgenic mice by pronuclear injection of a plasmid containing Hspa2 promoter driving Cas9 expression and showed Cas9 specific expression in spermatogenic cells.We then produced transgenic mice carrying Hspa2 promoter driven Cas9 and constitutive expressed sgRNA targeting Scp3 gene.Male founders were infertile due to developmental arrest of spermatogenic cells while female founders could produce progeny normally.Consistently,male progeny from female founders were infertile and females could transmit the transgenes to the next generation.Our study establishes a CRISPR-Cas9-based one-step strategy to analyze the gene function in adult tissues by a temporal-spatial pattern. | Meizhu Bai Dan Liang Yinghua Wang Qing Li Yuxuan Wu Jinsong Li | 2016 | Journal of Genetics and Genomics2016，43，5： | 1 |
| 12 | Characterizations of Mg-W System Graded-Density Impactors for Complex Loading Experiments显示文摘 | Guoqiang Luo Jinsong Bai Hua Tan Qiang Shen Jianbo Hu Qingsong Wang Chengda Dai Qiang Wu Lianmeng Zhang | 2010 | Metallurgical and Materials Transactions A2010，，9： | 1 |
| 13 | Direct infection of SARS-CoV-2 in human iPSC-derived 3D cardiac organoids recapitulates COVID-19 myocarditis显示文摘Dear editor,The pandemic of coronavirus disease-19(COVID-19)has lasted for more than three years,causing lots of extra death and posing a serious threat to public health.This pandemic swept the last global safety island in a matter of months,coinciding with the switch of zero-COVID policy of China.The causative agent,SARS-CoV-2,spreads globally and then lurks in the population with sporadic outbreaks in local areas.Meanwhile,the sequelae of convalescent COVID-19 patients receded slowly.The pantissue tropism of SARS-CoV-2 results in a wide range of symptoms in patients,including cardiovascular disorders.Cardiac complications occur in 20%–44%of hospitalized patients,which is an independent risk factor for COVID-19 mortality(Ma et al.,2022;Patone et al.,2022).Survivors of acute COVID-19 also have a substantial risk of cardiovascular disease burden lasting up to one year(Xie et al.,2022). | Weijie Wang Jinxuan Yang Pinfang Kang Jinsong Bai Xiaoli Feng Liuqi Huang Yiming Zhang Yuechun Wu Bi Tang Hongju Wang JianJie Jiang Minghua Li Bing Zhao Xinglou Yang | 2023 | Virologica Sinica2023，38，6： | 0 |
| 14 | Quantized anomalous Hall resistivity achieved in molecular beam epitaxy-grown MnBi_(2)Te_(4)thin films显示文摘The intrinsic magnetic topological insulator MnBi_(2)Te_(4)provides a feasible pathway to the high-temperature quantum anomalous Hall(QAH)effect as well as various novel topological quantum phases.Although quantized transport properties have been observed in exfoliated MnBi_(2)Te_(4)thin flakes,it remains a big challenge to achieve molecular beam epitaxy(MBE)-grown MnBi_(2)Te_(4)thin films even close to the quantized regime.In this work,we report the realization of quantized anomalous Hall resistivity in MBE-grown MnBi_(2)Te_(4)thin films with the chemical potential tuned by both controlled in situ oxygen exposure and top gating.We find that elongated post-annealing obviously elevates the temperature to achieve quantization of the Hall resistivity,but also increases the residual longitudinal resistivity,indicating a picture of high-quality QAH puddles weakly coupled by tunnel barriers.These results help to clarify the puzzles in previous experimental studies on MnBi_(2)Te_(4)and to find a way out of the big difficulty in obtaining MnBi_(2)Te_(4)samples showing quantized transport properties. | Yunhe Bai Yuanzhao Li Jianli Luan Ruixuan Liu Wenyu Song Yang Chen Peng-Fei Ji Qinghua Zhang Fanqi Meng Bingbing Tong Lin Li Yuying Jiang Zongwei Gao Lin Gu Jinsong Zhang Yayu Wang Qi-Kun Xue Ke He Yang Feng Xiao Feng | 2024 | National Science Review2024，11，2： | 0 |
| 15 | A dynamic-inner LSTM prediction method for key alarm variables forecasting in chemical process显示文摘With the increase in the complexity of industrial system, simply detecting and diagnosing a fault may be insufficient in some cases, and prognosing the fault ahead of time could have a certain necessity. Accurate prediction of key alarm variables in chemical process can indicate the possible change to reduce the probability of abnormal conditions. According to the characteristics of chemical process data, this work proposed a key alarm variables prediction model in chemical process based on dynamic-inner principal component analysis(DiPCA) and long short-term memory(LSTM). DiPCA is used to extract the most dynamic components for prediction. While LSTM is used to learn the relationship and predict the key alarm variables. This work used a simulation data set and a real hydrogenation process data set for applications and explained the model validity from the essential characteristics. Comparison of results with different models shows that our model has better prediction accuracy and performance, which can provide the basis for fault prognosis and health management. | Yiming Bai Shuaiyu Xiang Feifan Cheng Jinsong Zhao | 2023 | Chinese Journal of Chemical Engineering2023，55，3： | 0 |
| 16 | Space advanced technology demonstration satellite显示文摘The Space Advanced Technology demonstration satellite(SATech-01),a mission for low-cost space science and new technology experiments,organized by Chinese Academy of Sciences(CAS),was successfully launched into a Sun-synchronous orbit at an altitude of~500 km on July 27,2022,from the Jiuquan Satellite Launch Centre.Serving as an experimental platform for space science exploration and the demonstration of advanced common technologies in orbit,SATech-01 is equipped with 16 experimental payloads,including the solar upper transition region imager(SUTRI),the lobster eye imager for astronomy(LEIA),the high energy burst searcher(HEBS),and a High Precision Magnetic Field Measurement System based on a CPT Magnetometer(CPT).It also incorporates an imager with freeform optics,an integrated thermal imaging sensor,and a multi-functional integrated imager,etc.This paper provides an overview of SATech-01,including a technical description of the satellite and its scientific payloads,along with their on-orbit performance. | ZHANG XiaoFeng CHEN Wen ZHU XiaoCheng MENG Na HE JunWang BI XingZi ZHANG YongHe SHI Qi LI Fei LIU Rui FENG ZhengGong LIU Liu LI JinSong WU HaiChen XU DongXiao LI TaiJie HUANG JiangJiang LIU Shuo LI TianTong YU XianSheng GAO Yang ZHOU Heng BAN HanYu ZHANG YanLi ZHANG YueTing YANG YingQuan HE Tao DUAN XuLiang CHEN Xin WANG YaMin SUN AnTai ZHANG KuoXiang SUN Ying WANG YaoBin FAN ChengCheng XIONG ShaoLin LI XinQiao WEN XiangYang LING ZhiXing SUN XiaoJin ZHANG Chen BAI XianYong WANG ZhanShan DENG YuanYong TIAN Hui YANG JianFeng XUE HongBo SANG Peng LIU JinGuo ZHENG HuiLong ZHU Xiang HE JianWu LI Hui XU LuXiang XU ShuYan CHEN WenWu LIU ZhenDong WANG ZhaoLi MAO XiangLong GAO Rong LI ZongXuan DING GuoPeng WANG XinYu DOU RunJiang WENG LuBin LUO Hao WANG YaPing LIANG XianFeng FANG ZiRuo | 2024 | Science China(Technological Sciences)2024，67，1： | 0 |
| 17 | Epilithic biofilm as a reservoir for functional virulence factors in wastewater-dominant rivers after WWTP upgrade显示文摘Virulence factors(VFs)confer upon pathogens the ability to cause various types of damage or diseases.Wastewater treatment plants(WWTPs)are important point sources for the emission of pathogens and VFs into receiving rivers.Conventional WWTP upgrades are often implemented to improve the water quality of receiving ecosystems.However,knowledge on the pathogens,VFs,and health risks to receiving aquatic ecosystems after upgrade remains limited.In this study,we investigated detailed pathogenic information,including taxa,pathogenicity,and health risk,in two wastewater-dominant rivers after WWTP upgrade.Using 16S rRNA gene sequencing,we screened 14 potential pathogens in water and epilithic biofilm samples,though they were significantly more enriched in the biofilms.Combining 16S rRNA and metagenomic sequencing data,we identified Pseudomonas and Aeromonas as the dominant pathogenic taxa carrying functional VFs(e.g.,mobility and offensive)in the epilithic biofilm.Moreover,strong pathogen-specific VF-host co-occurrence events were observed in the epilithic biofilm samples,indicating the importance of biofilms as reservoirs and vehicles for VFs.Further,we demonstrated that mobility VF is crucial for biofilm formation and pathogens in biofilm carrying offensive VF may be highly invasive.Quantification and health risk assessment suggested that the skin contact risk of P.aeruginosa carrying VFs was higher than the acceptable probability of 10^(-4)in both water and epilithic biofilm samples,which may threaten ecological and human health. | Guannan Mao Jinsong Liang Qiaojuan Wang Chen Zhao Yaohui Bai Ruiping Liu Huijuan Liu Jiuhui Qu | 2021 | Journal of Environmental Sciences2021，33，3： | 0 |
| 18 | Gonadal mosaicism mediated female-biased gender control in mice显示文摘Dear Editor, In nature or during artificial breeding,evolutionary interven-tion or desirable trait selection could be achieved by means of gender control in an organism.Previously reported approaches including sex-sorted semen and genetic manipulation of a certain gene(Holden and Butler,2018;Yosef et al.,2019).In mice,a recent study demonstrated a genetic system for biasing sex ratio through crossing two genetically engineered mouse lines(Yosef et al.,2019).The maternal line encodes functional Cas9 protein on an auto-somal chromosome,whereas the paternal encodes guide RNAs on Y chromosome targeting vital genes.After fertil-ization,males carrying both Cas9 and guide RNAs were self-destructed,resulting in a female biased sex ratio.However,the litter size is proximate half of normal size since half of the progeny are eliminated.Moreover,a reproductive reservoir of males and females must be maintained for crossing.Here,we established a mouse line carrying Hspa2 promoted Cas9 and constitutively expressed sgRNA targeting repetitive sequences on Y chromosome.In those mice,spermatogenic cells carrying Y chromosome were eliminated during sper-matogenesis,resulting in female biased sex ratio in proge-nies whereas the total number was unaffected. | Meizhu Bai Dan Liang Yan Cheng Guolong Liu Qiudao Wang Jinsong Li Yuxuan Wu | 2022 | Protein & Cell2022，13，11： | 0 |
| 19 | L-Fucose inhibits the progression of cholangiocarcinoma by causing microRNA-200b overexpression显示文摘Background:Cholangiocarcinoma(CCA)is a malignant biliary tract tumor with an extremely poor prognosis.There is an urgent demand to explore novel therapeutic strategies.L-fucose has been confirmed to participate in anti-inflammation and antitumor activities.However,the effect of L-fucose on the progression of CCA has not been well investigated.This study aimed to determine whether L-fucose induced the inhibition of CCA and its possible mechanism.Methods:The anti-growth activity was determined using Cell Counting Kit-8 assay,colony formation assays,Annexin V-fluorescein isothiocyanate/propidium iodide(FITC/PI)assay,and cell cycle analysis.The anti-metastasis activity was determined by wound healing,transwell,and invasion assays.The anti-angiogenesis activity was determined by tube formation and transwell assays.MicroRNAs that may be involved in the L-fucose-induced CCA inhibition was analyzed using bioinformatics methods.The preclinical therapeutic efficacy was mainly estimated by ultrasound in xenograft nude mouse models.Differences were analyzed via Student’s t test or one-way analysis of variance.Results:L-Fucose induced apoptosis and G0/G1 cell cycle arrest,inhibited cell epithelial-mesenchymal transition of CCA cells,and additionally inhibited tube formation of human umbilical vein endothelial cells(HUVECs)in a dose-dependent manner,leading to a decrease in cell proliferation,metastasis,and angiogenesis.Mechanistically,L-fucose induced microRNA-200b(miR-200b)upregulation,and mitogen-activated protein kinase 7(MAPK7)downregulation was found to be targeted by miR-200b,with decreased cell proliferation and metastasis.Additionally,phosphorylated signal transducer and activator of transcription 3 was found to be downregulated after L-fucose treatment.Finally,in vivo experiments in CCA xenograft models also confirmed the antitumor properties of L-fucose.Conclusion:L-Fucose inhibited the progression of CCA via the miR-200b/MAPK7 and signal transducer and activator of transcription 3 signaling pathways. | Biqiang Zhu Jingjing Zheng Gaichao Hong Tao Bai Wei Qian Jinsong Liu Xiaohua Hou | 2022 | Chinese Medical Journal2022，135，24： | 0 |
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