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| 1 | Inhibition of SARS-CoV-2 (previously 2019-nCoV) infection by a highly potent pan-coronavirus fusion inhibitor targeting its spike protein that harbors a high capacity to mediate membrane fusion显示文摘The recent outbreak of coronavirus disease(COVID-19)caused by SARS-CoV-2 infection in Wuhan,China has posed a serious threat to global public health.To develop specific anti-coronavirus therapeutics and prophylactics,the molecular mechanism that underlies viral infection must first be defined.Therefore,we herein established a SARS-CoV-2 spike(S)protein-mediated cell-cell fusion assay and found that SARS-CoV-2 showed a superior plasma membrane fusion capacity compared to that of SARS-CoV.We solved the X-ray crystal structure of six-helical bundle(6-HB)core of the HR1 and HR2 domains in the SARS-CoV-2 S protein S2 subunit revealing that several mutated amino acid residues in the HR1 domain may be associated with enhanced interactions with the HR2 domain.We previously developed a pan-coronavirus fusion inhibitor,EK1,which targeted the HR!domain and could inhibit infection by divergent human coronaviruses tested,including SARS-CoV and MERS-CoV.Here we generated a series of lipopeptides derived from EK1 and found that EK1C4 was the most potent fusion inhibitor against SARS-CoV-2 S protein-mediated membrane fusion and pseudovirus infection with IC50s of 1.3 and 15.8 nM,about 241-and 149-fold more potent than the original EK1 peptide,respectively.EK1C4 was also highly effective against membrane fusion and infection of other human coronavirus pseudoviruses tested,including SARS-CoV and MERS-CoV,as well as SARSr-CoVs,and potently inhibited the replication of 5 live human coronaviruses examined,including SARS-CoV-2.Intranasal application of EK1C4 before or after challenge with HCoV-OC43 protected mice from infection,suggesting that EK1C4 could be used for prevention and treatment of infection by the currently circulating SARS-CoV-2 and other emerging SARSr-CoVs. | Shuai Xia Meiqin Liu Chao Wang Wei Xu Qiaoshuai Lan Siliang Feng Feifei Qi Linlin Bao Lanying Du Shuwen Liu Chuan Qin Fei Sun Zhengli Shi Yun Zhu Shibo Jiang Lu Lu | 2020 | Cell Research2020，30，4： | 81 |
| 2 | A novel strong tracking cubature Kalman filter and its application in maneuvering target tracking显示文摘The fading factor exerts a significant role in the strong tracking idea. However, traditional fading factor introduction method hinders the accuracy and robustness advantages of current strong-tracking-based nonlinear filtering algorithms such as Cubature Kalman Filter(CKF) since traditional fading factor introduction method only considers the first-order Taylor expansion. To this end, a new fading factor idea is suggested and introduced into the strong tracking CKF method.The new fading factor introduction method expanded the number of fading factors from one to two with reselected introduction positions. The relationship between the two fading factors as well as the general calculation method can be derived based on Taylor expansion. Obvious superiority of the newly suggested fading factor introduction method is demonstrated according to different nonlinearity of the measurement function. Equivalent calculation method can also be established while applied to CKF. Theoretical analysis shows that the strong tracking CKF can extract the thirdorder term information from the residual and thus realize second-order accuracy. After optimizing the strong tracking algorithm process, a Fast Strong Tracking CKF(FSTCKF) is finally established. Two simulation examples show that the novel FSTCKF improves the robustness of traditional CKF while minimizing the algorithm time complexity under various conditions. | An ZHANG Shuida BAO Fei GAO Wenhao BI | 2019 | Chinese Journal of Aeronautics2019，32，11： | 22 |
| 3 | Age-related rhesus macaque models of COVID-19显示文摘Background:Since December 2019,an outbreak of the Corona Virus disease 2019(COVID-19)caused by severe acute respiratory syndrome coronavirus(SARS-CoV-2)in Wuhan,China,has become a public health emergency of international concern.The high fatality of aged cases caused by SARS-CoV-2 was a need to explore the possible age-related phenomena with non-human primate models.Methods:Three 3-5 years old and two 15 years old rhesus macaques were intratracheally infected with SARS-CoV-2,and then analyzed by clinical signs,viral replication,chest X-ray,histopathological changes and immune response.Results:Viral replication of nasopharyngeal swabs,anal swabs and lung in old monkeys was more active than that in young monkeys for 14 days after SARS-CoV-2 challenge.Monkeys developed typical interstitial pneumonia characterized by thickened alveolar septum accompanied with inflammation and edema,notably,old monkeys exhibited diffuse severe interstitial pneumonia.Viral antigens were detected mainly in alveolar epithelial cells and macrophages.Conclusion:SARS-CoV-2 caused more severe interstitial pneumonia in old monkeys than that in young monkeys.Rhesus macaque models infected with SARS-CoV-2 provided insight into the pathogenic mechanism and facilitated the development of vaccines and therapeutics against SARS-CoV-2 infection. | Pin Yu Feifei Qi Yanfeng Xu Fengdi Li Peipei Liu Jiayi Liu Linlin Bao Wei Deng Hong Gao Zhiguang Xiang Chong Xiao Qi Lv Shuran Gong Jiangning Liu Zhiqi Song Yajin Qu Jing Xue Qiang Wei Mingya Liu Guanpeng Wang Shunyi Wang Haisheng Yu Xing Liu Baoying Huang Wenling Wang Li Zhao Huijuan Wang Fei Ye Weimin Zhou Wei Zhen Jun Han Guizhen Wu Qi Jin Jianwei Wang Wenjie Tan Chuan Qin | 2020 | Animal Models and Experimental Medicine2020，3，1： | 22 |
| 4 | Analysis of characteristics and mechanism of current system on the west coast of Guangdong of China in summer显示文摘On the basis of data of drifting bottles’ tracks and the current measured in anchored stations, as well as temperature and salinityobserved in cruise investigations and coastal stations, ADCP current data and AVHRR surface sea temperature (SST) data on thewestern coast of Guangdong, synthetic results of analysis showed that the coastal currents in the west of the mouth of the ZhujiangRiver were mainly westward in summer, which constituted the north branch of cyclonic gyre in the east of the Qiongzhou Straits. Partof its water flowed westward into the Beibu Gulf through the Qiongzhou Straits. The coastal current pattern was not identical with thetraditional current system which flowed westward in the Qiongzhou Straits in winter and eastward in summer. The summertime’ scoastal current was always westward, maybe temporarily turning northeast only when the southwest wind was strong. The importantcharacteristics of coastal current on the western coast of Guangdong, in the Qiongzhou Straits and in the north of the Beibu Gulf wereanalyzed and their mechanisms also were explained. | BAO Xianwen HOU Yijun CHEN Changshen CHEN Fei SHI Maochong | 2005 | Acta Oceanologica Sinica2005，24，4： | 16 |
| 5 | Efficient generation of transgenic chickens using the spermatogonial stem cells in vivo and ex vivo transfection显示文摘The highly efficient novel methods to produce transgenic chickens were established by directly in-jecting the recombinant plasmid containing green fluorescent protein (GFP) gene into the cock's testis termed as testis-medianted gene transfer (TMGT), and transplanting transfected spermatogonial stem cells (TTSSCs). For the TMGT approach,four dosages of pEGFP-N1 DNA/cationic polymer complex were injected intratesticularly. The results showed: (1) 48 h after the injection,the percentages of testis cells expressing GFP were 4.0%, 8.7%, 10.2% and 13.6% in the 50, 100, 150 and 200 μg/mL group, re-spectively. The difference from the four dosage groups was significant (P<0.05). On day 25 after the injection, a dosage-dependent and time-dependent increase in the number of transgenic sperm was observed. The percentages of gene expression reached the summit and became stable from day 70 to 160, being 12.7%, 12.8%, 15.9% and 19.1%, respectively. The difference from the four dosage groups was also significant (P<0.05). (2) 70 d after the injection, strong green fluorescent could be observed in the seminiferous tubules by whole-mount in-situ hybridization. (3) 70 d after the injection, the semen was collected and used to artificially inseminate wild-type females. The blastoderms of F1 and F2 transgenic chicken expressed GFP were 56.2% (254/452) and 53.2% (275/517), respectively. The detec-tion of polymerase chain reaction (PCR) of F1 and F2 transgenic chicken blood genomic DNA showed that 56.5% (3/23) of F1 and 52.9% (9/17) of F2 were positive. Southern blot showed GFP DNA was in-serted in their genomic DNAs. (4) Frozen whole mount tissue sections of F1 and F2 transgenic chicken liver, heart, kidney and muscle showed that the rates of green fluorescent positive were between 50.0% and 66.7%. (5) With the TTSSCs method, SSCs ex vivo transfected with GFP were transplanted into recipient roosters whose endogenic SSCs had been resoluted. The donor SSCs settled and GFP ex-pression became readily detectable in the frozen whole mount tissue sections of recepient testes. Moreover, sperms carrying GFP could be produced normally. The results of artificially inseminating wild-type females with these sperms showed 12.5% (8/64) of offspring embryo expressed GFP and 11.1% (2/18) hatched chicks were tested transgenic. Our data therefore suggest TMGT and TTSSCs are the feasible methods for the generation of transgenic chickens. | LI BiChun SUN GuoBo SUN HuaiChang XU Qi GAO Bo ZHOU GuanYue ZHAO WenMing WU XinSheng BAO WenBin YU Fei WANG KeHua CHEN GuoHong | 2008 | Science China(Life Sciences)2008，51，8： | 13 |
| 6 | SOCS1/JAK2/STAT3 axis regulates early brain injury induced by subarachnoid hemorrhage via inflammatory responses显示文摘The SOCS1/JAK2/STAT3 axis is strongly associated with tumor growth and progression,and participates in cytokine secretion in many diseases.However,the effects of the SOCS1/JAK2/STAT3 axis in experimental subarachnoid hemorrhage remain to be studied.A subarachnoid hemorrhage model was established in rats by infusing autologous blood into the optic chiasm pool.Some rats were first treated with JAK2/STAT3 small interfering RNA(Si-JAK2/Si-STAT3)or overexpression plasmids of JAK2/STAT3.In the brains of subarachnoid hemorrhage model rats,the expression levels of both JAK2 and STAT3 were upregulated and the expression of SOCS1 was downregulated,reaching a peak at 48 hours after injury.Simultaneously,the interactions between JAK2 and SOCS1 were reduced.In contrast,the interactions between JAK2 and STAT3 were markedly enhanced.Si-JAK2 and Si-STAT3 treatment alleviated cortical neuronal cell apoptosis and necrosis,destruction of the blood-brain barrier,brain edema,and cognitive functional impairment after subarachnoid hemorrhage.This was accompanied by decreased phosphorylation of JAK2 and STAT3 protein,decreased total levels of JAK2 and STAT3 protein,and increased SOCS1 protein expression.However,overexpression of JAK2 and STAT3 exerted opposite effects,aggravating subarachnoid hemorrhage-induced early brain injury.Si-JAK2 and Si-STAT3 inhibited M1-type microglial conversion and the release of pro-inflammatory factors(inducible nitric oxide synthase,interleukin-1β,and tumor necrosis factor-α)and increased the release of anti-inflammatory factors(arginase-1,interleukin-10,and interleukin-4).Furthermore,primary neurons stimulated with oxyhemoglobin were used to simulate subarachnoid hemorrhage in vitro,and the JAK2 inhibitor AG490 was used as an intervention.The in vitro results also suggested that neuronal protection is mediated by the inhibition of JAK2 and STAT3 expression.Together,our findings indicate that the SOCS1/JAK2/STAT3 axis contributes to early brain injury after subarachnoid hemorrhage both in vitro and in vivo by inducing inflammatory responses.This study was approved by the Animal Ethics Committee of Anhui Medical University and the First Affiliated Hospital of University of Science and Technology of China(approval No.LLSC-20180202)on March 1,2018. | Yang Wang Xiang-Qian Kong Fei Wu Bin Xu De-Jun Bao Chuan-Dong Cheng Xiang-Ping Wei Yong-Fei Dong Chao-Shi Niu | 2021 | Neural Regeneration Research2021，16，12： | 11 |
| 7 | Effect of steam-flaking on chemical compositions,starch gelatinization,in vitro fermentability,and energetic values of maize,wheat and rice显示文摘Intact and steam-flaked grains of maize,wheat and rice(with whole hulls) were analyzed for chemical composition,starch gelatinization degree(SGD) and in vitro fermentation characteristics to investigate the influence of cereal type and steam-flaking(SF) processing on their nutritive values.The treatments were arranged in a 3×2 factorial design.Obvious differences(P<0.001) in chemical composition and energetic values were observed among the different cereal types.SGD and gas production(GP) rate was significantly increased(P<0.001) by SF processing.SF processing also increased(P<0.01) the proportion of propionic acid and decreased the acetic:propionic acid ratio in vitro.Steam-flaking also increased organic matter digestibility and the energetic value of the cereal grains,especially rice.Based on these results,rice probably is more amendable to SF processing than maize and wheat.In conclusion,it is feasible to partially substitute maize grain with wheat or rice in ruminant diets,and steam-flaking can significantly improve the nutritional value of wheat and rice grains. | QIAO Fu-qiang WANG Fei REN Li-ping ZHOU Zhen-ming MENG Qing-xiang BAO Yu-hong | 2015 | Journal of Integrative Agriculture2015，14，5： | 11 |
| 8 | Features of colorectal cancer in China stratified by anatomic sites:A hospital-based study conducted in university-affiliated hospitals from 2014 to 2018显示文摘Objective:The clinical and biological characteristics of colorectal cancer have been found to differ depending on the anatomic site of the cancer.However,for Chinese patients,there is limited information on the proportion of cases at each site and the related features.In this study,we explored the location,distribution and other features of colorectal cancers at each anatomic site in Chinese patients.Methods:We conducted a hospital-based study using hospitalization summary reports from 10 Peking University-affiliated hospitals from 2014 to 2018;the reports covered a total of 2,097,347 hospitalizations.Incident cases were chosen as the study population,and their epidemiological features were further analyzed.Results:A total of 20,739 colorectal cancer patients were identified.Rectum was the most common location(48.3%)of the cancer,whereas the proportions of patients with distal and proximal colon cancer were 24.5%and18.6%,respectively.Patients with rectal cancer were predominantly male and were the youngest for all anatomical sites(each P<0.001).The highest proportion of emergency admissions,the longest hospital stays and the highest hospitalization costs were found in patients with proximal colon cancer(each P<0.001).The proximal colon cancer subgroup included the highest proportions of patients with medical histories of cholecystectomy,cholecystolithiasis and/or gallbladder polyps and appendectomy(P=0.009,P<0.001 and P<0.001,respectively).The distal colon cancer subgroup included the highest proportions of patients with medical histories of diabetes and hypertension(P<0.001,respectively).Conclusions:The patterns of colorectal cancer observed in this study differ from those reported for Western patients and show a significantly higher proportion of patients with rectal cancer.Different epidemiological features were also found based on anatomic sites.Further studies based on tumor location should be conducted to facilitate more accurate screening and treatment. | Ruize Qu Yanpeng Ma Liyuan Tao Xiaoyuan Bao Xin Zhou Bingyan Wang Fei Li Siyi Lu Lin Tuo Siyan Zhan Zhipeng Zhang Wei Fu | 2021 | Chinese Journal of Cancer Research2021，33，4： | 10 |
| 9 | Wheat genomic study for genetic improvement of traits in China显示文摘Bread wheat(Triticum aestivum L.)is a major crop that feeds 40%of the world’s population.Over the past several decades,advances in genomics have led to tremendous achievements in understanding the origin and domestication of wheat,and the genetic basis of agronomically important traits,which promote the breeding of elite varieties.In this review,we focus on progress that has been made in genomic research and genetic improvement of traits such as grain yield,end-use traits,flowering regulation,nutrient use efficiency,and biotic and abiotic stress responses,and various breeding strategies that contributed mainly by Chinese scientists.Functional genomic research in wheat is entering a new era with the availability of multiple reference wheat genome assemblies and the development of cutting-edge technologies such as precise genome editing tools,highthroughput phenotyping platforms,sequencing-based cloning strategies,high-efficiency genetic transformation systems,and speed-breeding facilities.These insights will further extend our understanding of the molecular mechanisms and regulatory networks underlying agronomic traits and facilitate the breeding process,ultimately contributing to more sustainable agriculture in China and throughout the world. | Jun Xiao Bao Liu Yingyin Yao Zifeng Guo Haiyan Jia Lingrang Kong Aimin Zhang Wujun Ma Zhongfu Ni Shengbao Xu Fei Lu Yuannian Jiao Wuyun Yang Xuelei Lin Silong Sun Zefu Lu Lifeng Gao Guangyao Zhao Shuanghe Cao Qian Chen Kunpu Zhang Mengcheng Wang Meng Wang Zhaorong Hu Weilong Guo Guoqiang Li Xin Ma Junming Li Fangpu Han Xiangdong Fu Zhengqiang Ma Daowen Wang Xueyong Zhang Hong-Qing Ling Guangmin Xia Yiping Tong Zhiyong Liu Zhonghu He Jizeng Jia Kang Chong | 2022 | Science China(Life Sciences)2022，65，9： | 9 |
| 10 | Long-Term Cognitive Improvement After Benfotiamine Administration in Patients with Alzheimer's Disease显示文摘To date,we still lack disease-modifying therapies for Alzheimer's disease(AD).Here,we report that long-term administration of benfotiamine improved the cognitive ability of patients with AD.Five patients with mild to moderate AD received oral benfotiamine(300 mg daily) over 18 months.All patients were examined by positron emission tomography with Pittsburgh compound B(PiB-PET) and exhibited positive imaging with pamyloid deposition,and three received PiB-PET imaging at follow-up.The five patients exhibited cognitive improvement as assayed by the Mini-Mental Status Examination(MMSE) with an average increase of 3.2 points at month18 of benfotiamine administration.The three patients who received follow-up PiB-PET had a 36.7%increase in the average standardized uptake value ratio in the brain compared with that in the first scan.Importantly,the MMSE scores of these three had an average increase of 3 points during the same period.Benfotiamine significantly improved the cognitive abilities of mild to moderate AD patients independently of brain amyloid accumulation.Our study provides new insight to the development of diseasemodifying therapy. | Xiaoli Pan Zhichun Chen Guoqiang Fei Shumei Pan Weiqi Bao Shuhua Ren Yihui Guan Chunjiu Zhong | 2016 | Neuroscience Bulletin2016，32，6： | 9 |
| 11 | EXPONENTIAL STABILITY OF LINEAR DISTRIBUTED PARAMETER SWITCHED SYSTEMS WITH TIME-DELAY显示文摘In this paper, the exponential stability analysis for ODE switched systems with time delay is extended to distributed parameter switched systems(DPSS) in Hilbert space. For a given family of exponential stable subsystems, this paper focuses on finding conditions to guarantee the overall DPSS' exponential stability. Based on semigroup theory, by applying piecewise Lyapunov-Krasovskii functionals method incorporated average dwell time approach, sufficient conditions for exponential stability are derived. These conditions are given in the form of linear operator inequalities(LOIs)where the decision variables are operators in Hilbert space, and the stability properties depend on switching rule. Being applied to heat switched propagation equations, these LOIs are reduced to standard Linear Matrix Inequalities(LMIs). Finally, a numerical example is given to illustrate the effectiveness of the proposed result. | BAO Leping FEI Shumin YU Lei | 2014 | Journal of Systems Science & Complexity2014，27，2： | 9 |
| 12 | Gold nanoprisms as a hybrid in vivo cancer theranostic platform for in situ photoacoustic imaging, angiography, and localized hyperthermia显示文摘高分辨率的 nanosized photoacoustic 对比代理人的发展是令人激动的还挑战性的工艺的进展。此处,抗体(胸联系癌症的抗原 1 (Brcaa1 ) monoclonal 抗体)- 并且肽(RGD )-functionalized 金牌 nanoprisms (AuNprs ) 被用作组合方法论为在使用的 situ photoacoustic 成像, angiography,和局部性的过高热, orthotopic 和下的鼠科的胃的癌当模特儿。结合 RGD 的 PEGylated AuNprs 为结合抗体的 PEGylated AuNprs 被用于指向的肿瘤 angiography,和 Brcaa1 monoclonal 并且为是可得到的在在 orthotopic 的胃的癌的 situ 成像,肿瘤当模特儿。在 situ photoacoustic 成像允许在肿瘤地点的解剖、功能的成像。在 vivo 肿瘤 angiography,成像以一种时间依赖者方式显示出 photoacoustic 信号的改进。而且, photoacoustic 成像证明肿瘤容器清楚地在局部性的过高热以后被损坏。这作为高度敏感的对比和治疗学的代理人是用二根 AuNprs 探针的第一 proof-of-concept 为在 situ 肿瘤察觉和抑制。这些聪明的抗体 / 肽 AuNprs 能被用作一个有效 nanotheranostic 平台为在局部性的过高热处理以后在有高敏感的 vivo 肿瘤察觉,以及为指向治疗,与单个剂量的注射,它导致肿瘤的肿瘤缩放减小和增加老鼠幸存。 | Chenchen Bao Joao Conde Fei Pan Chao Li Chunlei Zhang Furong Tian Shujing Liang Jesus M. de la Fuente Daxiang Cui | 2016 | Nano Research2016，9，4： | 9 |
| 13 | Association between Plasma Metal Levels and Diabetes Risk: a Case-control Study in China显示文摘Objective Many metals, some of which have been classified as environmental endocrine disruptors, are used extensively in everyday consumer products and are ubiquitous in our living environment. In the present study, we aimed to explore the associations between the prevalence risk of type 2 diabetes and plasma levels of 20 trace elements as well as those of heavy metals in a Han Chinese population. Methods We conducted a case-control study to investigate the associations between plasma concentrations of 20 metals and diabetes in Jiangsu province. A total of 122 newly diagnosed cases of type 2 diabetes and 429 matched controls were recruited from community physical examinations in Suzhou City of Jiangsu Province. Plasma metal levels were measured by inductively-coupled plasma mass spectrometry. Results After adjusting for confounders, plasma vanadium, chromium, manganese, copper, zinc, arsenic, selenium, strontium, palladium, cadmium, cesium, and barium were associated with diabetes risk(P < 0.05). The adjusted OR increased with increasing concentration of vanadium, manganese, copper, zinc, and cesium. Conclusion Many metals, including manganese, copper, zinc, arsenic, selenium, and cadmium in plasma, are associated with the morbidity of diabetes. Monitoring of environmental metal levels and further studies are urgently needed. | LI Xiu Ting YU Peng Fei GAO Yan GUO Wen Hui WANG Jun LIU Xin GU Ai Hua JI Gui Xiang DONG Qiu WANG Bo Shen CAO Ying ZHU Bao Li XIAO Hang | 2017 | Biomedical and Environmental Sciences2017，30，7： | 8 |
| 14 | The Theory of Biomedical Knowledge Integration (Ⅶ)──The Non-Euclid Macro-Micro Transform Law显示文摘This article continued to do the scholastic pursuits on some profound mechanisms in the life systems, which are believed to be related to the further development of Medical Informatics. It discussed at first the structural nature of things, then probed a principle which is a basis for both of the fractal theory and the wavelet analysis, being called the shape-constancy law of the basic constructors at the different scale levels. And the paper also ventured the equivalency between the shape of wave and matrix, thus presented a new concept “shaped-number”, being expected to work in the operations of some bio-medical functions or shapes. | BAO Han - fei | 2006 | 医学信息(西安上半月)2006，19，11： | 8 |
| 15 | Prophylactic vertebroplasty procedure applied with a resorbable bone cement can decrease the fracture risk of sandwich vertebrae:long-term evaluation of clinical outcomes显示文摘A sandwich vertebra is formed after multiple osteoporotic vertebral fractures treated by percutaneous vertebroplasty,which has a risk of developing new fractures.The purpose of our study was to(i)investigate the occurrence of new fractures in sandwich vertebra after cement augmentation procedures and to(ii)evaluate the clinical outcomes after prophylactic vertebral reinforcement applied with resorbable bone cement.From June 2011 to 2014,we analysed 55 patients with at least one sandwich vertebrae and treated with percutaneous vertebroplasty.Eighteen patients were treated by prophylactic vertebroplasty with a resorbable bone cement to strengthen the sandwich vertebrae as the prevention group.The others were the non-prevention group.All patients were examined by spinal radiographs within 1 day,6 months,12 months,24 months and thereafter.The incidence of sandwich vertebra is 8.25%(55/667)in our study.Most sandwich vertebrae(69.01%,49/71)are distributed in the thoracic-lumbar junction.There are 24 sandwich vertebrae(18 patients)and 47 sandwich vertebrae(37 patients)in either prevention group or non-prevention group,respectively.No significant difference is found between age,sex,body mass index,bone mineral density,cement disk leakage,sandwich vertebrae distribution or Cobb angle in the two groups.In the follow-up,8 out of 37(21.6%)patients(with eight sandwich vertebrae)developed new fractures in non-prevention’group,whereas no new fractures were detected in the prevention group.Neither Cobb angle nor vertebral compression rate showed significant change in the prevention group during the follow-up.However,in the non-prevention group,we found that Cobb angle increased and vertebral height lost significantly(P<0.05).Prophylactic vertebroplasty procedure applied with resorbable bone cement could decrease the rate of new fractures of sandwich vertebrae. | Pu Jia Hai Tang Hao Chen Li Bao Fei Feng He Yang Jinjun Li | 2017 | Regenerative Biomaterials2017，4，1： | 8 |
| 16 | Influence of Low Load Truncation Level on Crack Growth for Al 2324-T39 and Al 7050-T7451显示文摘Tests with middle-crack tension(M(T)) specimens made of Al 2324-T39 and Al 7050-T7451 are conducted to investigate the influence of low load truncation level on fatigue crack growth.The six different truncated spectra are obtained by removing the small cycles of which amplitudes are less than the specified percentages of the maximum amplitude in the basic flight-by-flight loading spectrum and the remainder of the spectrum is untouched.The tests indicate that the mean level of fatigue crack growth life(FCGL) increases as the load truncation level is enhanced.Considering both the time saving and the influence on FCGL,there is an applicable choice(i.e.spectrum S2 or spectrum S3 in this investigation) for full scale fatigue test.The scatter of FCGL becomes much larger than that under the basic spectrum when the load truncation level is increased to a specified high level,mainly due to the occurrence of crack slanting and branching under the high level truncated loading spectra. | Tian Hailing Bao Rui Zhang Jianyu Zheng Xiaoling Fei Binjun | 2009 | Chinese Journal of Aeronautics2009，22，4： | 8 |
| 17 | Whole Genome Analysis Reveals New Insights into Macrolide Resistance in Mycoplasma pneumoniae显示文摘Objective Mutations in 23 S rRNA gene are known to be associated with macrolide resistance in Mycoplasma pneumoniae(M. pneumoniae). However, these mutations alone do not fully explain the high resistance rates in Asia. The aim of this study was to investigate other possible mutations involved in macrolide resistance in M. pneumoniae. Methods The whole genomes of 10 clinical isolates of M. pneumoniae with macrolide resistance were sequenced by Illumina Hi Seq2000 platform. The role of the macrolide-specific efflux transporter was assessed by efflux-pump inhibition assays with reserpine and carbonyl cyanide m-chlorophenyl-hydrazone(CCCP). Results A total of 56 single nucleotide polymorphisms(SNPs) were identified in 10 clinical isolates in comparison to the reference strains M129 and FH. Strikingly, 4 of 30 SNPs causing non-synonymous mutations were clustered in macrolide-specific efflux system gene mac B encoding macrolide-specific efflux pump protein of the ATP-binding cassette transporter family. In assays of the minimal inhibitory concentrations(MIC) of macrolide antibiotics in the presence of the efflux pump inhibitors caused a significant decrease of MICs, even under detectable levels in some strains. Conclusion Our study suggests that macrolide efflux pump may contribute to macrolide resistance in M. pneumoniae in addition to the common point mutations in 23 S r RNA gene. | LI Shao Li SUN Hong Mei ZHU Bao Li LIU Fei ZHAO Han Qing | 2017 | Biomedical and Environmental Sciences2017，30，5： | 7 |
| 18 | The USDA cucumber (Cucumis sativus L.) collection: genetic diversity, population structure, genome-wide association studies, and core collection development显示文摘Germplasm collections are a crucial resource to conserve natural genetic diversity and provide a source of novel traits essential for sustained crop improvement.Optimal collection,preservation and utilization of these materials depends upon knowledge of the genetic variation present within the collection.Here we use the high-throughput genotyping-by-sequencing(GBS)technology to characterize the United States National Plant Germplasm System(NPGS)collection of cucumber(Cucumis sativus L.).The GBS data,derived from 1234 cucumber accessions,provided more than 23 K high-quality single-nucleotide polymorphisms(SNPs)that are well distributed at high density in the genome(~1 SNP/10.6 kb).The SNP markers were used to characterize genetic diversity,population structure,phylogenetic relationships,linkage disequilibrium,and population differentiation of the NPGS cucumber collection.These results,providing detailed genetic analysis of the U.S.cucumber collection,complement NPGS descriptive information regarding geographic origin and phenotypic characterization.We also identified genome regions significantly associated with 13 horticulturally important traits through genome-wide association studies(GWAS).Finally,we developed a molecularly informed,publicly accessible core collection of 395 accessions that represents at least 96%of the genetic variation present in the NPGS.Collectively,the information obtained from the GBS data enabled deep insight into the diversity present and genetic relationships among accessions within the collection,and will provide a valuable resource for genetic analyses,gene discovery,crop improvement,and germplasm preservation. | Xin Wang Kan Bao Umesh K.Reddy Yang Bai Sue A.Hammar Chen Jiao Todd C.Wehner Axel O.Ramírez-Madera Yiqun Weng Rebecca Grumet Zhangjun Fei | 2018 | Horticulture Research2018，5，1： | 7 |
| 19 | Depression of the Thoracolumbar Posterior Vertebral Body on the Estimation of Cement Leakage in Vertebroplasty and Kyphoplasty Operations显示文摘 | Hao Chen Pu Jia Li Bao Fei Feng He Yang Jin-Jun Li Hai Tang | 2015 | Chinese Medical Journal2015，，23： | 7 |
| 20 | Protective efficacy of an H5/H7 trivalent inactivated vaccine(H5-Re13,H5-Re14, and H7-Re4 strains) in chickens, ducks, and geese against newly detected H5N1, H5N6, H5N8, and H7N9 viruses显示文摘Some H5 viruses isolated in poultry or wild birds between 2020 and 2021 were found to be antigenically different from the vaccine strains(H5-Re11 and H5-Re12) used in China. In this study, we generated three new recombinant vaccine seed viruses by using reverse genetics and used them for vaccine production. The vaccine strain H5-Re13 contains the hemagglutinin(HA) and neuraminidase(NA) genes of an H5 N6 virus that bears the clade 2.3.4.4 h HA gene, H5-Re14 contains the HA and NA genes of an H5 N8 virus that bears the clade 2.3.4.4 b HA gene, and H7-Re4 contains the HA and NA genes of H7 N9 virus detected in 2021. We evaluated the protective efficacy of the novel H5/H7 trivalent inactivated vaccine in chickens, ducks, and geese. The inactivated vaccine was immunogenic and induced substantial antibody responses in the birds tested. Three weeks after vaccination, chickens were challenged with five different viruses detected in 2020 and 2021: three viruses(an H5 N1 virus, an H5 N6 virus, and an H5 N8 virus) bearing the clade 2.3.4.4 b HA gene, an H5 N6 virus bearing the clade 2.3.4.4 h HA gene, and an H7 N9 virus. All of the control birds shed high titers of virus and died within 4 days post-challenge, whereas the vaccinated chickens were completely protected from these viruses. Similar protective efficacy against H5 viruses bearing the clade 2.3.4.4 h or 2.3.4.4 b HA gene was observed in ducks and geese. Our study indicates that the newly updated H5/H7 vaccine can provide solid protection against the H5 and H7 N9 viruses that are currently circulating in nature. | ZENG Xian-ying HE Xin-wen MENG Fei MA Qi WANG Yan BAO Hong-mei LIU Yan-jing DENG Guo-hua SHI Jian-zhong LI Yan-bing TIAN Guo-bin CHEN Hua-lan | 2022 | Journal of Integrative Agriculture2022，21，7： | 7 |
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