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| 1 | Transplantation of collagen scaffold with autologous bone marrow mononuclear cells promotes functional endometrium reconstruction via downregulating ΔNp63 expression in Asherman's syndrome显示文摘Asherman's syndrome(AS) is a common disease that presents endometrial regeneration disorder. However, little is known about its molecular features of this aregenerative endometrium in AS and how to reconstruct the functioning endometrium for the patients with AS. Here, we report that ΔNp63 is significantly upregulated in residual epithelial cells of the impaired endometrium in AS; the upregulated-ΔNp63 induces endometrial quiescence and alteration of stemness. Importantly, we demonstrate that engrafting high density of autologous bone marrow mononuclear cells(BMNCs) loaded in collagen scaffold onto the uterine lining of patients with AS downregulates ΔNp63 expression, reverses ΔNp63-induced pathological changes, normalizes the stemness alterations and restores endometrial regeneration. Finally, five patients achieved successful pregnancies and live births. Therefore, we conclude that ΔNp63 is a crucial therapeutic target for AS. This novel treatment significantly improves the outcome for the patients with severe AS. | Guangfeng Zhao Yun Cao Xianghong Zhu Xiaoqiu Tang Lijun Ding Haixiang Sun Juan Li Xinan Li Chenyan Dai Tong Ru Hui Zhu Jingjie Lu Caimei Lin Jingmei Wang Guijun Yan Huiyan Wang Lei Wang Yimin Dai Bin Wang Ruotian Li Jianwu Dai Yan Zhou Yali Hu | 2017 | Science China(Life Sciences)2017，60，4： | 39 |
| 2 | Collagen-binding basic fibroblast growth factor improves functional remodeling of scarred endometrium in uterine infertile women: a pilot study显示文摘Intrauterine adhesion(IUA) is a common cause of uterine infertility and one of the most severe clinical features is endometrial fibrosis namely endometrial scarring for which there are few cures currently. Blocked angiogenesis is the main pathological change in the scarred endometrium. The fibroblast growth factor 2(b FGF), a member of FGF family, is usually applied to promote healing of refractory ulcer and contributes to angiogenesis of tissues. In this study, the sustained-release system of b FGF100 μg was administrated around scarred endometrium guiding by ultrasound every 4 weeks in 18 patients(2–4 times). Results showed that after treatment, the menstrual blood volume, endometrial thickness and the scarred endometrial area were improved.Histological study showed blood vessel density increased obviously. Three patients(3/18) achieved pregnancy over 20 gestational weeks. Therefore, administrating the b FGF surrounding scarred endometrium may provide a new therapeutic approach for the patients with endometrial fibrosis. | Peipei Jiang Xiaoqiu Tang Huiyan Wang Chenyan Dai Jing Su Hui Zhu Minmin Song Jingyu Liu Ziqing Nan Tong Ru Yaling Li Jingmei Wang Jun Yang Bing Chen Jianwu Dai Yali Hu | 2019 | Science China(Life Sciences)2019，62，12： | 16 |
| 3 | No tillage outperforms conventional tillage under arid conditions and following fertilization显示文摘Reduced tillage practices present a tool that could sustainably intensify agriculture.The existing literature,however,lacks a consensus on how and when reduced tillage practices should get implemented.We reanalyzed here an extensive dataset comparing how regular tillage practices(i.e.,conventional tillage)impacted yield of eight crops compared to stopping tillage altogether(i.e.,no-tillage practice).We observed that aridity and fertilization favored no tillage over conventional tillage whereas conventional tillage performed better under high fertility settings.We further show that the responses are consistent across the crops.Our reanalysis complements the original and fills a gap in the literature questioning the conditions under which reducing tillage presents a viable alternative to common tillage practices. | Stavros D.Veresoglou Junjiang Chen Xuheng Du Qi Fu QingLiu Geng Chenyan Huang Xilin Huang Nan Hu Yiming Hun Guolin C.Li Zhiman Lin Zhiyu Ma Yuyi Ou Shuo Qi Haitian Qin Yingbo Qiu Xibin Sun Ye Tao YiLing Tian Jie Wang Lingxiao Wu Ziwei Wu Siqi Xie Ao Yang Dan Yang Chen Zeng Ying Zeng RuJie Zhang | 2023 | Soil Ecology Letters2023，5，1： | 1 |
| 4 | Effect of autologous bone marrow stem cells-scaffold transplantation on the ongoing pregnancy rate in intrauterine adhesion women:a randomized,controlled trial显示文摘Intrauterine adhesion is a major cause of female reproductive disorders.Although we and others uncontrolled pilot studies showed that treatment with autologous bone marrow stem cells made a few patients with severe intrauterine adhesion obtain live birth,no large sample randomized controlled studies on this therapeutic strategy in such patients have been reported so far.To verify if the therapy of autologous bone marrow stem cells-scaffold is superior to traditional treatment in moderate to severe intrauterine adhesion patients in increasing their ongoing pregnancy rate,we conducted this randomized controlled clinical trial.Totally 195 participants with moderate to severe intrauterine adhesion were screened and 152 of them were randomly assigned in a 1:1 ratio to either group with autologous bone marrow stem cells-scaffold plus Foley balloon catheter or group with only Foley balloon catheter(control group)from February 2016 to January 2020.The per-protocol analysis included 140 participants:72 in bone marrow stem cells-scaffold group and 68 in control group.The ongoing pregnancy occurred in 45/72(62.5%)participants in the bone marrow stem cells-scaffold group which was significantly higher than that in the control group(28/68,41.2%)(RR=1.52,95%CI 1.08–2.12,P=0.012).The situation was similar in live birth rate(bone marrow stem cells-scaffold group 56.9%(41/72)vs.control group 38.2%(26/68),RR=1.49,95%CI 1.04–2.14,P=0.027).Compared with control group,participants in bone marrow stem cells-scaffold group showed more menstrual blood volume in the 3rd and 6th cycles and maximal endometrial thickness in the 6th cycle after hysteroscopic adhesiolysis.The incidence of mild placenta accrete was increased in bone marrow stem cells-scaffold group and no severe adverse effects were observed.In conclusion,transplantation of bone marrow stem cells-scaffold into uterine cavities of the participants with moderate to severe intrauterine adhesion increased their ongoing pregnancy and live birth rates,and this therapy was relatively safe. | Hui Zhu Taishun Li Peizhen Xu Lijun Ding Xianghong Zhu Bin Wang Xiaoqiu Tang Juan Li Pengfeng Zhu Huiyan Wang Chenyan Dai Haixiang Sun Jianwu Dai Yali Hu | 2024 | Science China(Life Sciences)2024，67，1： | 0 |
| 5 | Exposure to methylparaben at environmentally realistic concentrations significantly impairs neuronal health in adult zebrafish显示文摘Methylparaben(MeP)is an emerging aquatic pollutant that is found to impact neural functions.However,it still lacks a comprehensive understanding about its neurotoxicology.The present study exposed adult zebrafish to environmentally realistic concentrations(0,1,3,and 10μg/L)of MeP for 28 days,with objectives to elucidate the neurotoxic effects andmechanisms.Proteomic profiling found that MeP pollutant induced distinct mechanism of neurotoxicity as a function of sex.MeP pollutant appeared to preferentially target the neurotransmission cascade via synapse junctions.In male brain,glutamatergic neural signaling was enhanced by 10μg/L of MeP in characteristics of higher glutamate neurotransmitter content(by 61.9%)and up-regulated glutamate receptor expression by 2.6-fold relative to the control.In MeP-exposed female brain,biomarker proteins of synapse formation and regeneration had significantly lower abundance,accounting for the blockage of synaptic neurotransmission.Furthermore,under the stress of MeP pollutant,both male and female zebrafish initiated a negative feedback mechanism along stress neuroendocrine axis by down-regulating the transcriptions of corticotropin-releasing hormone and its binding protein,which subsequently decreased blood cortisol concentrations.MeP subchronic exposure also disturbed innate immune function.In particular,significant increases in lipopolysaccharide(LPS)content by 15.6%were caused by MeP exposure in male brain,thereby inducing the synthesis of pro-inflammatory cytokines.In contrast,female brain was able to adaptively up-regulate the protein expression of blood brain barrier to inhibit the infiltration of LPS endotoxin into brain.Overall,the present findings pinpoint the potent neurotoxicity of MeP pollutant even at environmentally realistic concentrations. | Chenyan Hu Yachen Bai Baili Sun Xiangzhen Zhou Lianguo Chen | 2023 | Journal of Environmental Sciences2023，，10： | 0 |
| 6 | Glucose-responsive,antioxidative HA-PBA-FA/EN106 hydrogel enhanced diabetic wound healing through modulation of FEM1b-FNIP1 axis and promoting angiogenesis显示文摘The diabetic wounds remain to be unsettled clinically,with chronic wounds characterized by drug-resistant bacterial infections,compromised angiogenesis and oxidative damage to the microenvironment.To ameliorate oxidative stress and applying antioxidant treatment in the wound site,we explore the function of folliculininteracting protein 1(FNIP1),a mitochondrial gatekeeper protein works to alter mitochondrial morphology,reduce oxidative phosphorylation and protect cells from unwarranted ROS accumulation.And our in vitro experiments showed the effects of FNIP1 in ameliorating oxidative stress and rescued impaired angiogenesis of HUVECs in high glucose environment.To realize the drug delivery and local regulation of FNIP1 in diabetic wound sites,a novel designed glucose-responsive HA-PBA-FA/EN106 hydrogel is introduced for improving diabetic wound healing.Due to the dynamic phenylboronate ester structure with a phenylboronic acid group between hyaluronic acid(HA)and phenylboronic acid(PBA),the hydrogel is able to realize a glucose-responsive release of drugs.Fulvic acid(FA)is added in the hydrogel,which not only severs as crosslinking agent but also provides antibacterial and anti-inflammatory abilities.Moreover,the release of FEM1b-FNIP1 axis inhibitor EN106 ameliorated oxidative stress and stimulated angiogenesis through FEM1b-FNIP1 axis regulation.These in vivo and in vitro results demonstrated that accelerated diabetic wounds repair with the use of the HA-PBA-FA/EN106 hydrogel,which may provide a promising strategy for chronic diabetic wound repair. | Wenqian Zhang Kangkang Zha Yuan Xiong Weixian Hu Lang Chen Ze Lin Chenyan Yu Wu Zhou Faqi Cao Hankun Hu Bobin Mi Guohui Liu | 2023 | Bioactive Materials2023，，12： | 0 |
| 7 | Variability in fecal metabolome depending on age, PFBS pollutant, and fecal transplantation in zebrafish: A non-invasive diagnosis of health显示文摘To protect the wellbeing of research animals,certain non-invasive measures are in increasing need to facilitate an early diagnosis of health and toxicity.In this study,feces specimen was collected from adult zebrafish to profile the metabolome fingerprint.Variability in fecal metabolite composition was also distinguished as a result of aging,perfluorobutanesulfonate(PFBS)toxicant,and fecal transplantation.The results showed that zebrafish feces was very rich in a diversity of metabolites that belonged to several major classes,including lipid,amino acid,carbohydrate,vitamin,steroid hormone,and neurotransmitter.Fecal metabolites had functional implications to multiple physiological activities,which were characterized by the enrichment of digestion,absorption,endocrine,and neurotransmission processes.The high richness and functional involvement of fecal metabolites pinpointed feces as an abundant source of diagnostic markers.By comparison between young and aged zebrafish,fundamental modifications of fecal metabolomes were caused by aging progression,centering on the neuroactive ligand-receptor interaction pathway.Exposure of aged zebrafish to PFBS pollutant also significantly disrupted the metabolomic structure in feces.Of special concern were the changes in fecal hormone intermediates after PFBS exposure,which was concordant with the in vivo endocrine disrupting effects of PFBS.Furthermore,itwas intriguing that transplantation of young zebrafish feces efficientlymitigated the metabolic perturbation of PFBS in aged recipients,highlighting the health benefits of therapeutic strategies based on gut microbiota manipulation.In summary,the present study provides preliminary clues to evidence the non-invasive advantage of fecal metabolomics in the early diagnosis and prediction of physiology and toxicology. | Baili Sun Mengyuan Liu Lizhu Tang Xiangzhen Zhou Chenyan Hu Lianguo Chen | 2023 | Journal of Environmental Sciences2023，，5： | 0 |
| 8 | Diagnosis and Treatment Guidelines for Mesenchymal Stem Cell Therapy for Coronavirus Disease 2019(Beijing,2021)显示文摘The coronavirus disease 2019(COVID-19)can be caused by severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)infection,and has led to millions of deaths among more than 100 million infected people around the world according to the declaration from World Health Organization.Dysregulated immune response of both the innate and adaptive immune systems is subsistent on COVID-19 patients,of which the degree are associated with disease severity,lung damage and long term functional disability.Current treatment options have included antiretroviral drugs,anti-inflammatory factors,antibodies,immune checkpoint inhibitors,and convalescent plasma therapy.More recently,mesenchymal stem cell(MSC)therapy has been explored for the management and control of COVID-19,particularly with the aim of preventing or at least mitigating respiratory co-morbidities.Though the safety and efficacy of stem cell therapy have been validated in multiple phase I–III clinical trials,to date,no standardized stem cell preparation,administration dosage or interval,product QA/QC testing,storage,transportation,or disposal protocols have been established.The present paper proposes a systematic methodology that addresses all the foregoing process steps and evaluation criteria for the efficacious and safe administration of MSCs in the treatment of patients infected with COVID-19. | Ruonan Xu Lei Shi Wei-Fen Xie Zhe Xu Fan-Ping Meng Jun-Liang Fu Xin Yuan Lei Huang Ming Shi Yonggang Li Yuanyuan Li Chen Yao Yu Zhang Zhongmin Liu Chenyan Gao Zhaohui Wu Shufang Meng Weidong Han Charlie Xiang Gui-Qiang Wang Taisheng Li Xiaoying Wang Yunxia Sun Zunyou Wu Wenhong Zhang Chunhua Zhao Yu Hu Tao Cheng Yuquan Wei Qi Zhou Fu-Sheng Wang | 2021 | Infectious Diseases & Immunity2021，1，2： | 0 |
| 9 | Fingerprinting fecal DNA and mRNA as a non-invasive strategy to assess the impact of polychlorinated biphenyl 126 exposure on zebrafish显示文摘In toxicological studies,experimental animals are generally subjected to dissection to obtain the tissues of concern,which causes great harm to the animals.In this regard,it is necessary to test and develop a non-invasive strategy to prevent the animals from anthropic injury when achieving scientific objectives.Therefore,zebrafish fecal DNA and mR NA pools were assessed by using metagenomic and transcriptomic analyses based on their potential to diagnose toxicological impairment of polychlorinated biphenyl(PCB)126,a model persistent organic pollutant.The results showed that there was abundant zebrafish DNA and mR NA in the feces,which were,however,associated with contrasting profiles of physiological activities.As compared to DNA fragments,fecal mR NA provided a better representation of zebrafish physiological status.PCB126 exposure dramatically shifted the composition of fecal zebrafish DNA and m RNA as a function of sex.The differential m RNA caused by PCB126 clearly identified the toxicological fingerprint of PCB126.In summary,this study provides preliminary clues about the potential of fecal genes(mRNA in particular)in the development of non-invasive toxicological approaches.In the future,it is expected that more works will be conducted to screen sensitive diagnostic biomarkers from feces to increase the rate and reduce the cost of ecological risk assessment. | Mengyuan Liu Yumiao Sun Lizhu Tang Chenyan Hu Baili Sun Zileng Huang Lianguo Chen | 2021 | Journal of Environmental Sciences2021，33，8： | 0 |
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