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| 1 | A complete sequence and comparative analysis of a SARS-associated virus(Isolate BJ01)显示文摘The genome sequence of the Severe Acute Respiratory Syndrome (SARS)-associated virus provides essential information for the identification of pathogen(s), exploration of etiology and evolution, interpretation of transmission and pathogenesis, development of diagnostics, prevention by future vaccination, and treatment by developing new drugs. We report the complete genome sequence and comparative analysis of an isolate (BJ01) of the coronavirus that has been recognized as a pathogen for SARS. The genome is 29725 nt in size and has 11 ORFs (Open Reading Frames). It is composed of a stable region encoding an RNA-dependent RNA polymerase (composed of 2 ORFs) and a variable region representing 4 CDSs (coding sequences) for viral structural genes (the S, E, M, N proteins) and 5 PUPs (putative uncharacterized proteins). Its gene order is identical to that of other known coronaviruses. The sequence alignment with all known RNA viruses places this virus as a member in the family of Coronaviridae. Thirty putative substitutions have been identified by comparative analysis of the 5 SARS- associated virus genome sequences in GenBank. Fifteen of them lead to possible amino acid changes (non-synonymous mutations) in the proteins. Three amino acid changes, with predicted alteration of physical and chemical features, have been detected in the S protein that is postulated to beinvolved in the immunoreactions between the virus and its host. Two amino acid changes have been detected in the Mprotein, which could be related to viral envelope formation. Phylogenetic analysis suggests the possibility of non-human origin of the SARS-associated viruses but provides noevidence that they are man-made. Further efforts should focus on identifying the etiology of the SARS-associated virus and ruling out conclusively the existence of otherpossible SARS-related pathogen(s). | QIN E'de ZHU Qingyu YU Man FAN Baochang CHANG Guohui SI Bingyin YANG Bao PENG Wenming JIANG Tao LIU Bohua DENG Yongqiang LIU Hong ZHANG Yu WANG Cui LI Yuquan GAN Yonghua LI Xiaoyu L Fushuang TAN Gang CAO Wuchun, YANG Ruifu Institute of Microbiology and Epidemiology, Chinese Academy of Military Medical Sciences, Beijing 100071, China WANG Jian, LI Wei, XU Zuyuan, LI Yan, WU Qingfa, LIN Wei, CHEN Weijun, TANG Lin, DENG Yajun, HAN Yujun, LI Changfeng, LEI Meng, LI Guoqing, LI Wenjie, L Hong, SHI Jianping, TONG Zongzhong, ZHANG Feng, LI Songgang, LIU Bin, LIU Siqi, DONG Wei, WANG Jun, Gane K-S Wong, YU Jun & YANG Huanming* Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300 National Center for Genome Information, Beijing 101300, China | 2003 | Chinese Science Bulletin2003，48，10： | 121 |
| 2 | EXPERIMENTAL STUDY OF FLOWFIELD STRUCTURE AROUND AN OGIVE-CYLINDER显示文摘The flowfield structure and their aerodynamic characteristics over an ogive cylinder were studied by means of flow visualization and surface pressure measurement in a water tunnel and a wind tunnel. The existence of multi asymmetric vortices over long slender bodies was experimentally confirmed at large angles of attack and in the subcritical Reynolds number range. The spatial 3 D characteristics of the multi vortices system were analyzed and a physical model was developed. The topological structure of different patterns in cross flow plane was studied and the mechanism governing the formation of asymmetric vortices and multi vortices was discussed from the viewpoint of stability of the topological structure. It was concluded that the maximum in the sectional side force distribution curve are not caused by the shedding of higher position vortex, but by the cross over to the symmetric plane of the lower position vortex. | Ph.D.Candidate:Wang Gang Fluid Mechanics Institute, Beijing University of Aeronautics and Astronautics, Beijing 100083, China,Supervisor: Deng Xue ying (Beijing University of Aeronautics and Astronautics) Members of Dissertation Defense Committee: Tong Bing gang (Graduate School of the Chinese Academy of Sciences),Chairman Gu Zhi fu (Peking University) Yang Qi de (China Aerodynamics Research and Development Center) Lv Zhi yong (Beijing University of Aeronautics and Astronautics) Liu Pei qing (Beijing University of Aeronautics and Astronautics) | 2003 | Journal of Hydrodynamics2003，15，5： | 18 |
| 3 | MALE GAMETOPHYTE DEFECTIVE 2,Encoding a Sialyltransferase-like Protein,is Required for Normal Pollen Germination and Pollen Tube Growth in Arabidopsis显示文摘Sialyltransferases(SiaTs) exist widely in vertebrates and play important roles in a variety of biological processes.In plants,several genes have also been identified to encode the proteins that share homology with the vertebrate SiaTs.However,very little is known about their functions in plants.Here we report the identification and characterization of a novel Arabidopsis gene,MALE GAMETOPHYTE DEFECTIVE 2(MGP2) that encodes a sialyltransferase-like protein.MGP2 was expressed in all tissues including pollen grains and pollen tubes.The MGP2 protein was targeted to Golgi apparatus.Knockout of MGP2 significantly inhibited the pollen germination and retarded pollen tube growth in vitro and in vivo,but did not affect female gametophytic functions.These results suggest that the sialyltransferase-like protein MGP2 is important for normal pollen germination and pollen tube growth,giving a novel insight into the biological roles of the sialyltransferase-like proteins in plants. | Yi Deng Wei Wang Wen-Qing Li Chuan Xia Hong-Ze Liao Xue-Qin Zhang De Ye | 2010 | Journal of Integrative Plant Biology2010，52，9： | 7 |
| 4 | Experimental study on the mechanical properties and consolidation mechanism of microbial grouted backfill显示文摘Backfill mining technology is the practice of returning waste materials underground for both disposal and geotechnical stability,however,a challenge with current technologies is that they commonly require cement-based binders which have a relatively high environmental impact.Finding alternatives to cement-based binders can improve environmental performance and this paper proposes microbial grouted backfill(MGB)as a potential solution.In this paper,the effects of the cementation solution concentration(CSC),volume ratio of bacterial solution to cementation solution(VRBC),particle sizes of the aggregates,and the number of grouting batches on the mechanical properties of MGB are studied.The experimental results show that MGB strength increased,up to a peak value,as CSC was increased,before decreasing as CSC was increased further.The results also show that MGB strength increased,up to a peak value,as VRBC decreased,before decreasing as the VRBC was decreased further.The peak strength was achieved at a CSC of 2 mol/L and a VRBC of 1:9.The strength of the MGB also increased as the number of grouting batches increased.Graded MGB samples showed the highest UCS,25.12 MPa,at particle sizes of 0.2 to 0.8 mm,while full(non-graded)MGB samples displayed mean UCS values ranging from1.56 MPa when the maximum particle size was 0.2 mm,up to 13 MPa when the maximum particle size was 1.2 mm.MGB samples are consolidated by the calcium carbonate that is precipitated during microbial metabolism,and the strength of MGB increases linearly as calcium carbonate content increases.The calcium carbonate minerals produced in MGB materials are primarily calcite,with secondary amounts of vaterite. | Xuejie Deng Yu Li Fei Wang Xiaoming Shi Yinchao Yang Xichen Xu Yanli Huang Benjamin de Wit | 2022 | International Journal of Mining Science and Technology2022，32，2： | 7 |
| 5 | Compression-related EMIC waves drive relativistic electron precipitation显示文摘With coordinated observations of the NOAA 15 satellite and OUL magnetometer station in Finland, we report that the electromagnetic ion cyclotron(EMIC) waves which were stimulated by the compression of the magnetosphere drive relativistic electron precipitation in geoquiescence on 1 Jan 2007. After an enhancement of solar wind dynamic pressure(SWDP), a dayside Pc1 pulsation was observed by the OUL station. Such a Pc1 pulsation is caused by an EMIC wave which propagates from the generation source to lower altitudes. Simultaneously, the NOAA 15 satellite registered an enhancement of precipitating electron count rates with energies >3 Me V within the anisotropic zone of protons. This phenomenon is coincident with the quasi-linear theoretical calculation presented in this paper. Our observations suggest that after a positive impulse of solar wind, the compression-related EMIC waves can drive relativistic electrons precipitation and play a pivotal role in the dynamic of radiation belts. | WANG De Dong YUAN Zhi Gang DENG Xiao Hua ZHOU Meng HUANG Shi Yong LI Ming LI Hui Min LI Hai Meng RAITA Tero PANG Ye | 2014 | Science China(Technological Sciences)2014，57，12： | 6 |
| 6 | Small ubiquitin-related modifier paralogs are indispensable but functionally redundant during early development of zebrafish显示文摘到许多蛋白质的小 ubiquitin 相关的修饰词(相扑) 变化形式调整多样的细胞的过程,包括抄写,房间周期规定和染色体正直的维护。在 vivo 调查相扑 paralogs 的生物功能,我们在 zebrafish 的早开发使他们失去活性。当作为 Ubc9 缺乏的,为所有三相扑 paralogs 缺乏的 zebrafish 胚胎显示了严重缺点时,单个相扑 paralog 的损失与正常开发兼容。相扑缺乏的胚胎能被一个单个人或 zebrafish 相扑救。当关键时结构的基本离氨酸残余和 N 终端相扑的未组织的段是批评的为在 vivo 营救, SUMO2 的一致 K11 sumoylation 地点是非必需的,在这个潜在的地点上暗示那链形成为正常开发是非本质的。所有三相扑的 Inactivation 触发了 p53 依赖的 apoptosis, p53 的进一步的 inactivation 恢复了正常 zebrafish 开发。有趣地,我们也证明 p53 的主导的否定截断的形式,螖 1 13p53,显著地弄钝相扑在 vivo 的导致弄空的 p53 活动。一起拿,我们的结果建议相扑 paralogs 不可缺少,却冗余,在 zebrafish 的早发展。 | Hao Yuan Jun Zhou Min Deng Xi Liu Morgane Le Bras Hugues de The Sai Juan Chen Zhu Chen Ting Xi Liu Jun Zhu | 2010 | Cell Research2010，20，2： | 4 |
| 7 | A facile synthesis of 2,4,6-trichloroborazine from boron trichloride-dimethylsulfide complex and ammonium chloride显示文摘2,4,6-Trichloroborazine has been recognized as a desirable monomer for the preparation of high-performance boron nitride fibers through polymer derived ceramics route.So a high yield and facile synthesis of 2,4,6-trichloroborazine is essential in practice. Using boron trichloride-dimethylsulfide complex((CH_3)_2S·BCl_3) and ammonium chloride(NH_4Cl) as starting materials and toluene(C_6H_5CH_3) as solvent,the synthesis of 2,4,6-trichloroborazine to give high yield is reported. | Deng, Cheng Song, Yong Cai Wang, Ying De Li, Yi He Lei, Yong Peng Cao, Shu Wei | 2010 | Chinese Chemical Letters2010，21，2： | 4 |
| 8 | Protective Effect of Procyanidin B2 on Acute Liver Injury Induced by Aflatoxin B1 in Rats显示文摘Objective This study aimed to explore the protective effect of procyanidin B2(PCB2)on acute liver injury induced by aflatoxin B1(AFB1)in rats.Methods Forty Sprague Dawley rats were randomly divided into control,AFB1,AFB1+PCB2,and PCB2 groups.The latter two groups were administrated PCB2 intragastrically(30 mg/kg body weight)for 7 d,whereas the control and AFB1 groups were given the same dose of double distilled water intragastrically.On the sixth day of treatment,the AFB1 and AFB1+PCB2 groups were intraperitoneally injected with AFB1(2 mg/kg).The control and PCB2 groups were intraperitoneally administered the same dose of dimethyl sulfoxide(DMSO).On the eighth day,all rats were euthanized:serum and liver tissue were isolated for further examination.Hepatic histological features were assessed by hematoxylin and eosin-stained sections.Weight,organ coefficient(liver,spleen,and kidney),liver function(serum alanine aminotransferase,aspartate aminotransferase,alkaline phosphatase,total bilirubin,and direct bilirubin),oxidative index(catalase,glutathione,superoxide dismutase,malondialdehyde,and 8-hydroxy-2′-deoxyguanosine),inflammation factor[hepatic interleukin-6(IL-6)m RNA expression and serum IL-6],and bcl-2/bax ratio were measured.Results AFB1 significantly caused hepatic histopathological damage,abnormal liver function,oxidative stress,inflammation,and bcl-2/bax ratio reduction compared with DMSO-treated controls.Our results indicate that PCB2 treatment can partially reverse the adverse liver conditions induced by AFB1.Conclusion Our findings indicate that PCB2 exhibits a protective effect on acute liver injury induced by AFB1. | DENG Zhi Jie ZHAO Jing Fang HUANG Feng SUN Gui Li GAO Wei LU Li XIAO De Qiang | 2020 | Biomedical and Environmental Sciences2020，33，4： | 4 |
| 9 | AEG1通过激活NF-κB和AP-1诱导肝癌HepG2细胞COX-2表达(英文)显示文摘Objective:The aim of this study was to investigate whether astrocyte elevated gene 1 (AEG1) regulates COX-2 expression in human hepatoma HepG2 cells and related pathways involved in this process. Methods:Human hepatoma HepG2 cells were transfected with pcDNA3.1(-)-AEG1 plasmid or psilencer2.0-AEG1-shRNA1 plasmid to up/down-regulate AEG1 expression, pcDNA3.1(-) and psilencer 2.0 empty vector plasmids were transfected respectively as control. Real-time RT-PCR was carried out to measure the expression levels of AEG1 and COX-2 mRNA. The expression levels of AEG1 and COX-2 protein were detected by Western blot. NF-κB signaling was blocked by PDTC, and AP-1 signaling was blocked by curcumin. Results:AEG1 mRNA and protein levels were increased after pcDNA3.1(-)-AEG1 transfection, and decreased after psilencer2.0-AEG1-shRNAs transfection. COX-2 mRNA and protein levels were increased in AEG1-overexpressing cells and decreased in AEG1-knockdown cells. Phosphorylations of p65 and c-jun were up-regulated in AEG1-overexpressing cells. Both PDTC and curcumin reduced COX-2 expression in HepG2 cells with AEG1 overexpression. Conclusion:AEG1-overexpressing and-knockdown HepG2 cells are established successfully. AEG1 could induce COX-2 expression though activating NF-κB and AP-1 in human hepatoma HepG2 cells. | Huan Deng Zhenzhen Zhou Wei Tu Yujia Xia Huanjun Huang De'an Tian | 2013 | The Chinese-German Journal of Clinical Oncology2013，12，6： | 2 |
| 10 | Surface phase defects induced downstream laser intensity modulation in high-power laser facility显示文摘Optics surface phase defects induced intensity modulation in high-power laser facility for inertial confinement fusion research is studied. Calculations and experiments reveal an exact mapping of the modulation patterns and the optics damage spot distributions from the surface phase defects. Origins are discussed during the processes of optics manufacturing and diagnostics, revealing potential improvements for future optics manufacturing techniques and diagnostic index, which is meaningful for fusion level laser facility construction and its operation safety. | Xin Zhang Wei Zhou Wanjun Dai Dongxia Hu Xuewei Deng Wanqing Huang Lidan Zhou Qiang Yuan Xiaoxia Huang De'en Wang Ying Yang | 2016 | High Power Laser Science and Engineering2016，4，1： | 2 |
| 11 | Malaria Surveillance of Entry People During the COVID-19 Epidemic—Guangdong Province,China,October 2020–May 2021显示文摘Summary What is already known about this topic?Malaria control was affected by the coronavirus disease 2019(COVID-19)pandemic.This study conducted active case finding for key flights and key populations to determine malaria transmission.What is added by this report?Surveillance for malaria was conducted for entry personnel coming from areas affected by malaria.It is estimated that at least 100,000 tests were conducted in Guangdong Province. | De Wu Zhuohui Deng Rongxin Lin Qiang Mao Wenchen Lu Caiwen Ruan Yongzhen Cen Ning Xiao Tie Song | 2021 | China CDC weekly2021，3，38： | 2 |
| 12 | Study of the retina algorithm on FPGA for fast tracking显示文摘Real-time track reconstruction in high-energy physics experiments at colliders running at high luminosity is very challenging for trigger systems. To perform pattern recognition and track fitting, artificial retina or Hough transformation algorithms have been introduced to the field typically implemented on state-of-the-art field programmable gate array(FPGA) devices. In this paper, we report on two FPGA implementations of the retina algorithm: one using a mixed Floating-Point core and the other using Fixed-Point and Look-Up Table, and detailed measurements of the retina performance are investigated and compared. So far, the retina has mainly been used in a detector configuration comprising parallel planes, and the goal of our work is to study the hardware implementation of the retina algorithm and estimate the possibility of using such a method in a real experiment. | Zi-Xuan Song Wen-Di Deng Gilles De Lentdecker Guang-Ming Huang Hua Pei Yi-Fan Yang Dong Wang Frederic Robert | 2019 | Nuclear Science and Techniques2019，30，8： | 2 |
| 13 | Fixing, preventing, and recovering from concurrency bugs显示文摘Concurrency bugs are becoming widespread with the emerging ubiquity of multicore processors and multithreaded software. They manifest during production runs and lead to severe losses. Many effective concurrency-bug detection tools have been built. However, the dependability of multi-threaded software does not improve until these bugs are handled statically or dynamically. This article discusses our recent progresses on fixing, preventing, and recovering from concurrency bugs. | DENG DongDong JIN GuoLiang de KRUIJF Marc LI Ang LIBLIT Ben LU Shan QI ShanXiang REN JingLei SANKARALINGAM Karthikeyan SONG LinHai WU YongWei ZHANG MingXing ZHANG Wei ZHENG WeiMin | 2015 | Science China(Information Sciences)2015，58，5： | 2 |
| 14 | 改良FOLFIRINOX方案一线化疗中国转移性胰腺癌患者的疗效与安全性研究显示文摘背景与目的奥沙利铂(oxaliplatin)、伊立替康(irinotecan)、5-氟尿嘧啶(5-fluorouracil)和1-醛氢叶酸(l-leucovorin)联合用药(即FOLFIRINOX方案)已成为晚期胰腺癌(pancreatic cancer,PC)的一线治疗方案之一。然而,与FOLFIRINOX标准剂量相关的3级或4级不良事件的较高发生率限制了该方案在临床上的广泛应用。在本研究中,我们对改良FOLFIRINOX方案一线化疗中国转移性PC患者的疗效和安全性进行了评价。方法将组织学证实为原发转移性胰腺癌,且东部肿瘤协作组(Eastern Cooperative Oncology Group,ECOG)体能状态评分为0-2的患者纳入本研究。所有患者均接受改良FOLFIRINOX方案治疗(静脉滴注奥沙利铂65 mg/m^2、伊立替康150 mg/m^2、l-醛氢叶酸200 mg/m^2、5-氟尿嘧啶2400 mg/m^2,每2周重复1次)。治疗持续12个周期,除非患者出现疾病进展(progressive disease,PD)、症状恶化的疾病稳定(stable disease,SD)、不可耐受的不良事件或要求提前终止治疗。主要终点为客观缓解率(objective response rate,ORR)。结果2012年7月至2017年4月期间,共有来自3个机构的65例患者被纳入本研究。所有患者均接受了至少1个周期化疗,化疗周期中位数为8个周期(范围:1-12个周期)。本研究中未观察到完全缓解。21(32.3%)例患者为部分缓解,27(41.5%)例表现为SD。总体人群的ORR和疾病控制率(diseasecontrol rate,DCR)分别为32.3%和73.8%。中位总生存期和无进展生存期分别为11.60[95%置信区间(confidence interval,CI):8.76-14.44]个月和5.77(95%CI:5.00-6.54)个月。主要的3级或4级不良事件包括中性粒细胞减少(12.3%)和腹泻(6.2%)。未观察到与治疗相关的死亡。结论改良FOLFIRINOX具有良好的耐受性,可作为中国转移性PC中国患者的一线治疗方案。 | Zhi‑Qiang Wang Fei Zhang Ting Deng Le Zhang Fen Feng Feng‑Hua Wang Wei Wang De‑Shen Wang Hui‑Yan Luo Rui‑Hua Xu Yi Ba Yu‑Hong Li | 2019 | 癌症2019，38，8： | 2 |
| 15 | Chloride binding of cement-based materials subjected to external chloride environment – A review显示文摘 | Qiang Yuan Caijun Shi Geert De Schutter Katrien Audenaert Dehua Deng | 2008 | Construction and Building Materials2008，，1： | 2 |
| 16 | Dynamic analysis of rail transit elevated bridge with ladder track显示文摘In this paper,a dynamic analysis model of an elevated bridge with ladder tracks under moving train load is established.The whole process of a train running through an elevated bridge at different speeds is simulated.The dynamic responses of the elevated bridge with ladder track and the running safety and comfort index of train vehicles are evaluated.Compared with the dynamic responses of an elevated bridge with ordinary nonballasted slab track,the ladder track’s effect on reducing the vibration of an elevated bridge is analyzed.The analysis results show that the ladder track has good vibration reduction characteristics as compared to ordinary non-ballasted track. | He XIA Yushu DENG Yongwei ZOU Guido DE ROECK Geert DEGRANDE | 2009 | Frontiers of Structural and Civil Engineering2009，3，1： | 2 |
| 17 | Dengue Fever Outbreaks Caused by Varied Serotype Dengue Virus--Guangdong Province,China,2019显示文摘What is already known about this topic?Dengue fever(DF)outbreaks affect hundreds of millions of people worldwide and have increased significantly in Guangdong Province in 2019.What is added by this report?This paper described briefly DF outbreaks were attributed to several types of dengue virus(DENV)including DENV-1,DENV-2,and DENV-3 in 2019 in Guangdong,tracked the sources of viruses through phylogenetic analysis and epidemiological investigation,and primarily revealed the epidemiological links among the outbreaks.What are the implications for public health practice?The introduction of DENV from DF endemic areas increased pressure on the prevention and control of DF in Guangdong.Early detection of suspected cases and typing and genotyping of circulating viruses should be prioritized and enhanced to promptly assess the likelihood of local transmission,of introduction,and of subsequent sustained local transmission of the virus to implement optimal prevention and control strategies and measures. | De Wu Xin Zhang Aiping Deng Huan Zhang Yintao Zhang Qiqi Tan Zhiqiang Peng Jiandong Li Tie Song | 2020 | China CDC weekly2020，2，38： | 1 |
| 18 | Turbulent Wind Field Simulation of Wind Turbines With Rotational Effects显示文摘为准确得到风轮旋转效应对风力发电机组湍流风速脉动分量的影响,推导了旋转Fourier自谱和互谱。分析了风轮旋转效应通过相干函数和相位延迟体现的物理本质,提出了引入相位延迟参数的旋转Fourier自谱和互谱。以旋转Fourier自谱和互谱的模构建旋转谱矩阵,对其进行Cholesky分解,将相位延迟引入谱表现方法中实现风力发电机组湍流风场的模拟。最后,结合某1.5Mw三叶片变桨距风电机组进行纵向脉动风速时程的数值模拟,并对计算功率谱与目标谱进行了校核,证明该文算法可以更为准确地模拟作用在风轮旋转叶片上的脉动风速时程。 | HE Wei TIAN De DENG Ying WANG Ningbo | 2013 | 中国电机工程学报2013，33，11： | 1 |
| 19 | Role of Endogenous Glucagon-Like Peptide-1 in Islet Regeneration After Partial Pancreatectomy显示文摘 | Diva D De Leon Shaoping Deng Reza Madani | 2003 | Diabetes2003，52，： | 1 |
| 20 | Identification of benzofuran central cores for the inhibition of leukotriene A 4 hydrolase显示文摘 | Wendy Eccles Jonathan M. Blevitt Jamila N. Booker Christa C. Chrovian Shelby Crawford Aimee Rose de Leon Xiaohu Deng Anne M. Fourie Cheryl A. Grice Krystal Herman Lars Karlsson Aaron M. Kearney Alice Lee-Dutra Jimmy Liang Rosa Luna Dan Pippel Navin Rao Ja | 2012 | Bioorganic & Medicinal Chemistry Letters2012，，： | 1 |
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