|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Object-based classification approach for greenhouse mapping using Landsat-8 imagery显示文摘Suburban greenhouses with intensive agricultural productivity have increasingly influenced the daily diet and vegetable supply in Chinese cities.With their enormous input of fertilizers and pesticides,greenhouses have considerably changed the local soil quality and environmental risk factors.The ability to obtain timely and accurate information regarding the spatial distribution of greenhouses could make an important contribution to local agricultural management and soil protection.This paper attempts to present a practical framework for extracting suburban greenhouses,integrating remote sensing data from Landsat-8 and object-oriented classification.Inheritance classification was implemented,and various properties,including texture and neighborhood features in addition to spectral information,were investigated through the popular random forest technique for feature selection prior to SVM classification to improve the mapping accuracy.The results demonstrated that object-based classification incorporating non-spectral features yielded a significant improvement compared with the classification results obtained using only the spectral information in traditional per-pixel classification.Both the producer’s and user’s accuracy were higher than 85%for greenhouse identification.Although it remained a challenge to completely distinguish greenhouses from sparse plants,the final greenhouse map indicated that the proposed object-based classification scheme,providing multiple feature selections and multi-scale analysis,yielded worthwhile information when applied to a continuous series of the freely available Landsat-8 imagery data. | Wu Chaofan Deng Jinsong Wang Ke Ma Ligang Amir Reza Shah Tahmassebi | 2016 | International Journal of Agricultural and Biological Engineering2016，9，1： | 7 |
| 2 | A draft sequence of the rice (Oryza sativa ssp. indica) genome显示文摘The sequence of the rice genome holds fundamental information for its biology, including physiology, genetics, development, and evolution, as well as information on many beneficial phenotypes of economic significance. Using a 'whole genome shotgun' approach, we have pro-duced a draft rice genome sequence of Oryza sativa ssp. in-dica, the major crop rice subspecies in China and many other regions of Asia. The draft genome sequence is constructed from over 4.3 million successful sequencing traces with an accumulative total length of 2214.9 Mb. The initial assembly of the non-redundant sequences reached 409.76 Mb in length, based on 3.30 million successful sequencing traces with a total length of 1797.4 Mb from an indica variant cultivar 93-11, giving an estimated coverage of 95.29% of the rice genome with an average base accuracy of higher than 99%. The coverage of the draft sequence, the randomness of the sequence distribution, and the consistency of BIG-ASSEM-BLER, a custom-designed software package | YU Jun, HU Songnian, WANG Jun,LI Songgang WONG Ka-Shu Gane, LIU Bin,DENG Yajun, DAI Li, ZHOU Yan,ZHANG Xiuqing, CAO Mengliang, LIU Jing,SUN Jiandong , TANG Jiabin, CHEN Yanjiong,HUANG Xiaobing, LIN Wei, YE Chen, TONG Wei,CONG Lijuan, GENG Jianing, HAN Yujun, LI Lin,LI Wei, HU Guangqiang, HUANG Xiangang,LI Wenjie, LI Jian, LIU Zhanwei, LI Long,LIU Jianping, Ql Qiuhui, LIU Jinsong, LI Li,WANG Xuegang, LU Hong, WU Tingling,ZHU Miao, Nl Peixiang, HAN Hua, DONG Wei,REN Xiaoyu, FENG Xiaoli, GUI Peng,LI Xianran, WANG Hao, XU Xin, ZHAI Wenxue,XU Zhao, ZHANG Jinsong, HE Sijie,ZHANG Jianguo, XU Jichen, ZHANG Kunlin,ZHENG Xianwu, DONG Jianhai, ZENG Wanyong,TAO Lin, CHEN Xuewei, HE Jun, LIU Daofeng,TIAN Wei, TIAN Chaoguang, XIA Hongai,LI Gang, GAO Hui, LI Ping, CHEN Wei ,WANG Xudong, ZHANG Yong, HU Jianfei,WANG Jing, LIU Song, YANG Jian,ZHANG Guangyu, XIONG Yuqing, LI Zhijie,MAO Long, ZHOU Chengshu, ZHU Zhen,CHEN Runsheng, HAO Bailin,ZHENG Weimou, CHEN Shouyi, QUO Wei,LI Guojie, LIU Siqi, HUANG Guyang,TAO Ming, WANG Jian, ZHU Lihuang,YUAN Longping& YANG HuanmingBeijing Genomics Institute/Center of Genomics & Bioinformatics, Chinese Academy of Sciences, Beijing 101300, China Hangzhou Genomics Institute/Institute of Bioinformatics of Zhejiang University/Key Laboratory of Bioinformatics of Zhejiang Province, Hangzhou 310007, China Institute of Genetics, Chinese Academy of Sciences, Beijing 100101, China National Hybrid Rice R & D Center, Changsha 410125, China Laboratory of Bioinformatics, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China College of Life Sciences, Peking University, Beijing 100871, China Institute of Theoretical Physics, Chinese Academy of Sciences, Beijing 1Q0080, China Digital China Ltd., Beijing 100080, China Institute of Computing Technology, Chinese Academy of Sciences, Beijing 100080, China Medical College, Xi’an Jiaotong University, Xi’an 710061, ChinaThese authors contributed equally to this work.Corresponding author.Corresponden | 2001 | Chinese Science Bulletin2001，46，23： | 6 |
| 3 | Fiber Bragg grating monitors for thermal and stress of the composite insulators in transmission lines显示文摘Running composite insulators are prone to failure due to their harsh surrounding work environment, which directly affects the safe operation of transmission lines. This paper puts forward the method of using fiber Bragg grating(FBG) as the monitors to parameters correlated with thermal and stress of the composite insulators in transmission lines at working status. Firstly, monitoring points are found out by the mechanical test on composite insulator samples. Secondly, based on the monitoring theory, this paper introduces the feasibility design frame of the composite insulator with FBG implanted in the rod and the online monitor system. At last, it describes applications of this monitor system in the field of transmission lines. | Heming Deng Wei Cai You Song Jinsong Liu Christopher Redman Qiandong Zhuang | 2018 | Global Energy Interconnection2018，1，3： | 6 |
| 4 | Multi-foci metalens for spectra and polarization ellipticity recognition and reconstruction显示文摘Multispectral and polarized focusing and imaging are key functions that are vitally important for a broad range of optical applications.Conventional techniques generally require multiple shots to unveil desired optical information and are implemented via bulky multi-pass systems or mechanically moving parts that are difficult to integrate into compact and integrated optical systems.Here,a design of ultra-compact transversely dispersive metalens capable of both spectrum and polarization ellipticity recognition and reconstruction in just a single shot is demonstrated with both coherent and incoherent light.Our design is well suited for integrated and high-speed optical information analysis and can significantly reduce the size and weight of conventional devices while simplifying the process of collecting optical information,thereby promising for various applications,including machine vision,minimized spectrometers,material characterization,remote sensing,and other areas which require comprehensive optical analysis. | Hui Gao Xuhao Fan Yuxi Wang Yuncheng Liu Xinger Wang Ke Xu Leimin Deng Cheng Zeng Tingan Li Jinsong Xia Wei Xiong | 2023 | Opto-Electronic Science2023，2，3： | 3 |
| 5 | Colonization of endophyte Acremonium sp. D212 in Panax notoginseng and rice mediated by auxin and jasmonic acid显示文摘Endophytic fungi can be beneficial to plant growth. However, the molecular mechanisms under-lying colonization of Acremonium spp. remain unclear. In this study, a novel endophytic Acremonium strain was isolated from the buds of Panax notoginseng and named Acremonium sp. D212. The Acremonium sp. D212 could colonize the roots of P. notoginseng, enhance the resistance of P. notoginseng to root rot disease, and promote root growth and saponin bio-synthesis in P. notoginseng. Acremonium sp. D212 could secrete indole-3-acetic acid (IAA) and jasmonic acid (JA), and inoculation with the fungus increased the endogenous levels of IAA and JA in P. noto-ginseng. Colonization of the Acremonium sp. D212 in the roots of the rice line Nipponbare was dependent on the concentration of methyl jasmonate (MeJA) (2–15μmol/L) and 1-naphthalenacetic acid (NAA) (10–20μmol/L). Moreover, the roots of the JA signaling-defective coi1-18 mutant were colonized by Acremonium sp. D212 to a lesser degree than those of the wild-type Nipponbare and miR393b-overexpressing lines, and the colonization was res-cued by MeJA but not by NAA. It suggests that the cross-talk between JA signaling and the auxin biosynthetic pathway plays a crucial role in the colonization of Acremonium sp. D212 in host plants. | Li Han Xuan Zhou Yiting Zhao Shusheng Zhu Lixia Wu Yunlu He Xiangrui Ping Xinqi Lu Wuying Huang Jie Qian Lina Zhang Xi Jiang Dan Zhu Chongyu Luo Saijie Li Qian Dong Qijing Fu Kaiyuan Deng Xin Wang Lei Wang Sheng Peng Jinsong Wu Weimin Li JiǐíFriml Youyong Zhu Xiahong He Yunlong Du | 2020 | Journal of Integrative Plant Biology2020，62，9： | 2 |
| 6 | Eco-environmental vulnerability assessment for large drinking water resource: a case study of Qiandao Lake Area, China显示文摘卡恩道·莱克区域(QLA ) 以在东方海岸中国以及一个全国地著名的旅游胜地喝水供应意义大。关于 QLA 环境的一系列法律和规定被制定了并且全部与有害效果与扩展都市化和工业化联系了的否定的目的实现了过去的十年。在这研究,一个评价框架被开发由在一次尝试集成模糊分析层次过程( FAHP )和地理信息系统( GIS )获得卓见进 QLA 的现状以便考察并且评估相关政策的有效性从 19902010 分析 QLA 的eco环境的危险。在在 QLA 处理并且分析 eco 环境的危险和主要环境问题的时间、空间的变化以后,我们发现 QLA 的 eco 环境的危险的国家是可接受的,尽管中等恶化在学习时期期间被检测。而且,危险和水质量的联合的分析显示水质量显示出偏斜的符号,尽管全面地位仍然保持令人满意。集体保护和治疗行动在学习经期上是有效的,这因此被结束,而立即更严格的措施将为保护喝的水质量免受国内污水和工业废水的伤害被要求。最后, eco 环境的危险评价的空间变化也暗示明确地,更多的指向的措施应该为 QLA 的长期的持续开发在各自的区域被采用。 | Qing GU Jun LI Jinsong DENG Yi LIN LigangMA Chaofan WU Ke WANG Yang HONG | 2015 | Frontiers of Earth Science2015，9，3： | 2 |
| 7 | High proportion strontium-doped micro-arc oxidation coatings enhance early osseointegration of titanium in osteoporosis by anti-oxidative stress pathway显示文摘The excessive accumulation of reactive oxygen species(ROS)under osteoporosis precipitates a microenvironment with high levels of oxidative stress(OS).This could significantly interfere with the bioactivity of conventional titanium implants,impeding their early osseointegration with bone.We have prepared a series of strontium(Sr)-doped titanium implants via micro-arc oxidation(MAO)to verify their efficacy and differences in osteoinduction capabilities under normal and osteoporotic(high OS levels)conditions.Apart from the chemical composition,all groups exhibited similar physicochemical properties(morphology,roughness,crystal structure,and wettability).Among the groups,the low Sr group(Sr25%)was more conducive to osteogenesis under normal conditions.In contrast,by increasing the catalase(CAT)/superoxide dismutase(SOD)activity and decreasing ROS levels,the high Sr-doped samples(Sr75% and Sr100%)were superior to Sr25% in inducing osteogenic differentiation of MC3T3-E1 cells and the M2 phenotype polarization of RAW264.7 cells,thus enhancing early osseointegration.Furthermore,the results of both in vitro cell co-culture and in vivo studies also showed that the high Sr-doped samples(especially Sr100%)had positive effects on osteoimmunomodulation under the OS microenvironment.Ultimately,the collated findings indicated that the high proportion Sr-doped MAO coatings were more favorable for osteoporosis patients in implant restorations. | Xinkun Shen Kai Fang Kendrick Hii Ru Yie Zixin Zhou Yiding Shen Shuyi Wu Yue Zhu Zhennan Deng Pingping Ma Jianfeng Ma Jinsong Liu | 2022 | Bioactive Materials2022，7，4： | 2 |
| 8 | The Solar Upper Transition Region Imager(SUTRI)Onboard the SATech-01 Satellite显示文摘The Solar Upper Transition Region Imager(SUTRI)onboard the Space Advanced Technology demonstration satellite(SATech-01),which was launched to a Sun-synchronous orbit at a height of~500 km in 2022 July,aims to test the on-orbit performance of our newly developed Sc/Si multi-layer reflecting mirror and the 2k×2k EUV CMOS imaging camera and to take full-disk solar images at the Ne VII 46.5 nm spectral line with a filter width of~3 nm.SUTRI employs a Ritchey-Chrétien optical system with an aperture of 18 cm.The on-orbit observations show that SUTRI images have a field of view of~416×416 and a moderate spatial resolution of~8″without an image stabilization system.The normal cadence of SUTRI images is 30 s and the solar observation time is about16 hr each day because the earth eclipse time accounts for about 1/3 of SATech-01's orbit period.Approximately15 GB data is acquired each day and made available online after processing.SUTRI images are valuable as the Ne VII 46.5 nm line is formed at a temperature regime of~0.5 MK in the solar atmosphere,which has rarely been sampled by existing solar imagers.SUTRI observations will establish connections between structures in the lower solar atmosphere and corona,and advance our understanding of various types of solar activity such as flares,filament eruptions,coronal jets and coronal mass ejections. | Xianyong Bai Hui Tian Yuanyong Deng Zhanshan Wang Jianfeng Yang Xiaofeng Zhang Yonghe Zhang Runze Qi Nange Wang Yang Gao Jun Yu Chunling He Zhengxiang Shen Lun Shen Song Guo Zhenyong Hou Kaifan Ji Xingzi Bi Wei Duan Xiao Yang Jiaben Lin Ziyao Hu Qian Song Zihao Yang Yajie Chen Weidong Qiao Wei Ge Fu Li Lei Jin Jiawei He Xiaobo Chen Xiaocheng Zhu Junwang He Qi Shi Liu Liu Jinsong Li Dongxiao Xu Rui Liu Taijie Li Zhenggong Feng Yamin Wang Chengcheng Fan Shuo Liu Sifan Guo Zheng Sun Yuchuan Wu Haiyu Li Qi Yang Yuyang Ye Weichen Gu Jiali Wu Zhe Zhang Yue Yu Zeyi Ye Pengfeng Sheng Yifan Wang Wenbin Li Qiushi Huang Zhong Zhang | 2023 | Research in Astronomy and Astrophysics2023，23，6： | 1 |
| 9 | Study on the enhanced bio-denitrification in a full-scale WWTP with MSBR process 显示文摘 | DENG Renjian ZHANG Jinsong Qu Zhijun | | Advanced Materials Research0，610，1： | 1 |
| 10 | Energy and Parameter Analysis of SOFC System for Hydrogen Production from Methane Steam Reforming显示文摘In this paper, an integrated system of solid oxide fuel cell(SOFC) and methane steam reforming for hydrogen production is proposed. The mathematical model of the coupled integrated system is studied by COMSOL and Aspen software, and the energy analysis of the integrated system is carried out. The system recovers and reuses the waste heat of the SOFC stack through the heat exchanger, which can realize the cascade efficient use of energy. By adjusting the different reforming temperatures, steam-to-carbon ratio and SOFC operating temperature of methane steam reforming to produce hydrogen, the parameters that have a greater impact on the system are studied. The research results show that as the steam-to-carbon ratio and reformer operating temperature increase, the net output power and efficiency of the system increase. When the fuel cell operating temperature is 800℃, the output power and efficiency of the system reach the maximum values of 899.93 W and 52.52%, respectively. Increasing the operating temperature of SOFC helps to improve the efficiency of fuel cells, but the efficiency of the integrated system of methane steam reforming hydrogen production and SOFC first increases and then decreases. This system can realize the direct coupling between the SOFC reactor subsystem and the methane steam reforming hydrogen production system under optimized conditions, which has reference significance for the actual operating conditions of the coupled system. | DENG Meilong LIU Jinyi ZHANG Xiaosong LI Jinsong FU Lirong | 2022 | Journal of Thermal Science2022，31，6： | 1 |
| 11 | Decomposition of two haloacetic acids in water using UV radiation, ozone and advanced oxidation processes显示文摘 | Kunping Wang Jinsong Guo Min Yang Hirotsuji Junji Rongsen Deng | 2008 | Journal of Hazardous Materials2008，，2： | 1 |
| 12 | Specific generation of nitric oxide in mitochondria of cancer cell for selective oncotherapy显示文摘Nitric oxide(NO)gas therapy,especially,L-arginine(L-Arg)-based NO treatment strategies have attracted extensive attention in the field of oncotherapy.However,current strategies are unable to differentiate well between normal cells and cancer cells,which may lead to unpredictable toxicity.Motivated by the fact that mitochondria of cancer cells can express excessive nitric oxide synthetase(NOS),herein,a nanozyme-based NO generator,cerium oxide(CeO_(2))-AT,is fabricated to specifically catalyze the production of NO in cancer cells for selective tumor treatment.In this system,after being endocytosed into cancer cells,the generator can produce a number of NO under the catalysis of NOS in mitochondria of cancer cells,which can disrupt the mitochondrial respiratory chain of tumor cells and further induce cell apoptosis.In addition,the generator with catalase(CAT)-like activity can catalyze H_(2)O_(2)to produce O_(2),which can promote the generation of NO and improve the performance of NO gas therapy.What is more,our system has no obvious impact on the viability of normal cells owing to the less production of NO.Our work paves a new way for the development of highly selective NO-based treatment particularly useful for the safe and specific cancer therapy. | Mengyu Sun Yanjuan Sang Qingqing Deng Zhengwei Liu Jinsong Ren Xiaogang Qu | 2022 | Nano Research2022，15，6： | 1 |
| 13 | An inte- grated analysis of urbanization-triggered cropland loss trajectory and implications for sustainable land manage- ment 显示文摘 | Deng Jinsong Qiu Lefeng Wang Ke | 2011 | Cities2011，28，2： | 1 |
| 14 | Meniscus fabrication of halide perovskite thin films at high throughput for large area and low-cost solar panels显示文摘Halide perovskites have rapidly attracted considerable attention due to unprecedented properties not seen in traditional semiconductors.In addition to their optoelectronic merits,one advantage of perovskite materials is their solution processability,which opens the door to low-cost and high throughput solution coating strategies for the commercialization of perovskite solar cells(PSCs).Here we review perovskite film fabrication by meniscus coating—a simple and readily scalable manufacturing technique,including blade coating and slot-die coating.We outline the fundamental fluid mechanisms of meniscus coating,discuss drying and crystallization of perovskite in the coating process,and provide an overview of recent progress in meniscus-coated PSCs. | Xuezeng Dai Yehao Deng Charles H Van Brackle Jinsong Huang | 2019 | International Journal of Extreme Manufacturing2019，1，2： | 1 |
| 15 | Elucidating dominant factors of PO43–, Cd2+ and nitrobenzene removal by biochar: A comparative investigation based on distinguishable biochars显示文摘Biochars produced from crab shell(CSB),oak sawdust(OB),Jeru salem artichoke tuber(JAB) and sorghum grain(SB) displayed distinguishable adsorption-related characteristics,such as specific surface area(SSA),ash content and acidic oxygen-containing functional groups(AFGs),which linked to the biochar adsorption mechanisms of most pollutants.Herein,PO4^3-,Cd^2+,and nitrobenzene(NB) were employed for adsorption by these biochars to elucidate the dominant factors for the adsorption.Adsorption performance of the three pollutants onto these four biochars varied considerably,as exemplified by the excellent adsorption of PO4^3- and Cd^2+ onto CSB(225.3 and 116.0 mg/g,respectively) as compared with onto the other three biochars(4.2-37.1 mg/g for P043-and 9.7-41.0 mg/g for Cd^2+).OB displayed the best adsorption of NB(72.0 mg/g),followed by SB(39.5 mg/g),JAB(31.1 mg/g),and CSB(23.6 mg/g).The kinetics and isotherm adsorption assessments couple with material characterization suggested that the sorption of selected pollutants on biochars was attributed to the multiple mechanisms involved,including coprecipitation,chemical bonds,cation exchange,physical absorption,and complexation.Further path analysis suggested that AFGs and ash content in biochars were more important than SSA with regards to pollutant removal,especially,with ash playing a crucial role in the removal of Cd2+ and PO43-,and AFGs being mainly re s ponsible for NB adsorption.These findings might offer guidance on the preparation or modification of biochar with a targeted function for pollutant removal through an understanding the dominant factors. | Zhanglin Liu Dong Tian Fei Shen Lulu Long Yanzong Zhang Gang Yang Yongmei Zeng Jing Zhang Jinsong He Ying Zhu Shihuai Deng | 2019 | Chinese Chemical Letters2019，30，12： | 0 |
| 16 | Log-cumulants of the finite mixture model and their application to statistical analysis of fully polarimetric UAVSAR data显示文摘Since its first flight in 2007,the UAVSAR instrument of NASA has acquired a large number of fully Polarimetric SAR(PolSAR)data in very high spatial resolution.It is possible to observe small spatial features in this type of data,offering the opportunity to explore structures in the images.In general,the structured scenes would present multimodal or spiky histograms.The finite mixture model has great advantages in modeling data with irregular histograms.In this paper,a type of important statistics called log-cumulants,which could be used to design parameter estimator or goodness-of-fit tests,are derived for the finite mixture model.They are compared with logcumulants of the texture models.The results are adopted to UAVSAR data analysis to determine which model is better for different land types. | Xinping Deng Jinsong Chen Hongzhong Li Pengpeng Han Wen Yang | 2018 | Geo-Spatial Information Science2018，21，1： | 0 |
| 17 | Chemical weathering profile in the V–Ti–Fe mine tailings pond:a basalt-weathering analog显示文摘The(ultra-)mafic mine tailings pond revealed a weathering discrepancy in the tailings profile,which provided a valuable analog to assess the role of carbonate and silicate weathering of the basalt.In this study,drill-cores samples were selected from the Wanniangou V–Ti–Fe mine tailings pond(Sichuan province,China)to investigate the mineralogicand geochemical characteristics in the tailings profile.The results reveal(1)the tailings pond profile consist of upper and lower layers.The upper layer is composed of carbonate weathering(1.4%),which was formed in the initial stages of tailings exposure and represented a minimal weathering degree.(2)The lower layer was primarily observed at the aquifer zone of the tailings pond,and was consistent with 0.45%carbonate weathering and 48.4%silicate weathering.(3)The weathering discrepancy in the tailings profile could be due to the sulfide oxidation and aerobic/flowing aquifer,which facilitate the water-tailings reactions.The tailings profile provides an analog to studying basalt weathering,as it spans both carbonate and silicate weathering.This research reinforces the idea that silicate weathering is predominant in basaltic areas and plays a crucial role in regulating atmospheric CO_(2)(carbon dioxide)levels on Earth. | Xiaolin Zhang Yinger Deng Liang Tang Zhengmeng Hou Jinsong Yang | 2023 | Acta Geochimica2023，42，6： | 0 |
| 18 | Space advanced technology demonstration satellite显示文摘The Space Advanced Technology demonstration satellite(SATech-01),a mission for low-cost space science and new technology experiments,organized by Chinese Academy of Sciences(CAS),was successfully launched into a Sun-synchronous orbit at an altitude of~500 km on July 27,2022,from the Jiuquan Satellite Launch Centre.Serving as an experimental platform for space science exploration and the demonstration of advanced common technologies in orbit,SATech-01 is equipped with 16 experimental payloads,including the solar upper transition region imager(SUTRI),the lobster eye imager for astronomy(LEIA),the high energy burst searcher(HEBS),and a High Precision Magnetic Field Measurement System based on a CPT Magnetometer(CPT).It also incorporates an imager with freeform optics,an integrated thermal imaging sensor,and a multi-functional integrated imager,etc.This paper provides an overview of SATech-01,including a technical description of the satellite and its scientific payloads,along with their on-orbit performance. | ZHANG XiaoFeng CHEN Wen ZHU XiaoCheng MENG Na HE JunWang BI XingZi ZHANG YongHe SHI Qi LI Fei LIU Rui FENG ZhengGong LIU Liu LI JinSong WU HaiChen XU DongXiao LI TaiJie HUANG JiangJiang LIU Shuo LI TianTong YU XianSheng GAO Yang ZHOU Heng BAN HanYu ZHANG YanLi ZHANG YueTing YANG YingQuan HE Tao DUAN XuLiang CHEN Xin WANG YaMin SUN AnTai ZHANG KuoXiang SUN Ying WANG YaoBin FAN ChengCheng XIONG ShaoLin LI XinQiao WEN XiangYang LING ZhiXing SUN XiaoJin ZHANG Chen BAI XianYong WANG ZhanShan DENG YuanYong TIAN Hui YANG JianFeng XUE HongBo SANG Peng LIU JinGuo ZHENG HuiLong ZHU Xiang HE JianWu LI Hui XU LuXiang XU ShuYan CHEN WenWu LIU ZhenDong WANG ZhaoLi MAO XiangLong GAO Rong LI ZongXuan DING GuoPeng WANG XinYu DOU RunJiang WENG LuBin LUO Hao WANG YaPing LIANG XianFeng FANG ZiRuo | 2024 | Science China(Technological Sciences)2024，67，1： | 0 |
| 19 | RNA structure determination:From 2D to 3D显示文摘RNA molecules serve a wide range of functions that are closely linked to their structures.The basic structural units of RNA consist of single-and double-stranded regions.In order to carry out advanced functions such as catalysis and ligand binding,certain types of RNAs can adopt higher-order structures.The analysis of RNA structures has progressed alongside advancements in structural biology techniques,but it comes with its own set of challenges and corresponding solutions.In this review,we will discuss recent advances in RNA structure analysis techniques,including structural probing methods,X-ray crystallography,nuclear magnetic resonance,cryo-electron microscopy,and small-angle X-ray scattering.Often,a combination of multiple techniques is employed for the integrated analysis of RNA structures.We also survey important RNA structures that have been recently determined using various techniques. | Jie Deng Xianyang Fang Lin Huang Shanshan Li Lilei Xu Keqiong Ye Jinsong Zhang Kaiming Zhang Qiangfeng Cliff Zhang | 2023 | Fundamental Research2023，3，5： | 0 |
| 20 | NR5A2 connects zygotic genome activation to the first lineage segregation in totipotent embryos显示文摘Zygotic genome activation(ZGA)marks the beginning of the embryonic program for a totipotent embryo,which gives rise to the inner cell mass(ICM)where pluripotent epiblast arises,and extraembryonic trophectoderm.However,how ZGA is connected to the first lineage segregation in mammalian embryos remains elusive.Here,we investigated the role of nuclear receptor(NR)transcription factors(TFs),whose motifs are highly enriched and accessible from the 2-cell(2C)to 8-cell(8C)stages in mouse embryos.We found that NR5A2,an NR TF strongly induced upon ZGA,was required for this connection.Upon Nr5a2 knockdown or knockout,embryos developed beyond 2C normally with the zygotic genome largely activated.However,4–8C-specific gene activation was substantially impaired and Nr5a2-deficient embryos subsequently arrested at the morula stage.Genome-wide chromatin binding analysis showed that NR5A2-bound cis-regulatory elements in both 2C and 8C embryos are strongly enriched for B1 elements where its binding motif is embedded.NR5A2 was not required for the global opening of its binding sites in 2C embryos but was essential to the opening of its 8C-specific binding sites.These 8C-specific,but not 2C-specific,binding sites are enriched near genes involved in blastocyst and stem cell regulation,and are often bound by master pluripotency TFs in blastocysts and embryonic stem cells(ESCs).Importantly,NR5A2 regulated key pluripotency genes Nanog and Pou5f1/Oct4,and primitive endoderm regulatory genes including Gata6 among many early ICM genes,as well as key trophectoderm regulatory genes including Tead4 and Gata3 at the 8C stage.By contrast,master pluripotency TFs NANOG,SOX2,and OCT4 targeted both early and late ICM genes in mouse ESCs.Taken together,these data identify NR5A2 as a key regulator in totipotent embryos that bridges ZGA to the first lineage segregation during mouse early development. | Fangnong Lai Lijia Li Xiaoyu Hu Bofeng Liu Ziqi Zhu Ling Liu Qiang Fan Huabin Tian Kai Xu Xukun Lu Qing Li Kong Feng Lijuan Wang Zili Lin Hongyu Deng Jinsong Li Wei Xie | 2023 | Cell Research2023，33，12： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员