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| 1 | Trends in cancer mortality in China from 2004 to 2018:A nationwide longitudinal study显示文摘Background:The long-term trend in cancer death in a rapidly developing country provides information for cancer prophylaxis.Here,we aimed to identify the trends in cancer mortality in China during the 2004-2018 period.Methods:Using raw data from the national mortality surveillance system of China,we assessed the mortalities of all cancer and site-specific cancers during the 2004-2018 period.The participants were divided into three age groups:≥65 years,40-64 years,and≤39 years.Changing trends in cancer death by gender,residency,and tumor location were estimated using fitting joinpoint models to log-transformed crude mortality rates(CMRs)and age-standardizedmortality rates(ASMRs).Results:Cancer death accounted for 24% of all-cause of death in China during 2014-2018.The CMR of all cancer was 150.0 per 100,000 persons.Cancer was the leading cause of death in the population<65 years.The six major cancer types(lung/bronchus cancer,liver cancer,stomach cancer,esophagus cancer,colorectal cancer,and pancreas cancer)accounted for 75.85% of all cancer deaths.The CMR of all cancer increased while the ASMR decreased during 2014-2018(P<0.001).Lung/bronchus cancer and liver cancer were the leading causes of cancer death in the population<65 years,accounting for 45.31%(CMR)and 44.35%(ASMR)of all cancer death,respectively.The ASMR of liver cancer was higher in the 40-64 years population than in the≥65 years population,in contrast to the other five major cancers.The ASMRs of liver cancer,stomach cancer,and esophagus cancer decreased although they were higher in rural residents than in urban residents;the ASMRs of lung/bronchus cancer,colorectal cancer,and pancreas cancer increased in rural residents although they were higher in urban residents than in rural residents during 2014-2018.Conclusion:Although the ASMR of all cancer decreased in China during 2004-2018,lung/bronchus cancer and liver cancer remained the leading causes of cancer-related premature death.Lung/bronchus cancer,colorectal cancer,and pancreas cancer increased in rural residents. | Dongming Jiang Lijuan Zhang Wenbin Liu Yibo Ding Jianhua Yin Rongbing Ren Qi Li Yifan Chen Jiaying Shen Xiaojie Tan Hongwei Zhang Guangwen Cao | 2021 | Cancer Communications2021，41，10： | 23 |
| 2 | Review of control and guidance technology on hypersonic vehicle显示文摘Air-breathing hypersonic vehicle has great military and potential economic value due to its characteristics:high velocity,long range,quick response.Therefore,the development of hypersonic vehicle and its guidance and control technology are reviewed in this paper.Firstly,the development and classification of hypersonic vehicles around the world are summarized,and the geometric configuration and mission profile of typical air-breathing hypersonic vehicle are given.Secondly,the control difficulties of air-breathing hypersonic vehicle are introduced,including integrated design of engine and fuselage,static instability,strong nonlinearity,uncertain aerodynamic parameters,etc.According to its control requirements,the control methods considering external disturbance,fault-tolerant control methods,anti-saturation methods,and prescribed performance control methods considering transient performance constraints are summarized respectively.The classification and comparison of various control methods are given,and the frontiers of theoretical development are analyzed.Finally,considering the effects of composite disturbances,the design of terminal guidance law under multiple constraints is overviewed,including guidance law with angle constraint,velocity constraint,acceleration constraint and time constraint.Similarly,the classification of guidance law design methods under different constraints,their advantages as well as the future development trend and requirements are introduced. | Yibo DING Xiaokui YUE Guangshan CHEN Jiashun SI | 2022 | Chinese Journal of Aeronautics2022，35，7： | 9 |
| 3 | Diet drives convergent evolution of gut microbiomes in bamboo-eating species显示文摘Gut microbiota plays a critical role in host physiology and health.The coevolution between the host and its gut microbes facilitates animal adaptation to its specific ecological niche.Multiple factors such as host diet and phylogeny modulate the structure and function of gut microbiota.However,the relative contribution of each factor in shaping the structure of gut microbiota remains unclear.The giant(Ailuropoda melanoleuca)and red(Ailurus styani)pandas belong to different families of order Carnivora.They have evolved as obligate bamboo-feeders and can be used as a model system for studying the gut microbiome convergent evolution.Here,we compare the structure and function of gut microbiota of the two pandas with their carnivorous relatives using 16S rRNA and metagenome sequencing.We found that both panda species share more similarities in their gut microbiota structure with each other than each species shares with its carnivorous relatives.This indicates that the specialized herbivorous diet rather than host phylogeny is the dominant driver of gut microbiome convergence within Arctoidea.Metagenomic analysis revealed that the symbiotic gut microbiota of both pandas possesses a high level of starch and sucrose metabolism and vitamin B12 biosynthesis.These findings suggest a diet-driven convergence of gut microbiomes and provide new insight into host-microbiota coevolution of these endangered species. | Guangping Huang Xiao Wang Yibo Hu Qi Wu Yonggang Nie Jiuhong Dong Yun Ding Li Yan Fuwen Wei | 2021 | Science China(Life Sciences)2021，64，1： | 6 |
| 4 | The RppC-AvrRppC NLR-effector interaction mediates the resistance to southern corn rust in maize显示文摘Southern corn rust(SCR),caused by the fungal pathogen Puccinia polysora,is a major threat to maize pro-duction worldwide.Efficient breeding and deployment of resistant hybrids are key to achieving durable control of SCR.Here,we report the molecular cloning and characterization of RppC,which encodes an NLR-type immune receptor and is responsible for a major SCR resistance quantitative trait locus.Further-more,we identified the corresponding avirulence effector,AvrRppC,which is secreted by P.polysora and triggers RppC-mediated resistance.Allelic variation of AvrRppC directly determines the effectiveness of RppC-mediated resistance,indicating that monitoring of AvrRppC variants in the field can guide the rational deployment of RppC-containing hybrids in maize production.Currently,RppC is the most frequently deployed SCR resistance gene in China,and a better understanding of its mode of action is crit-ical for extending its durability. | Ce Deng April Leonard James Cahill Meng Lv Yurong Li Shawn Thatcher Xueying Li Xiaodi Zhao Wenjie Du Zheng Li Huimin Li Victor Llaca Kevin Fengler Lisa Marshall Charlotte Harris Girma Tabor Zhimin Li Zhiqiang Tian Qinghua Yang Yanhui Chen Jihua Tang Xintao Wang Junjie Hao Jianbing Yan Zhibing Lai Xiaohong Fei Weibin Song Jinsheng Lai Xuecai Zhang Guoping Shu Yibo Wang Yuxiao Chang Weiling Zhu Wei Xiong Juan Sun Bailin Li Junqiang Ding | 2022 | Molecular Plant2022，15，5： | 6 |
| 5 | Follistatin-like protein 1 plays a tumor suppressor role in clear-cell renal cell carcinoma显示文摘Background:We previously showed that the expression of follistatin-like protein 1(FSTL1)was significantly down-regulated in metastatic clear-cell renal cell carcinoma(ccRCC).In this study,we aimed to characterize the role of FSTL1 in the development of ccRCC.Methods:The effects of FSTL1 on cell activity and cell cycle were investigated in ccRCC cell lines with altered FSTL1 expression.Gene expression microarray assays were performed to identify the major signaling pathways affected by FSTL1 knockdown.The expression of FSTL1 in ccRCC and its effect on postoperative prognosis were estimated in a cohort with 89 patients.Results:FSTL1 knockdown promoted anchorage-independent growth,migration,invasion,and cell cycle of ccRCC cell lines,whereas FSTL1 overexpression attenuated cell migration.FSTL1 knockdown up-regulated nuclear factor-κB(NF-κB)and hypoxia-inducible factor(HIF)signaling pathways,increased epithelial-to-mesenchymal transition,up-regulated interleukin-6 expression,and promoted tumor necrosis factor-α-induced degradation of NF-κB inhibitor(IκBα)in ccRCC cell lines.FSTL1 immunostaining was selectively positive in epithelial cytoplasm in the loop of Henle,and positive rate of FSTL1 was significantly lower in ccRCC tissues than in adjacent renal tissues(P<0.001).The mul-tivariate Cox regression analysis showed that the intratumoral FSTL1 expression conferred a favorable independent prognosis with a hazard ratio of 0.325(95%confidence interval 0.118-0.894).HIF-2αexpression was negatively cor-related with FSTL1 expression in ccRCC specimens(r=−0.229,P=0.044).Intratumoral expression of HIF-2α,rather than HIF-1α,significantly predicted an unfavorable prognosis in ccRCC(log-rank,P=0.038).Conclusions:FSTL1 plays a tumor suppression role possibly via repressing the NF-κB and HIF-2αsignaling pathways.To increase FSTL1 expression might be a candidate therapeutic strategy for metastatic ccRCC. | Yan Liu Xiaojie Tan Wenbin Liu Xi Chen Xiaomei Hou Dan Shen Yibo Ding Jianhua Yin Ling Wang Hongwei Zhang Yongwei Yu Jianguo Hou Timothy C.Thompson Guangwen Cao | 2018 | Cancer Communications2018，38，1： | 5 |
| 6 | The pulsed high magnetic field facility and scientific research at Wuhan National High Magnetic Field Center显示文摘Wuhan National High Magnetic Field Center(WHMFC)at Huazhong University of Science and Technology is one of the top-class research centers in the world,which can offer pulsed fields up to 90.6 T with different field waveforms for scientific research and has passed the final evaluation of the Chinese government in 2014.This paper will give a brief introduction of the facility and the development status of pulsed magnetic fields research at WHMFC.In addition,it will describe the application development of pulsed magnetic fields in both scientific and industrial research. | Xiaotao Han Tao Peng Hongfa Ding Tonghai Ding Zengwei Zhu Zhengcai Xia Junfeng Wang Junbo Han Zhongwen Ouyang Zhenxing Wang Yibo Han Houxiu Xiao Quanliang Cao Yiliang Lv Yuan Pan Liang Li | 2017 | Matter and Radiation at Extremes2017，2，6： | 5 |
| 7 | Proteomic profiling of lipid droplet-associated proteins in primary adipocytes of normal and obese mouse显示文摘在 adipocytes 的类脂化合物微滴用作动物的主要长期的精力存储仓库。正在增加类脂化合物微滴不仅仅是一个静态的中立类脂化合物存储地点,而是事实上有活力、多功能的细胞器的识别。在结构上,类脂化合物微滴由嵌入在或跳了到 phospholipid 层的 phospholipid 单层和蛋白质包围的一个中立类脂化合物核心组成。类脂化合物微滴的表面上的蛋白质对微滴结构和动力学关键。理解类脂化合物有大中央类脂化合物微滴的主要 adipocyte 的联系微滴的 proteome,从 C57BL/6 老鼠的白脂肪质的织物的类脂化合物微滴被孤立。,和由结合的液体层析双人脚踏车团 spectrometry。以前包括 73 的 193 蛋白质的一个总数 unreported 蛋白质被识别。而且,编码同位素的亲密关系标签(ICAT ) 被用来比较类脂化合物的差别在瘦的正常和高脂肪的导致食谱的肥胖的 C57BL/6 老鼠之间的联系微滴的 proteomes。23,蛋白质由 ICAT 分析确定了, 3 蛋白质是起来调整的, 4 蛋白质在从肥胖的老鼠的脂肪质的织物的类脂化合物微滴是下面调整的。重要地,类脂化合物微滴, perilipin A 和 vimentin 的二结构的蛋白质,极大地从肥胖的老鼠在脂肪质的织物的类脂化合物微滴被减少,为类脂化合物的含有的减少的蛋白质机械微滴稳定性。 | Yubo Ding Yibo Wu Rong Zeng Kan Liao | 2012 | Acta Biochimica et Biophysica Sinica2012，44，5： | 3 |
| 8 | Expression of orphan nuclear receptor NR4A2 in gastric cancer cells confers chemoresistance and predicts an unfavorable postoperative survival of gastric cancer patients with chemotherapy显示文摘 | Yifang Han Hui Cai Liye Ma Yibo Ding Xiaojie Tan Wenjun Chang Wei Guan Yan Liu Qiuxia Shen Yongwei Yu Hongwei Zhang Guangwen Cao | 2013 | Cancer2013，，19： | 2 |
| 9 | Characterization of SARS-CoV-2 worldwide transmission based on evolutionary dynamics and specific viral mutations in the spike protein显示文摘Background:The coronavirus disease 2019(COVID-19)caused by severe acute respiratory syndrome-related coronavirus-2(SARS-CoV-2)is pandemic.However,the origins and global transmission pattern of SARS-CoV-2 remain largely unknown.We aimed to characterize the origination and transmission of SARS-CoV-2 based on evolutionary dynamics.Methods:Using the full-length sequences of SARS-CoV-2 with intact geographic,demographic,and temporal information worldwide from the GISAID database during 26 December 2019 and 30 November 2020,we constructed the transmission tree to depict the evolutionary process by the R package'outbreaker'.The affinity of the mutated receptor-binding region of the spike protein to angiotensin-converting enzyme 2(ACE2)was predicted using mCSM-PPI2 software.Viral infectivity and antigenicity were tested in ACE2-transfected HEK293T cells by pseudovirus transfection and neutralizing antibody test.Results:From 26 December 2019 to 8 March 2020,early stage of the COVID-19 pandemic,SARS-CoV-2 strains identified worldwide were mainly composed of three clusters:the Europe-based cluster including two USA-based subclusters;the Asia-based cluster including isolates in China,Japan,the USA,Singapore,Australia,Malaysia,and Italy;and the USA-based cluster.The SARS-CoV-2 strains identified in the USA formed four independent clades while those identified in China formed one clade.After 8 March 2020,the clusters of SARS-CoV-2 strains tended to be independent and became'pure'in each of the major countries.Twenty-two of 60 mutations in the receptor-binding domain of the spike protein were predicted to increase the binding affinity of SARS-CoV-2 to ACE2.Of all predicted mutants,the number of E484K was the largest one with 86585 sequences,followed by S477N with 55442 sequences worldwide.In more than ten countries,the frequencies of the isolates with E484K and S477N increased significantly.V367F and N354D mutations increased the infectivity of SARS-CoV-2 pseudoviruses(P<0.001).SARS-CoV-2 with V367F was more sensitive to the S1-targeting neutralizing antibody than the wild-type counterpart(P<0.001).Conclusions:SARS-CoV-2 strains might have originated in several countries simultaneously under certain evolutionary pressure.Travel restrictions might cause location-specific SARS-CoV-2 clustering.The SARS-CoV-2 evolution appears to facilitate its transmission via altering the affinity to ACE2 or immune evasion. | Jiluo Liu Xi Chen Yan Liu Jiansheng Lin Jiaying Shen Hongwei Zhang Jianhua Yin Rui Pu Yibo Ding Guangwen Cao | 2021 | Infectious Diseases of Poverty2021，10，4： | 2 |
| 10 | Trastuzumab and oxaliplatin exhibit a synergistic antitumor effect in HER2-postive gastric cancer cells显示文摘 | Xiaodi Ding Xiujuan Qu Yibo Fan Xiaofang Che Jinglei Qu Ling Xu Jing Liu Yunpeng Liu | 2014 | Anti-Cancer Drugs2014，，3： | 2 |
| 11 | MicroRNA-25 promotes cell migration and invasion in esophageal squamous cell carcinoma显示文摘 | Xiaohui Xu Zhaoli Chen Xiaohong Zhao Jiwen Wang Dapeng Ding Zhen Wang Fengwei Tan Xiaogang Tan Fang Zhou Jian Sun Nan Sun Yibo Gao Kang Shao Ning Li Bin Qiu Jie He | 2012 | Biochemical and Biophysical Research Communications2012，，4： | 2 |
| 12 | BCR-ABL tyrosine kinase inhibitors in the treatment of Philadelphia chromosome positive chronic myeloid leukemia: A review显示文摘 | Xin An Amit K. Tiwari Yibo Sun Pei-Rong Ding Charles R. Ashby Zhe-Sheng Chen | 2010 | Leukemia Research2010，，10： | 1 |
| 13 | Multiporous Carbon Encapsulated Ni Nanoparticles Promoting Glycerol Valorisation towards Hydrogenation against Rearrangement显示文摘A dual-templating method was used to synthesize a series of hierarchical carbon supports containing different proportions of spherical macropores(ca.200 nm in diameter)and mesoporous channels(ca.4 nm in diameter).These and some other conventional carbon materials were subsequently impregnated with Ni and tested for the conversion of glycerol.The hierarchical catalysts exhibited a significantly higher conversion(96%)and selectivity(77%)to 1,2-propanediol,and the specificity selectivity coefficient(6.1)towards 1,2-propanediol against lactic acid was three times higher than that observed over a conventional Ni/Cmnicro catalyst(2.1).The enhanced performance of these materials,compared with the Ni nanoparticles supported on conventional carbon supports,was attributed to their high surface areas(>1110 m^2·g^-1)and large pore volumes(ca.0.4 cm^3·g^-1)permitting greater accessibility of substrate and/or intermediates to Ni active sites.Given that the concentration of accessible Ni sites in these materials is higher,a competitive benzilic-acid-rearrangement reaction to produce lactic acid was suppressed,leading to an enhanced hydrogenation selectivity to 1,2-propanediol.This study evidences the potential benefits,which can be established from utilizing hierarchical support materials in the valorization of biomass. | Bin Zhang Bin Chen Mark Douthwaite Lipeng Tang Yibo Zhang Meng Wang Ding Ma | 2020 | Chinese Journal of Chemistry2020，38，5： | 1 |
| 14 | A 3D QSAR Study of Betulinic Acid Derivatives as Anti-Tumor Agents Using Topomer Co MFA:Model Building Studies and Experimental Verification显示文摘 | Ding Weimin Sun Miao Luo Shaman Xu Tao Cao Yibo Yan Xiufeng Wang Yang | 2013 | Molecules2013，18，10： | 1 |
| 15 | Recent progress on the hydrogen storage properties of ZrCo-based alloys applied in International Thermonuclear Experimental Reactor(ITER)显示文摘Under the development of International Thermonuclear Experimental Reactor(ITER) system aimed at realizing the controllable fusion reaction to solve the energy crisis fundamentally, there is an urgent need to find an appropriate material for tritium handling. ZrCo alloy is considered to be a promising candidate for the storage and delivery of hydrogen isotopes due to the favorable characteristics such as low plateau pressure for absorption, high dissociation pressure at moderate temperature and better ability of trapping ~3He. However, the hydrogen induced disproportionation and the slower recovery/deliverty rate of ZrCo-based alloys have limited their further application in ITER system. This review summarizes the efforts towards enhancing the hydrogen storage properties of ZrCo-based alloys including element substitution, surface modification, disproportionation mechanism investigation and the isotope effect study. Element substitution and surface modification play positive role to improve the anti-disproportionation ability and kinetic property of the alloys. However, the ZrCo-based alloys require to be further modified by more attempts such as new composition, novelty modification method or catalyst addition in order to better satisfy the application demands for tritium handling. Moreover, new insight for further understanding the inner disproportionation mechanisms of this material is needed by combining the advance characterization and theoretical analysis, which is in favor of addressing the disproportionation problem of the ZrCo-based alloys essentially. | Feng Wang Rongfeng Li Cuiping Ding Wukui Tang Yibo Wang Shimeng Xu Ronghai Yu Ying Wu | 2017 | Progress in Natural Science:Materials International2017，27，1： | 1 |
| 16 | Corrosion and irradiation behavior of Fe-based amorphous coating in lead-bismuth eutectic liquids显示文摘Lead-bismuth eutectics (LBE) have considerable potential as a candidate material for accelerator-driven sub-critical systems(ADS).However,LBE corrosion and irradiation damage are two urgent challenges remaining to be solved for impellers of primary pumps.In this study,we have explored the possibility of using Fe-based amorphous coatings to overcome LBE corrosion and concurrently to sustain irradiation damage.Specifically,the Fe_(54)Cr_(18)Mo_(2)Zr_(8)B_(18)amorphous coating was prepared by high-velocity oxygen-fuel (HVOF) spraying on 316L steel and exposed to saturated oxygen static LBE for 500 h at 400℃.The coating with high thermal stability (T_(g)=615℃ and T_(x)=660℃) effectively prevented the substrate steel from being corroded by LBE owing to its unique long-range disordered atomic packing.The coating also exhibited strong irradiation resistance when being subjected to 45 dpa (displacement per atom) Au ion irradiation at room temperature,with no sign of crystallization even at the maximum implantation depth of 300 nm.Consequently,the hardness of the coatings before and after irradiation increased slightly.The current findings shed new insights into understanding corrosion mechanism and irradiation behavior of amorphous solids in LBE and expand the application range of amorphous materials. | LU ZhiChao PENG Xiang Yang TANG YuHai HAN ShuYin WU Yuan FU EnGang DING XiangBin HOU Shuo LU GuangYao CAO PeiPei ZHANG YiBo LIU XiongJun MA Dong WANG Hui JIANG SuiHe LU ZhaoPing | 2022 | Science China(Technological Sciences)2022，65，2： | 1 |
| 17 | Hepatitis B Virus Combo Mutations Improve the Prediction and Active Prophylaxis of Hepatocellular Carcinoma: A Clinic-Based Cohort Study显示文摘 | Jianhua Yin Junxue Wang Rui Pu Haiguang Xin Zixiong Li Xue Han Yibo Ding Yan Du Wenbin Liu Yang Deng Xiaowei Ji Ming Wu Min Yu Hongwei Zhang Hongyang Wang Timothy C. Thompson Wu Ni Guangwen Cao | 2015 | Cancer Prevention Research2015，，： | 1 |
| 18 | The Safe Distance Between Road Lighting Fixtures and Street Trees显示文摘The road lighting system and the road greening system, which are mutually interrelated and independent, are two important parts in the urban road environment. Unreasonable road lighting is easy to induce light pollution and has a great negative impact on the physiology and growth of garden plants in the urban green space. In this paper, 21 kinds of common tree species in the urban green space of Zhengzhou were selected as the research object, and the photosynthetic physiological parameters of landscape trees under the LED light source were observed using LI-6400 Photosynthesis System. This paper attempted to find the critical point for initiating photosynthesis of different types of tree species under a certain light source and then calculated the safe distance between lighting fixtures and landscape trees. The results showed that road lighting interfered with the photosynthetic physiological activities of the surveyed trees, affecting the normal dormancy of the plants at night; the sensitivity of different tree species to night lighting was different, and there were some differences in the light compensation points, so the corresponding safe distance was also different. It is hoped that this study can provide a valuable reference and scientific basis for urban road greening and lighting design. | PENG Yu ZHANG Haiyang GUO Kaikai DING Yibo WANG Xudong | 2019 | Journal of Landscape Research2019，11，2： | 0 |
| 19 | Monitoring vegetation drought in the nine major river basins of China based on a new developed Vegetation Drought Condition Index显示文摘The effect of global climate change on vegetation growth is variable.Timely and effective monitoring of vegetation drought is crucial for understanding its dynamics and mitigation,and even regional protection of ecological environments.In this study,we constructed a new drought index(i.e.,Vegetation Drought Condition Index(VDCI))based on precipitation,potential evapotranspiration,soil moisture and Normalized Difference Vegetation Index(NDVI)data,to monitor vegetation drought in the nine major river basins(including the Songhua River and Liaohe River Basin,Haihe River Basin,Yellow River Basin,Huaihe River Basin,Yangtze River Basin,Southeast River Basin,Pearl River Basin,Southwest River Basin and Continental River Basin)in China at 1-month–12-month(T1–T12)time scales.We used the Pearson's correlation coefficients to assess the relationships between the drought indices(the developed VDCI and traditional drought indices including the Standardized Precipitation Evapotranspiration Index(SPEI),Standardized Soil Moisture Index(SSMI)and Self-calibrating Palmer Drought Severity Index(scPDSI))and the NDVI at T1–T12 time scales,and to estimate and compare the lag times of vegetation response to drought among different drought indices.The results showed that precipitation and potential evapotranspiration have positive and major influences on vegetation in the nine major river basins at T1–T6 time scales.Soil moisture shows a lower degree of negative influence on vegetation in different river basins at multiple time scales.Potential evapotranspiration shows a higher degree of positive influence on vegetation,and it acts as the primary influencing factor with higher area proportion at multiple time scales in different river basins.The VDCI has a stronger relationship with the NDVI in the Songhua River and Liaohe River Basin,Haihe River Basin,Yellow River Basin,Huaihe River Basin and Yangtze River Basin at T1–T4 time scales.In general,the VDCI is more sensitive(with shorter lag time of vegetation response to drought)than the traditional drought indices(SPEI,scPDSI and SSMI)in monitoring vegetation drought,and thus it could be applied to monitor short-term vegetation drought.The VDCI developed in the study can reveal the law of unclear mechanisms between vegetation and climate,and can be applied in other fields of vegetation drought monitoring with complex mechanisms. | ZHAO Lili LI Lusheng LI Yanbin ZHONG Huayu ZHANG Fang ZHU Junzhen DING Yibo | 2023 | Journal of Arid Land2023，15，12： | 0 |
| 20 | Environmental hypoxia induces apoptosis in large yellow croaker Larimichthys crocea via both intrinsic and extrinsic pathways显示文摘Hypoxia has become an unfavorable factor affecting the sustainable development of the large yellow croaker Larimichthys crocea,an economically important mariculture fish in China.Apoptosis is a consequence of hypoxia on fish.However,the effects of hypoxia stress on apoptosis in L.crocea remain largely unknown.We investigated the effect of environmental hypoxia on apoptosis in L.crocea.Results show that hypoxia induced apoptosis in L.crocea both in vivo and in vitro.The mitochondrial membrane potential was significantly reduced in large yellow croaker fry(LYCF)cells.The expression levels of Bcell lymphoma/leukemia-2(Bcl-2)m RNA and protein were also significantly decreased in the liver and LYCF cells during 96 h and 48 h of hypoxia stress,respectively,whereas the expression level of Bcl-2 associated X(Bax)mRNA,Casp3 mRNA,and activity of caspase-3/7/9 were significantly increased,indicating that hypoxia induced caspase-dependent intrinsic apoptosis in L.crocea.The expression level of the apoptosis-inducing factor(AIF)protein was significantly increased in the liver and LYCF cells.The level of AIF protein was significantly decreased in the cytoplasm but increased in the nuclei of L.crocea,demonstrating that hypoxia induced the AIF-mediated caspase-independent intrinsic apoptosis.In addition,the activity of caspase-8 was significantly increased,indicating that hypoxia stress induced extrinsic apoptosis in L.crocea.Therefore,hypoxia induced apoptosis in L.crocea through both the intrinsic and extrinsic pathways.The present study accumulated basic biological information to help elucidate the mechanism of hypoxia response in marine fish. | Shengyu LUO Cheng LIU Xinming GAO Jingqian WANG Yibo ZHANG Jie DING Congcong HOU Junquan ZHU Bao LOU Weiliang SHEN Xiongfei WU Chundan ZHANG | 2023 | Journal of Oceanology and Limnology2023，41，6： | 0 |
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