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| 1 | Correction of a genetic disease by CRISPR-Cas9-mediated gene editing in mouse spermatogonial stem cells显示文摘Spermatogonial 干细胞(SSC ) 能在移植以后生产众多的男配偶子进接受者睾丸,介绍为基因治疗和修改基因的动物的连续生产的一条珍贵途径。然而, SSC 的成功的基因操作被限制了,部分由于复杂性和当前可得到的基因编辑技术的低效率。这里,我们证明有效基因修正能用 CRISPR-Cas9 系统被介绍进 SSC。我们使用了 CRISPR-Cas9 系统变异在 SCC 的 EGFP transgene 或内长的 Crygc 基因。变异的 SSC 在移植以后经历了精子发生进不肥沃的老鼠睾丸的生精的小管。圆 spermatids 被产生并且在进成熟卵母细胞的注射以后,支持了显示相应变异的显型的异质接合的后代的生产。而且,在 Crygc 的一个引起疾病的变化(在 SSC 先存在的 Crygc −/−) 能乐意地到加入的 CRISPR-Cas9-induced nonhomologous 结束(NHEJ ) 或指导相同的修理(HDR ) 被修理,导致没有是的离开目标修正的证据带改正的基因的 SSC 线由整个染色体的定序出现。用从这些线产生的圆 spermatids 的授精在 100% 的效率与改正的显型产生了后代。我们的结果表明有效基因由 CRISPR-Cas9 系统在老鼠 SSC 编辑,并且提供在 SSC 经由基因修正治好基因疾病的原则的证明。 | Yuxuan Wu Hai Zhou Xiaoying Fan Ying Zhang Man Zhang Yinghua Wang Zhenfei Xie Meizhu Bai Qi Yin Dan Liang Wei Tang Jiaoyang Liao Chikai Zhou Wujuan Liu Ping Zhu Hongshan Guo Hong Pan Chunlian Wu Huijuan Shi Ligang Wu Fuchou Tang Jinsong Li | 2015 | Cell Research2015，25，1： | 37 |
| 2 | Compound Danshen injection improves endotoxin-induced microcirculatory disturbance in rat mesentery显示文摘AIM: To investigate the effect of compound Danshen injection on lipopolysaccharide (LPS)-induced rat mesenteric microcirculatory dysfunctions and the underlying possible mechanism by an inverted intravital microscope and high-speed video camera system. METHODS: LPS was continuously infused through the jugular artery of male Wistar rats at the dose of 2 mg/kg per hour. Changes in mesenteric microcirculation,such as diameters of arterioles and venules,velocity of RBCs in venules,leukocyte rolling,adhesion and emigration,free radicals released from post-capillary venules,FITC- albumin leakage and mast cell degranulation,were observed through an inverted intravital microscope assisted with CCD camera and SIT camera. Meanwhile,the expression of adhesion molecules CD11b/CD18 and the production of free radical in neutrophils,and the expression of intercellular adhesion molecule 1 (ICAM-1) in human umbilical vein endothelial cells (HUVECs) were quantified by flow cytometry (FACS) in vitro. RESULTS: The continuous infusion with LPS resulted in a number of responses in microcirculation,including a significant increase in the positive region of venulestained with Monastral blue B,rolling and adhesion of leukocytes,production of oxygen radical in venular wall,albumin efflux and enhanced mast cell degranulation in vivo,all of which,except for the leukocyte rolling,were attenuated by the treatment with compound Danshen injection. Experiments performed in vitro further revealed that the expression of CD11b/CD18 and the production of oxygen free radical in neutrophils,and the expression of ICAM-1 in HUVECs were increased by exposure to LPS,and they were attenuated by compound Danshen injection. CONCLUSION: These results suggest that compound Danshen injection is an efficient drug with multi-targeting potential for improving the microcirculatory disturbance. | Jing-Yan Han Yoshinori Horie Soichiro Miura, Yasutada Akiba Jun Guo Dan Li Jing-Yu Fan Yu-Ying Liu Bai-He Hu Li-Hua An Xin Chang Man Xu De-An Guo Kai Sun Ji-Ying Yang Shu-Ping Fang Ming-Ji Xian Masahiro Kizaki Hiroshi Nagata Toshifumi Hibi | 2007 | World Journal of Gastroenterology2007，13，26： | 26 |
| 3 | Photodynamic therapy guidelines for the management of oral leucoplakia显示文摘With recent developments in photosensitizers and light delivery systems,topical 5-aminolevulinic acid-mediated photodynamic therapy (ALA-PDT) has become the fourth alternative therapeutic approach in the management of oral leucoplakia (OLK) due to its minimally invasive nature,efficacy,and low risk of systemic side effects and disfigurement.This report presents step-by-step guidelines for applying topical ALA-PDT in the management of OLK based on both the clinical experience of the authors and a systematic review of the current literature.Studies using protocols with standardized parameters and randomized clinical trials at multiple centres with adequate sample sizes and both interim and long-term follow-ups are needed before universally applicable guidelines can be produced in this field. | Qianming Chen Hongxia Dan Fan Tang Jiongke Wang Xiaoying Li Junxin Cheng Hang Zhao Xin Zeng | 2019 | International Journal of Oral Science2019，11，2： | 18 |
| 4 | The Chromosome-Based Rubber Tree Genome Provides New Insights into Spurge Genome Evolution and Rubber Biosynthesis显示文摘The rubber tree,Hevea brasiliensis,produces natural rubber that serves as an essential industrial raw material.Here,we present a high-quality reference genome for a rubber tree cultivar GT1 using single-molecule real-time sequencing(SMRT)and Hi-C technologies to anchor the~1.47-Gb genome assembly into 18 pseudochromosomes.The chromosome-based genome analysis enabled us to establish a model of spurge chromosome evolution,since the common paleopolyploid event occurred before the split of Hevea and Manihot.We show recent and rapid bursts of the three Hevea-specific LTR-retrotransposon families during the last 10 million years,leading to the massive expansion by~65.88%(~970 Mbp)of the whole rubber tree genome since the divergence from Manihot.We identify large-scale expansion of genes associated with whole rubber biosynthesis processes,such as basal metabolic processes,ethylene biosynthesis,and the activation of polysaccharide and glycoprotein lectin,which are important properties for latex production.A map of genomic variation between the cultivated and wild rubber trees was obtained,which contains~15.7 million high-quality single-nucleotide polymorphisms.We identified hundreds of candidate domestication genes with drastically lowered genomic diversity in the cultivated but not wild rubber trees despite a relatively short domestication history of rubber tree,some of which are involved in rubber biosynthesis.This genome assembly represents key resources for future rubber tree research and breeding,providing novel targets for improving plant biotic and abiotic tolerance and rubber production. | Jin Liu Cong Shi Cheng-Cheng Shi Wei Li Qun-Jie Zhang Yun Zhang Kui Li Hui-Fang Lu Chao Shi Si-Tao Zhu Zai-Yun Xiao Hong Nan Yao Yue Xun-Ge Zhu Yu Wu Xiao-Ning Hong Guang-Yi Fan Yan Tong Dan Zhang Chang-Li Mao Yun-Long Liu Shi-Jie Hao Wei-Qing Liu Mei-Qi Lv Hai-Bin Zhang Yuan Liu Ge-Ran Hu-tang Jin-Peng Wang Jia-Hao Wang Ying-Huai Sun Shu-Bang Ni Wen-Bin Chen Xing-Cai Zhang Yuan-Nian Jiao Evan E.Eichler Guo-Hua Li Xin Liu Li-Zhi Gao | 2020 | Molecular Plant2020，13，2： | 15 |
| 5 | A pulmonary source of infection in patients with sepsis-associated acute kidney injury leads to a worse outcome and poor recovery of kidney function显示文摘BACKGROUND:Hospital mortality rates are higher among patients with sepsis-associated acute kidney injury(SA-AKI)than among patients with sepsis.However,the pathogenesis underlying SA-AKI remains unclear.We hypothesized that the source of infection affects development of SA-AKI.We aim to explore the relationship between the anatomical source of infection and outcome in patients with SA-AKI.METHODS:Between January 2013 and January 2018,113 patients with SA-AKI admitted to our Emergency Center were identifi ed and divided into two groups:those with pulmonary infections and those with other sources of infection.For each patient,we collected data from admission until either discharge or death.We also recorded the clinical outcome after 90 days for the discharged patients.RESULTS:The most common source of infection was the lung(52/113 cases,46%),followed by gastrointestinal(GI)(25/113 cases,22.1%)and urinary(22/113,19.5%)sources.Our analysis showed that patients with SA-AKI had a significantly worse outcome(30/52 cases,P<0.001)and poorer kidney recovery(P=0.015)with pulmonary sources of infection than those infected by another source.Data also showed that patients not infected by a pulmonary source more likely experienced shock(28/61 cases,P=0.037).CONCLUSION:This study demonstrated that the source of infection infl uenced the outcome of SA-AKI patients in an independent manner.Lung injury may influence renal function in an asyet undetermined manner as the recovery of kidney function was poorer in SA-AKI patients with a pulmonary source of infection. | Yi-wen Fan Shao-wei Jiang Jia-meng Chen Hui-qi Wang Dan Liu Shu-ming Pan Cheng-jin Gao | 2020 | World Journal of Emergency Medicine2020，11，1： | 14 |
| 6 | Left atrial appendage morphology in patients with atrial fibrillation in China: implications for stroke risk assessment from a single center study显示文摘 | Kong Bin Liu Yu Hu He Wang Lei Fan Yang Mei Yang Liu Wanli Liao Jiafen Liu Dan Xing Dong Huang He | 2014 | Chinese Medical Journal2014，，24： | 12 |
| 7 | Identification of aberrant cell cycle regulation in Epstein-Barr virus-associated nasopharyngeal carcinoma by cDNA microarray and gene set enrichment analysis显示文摘 | Wenling Zhang Zhaoyang Zeng Yanhong Zhou Wei Xiong Songqing Fan Lan Xiao Donghai Huang Zheng Li Dan Li Minghua Wu Xiaoling Li Shourong Shen Rong Wang Li Cao Ke Tang Guiyuan Li | 2009 | Acta Biochimica et Biophysica Sinica2009，41，5： | 11 |
| 8 | Herbal medicine Yin Zhi Huang induces CYP3A4-mediated sulfoxidation and CYP2C19-dependent hydroxylation of omeprazole显示文摘瞄准:探索在殷·智·胡昂(YZH ) 和 omeprazole 之间的潜在的相互作用, CYP3A4 和 CYP2C19 的底层。方法:十八个健康志愿者包括 6 CYP2C19 *1 /*1, 6 CYP2C19 *1 /*2 和 6 CYP2C19 *2 /*2 在 2 阶段被注册,使随机化,转线路临床的试用。在每个阶段,志愿者收到了安慰剂或 10 mL YZH 口头的液体,为 14 d 3 倍每日。然后,所有病人口头上地拿了 20 mg omeprazole 囊。血样品在 omeprazole 管理以后被收集直到 12 h。omeprazole 和它的代谢物的血浆集中被 HPLC 与紫外察觉确定。结果:在 YZH 的治疗的 14 d 以后,血浆 omeprazole 显著地减少了,那些显著地 omeprazole 砜和 5-hydroxyomeprazole 增加了。在从到无穷的时间 0 的血浆集中时间曲线下面的区域的比率(到到 omeprazole 砜的 omeprazole 的 5-hydroxyomprazole 和那些的 omeprazole 的 AUC (0无穷)在 CYP2C19 *1 / *1 由64.80%+/-12.51%( P=0.001 )和63.31%+/-18.45%( P=0.004 )减少了,57.98%+/-14.80%( P=0.002 )和在 CYP2C19 *1 / *2 或 *3 的54.87%+/-18.42%( P=0.003 ),和37.74%+/-16.07%( P=0.004 )和在 CYP2C19 *2 / *2 的45.16%+/-15.54%( P=0.003 )分别地。到在 CYP2C19 *1 /*1 和 CYP2C19 *1 /*2 或 *3 的 5-hydroxyomprazole 的 omeprazole 的 AUC (0 无穷) 比率的减少比在 CYP2C19 *2 /*2 (P=0.047 和 P=0.009 ) 的那些大。结论:YZH 导致催化 CYP3A4 的 sulfoxidation 和在血浆 omeprazole 集中导致减少的 omeprazole 的 CYP2C19 依赖的 hydroxylation。 | Lan FAN Guo WANG Lian-sheng WANG Yao CHEN Wei ZHANG Yuan-fei HUANG Rui-xue HUANG Dong-li HU Dan WANG Hong-hao ZHOU | 2007 | Acta Pharmacologica Sinica2007，28，10： | 10 |
| 9 | A deep stratospheric intrusion associated with an intense cut-off low event over East Asia显示文摘European Centre for Medium-Range Weather Forecasts Re-Analysis Interim(ERA-Interim)reanalysis data and satellite data,and trajectory model were applied to analyze the dynamical,thermo-dynamical,and chemical structure in the upper troposphere and lower stratosphere(UTLS)of an intense cut-off low(COL)event occurring over East Asia during June 19-23,2010,and to characterize the process and transport pathway of deep stratospheric intrusion.The Atmospheric Infrared Sounder(AIRS)ozone data and the Global Positioning System Ozone(GPSO3)sonde data showed that the air mass originating from the polar formed a region with relatively high values of potential vorticity(PV)and ozone in the center of COL,and a secondary ozone peak appeared in the upper troposphere during mature stage of the COL.Forward trajectory simulation suggested that during the first stage of COL,deep stratospheric intrusion associated with strong northerly wind jet on the west side of the upper-level trough transported ozone-rich air from the polar lower stratosphere into the middle and lower troposphere in the mid-latitude,and increased the ozone concentration there.During the mature stage of the COL,stratospheric air was transported counterclockwise into the troposphere.Backward trajectory model was used to find the source regions of air mass within the COL during its mature stage.Model results show that air masses with high ozone concentration in the center of the COL have two source regions:one is the subpolar vortex which lies in northern part of Center Siberia,where ozone-rich air plays a major role in increasing the ozone concentrations,and the other is the strong shear region which is near by the cyclonic side of the extratropical jet axis(west of 90°E,near 50°N).The air masses with low ozone concentration around the COL also have two source regions:one is the anticyclonic side of the extratropical jet axis,where the air mass with the relatively low ozone concentration at the bottom of the COL is mainly controlled by horizontal movement,and the other is the warm area of the south side of COL,where the air mass on the east and west side of the COL is mainly dominated by upward motion. | LI Dan BIAN JianChun FAN QiuJun | 2015 | Science China Earth Sciences2015，58，1： | 10 |
| 10 | Chinese herbal medicine reduces mortality in patients with severe and critical coronavirus disease 2019: a retrospective cohort study显示文摘This study aimed to evaluate the efficacy of Chinese herbal medicine(CHM)in patients with severe/critical coronavirus disease 2019(COVID-19).In this retrospective study,data were collected from 662 patients with severe/critical COVID-19 who were admitted to a designated hospital to treat patients with severe COVID-19 in Wuhan before March 20,2020.All patients were divided into an exposed group(CHM users)and a control group(non-users).After propensity score matching in a 1:1 ratio,156 CHM users were matched by propensity score to 156 non-users.No significant differences in seven baseline clinical variables were found between the two groups of patients.All-cause mortality was reported in 13 CHM users who died and 36 non-users who died.After multivariate adjustment,the mortality risk of CHM users was reduced by 82.2%(odds ratio 0.178,95%CI 0.076–0.418;P<0.001)compared with the non-users.Secondly,age(odds ratio 1.053,95%CI 1.023–1.084;P<0.001)and the proportion of severe/critical patients(odds ratio 0.063,95%CI 0.028–0.143;P<0.001)were the risk factors of mortality.These results show that the use of CHM may reduce the mortality of patients with severe/critical COVID-19. | Guohua Chen Wen Su Jiayao Yang Dan Luo Ping Xia Wen Jia Xiuyang Li Chuan Wang Suping Lang Qingbin Meng Ying Zhang Yuhe Ke An Fan Shuo Yang Yujiao Zheng Xuepeng Fan Jie Qiao Fengmei Lian Li Wei Xiaolin Tong | 2020 | Frontiers of Medicine2020，14，6： | 10 |
| 11 | Zebrafish model for assessing induced organ toxicity by Strychnos nux-vomica显示文摘OBJECTIVE:To assess the acute organ toxicity of Strychnos nux-vomica with zebrafish model visually.METHODS:To assess acute toxicity,we initially determined the lethal concentration after Strychnos nux-vomica treatment for 24 h.Zebrafish was treated with five concentrations Strychnos nux-≦vo LC10 for 24 h,and the effects ofmica on morphology,function of heart,central nervous system,liver,kidney and organ-specific cell death were assessed.Next,we assessed the reversibility of toxic effect.RESULTS:Strychnos nux-vomica has an effect on the different organs of zebrafish,including heart,central nervous system,liver,and kidney,and cadiotoxicity induced by Strychnos nux-vomica was reversible to some extent.CONCLUSION:Zebrafish model is suitable for confirming the toxic target organs for Chinese traditional medicine. | Zhao Chongjun Tian Jinghuan Wang Jinfeng Feng Yaru Ni Yuanyuan Fan Jiaojiao Wang Chunmei Cao Dan Zou Qinwen Ma Zhiqiang Lin Ruichao | 2016 | Journal of Traditional Chinese Medicine2016，36，4： | 9 |
| 12 | Location Determinants of China's Outward Foreign Direct Investment显示文摘 | Shujie Yao Fan Zhang Pan Wang Dan Luo | 2017 | China & World Economy2017，25，6： | 8 |
| 13 | RNA-binding protein LIN28B inhibits apoptosis through regulation of the AKT2/FOXO3A/BIM axis in ovarian cancer cells显示文摘LIN28B is an evolutionarily conserved RNA-binding protein that regulates mRNA translation and miRNA let-7 maturation in embryonic stem cells and developing tissues.Increasing evidence demonstrates that LIN28B is activated in cancer and serves as a critical oncogene.However,the underlying molecular mechanisms of LIN28B function in tumorigenesis are still largely unknown.Here we report that LIN28B was expressed in over half of the patients with epithelial ovarian cancer who were examined(n=584).Functional experiments demonstrated that LIN28B inhibited ovarian cancer cell apoptosis.Furthermore,we showed that the proapoptotic factor BIM played an essential role in the antiapoptotic function of LIN28B.RNA-IP microarray analysis suggested that LIN28B binds to mRNAs that are associated with the DNA damage pathway,such as AKT2,in ovarian cancer cells.By binding to AKT2 mRNA and enhancing its protein expression,LIN28B regulated FOXO3A protein phosphorylation and decreased the transcriptional level of BIM,which antagonized the antiapoptosis activity of LIN28B.Taken together,these results mechanistically linked LIN28B and the AKT2/FOXO3A/BIM axis to the apoptosis pathway.The findings may have important implications in the diagnosis and therapeutics of ovarian cancer. | Xiaojuan Lin Jianfeng Shen Dan Peng Xinhong He Congjian Xu Xiaojun Chen Janos LTanyi Kathleen Montone Yi Fan Qihong Huang Lin Zhang Xiaomin Zhong | 2018 | Signal Transduction and Targeted Therapy2018，3，1： | 8 |
| 14 | Mechanism of Chaihu Shugan Powder(柴胡疏肝散) for Treating Depression Based on Network Pharmacology显示文摘Objective:To analyze the effective components of Chinese medicine(CM)contained in Chaihu Shugan Powder(柴胡疏肝散,CSP)in the treatment of depressive disorders and to predict its anti-depressant mechanism by network pharmacology.Methods:Absorption,distribution,metabolism,excretion,and toxicity calculation method was used to screen the active components of CSP.Traditional Chinese Medicine System Pharmacological Database Analysis Platform and text mining tool(GoPuMed database)were used to predict and screen the active ingredients of CSP and anti-depressive targets.Through Genetic Association Database,Therapeutic Target Database,and PharmGkb database targets for depression were obtained.Cytoscape3.2.1 software was used to establish a network map of the active ingredients-targets of CSP,and to analyze gene function and metabolic pathways through Database for Annotation,Visualization and Integrated Discovery and the Omicshare database.Results:The 121 active ingredients and 15 depression-related targets which were screened from the database can exert antidepressant effects by improving the neural plasticity,growth,transfer condition and gene expression of neuronal cell,and the raise of the expression of gap junction protein.The 15 targets passed 14 metabolic pathways,mainly involved in the regulation of neurotransmitters(5-hydroxytryptamine,dopamine and epinephrine),inflammatory mediator regulation of TRP channels,calcium signaling pathway,cyclic adenosine monophosphate signaling pathway and neuroactive ligand-receptor interaction and other signal channels to exert anti-depressant effects.Conclusion:This article reveals the possible mechanism of CSP in the treatment of depression through network pharmacology research,and lays a foundation for further target studies. | LIU Yuan-yue HU Dan FAN Qi-qi ZHANG Xiao-hao ZHU Yi-cheng NI Miao-yan WANG Yan-ming ZHANG Lan-kun SHENG Lei | 2020 | Chinese Journal of Integrative Medicine2020，26，12： | 6 |
| 15 | Mo-modified Pd/Al_2O_3 catalysts for benzene catalytic combustion显示文摘Mo-modified Pd/Al2O3catalysts were prepared by an impregnation method and tested for the catalytic combustion of benzene. The catalysts were characterized by N2 isothermal adsorption, X-ray diffraction(XRD), X-ray photoelectron spectroscopy(XPS), temperatureprogrammed desorption of NH3(NH3-TPD), H2temperature-programmed reduction(H2-TPR), and high-angle annular dark-field scanning transmission electron microscopy(HAADF-STEM). The results showed that the addition of Mo effectively improved the activity and stability of the Pd/Al2O3catalyst by increasing the dispersion of Pd active components, changing the partial oxidation state of palladium and increasing the oxygen species concentration on the surface of catalyst. In the case of the Pd–Mo/Al2O3catalyst,benzene conversion of 90% was obtained at temperatures as low as 190°C, which was 45°C lower than that for similar performance with the Pd/Al2O3catalyst. Moreover, the 1.0% Pd–5% Mo/Al2O3catalyst was more active than the 2.0% Pd/Al2O3catalyst. It was concluded that Pd and Mo have a synergistic effect in benzene catalytic combustion. | Zhanfeng He Zhanrong He Dan Wang Qifei Bo Ting Fan Yi Jiang | 2014 | Journal of Environmental Sciences2014，26，7： | 6 |
| 16 | Versatile Piriformospora indica and Its Potential Applications in Horticultural Crops显示文摘Modern horticultural crop production systems target the exploitation of sustainable techniques for crop improvement while maintaining balance with the environment. Several beneficial microbes have been investigated and have yielded remarkable results. The endophytic fungus, Piriformospora indica, is one microbe group with the potential to offer numerous benefits and opportunities in modern horticultural crop production. This plant growth-promoting mycorrhizal fungus is of particular interest due to its beneficial implications in plant growth and development. The fungus has been experimentally proven to significantly improve water and nutrient/mineral absorption, early flowering, seed production/germination and plant photosynthetic capability, growth rates;especially in nutrient-deprived soils, alter the production of secondary metabolites, and promote adaptation, tolerance and/or resistance to biotic and abiotic stressors. Its unique capability to be axenically cultured makes it feasible for research and application. These attributes in combinations with its broad host range, offer immense potential for this fungus for research in horticultural plant improvement. Thus, this review highlights the potential applications of P. indica in horticultural crops research and production. | Raphael Anue Mensah Dan Li Fan Liu Na Tian Xueli Sun Xiangyang Hao Zhongxiong Lai Chunzhen Cheng | 2020 | Horticultural Plant Journal2020，6，2： | 6 |
| 17 | KLF4 initiates sustained YAP activation to promote renal fibrosis in mice after ischemia-reperfusion kidney injury显示文摘Acute renal injury(AKI)causes a long-term risk for progressing into chronic kidney disease(CKD)and interstitial fibrosis.Yes-associated protein(YAP),a key transcriptional cofactor in Hippo signaling pathway,shuttles between the cytoplasm and nucleus,which is required for the renal tubular epithelial cells repair in the acute phase of AKI.In this study we investigated the role of YAP during ischemia-reperfusion(IR)-induced AKI to CKD.Mice were subjected to left kidney IR followed by removal of the right kidney on the day before tissue harvests.Mouse shRNA expression adenovirus(Ad-shYAP or Ad-shKLF4)and mouse KLF4 expression adenovirus(Ad-KLF4)were delivered to mice by intrarenal injection on D7 after IR.We showed that the expression and nucleus distribution of YAP were persistently increased until the end of experiment(D21 after IR).The sustained activation of YAP in post-acute phase of AKI was accompanied by renal dysfunction and interstitial fibrosis.Knockdown of YAP significantly attenuated IR-induced renal dysfunction and decreased the expression of fibrogenic factors TGF-βand CTGF in the kidney.We showed that the expression of the transcription factor KLF4,lined on the upstream of YAP,was also persistently increased.Knockdown on KLF4 attenuated YAP increase and nuclear translocation as well as renal functional deterioration and interstitial fibrosis in IR mice,whereas KLF4 overexpression caused opposite effects.KLF4 increased the expression of ITCH,and ITCH facilitated YAP nuclear translocation via degrading LATS1.Furthermore,we demonstrated in primary cultured renal tubular cells that KLF4 bound to the promoter region of YAP and positively regulates YAP expression.In biopsy sample from CKD patients,we also observed increased expression and nuclear distribution of YAP.In conclusion,the activation of YAP in the post-acute phase of AKI is implicated in renal functional deterioration and fibrosis although it exhibits beneficial effect in acute phase.Reprogramming factor KLF4 is responsible for the persistent activation of YAP.Blocking the activation of KLF4-YAP pathway might be a way to prevent the transition of AKI into CKD. | Dan Xu Pan-pan Chen Pei-qing Zheng Fan Yin Qian Cheng Zhuan-li Zhou Hong-yan Xie Jing-yao Li Jia-yun Ni Yan-zhe Wang Si-jia Chen Li Zhou Xiao-xia Wang Jun Liu Wei Zhang Li-min Lu | 2021 | Acta Pharmacologica Sinica2021，42，3： | 6 |
| 18 | A new distinct geminivirus causes soybean stay-green disease显示文摘Dear Editor,Plant viruses make up almost half of the plant disease-causing pathogens,affecting crop yields and the global economy(Savary et al.,2019).Soybean(Glycine max)is one of the most valuable legume crops in the world,supplying 25%of the global edible oil and two-thirds of the global concentrated protein for livestock feeding.Recently,the outbreak of soybean stay-green syndrome with delayed leaf senescence(stay-green),flat pods,and increased number of abnormal seeds has swept the soybean production in the Huang-Huai-Hai region of China,resulting in huge yield losses(Xu et al.,2019).This disease has become an epidemic and prominent problem in soybean production and is still expanding its geography,including North America,posing a serious threat to soybean production(Harbach et al.,2016;Zhang et al.,2016;Li et al.,2019).However,the cause of soybean stay-green syndrome remains obscure. | Ruixiang Cheng Ruoxin Mei Rong Yan Hongyu Chen Dan Miao Lina Cai Jiayi Fan Gairu Li Ran Xu Weiguo Lu Yu Gao Wenwu Ye Shuo Su Tianfu Han Junyi Gai Yuanchao Wang Xiaorong Tao Yi Xu | 2022 | Molecular Plant2022，15，6： | 6 |
| 19 | Caspase-11 promotes renal fibrosis by stimulating IL-1β maturation via activating caspase-1显示文摘Caspase-11 is a key upstream modulator for activation of inflammatory response under pathological conditions. In this study, we investigated the roles of caspase-11 in the maturation of interleukin-1β (IL-1β) and development of renal interstitial fibrosis in vivo and in vitro. Mice were subjected to unilateral ureteral obstruction (UUO). The mice were treated with either caspase-11 inhibitor wedelolactone (Wed, 30 mg/kg/day, ig) for 7 days or caspase-11 siRNA (10 nmol/20 g body weight per day, iv) for 14 days. The mice were euthanized on day 14, their renal tissue and blood sample were collected. We found that the obstructed kidney had significantly higher caspase-11 levels and obvious tubular injury and interstitial fibrosis. Treatment with Wed or caspase-11 siRNA significantly mitigated renal fibrosis in UUO mice, evidenced by the improved histological changes. Furthermore, caspase-11 inhibition significantly blunted caspase-1 activation, IL-ip maturation, transforming growth factor-p (TGF-P), fibronectin, and collagen I expressions in the obstructed kidney. Renal tubular epithelial NRK-52E cells were treated in vitro with angiotensin (Ang, 1 pmol/L), which stimulated caspase-11 activation and IL-ip maturation. Treatment with IL-1β (20ng/ml) significantly increased the expression of TGF-β, fibronectin, and collagen I in the cells. Ang ll-induced expression of TGF-β, fibronectin, and collagen I were suppressed by caspase-11 siRNA or Wed. Finally, we revealed using co-immunoprecipitation that caspase-11 was able to interact with caspase-1 in NRK-52E cells. These results suggest that caspase-11 is involved in UUO-induced renal fibrosis. Elevation of caspase-11 in the obstructed kidney promotes renal fibrosis by stimulating caspase-1 activation and IL-1β maturation. | Nai-jun Miao Hong-yan Xie Dan Xu Jian-yong Yin Yan-zhe Wang Bao Wang Fan Yin Zhuan-li Zhou Qian Cheng Pan-pan Chen Li Zhou Hong Xue Wei Zhang Xiao-xia Wang Jun Liu Li-min Lu | 2019 | Acta Pharmacologica Sinica2019，40，6： | 5 |
| 20 | 青年型开角型青光眼的中国家系的新型Myocilin基因突变显示文摘目的:探讨某中国家系青年型开角型青光眼(JOAG)的致病基因。
方法:在一个青光眼家系中,三代人中患JOAG或高眼压。我们筛查了6位家族成员Myocilin(MYOC)和 optineurin(OPTN)的突变和apolipoprotein E的多态性,2位患有JOAG,2位患有高眼压,2位无症状。正常对照组包括200位与本家系无关联的受试者。用编码野生型或变异性 MYOC 互补 DNA 的表达载体转染cos-7细胞系。细胞内或分泌的MYOC蛋白用Western法进行检测。 | Bao Jian Fan Dexter Y. L Leung Dan Yi Wang Stéphane Vincent Raymond Pancy O. S. Tam Dennis S. C. Lam Chi Pui Pang 王婷(译) | 2006 | 美国医学会眼科杂志(中文版)2006，18，4： | 5 |
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